Digital Commons Network^™

Intergenerational Trauma, Ruqia Abdirahman

Counselor Education Capstones

The following literature review will explore cumulative research on intergenerational trauma. Although, many communities have encountered massive traumas the research has mainly focused on Canadians First Nations people and Holocaust survivors. The effects of trauma can be multifaceted, impacting an individual’s psychological and emotional well-being across generations. Furthermore, this paper will discuss the impact of intergenerational trauma among various communities, ways trauma transmission occurs, and review of the Intergenerational Trauma Treatment Model.

Usp<800> Compliance: A Hazardous Drug Safe-Handling Quality Improvement Project For Ambulatory Care Infusion Nurses, Cynthia Huff

Doctor of Nursing Practice (DNP) Projects

Problem: The U.S. Pharmacopeia (USP, 2016) Convention Chapter 800: Hazardous Drugs: Handling in Healthcare Settings took effect on December 1, 2019. According to Polovich and Olsen (2017), " The implementation of the USP <800> Standards. will represent an important step forward to protect nurses and other potentially exposed healthcare workers (HCWs): (p.1).

Context: The Doctor of Nursing Practice (DNP) project implemented a hazardous drug (HD) safe-handling personal protective equipment (PPE) toolkit in accordance to USP <800>standards and hospital policies at an ambulatory cancer infusion center to improve nurses' adherence with PPE use.

Interventions: The project consisted of (a) a safe-handling PPE …

What Is The Efficacy Of Biologics For Chronic Plaque-Type Psoriasis Among Pediatric Patients?, Uduakobong Akpainyang

Health Informatics Student Papers and Projects

Chronic plaque psoriasis is an inflammatory skin disease characterized by red thick, raised, silvery scaly patches. It is the most common type of psoriasis, often appearing on the knees, postauricular area, elbows, scalp, and lower back. Chronic plaque psoriasis was the most prevalent (73.7%) among children with psoriasis and was found to increase with age in a study at Olmsted County, Minnesota (Tollefson et al., 2010). Chronic plaque psoriasis is a life-changing condition that significantly impacts the overall health and well-being (emotional, physical, social, and psychological) as well as quality of life in pediatric patients. As a result of data …

3d Cell Culture: Techniques, Applications In Healthcare, And Future Challenges, Mukunthan K S

Manipal Journal of Science and Technology

Two-dimensional (2D) cell cultures have been the most commonly used technique since cell culture was first introduced. However, these cultures do not accurately represent animal physiology as many factors that play an important role in the growth of animal cells are overlooked. Three-dimensional (3D) cell culture techniques provide higher accuracy in recreating the microenvironment of native tissues. But, 2D cell culture techniques are still preferred for the majority of animal cell cultures as these are well established and relatively inexpensive. Various techniques have been developed for 3D cell culture but these systems are highly complex as the number of parameters …

Electron-Rich Alkynes And Azodicarboxylates Chemistry Towards Vicinal Diamine Motif Containing Small Molecules And N-Heterocycles, Chandima Jeewantha Narangoda

All Dissertations

Heterocyclic molecules that contain nitrogens and consist of four, five and six-membered rings such as azetidinones (eg: β-lactams), pyrroles, indoles, azoles, triazoles, oxadiazines, and oxadiazinones, etc. are ubiquitous in the use of pharmaceutical fields. This also reflected in the recently FDA approved unique small-molecule pharmaceuticals, where about 90% of those molecules contain a nitrogen heterocycle. Particularly, the vicinal diamino motif has been appeared in leading drug directing candidates such as penicillin, cephalosporins, and oseltamivir (Tamiflu) over the past years.

Historically, there has been minimal interest in synthesizing a broad scope of vicinal diamino motif-containing small molecules and heterocyclic compounds for …

The Promise Of A Multi-Disciplinary, Mixed-Methods Approach To Inform Insect Pest Management: Evidence From Wyoming Alfalfa, Randa Jabbour, Shiri Noy

Faculty Publications

No abstract provided.

The Application And Development Of Metabolomics Methodologies For The Profiling Of Food And Cellular Toxicity, Jade Woods

Department of Chemistry: Dissertations, Theses, and Student Research

Metabolomics is a rapidly growing field of study. Its growth reflects advancements in technology and an improved understanding of the impact of the environment on metabolism. As a result, metabolomics is now commonly employed to investigate and characterize human and plant metabolism. The first chapter of this thesis provides an introduction to metabolomics and an overview of the protocols for sample preparation, data collection and statistical analysis. The second thesis chapter describes in explicit detail the step-by-step process of extracting and analyzing metabolites collected from mammalian cells, specifically brain tissue with a focus on Parkinson’s disease. The chapter highlights important …

Design, Synthesis And Antibacterial Studies Of Some New Pyridopyrimidine Derivatives As Biotin Carboxylase Inhibitors, Vivek B. Panchabhai, Parag G. Ingole, S R. Butle

Bulletin of Faculty of Pharmacy Cairo University

Present study reports the development of novel pyridopyrimidine derivatives as biotin carboxylase inhibitors with potent antibacterial activity. These compounds were designed to avoid possibility of resistance development. Accordingly eighteen compounds were synthesised and characterized on the basis of spectral data. These compounds were tested for their antibacterial potential by the enzyme kinetic assay against the biotin carboxylase. The minimum inhibitory concentration (MIC) and single step resistance studies were also performed. Compound 2-((2-Phenylpyrido[2,3-d]pyrimidin-4-yl)amino)phenol (6o) showed promising activity in biotin carboxylase inhibition with low MIC. It showed molecular docking score of -7.96, this compound showed formation of hydrogen bonds with the active …

Development Of Computational To Ols To Target Microrna, Luo Song

Dissertations and Theses (Open Access)

MicroRNAs (a.k.a, miRNAs) play an important role in disease development. However, few of their structures have been determined and structure-based computational methods remain challenging in accurately predicting their interactions with small molecules. To address this issue, my thesis is to develop integrated approaches to screening for novel inhibitors by targeting specific structure motifs in miRNAs. The project starts with implementing a tool to find potential miRNA targets with desired motifs. I combined both sequence information of miRNAs and known RNA structure data from Protein Data Bank (PDB) to predict the miRNA structure and identify the motif to target, then I …

Triggerable, Targetable, And Trackable Flavonol-Based Carbon Monoxide-Releasing Molecules, Livia S. Lazarus

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Long known as a toxic gas, carbon monoxide (CO) has been recently redefined as a health beneficial molecule produced endogenously in humans. The biological activity of CO has been extensively examined in inflammatory conditions, vasoprotection, anti-apoptotic and anticancer studies, although the mechanisms of action are still not well defined. Because CO is a gaseous molecule, a straightforward administration would be inhalation. However, this delivery method is limited to use in hospital settings under professional supervision. For improved control over the amount and location of CO delivery, metal-free and light-triggered carbon monoxide-releasing molecules are of particular current interest. Our lab is …

Determining A Method For Expressing And Purifying Cytochrome P450 4v2: A Protein Involved In Bietti's Crystalline Dystrophy, Cody Lane Turner

Graduate Theses/Dissertations

Within the Cytochrome P450 class of enzymes, there are a group known as the “orphan” cytochromes. The “orphan” classification comes from the poorly understood in vivo functionality and substrate specificity. Cytochrome P450 4V2 (CYP4V2) is one of these “orphans” and belongs to the CYP4 family. The CYP4 family is known for the omega oxidation of endogenous fatty acids. This family is most commonly found on chromosome 1 (CYP4ABXZ). CYP4V2 is unique in that its location is bound to chromosome 4 as discovered by Jiao in 2004. Mutations within the CYP4V2 gene have been associated with the …

The Use Of It Tools In The Assessment And Development Of Leadership Abilities, Stanisław Stanek, Agnieszka Sabat

European Management Studies

This paper aims to present the findings of research into the possibilities of deploying IT tools to support the assessment and development of leadership abilities. The research procedure consisted in an analysis of four case studies from the business and military sectors. The primary research methods were a case study analysis, participant observation, Development Centre, and a source literature analysis. Under individual case studies, the following techniques were applied: individual and group interviews, brainstorming, and role playing. The tools applied included psychometric software (Thomas International, Extended Disc, Thalento) and military simulators. The division of roles between man and computer was …

Plant Single-Cell Metabolomics—Challenges And Perspectives, Leonardo Perez De Souza, Monica Borghi, Alisdair R. Fernie

Biology Faculty Publications

Omics approaches for investigating biological systems were introduced in the mid-1990s and quickly consolidated to become a fundamental pillar of modern biology. The idea of measuring the whole complement of genes, transcripts, proteins, and metabolites has since become widespread and routinely adopted in the pursuit of an infinity of scientific questions. Incremental improvements over technical aspects such as sampling, sensitivity, cost, and throughput pushed even further the boundaries of what these techniques can achieve. In this context, single-cell genomics and transcriptomics quickly became a well-established tool to answer fundamental questions challenging to assess at a whole tissue level. Following a …

Posttranslational Modification And Protein Disorder Regulate Protein-Protein Interactions And Dna Binding Specificity Of P53, Robin Levy

USF Tampa Graduate Theses and Dissertations

p53 is an intrinsically disordered transcription factor that suppresses tumor development by arresting the cell cycle and promoting DNA repair. p53 deletions or mutations can lead to cancer due to the inability of cells to respond to stress. The protein levels and post-translational modification state of p53 changes in response to cellular stress like DNA damage. Previous studies have shown that p53 can undergo coupled folding and binding with the E3 ubiquitin ligase, Mdm2, and the histone deacetylase, p300. In normal cells, p53 is kept at a low level by Mdm2, which marks it with ubiquitin, targeting p53 for proteasome …

Design Of A Comprehensive Modeling, Characterization, Rupture Risk Assessment And Visualization Pipeline For Abdominal Aortic Aneurysms, Golnaz Jalalahmadi

Theses

Abdominal aortic aneurysms (AAA) is a dilation of the abdominal aorta, typically within the infra-renal segment of the vessel that cause an expansion of at least 1.5 times the normal vessel diameter. It is becoming a leading cause of death in the United States and around the world, and consequentially, in 2009, the Society for Vascular Surgery (SVS) practice guidelines expressed the critical need to further investigate the factors associated with the risk of AAA rupture, along with potential treatment methods. For decades, the maximum diameter (Dmax) was introduced as the main parameter used to assess AAA behavior and its …

Lysenin Channels As Sensors For Ions And Molecules, Andrew Bogard, Gamid Abatchev, Zoe Hutchinson, Jason Ward, Pangaea W. Finn, Fulton Mckinney, Daniel Fologea

Physics Faculty Publications and Presentations

Lysenin is a pore-forming protein extracted from the earthworm Eisenia fetida, which inserts large conductance pores in artificial and natural lipid membranes containing sphingomyelin. Its cytolytic and hemolytic activity is rather indicative of a pore-forming toxin; however, lysenin channels present intricate regulatory features manifested as a reduction in conductance upon exposure to multivalent ions. Lysenin pores also present a large unobstructed channel, which enables the translocation of analytes, such as short DNA and peptide molecules, driven by electrochemical gradients. These important features of lysenin channels provide opportunities for using them as sensors for a large variety of applications. In …

Determination Of Vitamin D Metabolites And Their Epimers In Obese Emirati Population, Bashar Hiatham Alzohily

Theses

Vitamin D deficiency is a global issue, and despite all-year-round sunshine, it is a big problem in the UAE. Obesity or overweight refers to an accumulation of excessive fat that may harm health so, it is considered a worldwide health concern. The accurate measurement of vitamin D active forms is a problematic issue. This study aimed to develop and validate a UHPLC-MS/MS method for the new method for determining vitamin D levels in the obese Emirati population. The vitamin D epimers and other metabolites were determined by UHPLC-MS/MS system, model 8060. The methods include the collection of blood samples from …

The Role Of Emotional Intelligence In Chaplaincy: An Analysis If Ei In The Delivery Of Patient-Centered Care, Dean A. Darroux

Doctoral Dissertations and Projects

This study addressed the role of emotional intelligence among healthcare chaplains and its place in delivering patient-centered care at Adventist healthcare, Maryland. The study assumed that since healthcare chaplains’ journeys with their patients help them process their feelings as they understand the nature of their illness, they must identify their feelings and that of their patients to provide better care. It further examined the role of personality in determining its relationship with emotional intelligence and how it would influence patient-centered care. The literature surveyed indicated that emotional intelligence helps individuals understand their emotions as well as that of others. A …

Morphologic Remission Status Is Limited Compared To Δn Flow Cytometry: A Children's Oncology Group Aaml0531 Report., Lisa Eidenschink Brodersen, Robert B. Gerbing, M Laura Pardo, Todd A. Alonzo, Dana Paine, Wayne Fritschle, Fan-Chi Hsu, Jessica A. Pollard, Richard Aplenc, Samir B. Kahwash, Betsy Hirsch, Susana Ramondi, Denise Wells, E Anders Kolb, Alan S. Gamis, Soheil Meshinchi, Michael R. Loken

Manuscripts, Articles, Book Chapters and Other Papers

Risk stratification for acute myeloid leukemia (AML) uses molecular and cytogenetic abnormalities identified at diagnosis. Response to therapy informs risk, and morphology continues to be used more frequently than flow cytometry. Herein, the largest cohort of pediatric patients prospectively assessed for measurable residual disease (MRD) by flow cytometry (N = 784) is reported. The "difference from normal" (ΔN) technique was applied: 31% of all patients tested positive (AML range, 0.02% to 91%) after the first course of treatment on Children's Oncology Group study AAML0531. Detection of MRD following initial chemotherapy proved the strongest predicator of overall survival (OS) in univariable …

Chemical Synthesis Of Homogeneous Human E-Cadherin N-Linked Glycopeptides: Stereoselective Convergent Glycosylation And Chemoselective Solid-Phase Aspartylation, Chen Zeng, Bin Sun, Xuefeng Cao, Joshua Zhu, Olawale Micheal Oluwadahunsi, Ding Liu, He Zhu, Jiabin Zhang, Qing Zhang, Gaolan Zhang, Christopher Andrew Gibbons, Yunpeng Liu, Jun Zhou, Peng George Wang

Faculty Work Comprehensive List

We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide. The key steps are a chemoselective on-resin aspartylation using a pseudoproline-containing peptide and stereoselective glycosylation using glycosyl fluororide as a donor. This synthetic strategy demonstrates potential utility in accessing a wide range of homogeneous N-linked glycopeptides for the examination of their biological function.

Genetically Encoded Live-Cell Sensor For Tyrosinated Microtubules, Shubham Kesarwani, Prakash Lama, Anchal Chandra, P. Purushotam Reddy

Open Access archive

Microtubule cytoskeleton exists in various biochemical forms in different cells due to tubulin posttranslational modifications (PTMs). Tubulin PTMs are known to affect microtubule stability, dynamics, and interaction with MAPs and motors in a specific manner, widely known as tubulin code hypothesis. At present, there exists no tool that can specifically mark tubulin PTMs in living cells, thus severely limiting our understanding of their dynamics and cellular functions. Using a yeast display library, we identified a binder against terminal tyrosine of α-tubulin, a unique PTM site. Extensive characterization validates the robustness and nonperturbing nature of our binder as tyrosination sensor, a …

Expression, Purification, And Glycosylation Of Epidermal Growth Factor-Like Repeat 27 From Mouse Notch1, Justin A. Grennell, Kendra D. Jenkins, Huimin Zhong, Amid Paudyal, Kelvin B. Luther, Robert S. Haltiwanger, Megan A. Macnaughtan

Faculty Publications

© 2020 Elsevier Inc. Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells. Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions. Each EGF repeat consists of about 40 amino acids with 3 conserved disulfide bonds. Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat. EGF repeat 27 (EGF27) from mouse NOTCH1 is …

The Role Of System Xc- In Cognition: The Importance Of Neuron-Astrocyte Signaling, Evan Michael Hess

Dissertations (1934 -)

The biological basis of human intelligence is largely a mystery, but likely required evolutionary adaptations to achieve the information processing capacity needed to expand the complexity of cognition among species. The link between evolutionary expansion of signaling complexity in the brain and cognition has largely focused on neuronal mechanisms, in part because information processing has historically been attributed to these cells. However, astrocytes are emerging as a second type of brain cell that is capable of processing information due to their capacity to release glutamate and, thereby, regulate neural circuits. Hence, a modern question is whether astrocytes contributed to the …

Ampk Regulation Of Raptor And Tsc2 Mediate Metformin Effects On Transcriptional Control Of Anabolism And Inflammation, Jeanine L Van Nostrand, Kristina Hellberg, En-Ching Luo, Eric L Van Nostrand, Alina Dayn, Jingting Yu, Maxim N Shokhirev, Yelena Dayn, Gene W Yeo, Reuben J Shaw

Faculty, Staff and Students Publications

Despite being the frontline therapy for type 2 diabetes, the mechanisms of action of the biguanide drug metformin are still being discovered. In particular, the detailed molecular interplays between the AMPK and the mTORC1 pathway in the hepatic benefits of metformin are still ill defined. Metformin-dependent activation of AMPK classically inhibits mTORC1 via TSC/RHEB, but several lines of evidence suggest additional mechanisms at play in metformin inhibition of mTORC1. Here we investigated the role of direct AMPK-mediated serine phosphorylation of RAPTOR in a new

Age And Apoe Genotype Affect The Relationship Between Objectively Measured Physical Activity And Power In The Alpha Band, A Marker Of Brain Disease, Jaisalmer De Frutos-Lucas, Pablo Cuesta, Federico Ramírez-Toraño, Alberto Nebreda, Esther Cuadrado-Soto, África Peral-Suárez, David Lopez-Sanz, Ricardo Bruña, Silvia Marcos-De Pedro, María Luisa Delgado-Losada, Ana María López-Sobaler, Inmaculada Concepción Rodríguez-Rojo, Ana Barabash, Juan Manuel Serrano Rodriguez, Simon M. Laws, Alberto Marcos Dolado, Ramón López-Higes, Belinda M. Brown, Fernando Maestú

Research outputs 2014 to 2021

BACKGROUND: Electrophysiological studies show that reductions in power within the alpha band are associated with the Alzheimer's disease (AD) continuum. Physical activity (PA) is a protective factor that has proved to reduce AD risk and pathological brain burden. Previous research has confirmed that exercise increases power in the alpha range. However, little is known regarding whether other non-modifiable risk factors for AD, such as increased age or APOE ε4 carriage, alter the association between PA and power in the alpha band. METHODS: The relationship between PA and alpha band power was examined in a sample of 113 healthy adults using …

Biological Sciences Graduate Society Makes Plans To Keep Community Engaged, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Point-Of-Care Devices For Therapeutic, Medical And Environmental Applications, Alisha Prasad

LSU Doctoral Dissertations

Point-of-care testing (POCT) or Point-of-use (POU) devices or technologies are defined as testing aids that are capable for onsite use or testing. The key advantages of POCT are low sample volume, quick onsite diagnosis, high accuracy, and cost-effectiveness. POCT has the potential and the benefits to facilitate better health care management by rapid routine diagnosis and monitoring. To reach this goal, several researchers as well as the healthcare industry over a few years have conducted cutting edge research to bring science to technology by developing smart diagnostic devices capable of performing as per patient profiles and make personalized health care …

Wyatt, Alumnus Head To Kennedy Space Center, Prepare For Next Space Flight, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Glycomics: Immunoglobulin Gn-Glycosylation Associated With Mammary Gland Hyperplasia In Women, Zixiu Meng, Cancan Li, Guoyong Ding, Weijie Cao, Xizhu Xu, Yuanyuan Heng, Yang Deng, Yuejin Li, Xiaoyu Zhang, Dong Li, Wei Wang, Youxin Wang, Weijia Xing, Haifeng Hou

Research outputs 2014 to 2021

Mammary gland hyperplasia (MGH) is very common, especially among young and middle-aged women. New diagnostics and biomarkers for MGH are needed for rational clinical management and precision medicine. We report, in this study, new findings using a glycomics approach, with a focus on immunoglobulin G (IgG) N-glycosylation. A cross-sectional study was conducted in a community-based population sample in Beijing, China. A total of 387 participants 40-65 years of age were enrolled in this study, including 194 women with MGH (cases) and 193 women who had no MGH (controls). IgG N-glycans were characterized in the serum by ultra-performance liquid chromatography. The …

A Comparative Study On The Active Constituents, Antioxidant Capacity And Anti-Cancer Activity Of Cruciferous Vegetable Residues, Alaa A. Gaafar, Zeinab A. Salama, Farouk K. El-Baz

Baghdad Science Journal

This study is pointed out to estimate the effectiveness of two solvents in the extraction and evaluating the active ingredients and their antioxidant activity as well as anti-cancer efficiency. Therefore, residues from four different Brassica vegetables viz. broccoli, Brussels sprout, cauliflower, and red cherry radish were extracted using two procedures methods: methanolic and water crude extracts. Methanol extracts showed the highest content of total phenolic (TP), total flavonoids (TF), and total tannins (TT) for broccoli and Brussels sprouts residues. Methanolic extract of broccoli and Brussels sprouts residues showed the highest DPPH· scavenging activity (IC50 = 15.39 and 18.64 µg/ml). The …