Digital Commons Network^™

Temporal Distribution Of Potential Waterborne Pathogens In Sequiota Spring, William J. Durstock

Graduate Theses/Dissertations

Waterborne pathogens from human fecal matter pose a significant health risk in karst environments, where domestic wastewater can easily be transferred to the shallow groundwater system via old, leaky septic tanks and damaged sewer lines. This study monitored fecal indicator bacteria (FIB) and potential pathogens in Sequiota Spring located in the karst region of southwest Missouri, using Microbial Source Tracking (MST) and Illumina DNA sequencing of bacterial 16S rRNA genes. MST identified high levels of human fecal indicator bacteria (HFIB) (up to 110,000 cells/L) in July 2020. By June 2022, HFIB levels had dropped significantly (55-fold reduction) after the City …

Effects Of Subchronic Oral Pfos Exposure Via Drinking Water On Gut Microbiota Composition, Emma Ann Loder

Graduate Theses/Dissertations

Perfluorooctanesulfonic acid (PFOS) has been phased out of production in the United States, but because of its resistance to degradation and its persistence in the environment, PFOS can be found in drinking water across America. Oral exposure to PFOS has been linked to adverse developmental, immunological, hepatic, and endocrine health. Oral PFOS exposure may alter the gut microbiota and cause dysbiosis, which is implicated in the pathology of inflammatory diseases like Crohn’s disease, diabetes, and ulcerative colitis. The goal of the study was to investigate the effects of subchronic oral PFOS exposure on the composition of the gut microbiota. Adult …

Microvascular Endothelial Permeability Coefficient Regulated By Purinergic P2y2 Receptor, Dana S. James

Graduate Theses/Dissertations

Inflammatory mediators profoundly affect endothelial permeability by disrupting its barrier function, resulting in increased permeability (hyperpermeability). Interestingly, hyperpermeability is involved in a wide range of disorders and diseases, and can pose significant risks to life in diseases, such as acute respiratory distress syndrome and systemic capillary leak syndrome. Though vascular leakage, a hallmark of inflammation, profoundly exacerbates the pathogenesis of diseases, the direct mechanism is not fully understood. In vivo study has shown that activation of nucleotide P2Y₂ receptor (P2Y₂R) increases permeability in murine venule. The goal of this study was to determine the molecular mechanism of …

Patterns Of Epiphytic Bacterial Growth On Flower Surfaces: Relationships With Flower Longevity And Environmental Factors, Rita Nkem Afagwu

Graduate Theses/Dissertations

Flowers host diverse communities of epiphytic bacteria, which can impact floral functions, pollinator interactions, and overall plant fitness. However, little is known about how the abundance of these bacteria change as flowers age, and how these shifts may correlate with floral longevity. In this study I investigate the change in bacterial abundance across the lifespan of a flower from anthesis (opening of bud to flower) to senescence (wilting of flower) and to explore the potential implications for floral longevity. We tracked the age of the flowers by determining the average age to senescence in flowers of 8 plant species over …

Sptbn1 Involvement In Reverse Transcription Of Hiv-1 Infection In Chme3 Cells, Jeanette Drecker

Graduate Theses/Dissertations

Human Immunodeficiency Virus 1 (HIV-1) is a lentivirus that infects CD4+ cells, causing a

weakened immune system that leads to the development of Acquired Immunodeficiency

Syndrome (AIDS). HIV utilizes host proteins to replicate. A genome wide screen by Brass has

shown that the molecular scaffolding protein, SPTBN1, is essential for HIV-1 infection.

However, the viral replication steps that SPTBN1 impacts are not known. Previous work in the

Hulme Lab has shown that SPTBN1 knockdown delays HIV capsid uncoating but does not

impact viral fusion kinetics. Uncoating and reverse transcription of HIV-1 occur simultaneously.

Therefore, the goal for this study was …

Water Quality Of A Karstic Shallow Aquifer In Greene And Lawrence Counties, Missouri, Claudia Patricia Colmenero Chacon

Graduate Theses/Dissertations

A water quality investigation in the Springfield Plateau aquifer of Greene and Lawrence counties, Missouri, was conducted. Water samples from eleven springs were collected at regular intervals during 2023 to determine major water quality parameters: total dissolved solids (TDS), alkalinity, calcium (Ca), magnesium (Mg), sodium (Na), sulfate (SO₄), chloride (Cl) and nitrate-nitrogen (NO₃-N), along with per- and poly-fluorinated alkali substances (PFAS) from spring sediments. Nitrate-nitrogen was analyzed because of the importance for environmental health (e.g. to avoid eutrophication). Springs samples were separated by land use into urban and rural samples. In addition, Springfield Plateau water quality …

Demographics And Recruitment Patterns In A Reintroduced Population Of Alligator Snapping Turtles (Macrochelys Temminckii), Melisa P. Blasky

Graduate Theses/Dissertations

Reintroductions have increasingly become a useful tool for managing wildlife populations, but post-reintroduction studies to evaluate the success of these efforts are often lacking. In 2007, 265 Alligator Snapping Turtles (Macrochelys temminckii) were released into several semi-impounded wetlands in the Washita River drainage basin in southeastern Oklahoma, where surveys in the late 1990s suggested that the species was functionally extirpated. I conducted turtle trapping surveys using baited hoop nets to assess demographics and recruitment patterns in the population 11–12 years after the initial reintroduction. I estimated the age of juveniles by counting scute annuli and compared the data …

How Environmental Variation Affects Bumblebee Cognition And The Gut Microbiome, Parker Madison Campbell

Graduate Theses/Dissertations

This master's thesis examines the influence of environmental variables on cognitive processes in Bombus impatiens, focusing on the effects of ingested microbial communities on gut microbiota and their subsequent impact on associative color learning. The study implemented controlled feeding trials with isolated microbe species (Candida bombi, Metschnikowia reukaufii, Lactobacillus micheneri and a control with no microbes) to analyze shifts in gut microbial composition in foraging bees. Results indicated minor alterations in the microbial profiles of bees, yet no statistically significant correlation was found between these modifications and variations in learning or memory performance. Furthermore, the study assessed the impact of …

33. Chatgpt Assistance In Creating Chemistry Practice Problems: Pitfalls, Positives, And Possibilities, Michael A. Christiansen

Teaching and Generative AI: Pedagogical Possibilities and Productive Tensions

ChatGPT, a relatively new Large Language Model (LLM) artificial intelligence (Al) chatbot, has gained significant attention as the most downloaded software app, accruing over 100 million users within two months of its release. This software can generate quick, articulate responses to virtually any textual query. Many educators are concerned about its potential for enabling student cheating. However, it currently suffers significant limitations in solving chemistry problems-documented through peer-reviewed articles and from the author's experience suggesting that chemistry students will perform far better, on average, by studying than by relying on ChatGPT. This is particularly true of math-centric problems, which the …

The Role Of Prejudice In Political Campaigns, Sara Steffens

A with Honors Projects

No abstract provided.

The Protective And Pathological Role Of The Spleen In Malaria, Julie Hwang, Md, Amy Dechet

Books, Presentations, Posters, Etc.

Case Presentation: • Background: There were 241 million cases of malaria globally. Compared to 2,000 cases in the U.S. Splenomegaly has previously been used to determine the severity of malaria. This is a rare case of hyper reactive malarial splenomegaly (HMS) due to chronic plasmodium ovale • 23 year-old female with gradually worsening left upper quadrant abdominal pain of 1 month. Pain sharp and stabbing in quality, no alleviating or aggravating factors. Associated with subjective early satiety and 10 Ibs weight loss, generalized weakness, shortness of breath • PMH: Schistosomiasis 2008,unclear if treated • Soc: Born in Congo and immigrated …

Impulsive Behavior In The Context Of A Substance Use Treatment Seeking Sample: A Conditional Process Analysis Of Childhood Maltreatment, Experiential Avoidance, And Cognitive Fusion, Emily Kalantar

Dissertations and Theses

Substance misuse is associated with high impulsive behavior and may represent one behavioral manifestation of impulsive behavior. One predictor of substance misuse is childhood maltreatment, which is, in turn, associated with difficulty regulating unwanted emotion. Accordingly, impulsive behavior is high among individuals exposed to maltreatment and likely functions as one behavioral strategy for coping with unwanted internal experiences. Consequently, identification of behavioral processes that may account for the childhood maltreatment and impulsive behavior relation in substance misusers appears warranted. One process-based behavioral model that may be useful in this endeavor is psychological flexibility, which includes the inflexible processes of experiential …

More Than A Stop On The Way To Yellowstone: The Parks Of The Black Hills And Their Impact On Wilderness Preservation, Arianna Lynn Van Kley

Dissertations and Theses

This thesis examines the ideologies that contributed to the establishment of public parks in the Black Hills of South Dakota as a microcosm for nationwide conversations about preservation, tourism, nationalism, colonialism, and commerce. Wind Cave National Park, Custer State Park, and Mount Rushmore National Memorial each complicate and enhance understandings of how public preservation projects emerged out of debates that included ideas outside environmental protection efforts. The people who supported the establishment of these parks, such as the McDonald and Stabler families, South Dakota Senator Peter Norbeck, state historian Doane Robinson, and sculptor Gutzon Borglum, implemented their own ideas across …

The Effects Of Bilateral Stimulation On Motor Learning, Shania Rehmudin

Dissertations and Theses

Motor learning is a set of complex processes aimed at learning and refining new skills by practicing them. There are three proposed stages of motor learning: cognitive, associative, and autonomous. While all stages are crucial, research suggests that the cognitive stage holds particular significance, laying the foundational groundwork for effective skill acquisition. Although research has explored many innovative ways to facilitate motor memory, to our knowledge, none has explored the optimization of motor skill learning through manipulating the focus of attention using bilateral stimulation. Therefore, this study explored the effects of bilateral stimulation on squat skill acquisition. Nineteen (19) subjects, …

2024-2025 4-H Ag Ambassador Interest Packet, Hannah Majewski

UNH Cooperative Extension

No abstract provided.

To Swallow The Sun, John Morris Cox

Selected Full-Text Master Theses 2021-

My creative fiction thesis To Swallow the Sun is part one of a novel of the same name. Cage, the protagonist, is a dreamer; he yearns for visceral experience and enjoys assignations with the night beyond his bedroom window; but as his desires draw him deeper into a world of his own, there are many who see nothing but a boy in need of fixing. After continually falling short of the expectations his family and society have crafted for him- abandoning work, “ruining” school plays, even failing to react to a prisoner’s execution- Cage is sent to a psychiatric ward. …

Nsp3 Protein-Portion Interaction Of Sars-Cov-2 Using Two Yeast-Hybrid System, Ghadeer Jassem Zakareya

Selected Full-Text Master Theses 2021-

The NSP3 protein of SARS-CoV-2 plays a crucial role in the virus-host interaction during infection. This thesis explores the protein-protein interactions between NSP3 and host factors, shedding light on their significance in viral replication and pathogenesis. NSP3 is known to interact with various host proteins involved in key cellular processes, including innate immune response modulation, RNA processing and protein degradation pathways. Understanding these interactions is essential for developing targeted therapeutic strategies against COVID-19. Application of The Matchmaker GAL4Two-Hybrid Systems, to isolate the NSP3 protein and study the interactions, elucidating the NSP3 host protein interactions and attempting to identify potential key …

Characterizing Sex Differences In Hpv Negative Head And Neck Squamous Cell Carcinoma, Sarah Barclay

Dissertations and Theses

It is now widely accepted that peripheral solid tumors are infiltrated by nerves. This infiltration of malignancies by nerves is referred to as tumor innervation. The Vermeer laboratory studies tumor innervation primarily in head and neck squamous cell carcinoma using a syngeneic cell line representing a mutationally-induced form of the disease; these cells are called MOC2-7 cells. In addition to defining the molecular consequences of tumor-infiltrating nerves on disease, the lab also focuses on their influence on behavior. This is important as cancer patients have a significantly higher incidence of depression and anxiety as compared to the population at large. …

Debunking Some Myths And Misinformation About Africa And Africans, Michael Oke-Ndukwu

A with Honors Projects

This project lists some misinformation about Africa and compares that misinformation to factual evidence.

The 6888th Central Postal Directory Battalion And How Black Women Contributed To America, Valencia Crosby

A with Honors Projects

For this project, I chose to read the novel, No Better Time by Sheila Williams. This novel was published and distributed in 2024 and has had raving reviews from countless magazines/journals such as Writer's Digest, The Root, Historical Novel Society, and the Atlanta Journal-Constitution.

Addressing Spirituality In The Counselor Supervision Relationship, Andrew H. Gerodias

Dissertations and Theses

A qualitative, phenomenological study was implemented to investigate the experiences of independently licensed supervisors who oversee counselors who utilize the 12 steps in the work done with those dealing with substance use disorders. Seven participants from the Midwest completed an initial semi-structured interview, journaling activity, and follow-up interview. Data was subjected to member checks and an external audit to ensure themes were trustworthy. Two overall textural-structural themes were found through the integration of the initial nine themes developed; five textual themes describing what was experienced (acknowledging resistance and consideration, using spirituality as a tool, acknowledging differences and diversity, observed growth …

Exploring The Role Of Nonmuscle Myosin Ii In Renal Thick Ascending Limb Epithelial Cell Protein Trafficking And Organelle Architecture, Brook Busselman

Dissertations and Theses

The traditional functional roles of the actin associated motor protein nonmuscle myosin II (NM2) have been thoroughly studied. However, the nontraditional roles for NM2 in the context of membrane remodeling and protein trafficking events in cells have just recently been unraveled. Our work has found that genetic inactivation of NM2 protein isoforms MYH9 and MYH10 in the renal epithelium of adult mice leads to progressive kidney disease. Aberrant protein transport and localization in cells of the thick ascending limb (TAL) of the kidney nephron is accompanied by upregulation of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) pathway. …

A Method Of Measuring Hot Charge Carrier Drift Mobility In Germanium Detectors, Kyler Kooi

Dissertations and Theses

High purity germanium is a popular radiation detecting medium due to the excellent energy resolution that is achieved. The unique semiconducting properties of germanium allow a small amount of energy to create free charges in the germanium, which are then collected as a signal. Germanium detectors also have good spatial resolution. The shape of the signal can be used to identify the location of energy deposition in the crystal. Both of these properties make germanium detectors a popular choice in many felds, including security, medical, astrophysics, and particle physics. The properties of a germanium crystal should be well understood and …

The Evolution Of The Iowa Living History Farms (Lhf) From The 1960s Through The 1980s, Ryan Moore

Dissertations and Theses

This master’s thesis examines the emergence and evolution of the Living History Farms (LHF) and the living history farm museum movement in the Midwest region during the decades of the 1960s, 1970s, and 1980s. Originally envisioned as institutions that preserved the knowledge of a form of farming that had largely disappeared due to the rise of post-WWII mechanized commercial agriculture, most Midwestern living history farm museum organizations in the 1970s and 1980s reacted to both societal and museum industry developments and made their exhibit site programming more inclusive of various demographic groups. The LHF responded to this trend by including …

Regional And Landscape Scale Examination And Attribution Of Vegetation Changes To Social-Environmental System Drivers In Kazakhstan, Venkatesh Kolluru

Dissertations and Theses

Kazakhstan (KZ) experienced widespread changes in ecosystem structure and function. The country is a climate and land cover/use change “hotspot” owing to political reforms, intensified grazing, and extreme climatic events. Despite their importance, there is a lack of consensus about vegetation changes, trends, and drivers in KZ. Addressing this knowledge gap is crucial for effectively managing and restoring grassland ecosystems. However, a pressing challenge is discerning anthropogenic-driven vegetation changes from climate variability and decomposing the responses to the complex human-environmental forcings. Motivated by these challenges, I employed statistical and machine learning algorithms to detect and attribute vegetation changes to social-environmental …

8. Facilitating Reflection On Social Robots In Care: Ethics And Pedagogy, Heike Felzmann, Almudena Nava, Miriam Abietar, Esperanza Meri, Karin Christiansen, Mads Lund Andersen, Vivi Søgaard

Irish Journal of Applied Social Studies

No abstract provided.

Development Of A Modular Lab Automation System With Applications To Animal And Bacteria Cell Culture, Timothy William Hartman

Dissertations and Theses

The challenges faced while executing wet lab protocols encourage the development of automation systems to come alongside human scientists. Today’s cutting-edge experiments involve complex protocols with precise measurements usually performed manually. Even simpler biological protocols can be tedious and prone to error, as was seen during the COVID-19 pandemic and society’s demand for high-volume, rapid sample analysis. Moreover, reproducibility suffers when there is excessive variability and insufficient data. Here, we leveraged the Stanford Biodesign process to develop a modular lab automation system and image analysis workflow to address challenges like these. This flagship automation platform at The University of South …

A Systems Informed Approach To The Complex Challenges Of The Industrial Hemp Industry In Pennsylvania, John Ervin

Full-Text Theses & Dissertations

Utilizing systems thinking methodologies and tools, this dissertation explores the complex growth and development challenges faced by the industrial hemp industry in Pennsylvania. Identified were key stakeholders, regulatory barriers, and market forces influencing the industry revealing a misalignment of goals and a lack of stakeholder collaboration as significant impediments. Legal and regulatory frameworks, supply-demand imbalances, and ineffective partnerships emerged as critical factors impacting the industry's future. Recommendations to address misalignments include fostering stakeholder collaboration, improving public-private partnerships, and utilizing interactive planning to address systemic issues to enhance the industry's growth potential.

Chief Blackstar's "Indian Mystery Revue", Michael Claxton Ph.D.

Faculty Research and Publications

No abstract provided.

Osteohistological Assessment Of Edmontosaurus Radii From The Liscomb Bonebed, Prince Creek Formation, North Slope, Alaska, Emma Griffin

Student Research Poster Presentations 2024

Hadrosaurs were one of the most diverse dinosaur groups in the Late Cretaceous period and are known for their numerous articulated skeletons and monodominant bonebeds (Wosik et al., 2020). They are more commonly known as duck-billed dinosaurs and are found globally including latitudinal extremes such as the artic and Antarctica. Edmontosaurus, a genus of hadrosaur, are herbivores and are very large growing up to 8,000 pounds. Edmontosaurus were known to travel in herds as a defense mechanism and were one of the last non-avian dinosaurs living during the late Campanian stage through the end of the Maastrichtian stage, right up …