Digital Commons Network^™

Mutant P53 Gain Of Function: Why Many See It, Why Some Do Not, Guillermina Lozano, Carol Prives, Kanaga Sabapathy

Faculty, Staff and Student Publications

Mutations in the TP53 tumor-suppressor gene in human cancer are unique in that 60% to 70% are of the missense variety, resulting in a full-length protein that is often highly expressed in patients' tumors. These missense mutant proteins often exhibit pro-oncogenic activities (referred to as gain of function) in mouse models and human cell lines and correlate with poor cancer prognosis in some cases.

A Novel Cardiomyopathy Phenotype Linked To A Chd7 Missense Variant, In Young Park, Chih-Wei Hsu, Karim Bouazoune, Christina E Espindola, Madeline Hannah Mclaughlin Armond, Cristian Coarfa, Sandra L Grimm, James F Martin, Donna M Martin, Cheryl Lyn Walker

Center for Medical Ethics and Health Policy Staff Publications

Loss of function in the chromatin remodeler CHD7 causes CHARGE syndrome, characterized by variable penetrance and diverse abnormalities. However, establishing genotype-phenotype correlations has been challenging, as most CHD7 inactivating mutations are null alleles. Through CHD7 missense variant analysis at potential phosphorylation sites, we identified T730 (T720 in mice) as a critical residue associated with pathogenesis. Using a CHD7 T730 missense variant (Chd7T720A) and a frameshift null allele (Chd7fs) in a mouse model, we found that Chd7fs/fs mice were non-viable, while Chd7fs/+ mice exhibited haploinsufficiency-related circling behavior. Notably, Chd7fs/T720A mice died before postnatal …

Correlations Within And Between Highly Multiplexed Proteomic Assays Of Human Plasma, Mary R Rooney, Jingsha Chen, Christie M Ballantyne, Ron C Hoogeveen, Eric Boerwinkle, Bing Yu, Keenan A Walker, Pascal Schlosser, Elizabeth Selvin, Nilanjan Chatterjee, David Couper, Morgan E Grams, Josef Coresh

Faculty, Staff and Students Publications

Introduction: The number of assays on proteomic platforms has grown rapidly. The leading platforms, SomaScan and Olink, have strengths and limitations. Comparisons of precision on the latest platforms-SomaScan 11k and Olink Explore HT-have not yet been established.

Methods: Among 102 participants in the Atherosclerosis Risk in Communities Study (mean age 74 years, 53% women, 47% Black), we used split plasma samples to measure platform precision. CV and Spearman correlations were calculated for each assay. Cross-platform agreement was assessed for overlapping proteins.

Results: SomaScan 11k demonstrated a median correlation of 0.85 for the 10 778 assays and a median CV of …

Oral Pcsk9 Inhibitors: Can Novel Therapies Overcome Barriers To Effective Ldl-Cholesterol Management?, Christie M Ballantyne, Alberico L Catapano, Khurram Nasir

Faculty, Staff and Students Publications

No abstract provided.

Phage-Induced Protection Against Lethal Bacterial Reinfection, Yikun Xing, Haroldo J Hernandez Santos, Ling Qiu, Samantha R Ritter, Jacob J Zulk, Rachel Lahowetz, Kathryn A Patras, Austen L Terwilliger, Anthony W Maresso

Faculty, Staff and Students Publications

Bacteriophages, or phages, are viruses that target and infect bacteria. Due to a worldwide rise in antimicrobial resistance (AMR), phages have been proposed as a promising alternative to antibiotics for the treatment of resistant bacterial infections. Up to this point in history, phage use in preclinical animal studies, clinical trials, and emergency-use compassionate care cases has centered around the original observation from 1915 showing phage as lytic agent, and thus a treatment that kills bacteria. Here, we describe an activity associated with phage therapy that extends beyond lytic activity that results in long-term protection against reinfection. This activity is potent, …

Adipocyte/Tumor Cell Crosstalk Via Igf-1/Txnip Axis Promotes Malignancy And Endocrine Resistance In Breast Cancer, Amanda Caruso, Felice Maria Accattatis, Cinzia Giordano, Luca Gelsomino, Piercarlo Del Console, Maria Francesca Fiorita, Balazs Gyorffy, Laura Bianchi, Alfonso Carleo, Rossana De Salvo, Bruno M Simões, Robert B Clarke, Lorenzo Memeo, Cristina Colarossi, Eleonora Aiello, Francesco Conforti, Suzanne A W Fuqua, Matilde Todaro, Giorgio Stassi, Catia Morelli, Diego Sisci, Daniela Bonofiglio, Stefania Catalano, Sebastiano Andò, Ines Barone

Faculty, Staff and Students Publications

Background: Despite significant improvements in the outcome of Estrogen Receptor (ER) α-positive breast cancer (BC) following the use of endocrine therapies, resistance remains a major challenge. Clinical studies proved that obesity, in addition to promote BC progression, is associated with a reduced efficacy to these treatments, but mechanisms remain unclear.

Methods: We used co-culture systems followed by validation through an ‘ex vivo’ model of human mammary obese (Ob) adipocytes and obese endocrine-resistant metastatic Patient-Derived Organoids (PDOs). Transcriptomics with MixOmics-MINT and MetaCore Functional Tools along with lentiviral and pharmacological approaches provide insights into mechanisms. Clinical relevance was investigated using public datasets, …

An Antibody Biomarker Associated With Onchocerca Volvulus Microfilariae Identified By Proteomic Analysis Of Parasite Tissues Isolated From Paraffin Embedded O. Volvulus Nodules By Laser Capture Microdissection, Sarah E Greene, Kerstin Fischer, Lucia S Di Maggio, Bruce A Rosa, Yuefang Huang, Irina Diekmann, Byoung-Kyu Cho, Jessica Lukowski, Young A Goo, Makedonka Mitreva, Nicholas Opoku, Gary J Weil, Peter U Fischer

2020-Current year OA Pubs

Onchocerciasis (river blindness), a neglected tropical disease caused by the filarial nematode Onchocerca volvulus, impacts millions of people in sub-Saharan Africa. The WHO coordinates global efforts to eliminate onchocerciasis and has prioritized development of improved diagnostic tests to aid these efforts. To find new microfilarial-associated diagnostic targets to help identify active infections, we used laser capture microdissection to isolate embryonic stages from histologic sections of O. volvulus worms in subcutaneous nodules excised from onchocerciasis patients. Proteomic analysis identified 2,512 O. volvulus proteins in those tissues, including 264 found only in the microfilariae (Mf). From this pool of diagnostic candidates, we …

Three Dimensional Multiscalar Neurovascular Nephron Connectivity Map Of The Human Kidney Across The Lifespan, Liam Mclaughlin, Bo Zhang, Siddharth Sharma, Amanda L Knoten, Madhurima Kaushal, Jeffrey M Purkerson, Heidie L Huyck, Gloria S Pryhuber, Joseph P Gaut, Sanjay Jain

2020-Current year OA Pubs

The human kidney maintains homeostasis through a complex network of up to a million nephrons, its fundamental tissue units. Using innovative tissue processing and light sheet fluorescence microscopy, we mapped the 3D neurovascular connectivity of nephrons to understand how their structural organization enables coordinated functions like filtration, absorption, and blood pressure regulation. Our analysis revealed developmental changes in glomerular orientation, density, volume, and innervation from birth through aging. We discovered an extensive nerve network connecting different nephron segments and organizing glomeruli into distinct communities. These communities are linked through "mother glomeruli" that serve as control centers, creating a repeating pattern …

Perceptions Of Spirituality And Of God: A Psychological Qualitative Study, Christopher E. Peck, Timothy B. Smith, Jamila Mastny

Faculty Publications

The majority of people living in the U.S. report that spirituality is an important part of their life that is influential to their well-being. However, individuals vary widely in how they conceptualize spirituality, which often stems from and overlaps with their conceptualizations of God or so-called “God image”. Examination of people’s experiences of spirituality and conceptualization of God can enable psychologists to improve their understanding of individuals’ core personal experiences. This study evaluates both spirituality and God image, as well as the relationship between these two constructs, using qualitative hermeneutic analysis of interviews with 63 adults (51% women, 49% men, …

Core Grade 7: Principles For Moving From Evidence To Recommendations And Decisions, Gordon Guyatt, Per Olav Vandvik, Alfonso Iorio, Arnav Agarwal, Liang Yao, Prashanti Eachempati, Linan Zeng, Derek K. Chu, Rohan D'Souza, Thomas Agoritsas, M. Hassan Murad, Stefan Schandelmaier, Jamie Rylance, Benjamin Djulbegovic, Victor M. Montori, Monica Hultcrantz, Romina Brignardello-Petersen

Peninsula Dental School

This seventh article in a seven part series presents the Core GRADE (Grading of Recommendations Assessment, Development and Evaluation) approach for moving from evidence to recommendations or policy decisions. Core GRADE users make strong recommendations for an intervention versus a comparator when the desirable consequences clearly outweigh the undesirable consequences, and a conditional (weak) recommendation when the balance is less clear. Primary considerations in deciding on recommendations considering an individual patient perspective include balance of benefits, harms, and burdens; the certainty of evidence; and values and preferences. Secondary considerations, most important from a population perspective, include costs, feasibility, acceptability, and …

Enhanced Heater For 3d Asic Chip Design, Na

Defensive Publications Series

This disclosure describes techniques to thermally characterize three-dimensional integrated circuits (IC). Per the techniques, logic is incorporated into the IC (chip-under-test) that can generate random data, e.g., pseudorandom binary sequence (PRBS), at controllable toggle rates. The random data toggles across the entire die. A power-control mechanism controls the toggle rate in a fine-grained manner. Thermal and voltage sensors integrated into the chip-under-test enable real-time monitoring of temperature and voltage and generate data that can be used to analyze the power consumption, thermal characteristics, and overall health of the chip.

A Century Of Servant Leadership: Jimmy Carter, Jeffrey H. Coats

Servant Leadership: Theory & Practice

This paper describes the servant leadership characteristics of Jimmy and Rosalyn Carter.

Enhancing Team Effectiveness: A Study On The Efficacy Of Servant Leadership Experiential Training As An Intervention, Jason R. Weber

Servant Leadership: Theory & Practice

Purpose: This paper aims to identify the impact of servant-leadership training on team effectiveness. It proposes using a perceptive behavior assessment tool (team effectiveness questionnaire (TEQ)), along with six servant leadership themes will increase a team’s effectiveness. This study aims to expand the literature on methods used when seeking to improve operational effectiveness of work teams.

Design/methodology/approach: This intervention study followed the two-group pre-test-post-test design. 18 teams were randomly assigned to either a control or experimental group. Each team completed the TEQ and identified one to two domains to focus their development on over an 8-week period. Experimental group teams …

Organizational Change, Servant Leadership, And Christian Perspective, David Macauley, Randall Trent Stevenson, Helen Mitchell, David S. Fowler

Servant Leadership: Theory & Practice

In today’s dynamic business environment, organizational change is inevitable, yet with a failure rate of more than 70%, successfully navigating change remains elusive for the majority of business leaders. Effective change management requires more than just strategy, it demands a leadership approach that fosters trust, engagement, and resilience among employees. Servant leadership, with its focus on humility, empathy, and support, offers a powerful framework for guiding leaders and the organizations they serve through transformative periods, and can increase the effectiveness of organizational change efforts. By putting the needs of others first, and aligning leadership actions with values such as patience, …

The Servant Leader's Influence, Kevin J. Hurt

Servant Leadership: Theory & Practice

No abstract provided.

Contributor Biographies, Editors Of Suurj

SUURJ: Seattle University Undergraduate Research Journal

No abstract provided.

Maghrebi Identity & Integration In France: Colonial Legacies And Contemporary Realities, Mariam Gohar

SUURJ: Seattle University Undergraduate Research Journal

Historical ties between France and the Maghreb continue to shape and inform the experiences of the Maghrebi population in contemporary French society. There is an extensive Maghrebi population in France as a result of France’s colonial history in the region and subsequent migration. The Maghrebi populations face structural and social barriers in French society, including employment and education disparities, spatial segregation, and social Otherization. Maghrebi communities are only one example of France’s structural discrimination against non-European populations. This work aims to unpack the historical, political, and economic factors that resulted in French colonization of the Maghreb and relate these to …

“This Is One Moment In Time, Sealed In Amber”: A Structural And Content Analysis Of Queer Characters And Themes In The Audio Drama Eos 10, Emma Renn

SUURJ: Seattle University Undergraduate Research Journal

Queer horror and science fiction (sf) audio dramas populate many podcasting platforms, from explicit calls for and queries about Queer representation to general forums discussing horror and sf audio dramas (or podcasts). In this study, I used the principles of a case study to explore the queer nature of EOS 10 (an sf audio drama). I listened to the podcast EOS 10 and coded transcriptions which focused on Queer themes, in reference to sexuality or in relationship to heteronormativity more generally. I focused primarily on the trope of found family, as well as how audio dramas can push back against …

Reframing Fat: Identifying Compulsory Thinness And The Role Of Discourse And Media In Its Perpetuation, Anabella Vucci

SUURJ: Seattle University Undergraduate Research Journal

Drawing on Judith Butler’s framework of compulsory heterosexuality, as well as Robert McRuer’s use of this framework in his own theory of compulsory able-bodiedness, this paper critiques the notion that thinness is merely a beauty standard. Rather, this paper argues that thinness is a marker of superiority that requires the subordination of fat individuals and is upheld by the historically informed, albeit fabricated, narrative that being thin is right. This paper asserts that thinness is compulsory—required from all bodies that are held to an unattainable standard of perfect thinness. Further, this paper examines the role that media play in the …

A Tale Of Two Cities: The Racial Disparities Of Air Pollution And Asthma In South Fresno, California, Alonso G. Lee, Caleb Stipkovits, Eve Wiseman

SUURJ: Seattle University Undergraduate Research Journal

This paper analyzes the relationship between PM2.5 pollution, asthma rates, and racial disparities in West, Central, and South-Central Fresno, California (WCSCF). Our findings reveal a moderate positive correlation between asthma prevalence and racial demographics, largely due to poor urban planning—for example, residential areas near high-traffic roads, industrial zones, limited green spaces, and political prioritization of economic development over health. The study identifies two policy gaps in California’s air quality legislation: First, SB 1000 has failed to hold Fresno accountable for addressing environmental justice concerns; Second, AB 617’s reallocation of Cap-and-Trade funds toward air quality monitoring does not tackle the root …

Suurj Volume 9 Entire Volume, Editors Of Suurj

SUURJ: Seattle University Undergraduate Research Journal

No abstract provided.

Volume 151, Issue 151, 2018, Megan Alfaro, Ursula K. Le Guin, Ahmed Moneim, Keenan Norris, Meg Tinsley, T.C. Boyle, Willy Lizarraga, Sophie Anne Berti, Sativa January, Susan Lowell, Brian Barrientez Ii, Steve H. Vu, Ellen Bass, James K. Zimmerman, Cb Follett, Joyce Schmid, James Brasfield, Meredith Davies Hadaway, Gustavo Hernandez, Marin Nelson, Silvia Bonilla, Katherine Morgan, Tommy Mouton, Sally Ashton, Anna Bernstein, Genevieve Deguzman, Suzanne Rico, Peyton Harvey, William Finnegan, Margaret Mcmullan, Don George, Clare Frank, Grace Schaffer, Robert D. Kirvel, Kelly Grogan, Kelley Jhung, Marisa Murrow, Terry Phimister, Sharon Simonson, Susan Shillinglaw, Talia Adry, Noelle Brada-Williams

Reed Magazine

No abstract provided.

Education In Advance Care Planning: A Novel Equity-Based, Interprofessional Service-Learning Approach, Dorothy Yam, Sriya Kudaravalli, Elizabeth A. Mulvaney, Leslie Scheunemann, Grace W. Orsatti

Journal of Innovation in Health Sciences Education

Introduction: Despite health sciences graduate schools emphasizing service-learning and interprofessionalism, they often struggle to integrate these values into the curricula effectively. Advance care planning is inherently interdisciplinary and presents an opportunity to address equity gaps through service-learning. This report describes a novel interdisciplinary service-learning initiative for graduate student trainees, focused on advance care planning in underserved communities.

Methods: After targeted training, teams of medical, law, and social work students, and professionals from their respective fields, took part in two Life Planning Fairs in historically underserved neighborhoods in Pittsburgh, Pennsylvania. Using a form developed by the Allegheny County Bar Association …

Letter To Cyrus Moore Dunham From E. W. Dunn

Cyrus Moore Dunham Papers

Letter to Cyrus Moore Dunham from E. W. Dunn wishing him to recover from his health issues and wishing him well.

Aga Khan University Suggested Calligraphy

Old name/logo items (letterheads, seals, posters, etc.)

No abstract provided.

Problem-Based Learning In Speech-Language Pathology Programs: A Scoping Review, Michelle P. Phoenix, Roohit Grewal, Kayla Brissette, Maya Albin, Minseo Kim

Teaching and Learning in Communication Sciences & Disorders

Purpose: Problem-based learning (PBL) promotes student-centered, active learning and has been applied in many health disciplines, including speech-language pathology (SLP). There is some SLP literature outlining how PBL has been applied and its components, however, how PBL is applied across SLP programs worldwide is yet to be explored. We therefore sought to answer the question, how do SLP programs apply PBL and what are the associated student outcomes?

Methods: Five databases were searched, as well as searching the grey literature for relevant articles. Covidence was used to de-duplicate, collate, and review articles. SLP program, study, PBL application, and student outcome …

Quality, Suitability, Understandability, Actionability, And Readability Of Patient Education Materials: Baseline Findings From Slp Graduate Students, Bonnie K. Slavych, Greg Williams, Lauren Siemers, Lauren E. Jones, Alana Mantie-Kozlowski

Teaching and Learning in Communication Sciences & Disorders

This study evaluated graduate SLP students’ ability to communicate complex health information, highlighting strengths and areas for improvement. A cross-sectional analysis of 28 first-semester graduate students enrolled in a voice disorders course examined the quality of patient education materials they created based on fictional scenarios. Quality and credibility were assessed using the DISCERN instrument, suitability with the Suitability Assessment of Materials, and understandability/actionability with the Patient Education Materials Assessment Tool for Printable materials. Readability was analyzed using five formulas: Flesch-Kincaid Grade Level, Gunning Fog Index, FORCAST, New Dale-Chall, and Simple Measure of Gobbledygook. DISCERN scores ranged from 17 to 31 …

Personalized Learning In A Pediatric Speech Sound Disorders Seminar, Paul W. Cascella

Teaching and Learning in Communication Sciences & Disorders

This paper highlights the utility of personalized learning (PL) embedded into an on-campus graduate seminar focused on pediatric speech sound disorders (PSSD). The example showcases six key PL features described from an autoethnographic lens. These include: (a) context-specific positionality viewpoints (i.e., instructor, student, discipline, and university); (b) PL-adapted course learning outcomes; and (c) PL-style instructional resources, (i.e., diverse and efficient elicitation study questions, intentionally introspective in-class activities, and a menu of student- and discipline-centric topics conveyed in varied instructional formats). Evidence of students’ PSSD accomplishments are shared via personalized proof portfolios, and links to entry level national certification benchmarks. Closing …

Foundations Of Interprofessional Practice: The Development Of A Keystone Course For An Interprofessional Collaborative Slp, Pt, And Ot Curricular Sequence, Kathleen M. Youse, Jill D. Black, Wendy L. Wachter-Schutz

Teaching and Learning in Communication Sciences & Disorders

The purpose of this paper is to describe one university’s experience in designing a foundational course that introduces the Interprofessional Education Collaborative (IPEC) Core Competencies (IPEC, 2016) and applies those competencies to interprofessional education and interprofessional practice experiences specific to the accreditation needs of three related graduate health science programs in Speech-Language Pathology, Physical Therapy, and Occupational Therapy. Four years of student evaluation data demonstrate that the foundational course effectively meets course objectives from the student perspective and provides valued opportunities to get to know student colleagues, begin to focus on effective collaboration, and gain insights into professions other than …

Case-Based Learning For Dysphagia Continuing Education: Participant Perceptions On Facilitators And Barriers, Kalea Jones, Sarah Szynkiewicz, Grayson Hill, Courtney Broadfoot, Kendrea L. Garand, Memorie M. Gosa

Teaching and Learning in Communication Sciences & Disorders

Case-based learning (CBL) is a valuable approach to healthcare education, fostering deeper learning, clinical reasoning, and decision-making skills, especially in complex medical conditions such as dysphagia. For speech-language pathologists who manage dysphagia, a CBL approach provides interactive hands-on learning opportunities. We examined the benefits and challenges of a CBL approach to dysphagia continuing education using participant feedback. Twenty participants who attended the Alabama Dysphagia Collective Conference in September 2023 in Tuscaloosa, AL completed a Likert-style survey evaluating attitudes and opinions toward a CBL conference design. Participants also provided open-ended feedback on the facilitators, barriers, clinical practice implementation, new skills learned, …