Digital Commons Network^™

Creating A Bio-Based Circular Economy From Louisiana Sugarcane Byproducts, G. Aita, D. Bhatnagar, G. O. Bruni, M. Deliberto, G. Eggleston, A. Finger, K. Gravois, M. Isied, W. Judice, K. T. Kasson, I. M. Lima, J. L. Purswell, Mena I. Souliman, E. Terrell, B. S. Tubana, H. L. Waguespack Jr., J. J. Wang, P. M. White Jr.

Civil Engineering Faculty Publications and Presentations

Sugarcane (Saccharum officinarum) is Louisiana's number one row crop. Growing and processing sugarcane produces significant amounts of byproducts, including bagasse, crop residue, molasses, filter-press mud, and boiler fly ash. These products represent an important opportunity to generate value-added and specialty products and enhance sugarcane's sustainability by facilitating a circular economy, where agricultural by-products are reused instead of disposing them (linear economy), in order to reduce resource use and energy demand. Examples of value-added products range from biochar, construction materials, animal feed, biofuels, nanoparticles, and fertilizer. Paramount to the success of the bio-based circular economy is creating useful products that are …

Rnai “Flexon” Approach To Gonad Gene Knockdown In C. Elegans, Sofia Suzanne Douglas

Undergraduate Theses

All living things require some form of genetic regulation so that gene products are correctly produced and maintained. One significant form of post-transcriptional gene expression is RNA silencing, a biological mechanism in which double stranded RNA molecules inhibit gene expression by blocking translation of targeted mRNA molecules. Due to its extensive applications in biotechnology, it’s been adapted as an experimental technique referred to as RNAi. This technique is temporary, transient, and easy to use. A new form of RNAi, referred to as the “Flexon” technique, is a novel approach that provides a method for post-transcriptional gene regulation that allows for …

The Impact Of Aging On Fibroblast Gene Expression In Post-Extraction Wound Healing: A Single-Cell Rna Sequencing Study, Paula Christina Cohen Schlemmer

Theses & Dissertations

Wound healing is slower in aged patients. Immediately after tooth extraction, fibroblasts are activated to rebuild the ECM, which is later remodeled through the expression of MMPs. Gene expression may be altered in fibroblasts of aged patients leading to deficiencies in ECM deposition and the recruitment and proliferation of immune cells, resulting in a slow-healing fibrotic wound.

Using scRNA-seq, soft tissue was profiled from two healthy patients: one young and one aged at the time of tooth extraction. Two weeks post-extraction, a second soft tissue sample was harvested. Differentially expressed genes were used to identify enriched pathways for each …

Interspecific Venom Variation Among Three Shades Of Aphonopelma Tarantulas: A. Gabeli, A. Hentzi, And A. Anax, Jorjia Elmore

Honors Theses

Phenotypic variation is a common occurrence throughout the animal kingdom and can occur both interspecifically and intraspecifically. Interspecific variation evolves in response to differential selection between species whereas intraspecific variation is commonly expressed as sex-based or population-based differences. Both interspecific and intraspecific phenotypic differences occur frequently in venomous species including arachnids, and can often result in differences in venom yield, potency, and protein. The North American tarantulas (Theraphosidae: Aphonopelma), include approximately 35 species and little is known about their venom. To test for interspecific and intraspecific venom variation, we collected individuals from three species and generated proteomic profiles and Illumina …

Serum Antibodies To Periodontal Pathogens And Anti-Malondialdehyde-Acetaldehyde: Potential Role In The Interrelationship Between The Periodontium And Rheumatoid Arthritis, Joyce Lee

Theses & Dissertations

Objectives: Study 1 – To quantify associations between anti-Porphyromonas gingivalis, anti-Prevotella intermedia, and anti-Fusobacterium nucleatum serum antibody concentrations and risk of developing rheumatoid arthritis (RA). Study 2 – To test if periodontal clinical measures, alveolar bone loss (ABL), and the aforementioned serum anti-bacterial antibody concentrations are associated with anti-malondialdehyde-acetaldehyde (MAA) serum antibody concentrations.

Methods: Study 1 – Serum samples pre- and post- RA diagnosis (n=214 cases, 210 matched controls) from the U.S. Department of Defense Serum Repository were utilized to compare timing of elevations of anti-bacterial antibody concentrations relative to RA diagnosis and associations …

Cytotoxic Sigma-2 Ligands Trigger Cancer Cell Death Via Cholesterol-Induced-Er-Stress, Rony Takchi, Bethany C Prudner, Qingqing Gong, Takaomi Hagi, Kenneth F Newcomer, Linda X Jin, Suwanna Vangveravong, Brian A Van Tine, William G Hawkins, Dirk Spitzer

2020-Current year OA Pubs

Sigma-2-ligands (S2L) are characterized by high binding affinities to their cognate sigma-2 receptor, overexpressed in rapidly proliferating tumor cells. As such, S2L were developed as imaging probes (ISO1) or as cancer therapeutics, alone (SV119 [C6], SW43 [C10]) and as delivery vehicles for cytotoxic drug cargoes (C6-Erastin, C10-SMAC). However, the exact mechanism of S2L-induced cytotoxicity remains to be fully elucidated. A series of high-affinity S2L were evaluated regarding their cytotoxicity profiles across cancer cell lines. While C6 and C10 displayed distinct cytotoxicities, C0 and ISO1 were essentially non-toxic. Confocal microscopy and lipidomics analysis in cellular and mouse models revealed that C10 …

Growth From Within, Raevenne Zackery

Graduate Artistry Projects and Performances

My intersection of art and biology and the body of work that I created transcends who I am as a person not only an artist, but my appreciation in the love for science. Specifically, nature impacts the entire world. The simplicity of thinking about nature is the cycle of life I found myself, beginning a career as a biology major, wanting to do biomedical research with life developing vitamins and skin care, but ideally, I wanted to be different and every possible way the genre of display nature or the impact of an environment shows more complexity to the way …

I'M Not A Robot, Dean Krueger

Graduate Artistry Projects and Performances

My dystopian short film "The Ballad of Viktor GRC-01" seeks to tap into the essence of animation as a humanist art form, a distorted mirror to reality rendered in pencil. The mark making and compositions more closely resemble German expressionist woodcuts, or early 20^th century comic strips with stark contrasting monochromatic ink blotches and screen tones.

Character dynamics are implied through staging and body language as opposed to dialogue, and based off the assumption of the audience being willing to fill in their own gaps of meaning in the ambiguity, not dissimilar to a receptive audience to abstract modern …

Maine Mill Digital Curation, Catherine E. Kiburis, Amanda Ford, Pat Pasong, Isabelle Zoerb, Lindsay Fortin, Sophia Schlichtmann, Brady Rice, Averie M. Argir, Bella L. Demarco

The Hugemanities Project Big Contest

We worked with the Maine Mill Museum to help document the community response to the Lewiston shootings. We collected information and media for the museum workers to memorialize the victims, their families, and the events.

Fiddler Crab Gut Microbiomes As An Indicator For Eutrophication Due To Waste-Water Contamination In Estuaries, Madison L. Geraci

Honors Theses

Estuaries are unique biogeochemical zones in coastal systems that provide a myriad of ecological functions: providing habitat for critically important fishery species, diverse species of plants and animals, and contributing to nutrient cycling. Estuaries are vulnerable to anthropogenic stressors as they are intersections between marine and terrestrial communities and are often the hotspot of coastal development and activity. Estuarine sediments act as repositories for anthropogenic contaminants, including excess nutrient runoffs and waste-water pollution that can cause states of eutrophication. Microorganisms are sensitive to these environmental changes, presenting a unique opportunity of being useful bioindicators of environmental stress. Isolating microorganisms from …

Cytauxzoonosis In Indiana, Usa: A Case Series Of Cats Infected With Cytauxzoon Felis (2018-2022), Mason V. Reichard, Stacy R. Cotey, Sriveny Dangoudoubiyam, Pabasara Weerarathne, Kenneth Tussey, Rebecca P. Wilkes, Et. Al.

Michigan Tech Publications

CASE SERIES SUMMARY: This case series describes six cases involving seven cats naturally infected with Cytauxzoon felis in Indiana, USA. Medical records were retrospectively reviewed and all available information on signalment, history, clinical and diagnostic findings, treatment, outcome and pathology was reported. Cats infected with C felis were domestic shorthairs, were aged between 2 and 9 years and all but one of the cats were male. The seven infected cats originated from five counties in southwestern Indiana. Six of seven cats were found to have acute cytauxzoonosis based on clinical signs, gross pathologic lesions, observation of C felis in tissues …

Bitterness And Anger In Ephesians, Archetypes, & The Bi-Hemispheric Structure Of The Brain: Comparing Paul, Jordan Peterson, And Iain Mcgilchrist, Joshua Armstrong

Grapho : Concordia Seminary Student Journal

Many people eager to confront falsehood and pursue justice or truth do so nourishing a vindictive, bitter, or resentful attitude. Nourishing anger, particularly resentment, is akin to stoking up the archetypal “Luciferian spirit,” according to clinical psychologist and author Jordan Peterson. This spirit presumes: “what I do is all there is to do, what I know is all there is to know.” It is symptomatic of attending to the world in a way overly reliant on a “left-hemisphere” approach, which leads to entrapment in a “self-reflexive virtual world” disconnected from real “other” things, and only really knowing itself, according to …

Grapho 2024, Cody Macmillan

Grapho : Concordia Seminary Student Journal

Grapho 2024

The Effect Of Diabetes Mellitus On Differentiation Of Mesenchymal Stem Cells Into Insulin-Producing Cells, Mohamed M. Kamal, Omar I. Badr, Shohda A. El-Maraghy, Heba R. Ghaiad

Pharmacy

Background

Diabetes mellitus (DM) is a global epidemic with increasing incidences. DM is a metabolic disease associated with chronic hyperglycemia. Aside from conventional treatments, there is no clinically approved cure for DM up till now. Differentiating mesenchymal stem cells (MSCs) into insulin-producing cells (IPCs) is a promising approach for curing DM. Our study was conducted to investigate the effect of DM on MSCs differentiation into IPCs in vivo and in vitro.

Methods

We isolated adipose-derived mesenchymal stem cells (Ad-MSCs) from the epididymal fat of normal and STZ-induced diabetic Sprague–Dawley male rats. Afterwards, the in vitro differentiation of normal-Ad-MSCs (N-Ad-MSCs) and …

The Cowl - V. 88 N. 8 - May 2, 2024

The Cowl

The Cowl - student newspaper of Providence College. Vol. 88 No. 8 - May 2, 2024. 28 pages.

Identification Of Compounds That Cause Axonal Dieback Without Cytotoxicity In Dorsal Root Ganglia Explants And Intervertebral Disc Cells With Potential To Treat Pain Via Denervation, Fei San Lee, Uyen N. Nguyen, Eliza J. Munns, Rebecca A. Wachs

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Low back pain, knee osteoarthritis, and cancer patients suffer from chronic pain. Aberrant nerve growth into intervertebral disc, knee, and tumors, are common pathologies that lead to these chronic pain conditions. Axonal dieback induced by capsaicin (Caps) denervation has been FDA-approved to treat painful neuropathies and knee osteoarthritis but with shortterm efficacy and discomfort. Herein, we propose to evaluate pyridoxine (Pyr), vincristine sulfate (Vcr) and ionomycin (Imy) as axonal dieback compounds for denervation with potential to alleviate pain. Previous literature suggests Pyr, Vcr, and Imy can cause undesired axonal degeneration, but no previous work has evaluated axonal dieback and cytotoxicity …

Kenyon Collegian - May 2, 2024

The Kenyon Collegian

No abstract provided.

Fam86a Methylation Of Eef2 Links Mrna Translation Elongation To Tumorigenesis, Joel William Francis, Simone Hausmann, Sabeen Ikram, Kunlun Yin, Robert Mealey-Farr, Natasha Mahealani Flores, Annie Truc Trinh, Tourkian Chasan, Julia Thompson, Pawel Karol Mazur, Or Gozani

Faculty, Staff and Student Publications

eEF2 post-translational modifications (PTMs) can profoundly affect mRNA translation dynamics. However, the physiologic function of eEF2K525 trimethylation (eEF2K525me3), a PTM catalyzed by the enzyme FAM86A, is unknown. Here, we find that FAM86A methylation of eEF2 regulates nascent elongation to promote protein synthesis and lung adenocarcinoma (LUAD) pathogenesis. The principal physiologic substrate of FAM86A is eEF2, with K525me3 modeled to facilitate productive eEF2-ribosome engagement during translocation. FAM86A depletion in LUAD cells causes 80S monosome accumulation and mRNA translation inhibition. FAM86A is overexpressed in LUAD and eEF2K525me3 levels increase through advancing LUAD disease stages. FAM86A knockdown attenuates LUAD cell proliferation and suppression …

Factors Affecting Implementation Of Interventions For Oral Health, Substance Use, Smoking And Diet For People With Severe And Multiple Disadvantage: A Community-Based Qualitative Study In England, Neha Jain, Emma A Adams, Emma C Joyes, Gillian Mclellan, Martin Burrows, Martha Paisi, Laura J Mcgowan, Lorenzo Iafrate, David Landes, Richard G Watt, Falko F Sniehotta, Eileen Kaner, Sheena E Ramsay

Peninsula Dental School

Background Populations facing severe and multiple disadvantage (SMD), co-occurring homelessness, substance use and repeat offending have high levels of physical and mental ill-health. Poor oral health is one of the most common health problems in this population and is closely linked with substance use, smoking and poor diet. Issues related to the implementation of interventions among SMD populations to address oral health and related health behaviours are poorly understood. This study aimed to understand the factors that affect implementation (relevance of setting, acceptability and adverse effects of interventions) and the sustainability of interventions targeting oral health, substance use, smoking and …

Spartan Daily, May 2, 2024, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2024

Volume 162, Issue 40

Community-Acquired Pneumonia: A Us Perspective On The Guideline Gap, Maricar Malinis, Lilian Abbo, Jose A Vazquez, Luis Ostrosky-Zeichner

Faculty, Staff and Student Publications

Community-acquired pneumonia continues to be one of the most common causes of morbidity and mortality due to infectious disease. The aetiologies, clinical presentations, diagnostic modalities and therapeutic options are changing and outpacing the creation of management guidelines. This educational article summarizes a roundtable activity sponsored by an unrestricted educational grant by Paratek that included US experts discussing these changes and identifying gaps in the current guidelines.

Mdm2 Inhibitors For Cancer Therapy: The Past, Present, And Future, Wei Wang, Najah Albadari, Yi Du, Josef F Fowler, Hannah T Sang, Wa Xian, Frank Mckeon, Wei Li, Jia Zhou, Ruiwen Zhang

Faculty, Staff and Student Publications

Since its discovery over 35 years ago, MDM2 has emerged as an attractive target for the development of cancer therapy. MDM2's activities extend from carcinogenesis to immunity to the response to various cancer therapies. Since the report of the first MDM2 inhibitor more than 30 years ago, various approaches to inhibit MDM2 have been attempted, with hundreds of small-molecule inhibitors evaluated in preclinical studies and numerous molecules tested in clinical trials. Although many MDM2 inhibitors and degraders have been evaluated in clinical trials, there is currently no Food and Drug Administration (FDA)-approved MDM2 inhibitor on the market. Nevertheless, there are …

May 2, 2024, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

De Novo Genome Assembly For The Coppery Titi Monkey (Plecturocebus Cupreus): An Emerging Nonhuman Primate Model For Behavioral Research, Susanne P Pfeifer, Alexander Baxter, Logan E Savidge, Fritz J Sedlazeck, Karen L Bales

Faculty, Staff and Students Publications

The coppery titi monkey (Plecturocebus cupreus) is an emerging nonhuman primate model system for behavioral and neurobiological research. At the same time, the almost entire absence of genomic resources for the species has hampered insights into the genetic underpinnings of the phenotypic traits of interest. To facilitate future genotype-to-phenotype studies, we here present a high-quality, fully annotated de novo genome assembly for the species with chromosome-length scaffolds spanning the autosomes and chromosome X (scaffold N50 = 130.8 Mb), constructed using data obtained from several orthologous short- and long-read sequencing and scaffolding techniques. With a base-level accuracy of ∼99.99% in chromosome-length …

Vista: May 2, 2024, University Of San Diego

USD Vista (1965-)

No abstract provided.

Traditional Dowry In Repressing Papuan Women’S Political Representation Jayapura City Legislative 2014-2019, Ferinandus Leonardo Snanfi

Jurnal Wacana Politik

This research investigated the impact of traditional dowry payments on the low political representation of indigenous Papuan women in the Jayapura City legislature from 2014 to 2019. Despite the election of four indigenous Papuan women to the Jayapura City legislature in 2014, there remains a significant gap in representation. This qualitative research gathered data through interviews with 30 respondents, including seven influential community figures, seven husbands of indigenous Papuan women, six Papuan students, two lecturers, three members of political parties, two female Papuan legislators, one representative from the General Election Commission, one member of the Papuan People’s Council, and the …

Lessons Learned From Protein Kinase C Epsilon Peptide Mitigation Of Ischemia/Reperfusion Injury For Post-Transplant Acute Kidney Injury (Aki) And The Key Function Of Uncoupled Endothelial Nitric Oxide Synthase In This Process, Taurai Augustin, Kerry-Anne Perkins, Desmond Tanoh Boakye, Yanlin Jiang, James George, Anupam Agarwal, Lindon H. Young

Research Day

Introduction

Ischemia-reperfusion (I/R) injury is marked by increased reactive oxygen species (ROS) during reperfusion, causing cellular injury. Uncoupled endothelial nitric oxide synthase (eNOS) is a major source of ROS in myocardial I/R, hindlimb I/R, and kidney extracorporeal shock wave lithotripsy. During reperfusion, activation of protein kinase C epsilon (PKCε) enhances ROS via uncoupled eNOS due to an increased dihydrobiopterin/tetrahydrobiopterin ratio. Inhibiting uncoupled eNOS using myristic acid conjugated-PKCε peptide inhibitor (N-Myr-EAVSLKPT [Myr-PKCε-]) attenuates ROS. This study examined the effects of Myr-PKCε- in kidney I/R. We hypothesized that Myr-PKCε- will exert renal-protective effects by attenuating PKCε translocation in kidney I/R compared to …

Finding The Right Partner: Japan And Netherlands’ Relationship In The Edo Period, Sarah Weeks '24

Senior Scholarly and Creative Symposium

This thesis paper seeks to enhance the understanding of the Japanese-Dutch relationship during the Edo Period. Despite Japan being a closed country during the Tokugawa Shogunate, it uniquely permitted the Netherlands as the only Western country to have access to trade. This decision was challenging to make due to Japan’s previous failed experiences with the West, such as the Portuguese, whose spread of the Christian religion led to major conflict. When Japan expelled out all Western foreigners, they allowed the Dutch to remain. The Netherlands then became the exclusive Western country allowed in Japan for over 200 years. This relationship …

Can Marketing Transcend Entrenched Gender Biases?, Thinh Nguyen

Markets, Globalization & Development Review

Through a feminist lens, Maclaran and Chatzidakis (2022) challenge conventional assumptions about gender, emphasizing its performative nature shaped by social practices rather than inherent traits. This commentary extends analyses of key themes such as gender performativity, the male gaze, and subject-object binaries within marketing. It critiques how marketing strategies reinforce existing power imbalances and systemic biases rooted in historical narratives. The writing also reflects on media interpretations of gender issues through films like 'Turning Red' and 'Barbie'. By contextualizing gendered marketing within broader societal frameworks, this writing contributes to ongoing dialogues in media studies, sociology, and gender studies, highlighting the …

Letras 16

Letras

No abstract provided.