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Antiviral And Cytotoxic Activities And Chemical Profiles Of Two Species Of Abies Nordmanniana From Türkiye, Nazli Sarikahya, Gaye Sümer Okkali, Furkan Ozan Çöven, Sevda Zeinali̇, Gülce Gucur, Ayşe Di̇la Çağlar, Ece Uyar, Ahmet Ceyhan Gören, Ayşe Nalbantsoy

Turkish Journal of Chemistry

Abies is an important genus of the family Pinaceae, with about 50 species found in the highlands of Asia, Europe, North Africa, and North and Central America. The principal aim of the present work was to investigate the chemical content and biological potential of the resin and cone from Abies nordmanniana subsp. bornmulleriana and Abies nordmanniana subsp. equi-trojani, respectively. The flavonoid and phenolic contents of the resin and cones were evaluated using liquid chromatography-high resolution mass spectrometry (LC-HRMS). Additionally, the essential oil and fatty acid compositions were analyzed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID).. Cytotoxicity …

Assessing The Impact Of Storage Conditions On Rna From Human Saliva And Its Application To The Identification Of Mrna Biomarkers For Asthma, Poorna Manasa Bhamidimarri, David Fuentes, Laila Salameh, Bassam Mahboub, Rifat Hamoudi

Faculty, Staff and Student Publications

Introduction: Human saliva was used to develop non-invasive liquid biopsy biomarkers to establish saliva as an alternate to blood and plasma in translational research. The present study focused on understanding the impact of sample storage conditions on the extraction of RNA from saliva and the RNA yield, to be applied in clinical diagnosis. In this study, genes related to asthma were used to test the method developed.

Methods: Salivary RNA was extracted from three subjects using the Qiazol® based method and quantified by both spectrophotometric (NanoDrop) and fluorometric (Qubit®) methods. RNA integrity was measured using a bioanalyzer. Quantitative PCR was …

When Helping Hurts: The Association Between Hamilton's Rule And Suicide Risk, Katherine L. O'Connell

Clinical Psychology Dissertations

Emergency telecommunicators are critical as first responders and often experience great reward and distressing consequences of their work. This study investigates the suicide risk among these first responders, drawing on the Eusocial Theory of Suicide, which suggests that individuals may engage in altruistic self-sacrifice after weighing benefits, costs, and relatedness using a mathematical model, Hamilton’s Rule. Proxy variables were used: perceived social support and perceived work cohesion for relatedness, compassion satisfaction for benefit, and compassion fatigue for cost. The study was a non-experimental, cross-sectional self-report survey of predominantly White cisgender heterosexual female emergency telecommunicators (N = 346) in Washington, …

When Helping Hurts: The Association Between Hamilton's Rule And Suicide Risk, Katherine L. O'Connell

Clinical Psychology Dissertations

Emergency telecommunicators are critical as first responders and often experience great reward and distressing consequences of their work. This study investigates the suicide risk among these first responders, drawing on the Eusocial Theory of Suicide, which suggests that individuals may engage in altruistic self-sacrifice after weighing benefits, costs, and relatedness using a mathematical model, Hamilton’s Rule. Proxy variables were used: perceived social support and perceived work cohesion for relatedness, compassion satisfaction for benefit, and compassion fatigue for cost. The study was a non-experimental, cross-sectional self-report survey of predominantly White cisgender heterosexual female emergency telecommunicators (N = 346) in Washington, …

Estrous Cycle And Uterine Weight Of Ovariectomized Menopause Female Rat Models After Treatment With Leucaena Leucocephala (Lam.) De Wit Leaf Extract, Ngurah Intan Wiratmini, Anak Agung Sagung Alit Sukmaningsih, Iriani Setyawati

Folia Medica Indonesiana

Highlights: 1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats. 2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock. Highlights: 1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats. 2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock. Abstract Hormone …

Studies In Natural Product-Inspired Complex Molecule Synthesis: Synthesis Of 26-Norpentacyclic Triterpenoids And Anhydroryanodol, Zachary D. Stempel

Dartmouth College Ph.D Dissertations

Natural products have long served as a stimulus for growth and innovation to advance the field of organic chemistry. This thesis describes efforts in complex molecule synthesis that demonstrate the broad value of programs in natural product total synthesis and natural product-inspired function-oriented synthesis. The first part of this thesis describes a function-oriented synthesis approach to pentacyclic triterpenoids through design of a simplified 26-norpentacyclic triterpenoid platform to promote future medicinal exploration in this area. These studies yielded a divergent asymmetric synthesis of 26-norgermanicol and 26-norlupeol as well as the discovery of a new class of androgen receptor antagonist that is …

Interesting Newspaper Comments About Banding Gulls, C. Brooke Worth

Eastern Bird Banding Association News

No abstract provided.

Phantasus, A Web Application For Visual And Interactive Gene Expression Analysis, Maksim Kleverov, Daria Zenkova, Vladislav Kamenev, Margarita Sablina, Maxim N Artyomov, Alexey A Sergushichev

2020-Current year OA Pubs

Transcriptomic profiling became a standard approach to quantify a cell state, which led to the accumulation of huge amount of public gene expression datasets. However, both reuse of these datasets or analysis of newly generated ones requires significant technical expertise. Here, we present Phantasus: a user-friendly web application for interactive gene expression analysis which provides a streamlined access to more than 96,000 public gene expression datasets, as well as allows analysis of user-uploaded datasets. Phantasus integrates an intuitive and highly interactive JavaScript-based heatmap interface with an ability to run sophisticated R-based analysis methods. Overall Phantasus allows users to go all …

The Status And Distribution Of The Prairie Warbler In Ontario, Anne B. Lambert, Roy B. H. Smith

Ontario Birds

No abstract provided.

Synthesis, Characterization, Thermal Analysis Study And Antioxidant Activity For Some Metal Ions Cr (Iii), Fe (Iii), Mn (Ii) And Pd(Ii) Complexes With Azo Dye Derived From P-Methyl-2-Hydroxybenzaldehyde, Adhraa Ghazi Abdulrazzaq, Abbas Ali Salih Al-Hamdani

Baghdad Science Journal

A new Azo Dye ligand HL was 4-((3-formyl-2-hydroxyphenyl)diazenyl)-N-(5-methylisoxazol-3-yl)benzenesulfonamide,this synthesized ligand was used for complexation with different metal ions like Cr(III), Fe(III), Mn(II) and Pd(II) by using a molar ratio of ligand: metalas 1:1. Resulted compounds were characterized by NMR (1H and 13C), UV–vis spectroscopy, TGA, DSC, FT‐IR, MS, elemental analysis, magnetic moment and molar conductivity studies. The results showed that the geometrical structural were octahedral geometries for the Cr(III), Mn(II) and Fe(III) complexes, square planer for Pd(II) complex. The antioxidant activities of the prepared compounds were assessed by using 1.1‐diphenyl‐2‐picrylhydrazyl as the free radical, and the results showed that the …

Introducing A Novel Innovative Technique For The Recording And Interpretation Of Dynamic Coronary Angiography, Thach Nguyen, Duc Khiem Ngo, Tri Loc Vu, Hien Q. Nguyen, Dat H. Pham, Mihas Kodenchery, Gianluca Rigatelli, Aravinda Nanjundappa, Michael Gibson

School of Medicine Publications

In the study of coronary artery disease (CAD), the mechanism of plaque formation and development is still an important subject for investigation. A limitation of current coronary angiography (CAG) is that it can only show static images of the narrowing of arterial channels without identifying the mechanism of the disease or predicting its progression or regression. To address this limitation, the CAG technique has been modified. The new approach emphasizes identifying and analyzing blood flow patterns, employing methodologies akin to those used by hydraulic engineers for fluid or gas movement through domestic or industrial pipes and pumps. With the new …

(R2070) Poisson-Exponentiated Weibull Distribution: Properties, Applications And Extension, Alphonsa George, Dais George

Applications and Applied Mathematics: An International Journal (AAM)

In this article, we introduce a new member of the Poisson-X family namely, the Poisson-exponentiated Weibull distribution. The statistical as well as the distributional properties of the new distribution are studied, and the performance of the maximum likelihood method of estimation is verified by a simulation study. The flexibility of the distribution is illustrated by a real data set. We develop and study a reliability test plan for the acceptance or rejection of a lot of products submitted for inspection when their lifetimes follow the new distribution. A real data example is also given to illustrate the feasibility of the …

Studies On The Corrosion Of Bronze Artifacts From Sarouq Al-Hadid And Al-Qusais Archeological Sites In Dubai, Uae, Zainab Ali Salmin

Theses

This thesis compares the status of bronze artefacts from two archaeological sites in Dubai (UAE): the Sarouq Al-Hadid site (Stone Age to Early Islamic Period) and the Al- Qusais site (Late Bronze Age to Early Iron Age). The main objective of this research is to understand the two sites' environment and their influence on the corrosion of archaeological bronze artifacts found at the sites. This study uses mCed methods to comprehensively understand the corrosion behavior of bronze objects, depending on their burial environment. The two different sites presented different soil types and rates of corrosivity on the buried bronze artifacts, …

Intra- And Interannual Variation In The Foraging Behavior Of Common Murres (Uria Aalge) In The Central California Current, Sean Gee, Pete Warzybok, Michael E. Johns, Jaime Jahncke, Scott A. Shaffer

Faculty Research, Scholarly, and Creative Activity

Seabirds modify their behavior during the breeding season as they transition from incubation (self-feeding) to chick-rearing (self-feeding + chick provisioning) periods, which affects parental foraging patterns and distribution. However, it is less clear how much flexibility parents have to modulate their behavior when environmental conditions are unusually poor. Using GPS loggers and time depth recorders, we examined the foraging flexibility of common murres (Uria aalge) at Southeast Farallon Island as adjustments were made between breeding phases and across consecutive years (2019 and 2020) with contrasting oceanographic conditions (i.e. prominent marine heatwave in 2019). Foraging trips were longer during the incubation …

Molecular-Level Studies Of Nanopatterned Biomolecules With Atomic Force Microscopy, Ashley R. Walker

LSU Doctoral Dissertations

Atomic force microscopy (AFM) is an analytical technique in which a tipped probe is gently scanned across the surface in a raster pattern to generate digital images of a sample at the nanoscale. The AFM instrument has three general operational modes, which are contact, non-contact and tapping-mode, that can be used to examine materials at the atomic level. Single-molecular details of biological molecules and other soft organic materials can be captured with minimal denaturation in either ambient or liquid environments when using tapping-mode AFM. In tapping-mode, the probe is driven to oscillate vertically while the tip is scanned across the …

Nanobodies In The Fight Against Infectious Diseases: Repurposing Nature's Tiny Weapons, Dina Mohamed Moustafa, Soha S. Rizk, Shahira A. Elbanna, Hanzada Tawfik Nour El-Din, Ahmed Sherif Attia

Dentistry

Nanobodies are the smallest known antigen-binding molecules to date. Their small size, good tissue penetration, high stability and solubility, ease of expression, refolding ability, and negligible immunogenicity in the human body have granted them excellence over conventional antibodies. Those exceptional attributes of nanobodies make them promising candidates for various applications in biotechnology, medicine, protein engineering, structural biology, food, and agriculture. This review presents an overview of their structure, development methods, advantages, possible challenges, and applications with special emphasis on infectious diseases-related ones. A showcase of how nanobodies can be harnessed for applications including neutralization of viruses and combating antibiotic-resistant bacteria …

Rapid Identification Of Drug Resistance Of Escherichia Coli Based On Electrochemical Square Wave Voltammetry Of Resazurin, Qi Chuchu, Lu Lisheng, Ren Jiali, Zhou Kai, Zhang Xinfang, Zhang Ziying

Food and Machinery

Objective: To evaluate the antibiotic resistance of five foodborne Escherichia coli clinical isolates to ciprofloxacin, gentamicin and ampicillin. Methods: Based on the theory of electron transfer during bacterial energy metabolism and the introduction of resazurin as a redox probe, an electrochemical square wave voltammetry （SWV） method based on bladed tensin-mediated rapid identification of E. coli drug resistance was established. Results: The electrochemical square wave voltammetry values of E. coli at different concentrations mediated by resazurin were determined, and there was a good linear relationship between the E. coli concentration and the oxidized peak current （I） of resazurin in …

Conference Speaker Bios, Dusty Layton, Angela Williams

Conference Documents

No abstract provided.

Rabies Management In The North, A One Health Problem In A Systems Theory Framework, Karsten Hueffer

Arctic and Northern Studies

Rabies is a significant public health concern and is often described as the quintessential One Health problem, linking especially animal health to human health. I examine how rabies is managed in the circumpolar North with three cases: Alaska, Northwest Territories, and Svalbard. In this thesis, I describe rabies management in the North using a systems theory framework and explore challenges in this limited application of the One Health paradigm, focusing on animal and human health agencies and their interaction in rabies management in a northern environment. I conducted semi-structured interviews with fifteen key informants. My results indicate that most respondents …

In Vitro And In Vivo Burn Healing Study Of Standardized Propolis: Unveiling Its Antibacterial, Antioxidant And Anti-Inflammatory Actions In Relation To Its Phytochemical Profiling, Dina M. El-Kersh, Rania F. Abou El-Ezz, Eman Ramadan, Reham F. El-Kased

Pharmacy

Background

Natural propolis has been used since decades owing to its broad-spectrum activities. Burn injuries are a global health problem with negative impacts on communities. Bacterial infections usually accompany burns, which demand implementation of antibiotics. Antibiotics abuse led to emergence of microbial drug resistance resulting in poor treatment outcomes. In such instances, the promising alternative would be natural antimicrobials such as propolis.

Objective

Full chemical profiling of propolis and evaluation of in vitro antibacterial, antioxidant and anti-inflammatory activities as well as in vivo burn healing properties.

Methods

Chemical profiling of propolis was performed using Liquid chromatography (UHPLC/MS-PDA and HPLC-PDA). In …

Unrestricted Versus Regulated Open Data Governance: A Bibliometric Comparison Of Sars-Cov-2 Nucleotide Sequence Databases, Nathanael Sheehan, Federico Botta, Sabina Leonelli

Copyright, Fair Use, Scholarly Communication, etc.

Two distinct modes of data governance have emerged in accessing and reusing viral data pertaining to COVID-19: an unrestricted model, espoused by data repositories part of the International Nucleotide Sequence Database Collaboration and a regulated model promoted by the Global Initiative on Sharing All Influenza data. In this paper, we focus on publications mentioning either infrastructure in the period between January 2020 and January 2023, thus capturing a period of acute response to the COVID-19 pandemic. Through a variety of bibliometric and network science methods, we compare the extent to which either data infrastructure facilitated collaboration from different countries around …

Characterizing The Expression Of The Sphingosine 1-Phosphate Pathway In Injury And Post-Traumatic Fibrosis, Nicole Cubbage

Masters Theses, 2020-current

Following injury, tissue repair allows for the restoration of the architecture and function of the injured tissue, while tissue replacement is characterized by severely damaged or non-regenerable tissue that is repaired by the laying down of extensive connective tissue, also known as fibrosis. The fibrotic process should be arrested at its early stage to have the greatest impact on preventing or reversing associate sequelae and promoting the regeneration of normal tissue. Sphingosine 1-phosphate (S1P), a metabolic product of cell membrane sphingolipids, has emerged as a pathway that may be involved in fibrosis. Most of S1P’s actions are mediated through the …

Updates To The Alliance Of Genome Resources Central Infrastructure., The Alliance Of Genome Resources Consortium

Faculty Research 2024

The Alliance of Genome Resources (Alliance) is an extensible coalition of knowledgebases focused on the genetics and genomics of intensively studied model organisms. The Alliance is organized as individual knowledge centers with strong connections to their research communities and a centralized software infrastructure, discussed here. Model organisms currently represented in the Alliance are budding yeast, Caenorhabditis elegans, Drosophila, zebrafish, frog, laboratory mouse, laboratory rat, and the Gene Ontology Consortium. The project is in a rapid development phase to harmonize knowledge, store it, analyze it, and present it to the community through a web portal, direct downloads, and application programming interfaces …

Analysis Of Wingtip Vortex Structure Generated By Bio-Inspired Winglets, D'Zahn A. Smith

Doctoral Dissertations and Master's Theses

Bio-inspired winglets were studied with the goal of better understanding the structure of the wingtip vortices they generate. Measurements of the tip vortex were previously carried out using stereoscopic particle image velocimetry (sPIV). The bio-inspired winglet was tested on a rectangular NACA 0012 wing section at a 5-degree angle of attack and at a chord Reynolds number of 900,000. Velocity field measurements were recorded at 0.7 and 2 chord lengths aft of the wing’s trailing edge. Compared to the wing with no attachment and a traditional blended winglet, the bio-inspired winglet’s vortex exhibited significantly less intense flow field properties. These …

Theory And Tools: The Future Of Technology-Based Learning (Script), Andrew S. Gibbons Iii

Faculty Publications

We are entering a narrow passage that will see great instability in learning technology, instruction, and distance education. My purpose is to talk about two changes that I believe will redirect the entire future of technology-based education.

Theory And Tools: The Future Of Technology-Based Learning (Slides), Andrew S. Gibbons Iii

Faculty Publications

We are entering a narrow passage that will see great instability in learning technology, instruction, and distance education. My purpose is to talk about two changes that I believe will redirect the entire future of technology-based education.

Neuroepigenetic Mechanisms Of Brain Development: From Technology To Biological Insights, Allen Yen

Arts & Sciences Graduate Student Theses and Dissertations

Each cell in the brain has the same genomic sequence, yet they can have vastly different phenotypes and function. This diversity is a result of complex genetic and signaling pathways, and knowing how these are regulated is key to understanding physiological development and how pathogenic dysfunctions arise. Genomic methods such as chromatin immunoprecipitation followed by sequencing (ChIP-seq) and assay for transposase-accessible chromatin with sequencing (ATAC-seq) have played pivotal roles in dissecting these processes. However, a key limitation is that the cells are destroyed upon observation of their molecular states, which preclude our ability to correlate historical epigenetic information with future …

Searching For Regulators Of Catalase Activity Using Enzymatic Screens, Aram Joseph Alaxanian

Legacy Theses & Dissertations (2009 - 2024)

Catalase is found in a plethora of organisms and plays a crucial role in safeguarding cells against oxidative stress-induced damage to proteins, RNA, and DNA. Catalase catalyzed the decomposition of hydrogen peroxide (H2O2) into oxygen and water. Deficiencies in catalase have been linked to pathological conditions such as Diabetes, Alzheimer's, and Cancer, underscoring its significance in cellular health. In bacteria like Escherichia coli, catalase is encoded by two genes, KatG and KatE, both subject to regulation through transcription, translation, and post-translational modification. Our study focused on assessing the catalase activity of mutants lacking stress response genes (e.g., lexA, soxR, soxS, …

Role Of M6a Rna Methylation In Skeletal Muscle Regeneration, Isreal Ayobami Onifade

Legacy Theses & Dissertations (2009 - 2024)

Skeletal muscle is essential in orchestrating voluntary movement and contributes to approximately half of the body's overall mass. Dysregulation in proper skeletal muscle development can lead to muscle degenerative disorders, such as severe Duchenne Muscular Dystrophy (DMD). Understanding the process of muscle development has been the central focus of numerous biological studies, focusing on myogenic transcription factors and signaling molecules like Pax3, Pax7, Myf5, MyoD, WNT, TGF-β, and BMP, IGF1, AKT/mTOR pathway. Also, studies have suggested that m6A (N6-methyladenosine) plays a crucial role in various biological processes, including skeletal muscle regeneration and development. m6A modification is the prevalent form of …

Targeting Dendritic Cell Dysfunction To Circumvent Anti-Pd1 Resistance In Head And Neck Cancer, Shin Saito, Michihisa Kono, Hoang C B Nguyen, Ann Marie Egloff, Cameron Messier, Patrick Lizotte, Cloud Paweletz, Douglas Adkins, Ravindra Uppaluri

2020-Current year OA Pubs

PURPOSE: Neoadjuvant anti-PD1 (aPD1) therapies are being explored in surgically resectable head and neck squamous cell carcinoma (HNSCC). Encouraging responses have been observed, but further insights into the mechanisms underlying resistance and approaches to improve responses are needed.

EXPERIMENTAL DESIGN: We integrated data from syngeneic mouse oral carcinoma (MOC) models and neoadjuvant pembrolizumab HNSCC patient tumor RNA-sequencing data to explore the mechanism of aPD1 resistance. Tumors and tumor-draining lymph nodes (DLN) from MOC models were analyzed for antigen-specific priming. CCL5 expression was enforced in an aPD1-resistant model.

RESULTS: An aPD1-resistant mouse model showed poor priming in the tumor DLN due …