Digital Commons Network^™

Teacher Perceptions Of Hazing Among Middle & High School Students: A Phenomenological Study, Delisa A. Joseph

Doctoral Dissertations and Projects

The purpose of this transcendental phenomenological study was to explore teacher perceptions of hazing at the middle and high school levels. Additionally, this study explored coaches’, school counselors’, and school leadership team members’ experiences of hazing among middle and high school students. Relevant literature related to hazing within education, the comparison, and contrast of hazing and bullying, policies, group dynamics, imitation, and student perceptions were reviewed. The theory that guided this study was Albert Bandura’s 1986 Social Cognitive Theory, as it emphasizes social influence and external and internal social reinforcement. The research questions used were (1) What are teachers’ perceptions …

A Phenomenological Study: The Self-Efficacy Of Twice-Exceptional Students, Lisa Ann Glover

Doctoral Dissertations and Projects

The purpose of this transcendental, phenomenological study was to describe the perceived self-efficacy of twice-exceptional students in a singular school district located in the southeastern United States. The theories which guided this study are Swain and French’s affirmative model of disability, Deci and Ryan’s self-determination theory, and Bandura’s self-efficacy theory because students who view their disabilities in a positive sense may have greater self-determination and thus increased self-efficacy. The guiding research question for the study examined the shared perceptions of self-efficacy among twice-exceptional students. Data to examine this question were collected from 10 high school students at East Lake School …

A Phenomenological Study Examining The Experiences Of Single Parents Who Are Rearing Elementary-Aged Children Enrolled In A Public School In A Rural Community, Jenine Lavonne Cotman

Doctoral Dissertations and Projects

Abstract The purpose of this qualitative transcendental phenomenological study was to examine the experiences of single parents who are rearing elementary-aged children enrolled in a public school in a rural community. Vygotsky’s sociocultural theory of cognitive development was used to guide this study. Vygotsky’s theory focuses on cognitive development being fundamentally framed by social interaction. Vygotsky also believed that cognitive development is strengthened when a child is encouraged to learn within their zone of proximal development, which refers to a person’s current cognitive space that can be further developed with social interaction and help from a teacher, peer, or parent. …

Applying Biblical Teaching To Resolve Power Struggles Between The Pastor And Deacons In A Southern Baptist Church, Jason Oneal Sweeton

Doctoral Dissertations and Projects

As the Body of Christ, each person within the church must know their role and responsibility, especially the leadership. The Bible teaches that there are pastors, elders, and bishops referring to one office who have been given the authority to shepherd, or lead as it were, the church. The Bible teaches that deacons are an integral part of the church. They are to be servants in the church and minister to the needs of the congregation. The purpose of this project lies in the misconceptions surrounding the roles and biblical responsibilities of both deacons and pastors. Many churches simply follow …

Emotional Disconnection Between Parents And Adolescents: Communication Obstacles Among Immigrant Chinese American Christian Families, Agnes Yinshan Man

Doctoral Dissertations and Projects

The purpose of this transcendental phenomenological study was to understand and describe the lived experiences of communication that lead to emotional connection issues between Chinese immigrant parents and their American-born adolescent children, particularly in families attending Chinese American Christian churches in Massachusetts. The theory guiding this study was social constructivism, which posits that family members may interpret and experience the same interactions differently from one another. The research questions of the study include: (1) How do first-generation Chinese American Christian parents and second-generation Chinese American Christian adolescent children experience their interaction with each other? (2) How do American-born Chinese Christian …

The Effects Of Physical Fitness Policies On Law Enforcement Officers' Physical Fitness Levels, Tina M. Hall

Doctoral Dissertations and Projects

Most law enforcement agencies have minimum physical standards for new hires; however, few agencies have physical fitness standards for members once they are hired. Many law enforcement officers experience a decrease in physical fitness levels as their years of law enforcement service increase. The decrease in physical fitness levels causes a reduction in the ability to perform job duties and increases health consequences. This study explored the relationship of physical fitness levels of sworn law enforcement members from agencies with and without enforced physical fitness standards. Anonymous surveys were completed by 1240 sworn law enforcement officers from eight state law …

My Sister's Keeper: Using Vision Boards To Examine Cross-Racial Mentorship And Supervision Experiences Of Black Women Counselor Educators Who Attended Cacrep Ces Programs At Predominately White Institutions, Donnette A. Deigh

Doctoral Dissertations and Projects

This transcendental phenomenological study explored the lived experiences of Black women recent graduates from Council for Accreditation of Counseling & Related Educational Programs (CACREP) accredited counselor education and supervision (CES) doctoral programs regarding having a non-Black supervision mentor while attending their predominately White institution. The participants were eight CES doctoral students from PWIs across the United States, including the southeastern, western, and Midwestern regions. Data were obtained using semi structured interviews and vision boards identifying participants’ cross-racial mentorship and supervision experiences during their doctoral programs using critical race theory and Black feminist thought as the theoretical framework. Thematic analysis of …

Toward Transformative Christian Education That Facilitates Spiritual Formation At Miracle Temple Church Of God In Christ, Devale A. Hodge

Doctoral Dissertations and Projects

The discipleship or Christian education ministry at Miracle Temple Church of God in Christ (COGIC) is not fostering intentional spiritual formation within its congregants. Spiritual formation intentionality is missing within the congregation. The lack of spiritual formation intentionally naturally leads to a discipleship deficit. And this deficit is seen and felt within this project’s context. Yet the call to return to deep and transformative disciple-making, which produces Christlikeness is vital and commonplace. This thesis project attempts to examine and address the struggle of discipleship and spiritual formation intentionality at Miracle Temple COGIC. To address the problem the researcher implements a …

Exploring Parental Perceptions Concerning Sexual Communication Between Sub-Saharan African Immigrant Parents And Their Children, Kwame Owura Frimpong

Doctoral Dissertations and Projects

Abstract Sub-Saharan African families experience communication barriers due to factors such as sexual silence, cultural taboos, and shame, preventing them from having sexual health communication with their children. The purpose of this study was to explore the perceptions of Sub-Saharan African immigrant parents concerning sexual shame and parents’ attitudes, knowledge, and comfort level in having a conversation with their pre-adolescent children concerning sexuality and sexual practices. While numerous researchers have demonstrated efficiency in audience-specific interventions, the literature reflects little attention to African immigrants, specifically Sub-Saharan immigrants. The research questions addressed perceptions of influences of a decision to discuss issues relating …

Teacher-Student Relationships Influencing Classroom Management Of Challenging Behaviors: A Case Study, Kristen Slifko

Doctoral Dissertations and Projects

The purpose of this case study was to understand how teacher-student relationships influence classroom management of students with challenging behaviors for teachers of ninth- grade Multi-tiered System of Supports (MTSS) students at Blue High School. The theory guiding this study was Glasser’s choice theory. All human behavior is driven by the desire to satisfy the five basic needs: survival, freedom, fun, power, and love. Behaviors happen because of personal choices made in an attempt to fulfill those needs. This idea served as a framework for all research and data collection. The method used for this case study was qualitative with …

Hypothermia For Patients Requiring Evacuation Of Subdural Hematoma: A Multicenter Randomized Clinical Trial, Georgene W Hergenroeder, Michael N Diringer, Et Al.

2020-Current year OA Pubs

BACKGROUND: Hypothermia is neuroprotective in some ischemia-reperfusion injuries. Ischemia-reperfusion injury may occur with traumatic subdural hematoma (SDH). This study aimed to determine whether early induction and maintenance of hypothermia in patients with acute SDH would lead to decreased ischemia-reperfusion injury and improve global neurologic outcome.

METHODS: This international, multicenter randomized controlled trial enrolled adult patients with SDH requiring evacuation of hematoma within 6 h of injury. The intervention was controlled temperature management of hypothermia to 35 °C prior to dura opening followed by 33 °C for 48 h compared with normothermia (37 °C). Investigators randomly assigned patients at a 1:1 …

Efficacy And Safety Outcomes With Diroximel Fumarate After Switching From Prior Therapies Or Continuing On Drf: Results From The Phase 3 Evolve-Ms-1 Study, Sibyl Wray, Robert T Naismith, Et Al.

2020-Current year OA Pubs

INTRODUCTION: Diroximel fumarate (DRF) is an oral fumarate for relapsing multiple sclerosis (MS) with the same active metabolite as dimethyl fumarate (DMF). DRF has a safety/efficacy profile similar to DMF but with improved gastrointestinal (GI) tolerability and low (< 1%) treatment discontinuation due to GI adverse events (AEs). Efficacy and safety outcomes in patients who switched to DRF from other disease-modifying therapies (DMTs) have not been evaluated.

METHODS: EVOLVE-MS-1 is an ongoing, 2-year, open-label, phase 3 study of DRF in adults with relapsing-remitting MS. Patients either entered as newly enrolled to DRF trials, or from the 5-week, randomized, head-to-head, phase 3 EVOLVE-MS-2 study of DRF and DMF. This analysis evaluated safety and GI tolerability in patients continuing on DRF (DRF-rollover) or switching from DMF (DMF-rollover) following EVOLVE-MS-2. Safety …

First-In-Human, Open-Label, Phase 1/2 Study Of The Monoclonal Antibody Programmed Cell Death Protein-1 (Pd-1) Inhibitor Cetrelimab (Jnj-63723283) In Patients With Advanced Cancers, Enriqueta Felip, Daniel Morgensztern, Et Al.

2020-Current year OA Pubs

PURPOSE: To assess the safety, pharmacokinetics, pharmacodynamics, and preliminary efficacy of cetrelimab (JNJ-63723283), a monoclonal antibody programmed cell death protein-1 (PD-1) inhibitor, in patients with advanced/refractory solid tumors in the phase 1/2 LUC1001 study.

METHODS: In phase 1, patients with advanced solid tumors received intravenous cetrelimab 80, 240, 460, or 800 mg every 2 weeks (Q2W) or 480 mg Q4W. In phase 2, patients with melanoma, non-small-cell lung cancer (NSCLC), and microsatellite instability-high (MSI-H)/DNA mismatch repair-deficient colorectal cancer (CRC) received cetrelimab 240 mg Q2W. Response was assessed Q8W until Week 24 and Q12W thereafter.

RESULTS: In phase 1, 58 patients …

Foxo1 Represses Sprouty 2 And Sprouty 4 Expression To Promote Arterial Specification And Vascular Remodeling In The Mouse Yolk Sac, Nanbing Li-Villarreal, Rebecca Lee Yean Wong, Monica D Garcia, Ryan S Udan, Ross A Poché, Tara L Rasmussen, Alexander M Rhyner, Joshua D Wythe, Mary E Dickinson

Faculty, Staff and Students Publications

Establishing a functional circulatory system is required for post-implantation development during murine embryogenesis. Previous studies in loss-of-function mouse models showed that FOXO1, a Forkhead family transcription factor, is required for yolk sac (YS) vascular remodeling and survival beyond embryonic day (E) 11. Here, we demonstrate that at E8.25, loss of Foxo1 in Tie2-cre expressing cells resulted in increased sprouty 2 (Spry2) and Spry4 expression, reduced arterial gene expression and reduced Kdr (also known as Vegfr2 and Flk1) transcripts without affecting overall endothelial cell identity, survival or proliferation. Using a Dll4-BAC-nlacZ reporter line, we found that one of the earliest expressed …

Targeting Calcium-Mediated Inter-Organellar Crosstalk In Cardiac Diseases, Mohit M Hulsurkar, Satadru K Lahiri, Jason Karch, Meng C Wang, Xander H T Wehrens

Faculty, Staff and Students Publications

INTRODUCTION: Abnormal calcium signaling between organelles such as the sarcoplasmic reticulum (SR), mitochondria and lysosomes is a key feature of heart diseases. Calcium serves as a secondary messenger mediating inter-organellar crosstalk, essential for maintaining the cardiomyocyte function.

AREAS COVERED: This article examines the available literature related to calcium channels and transporters involved in inter-organellar calcium signaling. The SR calcium-release channels ryanodine receptor type-2 (RyR2) and inositol 1,4,5-trisphosphate receptor (IP

EXPERT OPINION: Enhanced SR calcium release via RyR2 and reduced SR reuptake via SERCA2a, increased VDAC and MCUC-mediated calcium uptake into mitochondria, and enhanced lysosomal calcium-release via lysosomal TPC and TRPML …

Prevalence And Predictors Of Missed Dysplasia On Index Barrett's Esophagus Diagnosing Endoscopy In A Veteran Population, Theresa H Nguyen, Aaron P Thrift, Rollin George, Daniel G Rosen, Hashem B El-Serag, Gyanprakash A Ketwaroo

Faculty, Staff and Students Publications

BACKGROUND & AIMS: Limitations of endoscopic sampling may result in missed dysplasia at the diagnosis of Barrett's esophagus (BE). However, the role of close follow-up endoscopy is unclear. The aim was to evaluate the proportion of patients diagnosed with "missed" dysplasia within 18 months of their index nondysplastic BE (NDBE) diagnosis.

METHODS: This was a retrospective analysis of a cohort of BE patients diagnosed during 1990-2019 at the Houston VA. Patients with BE on index esophagogastroduodenoscopy (EGD) were classified as NDBE, indefinite dysplasia, or dysplastic (low- or high-grade dysplasia) based on initial biopsies. We identified NDBE patients who had follow-up …

Colonic Epithelial Angiotensin-Converting Enzyme 2 (Ace2) Expression In Blacks And Whites: Potential Implications For Pathogenesis Covid-19 Racial Disparities, Mart De La Cruz, David P Nunes, Vaishali Bhardwaj, Deepika Subramanyan, Caroline Zaworski, Priya Roy, Hemant K Roy

Faculty, Staff and Students Publications

BACKGROUND: Covid-19 toll is disproportionate in Blacks although the mechanisms remain incompletely understood. From a biological perspective, several host proteins have received most attention as logical susceptibility targets. Specifically, angiotensin-converting enzyme 2 (ACE2) serves as the epithelial cell receptor and acts in concert with transmembrane protease serine 2 (TMPRSS2). Intriguingly, ACE2 can also suppress the inflammatory response and therefore may impact the severity of Covid-19 infections (from the exuberant immune response a.k.a. "cytokine storm"). We, therefore, assessed expression of ACE2 and TMPRSS2 in Blacks versus Whites.

METHODS: Archived mucosal biopsies from colonoscopic biopsies of visually normal rectal mucosa without concurrent …

Centers For Mendelian Genomics: A Decade Of Facilitating Gene Discovery, Samantha M Baxter, Jennifer E Posey, Nicole J Lake, Nara Sobreira, Jessica X Chong, Steven Buyske, Elizabeth E Blue, Lisa H Chadwick, Zeynep H Coban-Akdemir, Kimberly F Doheny, Colleen P Davis, Monkol Lek, Christopher Wellington, Shalini N Jhangiani, Mark Gerstein, Richard A Gibbs, Richard P Lifton, Daniel G Macarthur, Tara C Matise, James R Lupski, David Valle, Michael J Bamshad, Ada Hamosh, Shrikant Mane, Deborah A Nickerson, Heidi L Rehm, Anne O'Donnell-Luria

Faculty, Staff and Student Publications

PURPOSE: Mendelian disease genomic research has undergone a massive transformation over the past decade. With increasing availability of exome and genome sequencing, the role of Mendelian research has expanded beyond data collection, sequencing, and analysis to worldwide data sharing and collaboration.

METHODS: Over the past 10 years, the National Institutes of Health-supported Centers for Mendelian Genomics (CMGs) have played a major role in this research and clinical evolution.

RESULTS: We highlight the cumulative gene discoveries facilitated by the program, biomedical research leveraged by the approach, and the larger impact on the research community. Beyond generating a list of gene-phenotype relationships …

Purpose, Subject, And Consumer Comment On "Perceived Burden Due To Registrations For Quality Monitoring And Improvement In Hospitals: A Mixed Methods Study", Sylvia J Hysong, Patrick O'Mahen, Jochen Profit, Laura A Petersen

Faculty, Staff and Students Publications

Zegers and colleagues’ study codifies the perceived burden of quality monitoring and improvement stemming from the work by clinicians of registering (documenting) quality information in the medical record. We agree with Zegers and colleagues’ recommendation that a smaller, more effective and curated set of measures is needed to reduce burden, confusion, and expense. We further note that focusing on validity of clinical evidence behind individual measures is critical, but insufficient. We therefore extend Zegers and colleagues’ work through a pragmatic, tripartite heuristic. To assess the value of and curate a targeted set of performance measures, we propose concentrating on the …

“One Aims At The Officers First”: Reassessing The Relationship Of C. S. Lewis And T. S. Eliot, Ben Reinhard

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

This article challenges the received account of the relationship between C. S. Lewis and T. S. Eliot, arguing that Lewis's antipathy towards Eliot was a defining feature of his career as a scholar, critic, and literary artist. By attending to the anti-Eliotic strain in Lewis's thought, we gain new insights into the development of his imagination and the creation of his own works, including The Chronicles of Narnia.

Pamela Colman Smith: Tarot Artist: The Pious Pixie By Dawn C. Robinson And Pamela Colman Smith: Artist, Feminist, And Mystic By Elizabeth Foley O'Connor, Emily E. Auger

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Pamela Colman Smith: Tarot Artist: The Pious Pixie. Dawn G. Robinson. Fonthill Media, 2020. 213 pp. with notes, bibliography, and 32 plates. ISBN 978-1-78155-741-9. $29.00.

Pamela Colman Smith: Artist, Feminist, and Mystic. Elizabeth Foley O’Connor. Clemson UP, 2021. 320 pp. with notes. Index, 34 b/w illustrations. Hardcover ISBN 978-1-949979-39-8. $115. eISBN 9781949979404.

Healing Layers Of Trauma: Culturally Responsive Counseling With Asian American Clients, Yun Shi, Atsuko Seto, Sharon Stanley

Counselor Education Faculty Publications and Presentations

Learning Objectives
Objective 1: To understand the wide range of traumatic experiences that affect the Asian American population and implications for counseling practice.
Objective 2: To articulate the foundation for establishing a therapeutic relationship with clients of Asian descent.
Objective 3: To be able to apply 3 5 trauma informed and culturally responsive counseling strategies for facilitating the growth and healing of Asian American clients.

Metabolic Inflexibility In Youth With Obesity: Is It A Feature Of Obesity Or Distinctive Of Youth Who Are Metabolically Unhealthy?, Nour Y Gebara, Joon Young Kim, Fida Bacha, Sojung Lee, Silva Arslanian

Faculty, Staff and Students Publications

Individuals with obesity have metabolic inflexibility with diminished fasting fat oxidation and blunted increase in respiratory quotient (RQ) in insulin-stimulated states. However, it is unclear if metabolic inflexibility is a characteristic of obesity per se or is unique to youth who have metabolically unhealthy obesity (MUO) compared with metabolically healthy obesity (MHO). We investigated metabolic flexibility in youth with MUO, MHO and normal weight (NW) and compared their metabolic characteristics. Youth (n = 188) were divided, based on cut-off points for in vivo insulin sensitivity (IS) of adolescents with NW, into 137 with MUO and 51 with MHO. Fasting hepatic …

Exposure To Per- And Polyfluoroalkyl Substances And Markers Of Liver Injury: A Systematic Review And Meta-Analysis, Elizabeth Costello, Sarah Rock, Nikos Stratakis, Sandrah P Eckel, Douglas I Walker, Damaskini Valvi, Dora Cserbik, Todd Jenkins, Stavra A Xanthakos, Rohit Kohli, Stephanie Sisley, Vasilis Vasiliou, Michele A La Merrill, Hugo Rosen, David V Conti, Rob Mcconnell, Leda Chatzi

Faculty, Staff and Students Publications

BACKGROUND: Experimental evidence indicates that exposure to certain pollutants is associated with liver damage. Per- and polyfluoroalkyl substances (PFAS) are persistent synthetic chemicals widely used in industry and consumer products and bioaccumulate in food webs and human tissues, such as the liver.

OBJECTIVE: The objective of this study was to conduct a systematic review of the literature and meta-analysis evaluating PFAS exposure and evidence of liver injury from rodent and epidemiological studies.

METHODS: PubMed and Embase were searched for all studies from earliest available indexing year through 1 December 2021 using keywords corresponding to PFAS exposure and liver injury. For …

Identification Of An Optimal Dose Of Intravenous Ketamine For Late-Life Treatment-Resistant Depression: A Bayesian Adaptive Randomization Trial, Marijn Lijffijt, Nicholas Murphy, Sidra Iqbal, Charles E Green, Tabish Iqbal, Lee C Chang, Colin N Haile, Lorna C Hirsch, Nithya Ramakrishnan, Dylan A Fall, Alan C Swann, Rayan K Al Jurdi, Sanjay J Mathew

Faculty, Staff and Students Publications

Evidence supporting specific therapies for late-life treatment-resistant depression (LL-TRD) is necessary. This study used Bayesian adaptive randomization to determine the optimal dose for the probability of treatment response (≥50% improvement from baseline on the Montgomery-Åsberg Depression Rating Scale) 7 days after a 40 min intravenous (IV) infusion of ketamine 0.1 mg/kg (KET 0.1), 0.25 mg/kg (KET 0.25), or 0.5 mg/kg (KET 0.5), compared to midazolam 0.03 mg/kg (MID) as an active placebo. The goal of this study was to identify the best dose to carry forward into a larger clinical trial. Response durability at day 28, safety and tolerability, and …

Assessment Of Travel Distance For Hyperthermic Intraperitoneal Chemotherapy In Women With Ovarian Cancer, Laura M Chambers, Meng Yao, Molly Morton, Morgan Gruner, Anna Chichura, Anthony B Costales, Max Horowitz, Peter G Rose, Chad M Michener, Robert Debernardo

Faculty, Staff and Students Publications

OBJECTIVE S: To evaluate travel distance in women with advanced or recurrent epithelial ovarian cancer (OC) undergoing cytoreductive surgery with hyperthermic intraperitoneal chemotherapy (HIPEC) and the subsequent impact upon outcomes.

METHODS: An IRB-approved single-institution prospective registry was queried for women with OC who underwent HIPEC from 1/1/2009-12/1/2020. Demographic, oncologic, and surgical data were recorded. The patient's home zip code was compared to the institutional zip code to determine travel distance using Google Maps. Patients were divided into three strata for analysis: 1) local: ≤50 miles, 2) regional: 51-99 miles, and 3) distant: ≥100 miles and univariate analysis was performed.

RESULTS: …

Retinal Organoids Derived From Rhesus Macaque Ipscs Undergo Accelerated Differentiation Compared To Human Stem Cells, Antonio Jacobo Lopez, Sangbae Kim, Xinye Qian, Jeffrey Rogers, J Timothy Stout, Sara M Thomasy, Anna La Torre, Rui Chen, Ala Moshiri

Faculty, Staff and Students Publications

PURPOSE: To compare the timing and efficiency of the development of Macaca mulatta, a nonhuman primate (NHP), induced pluripotent stem cell (rhiPSC) derived retinal organoids to those derived from human embryonic stem cells (hESCs).

RESULTS: Generation of retinal organoids was achieved from both human and several NHP pluripotent stem cell lines. All rhiPSC lines resulted in retinal differentiation with the formation of optic vesicle-like structures similar to what has been observed in hESC retinal organoids. NHP retinal organoids had laminated structure and were composed of mature retinal cell types including cone and rod photoreceptors. Single-cell RNA sequencing was conducted at …

Intestinal Deletion Of 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Promotes Expansion Of The Resident Stem Cell Compartment, Alexandria M Doerfler, Jun Han, Kelsey E Jarrett, Li Tang, Antrix Jain, Alexander Saltzman, Marco De Giorgi, Marcel Chuecos, Ayrea E Hurley, Ang Li, Pauline Morand, Claudia Ayala, David R Goodlett, Anna Malovannaya, James F Martin, Thomas Q De Aguiar Vallim, Noah Shroyer, William R Lagor

Faculty, Staff and Students Publications

BACKGROUND: The intestine occupies the critical interface between cholesterol absorption and excretion. Surprisingly little is known about the role of de novo cholesterol synthesis in this organ, and its relationship to whole body cholesterol homeostasis. Here, we investigate the physiological importance of this pathway through genetic deletion of the rate-limiting enzyme.

METHODS: Mice lacking 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Hmgcr) in intestinal villus and crypt epithelial cells were generated using a Villin-Cre transgene. Plasma lipids, intestinal morphology, mevalonate pathway metabolites, and gene expression were analyzed.

RESULTS: Mice with intestine-specific loss of Hmgcr were markedly smaller at birth, but gain …

Changes In Physical Activity Associated With The Covid-19 Pandemic In Individuals With Overweight And Obesity: An Interrupted Time Series Analysis With Historical Controls, Michael C Robertson, Che Young Lee, Ivan Haw-Chong Wu, Yue Liao, Margaret Raber, Nathan Parker, Thuan Le, Leticia Gatus, Karen M Basen-Engquist

Faculty, Staff and Students Publications

The objective of this study was to investigate changes in physical activity patterns associated with the COVID-19 pandemic in individuals with overweight and obesity who were participating in a school district worksite weight loss program. We conducted comparative design interrupted time series analyses on physical activity device (Fitbit) data from the 2018-2019 and 2019-2020 school years (N = 211). We administered a questionnaire in 2020 to supplement device data. After the stay-at-home orders in 2020, participants tended to decrease their weekly step count (B = -1315.7, SE = 627.7, p = .045), decrease their weekly "Lightly active minutes" (B = …

Generation Of Crispr-Cas9-Mediated Genetic Knockout Human Intestinal Tissue-Derived Enteroid Lines By Lentivirus Transduction And Single-Cell Cloning, Shih-Ching Lin, Kei Haga, Xi-Lei Zeng, Mary K Estes

Faculty, Staff and Students Publications

Human intestinal tissue-derived enteroids (HIEs; also called organoids) are a powerful ex vivo model for gastrointestinal research. Genetic modification of these nontransformed cultures allows new insights into gene function and biological processes involved in intestinal diseases as well as gastrointestinal and donor segment-specific function. Here we provide a detailed technical pipeline and protocol for using the CRISPR-Cas9 genome editing system to knock out a gene of interest specifically in HIEs by lentiviral transduction and single-cell cloning. This protocol differs from a previously published alternative using electroporation of human colonoids to deliver piggyback transposons or CRISPR-Cas9 constructs, as this protocol uses …