Digital Commons Network^™

A Better Way To Make Protein, James Gillette Bosco

Graduate Student Theses, Dissertations, & Professional Papers

Producing recombinant proteins stands as a cornerstone in both biotechnology and pharmaceutical research, supporting the creation of therapeutics, vaccines, and enzymes used in various industries. Bacterial systems for expression, especially Escherichia coli, are preferred due to their rapid growth rates, ease of genetic manipulation, and scalability. However, generating proteins under current Good Manufacturing Practice (cGMP) standards introduces substantial challenges, such as issues with scalability, toxicity, protein misfolding, and strict regulatory demands. This section delivers an in-depth review of these obstacles and current methods developed to address them. An extensive exploration is conducted into induction techniques, including auto-induction and isopropyl-D-1-thiogalactopyranoside (IPTG) …

Agitprop: A Deep Dive Into Leftist Affinity Spaces, Brittany M. Fuher

Cal Poly Humboldt theses and projects

This project is designed to explore what methods and theories of how we learn can tell us about what is occurring in leftist digital affinity spaces. My focus is the HasanAbi community, an online collective built around Twitch streamer Hasan Piker. I will examine the way YouTubers create memetic systems using an understanding of semiotics and translingualism. Based on my own experiences with digital community, and inspired by Linda Adler-Kassner, and Elizabeth Wardle’s discussion of Threshold Concepts in Naming What We Know, I’ve created five of my own Threshold Concepts for Leftist Community and crafted a video for each. …

Assessing Sustainability: Knowledge, Behaviors, And Attitudes Before And After College, Seth Blair Comella Bradley

Cal Poly Humboldt theses and projects

Cal Poly Humboldt has been assessing the sustainability literacy and culture among its new and graduating students for over five years using a custom survey with a longitudinal design. Using data from Fall 2018 to Spring 2023, this project is both an exploration into changes in student sustainability attitudes, behaviors, and knowledge from when they start as new students to when they graduate as well as an in-progress assessment of the survey. Results indicate the majority of undergraduates value sustainability, although behaviors and knowledge did not always align with those beliefs. While no statistically significant changes were detected in longitudinal …

Two-Dimensional Hydrodynamic Model Comparison For Fish Habitat Assessment In Rivers, Tyler Caseltine

Cal Poly Humboldt theses and projects

Accurate prediction of aquatic habitat availability for salmonids and other aquatic species is crucial for effective river and tributary restoration and management. Two-dimensional (2D) hydrodynamic models have become the standard for simulating depth and velocity conditions in open channel systems, leveraging advances in computational capabilities and spatial data collection. This study compares four 2D hydrodynamic models—SToRM, River2D, HECRAS2D, and SRH2D—using consistent topographic and calibration data, and adhering to Quality Assurance and Quality Control (QA/QC) standards as outlined by the US Fish and Wildlife Service (USFWS) and McBain Associates. The models were evaluated with data from two study sites for their …

Measuring Real-Time Medication Effects From Electroencephalography, William Bosl, Aarti Sathyanarayana, Rima El Atrache, Michele Jackson, Sarah Cantley, Latania Reece, Claire Ufongene, Tobias Loddenkemper, Kenneth Mandl

Nursing and Health Professions Faculty Research and Publications

No abstract provided.

N Y State Dent J January 2024

The New York State Dental Journal

In the January 2024 issue, the reader will find the following feature articles:

• When One Door Closes, Another Opens
• Oral Manifestations of Metastatic Diseases: A Clinical and Histologic Analysis of Seven Cases
• Dentistry Transformed: An Overview of Artificial Intelligence’s Role in Dental Care
• Unlocking the Future of Dentistry: A Summary of AI and Augmented Intelligence in Dentistry from ADA’s White Paper
• Diagnostic Detail: Understanding the Impact of Tooth Rotation in Correcting Mucogingival Defects

This issue includes regular columns with regional news impacting the New York membership including: editorial and perspectives columns, legal, association activities, component news, continuing education opportunities, and …

Complexation Of Diserinol Isophthalamide With Phosphorylated Biomolecules In Electrospray Ionization Mass Spectrometry, Madeline Schultz, Neil A. Ellis, Nwanne D. Banor, Daniel A. Thomas

Chemistry Faculty Publications

Electrospray ionization (ESI) enables gentle transfer of biomolecules from solution to vacuum, facilitating the study of biomolecular structure under highly controlled conditions. However, biomolecules are desolvated during the ESI process, and the loss of ionic hydrogen bonds to solvent molecules can drive structural rearrangement, most prominently at solvent-exposed charge sites. Microsolvation reagents can bind to these bare charge sites in ESI mass spectrometry (ESI–MS) experiments, providing alternative intermolecular interaction partners. Previously, 18-crown-6 was shown to be an effective reagent for binding to cationic monoalkylammonium residues. More recently, diserinol isophthalamide (DIP) was reported as an analogous anionic microsolvation reagent, primarily for …

Acceptability Of Anal Human Papillomavirus Home Self-Sampling And Clinician Sampling Among Sexual And Gender Minority Individuals In Milwaukee, Wisconsin: The Prevent Anal Cancer Self-Swab Study, Jenna Nitkowski, Anna R Giuliano, Tim Ridolfi, Elizabeth Chiao, Maria E Fernandez, Vanessa Schick, Michael D Swartz, Jennifer S Smith, Alan G Nyitray

Faculty, Staff and Student Publications

Purpose: Anal cancer has disproportionately high incidence among sexual minority men. We compared acceptability of home versus clinic human papillomavirus (HPV) anal swabbing.

Methods: The Prevent Anal Cancer Self-Swab Study recruited sexual and gender minority individuals in Milwaukee, Wisconsin. Eligible participants were randomized to a home or clinic arm. Home participants received a mailed anal HPV self-sampling kit. Clinic participants attended a clinic appointment where a clinician collected an anal HPV swab. We examined acceptability (overall thoughts, comfort with method, pain, and future willingness to swab) of home versus clinic swabbing using postswab survey responses.

Results: A total of 191 …

Human Papillomavirus (Hpv) Vaccination In Males: Associations Of Hpv-Related Knowledge And Perceptions With Hpv Vaccination Intention Among Korean Mothers Of Boys, Jihye Choi, Paula Cuccaro, Christine Markham, Sooyoun Kim, Irene Tamí-Maury

Faculty, Staff and Student Publications

OBJECTIVES: Human papillomavirus (HPV) vaccination rates among males are suboptimal worldwide. In South Korea, little is known about parents' intention to vaccinate boys against HPV. Therefore, we examined the associations of HPV-related knowledge and perceptions with vaccination intention among Korean mothers of boys.

METHODS: From August to December 2021, eligible mothers were surveyed about HPV-related knowledge, perceptions, and intention to vaccinate their sons against HPV. Mothers were categorized into no intention, contemplating, and intention to vaccinate groups. Using analysis of variance, we compared the groups' scores on the scales measuring HPV-related knowledge, attitudes, and beliefs. We conducted a multinomial logistic …

A Randomized Controlled Study Of Auricular Point Acupressure To Manage Chemotherapy-Induced Neuropathy: Study Protocol, Nada Lukkahatai, Michael V Nguyen, Jingyu Zhang, Yu-Min Cho, Chitchanok Benjasirisan, Heijingzi Monica Jia, Claudia M Campbell, Jennifer Kawi, Hulin Wu, Hongyu Wang, Rupsikha Bora, Johannes Thrul, Constance M Johnson, Thomas J Smith

Faculty, Staff and Student Publications

Objective: Chemotherapy-induced neuropathy (CIN) significantly impacts cancer patients, leading to functional disability, diminished quality of life, and increased healthcare costs amid the ongoing opioid crisis. Auricular point acupressure (APA), a non-invasive and non-pharmacological alternative, has shown potential for alleviating the pain, numbness, and tingling associated with CIN. This study aims to assess the efficacy of APA for CIN symptoms and physical function and to examine the mechanisms underlying APA's effects on CIN.

Methods: This is a three-arm randomized controlled clinical trial protocol. Patients aged 18 and older who are experiencing CIN are randomly assigned to one of the three groups: …

Beside Marriage Equality: Conversations On Supriyo, Danish Sheikh, Rupali Samuel

Socio-Legal Review

This case comment takes the form of an epistolary exchange between the authors as they reflect on the Indian Supreme Court’s 2023 judgment in Supriyo @ Supriya Chakraborty & Anr v Union of India, which denied the existence of a constitutional right to marriage and deferred the responsibility of legalising same-sex marriage to the legislature. Through a staged conversation, the comment explores personal subject positions, legal frameworks, and the intricate entanglements of intimacy and state recognition. The authors consider how the law leaves imprints on our bodies and, in turn, how our embodied lives reshape the law through acts …

Cultivating Attentiveness To Law In India Through Legal Anthropology, Deepa Das Acevedo, Jahnavi Chamarthi

Socio-Legal Review

This Article reviews and analyses scholarship at the intersection of anthropology and law. The first half of the Article provides context for understanding the boundaries, animating concerns, and tensions that have characterised the anthropology of law as an area of interdisciplinary inquiry. We focus especially on the subdiscipline’s Anglo-American history and show how a promising early period of engagement dissipated as both anthropologists and legal scholars lost enthusiasm for each others’ insights and methods. Then, we expand our analysis outward. Because legal anthropology belongs within, and is increasingly attentive to, the broader field of law and society scholarship, we explore …

A Single-Centre Registry In Impella-Supported High-Risk Percutaneous Coronary Intervention — Our 5 Year Experience, Karen Pui Shan Shek, Jonathan Gabriel Sung, Ling Ling Ip, Ho Lam, Ping Wa Yam

Journal of the Hong Kong College of Cardiology

Background

Complex, high-risk and indicated PCI with impaired left ventricular function remains a challenge. The Impella device offers hemodynamic support during HR-PCI to maintain organ perfusion. There are trials and registries on experience with Caucasians. However, Asian data have been limited.

Methods

In this single-centre, retrospective and observational registry, 38 patients underwent elective or urgent HR-PCI with the Impella® CP between June 2018 and June 2023 in Tuen Mun Hospital. Primary feasibility end points were successful device implantation, freedom from unstable ventricular arrhythmia and cardiac arrest. Primary safety end points were 30-day mortality and MACCE. Secondary safety end points were …

Neurodevelopmental Vulnerability To Gestational Ozone Exposure, Vishnupriya Alavala, Sarah E. Brent, Christopher G. Canal, Joseph Wang, Russell P. Hunter, Matthew J. Campen, Andrew K. Ottens

UROP Posters

Ambient air pollution accounts for about 4.2 million premature deaths annually per the World Health Organization. Ozone (O3) is a highly reactive air pollutant found in smog and is implicated in cellular damage leading to organ dysfunction. Ambient air pollution is associated with neurodevelopmental, behavioral, and cognitive disorders though ozone’s role is unknown. Studies here look at ozone exposure shortly after implantation vs. shortly before term to evaluate differences in neurodevelopmental susceptibility over time.

To inquire on the effects of ozone on the fetal brain, pregnant Sprague-Dawley rats were exposed once to 0.3 ppm of O3 or filtered air (FA) …

"Who The Hell Am I? What Am I Bringing In Here?": A Phenomenographic Exploration Of Antiracist Mentor Development, Kelly Lynn Mack

Dissertations

Teacher attitudes toward racial diversity profoundly impact students, especially when compounded by teacher and student demographic disparities. Despite the purported goal of addressing biases and deficit thinking that limit teachers' ability to support all learners, antiracist pedagogy in teacher credentialing is poorly understood. Also included in the credentialing process, newly qualified teachers (NQTs) in California engage in a two-year induction program, collaborating closely with an induction mentor to attain their clear credential. Employing a qualitative, phenomenographic approach, this study explored how mentors within a longstanding induction program understood and implemented antiracist pedagogy. The research uncovered significant insights into (a) dimensions …

Heart Rate Variability Is Reduced In Covid-19 Survivors And Associated With Physical Activity And Fatigue, Michael H. Haischer, Lauren E. Opielinski, Lindsey M. Mirkes, Toni D. Uhrich, Rachel E. Bollaert, Michael E. Danduran, Marie K. Hoeger Bement, Linda B. Piacentine, Paula E. Papanek, Sandra K. Hunter

Exercise Science Faculty Research and Publications

Reduced heart rate variability (HRV) and fatigue are common after COVID-19 infection and both are potentially influenced by physical activity (PA). We compared resting HRV, PA from accelerometers and questionnaires, and self-reported fatigue in 41 COVID-19 survivors (~8 months postinfection, 38 ± 17 years) with 41 matched controls. Differences in HRV were observed on acceleration capacity (p = 0.041), deceleration capacity (p = 0.032), high-frequency peak frequency (p = 0.019), absolute low-frequency power (p = 0.042), relative very low-frequency power (p = 0.012), SD2 (from Poincare plot; p = 0.047), and DFA2 (slope of long-term …

Middle-Range Scores From The Patient Determined Disease Steps Scale Reflect Varying Levels Of Walking Dysfunction In Multiple Sclerosis, Robert W. Motl, Whitney Neal, Deborah Backus, Jeffrey Hebert, Kevin K. Mccully, Francois Bethoux, Prudence Plummer, Alexander V. Ng, John Lowman, Hollie Schmidt, Robert Mcburney, Gary R. Cutter

Exercise Science Faculty Research and Publications

Background

Multiple sclerosis (MS) is a leading cause of neurological disability among young and middle-aged adults worldwide, and disability is measured using a variety of approaches, including patient reported outcome measures (PROMs) such as the Patient Determined Disease Steps (PDDS) scale. There is limited evidence for the validity of inferences from the middle-range of scores on the PDDS (i.e., 3 “gait disability” – 6 “bilateral support”), but that range of scores seemingly represents moderate disability characterized by varying levels of walking dysfunction.

Purpose

The current study examined whether the middle-range of scores from the PDDS reflect varying levels of walking …

Determinants Of Sars-Cov-2 Igg Response And Decay In Canadian Healthcare Workers: A Prospective Cohort Study, Nicola Cherry, Anil Adisesh, Igor Burstyn, Carmen Charlton, Yan Chen, Quentin Durand-Moreau, France Labrèche, Shannon Ruzycki, Leeann Turnbull, Tanis Zadunayski, Yutaka Yasui

Études primaires

Introduction: Healthcare workers (HCWs) from an interprovincial Canadian cohort gave serial blood samples to identify factors associated with anti-receptor binding domain (anti-RBD) IgG response to the SARS-CoV-2 virus. Methods: Members of the HCW cohort donated blood samples four months after their first SARS-CoV-2 immunization and again at 7, 10 and 13 months. Date and type of immunizations and dates of SARS-CoV-2 infection were collected at each of four contacts, together with information on immunologically-compromising conditions and current therapies. Blood samples were analyzed centrally for anti-RBD IgG and anti-nucleocapsid IgG (Abbott Architect, Abbott Diagnostics). Records of immunization and SARS-CoV-2 testing from …

A Quantitative Method To Guide The Integration Of Textile Inductive Electrodes In Automotive Applications For Respiratory Monitoring, James Elber Duverger, Victor Bellemin, Patricia Forcier, Justine Decaens, Ghyslain Gagnon, Alireza Saidi

Études primaires

Induction-based breathing sensors in automobiles enable unobtrusive respiratory rate monitoring as an indicator of a driver’s alertness and health. This paper introduces a quantitative method based on signal quality to guide the integration of textile inductive electrodes in automotive applications. A case study with a simplified setup illustrated the ability of the method to successfully provide basic design rules about where and how to integrate the electrodes on seat belts and seat backs to gather good quality respiratory signals in an automobile. The best signals came from the subject’s waist, then from the chest, then from the upper back, and …

Robust Testing For Explosive Behavior With Strongly Dependent Errors, Yui Lim Lui, Peter C. B. Phillips, Jun Yu

Research Collection School Of Economics

A heteroskedasticity-autocorrelation robust (HAR) test statistic is proposed to test for the presence of explosive roots in financial or real asset prices when the equation errors are strongly dependent. Limit theory for the test statistic is developed and extended to heteroskedastic models. The new test has stable size properties unlike conventional test statistics that typically lead to size distortion and inconsistency in the presence of strongly dependent equation errors. The new procedure can be used to consistently time-stamp the origination and termination of an explosive episode under similar conditions of long memory errors. Simulations are conducted to assess the finite …

High-Dimensional Iv Cointegration Estimation And Inference, Peter C. B. Phillips, Igor L. Kheifets

Research Collection School Of Economics

A semiparametric triangular systems approach shows how multicointegrating linkages occur naturally in an I(1) cointegrated regression model when the long run error variance matrix in the system is singular. Under such singularity, cointegrated I(1) systems embody a multicointegrated structure that makes them useful in many empirical settings. Earlier work shows that such systems may be analyzed and estimated without appealing to the associated I(2) system but with suboptimal convergence rates and potential asymptotic bias. The present paper develops a robust approach to estimation and inference of such systems using high dimensional IV methods that have appealing asymptotic properties like those …

Board 438: Year Two Of Developing A New Dataset For Analyzing Engineering Curricula, David Reeping, Kenneth Reid, Matthew Ohland, Nahal Rashedi

School of Engineering Education Faculty Publications

This paper discusses the developments during Year 2 for a project concerned with analyzing the curricula of engineering programs in the United States to understand the structural barriers embedded in degree requirements that could push out diverse groups of students. We are using an emerging method for quantifying the complexity of these programs called Curricular Analytics. This method involves treating the prerequisite relationships between courses as a network and applying graph theoretic measures to calculate a curriculum’s structure complexity. In Year 1, we collected 497 plans of study representing five engineering disciplines (i.e., Mechanical, Civil, Electrical, Chemical, and Industrial) across …

Digitizing Historical Resources On The Ohio & Erie Canal, S. Victor Fleischer

University Research

Louis Baus, photographer, shot numerous scenes along the Ohio & Erie, Miami & Erie, and the connecting feeder canals. This collection of over 400 photo negatives portrays a sense of canal life during the late 1800s to early 1900s. Found in this collection are images of canal boats, crew and passengers, as well as various locks, businesses and homes along the canal ways.

The Challenges And Rewards Of Carrying Out Qualitative Research On The Police In The African American Community, Daniel K. Pryce, Ingrid Phillips Witaker

Sociology & Criminal Justice Faculty Publications

In this paper, we discuss the challenges and rewards of carrying out qualitative research on the police in the African American1 community. Using data drawn from interviews with seventy-seven African American adults in Durham, NC, we found that community member hostility toward research(ers) and fear of both neighbors and the police lowered African Americans’ willingness to be interviewed about their perceptions of and experiences with U.S. police. These findings were observed primarily in public housing and middle-income communities. On a positive note, we found that greater awareness of policing issues increased African Americans’ willingness to participate in research about the …

The Freshman Life And Student Health (Flash) Study: Using Social Network Analysis To Understand How Obesity And Stigma Impact Emerging Adult Health, Alena C. Borgatti

All ETDs from UAB

Both racial discrimination and weight stigma have been identified as risk factors for poorer mental, physical, and social health. College students are vulnerable to the effects of stigma, as they greatly value peer acceptance and friendships during a pivotal time of emotional and health behavior development. The present study was the first to use social network analysis to examine the impact of weight and racial discrimination on the development of college social relationships and how these experiences influence students’ physical and mental health. 1330 participants (69% of on-campus freshmen) completed a brief survey on social networks, physical health, mental health, …

The Role Of Fibroblast Growth Factor Receptors In Inflammatory Airway Diseases, Molly Easter

All ETDs from UAB

Chronic inflammation plays a role in the development and progression of airway diseases such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF). COPD is caused primarily by chronic cigarette smoke exposure. Conversely, CF is a single gene disorder caused by mutations in the cystic fibrosis transmembrane conductance regulator gene (CFTR). Although the causes of COPD and CF are different, there are common pathological features. Studies show that chronic inflammation plays a significant role in the pathogenesis of both COPD and CF. Moreover, chronic inflammation induces ageing-associated pathomechanisms like cellular senescence, which exacerbates chronic inflammation. Previous work demonstrates that …

Non-Catalytic Regulators Of The Secretory Pathway, Peyton Vanwinkle

All ETDs from UAB

This thesis focuses on two key questions: 1) how are bone development and regeneration regulated by the CREB3L1 transcription factor; and 2) what is the mechanism of action of the endoplasmic reticulum resident protein JAGN1, which when dysregulated, causes severe congenital neutropenia (SCN)? CREB3L1 regulates bone development and regeneration in mammalian models by facilitating the expression of COL1A1 and components of the secretory pathway required for collagen secretion. We generated two zebrafish models of Creb3l1 dysfunction (creb3l1ΔbZIP/ΔbZIP and creb3l1TA+/TA+). Both models showed that Creb3l1 functions in regulating col1a1a transcription and overall regenerate size are conserved in zebrafish. However, we also …

Utilization Of Romosozumab During Covid-19 Pandemic Among U.S. Medicare Beneficiaries, Ye Liu

All ETDs from UAB

Osteoporosis is increasingly recognized as a major public health issue in the aging U.S. population. Shortly after the licensure of romosozumab, an injectable medication indi-cated for women with postmenopausal osteoporosis at high risk of fracture administered by health care provider monthly, the COVID-19 pandemic broke out, which caused ma-jor disruptions to the delivery of health care across the nation. The pandemic dispropor-tionately affected minority and economically disadvantaged communities, exacerbating many pre-existing health disparities. Romosozumab has the highest dosing frequency among common injectable osteoporosis medications in the U.S. that require an office visit for injection, making romosozumab adherence more susceptible to …

Diagnostic Imaging Approaches: Pet Imaging In Monitoring Immune Responses In Oncology And Tuberculosis, Chanelle Hunter

All ETDs from UAB

Positron emission tomography (PET) imaging agents play a transformative role in personalized medicine through their application in disease diagnosis and immunotherapy monitoring, focusing on chemokine receptors, TH1-activated immune cells, and tuberculosis (TB) diagnostics. In immuno-oncology, accurately assessing TH1-activated immune cells in the tumor microenvironment (TME) is vital for evaluating cancer immunotherapy efficacy. Chemokine receptors, crucial in cellular signaling and inflammatory reactions, play a significant role in TH1 responses, thus serving as a potential area of interest in elucidating these anti-tumor responses. Through our work a novel CXCR3-targeting immunoPET probe, [89Zr]DFO-αCXCR3, was developed to detect and quantify these cells. This probe …

Education Attainment And Health: Outside The Gender Binary, Brianna Patterson

All ETDs from UAB

Transgender and gender nonbinary individuals, hereafter referred to as trans, report worse health outcomes than trans individuals whose gender identity matches their sex assigned at birth, hereafter referred to as cisgender (Bauer, Hammond, Travers et al. 2009, Cicero, Reisner, Merwin et al. 2020). Past research has indicated that social determinants of health, such as education attainment, may be responsible for these inequities (Low 2006, The Lancet Public 2020). Education’s influence on health is well known from previous research, but the association is not distributed equally. In the United States (US) educational system, state and local governments have control, not the …