Digital Commons Network^™

Svep1 Is An Endogenous Ligand For The Orphan Receptor Pear1, Jared S Elenbaas, Upasana Pudupakkam, Katrina J Ashworth, Chul Joo Kang, Ved Patel, Katherine Santana, In-Hyuk Jung, Paul C Lee, Kendall H Burks, Junedh M Amrute, Robert P Mecham, Carmen M Halabi, Arturo Aliso, Jorge Di Paola, Nathan O Stitziel

2020-Current year OA Pubs

Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1 (SVEP1) is an extracellular matrix protein that causally promotes vascular disease and associates with platelet reactivity in humans. Here, using a human genomic and proteomic approach, we identify a high affinity, disease-relevant, and potentially targetable interaction between SVEP1 and the orphan receptor Platelet and Endothelial Aggregation Receptor 1 (PEAR1). This interaction promotes PEAR1 phosphorylation and disease associated AKT/mTOR signaling in vascular cells and platelets. Mice lacking SVEP1 have reduced platelet activation, and exogenous SVEP1 induces PEAR1-dependent activation of platelets. SVEP1 and PEAR1 causally and concordantly relate to platelet …

Developmental Programming: Adverse Sexually Dimorphic Transcriptional Programming Of Gestational Testosterone Excess In Cardiac Left Ventricle Of Fetal Sheep, Venkateswaran Ramamoorthi Elangovan, Nadia Saadat, Adel Ghnenis, Vasantha Padmanabhan, Arpita K Vyas

2020-Current year OA Pubs

Adverse in-utero insults during fetal life alters offspring's developmental trajectory, including that of the cardiovascular system. Gestational hyperandrogenism is once such adverse in-utero insult. Gestational testosterone (T)-treatment, an environment of gestational hyperandrogenism, manifests as hypertension and pathological left ventricular (LV) remodeling in adult ovine offspring. Furthermore, sexual dimorphism is noted in cardiomyocyte number and morphology in fetal life and at birth. This study investigated transcriptional changes and potential biomarkers of prenatal T excess-induced adverse cardiac programming. Genome-wide coding and non-coding (nc) RNA expression were compared between prenatal T-treated (T propionate 100 mg intramuscular twice weekly from days 30 to 90 …

Fmr1 Mutation Alters The Early Development Of Sensory Coding And Hunting And Social Behaviors In Larval Zebrafish, Shuyu I Zhu, Michael H Mccullough, Zac Pujic, Jordan Sibberas, Biao Sun, Thomas Darveniza, Bianca Bucknall, Lilach Avitan, Geoffrey J Goodhill

2020-Current year OA Pubs

Autism spectrum disorders (ASDs) are developmental in origin; however, little is known about how they affect the early development of behavior and sensory coding. The most common inherited form of autism is Fragile X syndrome (FXS), caused by a mutation in

Intestinal Neuropod Cell Gucy2c Regulates Visceral Pain, Joshua R. Barton, Annie K. Londregran, Tyler D. Alexander, Ariana A. Entezari, Shely Bar-Ad, Lan Cheng, Angelo C. Lepore, Adam E. Snook, Manuel Covarrubias, Scott A. Waldman

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Visceral pain (VP) is a global problem with complex etiologies and limited therapeutic options. Guanylyl cyclase C (GUCY2C), an intestinal receptor producing cyclic GMP(cGMP), which regulates luminal fluid secretion, has emerged as a therapeutic target for VP. Indeed, FDA-approved GUCY2C agonists ameliorate VP in patients with chronic constipation syndromes, although analgesic mechanisms remain obscure. Here, we revealed that intestinal GUCY2C was selectively enriched in neuropod cells, a type of enteroendocrine cell that synapses with submucosal neurons in mice and humans. GUCY2Chi neuropod cells associated with cocultured dorsal root ganglia neurons and induced hyperexcitability, reducing the rheobase and increasing the resulting …

Form Cdc14 Phosphatase Contributes To Cell Wall Integrity And Pathogenesis In Candida Albicans, Kedric L. Milholland, Ahmed Abdelkhalek, Kortany M. Baker, Smriti Hoda, Andrew G. Demarco, Noelle H. Naughton, Angela N. Koeberlein, Gabrielle R. Lorenz, Kartikan Anandasothy, Antonio Esperilla-Muñoz, Sanjeev K K. Narayanan, Jaime Correa-Bordes, Scott D. Briggs, Mark C. Hall

Purdue University Libraries Open Access Publishing Fund

The Cdc14 phosphatase family is highly conserved in fungi. In Saccharomyces cerevisiae, Cdc14 is essential for down-regulation of cyclin-dependent kinase activity at mitotic exit. However, this essential function is not broadly conserved and requires only a small fraction of normal Cdc14 activity. Here, we identified an invariant motif in the disordered C-terminal tail of fungal Cdc14 enzymes that is required for full enzyme activity. Mutation of this motif reduced Cdc14 catalytic rate and provided a tool for studying the biological significance of high Cdc14 activity. A S. cerevisiae strain expressing the reduced-activity hypomorphic mutant allele (cdc14hm ) as the …

Neuroprotective Effects Of Green Tea (Camellia Sinensis) And Broccoli (Brassica Oleracea) Extracts In An In Vitro Model Of Multiple Sclerosis, Sara Taha Fathallah

Theses and Dissertations

Multiple Sclerosis (MS) is a neurodegenerative autoimmune disease in which the immune system attacks the central nervous system (CNS) resulting in demyelination. Currently, there is no specific treatment for MS. Here, we propose testing the neuroprotective potential of Green tea (Camellia sinensis), and Broccoli (Brassica oleracea) extracts in an in vitro model of multiple sclerosis.

Methods and Results: Green tea and Broccoli plant extracts were analyzed with GC-MS and tested for their neuroprotective effects in an in vitro MS model using Cuprizone (CPZ) on the Human neuroblastoma SH-SY5Y cell line. Cell viability assays proved the safety of the extracts …

On The Balcony, Beyond “Balconearing”; Perception Of Cairene Women, Behind The Curtains., Rowida Magdy Al-Gebeily

Theses and Dissertations

This study sheds light on the significance and role of threshold spaces as means of accomplishing sociocultural needs, restoration and wellbeing in the residential environment. Understanding the function and uses of these spaces allows us to appreciate their benefits that are often neglected. The research particularly focuses on the social dimension of one fundamental threshold space; the Cairene balcony. It is an integral space that provides a valuable connection to the outside, and its importance in the development of urban living is widely recognized. If carefully designed to meet the residents’ needs and demands, they can promote a better quality …

Molecular Diagnosis Of Non X-Linked Ectodermal Dysplasias Using Next Generation Sequencing, Eman Abdelalim Rabie

Theses and Dissertations

Ectodermal Dysplasias (EDs) are rare heterogenous monogenic developmental disorders sharing the impairment of at least two surface ectoderm-derived organs. Symptoms characteristically manifest developmental abnormalities of the teeth, and three skin derivatives: hair follicles, nails, and sweat glands. The disease-causing gene was identified for only fewer than half of 160 characterized ED phenotypes. Expectedly, ED-causing genes regulate or function in ectodermal-developmental processes. The most common phenotype is hypohidrotic ED featuring teeth agenesis, and diminished or complete absence of hair and sweat production. Disease-causing variants of EDA, EDAR, EDARADD and WNT10A genes were identified in 60-90% of ED patients in …

Journal Of Shock And Hemodynamics, Vol. I, Iss. 2 (Print Version)

Journal of Shock and Hemodynamics

The print version of Volume I, Issue 2 of the Journal of Shock and Hemodynamics was published in February 2023. The PDF of the print version is downloadable here.

Towards A Responsive Resilient Supply Chain Based On Industry 5.0: A Case Study In Healthcare Systems, Abduallah Gamal, Amal F. Abd El-Gawad, Mohamed Abouhawwash

Neutrosophic Systems with Applications

Executives and academics have presented the idea of Industry 5.0, which is an attempt to build upon the previous iteration, Industry 4.0, by including many important tenets such as human-centricity, resilience, and sustainability. Because of the significance of this idea, the current study provides a decision-making framework to examine a responsive supply chain dubbed responsive supply chain 5.0 for healthcare systems. This framework takes into consideration the aspects of Industry 5.0. In order to do this, at the onset, the most important connected factors and strategies are determined by consulting the relevant experts and body of published research. This problem …

Riluzole And Novel Naphthalenyl Substituted Aminothiazole Derivatives Prevent Acute Neural Excitotoxic Injury In A Rat Model Of Temporal Lobe Epilepsy, Thomas Kyllo, Vikrant Singh, Heesung Shim, Singh Latika, Hai M. Nguyen, Yi Je Chen, Ellen Terry, Heike Wulff, Jeffrey D. Erickson

School of Medicine Faculty Publications

Epileptogenic seizures, or status epilepticus (SE), leads to excitotoxic injury in hippocampal and limbic neurons in the kainic acid (KA) animal model of temporal lobe epilepsy (TLE). Here, we have further characterized neural activity regulated methylaminoisobutryic acid (MeAIB)/glutamine transport activity in mature rat hippocampal neurons in vitro that is inhibited by riluzole (IC50 = 1 μM), an anti-convulsant benzothiazole agent. We screened a library of riluzole derivatives and identified SKA-41 followed by a second screen and synthesized several novel chlorinated aminothiazoles (SKA-377, SKA-378, SKA-379) that are also potent MeAIB transport inhibitors in vitro, and brain penetrant following systemic administration. When …

Ensemble Concerts: Symphonic Winds And Wind Symphony, February 15, 2023, Anthony C. Marinello Iii, Mack Wood, John Gonzalez, Jeason Lopez

School of Music Programs

Center for the Performing Arts
February 15, 2023
Wednesday Evening
8:00 p.m.

Simulation Usage As A Partial Replacement Of Traditional Clinical: A Study Of Administrator And Faculty Perceptions In Practical Nursing Programs In Pennsylvania, Cynthia A. Cornelius

Dissertations

There has been a significant amount of research regarding simulation use in nursing education; however, there is limited research available regarding the replacement of traditional clinical with simulated clinical. This research included a mixed-method design formulated to determine Pennsylvania Practical Nursing Programs' current simulation usage and Pennsylvania Practical Nursing Directors', Assistant Administrators', and Faculty Members' perceptions regarding replacing a portion of traditional clinical with simulated clinical. An 18-item electronic questionnaire was delivered to 309 potential respondents via the Qualtrics electronic survey distribution system, with 191 responding.

Effects Of Date Seed (Phoenix Dactylifera) Extract In An Animal Model Of Diabetes Mellitus, Sara Refaat

Theses and Dissertations

Diabetes mellitus is a chronic illness and a global epidemic. The commercial diabetic medicines available are expensive and have several adverse effects. Alternative natural products are still being sought for. Date seeds ( Phoenix dactylifera) are either thrown away as garbage or utilized as animal feed. The use of seed extract as an alternative therapy for diabetes offers a cost-effective and all-natural method for managing the disease. Methods Sprague dawley rats were randomly distributed into groups with a sample size of 4 rats per group. Date seed powder was boiled producing a 10% date seed extract which was administered in …

Factors Affecting Consumers’ Purchase Intentions Towards Recycled Products, Sherine Esmat

Theses and Dissertations

The work executed addresses the status of recycling industry in Egypt and its relation to the current sustainability trends as well as climate change issues. It also identifies the main concerns of consumers when purchasing recycled products. Consequently, identifies the key pillars that need to be applied or communicated with the target consumers to enhance the concept of selling recycled products and increase its market share. Since this industry is significantly broad, the focus of the study was only on recycled PET plastic bottles (rPET), this is to ensure attaining accurate and reliable results.

An interview and a site visit …

Spartan Daily, February 15, 2023, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2023

Volume 160, Issue 9

Decreasing Mutant Atxn1 Nuclear Localization Improves A Spectrum Of Sca1-Like Phenotypes And Brain Region Transcriptomic Profiles, Hillary P Handler, Lisa Duvick, Jason S Mitchell, Marija Cvetanovic, Molly Reighard, Alyssa Soles, Kathleen B Mather, Orion Rainwater, Shannah Serres, Tessa Nichols-Meade, Stephanie L Coffin, Yun You, Brian L Ruis, Brennon O'Callaghan, Christine Henzler, Huda Y Zoghbi, Harry T Orr

Duncan NRI Faculty and Staff Publications

Spinocerebellar ataxia type 1 (SCA1) is a dominant trinucleotide repeat neurodegenerative disease characterized by motor dysfunction, cognitive impairment, and premature death. Degeneration of cerebellar Purkinje cells is a frequent and prominent pathological feature of SCA1. We previously showed that transport of ATXN1 to Purkinje cell nuclei is required for pathology, where mutant ATXN1 alters transcription. To examine the role of ATXN1 nuclear localization broadly in SCA1-like disease pathogenesis, CRISPR-Cas9 was used to develop a mouse with an amino acid alteration (K772T) in the nuclear localization sequence of the expanded ATXN1 protein. Characterization of these mice indicates that proper nuclear localization …

Lewy Body-Like Pathology And Loss Of Dopaminergic Neurons In Midbrain Organoids Derived From Familial Parkinson’S Disease Patient, Andrea Becerra-Calixto, Abhisek Mukherjee, Santiago Ramirez, Sofia Sepulveda, Tirthankar Sinha, Rabab Al-Lahham, Nicole De Gregorio, Camila Gherardelli, Claudio Soto

Faculty, Staff and Student Publications

Progressive accumulation of α-Synuclein (αSyn) in Lewy bodies (LBs) and loss of dopaminergic (DA) neurons are the hallmark pathological features of Parkinson's disease (PD). Although currently available in vitro and in vivo models have provided crucial information about PD pathogenesis, the mechanistic link between the progressive accumulation of αSyn into LBs and the loss of DA neurons is still unclear. To address this, it is critical to model LB formation and DA neuron loss, the two key neuropathological aspects of PD, in a relevant in vitro system. In this study, we developed a human midbrain-like organoid (hMBO) model of PD. …

Combination Of Epha2- And Wee1-Targeted Therapies In Endometrial Cancer, Santosh K Dasari, Robiya Joseph, Sujanitha Umamaheswaran, Lingegowda S Mangala, Emine Bayraktar, Cristian Rodriguez-Aguayo, Yutuan Wu, Nghi Nguyen, Reid T Powell, Mary Sobieski, Yuan Liu, Mamur A Chowdhury, Paola Amero, Clifford Stephan, Gabriel Lopez-Berestein, Shannon N Westin, Anil K Sood

Faculty, Staff and Student Publications

EphA2 tyrosine kinase is upregulated in many cancers and correlated with poor survival of patients, including those with endometrial cancer. EphA2-targeted drugs have shown modest clinical benefit. To improve the therapeutic response to such drugs, we performed a high-throughput chemical screen to discover novel synergistic partners for EphA2-targeted therapeutics. Our screen identified the Wee1 kinase inhibitor, MK1775, as a synergistic partner to EphA2, and this finding was confirmed using both in vitro and in vivo experiments. We hypothesized that Wee1 inhibition would sensitize cells to EphA2-targeted therapy. Combination treatment decreased cell viability, induced apoptosis, and reduced clonogenic potential in endometrial …

Ush2a Mutation And Specific Driver Mutation Subtypes Are Associated With Clinical Efficacy Of Immune Checkpoint Inhibitors In Lung Cancer, Dexin Yang, Yuqin Feng, Haohua Lu, Kelie Chen, Jinming Xu, Peiwei Li, Tianru Wang, Dajing Xia, Yihua Wu

Faculty, Staff and Student Publications

This study aimed to identify subtypes of genomic variants associated with the efficacy of immune checkpoint inhibitors (ICIs) by conducting systematic literature search in electronic databases up to May 31, 2021. The main outcomes including overall survival (OS), progression-free survival (PFS), objective response rate (ORR), and durable clinical benefit (DCB) were correlated with tumor genomic features. A total of 1546 lung cancer patients with available genomic variation data were included from 14 studies. The Kirsten rat sarcoma viral oncogene homolog

The Bg News February 15, 2023, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. February 15, 2023. Volume 102-Issue 18.

Incentive Mechanisms To Exploit Intraseasonal Price Arbitrage Opportunities For Smallholder Farmers: Experimental Evidence From Malawi, Tabitha Nindi, Jacob Ricker-Gilbert, Jonathan Bauchet

Department of Agricultural Economics Faculty Publications

Seasonal commodity price fluctuations can potentially offer farmers arbitrage opportunities to increase their income. However, smallholder farmers in most of sub-Saharan Africa often do not exploit these opportunities to the fullest extent possible. To inform this issue, we conducted a randomized controlled trial among 1739 smallholder farmers in Malawi to estimate the impact of two key post-harvest constraints, lack of appropriate storage technology and commitment issues, on farmers' legume storage and sales decisions. The treated groups received (i) an improved storage technology in the form of two hermetic (airtight) bags, (ii) the same improved storage technology under the condition that …

The Nazis, The Vatican, And The Jews Of Rome, Patrick J. Gallo

Purdue University Press Books

On October 16, 1943, the Jews of Rome were targeted for arrest and deportation. The Nazis, the Vatican, and the Jews of Rome examines why—and more importantly how—it could have been avoided, featuring new evidence and insight into the Vatican’s involvement. At the time, Rome was within reach of the Allies, but the overwhelming force of the Wehrmacht, Gestapo, and SS in Rome precluded direct confrontation. Moral condemnations would not have worked, nor would direct confrontation by the Italians, Jewish leadership, or even the Vatican.

Gallo underscores the necessity of determining what courses of actions most likely would have spared …

“Tweeting During Emergencies”: The Egyptian Ministry Of Health Twitter Communication Strategy Under The Umbrella Of The Crisis And Emergency Risk Communication Model (Cerc) Case Study: Covid-19 Pandemic, Pakinam Elgohary

Theses and Dissertations

This research investigates using the Crisis and Emergency Risk Communication Model (CERC) for COVID- 19 communication by analyzing the content of Twitter messages posted by the Government of Egypt through the Egyptian Ministry of Health (MOHP). It further examines how official communicators and institutions utilize social media to contact the public during emergencies like the COVID-19 pandemic, highlighting critical strategies of recommendations. Crisis and Emergency Risk Communication (CERC), a five-staged theory, recommends a set of messaging and pertained communication characteristics to implement at each stage of the identified following stages: (1) “Pre-crisis, (2) Initial event, (3) Maintenance, (4) Resolution, and …

Biogas Combustion Characteristics In A Concentric Flow Slot Burner: Effects Of Co2 Concentration On Stability And Flame Structure, Maged Kiriakos

Theses and Dissertations

Biogas combustion is affected by the concentration of carbon dioxide. The successful applications of Biogas as a sustainable renewable alternative fuel produced from waste depend on its combustion stability, heat release, and pollution level. The aim of the current study is to apply new combustion technology and study the stability and combustion characteristics of natural gas with different percentages of carbon dioxide from 0 to 40% simulating biogas fuel. The stability characteristics and the temperature profiles of turbulent planar flames at different levels mixture inhomogeneity are investigated and presented in this work. The flames are created in a newly developed …

Formation Of Local Elite Power Base In Local Politics: “The Emerging” And “The Surviving”, Muhtar Haboddin, La Ode Machdani Afala

Jurnal Politik

Many recent studies show the development of an increasingly diverse elite characterized by the emergence of a new elite and, on the other hand, the collapse of the old elite. However, these studies mainly infer democratic political change as an explanatory factor. This article takes a different standpoint to explain why some local elites collapse, survive, and emerge as new elites. Specifically, this article describes the formation of the elite resource base and its accumulation by emphasizing the perspective of social changes. Resources become the basis of power that determines the position of the elite in society and the political …

Blood Transcriptome Responses To Pfoa And Genx Treatment In The Marsupial Biomedical Model Monodelphis Domestica, Wenqi Cao, Katharine Horzmann, Bettina Schemera, Myra Petrofski, Trisha Kendall, Jennifer Spooner, Patricia E. Rynders, John Vandeberg, Xu Wang

School of Medicine Publications

Introduction: Perfluoroalkyl and poly-fluoroalkyl substances (PFASs) are widely used in industrial and consumer products. Due to their environmental persistence and bioaccumulation, PFASs can be found in the blood of humans and wild animals all over the world. Various fluorinated alternatives such as GenX have been developed to replace the long-chain PFASs, but there is limited information about their potential toxicity.

Methods:The current study developed blood culture protocols to assess the response to toxic compounds in the marsupial, Monodelphis domestica. After whole-blood culture conditions were tested and optimized, changes in gene expression in response to PFOA and GenX treatment …

Minutes 2-14-2023 | Safety Committee, Megan Meade

Safety Committee Minutes

No abstract provided.

Spartan Daily, February 14, 2023, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2023

Volume 160, Issue 8

The Indiana Statesman, February 14, 2023, Indiana State University

Student Newspaper

MSP invites to Inspiring Women Series; SoM hosts president’s concert; Pauli Murray to appear on U.S. currency