Digital Commons Network^™

Transcription Factor Ap-2 Gamma Affects Porcine Early Embryo Development By Regulating Epigenetic Modification., Daoyu Zhang, Di Wu, Sheng Zhang, Meng Zhang, Yongfeng Zhou, Xinglan An, Qi Li, Ziyi Li

Faculty Research 2024

RESEARCH QUESTION: What is the role and mechanism of action of transcription factor AP-2 gamma (TFAP2C) in porcine early embryo development?

DESIGN: TFAP2C siRNA were injected into porcine oocytes, which subsequently underwent IVF. Different stages of embryos were collected for RNA sequencing, quantitative polymerase chain reaction, immunofluorescence staining to explore the affects in gene expression and epigenetic modification. Porcine fetal fibroblasts were transfected with siRNA, and cells were collected for chromatin immunoprecipitation and dual luciferase reporter assays.

RESULTS: The deficiency of TFAP2C led to disorders in early embryonic development; 1208 genes were downregulated and 792 genes were upregulated in TFAP2C …

Loss Of Sting Impairs Lactogenic Differentiation., Ramiah R Vickers, Garhett L Wyatt, Lilia Sanchez, Jordyn J Vanportfliet, A Phillip West, Weston W Porter

Faculty Research 2024

Heightened energetic and nutrient demand during lactogenic differentiation of the mammary gland elicits upregulation of various stress responses to support cellular homeostasis. Here, we identify the stimulator of interferon genes (STING) as an immune supporter of the functional development of mouse mammary epithelial cells (MECs). An in vitro model of MEC differentiation revealed that STING is activated in a cGAS-independent manner to produce both type I interferons and proinflammatory cytokines in response to the accumulation of mitochondrial reactive oxygen species. Induction of STING activity was found to be dependent on the breast tumor suppressor gene single-minded 2 (SIM2). Using mouse …

Presynaptic Nrxn3 Is Essential For Ribbon-Synapse Maturation In Hair Cells., Alma Jukic, Zhengchang Lei, Elizabeth R Cebul, Katherine Pinter, Yommi Tadesse, Amandine Jarysta, Sandeep David, Natalie Mosqueda, Basile Tarchini, Katie Kindt

Faculty Research 2024

Hair cells of the inner ear and lateral-line system rely on specialized ribbon synapses to transmit sensory information to the central nervous system. The molecules required to assemble these synapses are not fully understood. We show that Nrxn3, a presynaptic adhesion molecule, is crucial for ribbon-synapse maturation in hair cells. In both mouse and zebrafish models, the loss of Nrxn3 results in significantly fewer intact ribbon synapses. We show in zebrafish that, initially, Nrxn3 loss does not alter pre- and postsynapse numbers but, later, synapses fail to pair, leading to postsynapse loss. We also demonstrate that Nrxn3 subtly influences synapse …

Obo Foundry Food Ontology Interconnectivity, Damion Dooley, Liliana Andres-Hernandez, Georgeta Bordea, Leigh Carmody, Duccio Cavalieri, Lauren Chan, Pol Castellano-Escuder, Carl Lachat, Fleur Mougin, Francesco Vitali, Chen Yang, Magalie Weber, Hande Kucuk Mcginty, Matthew Lange

Faculty Research 2024

No abstract provided.

Machine-Guided Design Of Cell-Type-Targeting Cis-Regulatory Elements., Sager J Gosai, Rodrigo Castro, Natalia Fuentes, John C Butts, Kousuke Mouri, Michael Alasoadura, Susan Kales, Thanh Thanh L Nguyen, Ramil R Noche, Arya S Rao, Mary T Joy, Pardis C Sabeti, Steven K Reilly, Ryan Tewhey

Faculty Research 2024

Cis-regulatory elements (CREs) control gene expression, orchestrating tissue identity, developmental timing and stimulus responses, which collectively define the thousands of unique cell types in the body1–3 . While there is great potential for strategically incorporating CREs in therapeutic or biotechnology applications that require tissue specificity, there is no guarantee that an optimal CRE for these intended purposes has arisen naturally. Here we present a platform to engineer and validate synthetic CREs capable of driving gene expression with programmed cell-type specificity. We take advantage of innovations in deep neural network modelling of CRE activity across three cell types, efficient in silico …

Natasha, Pierre, & The Great Comet Of 1812, Otterbein Theatre And Dance Department

2024-2025 Season Productions

No abstract provided.

Neurosteroids For The Treatment Of Major Depressive Disorder (Mdd) And Chronic Pain (Paper), Meghan Haan

Honors Capstone Projects

Neurosteroids are endogenous steroids within the brain that act as modulators in neurotransmission. Among their targets are the gamma-aminobutyric acid (GABA) receptors, which are responsible for inhibitory signaling in the brain. The interaction between neurosteroids and GABA receptors is complex, influencing mood regulation and pain perception. Endogenous neurosteroids have been used in conditions such as postpartum depression (PPD), where fluctuation in neurosteroid levels contribute to the onset of depressive systems. Conversely, exogenous neurosteroids, such as brexanolone and zuranolone, have emerged as therapeutic agents for PPD. These compounds act as positive allosteric modulators (PAMs) on GABA receptors, enhancing inhibitory neurotransmission and …

Stockfeed Labelling In Western Australia, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The Department of Primary Industries and Regional Development (DPIRD) is responsible for regulating the labels of stockfeed supplied in Western Australia (WA) to ensure they provide the required information to people feeding it to their livestock. This factsheet contains information to help stockfeed retailers and manufacturers meet WA’s labelling requirements.

Cultural Perspectives On Psychoactive Substances: A Study Of Psilocybin In Indonesia, Parmis Mazdeh

Indonesia: Arts, Religion, and Social Change

This study examines the various cultural views on psilocybin in Indonesia, emphasizing its role in religious, spiritual, and cultural contexts. It investigates both the historical and present-day use of psychoactive substances, specifically psilocybin, in connecting with the divine, attaining transcendental enlightenment, and shaping identity within specific civilizations. Although psilocybin is illegal in Indonesia, these mushrooms can be found in the Gili Islands, raising questions about how they fit into local spiritual traditions and the legal loopholes that allow their use. Field research in the Gili Islands reveals how religious beliefs and practices influence the cultural acceptance of magic mushrooms, while …

Issue 11: Pulmonary & Critical Care Insider, Bharat Bajantri Md, Sarah Ellsworth-Hoffman Mls, Dustin D. Linn Pharmd, Payal Shukla Md, Ms, Patricia Rich Md, Morgan Crites Np-C

Critical Care & Pulmonary

Pulmonary & Critical Care Insider Issue 11

Compiled by Bharat Bajantri, MD, and librarian Sarah Ellsworth, MLS for the clinicians of the Pulmonary and Critical Care team of Parkview.

The Pulmonary & Critical Care Insider newsletter was created by Dr. Bharat Bajantri, MD and Sarah Ellsworth, MLS in 2023 as a form of current awareness for current practice at our hospital, Parkview Health.

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Topics:

VIEWPOINTS:

Blood Pressure Measurement

Home Noninvasive Ventilation

ORIGINAL STUDY SUMMARY

Acute Pulmonary Embolism

The PEXIVAS trial

Respiratory Failure in hemorrhagic shock

SNAPSHOTS:

RBC Transfusions

New Small cell lung cancer Treatment.

Board Of Pharmacy, Catherine Santos, Marcus Friedman

California Regulatory Law Reporter

No abstract provided.

Delivery Of Tgfβ3 From Magnetically Responsive Coaxial Fibers Reduces Spinal Cord Astrocyte Reactivity In Vitro, Jessica L. Funnell, Jasper Fougere, Diana Zahn, Silvio Dutz, Ryan J. Gilbert

Chemical and Biochemical Engineering Faculty Research & Creative Works

A spinal cord injury (SCI) compresses the spinal cord, killing neurons and glia at the injury site and resulting in prolonged inflammation and scarring that prevents regeneration. Astrocytes, the main glia in the spinal cord, become reactive following SCI and contribute to adverse outcomes. The anti-inflammatory cytokine transforming growth factor beta 3 (TGFβ3) has been shown to mitigate astrocyte reactivity; however, the effects of prolonged TGFβ3 exposure on reactive astrocyte phenotype have not yet been explored. This study investigates whether magnetic core-shell electro spun fibers can be used to alter the release rate of TGFβ3 using externally applied magnetic fields, …

Wind Shear Induced Tornados With Full Thermodynamic Effects, Kakkattukuzhy M. Isaac

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Wind shear-induced Tornado behavior under full thermodynamic formulation using the ideal gas equation is investigated. The computational fluid dynamics model that includes the energy equation is used to obtain solutions of a natural-scale tornado under pressure and temperature boundary conditions that account for their variations vs. altitude in the atmosphere. The present results show significant differences from previous isothermal simulations and those using the Boussinesq model for buoyancy under small temperature differences. Results show that the downdraft observed in tornados originating from supercells, often accompanied by rain and hail can be captured using the present model that simulates the genesis, …

2024-2025 Book-In-Common Teaching Guide, Collin College

Book in Common

EDITOR’S INTRODUCTION

As always, the 2024 - 2025 Teaching Guide exemplifies the Collin College Book-in Common Committee’s commitment to the goal of a year-long, college-wide engagement with an important book and its author. This dystopian novel discusses themes of art, culture, survival, relationships, health, and memory. Highlights of this year’s Guide include:

• New for 2024 – 2025
• Fall 2024 Assessment-aligned activities for COAT Core Objective Personal Responsibility
• Review the interactive teaching strategies and access scaffolding activities
• Responses to the text including explorations of the major themes in Station Eleven, reflection prompts, subject assignments, and connections to art, biology, and …

The Chronicle, Lara Jones, Taysia Bettis, Bella Perez, Joyanna Huang, Kasey Andrews, Emily Riley, Samantha Russell, Corey Suckow, Andy Langager

Campus Chronicle

No abstract provided.

Ua64/2 The Challenge: Magazine For The Center For Gifted Studies (No. 57 Fall 2024), Elizabeth Joyce Editor

Gifted Studies Publications

Magazine created by and about the WKU Center for Gifted Studies.

Torture And Its Sequelae Among Prostituted Women In The United States, Melissa Farley, M. Alexis Kennedy

Criminal Justice Faculty Research

Background: Extreme violence and psychological abuse have been extensively documented and are pervasive in prostitution. Survivors of prostitution report high levels of posttraumatic stress disorder, dissociation, depression, and self-loathing. These are the same sequelae reported by torture survivors.

Objective: Severe forms of violence have been categorized as torture by experts. The authors note that torture is commonly suffered during prostitution and should be appropriately named.

Method: Using standardized measures and including a new measure of torture, we interviewed 45 women in the United States about their torture experiences in prostitution and their symptoms of PTSD, dissociation, childhood trauma, health status …

Boise State Magazine, Anna Webb (Editor)

FOCUS on Boise State

No abstract provided.

Protecting Coastal Communities And Marine Biodiversity Under The Coastal Zone Management Act, Eric V. Hull

South Carolina Law Review

No abstract provided.

2024 October, Morehead State University. Office Of Communications & Marketing.

Morehead State Press Release Archive, 1961 to the Present

Press releases for October of 2024.

Personality (O.C.E.A.N) Driven Data Augmentation For Post-Trial Interviews In A Three-Arm Chronic Back Pain Randomized Clinical Trial, Kamakshi Moparthi

Dartmouth College Master’s Theses

This case study investigates the use of Large Language Models (LLMs) and prompt engineering techniques for data augmentation,in the context of post-trial inter- view data (low-resource natural language data) from a randomized clinical trial on Pain Reprocessing Therapy (PRT) for chronic back pain. The limited sample size from the original study necessitates the generation of synthetic interview samples, needed to draw meaningful inferences from the data and to further extend the study. This study explores the efficacy of various prompting techniques in different approaches such as sentiment inversion and personality-based augmentation. The goal is to elicit desired responses from LLMs …

Pathos, Fall 2024, Portland State University. Student Publications Board

Pathos

Editor: Adriana Stanzione

On The Pathogenesis Of Obesity: Causal Models And Missing Pieces Of The Puzzle, Faidon Magkos, Thorkild I.A. Sørensen, David Raubenheimer, Nikhil V. Dhurandhar, Ruth J.F. Loos, Anja Bosy-Westphal, Christoffer Clemmensen, Mads F. Hjorth, David B. Allison, Gary Taubes, Eric Ravussin, Mark I. Friedman, Kevin D. Hall, David S. Ludwig, John R. Speakman, Arne Astrup

Clinical Research Faculty Publications

Application of the physical laws of energy and mass conservation at the whole-body level is not necessarily informative about causal mechanisms of weight gain and the development of obesity. The energy balance model (EBM) and the carbohydrate–insulin model (CIM) are two plausible theories, among several others, attempting to explain why obesity develops within an overall common physiological framework of regulation of human energy metabolism. These models have been used to explain the pathogenesis of obesity in individuals as well as the dramatic increases in the prevalence of obesity worldwide over the past half century. Here, we summarize outcomes of a …

A Retrospective Study Of Virtual Hospital Experiential Learning Across Clerkship And Pre-Clerkship Doctor Of Osteopathic Medicine Students At A Medical School Based In The United States., Lydia Kersh, Anthony Sciuva, Jennifer H. Shaw

PCOM Scholarly Works

INTRODUCTION: During the COVID-19 pandemic, a medical institution in the United States implemented a virtual clerkship rotation to replicate the skills that students would develop during an in-person clinical site rotation. The primary objective of this study was to identify the efficacy and benefits following the implementation of the Virtual Hospital Experiential Learning (VHEL) model, as demonstrated by increased perceived confidence and skill growth among medical students.

METHODS: Cases were presented to five to six medical students each week. On the Monday of the week, students entered a virtual conference platform for a standardized patient encounter. Staff and faculty randomly …

Implementing Social Emotional Strategies In An Early Childhood Setting, Susan Mcclain

Master's Theses & Capstone Projects

This research paper provides a comprehensive overview of studies focusing in on social-emotional skill development in early childhood and its impact on long-term outcomes in emotional regulation, interpersonal relationships, and problem-solving strategies. Drawing on a comprehensive review of existing literature and empirical studies, the research highlights the critical developmental milestones in social-emotional learning (SEL) during the early years and explore effective strategies for fostering these skills within various contexts, including home, preschool, and other early learning settings. The findings suggest that targeted interventions, such as play-based activities and structured SEL programs, significantly enhance children’s ability to navigate social interactions with …

Assistive Technology In Early Childhood For Language And Communication, Katherine Kemp

Master's Theses & Capstone Projects

Students with special needs across the country and the world continue to struggle to gain functional language and communication skills, and build a vocabulary to use throughout life. Research shows that augmentative and alternative communication or speech-generating devices can be a special, useful, and powerful tool for students' language skills. Based on a literature review of augmentative and alternative communication devices, early childhood, and assistive technology, an in-class study was performed in a Virginia Early Childhood Special Education classroom. The results indicate that augmentative and alternative communication devices and assistive technology have a positive impact on students' language and vocabulary …

Precision Interventions Targeting The Maternal Metabolic Milieu For Healthy Pregnancies In Obesity, Alexandra M. Niclou, Hannah E. Cabre, Emily W. Flanagan, Leanne M. Redman

Clinical Research Faculty Publications

Purpose of Review: Entering pregnancy with obesity increases the risk of adverse health outcomes for parent and child. As such, research interventions are largely focused on limiting excess gestational weight gain during pregnancy, especially in those with obesity. Yet, while many lifestyle interventions are successful in reducing GWG, few affect pregnancy outcomes. Here we review work targeting the metabolic milieu instead of focusing solely on weight. Recent Findings: Work done in non-pregnant populations suggests that specifically targeting glucose, triglyceride, and leptin levels or inflammatory makers improves the metabolic milieu and overall health. We posit that precision interventions that include strategies …

Long-Term Effects Of Climate Change On Juvenile Bull Shark Migratory Patterns, Philip Matich, Jeffrey D. Plumlee, Walter Bubley, Tobey H. Curtis, J. Marcus Drymon, Lindsay L. Mullins, Oliver N. Shipley, Thomas C. Tinhan, Mark R. Fisher

Faculty Publications

Seasonal variability in environmental conditions is a strong determinant of animal migrations, but warming temperatures associated with climate change are anticipated to alter this phenomenon with unknown consequences. We used a 40-year fishery-independent survey to assess how a changing climate has altered the migration timing, duration and first-year survival of juvenile bull sharks (Carcharhinus leucas). From 1982 to 2021, estuaries in the western Gulf of Mexico (Texas) experienced a mean increase of 1.55°C in autumn water temperatures, and delays in autumn cold fronts by ca. 0.5 days per year. Bull shark migrations in more northern estuaries concomitantly changed, with departures …

A Quantitative Adverse Outcome Pathway For Embryonic Activation Of The Aryl Hydrocarbon Receptor Of Fishes By Polycyclic Aromatic Hydrocarbons Leading To Decreased Fecundity At Adulthood, Jon A. Doering, Justin Dubiel, Eric Stock, Cameron H. Collins, Ian Frick, Hunter M. Johnson, Christopher M. Lowrey-Dufour, Justin G.P. Miller, Zhe Xia, Gregg T. Tomy, Steve Wiseman

Faculty Publications

Quantitative adverse outcome pathways (qAOPs) describe the response–response relationships that link the magnitude and/or duration of chemical interaction with a specific molecular target to the probability and/or severity of the resulting apical-level toxicity of regulatory relevance. The present study developed the first qAOP for latent toxicities showing that early life exposure adversely affects health at adulthood. Specifically, a qAOP for embryonic activation of the aryl hydrocarbon receptor 2 (AHR2) of fishes by polycyclic aromatic hydrocarbons (PAHs) leading to decreased fecundity of females at adulthood was developed by building on existing qAOPs for (1) activation of the AHR leading to early …

Comparative Transcriptomic Analyses Reveal Differences In The Responses Of Diploid And Triploid Eastern Oysters To Environmental Stress, Rujuta V Vaidya, Sarah Bodenstein, Dildorakhon Rasulova, Jerome F La Peyre, Morgan W Kelly

Faculty Publications

Triploid oysters are commonly used as the basis for production in the aquaculture of eastern oysters along the USA East and Gulf of Mexico coasts. While they are valued for their rapid growth, incidents of triploid mortality during summer months have been well documented in eastern oysters, especially at low salinity sites. We compared global transcriptomic responses of diploid and triploid oysters bred from the same three maternal source populations at two different hatcheries and outplanted to a high (annual mean salinity = 19.4 ± 6.7) and low (annual mean salinity = 9.3 ± 5.0) salinity site. Oysters were sampled …