Digital Commons Network^™

Use Of Comparative Genomics And In Vitro Screening Approach To Identify Vaccine Candidates For The Food-Borne Pathogen Campylobacter Jejuni, Sabin Poudel

Theses and Dissertations

Campylobacteriosis is a leading foodborne illness worldwide, primarily caused by Campylobacter jejuni (C. jejuni) which is associated with poultry consumption. The emergence of antibiotic resistance has emphasized the need for alternative strategies to control C. jejuni colonization in poultry. To assess the prevalence of C. jejuni in poultry, 270 cloacal swab samples were collected from broilers raised under No-Antibiotics Ever system. Among these samples, 16.3% were identified as C. jejuni positive. Notably, these isolates exhibited a diverse range of virulence factors and antimicrobial resistance genes, with 61.36% of isolates showing hyper-motile and 20.45% demonstrating multidrug resistance. Following isolation, whole genome …

An Analysis Of Coastal Restoration Projects In Alabama And Mississippi, Barbara Nyarkoa Okai

Theses and Dissertations

This study aims to review thirteen coastal restoration projects considering the various ecosystem services provided by restoration and estimates the economic value of one of the ecosystem services of restoration. These ecosystem services include water quality improvement, fish and benthic species productivity, shoreline stabilization, oyster abundance, and marsh growth. The projects represent a set of large-scale projects within Alabama and Mississippi, with construction and monitoring costs ranging from $2.3 million to $50 million per project. To determine the economic value of one of the ecosystem services of coastal restoration projects, I used the meta-analysis method to estimate the willingness to …

The Response Of Bats And Their Insect Prey To Different Coastal Upland Habitat Management Techniques, Amanda Nicole Sartain

Theses and Dissertations

Declining bat populations necessitates a need to understand how different land management techniques influence bat activity. This study assessed the influences of different coastal upland habitat management techniques, such as mulching, prescribed fire, and select cut, on forest bat activity within the Grand Bay National Wildlife Refuge and National Estuarine Research Reserve. Acoustic recorders were used to monitor bat activity and insect and vegetation surveys were used to assess influences on bat activity across different land management techniques. Results demonstrate that overall bat activity was similar across different land management techniques, however larger species adapted for open-space flying were shown …

Is A Test The Best?, Giulia Ronnette Mcdonald

Theses and Dissertations

The test effect (Roediger & Karpicke, 2006) demonstrates that performance on a final test is better when an immediate test is taken after reading a passage rather than rereading. Although transfer appropriate processing has been suggested as a theoretical explanation, the results could be due to elaborative retrieval processes during the immediate test that are not available during restudy. We compared testing to three other strategies using elaborative retrieval—Generating Questions, Read-Recite-Review, Teaching—to determine whether they would be as beneficial as testing. Results showed that each of the alternative strategies produced final test performance equal to that of testing, suggesting not …

Loaded Lipid Emulsified Volatile Anesthetics In Canine Primary Hepatocytes, Patricia De Carvalho Ibrahim Obeid

Theses and Dissertations

In the 19th century, halothane hepatitis became a sensitive and well-known subject in human anesthesiology due to the production of a noxious metabolite further discovered, trifluoroacetic acid. Subsequently, isoflurane, enflurane, and desflurane were also investigated for potentially causing hepatitis through the same metabolite. Sevoflurane, however, does not generate trifluoroacetic acid and is quickly conjugated and excreted.

For more than four decades these anesthetics have been experimentally developed for intravenous injection by having added either a lipid or fluorocarbon-based carrier to produce general anesthesia with less drug and faster onset of action. The use of intravenous emulsified halogenated anesthetics as an …

Multi-Objective Design Optimization Of Two Configurations Of Ventricular Shunts For Hydrocephalus, Will Kirkpatrick

Theses and Dissertations

Hydrocephalus is developed when the flow of cerebrospinal fluid is obstructed in the ventricles and a pressure build-up is generated within the brain. Ventricular shunts are used to remove excess fluid from the brain, but these shunts have a common problem of failure due to the shunt being obstructed by the build-up of astrocytes. To address this, two sets of 27 designs of ventricular shunts were identified and analyzed with parameters that could potentially reduce obstruction risks. The performance of these designs was examined using fluid simulations on these two sets of 27 designs. One set explored close-tipped shunt designs, …

Comparison Of Aging Techniques In Wild Bottlenose Dolphins (Tursiops Truncatus) Of Unknown Age From The Mississippi Sound., Christa Elizabeth Barrett

Theses and Dissertations

Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding their health. There are many methods of aging dolphins including morphometrics, counting growth layer groups (GLGs) of teeth, comparing dentin to pulp cavity ratio on dental radiographs, analysis of the pectoral flipper radiographs, and epigenetic testing. The most common method for aging toothed cetaceans is the counting of GLGs in the teeth. Since there are many challenges associated with aging dolphins based on the counting of GLGs, a processing technique that results in better resolution of GLGs is needed. This is a two-part study that …

Effect Of Environmental And Geometrical Factors On Microstructure, Desiccation Cracking, And Carbon Dioxide Flux In Clays, Charles Clayton Goodman

Theses and Dissertations

Studying the effects of extreme conditions, such as high temperatures and low humidity, on soil properties is important to various disciplines, including geotechnical engineering, soil science, waste management, crop management, and ceramics. The goal of this research is to investigate the effect of environmental and geometrical factors on microstructure, desiccation cracking, and CO₂ flux in clays. The objectives of this research are threefold. (1) Understand the effects of temperature on the microstructure of clay soils; (2) develop a standardized procedure for studying desiccation cracking in a laboratory setting with reliable and repeatable results; and (3) develop an environmental chamber …

A Survey Analysis Of Southeastern U.S. Dairy Producers’ Emotional States And Their Subjective Pain Perception Of Dairy Cows, Michelle M. Schuh

Theses and Dissertations

Dairy producers play a central role in evaluating and seeking treatment or care for animal pain. The primary aim of this study is to examine dairy producers’ emotional states and professional quality of life and analyze the relationship between these variables and their perception of pain in dairy cattle. Dairy farm owners and managers of 65 southeastern U.S. herds participated in a survey that included demographic information, the Depression, Anxiety, and Stress Scale, a modified Professional Quality of Life Scale, and 23 items requiring participants to evaluate various painful conditions in cattle.

Object Itineraries Of Metal Artifacts From The Stark Farm Site Complex (22ok778), Madeleine Marie Hale

Theses and Dissertations

This thesis focuses on creating a deeper understanding of European-made metal objects uncovered at a Late Mississippian period site by using an object itinerary theoretical framework. This theory allows for objects to be understood and analyzed without bias as it acknowledges Indigenous and archaeological perspectives by considering the many different contexts an object moves through. I apply this theory to these European-made metal objects that were transformed and used by the Chicasa as a way to introduce a more collaborative and holistic approach to the other analytical methods being used at Stark Farm (22OK778). This process was completed by using …

Upland Cotton And Nematodes: An Analysis Of Historical Resistance, Upcoming Threats, And Co-Inoculation Effects, Amanda Gaudin

Theses and Dissertations

Upland cotton (Gossypium hirsutum ) is an important fiber crop grown throughout the southern United States. Plant-pathogenic nematodes are worm-like animals that feed on the roots of most agronomic crops, including cotton. The southern root-knot nematode (Meloidogyne incognita, RKN) and the reniform nematode (Rotylenchulus reniformis, RN) cause significant yield losses in cotton every year. Current sources of resistance are effective but limited, therefore historical screenings of cotton accessions were revisited in search for novel resistance sources. None were identified but many of the screened accessions possessed markers of known root-knot nematode and reniform nematode resistance. …

Non-Destructive Evaluation Of White Striping And Microbial Spoilage Of Broiler Breast Meat Using Structured-Illumination Reflectance Imaging, Ebenezer O. Olaniyi

Theses and Dissertations

Manual inspection is a prevailing practice for quality assessment of poultry meat, but it is labor-intensive, tedious, and subjective. This thesis aims to assess the efficacy of an emerging structured illumination reflectance imaging (SIRI) technique with machine learning approaches for assessing WS and microbial spoilage in broiler breast meat. Broiler breast meat samples were imaged by an in house-assembled SIRI platform under sinusoidal illumination. In first experiment, handcrafted texture features were extracted from direct component (DC, corresponding to conventional uniform illumination) and amplitude component (AC, unique to the use of sinusoidal illumination) images retrieved from raw SIRI pattern images build …

Evaluating The Role Of The Bovine Vaginal Microbiome In Neonatal And Maternal Health Outcomes, Riley D. Messman

Theses and Dissertations

The dam vaginal microbiota is the first major microbial inoculating community within the neonate. The composition of the dam vaginal microbiota has implications in calf commensal microbiota development. Alterations of the dam microbial community prior to parturition could alter inoculating communities and immune responses in both the dam and calf. Thus, authors aimed to elucidate the microbial community composition of the bovine dam vaginal and calf nasal microbiota post-partum after utilizing betadine lavages (BL). The dam vaginal and calf nasal microbial communities and immune responses were evaluated at 0-, 15-, 30- and 60-day post-partum. Microbiota composition of the dam haircoat, …

Determining The Best Indicators For Targeted Selective Treatment Development Against Gastrointestinal Nematodes In Sheep And Goats In Mississippi, Lindsey N. Dearborn

Theses and Dissertations

An understanding of characteristics of sheep and goat producers across the Southeast United States is crucial for the development and vitality of the small ruminant industry. The state of Mississippi (MS) is rich in animal agriculture, and sheep and goats display the potential to become an integral part of the state’s agricultural livelihood. However, sheep and goat producers are constantly combatting the negative effects gastrointestinal nematodes inflict upon their animals. With the anthelmintic resistance status rising across the globe, sustainable methods to control parasites in small ruminants are crucial. Therefore, small ruminant producers must develop an accurate targeted selective treatment …

Oocytes To Offspring: Optimizing Assisted Reproductive Technologies (Art) To Support Amphibian Conservation, Shaina Shaira Lampert

Theses and Dissertations

The increasing dependence on amphibian captive breeding programs to support at-risk populations has led to growth in the application of assisted reproductive technologies (ART) to aid with the management of decreasing population density and genetic diversity. Already the integration of ART within these programs has led to monumental successes and shows great promise as a means to augment amphibian breeding efforts when natural breeding has low success. The focus of this project was to continue the optimization of ART to aid in making these technologies more widely applicable. Specific objectives include developing a framework that can be used to standardize …

Trace Metal Analysis Of Pond Sediments To Measure Deposition From A Coal-Fired Power Plant In Ackerman Mississippi, Charles Bills

Theses and Dissertations

Trace element sediment pollution due to coal power plants have been reported in many countries. In this study, pond sediment samples collected from locations around a coal fired power plant in Ackerman, MS, were evaluated for their trace element concentration and pollution status. The samples were collected at two depths to study changes in element accumulation over time. The samples were digested and the elements, As, Cd, Cr, Cu, Pb, Hg, and Zn were analyzed using ICP-MS. Results were then compared to known background concentrations as well as sediment quality guidelines and pollution thresholds. The sample locations downwind of the …

Timing Is Everything: Impacts Of Firing Technique And Season On Plant Communities In The Southeastern United States, Luke Michael Resop

Theses and Dissertations

Prescribed fire is a common land management tool in the southeastern United States. Historical fires occurred primarily in the growing season, but modern day prescribed fire is commonly restricted to the dormant season. Plant community responses to various fire regimes are relatively unknown, and managers require information on how fire regimes impact plant communities. To address these limitations, I studied the impacts of March and June fire on plant communities. Results indicated various woody midstory species respond differently to fire season and community response is driven by species composition. In another study, I examined impacts of February, May-June, and September-October …

Investigating Potential Indicators Of Soil Health Through Microbiome Response To Environmental And Anthropogenic Stressors, Austin Cook

Theses and Dissertations

Traditionally, the analysis of soil health has overlooked the biological component of soil due to poor understanding of connections between the microbiome and empirically measured soil health indicators. The purpose of this study was to assess the effects of environmental and anthropogenic stressors on the soil microbiome, with the aim of identifying measurable soil biological indicators. Chosen soils were examined under distinct conditions to evaluate the effect of selected environmental and anthropogenic stressors on the microbiome. Soil biological responses were analyzed via enzymatic response, microbial functional genes, and microbial community. Environmental factors such as soil moisture and organic matter showed …

Screening And Selection For Resistance To Gray Leaf Spot (Pyricularia Oryzae) In Early Planted Annual Ryegrass, Calyn M. Adams

Theses and Dissertations

Cattlemen in the southeastern United States widely plant annual ryegrass [Lolium perenne L. spp. multiflorum (Lam.) Husnot] (ARG). It is often planted earlier than the recommended window. This exposes juvenile stands to increased pressure from gray leaf spot (Pyricularia oryzae Cavara) (GLS), causing stand loss. This study was designed to screen for resistance to P. oryzae in two ARG genotypes, Vertyl and a heat-tolerant germplasm (HTARG5) with the objective of developing a crossbred population to be improved using restricted, recurrent phenotypic selection. Experiments were conducted from 2021 – 2022. Disease severity (DS) was assessed 3wk after inoculation with …

Dog Tired: Examining The Relation Between Dog And/Or Cat Ownership And Owners’ Sleep, Courtney J. Bolstad

Theses and Dissertations

Sleep is an essential part of life, and obtaining quality sleep is important for various areas of functioning. Behaviors to promote sleep include physical activity, managing stress, reducing anxiety symptoms, maintaining a consistent sleep schedule, and obtaining bright light exposure. Many of these behaviors overlap with benefits observed from pet ownership (PO). The present study aimed to examine the relation between PO and sleep as well as moderators and mediators of this relation. Four research questions were examined: 1) Is PO significantly related to sleep? 2) Does gender moderate the relation between PO and sleep? 3) Do sleep-promoting behaviors (i.e., …

Physical Layer Security With Unmanned Aerial Vehicles For Advanced Wireless Networks, Aly Sabri Abdalla

Theses and Dissertations

Unmanned aerial vehicles (UAVs) are emerging as enablers for supporting many applications and services, such as precision agriculture, search and rescue, temporary network deployment, coverage extension, and security. UAVs are being considered for integration into emerging wireless networks as aerial users, aerial relays (ARs), or aerial base stations (ABSs). This dissertation proposes employing UAVs to contribute to physical layer techniques that enhance the security performance of advanced wireless networks and services in terms of availability, resilience, and confidentiality. The focus is on securing terrestrial cellular communications against eavesdropping with a cellular-connected UAV that is dispatched as an AR or ABS. …

Integrating Weed-Suppressive Cotton Chromosome Substitution Lines And Cover Crops For Sustainable Weed Management In Cotton Production Systems., Alyssa Lea Miller

Theses and Dissertations

Weeds pose a challenge to cotton production, and herbicides have been the primary means of control. However, there is growing concern over weed resistance to herbicides. To address this issue, we evaluated three cover crop species and six cotton accessions for weed suppression. The study found that hairy vetch, wheat, and B16 cotton CS line effectively suppressed Amaranthus species, while MNTN 4-15, B16, hairy vetch, and wheat were correlated with the highest cotton yield. Cover crops were also analyzed for chlorogenic acid content, with wheat, MNTN 4-15, and hairy vetch producing the highest amounts. The greenhouse tray study showed that …

Identifying Additional Neuroprotective Mechanisms Of Novel Phenoxyalkyl Pyridinium Oximes Against Organophosphorus Compound Toxicity, Chiquita Yvette Price

Theses and Dissertations

Our laboratory has invented a series of oxime acetylcholinesterase (AChE) reactivators (US Patent 9,227,937) that enter the brain, reduce time to cessation of seizure-like activities, and prevent organophosphorus compound (OP) neuropathology, not seen with the current U.S. approved AChE reactivator, pralidoxime (2-PAM). Thus, 2-PAM fails to protect the brain against damage and long-term cognitive and behavioral deficits seen in humans after OP exposure. However, the mechanisms by which these novel oximes provide central neuroprotection through preservation of neuronal cell structures from damage in a rat model are not fully understood by AChE reactivation alone. This dissertation investigated neurotoxic mechanisms of …

Impact Of Soil Moisture Stress At Different Phases Of Corn Growth And Development, Ranadheer Reddy Vennam

Theses and Dissertations

Suboptimal soil moisture during the growing season often limits growth and yield potential of corn. This study aimed to assess the impact of varying soil moisture regimes on corn growth at different growth phases involving vegetative, flowering, and grain-filling stages. Exposure to moisture stress (80% of the control) during the vegetative stage resulted in a 65% reduction in stomatal conductance and increased the canopy temperature by 5 ^oC, which led to a substantial decrease in total dry matter (56%). Moisture stress-induced reductions in silk length (19%) and fresh weight (34%), negatively affected kernel number (53%), and weight (54%). Unlike …

The Effect Of An Aiming Device On The Accuracy Of Humeral Transcondylar Screw Placement, Katherine Neal

Theses and Dissertations

Canine cadaveric forelimbs (136) were randomly assigned to either treatment (aiming device) or control (fluoroscopic-guided) groups, and to either diplomate or resident for humeral transcondylar screw placement. Each limb underwent a transcondylar humeral screw placement from a lateral to medial direction. Digital radiography was used to evaluate screw trajectory, measured as deviation from the epicondylar line, and eccentricity on the humeral condyle. Procedure time, difference in outcome based on experience level, and complications were recorded. In the right limb, screw trajectory with the aiming device was decreased compared to fluoroscopy. There was no difference of accuracy between leg laterality with …

The Effect Of Water Flow Rates On The Survival And Growth Rates Of Three Caribbean Bouldering Coral Species Juveniles In An Indoor Versus Outdoor Environment, Ian Michael Johnson

All HCAS Student Capstones, Theses, and Dissertations

Coral reefs are vital ecosystems for the world’s oceans and humanity; however, they are threatened by climate change, disease, and local anthropogenic stressors, and need assistance to recover. Traditional reef restoration efforts (fragmentation and outplanting are helping but are limited in effectiveness by not increasing genetic diversity. Ex situ sexual propagation for corals provides new, genetically different coral recruits. However, this process is laborious, expensive and time consuming, especially at the scale required to effectively contribute to the widespread recovery. To lower costs, two key parameters that require optimization to hasten the growth of coral recruits are water flow and …

The Role Of Phenylacetic Acid (Paa) Catabolism In Acinetobacter Physiology, Anna Josephine Hooppaw

Arts & Sciences Graduate Student Theses and Dissertations

Microorganisms are constantly challenged by stressful environments and have evolved mechanisms to adapt to or counteract these threats. The Gram-negative pathogen Acinetobacter baumannii is thought to rely on a “persist and resist” strategy that facilitates its remarkable ability to survive in a variety of harsh conditions, giving it the capacity to cause life-threatening infections. Currently, over 60% of the global A. baumannii clinical isolates are multidrug resistant (MDR), leading the World Health Organization and the Centers for Disease Control and Prevention to categorize this pathogen as a top priority for the research and development of new antimicrobial therapies. Understanding the …

Distinct Roles For Ltα3 And Ltα1Β2 Produced By B Cells Contribute To Their Multi-Faceted Impact On Ileitis, Emma Claire Erlich

Arts & Sciences Graduate Student Theses and Dissertations

Crohn’s disease, one of the most common forms of inflammatory bowel disease, is marked by local intestinal inflammation. However, pathology in CD patients is not strictly limited to the gastrointestinal system, and almost 50% of CD patients exhibit extraintestinal manifestations of disease. Many, though not all, of these systemic pathologies are linked to intestinal inflammation and, thus, resolution of local gastrointestinal disease also ameliorates systemic disease. How the connection between local and systemic inflammation is mediated is not well understand, nor is why only some patients exhibit these extraintestinal manifestations of disease. Blocking dissemination of inflammatory signals generated in the …

Molecular Mechanisms Of Snorna-Ll-15 Crosstalk In Adipocyte Lipolysis And Nk Cell Rejuvenation, Yaohua Zhang, Zilong Zhao, Lisa A Huang, Yuan Liu, Jun Yao, Chengcao Sun, Yajuan Li, Zhao Zhang, Youqiong Ye, Fei Yuan, Tina K Nguyen, Nikhil Reddy Garlapati, Andrew Wu, Sergey D Egranov, Abigail S Caudle, Aysegul A Sahin, Bora Lim, Laura Beretta, George A Calin, Dihua Yu, Mien-Chie Hung, Michael A Curran, Katayoun Rezvani, Boyi Gan, Zhi Tan, Leng Han, Chunru Lin, Liuqing Yang

Faculty, Staff and Student Publications

Obesity, in which the functional importance of small nucleolar RNAs (snoRNAs) remains elusive, correlates with risk for many cancer types. Here, we identify that the serum copies of adipocyte-expressed SNORD46 correlate with body mass index (BMI), and serum SNORD46 antagonizes interleukin-15 (IL-15) signaling. Mechanically, SNORD46 binds IL-15 via G11, and G11A (a mutation that significantly enhances binding affinity) knockin drives obesity in mice. Functionally, SNORD46 blocks IL-15-induced, FER kinase-dependent phosphorylation of platelet glycoprotein 4 (CD36) and monoglyceride lipase (MGLL) in adipocytes, leading to inhibited lipolysis and browning. In natural killer (NK) cells, SNORD46 suppresses the IL-15-dependent autophagy, leading to reduced …

Modeling Subcellular Specificity In The Developing Retina, Ross Perez, Yong Park, Arlene Hirano, Nicholas Brecha, Benjamin Frankfort, Elizabeth Zuniga-Sanchez

Faculty, Staff and Students Publications

The precise wiring of the nervous system relies on neurons extending their processes at the right time and place to find their appropriate synaptic partner. The mechanisms that determine when and where neurons extend their neurites during synaptogenesis remains a central question in the field. In the present study, we developed a cell culture system coupled with live imaging to investigate the wiring mechanisms in the developing nervous system. We focused on horizontal cells which are interneurons in the mammalian outer retina known to synapse selectively to distinct photoreceptors. Our data shows cultured horizontal cells extend neurites in a similar …