Digital Commons Network^™

Determination Of Antibiotic Resistance In Salmonella Typhimurium And Salmonella Kentucky Serotypes Of Animal Origin Using Conventional And Molecular Methods, Zeynep Şik, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

The high incidence of multidrug-resistant Salmonella Typhimurium and Salmonella Kentucky isolates is a concern for human and animal health. This study aimed to investigate the antibiotic resistance determinants of a total of 150 S. Typhimurium and S. Kentucky isolates obtained from cows, calves, lambs, and poultry. Salmonella isolates were tested against 13 different antimicrobials using the Kirby-Bauer disc diffusion method, and genotypic antimicrobial resistance determinants were investigated by polymerase chain reaction. Significant differences were detected among serovars for gentamicin, streptomycin, ampicillin, sulfonamide, nalidixic acid, ciprofloxacin, and tetracycline resistance, with the rates of resistance to these antibiotics being determined to be …

Chimeric Antigen Receptor Macrophages Target And Resorb Amyloid Plaques, Alexander B. Kim, Qingli Xiao, Ping Yan, Qiuyun Pan, Gaurav Pandey, Susie Grathwohl, Ernesto Gonzales, Isabella Xu, Yoonho Cho, Hans Haecker, Slava Epelman, Abhinav Diwan, Jin-Moo Lee, Carl J Deselm

2020-Current year OA Pubs

Substantial evidence suggests a role for immunotherapy in treating Alzheimer's disease (AD). While the precise pathophysiology of AD is incompletely understood, clinical trials of antibodies targeting aggregated forms of β amyloid (Aβ) have shown that reducing amyloid plaques can mitigate cognitive decline in patients with early-stage AD. Here, we describe what we believe to be a novel approach to target and degrade amyloid plaques by genetically engineering macrophages to express an Aβ-targeting chimeric antigen receptor (CAR-Ms). When injected intrahippocampally, first-generation CAR-Ms have limited persistence and fail to significantly reduce plaque load, which led us to engineer next-generation CAR-Ms that secrete …

Streamlined Identification Of Clinically And Functionally Relevant Genetic Regulators Of Lower-Tract Urogenital Development, Meade Haller, Yan Yin, Gabe Haller, Tian Li, Qiufang Li, Laura E Lamb, Liang Ma

2020-Current year OA Pubs

Congenital anomalies of the lower genitourinary (LGU) tract are frequently comorbid due to genetically linked developmental pathways, and are among the most common yet most socially stigmatized congenital phenotypes. Genes involved in sexual differentiation are prime candidates for developmental anomalies of multiple LGU organs, but insufficient prospective screening tools have prevented the rapid identification of causative genes. Androgen signaling is among the most influential modulators of LGU development. The present study uses SpDamID technology in vivo to generate a comprehensive map of the pathways actively regulated by the androgen receptor (AR) in the genitalia in the presence of the p300 …

Large-Scale Proteomics Identifies Novel Biomarkers And Circulating Risk Factors For Aortic Stenosis, Khaled Shelbaya, Victoria Arthur, Yimin Yang, Pranav Dorbala, Leo Buckley, Brian Claggett, Hicham Skali, Line Dufresne, Ta-Yu Yang, James C Engert, George Thanassoulis, James Floyd, Thomas R Austin, Anna Bortnick, Jorge Kizer, Renata C C Freitas, Sasha A Singh, Elena Aikawa, Ron C Hoogeveen, Christie Ballantyne, Bing Yu, Josef Coresh, Michael J Blaha, Kunihiro Matsushita, Amil M Shah

Faculty, Staff and Student Publications

Background: Limited data exist regarding risk factors for aortic stenosis (AS). The plasma proteome is a promising phenotype for discovery of novel biomarkers and potentially causative mechanisms.

Objectives: The aim of this study was to discover novel biomarkers with potentially causal associations with AS.

Methods: We measured 4,877 plasma proteins (SomaScan aptamer-affinity assay) among ARIC (Atherosclerosis Risk In Communities) study participants in mid-life (visit 3 [V3]; n = 11,430; age 60 ± 6 years) and in late-life (V5; n = 4,899; age 76 ± 5 years). We identified proteins cross-sectionally associated with aortic valve (AV) peak velocity (AVmax) and dimensionless …

Unraveling The Genetic Architecture Of Congenital Vertebral Malformation With Reference To The Developing Spine, Sen Zhao, Hengqiang Zhao, Lina Zhao, Xi Cheng, Zhifa Zheng, Mengfan Wu, Wen Wen, Shengru Wang, Zixiang Zhou, Haibo Xie, Dengfeng Ruan, Qing Li, Xinquan Liu, Chengzhu Ou, Guozhuang Li, Zhengye Zhao, Guilin Chen, Yuchen Niu, Xiangjie Yin, Yuhong Hu, Xiaochen Zhang, Deciphering Disorders Involving Scoliosis And Comorbidities (Disco) Study, Pengfei Liu, Guixing Qiu, Wanlu Liu, Chengtian Zhao, Zhihong Wu, Jianguo Zhang, Nan Wu

Faculty, Staff and Students Publications

Congenital vertebral malformation, affecting 0.13-0.50 per 1000 live births, has an immense locus heterogeneity and complex genetic architecture. In this study, we analyze exome/genome sequencing data from 873 probands with congenital vertebral malformation and 3794 control individuals. Clinical interpretation identifies Mendelian etiologies in 12.0% of the probands and reveals a muscle-related disease mechanism. Gene-based burden test of ultra-rare variants identifies risk genes with large effect sizes (ITPR2, TBX6, TPO, H6PD, and SEC24B). To further investigate the biological relevance of the genetic association signals, we perform single-nucleus RNAseq on human embryonic spines. The burden test signals are enriched in the notochord …

Metabolic Model Predictions Enable Targeted Microbiome Manipulation Through Precision Prebiotics, Georgios Marinos, Inga K Hamerich, Reena Debray, Nancy Obeng, Carola Petersen, Jan Taubenheim, Johannes Zimmermann, Dana Blackburn, Buck S Samuel, Katja Dierking, Andre Franke, Matthias Laudes, Silvio Waschina, Hinrich Schulenburg, Christoph Kaleta

Faculty, Staff and Students Publications

While numerous health-beneficial interactions between host and microbiota have been identified, there is still a lack of targeted approaches for modulating these interactions. Thus, we here identify precision prebiotics that specifically modulate the abundance of a microbiome member species of interest. In the first step, we show that defining precision prebiotics by compounds that are only taken up by the target species but no other species in a community is usually not possible due to overlapping metabolic niches. Subsequently, we use metabolic modeling to identify precision prebiotics for a two-member Caenorhabditis elegans microbiome community comprising the immune-protective target species Pseudomonas …

Universal Risk Prediction For Individuals With And Without Atherosclerotic Cardiovascular Disease, Yejin Mok, Zeina Dardari, Yingying Sang, Xiao Hu, Michael P Bancks, Lena Mathews, Ron C Hoogeveen, Silvia Koton, Michael J Blaha, Wendy S Post, Christie M Ballantyne, Josef Coresh, Wayne Rosamond, Kunihiro Matsushita

Faculty, Staff and Students Publications

Background: American College of Cardiology/American Heart Association guidelines recommend distinct risk classification systems for primary and secondary cardiovascular disease prevention. However, both systems rely on similar predictors (eg, age and diabetes), indicating the possibility of a universal risk prediction approach for major adverse cardiovascular events (MACEs).

Objectives: The authors examined the performance of predictors in persons with and without atherosclerotic cardiovascular disease (ASCVD) and developed and validated a universal risk prediction model.

Methods: Among 9,138 ARIC (Atherosclerosis Risk In Communities) participants with (n = 609) and without (n = 8,529) ASCVD at baseline (1996-1998), we examined established predictors in the …

Abortion And The Extremism Of Bright Line Rules, Teneille R. Brown

NULR Online

No abstract provided.

Dmhppp1r17 Neurons Regulate Aging And Lifespan In Mice Through Hypothalamic-Adipose Inter-Tissue Communication, Kyohei Tokizane, Cynthia S Brace, Shin-Ichiro Imai

2020-Current year OA Pubs

Recent studies have shown that the hypothalamus functions as a control center of aging in mammals that counteracts age-associated physiological decline through inter-tissue communications. We have identified a key neuronal subpopulation in the dorsomedial hypothalamus (DMH), marked by Ppp1r17 expression (DMH

A Mouse Model To Distinguish Nlrp6-Mediated Inflammasome-Dependent And -Independent Functions, Runzhi Li, Yang Zan, Decai Wang, Xuequn Chen, Anmin Wang, Haoyuan Tan, Guorong Zhang, Siyuan Ding, Chen Shen, Hao Wu, Shu Zhu

2020-Current year OA Pubs

The NOD-like receptor (NLR) family pyrin domain containing 6 (NLRP6) serves as a sensor for microbial dsRNA or lipoteichoic acid (LTA) in intestinal epithelial cells (IECs), and initiating multiple pathways including inflammasome pathway and type I interferon (IFN) pathway, or regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. NLRP6 can exert its function in both inflammasome-dependent and inflammasome-independent manners. However, there is no tool to distinguish the contribution of individual NLRP6-mediated pathway to the physiology and pathology in vivo. Here, we validated that Arg39 and Trp50 residues in the pyrin domain (PYD) of murine NLRP6 are required for …

A Cre-Dependent Reporter Mouse For Quantitative Real-Time Imaging Of Protein Kinase A Activity Dynamics, Elizabeth I Tilden, Aditi Maduskar, Anna Oldenborg, Bernardo L Sabatini, Yao Chen

2020-Current year OA Pubs

Intracellular signaling dynamics play a crucial role in cell function. Protein kinase A (PKA) is a key signaling molecule that has diverse functions, from regulating metabolism and brain activity to guiding development and cancer progression. We previously developed an optical reporter, FLIM-AKAR, that allows for quantitative imaging of PKA activity via fluorescence lifetime imaging microscopy and photometry. However, using viral infection or electroporation for the delivery of FLIM-AKAR is invasive and results in variable expression. Here, we developed a reporter mouse, FL-AK, which expresses FLIM-AKAR in a Cre-dependent manner from the ROSA26 locus. FL-AK provides robust and consistent expression of …

Adaptive Haptic Experience For Existing User Preferences

Defensive Publications Series

A system for providing an adaptive haptic experience is disclosed. The system may associate a first device with a user. The system may also associate a second device with the user. The system may also determine a first haptic drive waveform associated with the first device. The system may also select, from a plurality of second haptic drive waveforms based on the first haptic drive waveform, a second haptic drive waveform.

Proposal Of A Private Caretaker-Completed Questionnaire To Assess The Suitability Of Domestic Cats For Animal-Assisted Interventions, Alina Simona Rusu, Iulia Francesca Pop, Christine Olsen, Magdalena Nawarecka-Piątek, Michal Plewczynski, Eivind Øverli Holm, Dennis C. Turner

People and Animals: The International Journal of Research and Practice

The objective of this study was to develop a caretaker-completed questionnaire entitled “Cats’ Behavioral Suitability for Animal-Assisted Interventions” (CatSAAI), covering two categories of items associated with seven feline personality factors. These feline personality factors were previously identified and validated by Mikkola et al. (2021) on a large sample of domestic cat guardians. The aim of the CatSAAI questionnaire is to facilitate the interpretation of the behavioral suitability or unsuitability of each individual cat for inclusion in feline-assisted interventions, while considering the prevention of stress and risks in AAI settings for both people and animals. The tool was proposed by an …

The Animal Song, Sandy Conoly Mrs.

Lorgnette: Quarterly Review of Children's and Young Adults' Books

No abstract provided.

Rhinos At Recess, Jewell Fine

Lorgnette: Quarterly Review of Children's and Young Adults' Books

No abstract provided.

Our Classroom Rules!, Leah D. Carruth

Lorgnette: Quarterly Review of Children's and Young Adults' Books

No abstract provided.

I Am Temple Grandin, Shan Martinez

Lorgnette: Quarterly Review of Children's and Young Adults' Books

No abstract provided.

I Live In A Tree Trunk, Cynthia Lackey

Lorgnette: Quarterly Review of Children's and Young Adults' Books

No abstract provided.

Destiny Finds Her Way: How A Rescued Baby Sloth Learned To Be Wild, Shan Martinez

Lorgnette: Quarterly Review of Children's and Young Adults' Books

No abstract provided.

All's Right With The World, Leah D. Carruth

Lorgnette: Quarterly Review of Children's and Young Adults' Books

No abstract provided.

Let’S Play God: Commodifying The Human Body, Larkin Carden

Georgia Journal of International & Comparative Law

The global shortage of viable organs for transplantation, exacerbated by the COVID-19 pandemic, highlights a stark contrast between organ donation systems, particularly evident in the United States and Iran. While the United States relies on an altruistic donation system, resulting in millions on its waiting list, Iran's compensation-based approach has nearly eradicated its waitlist. The legal framework of the United States, shaped by property law, historical views on the human body, and federal statutes, complicates its approach to organ donation. The reluctance to grant individual rights to body parts hampers success compared to Iran’s incentivization approach. Beyond learning from Iran’s …

Symposium: One Advocate's 'Junk Science' Is Another Advocate's Evidence: Forging New Paths In Forensic Science

Tennessee Journal of Law and Policy

No abstract provided.

A Short Primer On The Admissibility Of Forensic Science Evidence In Tennessee: A Checklist, Bernard A. Raum

Tennessee Journal of Law and Policy

No abstract provided.

Computational Study Of Binding Of Oseltamivir To Neuraminidase Mutants Of Influenza A Virus, Muhammad Arba, Sri Wahyuli, Setyanto Tri Wahyudi, Amir Karton, Chun Wu

College of Science & Mathematics Departmental Research

Oseltamivir (OTV), which targets the neuraminidase (NA) of Influenza A virus (IAV), has been reported to develop resistance. Here, we performed a computational study on the binding modes of OTV in the wild-type and popular mutants of IAV NA (E119A, E119D, E119G, H274Y, I117T, I117V, I117V-E119A, K150N, N294S, R292K, V116A, and Y252H). The Arg118, Glu119, Asp151, Arg152, Glu276, Arg292, and Arg371 were identified as crucial interacting residues with the drug. The energy decomposition analysis showed that with few exceptions, the dispersion interaction is the dominant interaction, followed by the charge-transfer and polarization interactions. The affinities for OTV were greatly reduced …

Ada News - 02/05/2024, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Faculty Senate Agenda February 5, 2024, Utah State University

Faculty Senate & Faculty Senate Executive Committee

• 3:45 Call to Order
• 3:50 University Business
• 4:05 Faculty Senate Business
• 4:10 Information
• 4:25 Reports
• 4:45 Old Business
• 4:50 New Business
• Adjourn: 5:00 pm

2024 February 05 - Faculty Senate Agenda And Minutes, Faculty Senate, East Tennessee State University

Faculty Senate Agendas and Minutes

No abstract provided.

The Utah Statesman, February 5, 2024, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Taylor Swift-Mania On Social Media Regarding The Big Game, Jin-A Choi, Bond Benton, Yi Luo, Ines Hwang

College of Communication and Media Scholarship and Creative Works

The continuation of America’s most popular sport meeting the world’s most famous pop star seems destined to dominate the airwaves and social media next weekend. Findings from the study included the following. First, in the period when the Chiefs and 49ers advanced to Super Bowl LVIII, Taylor Swift related #superbowl posts on “X” (formerly known as Twitter) totaled more than the COMBINED number of posts about the game’s QB’s and even her beau, Travis Kelce. Second, the social mentions of Taylor Swift and Super Bowl reached two million in the seven-day period (January 28 – February 3, 2024) after Chiefs’ …

Visual Rhetoric Of The Persecuted Christian Right, Jules Carr-Chellman

Father Jack McGrath, S.M., Research Award Presentations

Jules Carr-Chellman, Class of 2024, earned a Father Jack McGrath, S.M., Award for Research in Catholic Intellectual Traditions. This is his presentation of that research at the 2024 Catholic Intellectual Traditions Symposium. Jules completed his project in Prof. Susan Trollinger's ENG 364. Presentation begins at 4:00.