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Articles 38611 - 38640 of 1791766
Full-Text Articles in Entire DC Network
Research Progress Of Anthocyanin-Loaded Aquatic Product Freshness Indicator Films, Zhao Zunle, Li Yun, Li Yu, Suo Jintie, Zhang Chun
Research Progress Of Anthocyanin-Loaded Aquatic Product Freshness Indicator Films, Zhao Zunle, Li Yun, Li Yu, Suo Jintie, Zhang Chun
Food and Machinery
Anthocyanin-loaded freshness indicator films can intuitively reflect the freshness of aquatic products through color changes. They are pH-sensitive and have characteristics such as natural safety and antioxidant properties. Although there have been a large number of studies on them, they still face huge challenges in aspects such as color development, stability, and sensitivity. This paper mainly summarizes the research on natural anthocyanins in pH-sensitive indicator films and their application in monitoring the freshness of aquatic products. The advantages of anthocyanins as indicators are analyzed, and measures to improve the performance of anthocyanin-loaded indicator films are further discussed. The focus is …
Research Progress Of Non-Thermal Food Processing Technologies, Wei Wenqiang, Bi Hanqing, Xiao Zhaoyin, Pang Guibing, Li Mingying, Zhang Hui
Research Progress Of Non-Thermal Food Processing Technologies, Wei Wenqiang, Bi Hanqing, Xiao Zhaoyin, Pang Guibing, Li Mingying, Zhang Hui
Food and Machinery
Non-thermal food processing technology, as an emerging technology in the food industry, has increasingly attracted attention and plays an important role in improving food nutrition, safety, and quality. This paper focuses on the working principles, mechanisms of action, and applications of non-thermal food processing technologies, including six mainstream methods, i.e., high-voltage electric field, magnetic field, ultra-high pressure, plasma, ultrasound, and radiation. It also discusses the current difficulties and challenges faced by non-thermal food processing technologies and analyzes their future development trends.
Research Progress On The Main Nutritional Active Components And Health Functions Of Hickory, Gao Junlong, Wang Chunxiao, Gao Fei, He Kan, Jin Chao, Wu Weijie, Deng Yangyong
Research Progress On The Main Nutritional Active Components And Health Functions Of Hickory, Gao Junlong, Wang Chunxiao, Gao Fei, He Kan, Jin Chao, Wu Weijie, Deng Yangyong
Food and Machinery
Hickory is a natural, pollution-free wild nut that grows in specific natural environments. It is highly favored by consumers for its uniquely crisp and fluffy texture, rich nutritional value, and multiple health benefits. This article provides a comprehensive analysis of the main nutritional and bioactive components of hickory, including proteins and amino acids, fats and fatty acids, vitamins, minerals, dietary fiber, polyphenols, squalene, and phytosterols.These components are compared with those of commonly consumed nut products to clarify the content levels of the primary bioactive nutrients in hickory. It also focuses on the bioactivities and recent research progress related to hickory …
Intelligent Online Detection Method For Food Packaging Defects Based On Improved Deep Learning, Shen Gao, Wang Zijian, Wang Xiaofeng, Li Ruiwen
Intelligent Online Detection Method For Food Packaging Defects Based On Improved Deep Learning, Shen Gao, Wang Zijian, Wang Xiaofeng, Li Ruiwen
Food and Machinery
[Objective] To propose an intelligent detection method that balances detection accuracy and speed for the problems of low manual detection efficiency, high missed and false detection rates, and insufficient recognition ability of traditional machine vision algorithms for complex texture packaging and small defects in packaging defect detection at current food production lines. [Methods] Using Swin Transformer as the core feature extraction module, this study utilizes its modeling ability for global image information and multi-scale feature fusion advantages to accurately capture defect features, such as small wrinkles and printing offsets on the packaging surface. Simultaneously, the YOLOv 12 fast detection framework …
The Relationships Between Ego Depletion And Social Behaviors Across Different Degrees Of Psychological Flexibility, Jacob Cage Norris
The Relationships Between Ego Depletion And Social Behaviors Across Different Degrees Of Psychological Flexibility, Jacob Cage Norris
Masters Theses
Social behavior includes behaviors that are influenced, directly or indirectly, by the realistic, anticipated, imagined, or implied presences of others. Particular social behaviors such as altruism or aggression may vary in how accessible they are, depending on the degree of self-control that has been recently exerted. Specifically, self-control fatigue, or ego depletion, seems to make aggression more likely and altruism less. However, individuals who are psychologically flexible, or who tend to be in contact with the present moment while engaging in behaviors that support the pursuit of values may not respond to ego depletion with the same changes to social …
Feeding Performance And Survival Of Louisiana Poeciliid Fishes Exposed To Residential Wastewater Effluent, Jonathan Chapman
Feeding Performance And Survival Of Louisiana Poeciliid Fishes Exposed To Residential Wastewater Effluent, Jonathan Chapman
Masters Theses
Mosquitoes are a primary transmitter of vector-borne disease, and their larvae can thrive within shallow pools of stagnant water. Rainwater drainage ditches are a common sight in many communities, and in Louisiana, they are dually used as outlets for onsite residential wastewater discharge in neighborhoods disassociated from municipal sewage treatment. However, when discharge systems malfunction, the water becomes uninhabitable to mosquito predators, allowing mosquitoes to thrive. This research examines the predation performance of mosquitofish (Gambusia affinis), sailfin mollies (Poecilia latipinna), and least killifish (Heterandria formosa), the primary biological control agents utilized to combat mosquitoes within ditch communities. Individuals were captured …
The Role Of Isotocin In Female Zebrafish (Danio Rerio) Reproductive Behavior And Its Implication For Intranasal Oxytocin Therapy In Humans, Katelynn M. Benge
The Role Of Isotocin In Female Zebrafish (Danio Rerio) Reproductive Behavior And Its Implication For Intranasal Oxytocin Therapy In Humans, Katelynn M. Benge
Masters Theses
In the past several decades, oxytocin has been colloquially termed the “cuddle hormone,” due to its role in pair-bonding and monogamy (Carter et al., 1992). In addition to being used experimentally for psychological disorder, it has also been used in couple’s counseling settings (Anagnostou et al., 2014; Domes et al., 2016). The outcomes of this research reveal mixed results, creating a need for further research in the topic to understand the exact mechanisms by which oxytocin works in the brain to induce this prosocial response. This study sought to address this deficit through the zebrafish animal model. The zebrafish serves …
Classroom To Campus: A Qualitative Case Study Of Exemplary First-Year Seminar Instructors In Student Transitions At A Louisiana University, Brianna J. Doucet
Classroom To Campus: A Qualitative Case Study Of Exemplary First-Year Seminar Instructors In Student Transitions At A Louisiana University, Brianna J. Doucet
Doctoral Dissertations
This qualitative case study examined how exemplary first-year seminar (FYS) instructors design and implement pedagogical strategies that support students’ transitions into higher education through the lens of Schlossberg’s (1981) Transition Theory. Conducted at a public university in Louisiana, the study explored how university-identified, award-winning instructors foster academic, social, and emotional adjustment among first-year students, a critical priority amid the impending enrollment cliff and the growing need to retain enrolled students. Using semi-structured interviews and thematic analysis, findings revealed exemplary instructors design intentional, relational, and reflective learning environments. They view transition as a shared institutional process, model adaptability and lifelong learning, …
Examining Alumni Engagement Policies Among R1 Universities: A Comparative Analysis Of Best Practices, Howayda Ackleh
Examining Alumni Engagement Policies Among R1 Universities: A Comparative Analysis Of Best Practices, Howayda Ackleh
Doctoral Dissertations
This study explores the need to update policies and enhance practices for alumni engagement in higher education institutions. Current alumni strategic plans require evaluation and refinement, while new approaches must be developed to strengthen alumni involvement. By analyzing the challenges institutions face in maintaining alumni connections, this review seeks to identify gaps and establish effective best practices. By examining the questions, how do alumni associations at select public R1 institutions apply Utilization-Focused Evaluation (UFE) principles to assess and improve their alumni engagement strategies, and how does this impact their alignment with institutional goals and objectives? This study aims to evaluate …
Faculty, Staff, And Counselor Awareness Of The Challenges Faced By Autistic Students: A Quantitative Descriptive Study Of Resources, Training, And Support For Autistic Student Success In Higher Education, Erika Rosario
Doctoral Dissertations
Higher education institutions should establish two essential measures to support students with autism spectrum disorder (ASD): they need to implement standardized training for all personnel working on campus and create opportunities for hands-on experience through peer programs and collaborative educational events. The pursuit of equity in education demands nothing less. This study investigates the extent to which university faculty, staff, and counselors are aware of the challenges faced by autistic students, how knowledgeable they are about resources available to aid them, and the degree of their training, support, and collaboration in addressing students’ needs. By answering these research questions, the …
State Of California Cannabis Reporting, University Of Mississippi – National Center For Cannabis Research And Education
State Of California Cannabis Reporting, University Of Mississippi – National Center For Cannabis Research And Education
California
No abstract provided.
Prevalence Of And Factors Associated With Nurse Burnout In The Us, Megha K. Shah, Nikhila Gandrakota, Jeannie P. Cimiotti, Neena Ghose, Miranda Moore, Mohammed K. Ali
Prevalence Of And Factors Associated With Nurse Burnout In The Us, Megha K. Shah, Nikhila Gandrakota, Jeannie P. Cimiotti, Neena Ghose, Miranda Moore, Mohammed K. Ali
Individual Articles
IMPORTANCE Clinician burnout is a major risk to the health of the US. Nurses make up most of the health care workforce, and estimating nursing burnout and associated factors is vital for addressing the causes of burnout.
OBJECTIVE To measure rates of nurse burnout and examine factors associated with leaving or considering leaving employment owing to burnout. DESIGN,
SETTING, AND PARTICIPANTS This secondary analysis used cross-sectional survey data collected from April 30 to October 12, 2018, in the National Sample Survey of Registered Nurses in the US. All nurses who responded were included (N = 50 273). Data were analyzed …
Advanced Practice Registered Nurse Burnout In Magnet Hospitals: Opportunities For Organizational Intervention, Lori Kennedy, Heather Brom, Karen B. Lasater, Nancy M. Albert, Barbara Holmes Gobel, Christi Delemos, Lisa M. Williams, Linda H. Williams, Linda H. Aiken
Advanced Practice Registered Nurse Burnout In Magnet Hospitals: Opportunities For Organizational Intervention, Lori Kennedy, Heather Brom, Karen B. Lasater, Nancy M. Albert, Barbara Holmes Gobel, Christi Delemos, Lisa M. Williams, Linda H. Williams, Linda H. Aiken
Individual Articles
OBJECTIVE: To determine modifiable organizational factors associated with advanced practice RN (APRN) burnout in Magnet®-designated hospitals to guide organizational interventions to improve APRN well-being and retention.
METHODS: Cross-sectional study of work environments of 50 US Magnet hospitals and the associated well-being of 21 855 of their clinicians (APRNs, physician assistants, physicians [MDs], RNs).
RESULTS: Overall, 37%of APRNs experienced high burnout compared with 34% of MDs (P < 0.05) and 49% of RNs (P < 0.001); additionally, 32%of APRNs intended to leave their jobs. APRN burnout was significantly lower in organizations that minimized chaotic working conditions, provided APRNs more control over their workloads, involved APRNs in shared governance, improved clinician and management relations, and improved interprofessional teamwork.
CONCLUSIONS:Well-being and retention of APRNs in Magnet hospitals require their greater engagement in organization processes and systems that improve work environments.
Personal And Work-Related Factors Associated With Nurse Resilience: An Updated Systematic Review Using Meta-Analysis And Narrative Synthesis, Fiona Yu, Deborah Raphael, Lisa Mackay, Melody Smith, Ritin Fernandez
Personal And Work-Related Factors Associated With Nurse Resilience: An Updated Systematic Review Using Meta-Analysis And Narrative Synthesis, Fiona Yu, Deborah Raphael, Lisa Mackay, Melody Smith, Ritin Fernandez
Individual Articles
Background: Nursing shortages and increased workforce turnover have significantly increased nurses’ workloads, thus increasing the risk of burnout and stress, particularly during the COVID-19 pandemic. A systematic review published in 2019 suggested that resilience could act as a buffer to adversity; however, the factors associated with resilience during and after this global pandemic have not been identified.
Objectives: To identify personal and work-related factors associated with nurse resilience, including the period during and after the COVID-19 pandemic.
Design: Systematic review.
Data Sources: The search was conducted between January and February 2024 in the following databases: CINAHL Plus, MEDLINE (Ovid), PsycINFO, …
The Food Ethics, Sustainability And Alternatives Course: A Mixed Assessment Of University Students’ Readiness For Change, Charles Feldman, Stephanie A. Navarro Silvera
The Food Ethics, Sustainability And Alternatives Course: A Mixed Assessment Of University Students’ Readiness For Change, Charles Feldman, Stephanie A. Navarro Silvera
Department of Nutrition and Food Studies Scholarship and Creative Works
Growing interest in food sustainability education aims to increase awareness of food distribution systems, environmental degradation, and the connectivity of sustainable and ethical food practices. However, recent scholarship has questioned whether such pedagogical efforts are meaningfully internalized by students or lead to sustained behavioral change. Prior studies document persistent gaps in students’ understanding of sustainability impacts and the limited effectiveness of existing instructional approaches in promoting transformative engagement. To address these concerns, the Food Ethics, Sustainability and Alternatives (FESA) course was implemented with 21 undergraduate and graduate students at Montclair State University (Montclair, NJ, USA). Course outcomes were evaluated using …
Environmental Degradations In Images: Analysis, Restoration, And Applications, Harsh Bhandari
Environmental Degradations In Images: Analysis, Restoration, And Applications, Harsh Bhandari
Doctoral Theses
This thesis investigates computational models and methodologies for restoring images degraded by challenging environmental conditions such as haze and underwater environments. It further explores the analysis and estimation of particulate matter (PM) concentration from both day and night scenes under varying weather conditions, using degraded visual data as a primary input. Each environment introduces distinct forms of visual degradation, making image restoration a critical challenge that directly impacts applications including visibility enhancement, weather analysis, particulate concentration estimation, and object detection. By addressing these challenges, this thesis aims to develop adaptable, data-driven solutions that enhance image clarity and improve information extraction …
Disease Burden Of Asthma Patients Utilizing Short-Acting Beta-2 Agonist-Only Inhalers As Rescue In The United States, Njira Lugogo, Rena C. Moon, Yen Chung, Joy David, Omar Motawakel, Ning A. Rosenthal, Neil Skolnik
Disease Burden Of Asthma Patients Utilizing Short-Acting Beta-2 Agonist-Only Inhalers As Rescue In The United States, Njira Lugogo, Rena C. Moon, Yen Chung, Joy David, Omar Motawakel, Ning A. Rosenthal, Neil Skolnik
Department of Family & Community Medicine Faculty Papers
Purpose: We aimed to describe the disease burden of asthma and examine relationships between short-acting beta-2 agonist (SABA) use and asthma exacerbations. Patients and Methods: This retrospective cohort study used geographically diverse Premier Healthcare Database (PHD) and linked insurance claims (10/01/2021-09/30/2022) for US patients aged ≥18 years with a SABA-only prescription and a history of asthma exacerbation within 12 months before index date (the earliest SABA prescription date). Three asthma control groups were defined based on SABA usage: well controlled-low (0–1 prescriptions), not well controlled-medium (2–3 prescriptions), very poorly controlled-high (≥4 prescriptions). Four asthma severity groups were defined using asthma …
Lived Experiences Of Caregivers Navigating The Individuals With Disabilities Education Act For Their Disabled Children, Natasha Weber Holmes
Lived Experiences Of Caregivers Navigating The Individuals With Disabilities Education Act For Their Disabled Children, Natasha Weber Holmes
Walden Dissertations and Doctoral Studies
The Individuals with Disabilities Education Act (IDEA) was designed to guarantee equitable access to special education services for children with disabilities. Yet, disparities persist in how caregivers navigate protections. They often face barriers related to limited legal knowledge, inconsistent school support, and systemic inequities, leaving many unable to fully advocate for their children. The purpose of this interpretative phenomenological analysis study was to explore lived experiences of caregivers as they engaged with the IDEA to secure services for their children with disabilities. Guided by the legal consciousness and empowerment theories, the study involved examining how caregivers interpret and act upon …
Deliver Us From Evil., Ethan Schimmoeller
Deliver Us From Evil., Ethan Schimmoeller
Ambulatory and Primary Care Articles
No abstract provided.
Hofgur Llc V. Rucker
All Decisions
In this holdover proceeding against an adult care facility resident, the court granted the tenant's guardian ad litem's post-judgment motion. The motion sought to stay execution of the warrant of eviction. The court stayed the warrant, conditioning its future execution on the landlord proving compliance with Department of Health regulations 18 NYCRR 487.4(m) and 18 NYCRR 487.5(f)(19). These regulations mandate that the landlord make persistent efforts to assist the tenant in securing appropriate alternative placement at a higher level of care, including filing five applications with other facilities.
Respiratory Health And Environmental Risks Among California Children: The Role Of Socioeconomic Status, Wen Chieh Chia
Respiratory Health And Environmental Risks Among California Children: The Role Of Socioeconomic Status, Wen Chieh Chia
Walden Dissertations and Doctoral Studies
Abstract Air pollution has been cited as a factor in the development of respiratory illness in children. Socioeconomic differences can intensify the effects of air pollution exposure on low-income children compared to higher-income children. This correlational study examined the relationship between air pollution exposure and respiratory health outcomes among California children, as well as the moderating role of socioeconomic status. The study was guided by the environmental health disparities framework and used secondary data from the California Environmental Health Tracking Program, analyzing data from 49 California counties (n = 49). Independent variables were PM2.5 concentrations and the number of ozone …
Costs And Cost-Effectiveness Of Improved Nurse Staffing Levels And Skill Mix In Acute Hospitals: A Systematic Review, Peter Griffiths, Christina Saville, Jane Ball, Chiara Dall'ora, Paul Meredith, Lesley Turner, Jeremy Jones
Costs And Cost-Effectiveness Of Improved Nurse Staffing Levels And Skill Mix In Acute Hospitals: A Systematic Review, Peter Griffiths, Christina Saville, Jane Ball, Chiara Dall'ora, Paul Meredith, Lesley Turner, Jeremy Jones
Individual Articles
Increasing registered nurse staffing and skill mix can be a net cost-saving solution to nurse shortages. Contrary to the strong policy push towards a dilution of nursing skill mix, investment in supply of RNs should become the priority.
Evidence-Based Practice Improves Patient Outcomes And Healthcare System Return On Investment: Findings From A Scoping Review, Linda Connor, Jennifer Dean, Molly Mcnett, Donna M. Tydings, Amanda Shrout, Penelope F. Gorsuch, Ashley Hole, Laura Moore, Roy Brown, Bernadette Mazurek Melnyk, Lynn Gallagher-Ford
Evidence-Based Practice Improves Patient Outcomes And Healthcare System Return On Investment: Findings From A Scoping Review, Linda Connor, Jennifer Dean, Molly Mcnett, Donna M. Tydings, Amanda Shrout, Penelope F. Gorsuch, Ashley Hole, Laura Moore, Roy Brown, Bernadette Mazurek Melnyk, Lynn Gallagher-Ford
Individual Articles
Background: Evidence-based practice and decision-making have been consistently linked to improved quality of care, patient safety, and many positive clinical outcomes in isolated reports throughout the literature. However, a comprehensive summary and review of the extent and type of evidence-based practices (EBPs) and their associated outcomes across clinical settings are lacking.
Aims: The purpose of this scoping review was to provide a thorough summary of published literature on the implementation of EBPs on patient outcomes in healthcare settings.
Methods: A comprehensive librarian-assisted search was done with three databases, and two reviewers independently performed title/abstract and full-text reviews within a systematic …
Ai-Assisted Tailored Intervention For Nurse Burnout: A Three-Group Randomized Controlled Trial, Gumhee Baek, Chiyoung Cha
Ai-Assisted Tailored Intervention For Nurse Burnout: A Three-Group Randomized Controlled Trial, Gumhee Baek, Chiyoung Cha
Individual Articles
Background: High-stress environments, heavy workloads, and the emotional demands of patient care, which are common challenges faced by nurses, are factors that can lead to burnout. Shift work can make traditional burnout interventions costly and difficult to implement. Artificial intelligence (AI) could offer solutions that are less constrained by time, resources, and labor.
Aim: To investigate the effectiveness of an AI-assisted intervention in reducing nurse burnout.
Methods: A single-blind, three-group, randomized controlled trial of 120 nurses (40 per group) was conducted from June 2023 to July 2023. The AI-assisted tailored intervention included two 2-week programs, delivering one of four programs …
Mediating Role Of Lifestyle Factors In The Resilience–Job Satisfaction Relationship Among Nurses: A Descriptive Correlational Study, Lena I. Serafin, Katarzyna Wesołowska-Gorniak, Angeli Malewska, Karolina Kruszewska, Justyna Kujawa, Bozena Czarkowska-Pączek
Mediating Role Of Lifestyle Factors In The Resilience–Job Satisfaction Relationship Among Nurses: A Descriptive Correlational Study, Lena I. Serafin, Katarzyna Wesołowska-Gorniak, Angeli Malewska, Karolina Kruszewska, Justyna Kujawa, Bozena Czarkowska-Pączek
Individual Articles
Given the global nursing shortage and the increasing prevalence of workplace stressors, resilience has emerged as a crucial factor influencing job satisfaction, preventing burnout, and ensuring high-quality patient care. While previous research has established a connection between resilience and various occupational outcomes, the mediating effect of health-promoting behaviors—such as physical activity, diet, and sleep—remains largely unexplored, underscoring the need for further investigation in this area. Therefore, the objective of this study is to examine the effect of chosen lifestyle aspects on the connection between resilience and job satisfaction among nurses.
Matched Unrelated Vs Haploidentical Donor Hematopoietic Cell Transplantation Using Posttransplant Cyclophosphamide, Dipenkumar Modi, Ramzi Abboud, Et Al.
Matched Unrelated Vs Haploidentical Donor Hematopoietic Cell Transplantation Using Posttransplant Cyclophosphamide, Dipenkumar Modi, Ramzi Abboud, Et Al.
2020-Current year OA Pubs
Posttransplant cyclophosphamide (PTCy)-based graft-versus-host disease (GVHD) prophylaxis is now standard for matched unrelated donor (MUD) hematopoietic cell transplantation (HCT). Previous studies comparing MUD and haploidentical donor HCT using PTCy were limited in size and follow-up. We therefore performed a registry-based analysis examining the impact of donor type on HCT with PTCy. Adult patients (n = 5873) receiving MUD (n = 1973) or haploidentical (n = 3900) HCT with PTCy for acute leukemia (74.2%) or myelodysplastic syndrome (MDS; 25.8%) reported to the Center for International Blood and Marrow Transplant Research between 2017 and 2021 were included. Primary end points were 3-year …
Induction Of Moderate Dna Damage Enhances Megakaryopoiesis And Platelet Production, Virginia Camacho, Luis Francisco Zirnberger Batista, Et Al.
Induction Of Moderate Dna Damage Enhances Megakaryopoiesis And Platelet Production, Virginia Camacho, Luis Francisco Zirnberger Batista, Et Al.
2020-Current year OA Pubs
Megakaryocytes (MKs) are large, hematopoietic cells with a polyploid, multilobulated nucleus. Although DNA replication in MKs (endomitosis) is well studied, limited investigations have examined the impact of DNA instability on megakaryopoiesis. Poly-adenosine diphosphate (ADP) ribose polymerase (PARP) inhibitors are chemotherapeutics that result in accumulation of DNA damage and are commonly associated with thrombocytopenia, presumably mediated through platelet progenitors, MKs. To explore PARP inhibitor-induced thrombocytopenia, we treated mice with the PARP inhibitor niraparib. Although high-dose niraparib treatment led to thrombocytopenia, consistent with clinical observations, lower-dose treatment led to a significant increase in bone marrow MKs, MK progenitors (MkPs), and circulating platelets. …
Differential Virulence And Immune Recognition Of Klebsiella Pneumoniae O-Antigen Subtypes O2Α And O2Β, Paeton L Wantuch, Lloyd S Robinson, Cory J Knoot, Christian M Harding, David A Rosen
Differential Virulence And Immune Recognition Of Klebsiella Pneumoniae O-Antigen Subtypes O2Α And O2Β, Paeton L Wantuch, Lloyd S Robinson, Cory J Knoot, Christian M Harding, David A Rosen
2020-Current year OA Pubs
No abstract provided.
New Comorbidity Index Associated With Survival After Chimeric Antigen Receptor T-Cell Therapy For Large B-Cell Lymphoma, Uri Greenbaum, Amanda Cashen, Et Al.
New Comorbidity Index Associated With Survival After Chimeric Antigen Receptor T-Cell Therapy For Large B-Cell Lymphoma, Uri Greenbaum, Amanda Cashen, Et Al.
2020-Current year OA Pubs
No abstract provided.
A Chemogenetic Approach For Temporal And Cell-Specific Activation Of Endogenous Gpcrs In Vivo, Gwendolyn Shingles, Qianqian Pang, Jian Weng, Ryan Singer, Waleed Babar, Jiaqi Shen, Luis Vazquez-Rivera, Yao Chen, Peng Li, Wenjing Wang
A Chemogenetic Approach For Temporal And Cell-Specific Activation Of Endogenous Gpcrs In Vivo, Gwendolyn Shingles, Qianqian Pang, Jian Weng, Ryan Singer, Waleed Babar, Jiaqi Shen, Luis Vazquez-Rivera, Yao Chen, Peng Li, Wenjing Wang
2020-Current year OA Pubs
Cell-specific regulation of endogenous G protein-coupled receptors (GPCRs) is crucial for understanding their roles in physiological processes. We present chemogenetic tools using shield-1-dependent irreversible protein switches to regulate peptide agonist activity. To demonstrate this platform, we engineered chemogenetically regulated pituitary adenylate cyclase activating polypeptide (cPACAP), which exhibited >15-fold chemical-dependent regulation of endogenous receptor activity. In vivo application of cPACAP allowed neuronal activation via the endogenous receptor for PACAP, engaging neural circuits that control respiratory and feeding behaviors. By integrating cPACAP with transgenic mice, we selectively activated endogenous PACAP receptor signaling in hypocretin-expressing neurons of the lateral hypothalamic area (LHA), revealing …