Digital Commons Network^™

Metabolic Potentation Of Antibiotic Lethality From Carbon Source Preference In P. Aeruginosa And S. Aureus, Pooja Parikh, Trent Moudler

Undergraduate Student Symposium

Antimicrobial resistance (AMR) is a growing public health concern with a dire need for new solutions. This problem has only been exacerbated in recent years due to the misuse of antibiotics and globalization. As new antibiotic drugs are costly and time-intensive, it is imperative to understand and study creative new ways to combat AMR. In both natural ecosystems and clinical infections, microorganisms seldom exist in isolation. Rather, they flourish within polymicrobial communities, presenting unique challenges for therapeutic interventions owing to elaborate inter-species interactions. Two such pathogens are Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA); bacteria commonly found in patients with …

Toxoplasma Gondii Seropositivity And Risky Behaviors In College-Age Adults, Roohika Rastogi

Undergraduate Student Symposium

Toxoplasma gondii is a prevalent parasite that has infected more than 40 million people in the USA. House cats and other felids serve as definitive hosts and acquire Toxoplasma by consuming infected prey and intermediate hosts (e.g. rodents). Toxoplasma is known to manipulate prey neurophysiology to enhance its transmission. For example, infected rodents exhibit changes in testosterone, vasopressin, and dopamine. These neurophysiological changes elicit remarkable changes in host behavior: Toxoplasma-infected rodents are more likely to frequent open spaces and are less responsive to normally aversive stimuli (e.g. cat urine). The manipulation of intermediate host neurophysiology and behavior observed in …

Understanding Molecular Packing Using X-Ray Crystallography, Olivia Livingstone

Undergraduate Student Symposium

Foldamers are molecules that fold into predictable three-dimensional shapes. This project seeks to understand the relationship between foldamer shape, hydrogen bonds and the molecular packing mode. This research represents an interdisciplinary collaboration between KAIST, a research university in South Korea, and NSU. We solved the single crystal x-ray structure of a foldamer and expanded the molecular contacts using Mercury to understand how intermolecular hydrogen bonds influence the solid-state packing mode. Each foldamer has a helical shape and makes two N-H^….O hydrogen bonds with molecules on the N-terminus, and also two N-H^….O hydrogen bonds with molecules on …

Utilizing Item-Analyses Method To Identify Opportunities For Improving Mental Health Literacy Training Programs, Aashi Chhabra

Undergraduate Student Symposium

Mental health literacy (MHL) refers to the knowledge, attitudes, and beliefs that are linked to actions in recognizing, managing, and preventing mental health disorders. Acknowledging the importance of quantifying this information, the study aims to explore differences in mental health literacy among various groups. Understanding these disparities is crucial for designing targeted educational campaigns that address specific concerns within the training curriculum.

Participants (n = 170) enrolled in a Mental Health First Aid (MHFA) certification course completed a 15-item pre-survey measuring knowledge and attitudes about mental health. Demographic data was collected, and results were stratified by age, race, gender, and …

A Comparative Analysis Of Serpind1 In A Genomic Lens In Sharks, Vallabh Korivi, Samuel Angel

Undergraduate Student Symposium

This research investigates differences in wound healing between sharks and humans, focusing on key components in the healing pathway. Sharks are known for their remarkable lesion-healing capabilities. Genes such as SERPIND1 play crucial roles in tissue remodeling for sharks while playing a role in cancer metastasis and inflammation in humans. The SERPIND1 gene codes for plasminogen activator inhibitor 1 (PAI-1), which is involved in blood clotting. This clotting process is vital for repairing dermal wounds, sealing off the damaged area, and preventing further blood loss. Comparative genomic analysis reveals distinctions between human and shark SERPIND1 genes at different levels such …

A Comparison Of The Impact Of Colonial Heritage On The Development Of Former Western And Soviet Union Colonies, Sophia Wehle

Undergraduate Student Symposium

How successful were countries colonized by the West in developing compared to countries colonized by the Soviet Union. This paper presented three hypotheses: Former Soviet colonies will be more economically developed than former Western colonies, Former Soviet colonies will be more politically developed than former Western colonies, Former Soviet colonies will be more socially developed than former Western colonies. These hypotheses are derived from the theory that the types of colonialism that the Soviet Union and Western countries practiced were different, meaning that Western European nations created colonies to extract resources from them, rather than to form permanent bonds. This …

A1 Vs A2 Milk Variants And Their Impact On Cysteine Metabolism In Autism-Spectrum Disorder, Esha Patel, Peter Kim

Undergraduate Student Symposium

Dairy cow milk stands as a vital source of nutrients encompassing proteins, carbohydrates, fats, and micronutrients. This nutritional complexity is heightened by the choice between A1 and A2 milk types. This research probes the distinctions between A1 and A2 milk and their potential implications for cysteine metabolism within the context of autism spectrum disorder (ASD). This study focuses on BCM7, a bioactive opioid peptide exclusively released during digestion of A1 milk, and its impact on mitochondrial respiration of macrophage-like THP1 cells.

Using the Seahorse XF for mitochondrial assessment, oxygen consumption rate (OCR), extracellular acidification rate (ECAR), basal respiration, proton leak, …

Assessing Cardiologists' User Experience With Electronic Medical Records And Generative Artificial Intelligence Visualization, Colin Pulickathadam, David Giordano

Undergraduate Student Symposium

Cardiovascular Diseases (CVDs) are a leading cause of death worldwide, accounting for 17.3 million deaths per year. Artificial intelligence has gained prominence in recent years, and there is a significant interest in exploring how artificial intelligence can improve patient outcomes and decrease hospitalization rates. This study will identify the most critical values that cardiologists consider when evaluating patients’ charts, including factors such as medical history, diagnostic results, and current medications. Additionally, the study explores cardiologists' attitudes towards the integration of AI-generated imagery based on these critical values to enhance the efficiency and quality of patient care. A comprehensive survey will …

Assessing Sociodemographic Impact On Mental Health Experience In University Students, Kaitlin Bohorquez

Undergraduate Student Symposium

The COVID-19 pandemic helped shift mental health conversations from taboo to embraced among younger generations. With this shift, young people may be more willing to report on their mental health status, aiding professionals in understanding the relationship between mental health and overall health status. Research supports that factors vary among youth mental health experiences, but gaps in knowledge on the extent sociodemographic factors contribute still exists. This study investigates associations between the mental health experiences (MHE) and sociodemographic factors among college students. This knowledge can inform how to craft mental health programs to suit the needs of diverse university student …

Assessment Of Wearable Vert Technology In Collegeiate Volleyball Athletes, Nicole Stanchfield

Undergraduate Student Symposium

The purpose of this research study is to assess the use of the VERT device with (NCAA Division II Volleyball players. The VERT is a wearable and portable device that measures multiple variables including workload, jump height, frequency, intensity, and impact. Previous research indicates accurate data overall on jump performance which can then be applied to player work loads and injury risk. NCAA Division II Volleyball players included in this study will use the VERT device for one collegiate volleyball season, including practices and games. A typical season includes between 4-5 practices in a week and 1-3 games per week. …

Changes In The Nse And S100b Levels In Plasma Following Treatment With Peripheral Neuropathy-Inducing Chemo Drugs, Shriya Selvakumar, Priya Vontikommu

Undergraduate Student Symposium

Peripheral Neuropathy (PN) is a common chemotherapy-induced side effect that impacts 50% of all patients who receive chemotherapy. Treatment with Bortezomib (BTZ), Cisplatin (CIS), and Vincristine (VIN) has been established as the most common triggers of Chemotherapy-Induced Peripheral Neuropathy (CIPN) among cancer patients. Therefore, it was hypothesized that identifying diagnostic/prognostic biomarkers that can accurately predict the onset or progression of CIPN would help to determine the eligibility of the above-mentioned drugs for chemotherapy use and also would help to proactively prepare the patients for protective care. Therefore, our initial goal was to elucidate the molecular mechanisms underlying the onset and …

Characterizing Visual Literacy In Biochemistry: Interpreting The Symbolic Language Embedded Within Biochemistry Representations, Sahil Sheth, Manav Patel

Undergraduate Student Symposium

Representations play a key role in communicating concepts and phenomena in courses such as chemistry, biology, and biochemistry. While representations have the potential to help students develop conceptual understanding of a subject, integrating multiple ideas to interpret and use the representations can be challenging. For example, biochemistry students are faced with the unique challenge of having to combine their knowledge of both chemistry and biology to make sense of complex biological structures and processes. Using the Visual Literacy Framework proposed by Schönborn and Anderson, the objective of our study is to investigate the symbolism embedded within biochemistry representations and how …

Climate Change Attitudes About Human Responsibility Among The Nsu Community, Anshu Jasani

Undergraduate Student Symposium

This research is about how people self-report climate change attitudes about human responsibility. Self-reports precede actions (Critchfield & Perone, 1990), so a predictive model of attitudes is important. Ransdell (2016) surveyed a similar NSU sample in 2015 and found that 62% reported a belief that climate change is mostly human-caused. The present study is a replication with some additional questions. As before, all with NSU email addresses were sent the survey once, without any incentive or follow-up. In this survey, the main goal was to predict Question 6 which showed participants' views on climate change as mostly human caused. The …

Comparative Genomics Across Ganoderma Species To Identify Anti-Cancer Producing Secondary Metabolite Clusters, Abril Chao, Omobolanle Ayangade, Rajendra Pangeni Ph.D., Braham Dhillon Ph.D.

Undergraduate Student Symposium

Ganoderma lucidum is a mushroom-forming fungus that has been used in traditional Chinese medicine for over 2,000 years. G. lucidum, also known as lingzhi or reishi, is consumed in several Asian cultures to promote health and longevity. Bioactive molecules with anticancer properties have been isolated and characterized primarily from the species Ganoderma lucidum, and the cytotoxic potential of other species in the genus Ganoderma remains relatively unclear. Bioactive molecules isolated from mushroom fruiting bodies are demonstrated to have cytotoxicity against different cancer cell lines. Triterpenes and polysaccharides are the two major groups of compounds that exhibit antitumor and …

Comparative Genomics Across Streptomyces Murinus Strains To Study Conservation Of Biosynthetic Gene Clusters Producing Antifungal Compound Pentamycin, Sheril Mathew, Niah Johnson, Vamsi Korisapati, Dhivyan Kurani, Sathvi Gandra, Rebecca Thomas

Undergraduate Student Symposium

Streptomyces are filamentous, Gram-positive bacteria in the phylum Actinobacteria that are known to produce a diverse array of antimicrobial metabolites. A bacterial isolate, labeled Streptomyces murinus strain SPC1, recovered in Davie, FL, from ripe berries of foxtail palm (Wodyetia bifurcata) exhibited antimicrobial activity against fungal pathogens of palms (Dhillon and Chakrabarti 2023). Interestingly, the genome of Streptomyces strain SPC1 contains a complete secondary metabolite cluster for antifungal metabolite pentamycin (fungichromin) biosynthesis. Secondary metabolite clusters (SMCs) or biosynthetic gene clusters (BGCs) are groups of genes responsible for the production of bioactive compounds, such as antibacterial and antifungal agents. In …

Conserved Domains In Promoters Of Differentially Expressed Genes Under High Night Temperature Stress In Rice: How To Find Them And What Can They Tell Us?, Emalynn Tobias, Jenna Handal, Paul Lup, Michael Egozi

Undergraduate Student Symposium

Crop plant response to stress involves changes in gene expression patterns. The complex process of regulation of gene expression involves cis- and trans-acting components. One of the key steps in understanding changes in gene expression associated with a plant’s response to stress begins with identifying “conserved domains” in the promoters of differentially expressed genes (DEGs). By providing binding sites for transcription factors, the conserved domains can play a crucial role in gene regulation. In this study, we aim to identify the cis-regulatory elements (CREs) in the promoters of 149 DEGs that were identified in the transcriptomic analysis of two rice …

Dehydration And Traumatic Brain Injury, Lauren Gray

Undergraduate Student Symposium

Boxers lose up to 15% of their body weight and on average 6.7% prior to weigh in. Boxers dehydrate to make the weight class and will rapidly rehydrate before fighting. During fights, boxers receive traumatic brain injuries. There is reason to believe that if boxers are not properly rehydrated the traumatic brain injuries could be exacerbated, however, this hypothesis had never been explored. This study aimed to determine the extent of fluid loss and traumatic brain injuries. This study utilized 48 mice. Half of the mice received dehydration followed by rehydration while the other half had normal fluid intake. Half …

Determining The Function Of The Protein Of Interest 2qru Using Bioinformatic Tools And Wet-Lab Experiments, Samhita Doranala, Michelle Milman, Marissa Bonsangue, Eliezer Gurovich

Undergraduate Student Symposium

Proteins are complex macromolecules that play a vital role in the facilitation of biological processes. Protein research and documentation are crucial for future advancements. They aid in developing medications, disease diagnosis and treatment, and the understanding of structural biology. Thousands of proteins have been stored in the Protein Data Bank (PDB) since 1971. Although their structures are known, many of their functions have yet to be determined.

This study was conducted in order to shed light on the biological role of a previously uncharacterized protein of interest (POI) with the PDB ID: 2QRU. A two-pronged approach involving both bioinformatics and …

Determining The Optimal Infection Temperature And Burst Size Of Gordonia Rubripertincta Bacteriophage, Genesis Aguilar, Bianca A. Gonzalez, Sarah Moya, Jose C. Padron, Dani Sheil

Undergraduate Student Symposium

Antibiotic resistance represents a significant global health crisis, especially within hospitals, creating a necessity for the search for alternative therapeutic agents to combat drug-resistant pathogens. One source of treatment is phages specifically targeting G. rubripertincta, a gram-positive, aerobic actinomycete bacterium which has shown intrinsic resistance to some antibiotics. Furthermore, G. rubripertincta can cause endocarditis and CNS infections in immunocompromised individuals. Several phages such as Alyssamiracle, Fribs8, Genamy16, and NovaSharks, have been discovered that successfully target G. rubripertincta. The goal of this research is to determine the optimal infection temperature and the burst size of these phases. By growing …

Developing A Better Animal Model Of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (Me/Cfs), Roberto Fileni, Amanda Lee, Medha Mageswaran

Undergraduate Student Symposium

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a rare but extremely debilitating disease characterized by severe fatigue, even after only mild exertion. In fact, ¼-⅓ of patients are house- or bedridden, underscoring the urgent need for effective treatment. Patients with ME/CFS display hypocortisolism, overactive serotonergic systems, and elevated TGF-β levels, suggesting that ME/CFS includes dysfunction of the endocrine, nervous, and immune systems. Current animal models of ME/CFS use immune activators but lack the complex multisystemic nature of the disease and bear a closer resemblance to depression than ME/CFS. One recent study showed that an adrenalectomy (ADX) better matched the characteristics seen …

Diabetes Progression: From Statistical Analysis To Mathematical Modeling, Sandeep Dronavalli

Undergraduate Student Symposium

Diabetes constitutes one of the leading neuroendocrine conditions with nearly 9.4 % of the US population showing symptoms of it. Noteworthy, type 2 diabetes (T2D) accounts for 90 to 95 % of all cases. It is a chronic disease, and the clinical manifestations include hyperglycemia (prolonged blood glucose levels above normal), hyperinsulinemia (excess levels of insulin circulating in the blood) along with insulin resistance. This presentation includes a statistical analysis based on a cohort of 440 individuals where both, demographic and laboratory measurements are included. Based on these results, a mathematical model accounting for the obtained associations is solved. It …

Distributional Patterns Of Mesopelagic Scyphozoa Within The Monterey Submarine Canyon, California, Diana Phillips, Manuel Ploner

Undergraduate Student Symposium

Scyphozoans are a historically understudied taxonomic class, and it is unknown how genera that potentially compete coexist in resource-limited environments within the Mesopelagic Zone (200-1000m depth). Monitoring the affinities of various genera to fine-scale water mass differences demonstrates how apparent competitors in resource-limited environments occupy different habitats. Remotely Operated Vehicle (ROVs) pinpoint location information and oceanographic conditions of individual specimens, while providing better identification than other survey method options. During a 21-year period (2000 - 2020), images obtained by ROV operators annotated visual captures of scyphozoans, while integrating in situ sensor measurements of conductivity, temperature, depth, and dissolved oxygen (CTDO) …

Effective Use For Design, Aruhi Kodeboyina

Undergraduate Student Symposium

Flyers, which foster human connection and communication, are integral when looking at human interaction throughout various stages of life. They require a balance between attractability and simplicity through both words and visuals, and they can create and/or foster a space for discussion and discourse. In this study, I examine the relationship between texts, visuals, phrasing, and audience in the process of making flyers. Relevant scholarship suggests that the wording/framing on the flier has a large part in whether people are influenced by it. Using my own primary research methods, I also found that participants paid a lot of attention to …

Exploring Alternative Binding Locations Of 5-Ht Antagonist Ketanserin In Microtubules; Implications For Future Research, Caleb Siguenza, Tatum Hedrick

Undergraduate Student Symposium

Recently, it’s been demonstrated that classic psychedelic action is mediated via intracellular mechanisms rather than action at membrane receptors. This intracellular action elicits neuroplastic effect. The conclusion is that these effects are due to activation of intracellular 5-HT2AR based on using ketanserin as a selective antagonist based on its selectivity to bind to the membrane 5-HT2A serotonin receptor. However, neuroplasticity is dependent upon multiple mechanisms and signaling cascades including the dynamics of neuronal microtubules. As the targets of ketanserin’s biological intracellular activity are not fully elucidated, here we investigate the binding potential of ketanserin to domains of the microtubule constituent …

Fish Market, Aria Tan

Undergraduate Student Symposium

Aria Nicolette Tan was born in the United States and then grew up on an island off the coast of Malaysia. After moving back to America, she moved frequently across the US. Influenced by her time in culturally diverse communities, Tan developed a fascination with the simplicity of the human experience. Generation Z's media is heavily centered in personal identity and how it strengthens the connection of an individual to society. This experience serves as an inspiration for Tan's art. Her creative motivation is to represent a journey that is accessible to anyone.

Her painting portfolio titled "Fish Market" contains …

Fluorescence Analysis Of Carboxyphenyl Porphyrins, Janani Prasanna, Ayush Desai, Andersen Cheong, Amanda Leon

Undergraduate Student Symposium

Studies to analyze the effects of metals on the fluorescence properties of porphyrins were carried out by comparing a metalloporphyrin (Cu(II) meso-Tetra(4-carboxyphenyl) porphyrin, abbreviated as Cu–4- CPP) with it freebase counterpart (meso-Tetra(4-carboxyphenyl) porphyrin, abbreviated as 4-CPP) at various emissions. These porphyrins were selected based on their solubility, structure, and electrochemical properties. A trial was carried out in the UV spectrophotometer to obtain the peak wavelengths of major absorption which were then used to carry out the trials in the spectrofluorometer. In each experiment, the resulting fluorescence peaks were obtained at various emission wavelengths. After analyzing the peaks, it was determined …

Fluoride-Mediated Gene Expression Regulated Epigenetically Via H3ac Modification In Ameloblast-Lineage Cells, Melanie Mendonca, Shohei Yamashita, Motoki Okamoto, Susanne Brueckner

Undergraduate Student Symposium

Objectives: Previously we reported that fluoride-mediated histone acetyltransferase (HAT) activation contributed to p53 acetylation to promote fluoride toxicity in mouse ameloblast-like cells (LS8). However, the roles of fluoride-mediated HAT activation in histone acetylation and epigenetic regulation of fluoride-mediated gene expressions remain unidentified. This study demonstrates that fluoride-mediated gene expression is regulated epigenetically via modification of histone acetylation status (H3K27Ac) in transcription start sites (TSS) in LS8 cells.

Methods: LS8 cells were treated with or without 5 mM fluoride for 24 h and subjected to chromatin isolation followed by immunoprecipitation (ChIP) and DNA purification. ChIP assay was performed using antibodies specific …

Genome Characterization And Annotation Of Two Newly Discovered Bacteriophages Makomanhole And Penjamin420, Janelle Bachman Rodríguez, Sara Faruqui, Caleb Siguenza, Taylor R. Butler, Christopher Huang, Brianna Khrakovsky, Kayle Mederos, Monserrat Montanez, Gabriella Nazarov, Ilan Nedjar, Anneliese Rodriguez, Laurent F. Zayas Diaz, Megana S. Guntur, Naitik K. Patel

Undergraduate Student Symposium

While many bacteria are beneficial, some can have negative impacts on humans, animals, and the environment. One avenue of exploration to combat these effects is using bacteriophages (phage). Phage are viruses that target and kill specific bacterial host cells. While approximately 10³¹ bacteriophages exist, 99% of them are undiscovered. In recent years, scientists have utilized phage in real-world applications including fighting antibiotic resistance, food biocontrol, bioremediation, and treating wastewater. Therefore, it is essential to genetically characterize novel bacteriophages for their use in these different bacterial control applications. The aim of this research was to annotate the genomes of newly …

How Do Residents Of South Florida Perceive Aquaculture?, Jayce Oldham

Undergraduate Student Symposium

Aquaculture is the raising of fish either extensively, in cages located in public waterways or coastal waters, or intensively, in closed-circuit tanks on land. Aquaculture is mainly done to produce seafood for human consumption. This study used an IRB-approved survey of South Florida residents to assess attitudes regarding the potential ability of aquaculture to produce seafood and how the process of aquaculture is viewed by the public. The survey used voluntary participants for the main results, with stratified sampling to select participants for a subsequent voluntary focus group. The focus group collected additional information about the views of the participants. …

Identification Of Genes Implicated In Low-Grade Gliomas And The Progression Into Higher Grade Gliomas Through Aberrant Dna Methylation, Mit Patel, Arun Jagarlamudi, Varun Toot

Undergraduate Student Symposium

DNA methylation is a critical epigenetic modification involving the addition of methyl groups to DNA as a regulatory mechanism; aberrant patterns contribute to various diseases. Low-grade gliomas (LGG) arise from aberrant cellular differentiation through altered gene expression of various genes, of which DNA methylation plays a crucial role. LGG’s are characterized as slow growing tumors from glial cells of the central nervous system (CNS). Grade IV gliomas or glioblastomas (GBM) are one of the most aggressive brain tumor types with a poor clinical prognosis and median survival of only 15 months. The purpose of this investigation is to analyze the …