Digital Commons Network^™

Jinetes De La Tormenta: The Invasive Influence Of Gender Constructs On The Journey Towards Selfhood And Societal Progress, Marissa Nicole Luquette

Theses and Dissertations

The novel Jinetes de la tormenta, written by Spanish author, Javier Castañeda de la Torre, presents gender as an obstacle to individuals in the ongoing process of self-discovery (as it relates to personal identity) and as the underlying source of human discontent in the technological age. Jinetes represents gender as a hindrance to the developing self through masculine and feminine binaries in European colonial languages and through the performative behavioral expectations tethered to the gendered body the theoretical works of Jacques Lacan and Hélène Cixous. The theoretical works of Jacques Lacan and Hélène Cixous establish the argument surrounding linguistic gender …

A Cocktail Nanovaccine Targeting Key Entry Glycoproteins Elicits High Neutralizing Antibody Levels Against Ebv Infection, Ling Zhong, Wanlin Zhang, Hong Liu, Xinyu Zhang, Zeyu Yang, Zhenfu Wen, Ling Chen, Haolin Chen, Yanran Luo, Yanhong Chen, Qisheng Feng, Mu-Sheng Zeng, Qinjian Zhao, Lixin Liu, Claude Krummenacher, Yi-Xin Zeng, Yongming Chen, Miao Xu, Xiao Zhang

Faculty, Staff and Student Publications

Epstein-Barr virus (EBV) infects more than 95% of adults worldwide and is closely associated with various malignancies. Considering the complex life cycle of EBV, developing vaccines targeting key entry glycoproteins to elicit robust and durable adaptive immune responses may provide better protection. EBV gHgL-, gB- and gp42-specific antibodies in healthy EBV carriers contributed to sera neutralizing abilities in vitro, indicating that they are potential antigen candidates. To enhance the immunogenicity of these antigens, we formulate three nanovaccines by co-delivering molecular adjuvants (CpG and MPLA) and antigens (gHgL, gB or gp42). These nanovaccines induce robust humoral and cellular responses through efficient …

Women Gaze Back: Affect And Apparatic Disintegration In Funeral Parade Of Roses And Perfect Blue, Jessi Ivie

Theses and Dissertations

This thesis explores how films Funeral Parade of Roses (1969) by Toshio Matsumoto and Perfect Blue (1997) by Satoshi Kon subvert the male gaze of the camera by having the female main characters look back at the audience. The characters Mima (Perfect Blue) and Eddie (Funeral Parade) take control of the camera to subvert the hierarchical relationship between apparatus, audience, and characters. Through the lenses of apparatus and affect theory, these films show how the world of film includes the audience instead of precluding them. Experimental editing and heightened emotional situations unsettle the audience's voyeuristic position, bringing them closer to …

Emerging Themes Regarding Customer Perception Of A Third-Party Online Food Delivery Provider And The Implications For Operators, Shian-Lih Chen Mccain, Jeff Lolli, Emma Liu

Journal of Applied Marketing Theory

This study discusses third-party online food delivery providers' (OFDPs) continuing dominance post-pandemic and provides recommendations for providers and foodservice operators by analyzing customer comments regarding a third-party OFDP. Thematic Analysis, a data-driven inductive approach, was applied in this study to identify four emergent themes. 1) App Technology- Oriented Quality Attributes, 2) App Service-Oriented Quality Attributes, 3) Delivery Person’s Performance, and 4) Restaurant Food Quality. Technology-Oriented Quality Attributes had the highest frequency of mentions (533), followed by Deliverer’s Performance (531), Service-Oriented Quality Attributes (524), and Restaurant Food Quality (115) for a total of 1,703 mentions of which 461(27%) were positive and …

Differences In Art And Music High School Students’ Sat Test Scores From 2006 To 2015, Margaret Ellen Beyer

Walden Dissertations and Doctoral Studies

Academia and education advocates have reported a concern about the elimination of art and music education classes from high school curriculums in the United States. Studies using mean SAT test scores from 1984 and 1994 versions of the SAT test indicated participants who had art and music education classes had higher verbal and mathematical SAT test scores compared to those who did not. However, since revision of the SAT test in 2005, whether students had significantly increased mean SAT test scores with each added year of art and music education participation, and whether students who participated in art and music …

Rotational Rheometry Test Of Portland Cement-Based Materials – A Systematic Literature Review, Laura Silvestro, Artur Spat Ruviaro, Geannina Lima, Luís Urbano Durlo Tambara Júnior, Dimitri Feys, Ana Paula Kirchheim

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

This Study Systematically Reviews 62 Papers on the Use of Rotational Rheometry to Assess the Fresh State Behavior of Portland Cement-Based Materials. the Research Highlights the Wide Variation in Test Methods and Aims to Provide a Comprehensive overview. Findings Reveal that 50.0% of Studies Employed Vane Geometry, despite its Limitations in Providing Transformation Equations. Regarding Dynamic Shearing Tests, 67.0% Followed a Consensus using a Pre-Shearing Step and a Stepwise Routine with Stabilization Times ≥ 10 S. While the Bingham Model is Commonly Used, the Study Emphasizes the Importance of Considering Shear-Thinning Behavior in Cementitious Materials. Models Like Herschel-Bulkley and Modified …

Improving The Language Competencies Of Non-Native Arabic Speakers Through The Communicative Approach, Ahmad Aljanadbah, Ali Al Sharef

All Works

There is no doubt that the communicative approach is one of the most important methodologies in teaching foreign languages to non-native speakers. Therefore, learners are required to use the language as efficiently as a native speaker. This research thoroughly examines the unique attributes of the communicative approach and offers insights into its effective implementation within the classroom setting. The foundation of this study lies in understanding the functional aspects of language and its practical application in real-world contexts. It explores the methodology for teaching communicative Arabic language skills to non-native speakers. Additionally, the research explores the concept of bridging the …

Development Of An Online Tool To Support Financial And Legal Planning In Dementia, Irene Tessaro, Sarah M. Hooper, Denise Watt, David Menestres, David Farrell

Faculty Scholarship

Objective: To develop, assess, and refine an online educational tool, Plan for Clarity, to support financial and legal planning in dementia.

Methods: A Delphi mixed-method study with three rounds of anonymous review by lay and professional stakeholders was designed to reach consensus about the content of the online tool and explore the socio-cultural and behavioral factors that could affect access and use.

Results: Consensus showed that the online tool covered key information, knowledge, and communication skills for financial and legal planning. Study themes: 1) the online tool had to be easy to navigate with relevant, easily understood …

The Case(S) Of Environmental And Sustainability Education In The United Kingdom, Alun Morgan, Paul Warwick

School of Law, Humanities and Social Sciences

The story of Environmental and Sustainability Education (ESE) in the United Kingdom (UK) is manifold in nature – with multiple initiatives interacting in the ebb and flow of its progress. The UK remains dis-united at an educational policy level, with Scotland, Wales and Northern Ireland leading the way in their recent emphasis on the importance of ESE in comparison to England.The exciting potential for ESE change has historically been more consistently harnessed across the UK through the Third Sector helping to catalyse change across the public Education Sector. Charities, voluntary and community organisations, social enterprises and cooperatives have partnered with …

“She’S Been A Rock”: The Function And Importance Of “Holding” By Social Prescribing Link Workers In Primary Care In England—Findings From A Realist Evaluation, Debra Westlake, Geoffrey Wong, Steven Markham, Amadea Turk, Jordan Gorenberg, Catherine Pope, Joanne Reeve, Caroline Mitchell, Kerryn Husk, Sabi Redwood, Anthony Meacock, Kamal R. Mahtani, Stephanie Tierney

Peninsula Medical School

Social prescribing link workers are recently introduced roles in English primary care. One of their intended functions is to support patients with conditions influenced by the wider, social determinants of health. Their main purpose is to connect people to community resources to meet their nonmedical needs. However, our research reveals that link workers provide not only connections but also what we have described as “holding” for individuals with complex needs, who lack informal networks of support or who are waiting to access services. We explore the concept of holding, its meaning and significance in this context, and consider its consequences. …

Initiation Of Hnrnpa1 Low-Complexity Domain Condensation Monitored By Dynamic Light Scattering, Phoebe S Tsoi, Josephine C Ferreon, Allan Chris M Ferreon

Faculty, Staff and Students Publications

Biomolecular condensates (BMCs) exhibit physiological and pathological relevance in biological systems. Both liquid and solid condensates play significant roles in the spatiotemporal regulation and organization of macromolecules and their biological activities. Some pathological solid condensates, such as Lewy Bodies and other fibrillar aggregates, have been hypothesized to originate from liquid condensates. With the prevalence of BMCs having functional and dysfunctional roles, it is imperative to understand the mechanism of biomolecular condensate formation and initiation. Using the low-complexity domain (LCD) of heterogenous ribonuclear protein A1 (hnRNPA1) as our model, we monitored initial assembly events using dynamic light scattering (DLS) while modulating …

Identifying Interventions To Improve Diagnostic Safety In Emergency Departments: Protocol For A Participatory Design Study, Woosuk Seo, Sun Young Park, Zhan Zhang, Hardeep Singh, Kalyan Pasupathy, Prashant Mahajan

Faculty, Staff and Students Publications

Background: Emergency departments (EDs) are complex and fast-paced clinical settings where a diagnosis is made in a time-, information-, and resource-constrained context. Thus, it is predisposed to suboptimal diagnostic outcomes, leading to errors and subsequent patient harm. Arriving at a timely and accurate diagnosis is an activity that occurs after an effective collaboration between the patient or caregiver and the clinical team within the ED. Interventions such as novel sociotechnical solutions are needed to mitigate errors and risks.

Objective: This study aims to identify challenges that frontline ED health care providers and patients face in the ED diagnostic process and …

Analyzing Efficiency Of A Lentiviral Shrna Knockdown System In Human Enteroids Using Western Blot And Flow Cytometry, Adam P Wilson, Karni S Moshal, Addison P Franca, Sasirekha Ramani, Randle Gallucci, Hala Chaaban, Kathryn Y Burge

Faculty, Staff and Students Publications

Enteroids are in vitro models to study gastrointestinal pathologies and test personalized therapeutics; however, the inherent complexity of enteroids often renders standard gene editing approaches ineffective. Here, we introduce a refined lentiviral transfection protocol, ensuring sufficient lentiviral engagement with enteroids while considering spatiotemporal growth variability throughout the extracellular matrix. Additionally, we highlight a selection process for transduced cells, introduce a protocol to accurately measure transduction efficiency, and explore methodologies to gauge effects of gene knockdown on biological processes.

Protocol For Preclinical Evaluation Of Locoregionally Delivered Car T Cells In Patient-Derived Xenograft Models Of Brain Tumors, Benjamin Draper, Chantelle Bowers, Eleni Vassalou, Ailsa Greppi, John Anderson, Nabil Ahmed, Michael D Taylor, Laura K Donovan

Faculty, Staff and Students Publications

Here, we present a protocol for preclinical evaluation of locoregionally delivered CAR T cells in patient-derived xenograft models of primary, metastatic, and recurrent brain tumors. We provide instructions for isolating peripheral blood mononuclear cells (PBMCs), producing CAR T cells in conjunction with locoregional delivery, and preclinical trial design and analysis involving CAR T cells. Additionally, we describe comprehensive preclinical readouts and guidelines for critical endpoint sample collections. In line with clinical trial procedures, our protocol broadens available treatment modalities for direct clinical translation. For complete details on the use and execution of this protocol, please refer to Donovan et al …

Esr1 And P53 Interactome Alteration Defines Mechanisms Of Tamoxifen Response In Luminal Breast Cancer, Chetan C Oturkar, Spencer R Rosario, Alan D Hutson, Adrianne Groman, Stephen B Edge, Carl D Morrison, Wendy M Swetzig, Jianmin Wang, Jun Hyoung Park, Benny Abraham Kaipparettu, Prashant K Singh, Shicha Kumar, Helen H Cappuccino, Manish Ranjan, Araba Adjei, Mohammad Ghasemi, Andrew K L Goey, Swati Kulkarni, Gokul M Das

Faculty, Staff and Students Publications

The canonical mechanism behind tamoxifen's therapeutic effect on estrogen receptor α/ESR1+ breast cancers is inhibition of ESR1-dependent estrogen signaling. Although ESR1+ tumors expressing wild-type p53 were reported to be more responsive to tamoxifen (Tam) therapy, p53 has not been factored into choice of this therapy and the mechanism underlying the role of p53 in Tam response remains unclear. In a window-of-opportunity trial on patients with newly diagnosed stage I-III ESR1+/HER2/wild-type p53 breast cancer who were randomized to arms with or without Tam prior to surgery, we reveal that the ESR1-p53 interaction in tumors was inhibited by Tam. This resulted in …

Comprehensive Single-Cell Atlas Of The Mouse Retina, Jin Li, Jongsu Choi, Xuesen Cheng, Justin Ma, Shahil Pema, Joshua R Sanes, Graeme Mardon, Benjamin J Frankfort, Nicholas M Tran, Yumei Li, Rui Chen

Faculty, Staff and Students Publications

Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of cellular heterogeneity by characterizing cell types across tissues and species. While several mouse retinal scRNA-seq datasets exist, each dataset is either limited in cell numbers or focused on specific cell classes, thereby hindering comprehensive gene expression analysis across all retina types. To fill the gap, we generated the largest retinal scRNA-seq dataset to date, comprising approximately 190,000 single cells from C57BL/6J mouse retinas, enriched for rare population cells via antibody-based magnetic cell sorting. Integrating this dataset with public datasets, we constructed the Mouse Retina Cell Atlas (MRCA) for wild-type mice, encompassing …

Protocol For Optical, Aqueous-Based Clearing Of Murine Tissues Using Ez Clear, Taeyong Ahn, Gabrielle E Largoza, Julia Younis, Mary E Dickinson, Chih-Wei Hsu, Joshua D Wythe

Faculty, Staff and Students Publications

Tissue clearing is an essential prerequisite for 3D volumetric imaging of larger tissues or organs. Here, we present a detailed protocol for optical, aqueous-based clearing of adult murine tissues using EZ Clear. We describe steps to ensure successful perfusion and fixation of organs from the adult mouse and supply guidelines for optimal lipid removal, refractive index matching, and tissue clearing. Finally, we provide imaging parameters for visualizing both exogenous perfused fluorescent dyes and endogenous fluorescence reporters in the adult mouse. For complete details on the use and execution of this protocol, please refer to Hsu et al.

Study Of Prognostic Splicing Factors In Cancer Using Machine Learning Approaches, Mengyuan Yang, Jiajia Liu, Pora Kim, Xiaobo Zhou

Faculty, Staff and Student Publications

Splicing factors (SFs) are the major RNA-binding proteins (RBPs) and key molecules that regulate the splicing of mRNA molecules through binding to mRNAs. The expression of splicing factors is frequently deregulated in different cancer types, causing the generation of oncogenic proteins involved in cancer hallmarks. In this study, we investigated the genes that encode RNA-binding proteins and identified potential splicing factors that contribute to the aberrant splicing applying a random forest classification model. The result suggested 56 splicing factors were related to the prognosis of 13 cancers, two SF complexes in liver hepatocellular carcinoma, and one SF complex in esophageal …

Alzheimer's Disease Risk Gene Cd2ap Is A Dose-Sensitive Determinant Of Synaptic Structure And Plasticity, Taeyong Ahn, Gabrielle E Largoza, Julia Younis, Mary E Dickinson, Chih-Wei Hsu, Joshua D Wythe

Faculty, Staff and Students Publications

Tissue clearing is an essential prerequisite for 3D volumetric imaging of larger tissues or organs. Here, we present a detailed protocol for optical, aqueous-based clearing of adult murine tissues using EZ Clear. We describe steps to ensure successful perfusion and fixation of organs from the adult mouse and supply guidelines for optimal lipid removal, refractive index matching, and tissue clearing. Finally, we provide imaging parameters for visualizing both exogenous perfused fluorescent dyes and endogenous fluorescence reporters in the adult mouse. For complete details on the use and execution of this protocol, please refer to Hsu et al.

Book Review Of Drawn Together By Minh Lê And Dan Santat, Simon Boonsripaisal

Journal of Southeast Asian American Education and Advancement

Drawn Together, written by Minh Lê and illustrated by Dan Santat, depicts the story of a grandson and grandfather spending time in the afternoon together. Lê and Santat’s shared experiences and cultures influence the picturebook’s construction. The greatest strength in Drawn Together is the inclusion of family in the creation process. Lê’s meaningful words and phrases complement Santat’s colorful and energetic illustrations. The setting is in the grandfather’s house and away from the site of Thai restaurants and Thai Buddhist temples, to which most of the existing research on Thai Americans hones it on (Bao, 2015; Padoongpatt, 2017). Drawn Together …

Vocational Formation, Belonging And Christian Higher Education, Christopher W. Cobbler Sr.

Tapestry: Journal of Research in Education

Vocational formation and belonging are complimentary concepts. Vocational formation seeks human flourishing by attending to the healing of our social environments. Vocational formation is responding to God’s invitation to participate in the healing of creation from a particular social context, with others, for the sake of the world. Belonging, then, is the natural consequence of such an endeavor. This paper assumes the notion of institutional belonging in Christian higher education to be a kingdom characteristic that is best realized through the various postures of vocational formation. Radical hospitality, missional eschatology and communion with God are among the distinctive qualities of …

Nitrogen In Stubble As A Limiting Factor Of Regrowth And Yield Of Orchardgrass After Cutting, T Matsunaka, T Ishii, M Jin

IGC Proceedings (1977-2023)

Two field trials were carried out to reexamine the role of total nonstructural carbohydrates (TNC) and nitrogen (N) of the stubble in regrowth of orchardgrass (Dactylis Glomerata L.) during 10 days after cutting and the yield of next cutting. The regrowth and the yield were independent of TNC content in the stubble. They increased with N content in the stubble derived from the different rate of previous N application. When the TNC content in the stubble at the time of cutting was more than about 18% (dry matter basis), the regrowth even in a darkroom was not affected by …

Mission (Command) Complete: Implications Of Jadc2, Joseph M. Mcgiffin

Joint Force Quarterly

Command and control (C2) is crucial in military operations, guiding how authority directs missions. As warfare evolves with AI, hypersonic weapons, and autonomous platforms, the U.S. military is advancing its Joint All-Domain Command and Control (JADC) initiatives. This effort aims to unify military networks for better decision-making. This article explores challenges in adapting Joint C2 networks for JADC2. It compares new technologies with current military practices, highlighting where changes are needed. Key areas include strategic planning, tactical operations, and how JADC2 affects mission command. Recommendations focus on improving readiness for future conflicts by enhancing interoperability and decision-making across military branches.

The Other Arctic: Competition, Cooperation, Or Coexistence?, John B. Kelley, Christopher J. Sarton, Scott A. Curtice, Charles C. York Iii

Joint Force Quarterly

In Antarctica, the United States must mitigate risks stemming from competitive influences working to alter the current paradigms, but it also should seize the opportunities that Antarctica’s unique status affords. As climate change continues to open new opportunities in polar regions, international concern has grown over the potential for the future irresponsible use of the area by actors more concerned with military and economic advantage than conservation efforts. Apprehension over the People’s Republic of China (PRC)’s activities raises more significant concerns that Antarctica may become a region of military competition instead of global cooperation. This apprehension is well-founded but not …

The Impact Of Texas High School Teachers' Perceptions Of Culture On Their Intent To Stay Or Leave From Their Position, Abigail Crawford, Lesley F. Leach, Ron Rhone, Juanita Reyes

School Leadership Review

ABSTRACT

Teacher attrition has been an issue in education for decades, however, post-pandemic, the problem has grown exponentially. Teachers at the high school campus are often particularly difficult to replace because of their specialized areas. Yearly faculty surveys often ask teachers to share their perceptions of their campus, including but not limited to, faculty relationships, administrative decisions, student discipline, and learning and assessment. While teachers’ perceptions of these areas of campus life might be positive or negative, do any of these areas motivate a teacher to leave or to stay? The purpose of this research is to investigate high school …

Bilingual Education Program Type And The Reading Performance Of Emergent Bilingual Children: A Multiyear, Texas Investigation, Clare A. Resilla, John R. Slate

School Leadership Review

In this study, we examined the extent to which Grade 3 reading performance of Emergent Bilingual students differed by bilingual education program type (i.e., Dual Language Two-Way, Dual Language One-Way, Transitional Early Exit, Transitional Late Exit). We specifically analyzed data for three consecutive years (i.e., 2016-2017, 2017-2018, and 2018-2019) of data about their performance in the three reading reporting categories (i.e., Category 1, Category 2, and Category 3) and the three grade level performance measures (i.e., Approaches Grade Level, Meets Grade Level, and Masters Grade Level) before the pandemic. In all 18 analyses, Emergent Bilingual students in the Dual Language …

Tutors’ Experiences Of Using A Data-Free Mobile Instant Messenger For Electronic Tutoring: A Case Study In Accounting And Information Systems Courses, Fazlyn Petersen, Ronald Arendse

The African Journal of Information Systems

In South Africa, data-intensive synchronous tutoring platforms like Zoom can be exclusionary due to limited internet access at home. Challenges in electronic tutoring include device scarcity, high data costs, network issues and low digital skills. To mitigate data costs, a data-free mobile instant messenger was tested for tutoring, guided by the technological pedagogical content knowledge framework (TPACK). The study included first-year accounting and third-year information systems courses at a historically disadvantaged institution. Qualitative data from tutors revealed that technology-related knowledge was crucial for effective electronic tutoring. Tutors with strong technology and content knowledge utilised the data-free application effectively and employed …

Crt: Empowering Students Through Inclusive Education, Jason Caballero

Graduate Teacher Education

Culturally relevant teaching has emerged as a notable teaching approach in the evolving educational landscape, aiming to integrate students' cultural backgrounds into the learning process for a more inclusive classroom environment. Unlike superficial gestures, CRT addresses systemic issues within education by fostering cultural consciousness and appreciation. By affirming cultural identities and promoting mutual respect among students and teachers, CRT strengthens the classroom fabric, enhancing student learning experiences. Understanding the pivotal role of cultural competence in shaping the school climate and equipping educators with the tools and resources necessary to implement CRT effectively is essential. Through targeted training initiatives and comprehensive …

Successful Management Of Chromoblastomycosis Utilizing Conventional Antifungal Agents And Imiquimod Therapy, Jinjin Zheng, Shougang Liu, Zhenmou Xie, Yangxia Chen, Liyan Xi, Hongfang Liu, Yinghui Liu

Faculty, Staff and Student Publications

Chromoblastomycosis (CBM), a chronic fungal infection affecting the skin and subcutaneous tissues, is predominantly caused by dematiaceous fungi in tropical and subtropical areas. Characteristically, CBM presents as plaques and nodules, often leading to scarring post-healing. Besides traditional diagnostic methods such as fungal microscopy, culture, and histopathology, dermatoscopy and reflectance confocal microscopy can aid in diagnosis. The treatment of CBM is an extended and protracted process. Imiquimod, acting as an immune response modifier, boosts the host's immune response against CBM, and controls scar hyperplasia, thereby reducing the treatment duration. We present a case of CBM in Guangdong with characteristic reflectance confocal …

Multimodal Transportation Planning, Chen Peng, Tia Boyd, Kristine Williams

Mavs Open Press Open Educational Resources - Archive

OERTransport Multimodal Transportation Planning introduces readers to the principles and practice of integrated land use and transportation planning. The primary focus of the textbook is on planning for the mobility needs of all transportation system users, with an emphasis on pedestrian, bicycle, automobile, and transit modes while reducing society’s dependence on the automobile for personal travel.

Providing individuals with a choice of clean, efficient, and affordable mobility options is a key strategy for reducing the effects of climate change, improving public health and safety, and achieving more equitable and livable communities. To address these policy objectives, students are expected to …