Digital Commons Network^™

Heterochiral Dna Nanotechnology For Biomedical Applications, Tracy L. Mallette

Biomedical Engineering ETDs

In the past 30 years, there have been major advancements on how to treat and diagnose disease because of the improvement and increase in accessibility of sequencing technology. Nucleic acid-based therapeutics can manipulate protein expression. Likewise, pathogens can be identified and detected with single nucleotide specificity. However, the underlying oligonucleotide technology requires protection against natural defense systems that have evolved to destroy foreign nucleic acids. Many chemical modifications that can protect nucleotides also have significant cytotoxic side effects and must be carefully designed into the strands. A novel way to protect against nuclease-mediated degradation is through the use of mirror-image, …

High Altitude Exercise, Autophagy, And Angiogenesis, Quint Nikolas Berkemeier

Health, Exercise, and Sports Sciences ETDs

Environmental hypoxia, as a result of decreased barometric pressure upon ascent to high altitudes (>2500m) presents increased physiological demands compared to low altitudes. Competitive athletes, mountaineers, wildland firefighters, military personnel, miners, and outdoor enthusiasts commonly participate in, or are exposed to, forms of exercise or physical labor at moderate to high altitudes. Interestingly, however, the majority of research on intermittent hypoxic exposure is centered around hematological markers and the skeletal muscle cellular responses to exercise in hypoxic environments remain largely unknown. Two processes that are integral for maintenance of cellular health in skeletal muscle include autophagy and angiogenesis. We …

Myeloid Fth1 Deficiency Protects Mice From Colitis And Colitis-Associated Colorectal Cancer Via Reducing Dmt1-Imported Iron And Stat3 Activation, Zhaoli Liu, Mariella Arcos, David R. Martin, Xiang Xue

Pathology Research and Scholarship

BACKGROUND: Myeloid cells are critical for iron and immune homeostasis. Ferritin heavy chain (FTH1) is essential for intracellular iron storage. Myeloid FTH1 is important in the pathogenesis of many inflammatory diseases. However, the role of myeloid FTH1 in colitis and colitis-associated cancer has not been determined.

METHODS: Myeloid FTH1 deficient and wild-type mice were treated with dextran sodium sulfate (DSS) or azoxymethane (AOM)-DSS to compare their susceptibility to acute colitis or colitis-associated cancer.

RESULTS: Myeloid FTH1-deficient mice fed with a high-iron diet were less susceptible to DSS-induced acute colitis than wild type mice. Mechanistic studies showed that myeloid FTH1 deficiency …

An Investigation Of Freshwater Turtle Ecology Using Stable Isotopes, Jonathan Duran

Biology ETDs

In 2022 I investigated niche partitioning among native and invasive freshwater turtles across three sites in the Middle Rio Grande using bulk stable isotope analysis. I found high degrees of niche partitioning at sites in which Red-eared sliders (Trachemys scripta) have yet to establish. However, at sites in which red-eared sliders are now the most abundant species they overlapped greatly in isotopic niche with all native turtles, in particular the painted turtle (Chrysemys picta). Using these same techniques, I then examined the trophic interactions between red-eared sliders and their acanthocephalan parasites. My results suggest that acanthocephalans …

Thermoregulation In Nocturnal Volant Endotherms Occupying Hot Deserts, William A. Talbot

Biology ETDs

The study of thermoregulation is of growing concern in this era of rapid climate change. Earlier studies such as those pioneered by Scholander and Bartholomew directed focus toward the study of endotherm survival in conditions of cold weather adversity. As techniques evolved in the measurement of parameters quantifying thermoregulation, metabolism and energy allocation, the standardization procedures has increased the comparability of data from diverse taxa. We can now search historical records and current research for explanations to the changes in the distribution, migration, extirpation or survival of animal and plant populations through time. It is increasingly common to observe dramatic …

Resolving The Paradox Of Polyploidy: Underexplored Facets Of Polyploid Plants, Benjamin P. Gerstner

Biology ETDs

Polyploidy, or whole genome duplication, is a common phenomenon in plants, but the establishment and persistence of mixed-ploidy populations remains a paradox. This dissertation explores factors that contribute to the persistence and establishment of mixed-ploidy populations in nature. The first chapter investigates the role of unreduced gametes in neopolyploid establishment and finds that variability in their formation rate can have a significant impact on polyploid establishment and persistence. The second chapter searches for evidence of soil microbes exhibiting ploidy-specificity, a pre-condition for microbe-mediated niche differentiation, a possible stabilizing mechanism contributing to ploidy coexistence. Finally, the third chapter tests for microbe-mediated …

Synthetic Biology Enabled Modification Of Bone Cells And Impact On Bone Mineral Resorption, Dulce C. Hayes

Biomedical Sciences ETDs

Development of a cell culture based bone model can contribute to biomedical research by producing a reproducible model system that mimics a bone remodeling disorder and reduces the use of animals in research studies. Synthetic biology was used to engineer osteoblast cell lines to alter a key protein in the RANKL pathway in efforts to control the bone remodeling process. Osteoblast cell lines, K7M2 and MC3T3-E1 Subclone 4 were engineered to express osteoprotegerin (OPG) and inhibit OPG expression, respectively. The upregulation of OPG expression was confirmed with Real Time PCR and Quantikine® Immunoassay. To identify the functionality of the engineered …

Generative Artificial Intelligence And Metaverse: Future Of Work, Future Of Society, And Future Of Humanity, Yuxin Liu, Keng Siau

Research Collection School Of Computing and Information Systems

The rapid development of Generative Artificial Intelligence (GenAI) and the emergence of the Metaverse are dynamically reshaping our lives and societies. GenAI can enhance the development of Metaverse and empower the applications in Metaverse. Metaverse is also an excellent environment for GenAI to demonstrate its power and usefulness. This interwoven relationship fuels the potential of integrating GenAI and Metaverse. The paper discusses the integration potential of GenAI and Metaverse from four aspects. We further investigate how GenAI, Metaverse, and the integration of GenAI and Metaverse can reshape our future across the realms of work, society, and humanity. This paper offers …

Diffusion Variational Autoencoder For Tackling Stochasticity In Multi-Step Regression Stock Price Prediction, Kelvin J.L. Koa, Yunshan Ma, Ritchie Ng, Tat‑Seng Chua

Research Collection School Of Computing and Information Systems

Multi-step stock price prediction over a long-term horizon is crucial for forecasting its volatility, allowing financial institutions to price and hedge derivatives, and banks to quantify the risk in their trading books. Additionally, most financial regulators also require a liquidity horizon of several days for institutional investors to exit their risky assets, in order to not materially affect market prices. However, the task of multi-step stock price prediction is challenging, given the highly stochastic nature of stock data. Current solutions to tackle this problem are mostly designed for single-step, classification-based predictions, and are limited to low representation expressiveness. The problem …

A Computational Approach For The Discovery And Characterization Of Oligonucleotide Aptamers For Protein Targets, Godfred Sabbih

Masters Theses and Doctoral Dissertations

Aptamers are short polynucleotide chains that can bind with very high affinities to a broad range of chemical and biomolecular targets, including proteins, cells, viruses, microorganisms, toxins, and chemical compounds. They are analogous to antibodies with applications in therapeutic delivery, biosensors, and diagnostics, but possess the advantages of easy production and lower immunogenicity than antibodies. They are produced by the experimental method known as the systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a powerful experimental method for selecting oligonucleotide aptamers for chemical or biomolecular targets of interest. The SELEX protocol has seen several modifications since its development …

Developing A Predictive Geospatial Habitat Model For A Rare Species Of Salamander In Tennessee: A Case Study For The Green Salamander (Aneides Aeneus), Erin Gaylord

Masters Theses and Doctoral Dissertations

Green Salamanders (Aneides aeneus; Cope and Packard 1881) are a secretive and cryptic species of salamander that can be relatively difficult to detect, due to their occupation of arboreal habitats. The incorporation of geospatial tools is critical in developing models that can be used to predict undocumented locations in Tennessee and elsewhere. Locating the species in Tennessee was accomplished through a total of 18 localities and with that, several habitat characteristics were able to be identified including, but not limited to: appropriately shaded and structured rocks, typical fauna co-occurring within the area, and general topography of the area. Of the …

Influence Of Abiotic Drivers And Plant Community Interactions On Bald Cypress (Taxodium Distichum) Seedlings: Implications For Restoration, Victoria Ellis

Biological Sciences Theses & Dissertations

Forested freshwater wetlands are valuable ecosystems that provide habitat for numerous species, sequester carbon, and act as sinks for excess water and nutrients. Historically, these ecosystems have been heavily degraded by anthropogenic activities leading to loss of ecosystem services and a desire to restore freshwater forested wetland habitat. Thus, science-backed approaches for the restoration of freshwater forested wetlands are necessary to ensure restoration goals are met. This body of research employed the Stress Gradient Hypothesis to test whether a multi-species planting approach using Juncus effusus (L.) (soft rush) could facilitate the survival of Taxodium distichum (L.) Rich. (bald cypress) seedlings …

Resistance Narratives: Storytelling Of Transnational Insurgencies In 1960-70s Us And Mexico, Tania Libertad Balderas

English Language and Literature ETDs

Resistance Narratives: Storytelling of Transnational Insurgencies in 1960-70s US and Mexico emphasizes how the narratives from the Mexican Insurgency, the American Indian Movement (AIM), and the leftist faction of the Chicana/o Movement in the 1960s and 1970s articulate intersecting notions of resistance, liberation, and transnational solidarity. The comparative analysis of the testimonial novel Las mujeres del alba (2019) by Chihuahuan novelist Carlos Montemayor, the autobiographies Lakota Woman (1991) and Ohitika Woman (1993) by Sičháŋǧu Lakȟóta writer and AIM militant Mary Brave Bird (formerly Crow Dog), and the memoirs and plays by the San Diego-based group Teatro de las Chicanas, collected …

Witchy Politics: Witches And Witchcraft As Political Tropes From Malleus Malleficarum (1487) To Les Sorcières De La République (2016) And The Mercies (2020), Mallaury Joëlle Marie Gauthier

Foreign Languages & Literatures ETDs

The focus of this thesis are two recent novels featuring witches: Chloé Delaume’s Les Sorcières de la République(The Witches of the Republic, 2016) and Kiran Millwood Hargrave’s The Mercies (2020). The first is a futuristic dystopia set in 2062, during the witch trial of the Sibyl of Cumae. The second is a work of historical fiction based on witch trial records and set in seventeenth-century Finnmark (Norway). Both are feminist novels, and both emphasize the political valence of the witch as a gendered figure. This figure emerged from the misogyny of early modern demonology but acquired its contemporary contours …

Role Of Endophytic Fungi On Arsenic Uptake In Solution: Insights For Bioremediation, Taylor Lillian Busch

Civil Engineering ETDs

Hazardous metals mobilized from natural deposits and mining legacy are environmental and human health concerns. The waters of our partner Native American communities affected by uranium and gold mining legacy exceed the EPA MCL of 10 µg/L arsenic (As). Bioremediation is a novel technology that uses native resources to remediate metals in the soil and groundwater. Plant-fungi symbiosis for bioremediation is widely studied, but the role of endophytic fungi in metal uptake is not well understood. The objectives of this study are to 1) determine As uptake by symbiotic endophyte Cadophora sp. and little bluestem grass (Schizachyrium scoparium) …

Queer Crises: Movements From Queerness And Feelings Of White Religion In The United States, Austin Williams Miller

Communication ETDs

Anchored by contemporary crises surrounding queer and trans people in the United States, I employ movements from queerness within an affective queer phenomenological framework to understand how arrangements of “white religion” (Schaefer, 2015, p. 63), a process whereby U.S. American Christian forms escape ideology into religious affective economies in the United States, relegate queer people “to the background… to sustain a certain direction” (Ahmed, 2006, p. 31). I assemble a queer rhetorical context analyzing white religious space in documentary film, secular sexual regulation through contemporary U.S. legal contexts around marriage, and settler colonial Christian nationalist political imaginations to critique how …

Slavery And Architecture Across The Mediterranean, John Behnken

History ETDs

Enslaved people as architectural material, found in the cultural examples of the Great Mosque of Cordoba and the Hagia Sophia, provide a lens from which scholars can re-envision the historical narrative. The scholarship surrounding the development and transition of the Great Mosque of Cordoba from a mosque to a church, elicits new research into what medieval people thought about race, race-making, and cultural ownership. The conceptions of race are evident through the medieval paradigms of enslavement. Who could and could not become enslaved establish social, cultural, and phenotypic classifications which in turn become race. The work of scholars such as …

The Haunting Aesthetics Of Empire: Filipinx America, Us Empire, And Cultural Production, Alana J. Bock

American Studies ETDs

Throughout this dissertation, I argue that US imperial knowledge production affirms US exceptionalism by disavowing the imperial violence wrought on the Philippines and its people. This disavowal not only renders the Philippines and Filipinx bodies illegible, but also haunts the Filipinx American diaspora. I argue that the haunted logics of empire are a set of relations, rather than specters of specific times and places, in which knowledge and power work together to continually produce and reproduce a specific and limiting reality and sensorium through which to view the world. In my interrogation of empire’s haunted logics, I not only look …

Greening The Desert: Emirati Youth’S Perceptions Of Green Branding, Gergana Alzeer, Tilde Rosmer

All Works

This chapter focuses on Emirati youth’s understanding of and practices related to a green shift in the UAE and how this correlates with the state’s efforts to brand the UAE as green and sustainable. This is part of a larger research project that investigates Emirati youth’s understanding of climate change. The UAE experience of environmental sustainability is unique as its green shift was initiated by the government in a top-down approach compared to the bottom-up green movements in most western states that has been the focus of most environmental studies so far. Environmental sustainability is part of UAE’s national Agenda …

The Mindful First Amendment, Gary Myers

New Mexico Law Review

The mindfulness movement has begun to play an expanding role in personal well-being and in society more generally. Although there is an active push for mindfulness in law, its primary focus is on ways in which mindfulness techniques can help lawyers in their personal and professional lives. This article explores the possible contributions of mindfulness to the widely recognized challenges facing freedom of speech and freedom of the press in an era of severe cultural and political polarization. This article focuses on the external aspects of mindfulness—the interaction with others and with society—to assess whether its techniques might assist in …

Military Women In World Cinema: A 20th Century History And Filmography, Introduction, Deborah A. Deacon, Stacy Fowler

Faculty Articles

From British soldier Flora Sandes to the fame World War II Night Witches of the Soviet Air Force, women across the globe stepped up to defend their countries during every major and minor conflict of the twentieth century, and filmmakers have long attempted to capture their stories.

This book analyzes real and fictional military women's portrayals in world cinema, including movies from Israel, the United Kingdom, Italy, China, France, the Soviet Union, and others. It includes theatrical releases, direct-to-video productions, and made-for-television films.

Chapters, organized by decade, address topics including the women's sexuality, maternal and marital status, leadership skills, actual …

Salinity In Propagation: Germination And Juvenile Acclimatization Of Wetland Halophytes Using Saline Irrigation, Morgan Tomlin

All Theses

The introduction of poor water quality into commercial nursery crop production is predicated on optimized methods that apply saline irrigation without compromising plant success and health. Halophytes have many mechanisms of salt tolerance; however, these are greatly tied to physiological and developmental maturity. Thus, evaluating salt tolerance of halophytic crops (Hibiscus moscheutos and Kosteletzkya virginica) during seed germination and juvenile phases of life may provide insight into the efficacy of integrating poor quality water in horticultural operations.

Four seed priming methods (hydropriming, proline priming, low concentration halopriming, and high concentration halopriming) were evaluated as pre-sowing techniques to ameliorate …

Evaluating The Effect Of Biochar Soil Amendments On Belonolaimus Longicaudatus Populations Damaging Bermudagrass In South Carolina, Malone Thomason

All Theses

Belonolaimus longicaudatus Rau, (1958) (sting nematode) is a prevalent plant-parasitic nematode found in association with Cynodon spp. (bermudagrass) in South Carolina (Ye et al., 2012). Due to the persistence of the pest, long-term management strategies are necessary. Applications of biochar and compost amendments have reduced nematodes in various crop systems, but research on turfgrass systems is limited. The objectives of the studies were to determine: (1) The effect of topdressing or soil incorporated biochar amendments on sting nematode populations, and (2) The effect of topdressing or soil incorporated amendments on turf quality. A field study was established to examine topdressings …

Comparison Of Vegetative Community And Soil Organic Matter Depth Among Reference Sites And Two Restored Wetlands In The Coastal Plain Of South Carolina, Jessica Clark

All Theses

Wetland loss and degradation from agriculture, urbanization, forestry, and mining is a global issue. South Carolina alone has lost over 27% of its wetlands. This historical wetland loss and climatic changes and impacts make restoring wetlands critical for the state. In restoration, understanding the difference in restored and reference wetland’s vegetation and soil organic matter depth can be crucial in assessing the recovery rate and determining environmental functions and services. The main objectives for our research were to determine differences in soil organic matter depth and vegetative community between the restored Brosnan Forest wetlands, the headwater flats and headwater slopes, …

Examining Biological Algal Harvest For Use In Clemson University’S Algal Carbon Capture System Using Freshwater Mussel Elliptio Complanata, Jack Grimes

All Theses

Capture of atmospheric carbon by freshwater algae is being proposed in Clemson University’s outdoor algal carbon capture system, formerly described as the Partitioned Aquaculture System (Drapcho and Brune, 2000). Several algal species have been present in the system, depending on cultivation conditions. However, mechanical harvesting of the algae has proven difficult because of the cost of chemical flocculants, the energy cost of filtration due to small particle size of algae, and the relatively dilute algal cell concentrations in the system. Prior research on algal filtration by freshwater mussels has indicated its potential for use as a biological filter. This research …

Patterns And Drivers Of Wiregrass Gap Longleaf Pine (Pinus Palustris Mill.) Woodland Succession As Part Of Restoration Efforts, Armin Weise

All Theses

Longleaf Pine (Pinus palustris) communities are widespread throughout the Southeastern United States with a dominant understory vegetation of wiregrass (Aristida spp.) in most of its range. A small area in central South Carolina that is naturally free of wiregrass is called the “Wiregrass Gap”. Here, the understory vegetation is dominated by bluestems grasses (Andropogon spp. and Schizachyrium spp.) which drive the disturbance regime of frequent low-intensity fire. The successful establishment of these grasses is key for longleaf pine woodland restoration efforts in this region, but few resources detail the ecological drivers at play that enable successful restoration in these longleaf …

Measurement Of Microplastics In A Freshwater Micro-Watershed, Cole Bowman

All Theses

The role of microplastics as an emerging contaminant can be complex to study because of the lack of standardization in the collection and analysis of microplastic particles. The purpose of this manuscript is to detail an iterative process of different collection methods in trying to understand microplastic deposition and transport in a freshwater micro-watershed through lab-based and field-based studies. A flume experiment was conducted to test the trapping efficiency of a sediment trap design at two different velocities (1.12 m/s and 2.24 m/s). Eight types of microplastics varying in morphology and density were introduced to the flume in order to …

Consistency Is Key: Investigating Vocal Consistency In Field Sparrows, Stephanie Stanton

Masters Theses

No abstract provided.

Cerebellar Dysfunction As A Source Of Dystonic Phenotypes In Mice, Amanda M Brown, Meike E Van Der Heijden, H A Jinnah, Roy V Sillitoe

Duncan NRI Faculty and Staff Publications

There is now a substantial amount of compelling evidence demonstrating that the cerebellum may be a central locus in dystonia pathogenesis. Studies using spontaneous genetic mutations in rats and mice, engineered genetic alleles in mice, shRNA knockdown in mice, and conditional genetic silencing of fast neurotransmission in mice have all uncovered a common set of behavioral and electrophysiological defects that point to cerebellar cortical and cerebellar nuclei dysfunction as a source of dystonic phenotypes. Here, we revisit the Ptf1aCre/+;Vglut2flox/flox mutant mouse to define fundamental phenotypes and measures that are valuable for testing the cellular, circuit, and …

Ancestral Diversity In Lipoprotein(A) Studies Helps Address Evidence Gaps, Moa P Lee, Sofia F Dimos, Laura M Raffield, Zhe Wang, Anna F Ballou, Carolina G Downie, Christopher H Arehart, Adolfo Correa, Paul S De Vries, Zhaohui Du, Christopher R Gignoux, Penny Gordon-Larsen, Xiuqing Guo, Jeffrey Haessler, Annie Green Howard, Yao Hu, Helina Kassahun, Shia T Kent, J Antonio G Lopez, Keri L Monda, Kari E North, Ulrike Peters, Michael H Preuss, Stephen S Rich, Shannon L Rhodes, Jie Yao, Rina Yarosh, Michael Y Tsai, Jerome I Rotter, Charles L Kooperberg, Ruth J F Loos, Christie Ballantyne, Christy L Avery, Mariaelisa Graff

Faculty, Staff and Student Publications

INTRODUCTION: The independent and causal cardiovascular disease risk factor lipoprotein(a) (Lp(a)) is elevated in >1.5 billion individuals worldwide, but studies have prioritised European populations.

METHODS: Here, we examined how ancestrally diverse studies could clarify Lp(a)'s genetic architecture, inform efforts examining application of Lp(a) polygenic risk scores (PRS), enable causal inference and identify unexpected Lp(a) phenotypic effects using data from African (n=25 208), East Asian (n=2895), European (n=362 558), South Asian (n=8192) and Hispanic/Latino (n=8946) populations.

RESULTS: Fourteen genome-wide significant loci with numerous population specific signals of large effect were identified that enabled construction of Lp(a) PRS of moderate (R

CONCLUSIONS: …