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أنماط التعلق كمنبئات بازدواجية التعبير الانفعالي لدى طلبة جامعة بغداد, Muawiyah Abu Ghazal, Riyam Mohammed Dawood Jun 2025

أنماط التعلق كمنبئات بازدواجية التعبير الانفعالي لدى طلبة جامعة بغداد, Muawiyah Abu Ghazal, Riyam Mohammed Dawood

Journal of the Association of Arab Universities for Research in Higher Education مجلة اتحاد الجامعات العربية للبحوث في التعليم العالي

هدفت الدراسة إلى الكشف عن القدرة التنبؤية لأنماط التعلق بازدواجية التعبير الانفعالي لدى طلبة جامعة بغداد، وفيما إذا كانت هنالك فروق ذات دلالة إحصائية في مستوى ازدواجية التعبير الانفعالي تعزى لمتغيري الجنس، والتخصص. تكونت عينة الدراسة من (400) طالب وطالبة من طلبة جامعة بغداد، خلال العام الدراسي 2023/2024، وقد اختيروا بالطريقة الميسرة. ولتحقيق أهداف الدراسة تم اعتماد مقياس ازدواجية التعبير الانفعالي لـ كنج وأمونس (King & Emmons, 1990)، ومقياس أنماط التعلق لدى الراشدين الذي طوره أبو غزال وجرادات (2009)، بعد التأكد من صدقها وثباتها. أظهرت نتائج الدراسة ان طلبة جامعة بغداد لديهم ازدواجية التعبير الانفعالي بدرجة متوسطة، في حين لم …

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Eng 1001g-600 College Composition I Critical Reading & Source-Based Writing, Lydia Craig Jun 2025

Eng 1001g-600 College Composition I Critical Reading & Source-Based Writing, Lydia Craig

Summer 2025

No abstract provided.

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Eng 1002g-600 College Composition Ii Argument & Critical Inquiry, Melissa Ames Jun 2025

Eng 1002g-600 College Composition Ii Argument & Critical Inquiry, Melissa Ames

Summer 2025

No abstract provided.

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Eng 4761-600 Advanced Nonfiction Writing, Amie Whittemore Jun 2025

Eng 4761-600 Advanced Nonfiction Writing, Amie Whittemore

Summer 2025

No abstract provided.

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Eng 4904-600 Studies In Film, Robert Martinez Jun 2025

Eng 4904-600 Studies In Film, Robert Martinez

Summer 2025

No abstract provided.

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Eng 5007-600 Composition Theory And Pedagogy, Rachael Ryerson Jun 2025

Eng 5007-600 Composition Theory And Pedagogy, Rachael Ryerson

Summer 2025

No abstract provided.

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Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao Jun 2025

Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao

Journal of Food Science and Biotechnology

[Objective ] This study explored the structural characteristics and in vitro antioxidant activity of yam polysaccharide-selenium nanoparticle (YP-SeNPs ) complexes.[Method ] Yam polysaccharide (YP) were obtained through hot water extraction and then purified by purification,diethylaminoethyl cellulose 52 (DEAE-52) column chromatography,and dialysis.With YP as a template,YP-SeNPs were prepared with different Se/YP mass ratios (1∶3,1∶1,and 4∶3) in an oxidation-reduction system comprising ascorbic acid (VC) and sodium selenite (Na 2SeO 3）.The complexes were characterized via multiple techniques,including visual observation of solution color,ultraviolet-visible absorption (UV-vis ) spectroscopy,particle size measurement,transmission electron microscopy (TEM ),scanning electron microscopy (SEM ),atomic force microscopy (AFM ),Fourier-transform infrared spectroscopy (FT-IR …

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Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang Jun 2025

Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang

Journal of Food Science and Biotechnology

Tea polyphenols are the general term for polyphenols in tea,mainly including catechins,flavonoids,and anthocyanins,which have antioxidant and antimicrobial properties.The author reviewed the structural characteristics and the antioxidant and antibacterial mechanisms of tea polyphenols.Additionally,the application of tea polyphenols in the processing and preservation of meat products is summarized,providing a theoretical basis for their use in meat products.Studies have shown that tea polyphenols can achieve anti-oxidation by scavenging free radicals,chelating metal ions,and inhibiting oxidase activity.Moreover,tea polyphenols can inhibit cell growth and proliferation by inhibiting the activities of enzymes involved in cell metabolism and damaging the cell membrane system.As natural antioxidants,tea polyphenols can significantly …

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Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao Jun 2025

Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao

Journal of Food Science and Biotechnology

[Objective ] This study explored the structural characteristics and in vitro antioxidant activity of yam polysaccharide-selenium nanoparticle (YP-SeNPs ) complexes.[Method ] Yam polysaccharide (YP) were obtained through hot water extraction and then purified by purification,diethylaminoethyl cellulose 52 (DEAE-52) column chromatography,and dialysis.With YP as a template,YP-SeNPs were prepared with different Se/YP mass ratios (1∶3,1∶1,and 4∶3) in an oxidation-reduction system comprising ascorbic acid (VC) and sodium selenite (Na 2SeO 3）.The complexes were characterized via multiple techniques,including visual observation of solution color,ultraviolet-visible absorption (UV-vis ) spectroscopy,particle size measurement,transmission electron microscopy (TEM ),scanning electron microscopy (SEM ),atomic force microscopy (AFM ),Fourier-transform infrared spectroscopy (FT-IR …

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Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju Jun 2025

Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare γ-aminobutyric acid (GABA )-enriched milk fermented with lactic acid bacteria and evaluate its anti-exercise fatigue effect.[Method ] High GABA-producing lactic acid bacteria were selected and used to ferment milk with a commercial starter culture.A mouse model of exercise fatigue was established by four weeks of exhaustive swimming training.After four weeks,the exhaustive swimming time,serum biochemical indicators,antioxidant indicators,and energy storage substance levels were measured and analyzed.[Result ] Lactobacillus plantarum (L.plantarum ) LP- 5 was selected as the strain for fermentation,and its GABA yield reached (1.340±0.003) g/L.The fermented milk showed the titratable acidity of (46.29±2.01) ºT,the …

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Virtual Dignitary Torts, Zahra Takhshid Jun 2025

Virtual Dignitary Torts, Zahra Takhshid

Sturm College of Law: Faculty Scholarship

The emergence of the metaverse and spatial computing, which has enabled immersive digital interactions, raise complex legal questions. This work examines the feasibility of addressing dignitary torts-such as battery and intentional infliction of emotional distress-committed via avatars. The particular challenge for tort law is the nonphysical nature of selfrepresentations in these virtual spaces. Drawing from the historical evolutions of several dignitary torts, such as the law of battery and emotional harm, this article argues that the key in allowing for the recognition of such harms is appreciating the expansion of the protection of physical body within these torts, to the …

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“They Don’T Want Us Anywhere”: The Impact Of Anti-Homelessness Laws On Unsheltered Residents Of Fort Worth, Texas, Brie Diamond Jun 2025

“They Don’T Want Us Anywhere”: The Impact Of Anti-Homelessness Laws On Unsheltered Residents Of Fort Worth, Texas, Brie Diamond

Qualitative Criminology (QC)

"Access to public space is not afforded equally amongst the population. Often, the people who must spend most of their time in these spaces, people experiencing homelessness, are barred by local or state law from being there. Fort Worth, Texas, implemented a camping ban in 2019 that prohibits camping on public or private property. This article explores the impact of this ban on unsheltered people experiencing homelessness using semi-structured interviews with 18 people residing in encampments across the city. The findings document the tangible and existential losses endured by this group and the impact on the perception of their place …

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Complexities Of Appellate Law: Experiences And Perspectives Of Utah Indigent Appellate Attorneys, Whitney Howey, Stephanie Kathan, Jeremiah W. Jaggers, Chris Cambron Jun 2025

Complexities Of Appellate Law: Experiences And Perspectives Of Utah Indigent Appellate Attorneys, Whitney Howey, Stephanie Kathan, Jeremiah W. Jaggers, Chris Cambron

Qualitative Criminology (QC)

"Indigent appellate attorneys experience high stress due to their unique roles and responsibilities. Very little scientific research examines the lives and experiences of attorneys in the workplace. Characterizing and describing the variety of complexities and challenges Utah appellate attorneys face within their work provides information towards filling the profession’s gaps, stressors, and occupational needs. A sample of five Utah appellate attorneys were interviewed to better understand their perspectives and experiences in the workplace. We used thematic analysis to identify themes related to Utah appellate attorney experiences and perspectives. We identified four major themes from the data, including Personal approach, Working …

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Indigenous Cultural Healing In The Pacific Northwest: The Columbia River Tribes And The Potential Return Of The Falls, Cj Senn Jun 2025

Indigenous Cultural Healing In The Pacific Northwest: The Columbia River Tribes And The Potential Return Of The Falls, Cj Senn

University Honors Theses

This thesis examines the historical, cultural, and ecological significance of Celilo Falls (Wyam), a once-sacred Indigenous site on the Columbia River, and the profound impacts of its submergence by The Dalles Dam in 1957. It looks into the history of the area as well as the potential for a return of the falls in the future. Drawing from a mixed-methods approach that includes a systematic literature review and qualitative interviews with Indigenous scholars, elders, and community members, the study explores how the loss of Celilo Falls disrupted Indigenous governance, economies, and spiritual practices. It further investigates how Celilo continues to …

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Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang Jun 2025

Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang

Journal of Food Science and Biotechnology

Tea polyphenols are the general term for polyphenols in tea,mainly including catechins,flavonoids,and anthocyanins,which have antioxidant and antimicrobial properties.The author reviewed the structural characteristics and the antioxidant and antibacterial mechanisms of tea polyphenols.Additionally,the application of tea polyphenols in the processing and preservation of meat products is summarized,providing a theoretical basis for their use in meat products.Studies have shown that tea polyphenols can achieve anti-oxidation by scavenging free radicals,chelating metal ions,and inhibiting oxidase activity.Moreover,tea polyphenols can inhibit cell growth and proliferation by inhibiting the activities of enzymes involved in cell metabolism and damaging the cell membrane system.As natural antioxidants,tea polyphenols can significantly …

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Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor, Keke Ma, Jiurui Fang, Huijie Zhang, Jinghua Chen Jun 2025

Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor, Keke Ma, Jiurui Fang, Huijie Zhang, Jinghua Chen

Journal of Food Science and Biotechnology

[Objective ] The aim of this study is to synthesize aminated glycogen (NG) for loading of the photosensitizer TTVBA and construct a nano drug delivery system (NGT NPs ) for photodynamic therapy (PDT ) of tumors.[Method ] Positively charged NG was synthesized through modification of glycogen with diethylenetriamine (DETA ).Then,TTVBA was encapsulated by NG via electrostatic interaction to prepare NGT NPs.The prepared NGT NPs were characterized by infrared spectroscopy,dynamic light scattering (DLS ),and transmission electron microscopy (TEM ).The hemolysis assay was performed to evaluate the biocompatibility of NGT NPs.The reactive oxygen species (ROS ) generation capability of cells treated with …

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Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan Jun 2025

Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare a highly specific antibody against channelrhodopsin- 1 (ChR 1) from Chlamydomonas reinhardtii (C.reinhardtii ) and investigate its regulatory mechanisms in cellular phototaxis and negative phototaxis.[Method ] The recombinant expression vector pET- 28a(+)-chr1 was constructed and transformed into Escherichia coli (E.coli ) BL21 (DE3) competent cells.The expression of the fusion protein 6×His∷ChR 1 was induced with IPTG,and the obtained protein was purified by nickel-affinity chromatography.The purified protein was used to immunize New Zealand white rabbits for the production of ChR 1 antiserum.ChR 1-specific IgG antibodies were enriched from the antiserum through protein A purification.The …

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Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen Jun 2025

Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen

Journal of Food Science and Biotechnology

[Objective ] This study aims to enhance the high osmotic pressure tolerance of Escherichia coli (E.coli ) for the efficient production of succinic acid.[Method ] Adaptive evolution,transcriptomic analysis,reverse metabolic engineering,and fermentation optimization were employed for improving E.coli tolerance to high osmotic pressure.[Result ] By analyzing the key factors influencing succinic acid production by fermentation,osmotic pressure was identified as the critical factor reducing succinic acid production efficiency.A mutant strain,E.coli FN-6,with tolerance to high osmotic pressure was obtained through adaptive evolution.The key gene cusC responsible for osmotic pressure tolerance was identified by transcriptomic analysis.After optimization of the expression level of cusC,the engineered …

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Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li Jun 2025

Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the detection specificity and sensitivity for Escherichia coli (E.coli ) O157:H7.[Method ] A high ly specific and sensitive method for the detection of E.coli O157:H7 was established based on magnetic separation and signal amplification strategies combined with surface-enhanced Raman spectroscopy (SERS ).Aptamers were used as recognition elements and combined with magnetic beads and polystyrene (PS) microspheres to construct a separation probe (MB@Apt ) and a signal amplification Raman tag (4-MBN@PS@Apt ),respectively.[Result ] In the presence of E.coli O157:H7,MB@Apt and 4-MBN@PS@Apt formed a “sandwich ” structured composite (MB@Apt/ E.coli O157:H7/4-MBN@PS@Apt ),which could be rapidly …

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Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo Jun 2025

Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo

Journal of Food Science and Biotechnology

[Objective ] A fluorescent immunochromatographic assay was established based on vitamin B 1 monoclonal antibody to measure the vitamin B 1 in infant formula milk powder.[Method ] The performance of vitamin B 1 monoclonal antibody was evaluated.On this basis,a highly sensitive fluorescent immunochromatographic test strip was developed.[Result ] The vitamin B 1 monoclonal antibody showed high sensitivity,affinity,and specificity.The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD ) of 10 μg/kg,a cut-off value of 200 μg/kg,and a calculated limit of detection (cLOD ) of 2.056 μg/kg.In addition,the recovery rate of this method was 97.3%~115.4%.The test results of the …

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Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods, Xiujin Chen, Biao An, Jian Tao, Xinhao Yin, Yao Wang, Huawei Niu, Zhaozhou Li, Min Zhang Jun 2025

Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods, Xiujin Chen, Biao An, Jian Tao, Xinhao Yin, Yao Wang, Huawei Niu, Zhaozhou Li, Min Zhang

Journal of Food Science and Biotechnology

[Objective ] A colloidal carbon-labelled immunochromatographic assay with competitive inhibition was developed for the detection of marbofloxacin (MBF ) residues in milk powder and chicken.[Method ] The anti-MBF polyclonal antibody prepared in the author 's laboratory was employed to produce the colloidal carbon-labelled immunoprobe.The effects of pH of reaction system,resuspension solution,treatment solution of sample pad,standard dilution buffer,mass concentration of colloidal carbon,mass concentration of primary antibody,mass concentration of coating antigen,and mass concentration of secondary antibody on the MBF colloidal carbon-labelled immunochromatographic assay were investigated.The sensitivity,specificity,stability,and accuracy of the method were determined.[Result ] Under optimal conditions,the cut-off value of the method was …

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Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju Jun 2025

Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare γ-aminobutyric acid (GABA )-enriched milk fermented with lactic acid bacteria and evaluate its anti-exercise fatigue effect.[Method ] High GABA-producing lactic acid bacteria were selected and used to ferment milk with a commercial starter culture.A mouse model of exercise fatigue was established by four weeks of exhaustive swimming training.After four weeks,the exhaustive swimming time,serum biochemical indicators,antioxidant indicators,and energy storage substance levels were measured and analyzed.[Result ] Lactobacillus plantarum (L.plantarum ) LP- 5 was selected as the strain for fermentation,and its GABA yield reached (1.340±0.003) g/L.The fermented milk showed the titratable acidity of (46.29±2.01) ºT,the …

Go to article

Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor, Keke Ma, Jiurui Fang, Huijie Zhang, Jinghua Chen Jun 2025

Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor, Keke Ma, Jiurui Fang, Huijie Zhang, Jinghua Chen

Journal of Food Science and Biotechnology

[Objective ] The aim of this study is to synthesize aminated glycogen (NG) for loading of the photosensitizer TTVBA and construct a nano drug delivery system (NGT NPs ) for photodynamic therapy (PDT ) of tumors.[Method ] Positively charged NG was synthesized through modification of glycogen with diethylenetriamine (DETA ).Then,TTVBA was encapsulated by NG via electrostatic interaction to prepare NGT NPs.The prepared NGT NPs were characterized by infrared spectroscopy,dynamic light scattering (DLS ),and transmission electron microscopy (TEM ).The hemolysis assay was performed to evaluate the biocompatibility of NGT NPs.The reactive oxygen species (ROS ) generation capability of cells treated with …

Go to article

Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan Jun 2025

Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare a highly specific antibody against channelrhodopsin- 1 (ChR 1) from Chlamydomonas reinhardtii (C.reinhardtii ) and investigate its regulatory mechanisms in cellular phototaxis and negative phototaxis.[Method ] The recombinant expression vector pET- 28a(+)-chr1 was constructed and transformed into Escherichia coli (E.coli ) BL21 (DE3) competent cells.The expression of the fusion protein 6×His∷ChR 1 was induced with IPTG,and the obtained protein was purified by nickel-affinity chromatography.The purified protein was used to immunize New Zealand white rabbits for the production of ChR 1 antiserum.ChR 1-specific IgG antibodies were enriched from the antiserum through protein A purification.The …

Go to article

Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen Jun 2025

Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen

Journal of Food Science and Biotechnology

[Objective ] This study aims to enhance the high osmotic pressure tolerance of Escherichia coli (E.coli ) for the efficient production of succinic acid.[Method ] Adaptive evolution,transcriptomic analysis,reverse metabolic engineering,and fermentation optimization were employed for improving E.coli tolerance to high osmotic pressure.[Result ] By analyzing the key factors influencing succinic acid production by fermentation,osmotic pressure was identified as the critical factor reducing succinic acid production efficiency.A mutant strain,E.coli FN-6,with tolerance to high osmotic pressure was obtained through adaptive evolution.The key gene cusC responsible for osmotic pressure tolerance was identified by transcriptomic analysis.After optimization of the expression level of cusC,the engineered …

Go to article

Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li Jun 2025

Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the detection specificity and sensitivity for Escherichia coli (E.coli ) O157:H7.[Method ] A high ly specific and sensitive method for the detection of E.coli O157:H7 was established based on magnetic separation and signal amplification strategies combined with surface-enhanced Raman spectroscopy (SERS ).Aptamers were used as recognition elements and combined with magnetic beads and polystyrene (PS) microspheres to construct a separation probe (MB@Apt ) and a signal amplification Raman tag (4-MBN@PS@Apt ),respectively.[Result ] In the presence of E.coli O157:H7,MB@Apt and 4-MBN@PS@Apt formed a “sandwich ” structured composite (MB@Apt/ E.coli O157:H7/4-MBN@PS@Apt ),which could be rapidly …

Go to article

Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo Jun 2025

Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo

Journal of Food Science and Biotechnology

[Objective ] A fluorescent immunochromatographic assay was established based on vitamin B 1 monoclonal antibody to measure the vitamin B 1 in infant formula milk powder.[Method ] The performance of vitamin B 1 monoclonal antibody was evaluated.On this basis,a highly sensitive fluorescent immunochromatographic test strip was developed.[Result ] The vitamin B 1 monoclonal antibody showed high sensitivity,affinity,and specificity.The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD ) of 10 μg/kg,a cut-off value of 200 μg/kg,and a calculated limit of detection (cLOD ) of 2.056 μg/kg.In addition,the recovery rate of this method was 97.3%~115.4%.The test results of the …

Go to article

Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods, Xiujin Chen, Biao An, Jian Tao, Xinhao Yin, Yao Wang, Huawei Niu, Zhaozhou Li, Min Zhang Jun 2025

Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods, Xiujin Chen, Biao An, Jian Tao, Xinhao Yin, Yao Wang, Huawei Niu, Zhaozhou Li, Min Zhang

Journal of Food Science and Biotechnology

[Objective ] A colloidal carbon-labelled immunochromatographic assay with competitive inhibition was developed for the detection of marbofloxacin (MBF ) residues in milk powder and chicken.[Method ] The anti-MBF polyclonal antibody prepared in the author 's laboratory was employed to produce the colloidal carbon-labelled immunoprobe.The effects of pH of reaction system,resuspension solution,treatment solution of sample pad,standard dilution buffer,mass concentration of colloidal carbon,mass concentration of primary antibody,mass concentration of coating antigen,and mass concentration of secondary antibody on the MBF colloidal carbon-labelled immunochromatographic assay were investigated.The sensitivity,specificity,stability,and accuracy of the method were determined.[Result ] Under optimal conditions,the cut-off value of the method was …

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Lawrence University One Hundred Seventy-Sixth Commencement, Sunday, June 15, 2025, Lawrence University Jun 2025

Lawrence University One Hundred Seventy-Sixth Commencement, Sunday, June 15, 2025, Lawrence University

Commencement Programs

No abstract provided.

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Advancing Novel Contrast Agents For Applications In Fluorescence-Guided Neurosurgery Using Fluorescence Cryotomography, Augustino Vittorio Scorzo Jun 2025

Advancing Novel Contrast Agents For Applications In Fluorescence-Guided Neurosurgery Using Fluorescence Cryotomography, Augustino Vittorio Scorzo

Dartmouth College Ph.D Dissertations

Fluorescence guided surgery (FGS) is an emerging surgical technique that aims to help surgeons identify tissue types to assist in surgical decision making - ideally providing “cut-by-color” guidance. A primary application for this approach is the identification of tumor tissue to achieve more accurate and complete tumor removal while simultaneously minimizing damage to surrounding normal tissue. The cornerstone of tumor visualization for FGS is the administration of fluorescent contrast agents, or in some cases metabolic precursors, designed to accumulate specifically in tumor tissue, and a wide range of agents that use different targeting mechanisms are currently under investigation. Although these …

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