Digital Commons Network^™

Characterization Of Two Krab-Containing Zinc Finger Transcription Factors In Bovine Preimplantation Embryonic Development, Mingxiang Zhang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oocyte developmental competence or oocyte intrinsic quality describes the capability of oocytes to resume meiosis, cleave and develop to blastocyst stage after fertilization, implant and develop to term in a good health. A growing number of evidences indicate that the majority of embryonic mortality occurs during early embryonic development in different species, including human, horse and cattle primarily due to poor oocyte quality. Maternal effect genes are key aspects of oocyte quality, which are transcribed during the process of oogenesis and folliculogenesis. The maternal factors are accumulated in oocytes, orchestrating various early developmental events including fertilization, epigenetic reprogramming and zygotic …

Application Of Artificial Intelligence For Co2 Storage In Saline Aquifer (Smart Proxy For Snap-Shot In Time), Marwan Mohammed Alnuaimi

Graduate Theses, Dissertations, and Problem Reports (ETD)

In recent years, artificial intelligence (AI) and machine learning (ML) technology have grown in popularity. Smart Proxy Models (SPM) are AI/ML based data-driven models which have proven to be quite crucial in petroleum engineering domain with abundant data, or operations in which large surface/ subsurface volume of data is generated. Climate change mitigation is one application of such technology to simulate and monitor CO2 injection into underground formations.

The goal of the SPM developed in this study is to replicate the results (in terms of pressure and saturation outputs) of the numerical reservoir simulation model (CMG) for CO2 injection into …

Characterization And Coherent Spin Selective Manipulation Of Quantum Dot Energy Levels, Tristan Anthony Wilkinson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Semiconductor quantum dots (QDs) are promising candidates to fulfill a wide range of applications in real-world quantum computing, communication, and networks. Their excellent optical properties such as high brightness, single-photon purity, and narrow linewidths show potential utility in many areas. In order to realize long term goals of integration into complex and scalable quantum information systems, many current challenges must be overcome. One of these challenges is accomplishment of all necessary computing operations within a QD, which might be enabled by coherent manipulation of single QD energy level structures. In the realm of scalability for quantum devices, a way to …

Understanding Perceptions Of Vulnerable Roadway Users On Autonomous Vehicles, Md Tawhidur Rahman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Public perceptions have been playing an important role in the development of autonomous vehicle (AV) technology. Besides AV and non-AV users, the perceptions of vulnerable roadway users are critical, as AVs will become a part of multimodal transportation system. Pedestrians and bicyclists are among the vulnerable groups of roadway users, as they are relatively unprotected compared to the occupants of AVs or non-AVs. Although AV’s capability to monitor other vehicles has been documented in many studies, there are concerns about AV’s capability in monitoring pedestrians and bicyclists. The overarching goal of this dissertation is to investigate the perceptions of pedestrians …

Appalachian Broadcast News Coverage Of The Coronavirus: A Content Analysis Of Media Framing In The Midst Of A Health Crisis, Jensen M. Mills

Graduate Theses, Dissertations, and Problem Reports (ETD)

Media framing of broadcast news is more than identifying an issue, but rather interpreting and explaining the issue for others to better understand. Previous framing scholarship in broadcast news, as well as COVID-19 specifically, has focused mostly on national or international news, so this research explores broadcast coverage from a local perspective in a rural location. During the health crisis of COVID-19, the specific use of media frames can impact how people made sense of the pandemic. Through a content analysis of 165 newscast scripts from five different local news stations in Appalachia, this study contributes to the understanding of …

The Bigger Picture: Wrongful Conviction Documentary Thematic Vs Episodic Framing, Alecsandra N. Vac

Graduate Theses, Dissertations, and Problem Reports (ETD)

True crime documentaries have gained popularity in the past decade, but those focusing on wrongful conviction cases have been on the rise (Doughty, 2018). Advocates against wrongful conviction, such as the Innocence Project, have been utilizing entertainment media to increase awareness on the issues present in the criminal justice system. However, the framing of wrongful conviction causes and cases (ex: focusing on extreme accusations of police misconduct and intentional forensic evidence tampering) could lead to a bias in how the viewers understand how miscarriages of justice occur. The primary research question being investigated here is, how are these advocacy-recommended documentaries …

Capillary Electrophoresis: Method Development And Application In Biomolecule Analysis, Lindsay Marie Veltri

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Capillary Electrophoresis: Method Development and Application in Biomolecule Analysis

Lindsay Veltri

Biomolecule analysis is essential in disease diagnostics and the development of therapeutic agents. There is a vast array of methods available for biomolecule analysis, however, capillary electrophoresis is well-suited due to minimal sample consumption (~nanoliters of solution) and fast analysis times. In this report, capillary electrophoresis was used to analyze glycosylation of therapeutic proteins. Additionally, a capillary electrophoresis-mass spectrometry method is reported for the analysis of proteins.

Post-translation protein modifications like glycosylation, play an important in protein structure and function. Modifications of protein glycosylation can lead to improved …

The Heart Of Everything In The Middle Of Nowhere: The Role Of Rural Identity In The Formation And Deployment Of Political Attitudes In Pennsylvania, Mikaela G. Zimmerman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The world of American politics continues to infiltrate households across the United States as technological advancement extends the reach of breaking news and government action. With this expanding reach, communities all over the country are digesting and contemplating their place in national politics more fervently than ever. At the crux of this discussion is the backbone of political engagement and action—identity and its resulting political attitudes. For decades, partisanship has been a point of contention amongst American citizens. Cities across the nation showcase protests, demonstrations, town hall meetings, and more illustrating citizens’ care for their democratized input in government affairs. …

Medicaid In The Orthodontic Community: Provider Trends And Perceptions, Miranda L. Fabrega

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives: Medicaid is a federal and state insurance program aimed at helping economically disadvantaged individuals and families with gaining access to health care. Lack of provider participation in the Medicaid program leaves many Medicaid-eligible individuals with the inability to obtain certain medical services, such as orthodontic care. The objective of this study was to use an electronic survey to better understand the beliefs and behaviors of orthodontists in regard to participation in the Medicaid program, and to determine if differences exist between Medicaid providers and non-Medicaid providers. Experimental Design and Methods: Survey: Requests to participate in an electronic …

Critical Perspectives On Produce Prescription Programs & Us Federal Nutrition Policy, Alanna K. Higgins

Graduate Theses, Dissertations, and Problem Reports (ETD)

Produce prescription programs, interventions operating at the intersection of food access and public health, are steadily increasing in number across the United States since 2010. I leverage key informant interviews, participant observations, and event ethnographies to form a four-year institutional ethnography of the implementation of produce prescriptions within West Virginia alongside a legal-policy archaeology methodology to understand how produce prescriptions have been institutionalized and funded within the US Farm Bill. While much of produce prescription program growth is attributed to an expansion of federal funding starting in 2014, this dissertation demonstrates that these programs and the federal legislation which has …

The Lived Experiences Of Selected Choral Directors During The Covid-19 Pandemic, Angela Berna Milliren

Graduate Theses, Dissertations, and Problem Reports (ETD)

The shift to online education during the COVID-19 pandemic found secondary choral teachers moving traditional performance-based courses to the online venue. The pedagogical changes needed include implementing technology and disseminating information through learning management systems. Relationships between teachers and students, and teachers and colleagues, were challenged with the physical distance of quarantines in the Spring of 2020. This dissertation research project examined the difficulties six teachers faced concerning technology and relationships. I sat down for semi-structured interviews with six colleagues where I asked about their backgrounds, relationships with students and fellow colleagues, and the changes the pandemic brought to their …

Zonulin As A Mediator Of Psychological Stress And Periodontal Disease, Casey D. Wright

Graduate Theses, Dissertations, and Problem Reports (ETD)

The prevalence of chronic inflammatory diseases is on the rise. Some have posited the permeability of tight junctions in gastrointestinal epithelium tissues as a potential mechanism for precipitating inflammatory processes throughout the body. Zonulin is the only known modulator of tight junction permeability and has been implicated in numerous chronic inflammatory processes (e.g., proinflammatory cytokine production) and diseases, more generally. The role of zonulin in oral inflammation, however, has yet to be explored. Periodontal disease is the most common oral inflammatory condition and primary perpetrator of tooth loss. Periodontal disease also is associated with a number of other health problems …

Modeling, Fabrication, And Characterization Of Rf-Based Passive Wireless Sensors Composed Of Refractory Semiconducting Ceramics For High Temperature Applications, Kavin Sivaneri Varadharajan Idhaiam

Graduate Theses, Dissertations, and Problem Reports (ETD)

Real-time health monitoring of high temperature systems (>500^oC) in harsh environments is necessary to prevent catastrophic events caused by structural failures, varying pressure, and chemical reactions. Conventional solid-state temperature sensors such as resistance temperature detectors (RTDs) and thermocouples are restricted by their operating environments, sensor dimensions and often require external power sources for their operation. The current work presents the research and development of RF-based passive wireless sensing technology targeting high temperatures and harsh environmental conditions. Passive wireless devices are generally classified as near-field and far-field devices based on the interrogation distance. Near-field sensors are placed at …

Computational Analysis Of The Stability Limits Of Premixed Methane-Air Combustion In Micro-Channels, Almoutazbellah Adnan Kutkut

Graduate Theses, Dissertations, and Problem Reports (ETD)

Technological progress in the last decades facilitated the development of integrated microsystem devices, including electrical and mechanical components for micro-electro-mechanical systems (MEMS). Such systems necessitate an external power supply, but typical batteries yet have some unavoidable limitations. To address this problem, numerous studies have been conducted to develop alternatives powered by micro combustion – in order to effectively utilize the high-density energy of hydrocarbon fuels. Such a global need has resulted in much more attention devoted to the combustion-based micro-power generation systems. However, to better understand the typical combustion characteristics in such microscale systems, design and performance restrictions need to …

Impact Of Radio Frequency Interference And Real-Time Spectral Kurtosis Mitigation, Evan T. Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

We catalog the ubiquity of Radio Frequency Interference (RFI) plaguing every modern radio telescope and investigate several ways to mitigate it in order to create better science-ready data products for astronomers. There are a myriad of possible RFI sources, including satellite uplinks and downlinks, cellular communications, air traffic radar, and natural sources such as lightning. Real-time RFI mitigation strategies must take these RFI characteristics into account, as the interfering signals can look significantly different at very high time and frequency resolutions.

We examine Spectral Kurtosis (SK) as a real-time statistical RFI detection method, and compare its flagging efficacy against simulated …

Student Understanding Of The Definite Integral When Solving Calculus Volume Problems, Krista Bresock

Graduate Theses, Dissertations, and Problem Reports (ETD)

The concept of integration appears in many different scientific fields, and students’ understanding of and ability to use the definite integral in applications is important to success in their STEM (science, technology, engineering, and mathematics) classes. One of the first types of application problems that students encounter is finding the volume of a solid using the definite integral. How students approach these problems and how they use the definite integral to find volumes can have an impact on their future use and understanding of the definite integral.

This study involves a deep and thorough investigation of how ten students understand …

Bottleneck Management Through Strategic Sequencing In Smart Manufacturing Systems, Sayantee Roy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nowadays, industries put a significant emphasis on finding the optimum order for carrying out jobs in sequence. This is a crucial element in determining net productivity. Depending on the demand criterion, all production systems, including flexible manufacturing systems, follow a predefined sequence of job-based machine operations. The complexity of the problem increases with increasing machines and jobs to sequence, demanding the use of an appropriate sequencing technique. The major contribution of this work is to modify an existing algorithm with a very unusual machine setup and find the optimal sequence which will really minimize the makespan. This custom machine setup …

System Development Of An Unmanned Ground Vehicle And Implementation Of An Autonomous Navigation Module In A Mine Environment, Jonas Amoama Bredu Jnr

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are numerous benefits to the insights gained from the exploration and exploitation of underground mines. There are also great risks and challenges involved, such as accidents that have claimed many lives. To avoid these accidents, inspections of the large mines were carried out by the miners, which is not always economically feasible and puts the safety of the inspectors at risk. Despite the progress in the development of robotic systems, autonomous navigation, localization and mapping algorithms, these environments remain particularly demanding for these systems. The successful implementation of the autonomous unmanned system will allow mine workers to autonomously determine …

Noise Exposure Trends Among Violating Coal Mines, 2000 To 2021, Hanna Grace Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Noise-induced hearing loss has continued to be a significant issue within the mining industry, even after the passing of the MSHA’s improved noise standard in 2000. NIOSH has recognized that the noise standard enacted by both MSHA and OSHA does not properly protect workers with the 5 dBA exchange rate (i.e., the increase in noise level leading to a doubling of energy) and the lack of emphasizing the use of engineering controls over administrative controls and overreliance on hearing protection device usage. The primary objective of this study is to investigate statistical sound relationships between MSHA noise-related violations and samples …

Artificial Intelligence Based Approach For Rapid Material Discovery: From Chemical Synthesis To Quantum Materials, Robert Tempke

Graduate Theses, Dissertations, and Problem Reports (ETD)

With the advent of machine learning (ML) in the field of Materials Science, it has become obvious that trained models are limited by the amount and quality of the data used for training. Where researchers do not have access to the breadth and depth of labeled data that fields like image processing and natural language processing enjoy. In the specific application of materials discovery, there is the issue of continuity in atomistic datasets. Often if one relies on experimental data mined from literature and patents this data is only available for the most favorable of atomistic data. This ultimately leads …

Exploration Of Intercollegiate Student-Athletes’ Athletic Identity, Leah C. Oldham

Graduate Theses, Dissertations, and Problem Reports (ETD)

Student-athletes encounter experiences that can cause their identity process and self-concept to be disturbed. Even though athletic identities are strong among intercollegiate athletes, some encounter identity-threatening events that interrupt their identity process, negatively affecting their athletic identity. The present study adds to current research on the athletic identity of student-athletes by examining the athletic identity and self-esteem of student-athletes to determine if a redshirt season is a positive (identity-enhancing) or negative (identity-threatening) event. The intersectionality of age, race, gender, and ethnicity are examined to understand if multiple identities influence the experience of a redshirt season. Results reveal that a redshirt …

Aerodynamic Analysis Of Damage State Missiles Using Overset Meshing Techniques For Application To Computational Fluid Dynamics Simulation, Jonathan A. D'Alessio

Graduate Theses, Dissertations, and Problem Reports (ETD)

Significant research over the years has aimed to redefine the flight capabilities of aircraft after sustaining structural damage to critical components. Flight survivability and controllability are key areas of concern when designing fault-tolerant flight control systems to handle a wide range of potential scenarios. However, there is a lack of research on the impact of physically damaged missile systems. Missile counter-defense and intercept capabilities have become more advanced as the United States has focused heavily on these areas over the years. Damaged missiles due to a kinetic intercept capability present an opportunity for analysis of the post-damage implications on flight …

Roles And Responsibilities Of A Coach Developer In A Youth Soccer Setting In The United States, Christina Ann Villalon

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although coaching has a long history guided in the apprenticeship or mentorship model (Taylor & Garratt, 2013), research has primarily focused on the athlete, rarely on the coach, and almost never on whom the coach is serving as an apprentice to, or being mentored or supported by. Internationally, this role has been termed a ‘coach developer,’ but the formalized title and role of the coach developer is still a rather new concept (ICCE, 2014), with most research focused on the coach developer working at elite levels. Therefore, the purpose of this study was to explore the roles and responsibilities of …

Wetland Mitigation Performance Standards: The Intersection Of Science, Practice, And Policy, Jessica Anne Bryzek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Wetland ecosystem spatial extent and integrity have declined due to conversion to anthropogenic land uses. Wetland mitigation offsets the impact on wetlands encountered during development by restoring a previously degraded wetland or creating new wetlands. This activity creates an ecosystem service market where wetland impacts (debits) are offset by wetland restoration/creation (credits). Mitigation aims to replace functions provided by aquatic resources lost during development. Post-restoration monitoring criteria known as performance standards are used to evaluate and verify restored wetland functionality and regulate mitigation credit release. Therefore, performance standards are vital in ascertaining wetland restoration techniques and mitigation processes. In Chapter …

An Action Research Evaluation Of An Online Inclusive Sport Peer Coach Training, Margaret Roberts Condon

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Trained peer tutors are a proven pedagogical technique that can help eliminate barriers to inclusion in physical education and extracurricular sport, including a reported lack of training and experience of professionals. When adequately trained, peer tutors can encourage social interactions, provide constant feedback, and increase active learning and engagement. While recommendations for training peer tutors have been provided, there are currently no known studies examining a peer tutor training in an online format for middle and high school-aged students participating in an inclusive sport program.

Purpose: The purpose of this study was to evaluate the effectiveness of an …

Incentive Analysis Of Blockchain Technology, Rahul Reddy Annareddy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Blockchain technology was invented in the Bitcoin whitepaper released in 2008. Since then, several decentralized cryptocurrencies and applications have become mainstream. There has been an immense amount of engineering effort put into developing blockchain networks. Relatively few projects backed by blockchain technology have succeeded and maintained a large community of developers, users, and customers, while many popular projects with billions of dollars in funding and market capitalizations have turned out to be complete scams.

This thesis discusses the technological innovations introduced in the Bitcoin whitepaper and the following work of the last fifteen years that has enabled blockchain technology. A …

A Novel Tool To Promote Surgical Team Awareness Of Nurse Anesthesia Students’ Level Of Training, Joseph Lewis Britz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Communication errors in operating room (OR) settings are a common and avoidable source of patient harm. Communication in OR settings is enhanced when all members of the surgical team have knowledge of the skills and proficiency of their fellow team members. Student registered nurse anesthetists (SRNAs) provide anesthesia under the supervision of a certified registered nurse anesthetist (CRNA) or physician anesthesiologist. SRNAs’ proficiency in delivering anesthesia can vary significantly depending on their level of training, which varies based on the progression of the student in their multi-year nurse anesthesia program. Methods: This quality improvement project implemented a standardized non-verbal …

Love At First Profile: An Experiment Exploring If Previously Incarcerated Individuals Are Less Desirable While Online Dating, Carina L. Perrone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Incarceration is a rapidly increasing occurrence in the United States with more than 1.4 million people incarcerated and more than 2 million on some form of restricted confinement. While men are incarcerated at a much higher rate than women, women are still at a steady incline of incarceration as well. Also, people of color disproportionately make up a large number of those incarcerated while accounting for a small percentage of the general population. Previous research on people who have been incarcerated focuses on it affects education, employment, and marriage. Little research has attempted to explore how incarceration effects dating, a …

Replication Of A Nonsequential Renewal Model And Investigation Of Extinction Cues For Attenuating Renewal, Brianna G. Sarno

Graduate Theses, Dissertations, and Problem Reports (ETD)

Laboratory models of renewal are critical to inform clinical practice to reduce the occurrence of relapse and facilitate the maintenance of treatment gains. Nonsequential renewal is a modified procedure developed by Sullivan et al. (2018) that provides an alternative arrangement to study operant renewal in a manner more consistent with clinical experience. Experiment 1 replicated the procedure of Craig et al. (2019) to compare renewal of target responding in rats that were exposed to the nonsequential or sequential renewal procedure. Experiment 2 investigated the use of an olfactory extinction cue in mitigating renewal. In Experiment 1, the Nonsequential Group displayed …

The Business Of Behavior: Appalachian Teachers’ Experiences In Using Positive Behavior Interventions And Support, Erin Michele Coffield

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the lived experiences of Appalachian elementary and middle school teachers who have been identified as working in Positive Behavior Intervention and Support (PBIS) schools. This study used Lifeworld Theory to engage in discussion about the teachers’ individual experiences in using PBIS in their classrooms in relation to resilience frameworks and childhood trauma. The teachers’ individual experiences were analyzed using a Whole-Part-Whole analysis to bring the individual stories together to better understand how PBIS and childhood trauma are experienced in a specific Appalachian school. Discussion opens up through the participants' experiences about grit models in school, trauma their …