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Articles 28411 - 28440 of 1326986
Full-Text Articles in Entire DC Network
Holistic Approaches To Lyme Disease Symptom Management, Leah Van Antwerp
Holistic Approaches To Lyme Disease Symptom Management, Leah Van Antwerp
Honors Theses
Lyme disease is a multisystem tick-borne infection that can lead to long-lasting symptoms even after standard antibiotic therapy, with Lyme arthritis being one of the most common and persistent complications. Due to limited research on this issue, rheumatoid arthritis (RA) will be discussed as a reference for understanding treatment options because it is a similar condition. Since many people continue to experience debilitating pain and reduced quality of life after treatment for Lyme disease, holistic approaches have become increasingly popular for symptom management. This literature review examines three holistic modalities-yoga, acupuncture, and diet modifications- and their effectiveness in alleviating arthritic …
Semantic Networks In Relapsing-Remitting Multiple Sclerosis Exhibit Reduced Efficiency, Interconnectivity, And Flexibility, Sophia Lall, Jennifer Pardo, Joshua Sandry
Semantic Networks In Relapsing-Remitting Multiple Sclerosis Exhibit Reduced Efficiency, Interconnectivity, And Flexibility, Sophia Lall, Jennifer Pardo, Joshua Sandry
Department of Psychology Faculty Scholarship and Creative Works
Background
Word-finding deficits are common in persons with relapsing-remitting multiple sclerosis (pwRRMS); this may be related to the inefficient organization of semantic information.
Objective
To understand whether the semantic organization and semantic retrieval are impacted in pwRRMS.
Methods
Semantic fluency data from 64 pwRRMS and 73 controls was utilized to (1) derive standard verbal fluency measures using the Semantic Network and Fluency Utility R package and (2) build semantic networks via the correlation-based network approach in the SemNet R package. Subjective word-finding concerns were assessed in a subgroup of the sample. Group differences were evaluated.
Results
PwRRMS endorsed more frequent …
The Good Life And Human Flourishing: Timeless Principles From Aristotle To Tolstoy, Daniel Mark Kelly
The Good Life And Human Flourishing: Timeless Principles From Aristotle To Tolstoy, Daniel Mark Kelly
Honors Capstone Projects and Theses
No abstract provided.
Gambling Behaviors, Perceptions, And Risks Among Nevada Adults, Andrea Dassopoulos
Gambling Behaviors, Perceptions, And Risks Among Nevada Adults, Andrea Dassopoulos
International Gaming Institute Faculty Research
This study presents a statewide assessment of gambling behaviors, perceptions, and gambling-related risk among 1,078 Nevada adults. Using a mixed-source, population-calibrated survey developed by UNLV and NORC, the research measures gambling participation, motivations, venue exposure, and attitudes toward harm, treatment, and regulation. The Problem Gambling Severity Index (PGSI) identifies substantial levels of harm: nearly half of past-year gamblers show some degree of risk, with the highest rates among younger adults, racially diverse groups, gaming and hospitality workers, and those who gamble in neighborhood bars or slot parlors. The survey also documents public beliefs about gambling addiction, youth exposure, stigma, and …
Influence Of Lunar Light On Vertical Distributions Of Myctophid, Hygophum Taaningi, Meta Hughes
Influence Of Lunar Light On Vertical Distributions Of Myctophid, Hygophum Taaningi, Meta Hughes
All HCAS Student Capstones, Theses, and Dissertations
Hygophum taaningi (Myctophidae) likely undertakes lunar vertical migration (LVM), an asynchronous migration, but there is a lack of literature supporting this behavior in the northern Gulf of Mexico basin. The aim of this study was to assess how effective modeled lunar phase and lunar light intensity are at predicting standardized counts of H. taaningi in the northern Gulf. The R package moonlit was used to provide retroactive calculations of lunar phase and lunar light intensity for standardized count data from the Offshore Nekton Sampling and Analysis Program (ONSAP). A generalized additive model (GAM) was used to investigate the relationship between …
Inhibitory Control, Attentional Bias, And Palatable Food Consumption In Adolescents: A Laboratory Feasibility Randomized Controlled Trial., Carolina M. Bejarano, Adrian Ortega, Christopher C. Cushing
Inhibitory Control, Attentional Bias, And Palatable Food Consumption In Adolescents: A Laboratory Feasibility Randomized Controlled Trial., Carolina M. Bejarano, Adrian Ortega, Christopher C. Cushing
Manuscripts, Articles, Book Chapters and Other Papers
BACKGROUND: Understanding relationships among inhibitory control, attentional bias to food cues, and food consumption in nonclinical adolescent samples can inform preventive efforts for disordered eating and associated health risks.
OBJECTIVE: This pilot study conducted preliminary testing of an inhibitory control training intervention on attentional bias toward food cues, hedonic appetite, and food consumption, as well as examined inhibitory control's relationship with hedonic appetite, food consumption, binge eating, and cognitive restraint.
METHODS: Participants (N=43; mean age 15.1, SD 1.7 years; 31/43, 72% female) were randomized to a food cue-specific go/no go inhibitory control training intervention or control group, took part in …
Reimagining Pediatric Care: The Future Of Telehealth, Mike Smith, Morgan Waller
Reimagining Pediatric Care: The Future Of Telehealth, Mike Smith, Morgan Waller
Pediatrics in Practice: A CME Podcast
Telehealth has transformed the way families access pediatric care, offering convenience without compromising quality. In this episode, we sit down with Morgan Waller, Director of Telemedicine at Children’s Mercy, to explore the latest advancements in pediatric telehealth. From innovative technologies to strategies for improving access in rural communities, we discuss how virtual care is shaping the future of pediatrics and what it means for families and providers alike.
Click here to stream the podcast episode.
Welcoming Spaces: Parent Leadership And Early Literacy As Community-Based Co-Regulation Supports, Lauren S. Dake
Welcoming Spaces: Parent Leadership And Early Literacy As Community-Based Co-Regulation Supports, Lauren S. Dake
Masters Projects
Many young children exhibit challenging behavior in early childhood settings, often as a response to high levels of stress in their home and community environments. This applied master’s project presents Welcoming Spaces, a parent-led initiative developed to support children’s emotional development, caregiver well-being, and early literacy through informal, community-based experiences. In response to concerns about rising dysregulation and reduced access to supportive environments, the project offers portable play areas hosted by parents at events such as school functions, library gatherings, and local festivals. These spaces include calming sensory activities and opportunities for shared reading, while also providing caregivers with …
2024 Child Fatality Annual Report, Tennessee. Department Of Health.
2024 Child Fatality Annual Report, Tennessee. Department Of Health.
Child Fatalities in Tennessee Report
No abstract provided.
Evaluating Remotely Sensed Spectral Indices To Quantify Seagrass In Support Of Ecosystem-Based Fisheries Management In A Marine Protected Area Of Western Australia, Nick Konzewitsch, Lara Mist, Scott N. Evans
Evaluating Remotely Sensed Spectral Indices To Quantify Seagrass In Support Of Ecosystem-Based Fisheries Management In A Marine Protected Area Of Western Australia, Nick Konzewitsch, Lara Mist, Scott N. Evans
Fisheries Research Articles
Highlights
What are the main findings?- Four spectral indices were identified as important for the quantification of seagrass within and adjacent to the MSC-certified Western Australia Enhanced Greenlip Abalone Fishery. The Normalised Difference Aquatic Vegetation Index (NDAVI) and Depth Invariant Index of the blue and green bands were the most important indices.
- Similar seagrass cover and distribution were observed inside and outside of the fishery area of operation.
- The use of indices from free satellite products via Google Earth Engine workflows and automatic image annotation provides a rapidly repeatable method to support …
U-Glide Program Fall 2025, Unf Office Of Undergraduate Research, Unf Graduate School
U-Glide Program Fall 2025, Unf Office Of Undergraduate Research, Unf Graduate School
Undergraduate – Graduate Learning, Inquiry, and Distinctive Experimentation (U-GLIDE) Symposium Programs
No abstract provided.
A Literture Review Of Relative Pose Estimation Using Visual Data, Jamie Jarut Roberson
A Literture Review Of Relative Pose Estimation Using Visual Data, Jamie Jarut Roberson
Honors Capstone Projects and Theses
No abstract provided.
Outcomes After Ulnar Collateral Ligament Reconstruction And Repair In Nonthrowing Athletes, William L. Johns, Neel K. Patel, Benjamin Miltenberg, Ryan W. Paul, Adeeb J. Hanna, Michael C. Ciccotti, Steven B. Cohen, Michael G. Ciccotti, Christopher C. Dodson
Outcomes After Ulnar Collateral Ligament Reconstruction And Repair In Nonthrowing Athletes, William L. Johns, Neel K. Patel, Benjamin Miltenberg, Ryan W. Paul, Adeeb J. Hanna, Michael C. Ciccotti, Steven B. Cohen, Michael G. Ciccotti, Christopher C. Dodson
Rothman Institute Papers
BACKGROUND: Surgical management of ulnar collateral ligament (UCL) tears in nonthrowing athletes is less commonly performed, and the outcomes of that management have not been thoroughly described.
PURPOSE: To describe the clinical outcomes of surgical management of UCL injury in nonthrowing athletes.
STUDY DESIGN: Case series; Level of evidence, 4.
METHODS: A retrospective review was conducted on all patients who underwent UCL reconstruction or repair across a 10-year period (2010-2020), with nonthrowing athletes who had >2-year follow-up being specifically identified. Demographic variables such as age, sport participation, concomitant procedures, and graft type, as well as postoperative outcomes such as complications, …
Hellmuth Owners Corp. V. Kaplan
Hellmuth Owners Corp. V. Kaplan
All Decisions
In this nonpayment proceeding for a co-op, the landlord moved to dismiss tenant's counterclaims and affirmative defenses, and the tenant cross-moved for discovery. The court largely granted the landlord's motion, severing many counterclaims (including monetary damages, injunctive, and declaratory relief) without prejudice due to Housing Court's limited subject matter jurisdiction. Several affirmative defenses were also dismissed without prejudice for being insufficiently pleaded or devoid of merit. However, the tenant's constructive eviction counterclaim was allowed to proceed. The tenant's motion for discovery was denied as overly broad.
A Virtual Or In-Person Infrared Spectroscopy Laboratory Activity For The Analysis Of Fibers And Understanding Of Spectral Library Searches, Erika L. Doctor, Alanna L. Lecher
A Virtual Or In-Person Infrared Spectroscopy Laboratory Activity For The Analysis Of Fibers And Understanding Of Spectral Library Searches, Erika L. Doctor, Alanna L. Lecher
Faculty and Staff Publications & Presentations
The COVID-19 pandemic caused a rapid pivot of instruction in higher education from traditionally in-person modes to virtual modes with a need to maintain student learning outcomes, especially in laboratory courses. The next pandemic and other natural disasters cannot be predicted, and there are other reasons such as lack of resources that may inhibit courses from being implemented in-person. This paper provides a laboratory activity that can be completed in-person or with virtual instruction. This laboratory activity provides a novel approach to teaching students about fiber analysis using ATR-FTIR and elucidates the process of spectral analysis with databases. Students use …
Early Warning Detection Of Gravitational Waves With Sgnl, Megan Norris
Early Warning Detection Of Gravitational Waves With Sgnl, Megan Norris
Honors Theses
Gravitational waves (GWs) provide a whole new spectrum with which to observe astrophysical events. Multi-messenger observations including GWs are of particular interest, and these are best facilitated by early warning searches of GWs, which are typically aimed at detecting binary neutron star (BNS) mergers. This work provides an introductory overview of gravitational waves and summarizes the origins and basic physical properties of neutron stars and black holes in binary systems, focusing on BNS mergers and the detection of gravitational waves with LIGO using a modeled search. We assess the performance of a new GW search pipeline called SGNL to evaluate …
Teaching With V.I.S.I.O.N.: A Qualitative Case Study, Abbrissola A. Shull
Teaching With V.I.S.I.O.N.: A Qualitative Case Study, Abbrissola A. Shull
Graduate Theses, Dissertations, and Capstones
This qualitative case study explored how those matriculating through a teacher education program can develop a critical consciousness of K-12 students experiencing homelessness or high mobility using the Teaching with V.I.S.I.O.N workshop model. This acronym stands for V: Value the culture and lived experiences of students reflected in the classroom, I: Implicit Bias Awareness, S: Study scenarios using the equity literacy case analysis approach (Gorski, 2018), I: Increase the diversity of texts provided in the classroom to reflect these experiences, O: On-going reflection regarding current practices and ways to improve using equity literacy (Gorski, 2018), …
Pregnancy Intention And Maternal Morbidity In Oklahoma And Mississippi: A Moderation Analysis Of Abortion Restriction Policies, Vicki Johnson
Pregnancy Intention And Maternal Morbidity In Oklahoma And Mississippi: A Moderation Analysis Of Abortion Restriction Policies, Vicki Johnson
Walden Dissertations and Doctoral Studies
More than half of all pregnancies are unintended (mistimed or unwanted), and women with unplanned pregnancies may face economic hardships for families and additional health consequences if they have pre-existing medical conditions. This quantitative study was conducted to examine the relationship between pregnancy intention levels and severe acute maternal morbidity, specifically chorioamnionitis, among women who gave birth in Oklahoma and Mississippi in 2022. Using secondary data (N=1939) from the Pregnancy Risk Assessment Monitoring System Automated Research Files and state-level abortion policy data from the Guttmacher Institute, this study employed Bronfenbrenner’s social ecological model framework to investigate the relationship between pregnancy …
Critically Thinking Through A Multiple-Patient Simulation: Applying Real World Principles, Danielle E. Ely, Becky Fights, Allison Johnson, Rachelle Fuller, Karrie Osborne, Jane Milleman, Pamela K. Brelage
Critically Thinking Through A Multiple-Patient Simulation: Applying Real World Principles, Danielle E. Ely, Becky Fights, Allison Johnson, Rachelle Fuller, Karrie Osborne, Jane Milleman, Pamela K. Brelage
Biennial Convention (CONV)
Background Simulating multiple-patient care scenarios enables nursing students to put prioritization and time-management into practice prior to working as registered nurses in the healthcare environment. This project aimed to implement and evaluate a multi-patient simulation.
Method Senior nursing students enrolled in leadership and management experience a multi-patient simulation in which learners are assigned various roles to support four diverse patients. Students exercise autonomy in problem solving as the patient situations unfold around them. Students navigate admissions, transfers, discharges, patient noncompliance, medical complications, and complex family dynamics. After participation, students completed an evaluation of the experience.
Results After approval from the …
What Is The Hospital Pharmaceutical Care? Perceptions And Expectations Of Doctors In Interprofessional Collaboration In 2 Types Of Indonesia Hospitals, Anisyah Achmad, Savvy Augustin Tirta, Titi Annisa Bella, Ratna Kurnia Illahi, Bambang Sidharta, Valentina Yurina, Wara Rejeki
What Is The Hospital Pharmaceutical Care? Perceptions And Expectations Of Doctors In Interprofessional Collaboration In 2 Types Of Indonesia Hospitals, Anisyah Achmad, Savvy Augustin Tirta, Titi Annisa Bella, Ratna Kurnia Illahi, Bambang Sidharta, Valentina Yurina, Wara Rejeki
Pharmacology and Clinical Pharmacy Research
Hospital Pharmaceutical Care (HPC) is the pharmacist’s direct responsibility to aim for patient safety and quality of life. HPC is a means of avoiding medication and human errors. Carrying out a HPC requires a good collaboration between doctors and pharmacists in treatment management. The aim of the research was to determine the relationship between doctors’ perceptions and expectations of clinical pharmacists at HPC. This research is descriptive quantitative observational with a cross-sectional approach. The data were collected using purposive sampling (type A hospital) and total sampling (type C hospital) through interviews using perception and expectations questionnaires on doctors’ to pharmacists …
Effective Strategies To Attract And Retain Qualified Hospitality Staff In The Post-Pandemic Caribbean, Rajan Bhattarai
Effective Strategies To Attract And Retain Qualified Hospitality Staff In The Post-Pandemic Caribbean, Rajan Bhattarai
Walden Dissertations and Doctoral Studies
Employee attraction and retention remain critical constraints for Caribbean luxury-wellness resorts in the post-pandemic era. Hospitality and tourism leaders are concerned because persistent vacancy, churn, and extended time-to-competence impair service quality and performance of their organizations. Grounded in the Baldrige Excellence Framework and social exchange theory, the purpose of this qualitative single case study was to explore the effective strategies employed by tourism leaders to attract and retain qualified staff within a Caribbean hospitality organization. Data were collected from five Caribbean leaders (HR director, department manager, and supervisors) using semistructured interviews and analyzing organizational documents. Thematic analysis was used to …
Perceived Effects Of Verbal Orders On In-Patient Medication Reconciliation And Safety: A Multi-Center Cross-Sectional Study, Yahaya Jafaru, Abubakar K. Sahabi, Mohammed M. Sani
Perceived Effects Of Verbal Orders On In-Patient Medication Reconciliation And Safety: A Multi-Center Cross-Sectional Study, Yahaya Jafaru, Abubakar K. Sahabi, Mohammed M. Sani
Pharmacology and Clinical Pharmacy Research
Medication reconciliation presents significant challenges for healthcare professionals, particularly due to communication gaps that contribute to patient safety incidents. The aim of the study was to investigate the perceived effect of verbal orders on in-patient medication reconciliation and safety. The study was a cross-sectional survey with nurse employees of the three tertiary hospitals in Kebbi State, Nigeria as population of the study. The instrument for the data collection was a 21-items researcher-constructed self-administered questionnaire. Chi-square and binomial regression analysis were used in data analysis. Over two-thirds (70.7%) of the respondents reported a negative perceived effect on the patient’s compliance. The …
Crosstalk Between Gut Microbiota And Antihypertensive Drugs: A Systematic Review, Dhiya'ul Haq, Keri Lestari
Crosstalk Between Gut Microbiota And Antihypertensive Drugs: A Systematic Review, Dhiya'ul Haq, Keri Lestari
Pharmacology and Clinical Pharmacy Research
Hypertension is a multifactorial condition characterized by persistently elevated blood pressure. In addition to genetic and environmental factors, recent studies suggest that the gut microbiota contributes to the development of hypertension and modulates the response to antihypertensive therapy. This review aims to summarize the latest knowledge on the bidirectional interaction between gut microbiota and antihypertensive drugs. Original studies published up to May 2025 on this topic were collected from two online databases: PubMed and Scopus. A total of 220 articles were identified, and 9 articles met the inclusion criteria for further discussion. Three of these included articles discussed how gut …
Model For Calculating Impact Force For Individualized Hip Fracture Prediction During A Fall, Alisha Agarwal, Daniel Kargilis, Nishtha Gupta, Michael Chang, Rui Feng, Gregory Chang, Chamith S. Rajapakse
Model For Calculating Impact Force For Individualized Hip Fracture Prediction During A Fall, Alisha Agarwal, Daniel Kargilis, Nishtha Gupta, Michael Chang, Rui Feng, Gregory Chang, Chamith S. Rajapakse
Student Papers, Posters & Projects
Osteoporotic-related weakening of bone is a common cause of hip fractures. The standard of care for the diagnosis and management of osteoporosis is the dual-energy x-ray absorptiometry bone mineral density T-scores. Many individuals considered nonosteoporotic, however, still sustain fractures since these tools do not incorporate vital bone parameters and subject-specific characteristics. The purpose of this work was to (1) develop a simple analytical model for estimating the force exerted on the femur during a fall (i.e., impact force) based on measurable patient metrics and (2) define a quantifiable fracture risk index by comparing finite-element-derived bone strength and impact force, which …
Fgf13 Regulates Vgsc-Independent Cardiomyocyte Impulse Propagation Via Cx43 Trafficking, Lala Tanmoy Das, Mattia Malvezzi, Aravind R Gade, Maiko Matsui, Margaret Mckay, Eric Q Wei, Matea J Zelich, Keon Mazdisnian, Jared Kushner, Bi-Xing Chen, Isabella Distefano, Daniel Roybal, Lin Yang, Lisa Stoll, James C Lo, Marian Kalocsay, Fadi G Akar, Steven O Marx, Geoffrey S Pitt
Fgf13 Regulates Vgsc-Independent Cardiomyocyte Impulse Propagation Via Cx43 Trafficking, Lala Tanmoy Das, Mattia Malvezzi, Aravind R Gade, Maiko Matsui, Margaret Mckay, Eric Q Wei, Matea J Zelich, Keon Mazdisnian, Jared Kushner, Bi-Xing Chen, Isabella Distefano, Daniel Roybal, Lin Yang, Lisa Stoll, James C Lo, Marian Kalocsay, Fadi G Akar, Steven O Marx, Geoffrey S Pitt
Faculty, Staff and Student Publications
Background: FHF (fibroblast growth factor homologous factor) variants associate with arrhythmias. Although FHFs are best characterized as regulators of voltage-gated sodium channel (VGSC) gating, recent studies suggest broader, non-VGSC-related functions, including regulation of Cx43 (connexin 43) gap junctions and hemichannels, mechanisms that have generally been understudied or disregarded.
Methods: We assessed cardiac conduction and cardiomyocyte action potentials in mice with constitutive cardiac-specific Fgf13 ablation (cFgf13KO) while targeting Cx43 gap junctions and hemichannels pharmacologically. We characterized FGF13 regulation of Cx43 abundance and subcellular distribution. With proximity labeling proteomics, we investigated novel candidate mechanisms underlying FGF13 regulation of Cx43. …
Periodontal Precision: Diagnostic Skills And Confidence Of Dentists In Asian Countries In Applying The 2017 Efp/Aap Periodontal Disease Classification- A Cross-Sectional Pilot Study, Kamran Ali, Priti Charde, Daniel Zahra, Ewen Mccoll, Tayeb Al Hadeethi, Nader Hamdan
Periodontal Precision: Diagnostic Skills And Confidence Of Dentists In Asian Countries In Applying The 2017 Efp/Aap Periodontal Disease Classification- A Cross-Sectional Pilot Study, Kamran Ali, Priti Charde, Daniel Zahra, Ewen Mccoll, Tayeb Al Hadeethi, Nader Hamdan
Peninsula Dental School
Background: The classification of periodontal disease published in 2017 by the European Federation of Periodontology (EFP) and American Academy of Periodontology (AAP), provides a framework for diagnosis and treatment. The aim of this study was to evaluate the diagnostic skills and self-perceived confidence of dentists and dental students based in Asian countries in the use of this classification. Methods: A cross-sectional analytic study design was employed. An online questionnaire encompassing four periodontitis cases was used for data collection. A total of 500 participants were invited to provide a diagnosis and rate their confidence for each case. Results: Responses were provided …
Heart-Centered Discernment In Leadership: Pope Francis’S Teachings In Dilexit Nos, Jessica Christie Ludescher Imanaka
Heart-Centered Discernment In Leadership: Pope Francis’S Teachings In Dilexit Nos, Jessica Christie Ludescher Imanaka
Journal of Management for Global Sustainability
This paper articulates the central teachings of Pope Francis’s final encyclical, Dilexit Nos, and explains how these teachings can guide business leadership. Dilexit Nos offers spiritual guidance “on the human and divine love of the Heart of Jesus Christ.” This spiritual encyclical concludes with a reminder about the importance of the love of Christ for the work to which the social encyclicals Laudato Si’ and Fratelli Tutti call us. Pope Leo XIV recently clarified the import of Dilexit Nos for our love for the poor in an apostolic exhortation, Dilexi Te, which Francis had been writing prior to his death. …
The Impact Of Artificial Intelligence On Employee Attrition And Organizational Profitability, Saeed Almehairi
The Impact Of Artificial Intelligence On Employee Attrition And Organizational Profitability, Saeed Almehairi
Theses
Artificial Intelligence (AI) has become a transformative force in the modern workplace, reshap- ing decision-making processes, enhancing operational efficiency, and influencing the dynamics of human resource management. One of the critical areas where AI demonstrates substantial potential is in predicting, managing, and mitigating employee attrition. Employee attrition remains a pressing organizational challenge, incurring substantial costs in recruitment, training, and lost productivity. This research investigates the dual impact of AI on employee attrition and organizational profitability, exploring both the opportunities and the challenges that arise from its integration into human resource and business operations. The study examines how AI-driven predictive analytics …
Increased Intermembrane Space [Ca2+] Drives Mitochondrial Structural Damage In Cpvt, Shanna Hamilton, Radmila Terentyeva, Roland Veress, Fruzsina Perger, Zuzana Nichtova, Mark Bannister, Jinxi Wang, Sage Quiggle, Rachel Battershell, Matthew Gorr, Sandor Györke, Bum-Rak Choi, Christopher George, Andriy Belevych, György Csordás, Dmitry Terentyev
Increased Intermembrane Space [Ca2+] Drives Mitochondrial Structural Damage In Cpvt, Shanna Hamilton, Radmila Terentyeva, Roland Veress, Fruzsina Perger, Zuzana Nichtova, Mark Bannister, Jinxi Wang, Sage Quiggle, Rachel Battershell, Matthew Gorr, Sandor Györke, Bum-Rak Choi, Christopher George, Andriy Belevych, György Csordás, Dmitry Terentyev
Department of Pathology, Anatomy, and Cell Biology Faculty Papers
BACKGROUND: Mitochondrial dysfunction caused by abnormally high RyR2 (ryanodine receptor) activity is a common finding in cardiovascular diseases. Mechanisms linking RyR2 gain of function with mitochondrial remodeling remain elusive. We hypothesized that RyR2 hyperactivity in cardiac disease increases [Ca 2+ ] in the mitochondrial intermembrane space (IMS) and activates the Ca 2+ -sensitive protease calpain, driving remodeling of mitochondrial cristae architecture through cleavage of structural protein OPA1 (optic atrophy protein 1).
METHODS: We generated a highly arrhythmogenic rat model of catecholaminergic polymorphic ventricular tachycardia, induced by RyR2 gain-of-function mutation S2236L(Ser2336Leu)(+/-) . We created a new biosensor to measure IMS-[Ca2+ ] …