Digital Commons Network^™

Characterization Of Growth, Motility And C-Di-Gmp In Selenomonas Sputigena Atcc 35185, Margaret E J Stiker

Theses and Dissertations

Selenomonas sputigena is an obligate anaerobic, Gram-negative pathobiont that transitions from a low-abundance commensal bacteria to a structural component in dysbiotic oral biofilms associated with early childhood caries and periodontitis. Analysis of S. sputigena ATCC 35185 cultures optimized in TYGVS media revealed robust growth, characteristic crescent morphology with flagellar tuft motility, and strong monospecies biofilm formation comparable to Streptococcus mutans via crystal violet assays.

ELISA quantification demonstrated significantly elevated c-di-GMP levels in sessile biofilm fractions relative to motile planktonic cells (p=0.0009), establishing the secondary messenger’s correlation with the motile-sessile lifestyle switch. Recombinant expression and purification yielded PilZ-domain effector proteins, Selsp_1666 …

Examining Bidirectional Associations Between Mothers’ Beliefs About Emotions And Child Social-Emotional Outcomes, Sydney Sumrall

Theses and Dissertations

Social-emotional (SE) skills encompass the ability to recognize and regulate emotions, manage behavior to act on goals, and maintain social relationships (Abrahams et al., 2019). Parents play a crucial role in child SE skill development. According to theories of emotion socialization (ES), the beliefs parents hold about emotions shape parents’ ES behaviors, including teaching children how to understand and express feelings (Eisenberg et al., 1998; Gottman, 1996). Because emotion socialization practices are influenced by cultural values (Raval & Walker, 2019), the parent beliefs and ES strategies that best support children’s SE skill development may vary across families’ racial backgrounds. Although …

Social And Community Determinants Of Alcohol Use Among Hispanic/Latino College Students: The Role Of Early Adversity And Drinking Motives, Isis A. Garcia-Rodriguez

Theses and Dissertations

Background. Alcohol use remains one of the most prevalent health-risk behaviors among college students in the United States and is associated with a range of academic, physical, and psychological consequences. Hispanic/Latino college students navigate complex social and cultural environments that may influence alcohol use behaviors, including exposure to early-life adversity, sociopolitical stressors, and culturally grounded sources of resilience. Guided by the Healthy People 2030 Social Determinants of Health (SDOH) framework, this study examined how social and community context factors relate to alcohol use among Hispanic/Latino college students and whether drinking motives function as a psychological mechanism linking social experiences to …

Environmental Monitoring In Shallow Lakes: Public Health Threats In Urban Waters, Kyleigh E. Johnson

Theses and Dissertations

Lakes are an integral part of urban and suburban communities, providing ecological, recreational, and public health benefits. Many of these lakes are located in densely populated areas and are subject to urban influences on water quality. Algal toxins and Escherichia coli are two common recreational concerns. Algal toxins come from harmful algal blooms that are influenced by nutrient availability and temperature, while bacteria are often introduced through run-off and animal waste. Sampling every 3-4 weeks from May-October 2025 allowed for the assessment of seasonal patterns and site-specific differences across nine lakes in the Richmond area. The highest bacterial concentrations appeared …

Mathematical Models With Clinical Applications For Improving Health Outcomes, Helen Harris

Theses and Dissertations

In clinical settings, patients are exposed to many risks and stressors that could result in adverse health outcomes. Here we present mathematical models that seek to address these risks. First, we present a Markov Chain model to investigate the effect of medication reconciliation (MR) completion on patient health outcomes in the intensive care unit. Using this model, we simulate the annual incidence of adverse drug events (ADEs) for three different ADE rates. Based on the simulated results, we conduct a cost-benefit analysis for various levels of compliance to determine the financial implications of increasing MR completion depending on the baseline …

Absolute Quantification And Identification Of Rna From Rna-Lipid Nanoparticles Using High Resolution Mass Spectrometry, Jason C. Funderburk

Theses and Dissertations

RNA therapeutics are a rising drug category with potential use for a range of conditions encompassing infectious diseases to therapies for cancer, diseases, and genetic disorders. RNA-lipid nanoparticles (RNA-LNPs) are the prominent delivery method for these therapeutics approved products include mRNA vaccines and polyneuropathy treatments. The emergency use authorizations and orphan drug status of current RNA-LNP drugs has allowed approval without finalization of the regulatory analytical procedures for quality monitoring. The objective of the study was to develop an LC-MS assay to simultaneously measure identity and concentration of two therapeutically relevant intact RNA constructs extracted from RNA-LNPs to enhance quality …

A Framework For Digital Energy Modulation And Translation In Projectional X-Ray Imaging, Richard R. Wargo

Theses and Dissertations

In projectional X-ray imaging, acquisition energy fundamentally influences subject contrast, image noise, and radiation dose. While dual-energy techniques exploit spectral differences to optimize contrast and enable material separation, acquiring multiple images of the same anatomy often requires additional exposures and increased system complexity.

This dissertation presents a framework for digital energy modulation (DEM), in which computational models are trained to translate projection images between energy domains without requiring additional X-ray acquisitions. DEM is conceived as a generalizable strategy for controlled contrast modulation in projection imaging, with potential applications in image optimization, radiation therapy alignment, and dual-energy applications.

The framework was …

The Impacts Of Mentoring On Mentor Teachers In A Teacher Residency Program, Eric J. Lindley

Theses and Dissertations

Teacher shortages across the United States have made educational researchers consider strategies for teacher retention. Teacher Residency Programs (TRPs) are a compelling focal area in which to study mentorship as it relates to teacher shortages and teacher retention because of their intentional preparation, development, and support for participating mentor teachers. For my dissertation study, I was interested in exploring mentors’ perceptions of their roles, relationships, and preparation as well as the impacts that mentoring had on them beyond what has already been reported in the literature. Using a qualitative interview design study approach, I conducted semi-structured interviews with 13 experienced …

Generating Synthetic Ct From Mri Data, Foysal Ahmed

Theses and Dissertations

Magnetic resonance imaging (MRI) provides excellent soft tissue contrast without ionizing radiation, making it a strong alternative to computed tomography (CT) in medical imaging workflows. However, CT remains essential for applications requiring electron density information, such as radiation therapy treatment planning. This study investigated the feasibility of generating synthetic CT (sCT) images from MRI using a deep learning-based U-Net architecture. A two-dimensional U-Net was trained on paired MRI-CT data from the SynthRAD 2025 dataset, consisting of 120 T1-weighted (T1W) and 60 T2-weighted (T2W) axial cases, including deformed CT (dCT) aligned to MRI. Model testing used an independent dataset of 30 …

Magnetic Resonance Only Planning Validation Using Synthetic Ct By Mvision, Eric D. Brass Sr

Theses and Dissertations

Problems: Adaptive radiotherapy requires clinically practical methods for responding to patient-anatomy changes that occur between the original planning images and the day of treatment delivery. Registering a previously acquired computed tomography scan to a newly acquired magnetic resonance image can introduce cross-modality uncertainty and may delay replanning. This thesis evaluates whether synthetic computed tomography generated from same-day magnetic resonance imaging can reduce registration-related uncertainty while supporting rapid dose recalculation for fraction-level adaptation.

Procedure: Magnetic resonance imaging DICOM data were exported from the ViewRay MRIdian treatment planning system and uploaded to MVision for synthetic computed tomography generation. The synthetic computed tomography …

Development Of A Practical Lu-177 Activity Calibration Protocol For Clinical Nuclear Medicine: Dosimetric Impact Analysis And Implementation Tools, Azam Almolaiki

Theses and Dissertations

Lutetium-177 (Lu-177) is widely used in radionuclide therapy, where accurate activity measurement prior to administration is essential for safe and effective treatment delivery. However, variability in dose calibrator response due to measurement conditions and source geometry remains a practical challenge in routine clinical settings. This study presents a practical clinical calibration strategy under real-world constraints for Lu-177 activity measurement using standard nuclear medicine instrumentation. A vial-based approach was implemented using a well-type ionization chamber (Capintec CRC-15R) to evaluate measurement repeatability, temporal stability, and geometry dependence. All measurements were performed under consistent conditions with appropriate decay correction applied using the physical …

A Mutant Perfect, Amelia R. Baxter

Theses and Dissertations

A Mutant Perfect examines how embodied knowledge can be made visible and transmissible through ceramic figures. The research asks what forms emerge when making is understood as a mode of thinking rather than a vehicle for pre-formed ideas, and how those forms might activate somatic recognition in a viewer. The primary method is coil-built ceramic sculpture. Color is applied in layered coats and removed by hand-sanding to expose varying strata beneath, producing a speckled, worn surface. In a second approach, pigment is built directly into the clay body through coil construction, making color structural rather than applied. Narrative prose and …

Characterization Of Microrna Expression In Neuron-Enriched Extracellular Vesicles During Early Life, Allison Molen

Theses and Dissertations

Extracellular vesicles (EVs) are membrane-bound sacs released by cells that carry molecular cargo reflective of their cell of origin. EVs enriched using L1 cell adhesion molecule (L1CAM) as a marker of putative neuronal origin can cross the blood–brain barrier and provide a minimally invasive window into brain-related processes. Exosomal microRNA (miRNA) regulate gene expression and have been implicated in psychiatric disorders, including major depressive disorder (MDD). However, measuring and analyzing miRNA from L1CAM-positive EVs presents substantial analytical challenges due to low abundance and sparse detection across samples.

Plasma samples from participants recruited through the Mid-Atlantic Twin Registry (MATR) were used …

A Qualitative Analysis Of Teacher-Report Modifications Of A Tier 2 Evidence-Based Program In Elementary Schools After Implementation Supports Are Withdrawn, Navneet Kaur

Theses and Dissertations

Growing literature emphasizes the importance of examining modifications (i.e., responses to unforeseen challenges and deliberate changes to a program) made by implementers to understand the long-term outcomes of evidence-based programs (EBPs), particularly after formal implementation supports, such as training and coaching, are withdrawn. Although prior research has documented that they occur during implementation, less is known about whether modifications occur once external supports are no longer available, and if so, what types of modifications are made, and the rationale for them. This gap is especially salient in school settings for teacher-delivered interventions. To address this gap, this study used semi-structured …

Examining The Impact Of Trauma-Informed Mindfulness Meditation On Posttraumatic Growth Following Interpersonal Trauma: A Randomized Controlled Trial, Margaret Poandl

Theses and Dissertations

Research on interventions designed to increase posttraumatic growth (PTG) among interpersonal trauma survivors is limited. The purpose of this study was to examine the impact of a six-week trauma-informed mindfulness meditation intervention on PTG. Additionally, I examined outcomes of posttraumatic stress (PTS) and loneliness, as well as analyzed the variables of dispositional mindfulness, hope, spirituality, and self-compassion to understand their potential role as mechanisms of change within the intervention on PTG. To answer these research questions, I conducted a randomized controlled trial study with 113 adults from a community sample who reported experiencing interpersonal trauma. Utilizing linear mixed modeling and …

Commissioning & Daily Qa Of Converted Linac For Electron Flash, Tomaj Javidtash

Theses and Dissertations

Ultra-high dose rate (FLASH) radiotherapy has demonstrated promising normal tissue sparing in preclinical studies; however, the lack of standardized dosimetry, commissioning, and quality assurance (QA) methodologies remains a major barrier to its reproducible and safe implementation. In particular, conventional clinical commissioning and QA protocols are not directly applicable at FLASH dose rates due to challenges associated with detector response, dose-per-pulse effects, and beam control limitations.

This thesis presents the commissioning of a converted Varian 21EX linear accelerator for electron FLASH operation within the Division of Medical Physics at the Virginia Commonwealth University (VCU) Department of Radiation Oncology. Comprehensive beam characterization …

Within The Ruin: A Modern Brewery Rebirth, Karim Asarzadeh

Theses and Dissertations

This thesis project focuses on the revitalization of the historic ruins of the James River Steam Brewery in Richmond, Virginia. The project transforms the forgotten industrial structure into a modern brewery with a second-floor restaurant addition and community gathering spaces while preserving the historic masonry ruins. It showcases a holistic design approach that considers all aspects of the building, including its connection to the urban context and landscape, as well as its architecture, interiors, and furniture. The project demonstrates how a holistic design approach can create stronger spatial connections and more cohesive environments. In particular, it offers a strategy for …

Exploring, Understanding, And Supporting Academic Help-Seeking In Context, Molly L. Taylor

Theses and Dissertations

Conceptualized as a self-regulated learning (SRL) strategy, academic help-seeking is a fascinating behavior because it is self-directed while also reliant on social interactions with help-givers (Karabenick, 2004). What is known about help-seeking in academic settings largely revolves around individual traits, strategies, motivations, beliefs, and identities, and how each of these constructs influence help-seeking outcomes (Bornschlegl et al., 2020; Fong et al., 2023; Karabenick & Gonida, 2018). What is underexplored in these areas, however, is how features of the learning context interact with student-level factors to shape academic help-seeking behaviors and beliefs. This dissertation attempts to address some of these gaps …

In-Between Echoes, Jiayi Liang

Theses and Dissertations

In this dissertation, I explore memory, diaspora, labor, and intergenerational trauma through constructed photography and experimental film. Informed by Homi K. Bhabha’s concept of the Third Space, I examine how identity is shaped through displacement, cultural negotiation, and inherited memory. Drawing from my relationship with my grandmother and my experience as a Chinese woman living in diaspora, I consider labor as both survival and care, tracing how domestic gestures and emotional structures continue across generations. Through collage, reconstruction, and animation, I rework fragments of family archives, colonial landscape imagery, and personal photographs to address grief, racialized perception, belonging, and the …

Profile: More Is Morris, Communications And Marketing

Profile (1996-)

In this Issue: New Name and New Director for the AgCountry Challenge ; Salute to Scholarships ; Gift to Not Only Enhance Cougar Sports Center But Entire Community ; Lucy Imholte's Lasting Legacy Honored Through Sculpture ; From Dining Hall to Third Floor Behmler: Rodriguez '87 is Eighth Chancellor ; Partnership Pilots New Energy Storage Technology on Campus ; Faculty and Staff Awards ; Van Wey Named Fulbright Finalist ; UMN Morris Receives Carnegie Community Engagement Classification ; Public Lecture, Telescope Viewing Part of SCOPES Conference ; UMN Morris Adds $165.3 Million to Minnesota's Economy Each Year ; PBS Documentary …

Nest Eggs And Lifelines: The Overlooked Strain Of Economic Volatility On 401(K) Participants, Samantha Prince

Faculty Scholarship

Many Americans rely on defined contribution plans like 401(k) plans for retirement savings. These plans do not guarantee a fixed retirement benefit; rather, the benefit is based on accumulated contributions and investment performance. When the stock market drops, so do retirement account balances. When inflation hikes living expenses, money does not go as far. President Donald Trump's policies, including those associated with tariffs, are causing economic and resource volatility leading to financial hardship. Americans worry as they watch living costs increase and their retirement savings diminish. It is well known that retirees rely on 401(k) plan balances to fund their …

Embracing Imperfection And Stress As The Foundation For Growth, Excellence, And Well-Being In Dentistry, John W. Claytor Jr., Dds, Magd-Llsr, Facd

Journal of the American College of Dentists

Studies reveal that stress in dentistry begins early in training and may progress throughout a dentist’s career. Fear of managing patient dissatisfaction, demanding schedules, time constraints, staff issues, and the stressors of clinical dentistry all contribute to professional strain. Difficulties associated with perfectionistic tendencies, burnout, and mental health concerns remain ever-present challenges within the profession. This article discusses evidence-based strategies, coping skills, organizational approaches, and professional resources that may help mitigate the effects of stress in dentistry. Particular emphasis is placed on reducing stigma surrounding mental health care and supporting initiatives such as the Lorna Breen Heroes Foundation’s “Safe Haven” …

Revisiting Nasal Polyp Scoring In Clinical Trials: Interpretive Variability And The Need For Standardization, Leigh J. Sowerby, Babak Pourmomenarabi, Antonino Maniaci, Olivia Ginty, Joseph Khristian Han

Department of Otolaryngology (ENT) Faculty Publications

[Introduction] Borish et al. provided an excellent review highlighting methodological variability among the six pivotal Phase III trials [1]. One critical yet overlooked issue is the inconsistent application of the endoscopic nasal polyp score (NPS), a primary outcome measure in all trials.

Oropharyngeal Syphilis Presenting As Tongue Based Ulcer With Lymphadenopathy, Serena F. Pu, Kevin J. Carlson, Jonathan R. Mark, Benjamin J. Rubinstein

Department of Otolaryngology (ENT) Faculty Publications

Within the aerodigestive tract, syphilis presents with varied signs and symptoms, some of which may mimic malignancy or other disease processes. We present the case of a 29-year-old man with otalgia and odynophagia who was found on physical examination and office laryngoscopy to have a 4-cm ulcerative mass of the tongue base and cervical lymphadenopathy. While planning for tissue diagnosis, the patient’s serologies revealed positive treponemal antibody testing and elevated rapid plasma reagin (RPR) titers, confirming syphilitic infection, likely representing secondary syphilis. While awaiting operative biopsy, the patient received intramuscular penicillin with complete resolution of the mass and symptoms, and …

Depemokimab: A Twice Yearly Anti-Il 5 Biologic For Chronic Rhinosinusitis With Nasal Polyps (Crswnp) - A Narrative Review, Marn Joon Park, Joseph K. Han, Kent K. Lam

Department of Otolaryngology (ENT) Faculty Publications

Depemokimab is a monoclonal antibody that targets interleukin-5 with enhanced binding affinity and an extended terminal elimination half‑life of 6-8 weeks. The unique pharmacodynamic features of depemokimab enable sustained suppression of type 2 inflammation with twice‑yearly subcutaneous dosing. This narrative review provides details of depemokimab's molecular profile, pharmacokinetics, and pharmacodynamics and results regarding the clinical efficacy and safety of depemokimab from recently published Phase 3 clinical trials for the treatment of chronic rhinosinusitis with nasal polyposis (CRSwNP). In 528 severe adult CRSwNP patients (ANCHOR‑1/‑2 trials), depemokimab achieved Δ total endoscopic nasal polyp score by -0.70 (p <  .001) and Δ nasal obstruction verbal response scale by -0.24 (p = .003) at week 52, compared to placebo. Twice‑yearly 100 mg depemokimab provides durable eosinophil suppression and with a safety profile comparable to placebo in Phase 3 trials for CRSwNP. Future work should refine biomarkers and assess comparative cost‑effectiveness and long‑term adherence, safety, and effectiveness.

Dupilumab In Chronic Rhinosinusitis Without Nasal Polyps: Randomized Phase 2 Trial (Orion), Stella E. Lee, Anju T. Peters, Philippe Gevaert, Joseph K. Han, Claus Bachert, Francisco Moreira Da Silva, Andrés Rosenblüt, Chih-Chi Hu, Jennifer Maloney, Paolo Caferra, Adrianna Michalak, Andrew P. Fontenot, Lacey B. Robinson, Neelam A. Phadke

Department of Otolaryngology (ENT) Faculty Publications

BACKGROUND: Chronic rhinosinusitis (CRS) is often driven by type 2 inflammation and is characterized by nasal obstruction/ discharge, facial pain/pressure, and/or reduced smell, either with or without nasal polyps (CRSwNP or CRSsNP).

OBJECTIVE: To test whether dupilumab improves radiographic features in CRSsNP. Methods: ORION (NCT04678856), a phase 2, randomized, multicenter, double blind, placebo-controlled study, assessed dupilumab efficacy and safety in adults with uncontrolled CRSsNP. Patients were randomized 1:1 to dupilumab or placebo for 24 to 52 weeks. Endpoints included changes from baseline at week 24 in Lund–Mackay computed tomography (LMK-CT) score (dupilumab only [primary] and vs placebo [secondary]), sinus Total …

Development Of Dashboards And Procedures For Using Connected Vehicle Data To Prioritize Investments In Electric Vehicle Related Infrastructure, Jairaj C. Desai, Nathaniel J. Sturdevant, George Mccue, Darcy M. Bullock

JTRP Technical Reports

The National Electric Vehicle Infrastructure (NEVI) Formula Program and the Charging and Fueling Infrastructure Discretionary Grant Program together provide $7.5 billion to support states with building out a nationwide EV charging network. Indiana’s Charging the Crossroads program, funded through the NEVI program, plans to invest almost $100 million to build an EV charging network along Indiana interstates and highways. There is broad consensus among states, that these fast charging station investments should be based upon quantitative geospatial data with the objective of maximizing the impact of the NEVI program investments.

However, traditional methods for monitoring electric vehicle (EV) usage have …

Embodied, Relational, And Rooted: A Social Work Portfolio, Deneen Pancari

Graduate Student Portfolios, Professional Papers, and Capstone Projects

This portfolio reflects my professional and personal growth over the past three years of the University of Montana!s (UM!s) Online Master of Social Work (MSW) Program and showcases my understanding of advanced social work competencies (University of Montana School of Social Work, n.d.). My background includes yoga facilitation and preschool education, where I developed a strong appreciation for early childhood development and mindfulness. I never imagined how this path would lead me into clinical work in eating disorder recovery, adolescent therapy, and somatic-based interventions, which focus on the connection between the mind and body. My MSW coursework and practicum experiences …

Ryn's Portfolio, Ryn Briggs

Graduate Student Portfolios, Professional Papers, and Capstone Projects

No abstract provided.

Practicing From The Inside Out: A Portfolio Of Trauma-Informed And Spirit-Led Social Work, Cailey R. Denboer

Graduate Student Portfolios, Professional Papers, and Capstone Projects

This portfolio represents the integration of academic learning, practicum experience, and personal growth throughout my Master of Social Work education at the University of Montana. Grounded in empowerment theory, trauma-informed care, and anti-oppressive practice, this work reflects my commitment to ethical, culturally humble, and relational social work across individual, community, and organizational contexts. Drawing from coursework and practicum experiences, I highlight the development of core competencies through applied work.

A central theme of this portfolio is the ongoing process of self-reflection and humility, particularly in recognizing areas of growth and navigating the tensions between personal values, professional responsibilities, and systemic …