Digital Commons Network^™

Pyruvate Anaplerosis Is A Targetable Vulnerability In Persistent Leukaemic Stem Cells, Kevin M Rattigan, Zuzana Brabcova, Daniele Sarnello, Martha M Zarou, Kiron Roy, Lucie De Beauchamp, Angela Ianniciello, Ahmed Khalaf, Eric R Kalkman, Mary T Scott, Karen Dunn, David Sumpton, Alison M Michie, Mhairi Copland, Saverio Tardito, Eyal Gottlieb, G Vignir Helgason

Faculty, Staff and Student Publications

Deregulated oxidative metabolism is a hallmark of leukaemia. While tyrosine kinase inhibitors (TKIs) such as imatinib have increased survival of chronic myeloid leukaemia (CML) patients, they fail to eradicate disease-initiating leukemic stem cells (LSCs). Whether TKI-treated CML LSCs remain metabolically deregulated is unknown. Using clinically and physiologically relevant assays, we generate multi-omics datasets that offer unique insight into metabolic adaptation and nutrient fate in patient-derived CML LSCs. We demonstrate that LSCs have increased pyruvate anaplerosis, mediated by increased mitochondrial pyruvate carrier 1/2 (MPC1/2) levels and pyruvate carboxylase (PC) activity, in comparison to normal counterparts. While imatinib reverses BCR::ABL1-mediated LSC metabolic …

Defining The Roles Of Pyruvate Oxidation, Tca Cycle, And Mannitol Metabolism In Methicillin-Resistant Staphylococcus Aureus Catheter-Associated Urinary Tract Infection, Santosh Paudel, Sarah Guedry, Chloe L P Obernuefemann, Scott J Hultgren, Jennifer N Walker

Faculty, Staff and Student Publications

Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of complicated urinary tract infection (UTI) associated with the use of indwelling urinary catheters. Previous reports have revealed host and pathogen effectors critical for MRSA uropathogenesis. Here, we sought to determine the significance of specific metabolic pathways during MRSA UTI. First, we identified four mutants from the Nebraska transposon mutant library in the MRSA JE2 background that grew normally in rich medium but displayed significantly reduced growth in pooled human urine (HU). This prompted us to transduce the uropathogenic MRSA 1369 strain with the transposon mutants in sucD and fumC (tricarboxylic acid …

Variability Of Total Mercury Concentrations In Steller Sea Lion Bone Locations And Bone Elements, Mary P. Keenan

Marine Biology

Mercury (Hg) is a contaminant of global concern with impacts on the health and resilience of marine wildlife. Some forms of ingested Hg are bioaccumulated and biomagnified with greatest concentrations observed in long-lived top predators. High concentrations of Hg in mammals impair immune function, neurochemistry, and reproduction. Steller sea lions (Eumetopias jubatus, SSL) are a long-lived top predator, and some populations have total mercury concentrations ([THg]) in fur that are above the threshold values of concern for toxic effects. While this information is available for present-day populations of SSLs, [THg] in historic and ancient populations of SSLs are unknown. Bone …

Full Issue

Insights: The Newsletter of the Neal A. Maxwell Institute for Religious Scholarship

No abstract provided.

On The Right Or Left: Benjamin And The Scapegoat, Terrence L. Szink, John W. Welch

Insights: The Newsletter of the Neal A. Maxwell Institute for Religious Scholarship

Over the years, scholars have found in King Benjamin's speech much that is at home in the ancient Israelite autumn festivals surrounding the Day of Atonement. An essential part of that day was the scapegoat ritual.

Review Of Leon-Boys, Diana. Elena, Princesa Of The Periphery: Disney’S Flexible Latina Girl. Rutgers University Press. 2023. 182 Pp. $26.95 Paperback. Isbn# 978-1978830172, Bryce Henson

Feminist Pedagogy

No abstract provided.

The Feminist Keyword Project: Literature Reviews As Feminist Praxis, Krystal Cleary

Feminist Pedagogy

The Feminist Keyword Project is a scaffolded series of assignments that culminates in a literature review of the student’s chosen keyword. It is designed for intermediate undergraduate students who acquired a foundational feminist vocabulary in their introductory classes. Building upon this baseline fluency, the project shifts students’ understanding of course concepts from a glossary of terms to politicized feminist gatherings. The project is informed by the tradition of the keyword entry genre to move students beyond merely defining their chosen term to narrating it as a site of feminist discourse in and outside of academia. In doing so, the Feminist …

A Framework For Creating Virtual Reality Models For More Effective Coastal Flood Risk Communication, Tina Korani, Alexandrea Martinez

CSU Journal of Sustainability and Climate Change

Coastal cities are exposed to increasing risks of flooding from sea-level rise. Climate change is expected to double the frequency of coastal flooding within the next decade, and some areas could experience floods of a magnitude 100 times higher than currently (Vitousek et al., 2017). People living in at-risk areas often ignore the impact of climate change on flood intensity and frequency. Immersive visual storytelling techniques proved promising and powerful tools to engage with and raise awareness of flood hazards. Here, we are introducing a framework to use Virtual Reality (VR) to reach better people living in coastal cities and …

Evaluation Of Native Plant Communities On Nebraska Game And Parks Commission State Parks, Robert F. Steinauer

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

This report evaluates the Native Plant Communities on Nebraska Game and Parks Commission State Parks East Region, also known as Region 3, which includes much of northeast and extreme eastern Nebraska, including portions of the lower Niobrara, Elkhorn and Platte Rivers and nearly the entire east border formed by the Missouri River. Fifteen state-listed threatened and endangered species have been recorded within the boundaries of Region 3 within the last twenty years. Several of these occupy habitats within major river channels.

Surveyed areas which contained habitat for legally protected species include Dead Timber State Recreation Area, Indian Cave State Park, …

Ronald E. Mcnair Scholars Program Profiles And Abstracts 2023, Motutama Sipelii

McNair Symposium

This is the complete event program and provides presentation abstracts and biographies of McNair scholars and their mentors.

Devices For Led Indicator Tamper Detection

Defensive Publications Series

Systems for LED indicator tamper detection using light characteristics are disclosed. In one embodiment an LED indicator assembly may include a light source within the LED indicator assembly configured to emit light having a known output light characteristic, a cover window to reflect a component of the light emitted by the light source, an ambient light sensor within the LED indicator assembly configured to receive the light emitted by the light source and background light, and one or more processors in communication with the light source and ambient light sensor, wherein the processors control the output light and compares the …

Rotunda - Vol 103, No 1 - Aug 16, 2023, Longwood University

Rotunda

No abstract provided.

Groundwater Resource Assessment For West Africa Using Earth Observations And Groundwater Modeling, Sergio Armando Barbosa Casas

Theses and Dissertations

Groundwater is a crucial global resource, particularly in semiarid regions where surface water is scarce. As a result, effective groundwater management is essential. However, the challenge lies in a lack of data and information for proper decision-making. This dissertation present studies related to groundwater storage changes and recharge in West Africa, using data from NASA's Gravity Recovery and Climate Experiment (GRACE) mission. In Niger, this research reveals little change in groundwater storage from 2002 to 2010 but a steep rise from 2010 to 2021, attributed partially to increased precipitation. Groundwater resources in Niger are found to be underutilized, with estimated …

Feasibility Of Rna-Based Pathogen Detection In An Infant Formula Matrix, Gweneth Claire Gustafson

Theses and Dissertations

Background: The dairy processing industry is currently hindered by slow, antiquated testing for high-risk pathogens, including Listeria monocytogenes and Cronobacter sakazakii. Powder infant formula's low water activity and substantial fat content enhance the survival and thermotolerance of such foodborne pathogens. As DNA-based detection methods can produce false positives even after cell death, a more accurate approach is needed. The objective of this study is to evaluate the suitability of an RNA-based platform for the detection of pathogens in a dairy powder matrix. Materials and Methods: A unique phenol-chloroform extraction method was used to obtain total RNA from heat-killed and unheated …

Brain-Derived Neurotrophic Factor Dysregulation As An Essential Pathological Feature In Huntington's Disease: Mechanisms And Potential Therapeutics, Andrew Speidell, Noman Bin Abid, Hiroko Yano

2020-Current year OA Pubs

Brain-derived neurotrophic factor (BDNF) is a major neurotrophin whose loss or interruption is well established to have numerous intersections with the pathogenesis of progressive neurological disorders. There is perhaps no greater example of disease pathogenesis resulting from the dysregulation of BDNF signaling than Huntington's disease (HD)-an inherited neurodegenerative disorder characterized by motor, psychiatric, and cognitive impairments associated with basal ganglia dysfunction and the ultimate death of striatal projection neurons. Investigation of the collection of mechanisms leading to BDNF loss in HD highlights this neurotrophin's importance to neuronal viability and calls attention to opportunities for therapeutic interventions. Using electronic database searches …

Office Of Research, Economic Engagement And Outreach Update, August 16, 2023, Office Of Research, Economic Engagement And Outreach

Office of Research, Economic Engagement and Outreach

No abstract provided.

Critical Engagements With Award Winning Picturebooks: My Journey In Creating A More Equitable Classroom Library, Carrie Elizabeth Crowe

Theses and Dissertations

Research consistently shows that students from historically marginalized populations have difficulty accessing texts that represent the realities of their lives. Concerned that such might be the case for my students, I conducted an inventory of my classroom library and sought out award-winning texts that could make my library more diverse and inclusive. I then analyzed these award-winning texts using Bishop's (1992) categories of multicultural literature in order to better understand the picturebooks' underlying messages and ideologies. My examinations showed that the majority of the texts fit into the same category (culturally specific), though I was able to identify award-winning texts …

Genome-Wide Association Studies And Fine-Mapping Identify Genomic Loci For N-3 And N-6 Polyunsaturated Fatty Acids In Hispanic American And African American Cohorts, Matthew C Pahl, Brian Hallmark, Yii-Der Ida Chen, Jason Westra, Lyn M Steffen, Christopher D Brown, David Siscovick, Michael Y Tsai, Alexis C Wood, Stephen S Rich, Caren E Smith, Timothy D O'Connor, Dariush Mozaffarian, Struan F A Grant, Floyd H Chilton, Nathan L Tintle, Rozenn N Lemaitre, Ani Manichaikul

Faculty, Staff and Students Publications

Omega-3 (n-3) and omega-6 (n-6) polyunsaturated fatty acids (PUFAs) play critical roles in human health. Prior genome-wide association studies (GWAS) of n-3 and n-6 PUFAs in European Americans from the CHARGE Consortium have documented strong genetic signals in/near the FADS locus on chromosome 11. We performed a GWAS of four n-3 and four n-6 PUFAs in Hispanic American (n = 1454) and African American (n = 2278) participants from three CHARGE cohorts. Applying a genome-wide significance threshold of P < 5 × 10

Demineralized Freeze-Dried Bovine Bone Xenograft Enhance Osteoblast Viability And Proliferation For Jaw Regeneration Materials, Feni Istikharoh, Sinta C. Wardani, Ariyati R. Pratiwi, Hidayat Sujuti, Edi Mustamsir

Journal of Dentomaxillofacial Science

Objectives The aim of this study was to analyze the viability and proliferation properties of the DFDBBX in MC3T3-E1 cell line Material and Methods Bovine was milled to reach nano sizes of 100-200 nm. We used 2 mg/mL, 4 mg/mL and 8 mg/mL to incubate MC3T3-E1 cells in the following experiment. Immunocytochemistry methods were performed using a Live/Dead cell imaging kit to investigate the viability of MC3T3-E1. To investigate the proliferation of MC3T3-E1, ICC was performed using an anti-Ki67 antibody. Using a confocal laser scanning microscope (CLSM), the cells were observed at LSIH Brawijaya University. Statistical analysis was performed using …

Labeling Piezo2 Activity In The Peripheral Nervous System, Nicholas W Villarino, Yasmeen M F Hamed, Britya Ghosh, Adrienne E Dubin, Amanda H Lewis, Max A Odem, Meaghan C Loud, Yu Wang, M Rocio Servin-Vences, Ardem Patapoutian, Kara L Marshall

Duncan NRI Faculty and Staff Publications

Sensory neurons detect mechanical forces from both the environment and internal organs to regulate physiology. PIEZO2 is a mechanosensory ion channel critical for touch, proprioception, and bladder stretch sensation, yet its broad expression in sensory neurons suggests it has undiscovered physiological roles. To fully understand mechanosensory physiology, we must know where and when PIEZO2-expressing neurons detect force. The fluorescent styryl dye FM 1-43 was previously shown to label sensory neurons. Surprisingly, we find that the vast majority of FM 1-43 somatosensory neuron labeling in mice in vivo is dependent on PIEZO2 activity within the peripheral nerve endings. We illustrate the …

The Pathophysiology Of Equine Glandular Gastric Disease: Investigating The Role Of The Mucosal Microbiome And Bile Acid Reflux, Linda Paul

LSU Doctoral Dissertations

Equine glandular gastric disease (EGGD) is a common disease of athletic horses that can affect performance and welfare. Clinical signs are non-specific and diagnosis is made using gastroscopy after a prolonged fasting period. Effective prevention and treatment strategies for EGGD are lacking despite its widespread prevalence and negative effects. The goal of this research was to investigate the pathophysiology of EGGD. Objectives included: 1) compare the gastric glandular mucosal microbiome in horses with and without EGGD, 2) assess the influence of dietary and management factors on the gastric microbiome, and 3) evaluate the pyloric gastric fluid bile acid profile in …

Enhanced Cerebroprotection Of Xenon-Loaded Liposomes In Combination With Rtpa Thrombolysis For Embolic Ischemic Stroke, Tao Peng, Keith Booher, Melanie R Moody, Xing Yin, Jaroslaw Aronowski, Sean I Savitz, Shao-Ling Huang

Faculty, Staff and Student Publications

Xenon (Xe) has shown great potential as a stroke treatment due to its exceptional ability to protect brain tissue without inducing side effects. We have previously developed Xe-loaded liposomes for the ultrasound-activated delivery of Xe into the cerebral region and demonstrated their therapeutic efficacy. At present, the sole FDA-approved thrombolytic agent for stroke treatment is recombinant tissue plasminogen activator (rtPA). In this study, we aimed to investigate the potential of combining Xe-liposomes with an intravenous rtPA treatment in a clinically relevant embolic rat stroke model. We evaluated the combinational effect using an in vitro clot lysis model and an in …

Platelet-Derived Exerkine Cxcl4/Platelet Factor 4 Rejuvenates Hippocampal Neurogenesis And Restores Cognitive Function In Aged Mice, Jocelyn Widagdo, Daniel G Blackmore, Perry F Bartlett, Victor Anggono, Saul A Villeda, Tara L Walker

Faculty, Staff and Student Publications

The beneficial effects of physical activity on brain ageing are well recognised, with exerkines, factors that are secreted into the circulation in response to exercise, emerging as likely mediators of this response. However, the source and identity of these exerkines remain unclear. Here we provide evidence that an anti-geronic exerkine is secreted by platelets. We show that platelets are activated by exercise and are required for the exercise-induced increase in hippocampal precursor cell proliferation in aged mice. We also demonstrate that increasing the systemic levels of the platelet-derived exerkine CXCL4/platelet factor 4 (PF4) ameliorates age-related regenerative and cognitive impairments in …

Dynamics Of Anti-Influenza Mucosal Iga Over A Season In A Cohort Of Individuals Living Or Working In A Long-Term Care Facility, Kyra H Grantz, Parker A Small, Keith R Fowke, Derek A T Cummings

Faculty, Staff and Student Publications

BACKGROUND: Serological surveys are used to ascertain influenza infection and immunity, but evidence for the utility of mucosal immunoglobulin A (IgA) as a correlate of infection or protection is limited.

METHODS: We performed influenza-like illness (ILI) surveillance on 220 individuals living or working in a retirement community in Gainesville, Florida from January to May 2018, and took pre- and postseason nasal samples of 11 individuals with polymerase chain reaction (PCR)-confirmed influenza infection and 60 randomly selected controls. Mucosal IgA against 10 strains of influenza was measured from nasal samples.

RESULTS: Overall, 28.2% and 11.3% of individuals experienced a 2-fold and …

Liposome-Based Carriers For Crispr Genome Editing, Shao-Ling Huang

Faculty, Staff and Student Publications

The CRISPR-based genome editing technology, known as clustered regularly interspaced short palindromic repeats (CRISPR), has sparked renewed interest in gene therapy. This interest is accompanied by the development of single-guide RNAs (sgRNAs), which enable the introduction of desired genetic modifications at the targeted site when used alongside the CRISPR components. However, the efficient delivery of CRISPR/Cas remains a challenge. Successful gene editing relies on the development of a delivery strategy that can effectively deliver the CRISPR cargo to the target site. To overcome this obstacle, researchers have extensively explored non-viral, viral, and physical methods for targeted delivery of CRISPR/Cas9 and …

Anti-Osteoclastogenesis Effects Of Gambir Extract Gel In Periodontitis, Hervina, Dwis Syahriel, Iga D. Haryani, Nlp Sm. Adnyasari, Haris Nasutianto

Journal of Dentomaxillofacial Science

Objective: RANK-RANKL-OPG signaling is being considered as the pathway periodontitis induces osteoclastogenesis. Alveolar bone resorption and increased osteoclast activity are both triggered by a greater RANKL/OPG ratio in periodontitis. Gambir contains catechins and quercetin, which have antimicrobial, antioxidant, and anti-inflammatory properties. This study aimed to analyze the anti-osteoclastogenesis effect of gambir extract gel through RANKL and OPG serum levels, the number of osteoblasts, and osteoclasts in alveolar bone tissue of Wistar rats. Material and Methods: Twenty-four male Wistar rats were divided into four equal groups, each receiving a different treatment: Group I received a placebo gel, Group II received Tetracycline …

Full Issue

Insights: The Newsletter of the Neal A. Maxwell Institute for Religious Scholarship

No abstract provided.

Hundreds Attend Farms Symposia

Insights: The Newsletter of the Neal A. Maxwell Institute for Religious Scholarship

On March 23, some 700-800 people from Utah and other parts of the country turned out to hear presentations about the ancient scrolls and how they contribute to LDS understanding of gospel teachings, the Bible, and other aspects of biblical life. The symposium was sponsored by BYU Religious Education and FARMS.

Militias, Manhood, And Citizenship In Reconstruction Mississippi, 1868-1875, Jacob S. Clawson

Journal of Mississippi History

No abstract provided.

01. Animating Traditional Stories As Pedagogical Tools For Language Reclamation (P, T), Noah Ha'alilo Solomon, Dannii Yarbrough

CoLang 2022 Workshops

In this hands-on workshop, participants will learn practical skills for creating animated videos of traditional stories for use in community language reclamation efforts. Curriculum centered within Indigenous storytelling prioritizes place-based education and alternative literacies that are essential to Indigenous knowledge systems while reinforcing the validity of Indigenous traditions of language transmission and destabilizing the hegemony of Western pedagogical practices. Each day we will begin with a presentation and discussion of different topics from Indigenous scholarship in pedagogical methods as they relate to developing appropriate animated videos, including linguistic features of narratives, and potential applications of animated traditional stories in language …