Arid1a Orchestrates Swi/Snf-Mediated Sequential Binding Of Transcription Factors With Arid1a Loss Driving Pre-Memory B Cell Fate And Lymphomagenesis, Darko Barisic, Christopher R Chin, Cem Meydan, Matt Teater, Ioanna Tsialta, Coraline Mlynarczyk, Amy Chadburn, Xuehai Wang, Margot Sarkozy, Min Xia, Sandra E Carson, Santo Raggiri, Sonia Debek, Benedikt Pelzer, Ceyda Durmaz, Qing Deng, Priya Lakra, Martin Rivas, Christian Steidl, David W Scott, Andrew P Weng, Christopher E Mason, Michael R Green, Ari Melnick

Faculty, Staff and Student Publications

ARID1A, a subunit of the canonical BAF nucleosome remodeling complex, is commonly mutated in lymphomas. We show that ARID1A orchestrates B cell fate during the germinal center (GC) response, facilitating cooperative and sequential binding of PU.1 and NF-kB at crucial genes for cytokine and CD40 signaling. The absence of ARID1A tilts GC cell fate toward immature IgM+CD80-PD-L2- memory B cells, known for their potential to re-enter new GCs. When combined with BCL2 oncogene, ARID1A haploinsufficiency hastens the progression of aggressive follicular lymphomas (FLs) in mice. Patients with FL with ARID1A-inactivating mutations preferentially display an immature memory B cell-like state with …

Making Way: A Methods Experiment With Arts-Based Embodied Postformal Autoethnography To Prepare For Research-As-Community Building With A Diverse Urban Elementary School, Kelly Elizabeth Bare

Theses & Dissertations

Using autoethnographic, arts-based, and embodied methods, this postformal dissertation study establishes the first of a two-part research-as-community-building template designed with racially and socioeconomically diverse school communities in mind. Imagined as a double helix in an echo of human DNA, it describes an arts-based, embodied self-study (Helix 1) as a prerequisite for later study of community (Helix 2, not yet complete) typified by mindful engagement across socially constructed lines of difference and attention to the potential building blocks of collaborative leadership across those same lines. As a prerequisite to community work, I explored my lifetime encounters with others of difference (Chang, …

Smarca4 Is A Haploinsufficient B Cell Lymphoma Tumor Suppressor That Fine-Tunes Centrocyte Cell Fate Decisions, Qing Deng, Priya Lakra, Panhong Gou, Haopeng Yang, Cem Meydan, Matthew Teater, Christopher Chin, Wenchao Zhang, Tommy Dinh, Usama Hussein, Xubin Li, Estela Rojas, Weiguang Liu, Patrick K Reville, Atish Kizhakeyil, Darko Barisic, Sydney Parsons, Ashley Wilson, Jared Henderson, Brooks Scull, Channabasavaiah Gurumurthy, Francisco Vega, Amy Chadburn, Branko Cuglievan, Nader Kim El-Mallawany, Carl Allen, Christopher Mason, Ari Melnick, Michael R Green

Faculty, Staff and Student Publications

SMARCA4 encodes one of two mutually exclusive ATPase subunits in the BRG/BRM associated factor (BAF) complex that is recruited by transcription factors (TFs) to drive chromatin accessibility and transcriptional activation. SMARCA4 is among the most recurrently mutated genes in human cancer, including ∼30% of germinal center (GC)-derived Burkitt lymphomas. In mice, GC-specific Smarca4 haploinsufficiency cooperated with MYC over-expression to drive lymphomagenesis. Furthermore, monoallelic Smarca4 deletion drove GC hyperplasia with centroblast polarization via significantly increased rates of centrocyte recycling to the dark zone. Mechanistically, Smarca4 loss reduced the activity of TFs that are activated in centrocytes to drive GC-exit, including SPI1 …

Single-Cell Systems Pharmacology Identifies Development-Driven Drug Response And Combination Therapy In B Cell Acute Lymphoblastic Leukemia, Xin Huang, Yizhen Li, Jingliao Zhang, Lei Yan, Huanbin Zhao, Liang Ding, Sheetal Bhatara, Xu Yang, Satoshi Yoshimura, Wenjian Yang, Seth E Karol, Hiroto Inaba, Charles Mullighan, Mark Litzow, Xiaofan Zhu, Yingchi Zhang, Wendy Stock, Nitin Jain, Elias Jabbour, Steven M Kornblau, Marina Konopleva, Ching-Hon Pui, Elisabeth Paietta, William Evans, Jiyang Yu, Jun J Yang

Faculty, Staff and Student Publications

Leukemia can arise at various stages of the hematopoietic differentiation hierarchy, but the impact of developmental arrest on drug sensitivity is unclear. Applying network-based analyses to single-cell transcriptomes of human B cells, we define genome-wide signaling circuitry for each B cell differentiation stage. Using this reference, we comprehensively map the developmental states of B cell acute lymphoblastic leukemia (B-ALL), revealing its strong correlation with sensitivity to asparaginase, a commonly used chemotherapeutic agent. Single-cell multi-omics analyses of primary B-ALL blasts reveal marked intra-leukemia heterogeneity in asparaginase response: resistance is linked to pre-pro-B-like cells, with sensitivity associated with the pro-B-like population. By …

Sickle Cell Disease In The State Of Tennessee, Ayesha Mukhopadhyay

Electronic Theses and Dissertations Archive

Sickle cell disease (SCD) is an inherited blood disorder. Normal red blood cells are round. In those with SCD, the red blood cells are crescent shaped. In the Unites States (US), more than 90% of those living with SCD are Black. We conducted three epidemiological studies related to the burden of common comorbidities in individuals with SCD, the relationship between SCD and chronic kidney disease, and the effects of historical racial housing policies on births with SCD. First, we evaluated the annual incidence and demographic associations of stroke, seizures, congestive heart failure, asthma, systemic hypertension, and diabetes mellitus in individuals …

First-Time Market Goat, Ashley Longmore, Cheyenne Reid

All Current Publications

This fact sheet is 4-H youth who are thinking about or have recently purchased their first 4-H goat. This fact sheet is a simple outline that provides information on selection, feeding, housing, basic care, and grooming.

First-Time Market Lamb, Ashley Longmore, Cheyenne Reid

All Current Publications

This fact sheet is 4-H youth who are thinking about or have recently purchased their first 4-H lamb. This fact sheet is a simple outline providing more information on selection, feeding, housing, basic care, and grooming.

Arsenic Contamination Needs Serious Attention: An Opinion And Global Scenario, Abhijnan Bhat School Of Food Science And Environmental Health, Kamna Ravi Health, Engineering & Materials Sciences (Hems) Hub, Furong Tian Nanolab Research Centre, Baljit Singh Micra Biodiagnostics Technology Gateway

Articles

Arsenic (As) contamination is a serious global concern, polluting our natural resources, including water and soil, and posing a danger to the environment and public health. Arsenic is present in the groundwater of several countries and this contaminated water is used for irrigation, drinking, and food preparation, which poses the greatest threat to public health. Nearly 106 countries are affected by groundwater arsenic contamination and an estimated 230 million individuals worldwide are exposed to its adverse health effects, including increased cancer risks, associated cardiovascular disease and diabetes, skin lesions, neurological effects, kidney damage, and foetal or cognitive development-related complications. Arsenic …

The Aggregation Effect Of Offshore Mussel Farming On Pelagic Fishes, D Bridger, Mj Attrill, Se Rees, Ev Sheehan

School of Biological and Marine Sciences

The increase in hard artificial structures in the marine environment is contributing to ocean sprawl. As well as adding hard structure to the seabed, mussel aquaculture adds rope droppers and anchor lines to the water column, where such structure would otherwise be absent. This paper aims to monitor the effects of mussel headlines on the pelagic fish community over time, as well as to investigate whether there is an interaction between the age of cultivated mussels with the number of epibiota species and the abundance of pelagic fishes. Two surveys were carried out: a time series monitoring survey to assess …

Indigenizing The Right To A Healthy Environment, Elisabeth Parker, Heather Tanana

Pace Environmental Law Review

The most severe impacts resulting from environmental degradation are experienced by already-vulnerable populations, including Indigenous peoples. A growing number of countries are formally recognizing the basic human right to a healthy environment, which can help realize environmental and climate justice for these communities. On July 28, 2022, the United Nations General Assembly passed a landmark resolution formally recognizing the human right to a clean, healthy, and sustainable environment. The adoption of this resolution represents a pivotal moment in the understanding and implementation of a human rights-based approach to protecting the environment. However, it is important to recognize that historically, Indigenous …

130 Years And Counting Into Forever — New York's Forever Wild Constitutional Amendment And Lessons For Modern Green Amendments, Timothy E. Cox

Pace Environmental Law Review

In the 135 years of New York’s Forever Wild Amendment’s existence, it has been challenged by a range of court cases and thereby interpreted by courts throughout New York. The results of these cases frequently have upheld the heart of Forever Wild: to protect New York’s Forest Preserve land. This Article provides a history of the Forever Wild Amendment, an analysis of the courts’ and New York Attorney General’s interpretations of the Amendment, and a discussion of how this information can guide the future of New York’s Green Amendment.

Human Rights In The News, Joseph Thorpe

Honors Program Theses and Projects

This honors thesis contains thirteen hard news stories that focus on human rights issues in the United States. The United Nations defines Human rights as including, but not limited to, “the right to life and liberty, freedom from slavery and torture, freedom of opinion and expression, the right to work and education, and many more.” Human rights, the U.N. continues, “are essential for making life worth living and are the basis for a just and fair society.”

Improving Pharmacokinetics Of Peptides Using Phage Display, Mallika Asar, Jessica Newton-Northup, Mette Soendergaard

Student Publications

Phage display is a versatile method often used in the discovery of peptides that targets disease-related biomarkers. A major advantage of this technology is the ease and cost efficiency of affinity selection, also known as biopanning, to identify novel peptides. While it is relatively straightforward to identify peptides with optimal binding affinity, the pharmacokinetics of the selected peptides often prove to be suboptimal. Therefore, careful consideration of the experimental conditions, including the choice of using in vitro, in situ, or in vivo affinity selections, is essential in generating peptides with high affinity and specificity that also demonstrate desirable pharmacokinetics. Specifically, …

Meso-Scale Seabed Quantification With Geoacoustic Inversion, Tim Sonnemann, Jan Dettmer, Charles W. Holland, Stan E. Dosso

Electrical and Computer Engineering Faculty Publications and Presentations

Abstract Knowledge of sub-seabed geoacoustic properties, for example depth dependent sound speed and porosity, is of importance for a variety of applications. Here, we present a semi-automated geoacoustic inversion method for autonomous underwater vehicle data that objectively adapts model inference to seabed structure. Through parallelized trans-dimensional Bayesian inference, we infer seabed properties along a 12 km survey track on the scale of about 10 cm and 50 m in the vertical and horizontal, respectively. The inferred seabed properties include sound speed, attenuation, density, and porosity as a function of depth from acoustic reflection coefficient data. Parameter uncertainties are quantified, and …

Molecular Nano-Motors (Mnms): A Novel Delivery Modality For Oligonucleotides, Hamutal Ben Dov, Hagit Grimberg

Delivery Of Nucleic Acid Therapeutics II: Biology, Engineering And Development

Please click Additional Files below to see the full abstract.

Dream-Seekers (Ladson-Billings, 2009): An Analysis Of Culturally Relevant Pedagogical Development Through The Perspective Of Black Novice Teachers, Christina Grove, Nana Becoat, Michelle Cooley, Pablo Ramos Jr

Dissertations

ABSTRACT

Using critical race theory and Gloria Ladson-Billings' culturally relevant pedagogy as theoretical frameworks, this thematic narrative analysis study explored the epistemology and ontology of culturally relevant pedagogy by investigating the research question: How do Black novice teachers (years 1-5) become culturally relevant practitioners? The researchers conducted semi-structured interviews with Black K-12 public school teachers in which the participants reflected on how their upbringing, cultural identities, K-12 experiences, and teacher preparation program/process impacts their readiness to practice culturally relevant pedagogy. Five themes emerged from the narrative analysis: 1) K-12 racialized trauma; 2) racialized awakening and awareness; 3) the sanctuary of …

“I Saw Guns And Sharp Swords In The Hands Of Young Children”: Why Mental Health Courts For Juveniles With Autism Spectrum Disorder And Fetal Alcohol Spectrum/Disorder Are Needed, Michael Perlin, Heather Cucolo, Deborah Dorfman

Northwestern Journal of Law & Social Policy

In this Article, we offer—we believe for the first time in the scholarly literature—a potentially (at least partially) ameliorative solution to the problems faced by persons with autism (ASD) and fetal alcohol disorder (FASD) in the criminal justice system: the creation of (separate sets of) problem-solving juvenile mental health courts specifically to deal with cases of juveniles in the criminal justice system with ASD, and with FASD. There is currently at least one juvenile mental health court that explicitly accepts juveniles with autism, but there are, to the best of our knowledge, no courts set up specifically for these two …

Gut Microbial Dysbiosis Differs In Two Distinct Cachectic Tumor-Bearing Models Consuming The Same Diet, Lauri O. Byerley, Brittany Lorenzen, Hsiao Man Chang, William G. Hartman, Michael J. Keenan, Ryan Page, Meng Luo, Scot E. Dowd, Christopher M. Taylor

Faculty Publications

The impact of cancer cachexia on the colonic microbiota is poorly characterized. This study assessed the effect of two cachectic-producing tumor types on the gut microbiota to determine if a similar dysbiosis could be found. In addition, it was determined if a diet containing an immunonutrient-rich food (walnuts) known to promote the growth of probiotic bacteria in the colon could alter the dysbiosis and slow cachexia. Male Fisher 344 rats were randomly assigned to a semi-purified diet with or without walnuts. Then, within each diet group, rats were further assigned randomly to a treatment group: tumor-bearing ad libitum fed (TB), …

Gut Microbial Dysbiosis Differs In Two Distinct Cachectic Tumor-Bearing Models Consuming The Same Diet, Lauri O. Byerley, Brittany Lorenzen, Hsiao Man Chang, William G. Hartman, Michael J. Keenan, Ryan Page, Meng Luo, Scot E. Dowd, Christopher M. Taylor

School of Medicine Faculty Publications

The impact of cancer cachexia on the colonic microbiota is poorly characterized. This study assessed the effect of two cachectic-producing tumor types on the gut microbiota to determine if a similar dysbiosis could be found. In addition, it was determined if a diet containing an immunonutrient-rich food (walnuts) known to promote the growth of probiotic bacteria in the colon could alter the dysbiosis and slow cachexia. Male Fisher 344 rats were randomly assigned to a semi-purified diet with or without walnuts. Then, within each diet group, rats were further assigned randomly to a treatment group: tumor-bearing ad libitum fed (TB), …

Timing Along The Cardiac Cycle Modulates Neural Signals Of Reward-Based Learning, Elsa F. Fouragnan, Billy Hosking, Yin Cheung, Brooke Prakash, Matthew Rushworth, Alejandra Sel

School of Psychology

Natural fluctuations in cardiac activity modulate brain activity associated with sensory stimuli, as well as perceptual decisions about low magnitude, near-threshold stimuli. However, little is known about the relationship between fluctuations in heart activity and other internal representations. Here we investigate whether the cardiac cycle relates to learning-related internal representations – absolute and signed prediction errors. We combined machine learning techniques with electroencephalography with both simple, direct indices of task performance and computational model-derived indices of learning. Our results demonstrate that just as people are more sensitive to low magnitude, near-threshold sensory stimuli in certain cardiac phases, so are they …

A Gnotobiotic Mouse Model With Divergent Equol-Producing Phenotypes: Potential For Determining Microbial-Driven Health Impacts Of Soy Isoflavone Daidzein, Lindsay M. Leonard, Abigayle M.R. Simpson, Shiyu Li, Lavanya Reddivari, Tzu-Wen L. Cross

Purdue University Libraries Open Access Publishing Fund

The implications of soy consumption on human health have been a subject of debate, largely due to the mixed evidence regarding its benefits and potential risks. The variability in responses to soy has been partly attributed to differences in the metabolism of soy isoflavones, compounds with structural similarities to estrogen. Approximately one-third of humans possess gut bacteria capable of converting soy isoflavone daidzein into equol, a metabolite produced exclusively by gut microbiota with significant estrogenic potency. In contrast, lab-raised rodents are efficient equol producers, except for those raised germ-free. This discrepancy raises concerns about the applicability of traditional rodent models …

Pparα Is One Of The Key Targets For Dendrobine To Improve Hepatic Steatosis In Nafld., Yanzhe Xu, Miao Wang, Yi Luo, Hao Liu, Hua Ling, Yuqi He, Yanliu Lu

PCOM Scholarly Works

ETHNOPHARMACOLOGICAL RELEVANCE: Dendrobium nobile Lindl. (DNL) is a traditional Chinese ethnobotanical herb. Dendrobine (DNE) has been designated as a quality indicator for DNL in the Chinese Pharmacopoeia. DNE exhibits various pharmacological activities, including the reduction of blood lipids, regulation of blood sugar levels, as well as anti-inflammatory and antioxidant properties.

AIM OF THE STUDY: The objective of this study is to explore the impact of DNE on lipid degeneration in nonalcoholic fatty liver disease (NAFLD) liver cells and elucidate its specific mechanism. The findings aim to offer theoretical support for the development of drugs related to DNL.

MATERIALS AND METHODS: …

The Evolving Role Of Monomethyl Fumarate Treatment As Pharmacotherapy For Relapsing-Remitting Multiple Sclerosis, Alan D. Kaye, John Lacey, Viet Le, Ahmed Fazal, Nicole A. Boggio, Dorothy H. Askins, Lillian Anderson, Christopher L. Robinson, Antonella Paladini, Chizoba N. Mosieri, Adam M. Kaye, Shahab Ahmadzadeh, Sahar Shekoohi, Giustino Varrassi

School of Medicine Faculty Publications

Multiple sclerosis is the most common autoimmune disease affecting the central nervous system (CNS) worldwide. Multiple sclerosis involves inflammatory demyelination of nerve fibers in the CNS, often presenting with recurrent episodes of focal sensory or motor deficits associated with the region of the CNS affected. The prevalence of this disease has increased rapidly over the last decade. Despite the approval of many new pharmaceutical therapies in the past 20 years, there remains a growing need for alternative therapies to manage the course of this disease. Treatments are separated into two main categories: management of acute flare versus long-term prevention of …

Enhanced Plant-Derived Vesicles For Nucleotide Delivery For Cancer Therapy, Sara Corvigno, Yuan Liu, Emine Bayraktar, Elaine Stur, Nazende Nur Bayram, Adrian Lankenau Ahumada, Supriya Nagaraju, Cristian Rodriguez-Aguayo, Hu Chen, Thanh Chung Vu, Yunfei Wen, Han Liang, Li Zhao, Sanghoon Lee, Gabriel Lopez-Berestein, Anil K Sood

Faculty, Staff and Student Publications

Small RNAs (microRNAs [miRNAs] or small interfering RNAs [siRNAs]) are effective tools for cancer therapy, but many of the existing carriers for their delivery are limited by low bioavailability, insufficient loading, impaired transport across biological barriers, and low delivery into the tumor microenvironment. Extracellular vesicle (EV)-based communication in mammalian and plant systems is important for many physiological and pathological processes, and EVs show promise as carriers for RNA interference molecules. However, some fundamental issues limit their use, such as insufficient cargo loading and low potential for scaling production. Plant-derived vesicles (PDVs) are membrane-coated vesicles released in the apoplastic fluid of …

"Moloch Of The Present Mode": Women's Short Hairstyles In Nineteenth-Century American Society, Jack Tingey

The Thetean: A Student Journal for Scholarly Historical Writing

An article from Indianapolis journal in 1883 reported on the phenomenon of women cutting their hair short in the name of fashion, describing the process of a woman cutting off her long hair as akin to sacrificing virtue to the "Moloch of the present mode." Today, short hairstyles are more commonly associated with the bob of the Roaring Twenties, an era historians and popular culture recognize as one of excess, social change, and new innovations. But short hair on women was by no ~ans new in the 1920s. The bob was not the first short hairstyle in the United States …

Maya-Catholic Theologian: The Influence Of Maya Theology On Catholic Doctrine In The Morley Manuscript, Travis Meyer

The Thetean: A Student Journal for Scholarly Historical Writing

In 1576, at the height of Catholic evangelization in the Yucatan Peninsula in Mexico, an unnamed Catholic preacher met regularly with a congregation of indigenous Maya to teach them the doctrine of Christianity. He taught them about the biblical creation, as well as the nature of God and the devil, all from his own original doctrinal instructional manual. This preacher was also a Maya himself Between the years 1500 and 1800, only about 1,100 European-descended Catholic friars ministered to the tens of millions of Maya in Mesoamerica. Because there were so many natives and so few friars, they could not …

Healing A Generation; Implementation Of Higher Education Curricula For Venezuelan Journalism Students Living Under Structural Violence To Promote A Transition Into Democracy, José Luis Jiménez-Figarotti Prof.

The SUNY Journal of the Scholarship of Engagement: JoSE

Venezuela's sociopolitical landscape has deteriorated significantly over the past decade, culminating in a profound humanitarian crisis. This ethnography, conducted from 2015 to the present, explores the experiences of a study group comprising 2000 Venezuelan communication college students, aged 17 to 25, who navigate structural violence while striving for quality higher education. The research employed a multifaceted approach, encompassing interviews, focus groups, and observations. Additionally, this qualitative study examines the outcomes of implementing an interdisciplinary journalism curriculum grounded in human rights and media activism, complemented by online sessions and an environmental education component. This educational project aims to foster critical thinking …

Water Use Efficiency In Chilean And Argentine Humid Temperate Grass-Legume Pastures, H G. Landi, M H. Wade, V Garcia De Cortazar, D E. Dalla Valle

IGC Proceedings (1977-2023)

At two sites in Argentina and Chile five levels of water input were applied to four sown pastures of varying ages during spring and summer. The pastures consisted principally of C3 grasses and legumes, some of which were sown such as Lolium perenne, Trifolium repens, Dactylis glomerata. Dry matter (DM) production was measured and related to the estimated total evapotranspiration (ET): responses were both highly linear. Both responses to ET and absolute yields were higher at the Argentinian than at the Chilean site: respectively 10.7 and 15.2 kg DM/mm water evapotranspired. Nevertheless the calculated indices of sensitivity (Ky) of Doorenbos …

Leaf Anatomy And Ultrastructure Of Poa Ligularis After Defoliation And Water Stress, M G. Klich, R E. Brevedan, S C. Villamil

IGC Proceedings (1977-2023)

The objective of this study was to determine the effect of defoliation and water stress on leaf anatomy and the mesophyll cell ultrastructure of Poa ligularis. Anatomical differences were detected mainly at epidermal level. Under water stress, leaf roughness was enhanced since the epidermal-cell surface contours became conspicuous. Under defoliation the microscopic roughness decreased as a result of fewer epicuticular wax crystals being formed. Defoliation produced an enhancement of the interlamellar spaces in the irrigated plantís chloroplast. Under no defoliation conditions, chloroplast structure was not affected by water stress. When subjected to water stress, lightly defoliated plants presented chloroplasts …

Leaf Growth And Anatomy During Winter Droughting Of Tetrachne Dregei Plants, M G. Klich, R E. Brevedan, H Laborde

IGC Proceedings (1977-2023)

The purpose of this work was to study the leaf growth and anatomy of Tetrachne dregei plants when they were subjected to a winter drought period. Plants were grown in semicontrolled conditions under two soil moisture regimes: irrigated and non-irrigated. Anatomical characterization was made using optical and scanning electron microscopy. No differences were found in stomatal index, stomatal length and epidermal cell size, between irrigated and non-irrigated plants. The adaxial epidermis of water stressed plants was more pubescent. Leaf abaxial epidermis of non-irrigated plants showed more intercostal plates of crystallized epicuticular wax than that of the irrigated ones. Leaf tissue …