Digital Commons Network^™

Characterization Of Host Cell Specific Interactions During Spotted Fever Group Rickettsiae Infection, Emerson Towey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Vector-borne diseases have been increasing over the past few years due to changes in climate and disturbances in the vectors’ natural environment. To mitigate the risks of these diseases, it is necessary to understand pathogen biology and how it interacts with a host to cause disease. Here we focus our attention on the tick-borne pathogen, Rickettsia rickettsii. R. rickettsii is a member of the spotted fever group (SFG) Rickettsiae and is the etiological agent of Rocky Mountain spotted fever (RMSF), a fatal human disease that is lacking an effective vaccine. Members of the bacterial genus Rickettsia are obligate intracellular endoparasites …

Impact Of Tiered Implementation Of Clinical Decision Support System For Acute Kidney Injury And Nephrotoxin Exposure In Cardiac Surgery Patients, Christopher M. Justice Ii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Nephrotoxin exposure may worsen kidney injury and impair kidney recovery if continued in patients with acute kidney injury (AKI).

Objective: To determine if tiered implementation of a clinical decision support system (CDSS) would reduce nephrotoxin use in cardiac surgery patients with AKI.

Methods: We assessed patients admitted to the cardiac surgery intensive care unit at a tertiary care center from January 2020 to December 2021, and August 2022 to September 2023. A passive electronic AKI alert was activated in Jul 2020, followed by an electronic nephrotoxin alert in March 2023. In this alert, active nephrotoxic medication orders resulted in …

An Evaluation Of A Human-Operant Effort Manipulation And Effects Of Effort Disparity On Renewal, Haleh Amanieh

Graduate Theses, Dissertations, and Problem Reports (ETD)

The relative effort of target and alternative responses during treatments using differential reinforcement of alternative behavior may impact the likelihood that a previously reduced target response will reemerge following a context change (i.e., “renewal”). The purpose of this study was to evaluate the role of an effort disparity between target and alternative responses in a human-operant arrangement. Eighteen college students clicked on one (Experiment 1) or two (Experiment 2) circles moving on a computer screen for points. In Experiment 1, the speed of the circle was manipulated as an index of effort such that three circle speeds (i.e., 50, 100, …

Risk Of Parasiticides To Dung-Associated Beetles In West Virginia, Haylie J. Brown

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ecosystem services provided by dung-associated beetles, such as reducing pasture fouling, parasite suppression, supporting nutrient recycling, and increasing water infiltration, contribute to animal and pasture health. However, parasiticides used to manage cattle parasites, particularly flies and helminths, in West Virginia, can adversely affect these non-target beetles. We assessed the exposure and impact of some parasiticides used in WV including Clarifly^® (active ingredient [a.i.] diflubenzuron), Cydectin^® (a.i. moxidectin), LongRange^® (a.i. eprinomectin), and Safe-guard^® (a.i. fenbendazole). In Chapter 1, we review the use of parasiticides in the US and their non-target effects on beneficial dung-associated beetles. In Chapter …

Implementing A Nurse-Driven Clostridium Difficile Screening Protocol, Sandrela Magnuson

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Background: Clostridium difficile infection (CDI) is a prevalent infection often contracted in hospitals. CDI is also increasingly originating from community settings. In addition to the health risks associated with CDI, diagnosis of a hospital-acquired CDI can have a significant financial impact on hospital systems. When hospital acquired, hospital systems are no longer reimbursed by payers. Secondary to the increasing prevalence of community-acquired CDI, mechanisms for early detection and isolation are warranted.

Local Problem: J.W. Ruby Memorial Hospital has an integrated Clostridium difficile (C. difficile) electronic health record (EHR) screening questionnaire that requires updating to align with current guidelines.

Purpose: …

Role Of Β4b-Tubulin (Tubb4b) In The Sensory System And Spermatogenesis, Urikhan Sanzhaeva

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microtubules are ubiquitous cytoskeletal filaments essential in the function of eukaryotic cells. These polymers, comprised of αβ-tubulin heterodimers, are critical for cell division, intracellular transport, and formation of the axonemes of cilia and flagella. This functional diversity of microtubules is attributed to the incorporation of various α- and β-tubulin isotypes encoded by different genes. Indeed, mutations in human tubulin genes have been linked to various tissue-specific diseases, suggesting a unique function for each tubulin isotype. Mutations in the β4B-tubulin gene (TUBB4B) are associated with sensorineural hearing loss, Leber Congenital Amaurosis (LCA), a severe childhood blindness, and primary ciliary dyskinesia …

The Effects Of Feed Manufacture On Exogenous Enzymes, Starch Gelatinization, And Antinutritional Factors Associated With Soybeans, Kristina M. Bowen

Graduate Theses, Dissertations, and Problem Reports (ETD)

This doctorate dissertation consists of four individual studies with the overarching theme of the effect of feed manufacture on exogenous enzymes and antinutrients found in soybeans.

Study 1. Including exogenous enzymes in poultry diets can liberate nutrients that are typically inaccessible to monogastric animals. Phytase feeding research has traditionally been conducted by rearing broilers in raised wire cages, whereas commercially grown broilers are reared on built-up litter. Therefore, cage-based studies may not accurately represent commercial bird performance response to phytase. Birds with access to litter consume it, possibly increasing phosphorus intake leading to suggestions that lower phytase concentrations are required. …

Astrocyte Reprogramming By Th17 Cells In Autoimmune Neuroinflammation: Mechanisms And Implications, Sarah Marie Milne

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multiple sclerosis (MS) is a cell-mediated, demyelinating autoimmune disease that affects the central nervous system (CNS). T helper (Th) 17 cells mediate the pathogenesis of MS and its animal model, experimental autoimmune encephalomyelitis (EAE), in part by infiltrating the CNS and interacting with astrocytes, a resident glial cell. Astrocytes are central to coordinating several inflammatory processes in the CNS and can respond to cytokines and chemokines produced by encephalitogenic Th17 cells. Pathogenesis of MS and EAE requires the infiltration of immune cells from the periphery, including autoreactive T cells, B cells, monocytes, and neutrophils, that lead to neuroinflammation, demyelination, and …

Advanced Techniques In Time Series Forecasting: From Deterministic Models To Deep Learning, Xue Bai

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation discusses three instances of temporal prediction, applied to population dynamics and deep learning.

In population modeling, dynamic processes are frequently represented by systems of differential equations, allowing for the analysis of various phenomena. The first application explores modeling cloned hematopoiesis in chronic myeloid leukemia (CML) via a nonlinear system of differential equations. By tracking the evolution of different cell compartments, including cycling and quiescent stem cells, progenitor cells, differentiated cells, and terminally differentiated cells, the model captures the transition from normal hematopoiesis to the chronic and accelerated-acute phases of CML. Three distinct non-zero steady states are identified, representing …

The Effects Of Diet-Induced Obesity On Cerebral Microvascular Bioenergetics, Alec Anthony Hanshew

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obesity is a worldwide epidemic but is especially a concern in the United States with over 40% of the population being obese. Obesity is known to cause increased metabolic demand, systemic inflammation, peripheral vascular dysfunction, and increased levels of oxidative stress and reactive oxygen species (ROS). However, the role of obesity on cerebrovascular bioenergetic function has not been fully elucidated. This study aimed to determine the impact of diet-induced obesity on cerebrovascular bioenergetics. 72, 6-month-old C57Bl/6 male and female mice were randomized into control and HFD groups. Control mice were subjected to the feeding of standard chow (18% fat, 24% …

Translational Approaches To Address The Metastatic/Resistant (Mr) Phenotype Of Non-Small Cell Lung Cancer Brain Metastases, Kent L. Marshall

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lung cancer remains the most frequently diagnosed malignant neoplasm shared in men and women worldwide, second only to the sexually dimorphic prostate cancer in men and breast cancer in women. Overall, lung cancer was responsible for 12.5% of new cancer cases globally in the year 2022. Of these cases, 80-85% are non-small cell lung cancer (NSCLC), a highly genotypically diverse sub-type of lung cancer. Over the past three decades, extraordinary improvement in screening, smoking cessation, early diagnosis and targeted therapy have been made, improving NSCLC survival markedly. However, as patients are surviving their primary disease better, there is a temporal …

The Identification And Characterization Of Novel And Improved Antigens For Next-Generation Pertussis Vaccines, Gage Michael Pyles

Graduate Theses, Dissertations, and Problem Reports (ETD)

Whooping cough, or pertussis, is a highly infectious respiratory disease caused by infection with the Gram-negative bacterial pathogen Bordetella pertussis. Pertussis affects all age groups and populations; however, it is most severe in infants who are too young to receive vaccinations and remains a global health concern with more than­­­­ 200,000 cases reported globally in 2023. Vaccines against pertussis have been available in the United States since the 1940’s with the development and widespread use of whole cell pertussis vaccines. Following their introduction, the incidence of pertussis was controlled and limited to fewer than 5,000 cases per year. Despite …

Performance And Physiological Impacts Of Branched-Chain Amino Acid And Tryptophan Ratio Variations In Turkey Starter Diets Utilizing Concentrated Corn Proteins, Emily Blair Estanich

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, a study was designed to evaluate the effects of varying dietary branched-chain amino acids (BCAA) ratio in corn and soybean meal-based turkey starter diets. The BCAA leucine, isoleucine, and valine are considered indispensable amino acids needed for energy and stimulation of muscle protein synthesis via the mammalian target of rapamycin (mTOR) pathway. Imbalanced BCAA ratios can lead to antagonism and degradation of limiting BCAA, particularly in diets with excess leucine. Concentrated corn proteins contain high levels of leucine and can alter BCAA requirements; however, crystalline BCAA may be added to correct these ratios. This study aimed to …

Cell-Type-Specific Effects Of Synaptic Zinc In Mouse Auditory Cortex, Mason Mccollum

Graduate Theses, Dissertations, and Problem Reports (ETD)

Synaptic zinc signaling modulates synaptic activity and is present in specific populations of cortical neurons, suggesting that synaptic zinc contributes to the diversity of intracortical synaptic microcircuits and their auditory tuning properties. Stimulus-specific adaptation is a hallmark of sensory processing in which a repeated stimulus results in diminished successive neuronal responses, but a deviant stimulus will still elicit robust responses from the same neurons. Recent work has established that synaptically released zinc is an endogenous mechanism that shapes neuronal responses to sounds in the auditory cortex. Here, to understand the role of zinc signaling in the auditory cortex and the …

The Effects Of Forgetting And Disclosure On Mock Jurors' Perceptions Of An Alleged Rape, Johnathan Meier

Graduate Theses, Dissertations, and Problem Reports (ETD)

Memory recall and forgetting is a complex process. Memories can be completely forgotten (e.g., Ebbinghaus, 1885/1964; Murre, 2015) or repressed and recovered at a later time (e.g., Key et al., 1996; Foster-Lee et al., 1999). Instances of sexual assault may be disclosed to a close acquaintance or medical professional at the time of the event or kept secret until the time of reporting to authorities (e.g., Pica et al., 2020a). In the current study, mock jurors’ perceptions of credibility and guilt, as well as the amount of compensation awarded to the plaintiff, will be assessed in response to testimony depicting …

Qualitative Interviews, Abraham Kenin

Research Datasets

Background: Within the context of a growing sub-Saharan Africa (SSA) population in Australia, the problem of alcohol and other drug (AOD) use by SSA youth has been reported in recent studies. Although evidence from other sociocultural contexts indicate that SSA cultural values could be protective against youth AOD use, not much is known about what pertains in the Australian context. The core justification for conducting this study is grounded on the awareness that people may perceive and conceptualize their heritage cultural values based on the sociocultural context in which they find themselves (Akkuş et al., 2017).

Objectives: To explore SSA …

Marine Heatwaves And Impacts On Marine Ecosystems, Anna Lafratta, Hollie Williams, Stanley Mastrantonis, Sharyn Hickey, Kathryn Mcmahon, Arani Chandrapavan, Andi Laidlaw, Emily Lester

Research Datasets

The metadata is a compilation of existing long-term datasets available across Western Australia coastline. This was prepared as an initial step in identifying what is known about the Western Australia marine ecosystem and to explore the responses to marine heatwaves and for future planning. For each dataset a series of information are provided, including Institute that collected the data and contact details, period and frequency of data collection, Department of Primary Industries and Regional Development (DPIRD) bioregion, habitat and species studies, variables analysed and methods of data collection. Seventeen organizations contributed to these metadata records. The details of these datasets …

An Experiment To Determine Mineral-Associated Organic Matter Turnover In A Sandy Clay Loam, Samantha Abel

All Master's Theses

Soil is an important terrestrial carbon sink. Through regenerative land management, which includes minimizing soil disturbance, the carbon content of soil can be raised towards preindustrial levels. This feat requires effectively storing carbon in a natural system that is inclined to cyclicity. The most stable form of soil organic matter is mineral-associated organic matter (MAOM). This carbon labelling experiment was designed to identify short-term CO₂ fluxes within the soil-plant-atmosphere system, particularly those related to MAOM turnover in the rhizosphere. Basil (Ocimum basilicum) was planted on a plot at the campus farm at Central Washington University. Another plot …

The Surface Chemistry And Binding Interactions Of Lignin With Polymer-Encapsulated Gold Nanoparticles Acting As Model Microplastics, Oluwaseun Ayodeji Akinsola

All Master's Theses

This study investigates the surface chemistry and molecular-level interactions between lignin, a special type of natural organic matter, and polymer-capped gold nanoparticles, shedding light on the strength of adsorption between lignin and nanoscale polymer surfaces. Specifically, the study presents a variety of nanoscale polymer surfaces displaying different charged functional groups, using layer-by-layer assembly of three polyelectrolytes (polyallylamine hydrochloride (PAH)), polyacrylic acid (PAA), and poly(diallyldimethylammonium chloride (PDADMAC)) on 90 nm citrate-stabilized gold nanoparticles (AuNPs). This approach provides a library of polymer-encapsulated AuNPs for investigating the binding interactions of lignin to nanoscale polymers via spectroscopic techniques. ζ-potential, dynamic light scattering (DLS), …

Factors Associated With The Prevalence Of Iron Deficiency And Anemia In Women Of Reproductive Age In The U.S.: Nhanes 2015-2020, Catherine Wise

All Master's Theses

Iron deficiency (ID) is well recognized as the most prevalent nutrient insufficiency and a leading cause of anemia. Women of reproductive age (WRA), females 15 to 49, are at an increased risk of ID and anemia. This is due to the heightened iron demands of this life stage, including puberty, menstruation, and pregnancy, which require iron intakes ranging from 15 to 27 mg/day. Poor health and nutrition increase the risk of ID and progression to anemia. Consequences of ID and anemia range from exhaustion and decreased cognitive function to an increased risk of mortality. This study aimed to assess the …

Gnss Radio Propagation Through Trapped Atmospheric Lee Waves In The San Bernardino Valley, Ca, Logan Grey

All Master's Theses

Atmospheric lee waves, also known as mountain waves, are a type of gravity wave that form as air that is forced over a mountain creates turbulence downstream. Trapped, or stationary, lee waves located directly over a Global Navigation Satellite System (GNSS) receiver on Earth’s surface appear to lead to anomalies in the receiver’s position estimate, usually skewed toward the neighboring mountain range. The exact mechanism by which trapped lee waves might cause these anomalies is not known, and so my research aims to understand this. GNSS station P612 located in the lee of the San Bernardino Mountains in southern California …

Investigating A Potential Protein-Protein Interaction Between Transcription Factor Pax6 And Ribosomal Protein S20 (Rps20), Modupe Oladayo

All Master's Theses

The neocortex is the largest region of mammalian brains and is responsible for the complex cognitive abilities and behaviors of mammals. The neocortex of mature mammals is divided into concise areas, which process sensory information from ears, eyes, and touch receptors, and regulate conscious movement of muscles. The combined graded expressions of several transcription factors over the embryonic neocortical ventricular zone regulates the sizes of these functional areas in the adult neocortex. Although progress has been made in understanding how transcription factors affect neocortical patterning, additional research is required to fully understand the underlying molecular mechanism(s), which will contribute to …

Comparing The Prevalence Of Stereotypic Behaviors And Enrichment Use In Rhesus (Macaca Mulatta) And Japanese Macaques (M. Fuscata), Jesscia Oscavich

All Master's Theses

To improve captive nonhuman primates’ welfare, particularly those utilized in biomedical and behavioral research, it’s important to understand their behavior. Rhesus (Macaca mulatta) and Japanese macaques (M. fuscata) are two closely related species that are commonly utilized in biomedical and behavioral research. In this study, I compared the frequency of stereotypic behaviors, which are abnormal repetitive behaviors that are apparently non-functional, and enrichment use among four groups of each species. Stereotypic behaviors were divided into motor, which the animal performed while active, and self-directed stereotypies, which the animal performed towards themselves. Enrichment use was divided into …

Prenatal Androgen Exposure Reduces Ovarian Function, Marlee Weets

All Master's Theses

Polycystic ovary syndrome (PCOS) is the most common reproductive and endocrine disorder, being the leading cause of infertility for females of reproductive age. The etiology of PCOS is not fully known, although it is hypothesized that there is a genetic, epigenetic, and environmental component to the disease. One of the environmental influences that can cause an abnormal reproductive phenotype similar to PCOS is prenatal exposure to excess androgens (male sex hormones). Previous studies have shown that prenatal exposure to androgens in mice can cause a PCOS-like ovarian phenotype similar to people with PCOS. To examine how prenatal androgen exposure causes …

The Auricular Surface Of Chimpanzees (Pan Troglodytes) As An Indicator Of Age, Kalila Garrison

All Master's Theses

Great ape skeletal remains are scarce and their use in biological profile estimations has been limited. Skeletal remains analysis provides key demographic information and ecological context to the individual. Chimpanzees (Pan troglodytes) share skeletal morphological characteristics with humans, and this study seeks to understand if two noninvasive methods using the auricular surface of the ilium to age human skeletal remains could be applied to chimpanzees. The sample consisted of n = 8 individual left auricular surfaces from known age groups of both sexes. Firstly, the Buckberry and Chamberlain Method assigns numerical values to five different traits on the auricular …

Musical Instruments From Recycled Materials : A Case Study Of Uthen Pialor, Vich Boonrod, Chananya Piratanatsakul

Journal of Urban Culture Research

This research article focuses on two objectives: examining the design concepts and construction techniques of musical instruments made from recycled materials by Uthen Pialor and exploring the materials used and their associated benefits. The researcher utilized a case study research design, conducting qualitative research through semi-structured interviews. The interviews covered three key aspects: design concepts, construction techniques, materials used, and benefits derived from using recycled materials. Findings revealed innovative design concepts, prioritizing sturdy recycled materials and integrating natural elements. Uthen Pialor successfully created 15 instruments across four types using everyday materials. Benefits included addressing instrument scarcity, increasing waste material value, …

The Development Of Sustainability For Tonkori Performance Of The Ainu People In Northern Japan, Kumkom Pornprasit

Journal of Urban Culture Research

The Ainu are the indigenous people of the northern region of Japan which is adjacent to the Russian border. An important Ainu musical instrument is the Tonkori, a wooden 5-string instrument played for entertainment purposes. The current Tonkori music is considered to be introduced by Sakhalin Ainu, one of the sub-ethnic groups of Ainu who migrated to Hokkaido prefecture after WWII. An effort to reduce inequality as well as to make their culture sustainable among the Ainu people is the followings: (1) Promoting the establishment of university research centers; (2) organizing workshops and musical performances in museums and recital halls; …

Historical Narratives Of The Eight Akan Clan Systems Using Museum Theater : The Case Of Prempeh Ii Jubilee Museum, Ghana, Emmanuel Jewel Peprah Mensah, Dickson Adom, Steve Kquofi

Journal of Urban Culture Research

There have been several calls from diverse stakeholders in the museum sector in Ghana for creative programs that enliven the cultural objects in a museum’s holding. This study aimed at exploring the use of museum theatre as an intervention to enhance the museum experience of visitors at the Prempeh II Jubilee Museum, a popular museum in the Ashanti Region of Ghana. We produced The Linguist Staff, an interactive drama to present the historical narratives of the eight Akan clan systems. The study was carried out using arts-based research under the qualitative approach with observer-as-participant and semi-structured interviews as data collection …

Krung Curatorial Practice And Creative Sustainability For Ethnic Music Of Mountainous Villagers In Nan Province, Pornprapit Phoasavadi

Journal of Urban Culture Research

The objective of this project is to explore the collaborative methods in preserving the wealth of krung, one of musical resources in Nan province. The curatorial and collaborative methods engaged with the process of developing plans together with artisans, testing the model with stakeholders, adapting to learn the lessons and mistakes in the field, and to implement a longitudinal activity to integrate cultural and biological ecology. Active agents are the only two instrument makers in the village who possess local wisdom of plant identification, wood cutting, forest hiking. Stakeholders are the local administration of the village, local organization of water …

Assessing The Niger Delta Residents’ Awareness On Socio-Culture Effect Of Wetland Loss, Faith Danjuma, Özge Özden Fuller, Salih Gucel, Patience Francis Nathan

Journal of Urban Culture Research

The degradation of Niger Delta ecosystem is due to activities such as crude oil exploitation, agricultural activities, urbanization among others that affects the socio-culture impact of its dwellers. The study's aim was to assess the Niger Delta residents’ awareness on socio-culture effect of wetlands loss. This paper engaged the exploration of literature reviews, the randomly selection of communities in three Niger Delta states which includes Rivers, Bayelsa and Delta state and a questionnaire was utilized with 150 participants. The findings demonstrated that less awareness on the effect of wetland loss has impacted the residents. Also industrial activities have affected the …