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Alternatively Spliced Map4 Isoforms Have Key Roles In Maintaining Microtubule Organization And Skeletal Muscle Function, Lathan Lucas, Larissa Nitschke, Brandon Nguyen, James A Loehr, George G Rodney, Thomas A Cooper

Faculty, Staff and Students Publications

Skeletal muscle cells (myofibers) are elongated non-mitotic, multinucleated syncytia that have adapted a microtubule lattice. Microtubule-associated proteins (MAPs) play roles in regulating microtubule architecture. The most abundant MAP in skeletal muscle is MAP4. MAP4 consists of a ubiquitous MAP4 isoform (uMAP4), expressed in most tissues, and a striated-muscle-specific alternatively spliced isoform (mMAP4) that includes a 3,180-nucleotide exon (exon 8). To determine the role of mMAP4 in skeletal muscle, we generated mice that lack mMAP4 and express only uMAP4 due to genomic deletion of exon 8. We demonstrate that loss of mMAP4 leads to disorganized microtubule architecture and intrinsic loss of …

Curcumin Reduces Malondialdehyde And Improves Antioxidants In Humans With Diseased Conditions: A Comprehensive Meta-Analysis Of Randomized Controlled Trials, Mohammad Alizadeh, Sorayya Kheirouri

BioMedicine

Objective: This systematic review and meta-analysis was conducted to collate the effects of curcumin on MDA and antioxidant markers in individuals with diseased conditions. In this study the research question was “does curcumin supplementation improves oxidative stress and antioxidant defense enzymes in human subjects compared to a group without curcumin supplementation?

Methods: This research included randomized controlled trials published in English in any year, in which intervention with curcumin was compared to either placebo, or standard of care or no intervention. Pubmed, Embase, Cochrane Central, Scopus and Google Scholar were searched. Meta-analysis was performed using RevMan (version 5.3), with standardized …

Epigenetic Profiling Of Mutyh, Klf6, Wnt1 And Klf4 Genes In Carcinogenesis And Tumorigenesis Of Colorectal Cancer, Kosar Babaei, Roya Khaksar, Tahereh Zeinali, Hossein Hemmati, Ahmadreza Bandegi, Pirouz Samidoust, Mohammad Taghi Ashoobi, Hooman Hashemian, Kourosh Delpasand, Fereshteh Talebinasab, Hoora Naebi, Seyed Hossein Mirpour, Arman Keymoradzadeh, Seyedeh Elham Norollahi

BioMedicine

Colorectal cancer (CRC) is distinguished by epigenetic elements like DNA methylation, histone modification, histone acetylation and RNA remodeling which is related with genomic instability and tumor initiation. Correspondingly, as a main epigenetic regulation, DNA methylation has an impressive ability in order to be used in CRC targeted therapy. Meaningly, DNA methylation is identified as one of most important epigenetic regulators in gene expression and is considered as a notable potential driver in tumorigenesis and carcinogenesis through gene-silencing of tumor suppressors genes. Abnormal methylation situation, even in the level of promoter regions, does not essentially change the gene expression levels, particularly …

The Rna Degradation Pathway Is Involved In Pparα-Modulated Anti-Oral Tumorigenesis, Nai-Wen Chang, Yi-Ping Huang

BioMedicine

Background: The activation of peroxisome proliferator-activated receptor alpha (PPARα) has been shown to reprogram tumor metabolism and exhibits great potential for treating anti-oral tumorigenesis.

Methods: In this study, we used a pathway-based strategy to explore possible functional pathways involved in the anticancer activity of PPARα in oral cancer cells through next-generation sequencing (NGS) and bioinformatic approaches.

Results: We found that 3919 genes were upregulated and 1060 genes were downregulated through PPARα activation. These genes were mainly involved in the proteasomal, mRNA surveillance, spliceosomal, RNA transport, and RNA degradation pathways, as indicated by GO and KEGG enrichment analysis. Importantly, a total …

Oral Acute And Sub-Acute Toxic Effects Of HydroalcoholicTerminalia ChebulaRetz AndAchillea WilhelmsiiExtracts In Balb/C Mice, Mahnaz Jafari, Kourosh Manochehri Naeini, Zahra Lorigooini, Rasool Namjoo

BioMedicine

Background: This study examined the acute and sub-acute toxic effects ofTerminalia chebulaandAchillea wilhelmsiiextracts on the murine model.

Methods: In both phases, mice were assigned to intervention and control groups. At the end of study, the liver, kidney, and heart tissues were collected for histopathological studies.

Results: In the acute phase of the study, the safe dose was ≤5000 mg/kg for both extracts. In sub-acute phase, LD50 (95% CI) ofAchillea wilhelmsiiextract was determined ≥5000 mg/kg and that ofTerminalia chebulaextract 2754.436 (2438-3114) mg/kg. The highest dose ofT. chebulaextract induced few histopathological changes.

Conclusion: It will be useful to gain information on the …

The Potential Therapeutic Effect For Melatonin And Mesenchymal Stem Cells On Hepatocellular Carcinoma, Yasser Mohamed, Mohamed A. Basyony, Nabila I. El-Desouki, Walied S. Abdo, Mohammed A. El-Magd

BioMedicine

Background/aim: Herein, we investigated the potential therapeutic effect of Melatonin (Mel) and/or mesenchymal stem cells (MSCs) on rat model of HCC.

Materials and Methods: Female mature rats were divided into 5 groups (n= 10/group): normal (Nor), HCC group intraperitoneally injected with 200 mg/kg DEN, and 3 treated groups; HCC + Mel (Mel) group given Mel intraperitoneally 20 mg/kg, twice a week, HCC + MSCs (MSCs) group intravenously injected by 1 × 10⁶ cells, and HCC + MSCs (Mel +MSCs) group.

Results: Rats in HCC group showed most deteriorated effect in form of increased mortality and relative liver weight, elevated …

Development Of Broad Spectrum Mycosporine Loaded Sunscreen Formulation From Ulva Fasciata Delile, Saurabh Bhatia, Satish Sardana, Ajay Sharma, Celia Bertha Vargas De La Cruz, Bhupal Chaugule, Laleh Khodaie

BioMedicine

Ba.ckground: Sunscreen formulations primarily offer protection against UV induced damages however nowadays it also maintains skin natural physiological conditions. Current global market is flooded with numerous sunscreen products which offer protection to skin against several UV induced damages. However most of these sunscreen formulations offers narrow spectrum protection against UV and also suffer from stability as well as toxicity related issues.

Methods: Present work aims to isolate mycosporine amino acid (Mgy) from green alga namelyUlva fasciata (U. fasciata)and study its sunscreen potential against widely used domestic marketed formulation. Stability evaluations were also performed for almost 90 days.

Results: Results demonstrated …

Protective Effect OfGlycyrrhiza GlabraRoots Extract On Bone Mineral Density Of Ovariectomized Rats, Dimitrios Galanis, Konstantinos Soultanis, Pavlos Lelovas, Alexandros Zervas, Panagiotis Papadopoulos, Antonis Galanos, Katerina Argyropoulou, Maria Makropoulou, Anastasia Patsaki, Christina Passali, Anastasia Tsingotjidou, Stavros Kourkoulis, Sofia Mitakou, Ismene Dontas

BioMedicine

Objective: The aim of this study was to evaluate the potential effect of the methanolic extract of plantGlycyrrhiza glabraroots on bone mineral density and femoral bone strength of ovariectomized rats.

Methods: Thirty 10-month-old Wistar rats were randomly separated into three groups of ten, Control, Ovariectomy and Ovariectomy-plus-Glycyrrhiza in their drinking water. Total and proximal tibial bone mineral density was measured in all groups before ovariectomy (baseline) and after 3 and 6 months post ovariectomy. Three-point-bending of the femurs and uterine weight and histology were examined at the end of the study.

Results: No significant difference was noted in bone density …

In Vivo Microdialysis And In Vitro Hplc Analysis Of The Impact Of Paeoniflorin On The Monoamine Levels And Their Metabolites In The Rodent Brain, Yuh-Tzy Lin, Wei-Shih Huang, Huei-Yann Tsai, Min-Min Lee, Yuh-Fung Chen

BioMedicine

Background: Paeoniflorin (PF) possesses several effects such as analgesic, the anti-spasmodic effect on smooth muscle. It protects the cardiovascular system and reveals the neuroprotective effect on cerebral ischemia. Monoamine system has been identified to have complex regulatory effects in pain signaling. There are no reports regarding the impact of PF on monoamine levels in the rodent brain by microdialysis. In this study, the effects of PF on monoamines and their metabolites in the rodent brain usingin vivomicrodialysis andin vitrohigh performance liquid chromatography (HPLC) analysis.

Methods: Male S.D. rats were anesthetized, fixed onto the stereotaxic instrument to identify the positions of …

Gynura BicolorAqueous Extract Attenuated H2O2Induced Injury In Pc12 Cells, Ya-Chen Yang, Wen-Tzu Wu, Mei-Chin Mong, Zhi-Hong Wang

BioMedicine

Background: Protective effects ofGynura bicoloraqueous extract (GAE) at three concentrations upon nerve growth factor (NGF) differentiated-PC12 cells against H₂O₂induced injury were examined.

Methods: NGF differentiated-PC12 cells were treated with GAE at 0.25%, 0.5% or 1%. 100 μM H₂O₂was used to treat cells with GAE pre-treatments. After incubating at 37 °C for 12 hr, experimental analyses were processed.

Results: H₂O₂exposure decreased cell viability, increased plasma membrane damage, suppressed Bcl-2 mRNA expression and enhanced Bax mRNA expression. GAE pre-treatments reversed these changes. H₂O₂exposure reduced mitochondrial membrane …

Association Between Colorectal Cancer And Thiazolidinediones Administration In A Case-Control Study, Kuan-Fu Liao, Cheng-Li Lin, Shih-Wei Lai

BioMedicine

No abstract provided.

In VitroAntioxidant And Antinociceptive Properties OfPorphyra Vietnamensis, Saurabh Bhatia, Satish Sardana, Kishan Ram Senwar, Anjali Dhillon, Ajay Sharma, Tanveer Naved

BioMedicine

No abstract provided.

Materials And Structures Used In Meniscus Repair And Regeneration: A Review, Ketankumar Vadodaria, Abhilash Kulkarni, E Santhini, Prakash Vasudevan

BioMedicine

No abstract provided.

A Brief Literature Review And Own Clinical Experience In Prophylaxis Of Oral Mucositis In Children Using Low Level Laser Therapy, Sergey Moskvin, Denis Pritiko, Elena Sergeenko, Elena Lukash, Leonid Gusev

BioMedicine

No abstract provided.

The Effect Of Selenium Supplementation On Cystine Crystal Volume In Patients With Cystinuria, Mehrdad Mohammadi, Amin Shohani, Hatef Khorami, Kia Nouri Mahdavi, Mohammad Hossein Izadpanahi, Farshid Alizadeh, Mohammad Azizi

BioMedicine

No abstract provided.

Mechanical Stretch And Chronotherapeutic Techniques For Progenitor Cell Transplantation And Biomaterials, Eve Helena Rogers, Vanja Pekovic-Vaughan, John Alan Hunt

BioMedicine

No abstract provided.

A Screening Of Growth Inhibitory Activity Of Iranian Medicinal Plants On Prostate Cancer Cell Lines, Majid Asadi-Samani, Mahmoud Rafieian-Kopaei, Zahra Lorigooini, Hedayatollah Shirzad

BioMedicine

No abstract provided.

Effectiveness Of Low Level Laser Therapy For Treating Male Infertility, Sergey Vladimirovich Moskvin, Oleg Ivanovich Apolikhin

BioMedicine

No abstract provided.

Content Of Nitric Oxide And Glycative Compounds In Cured Meat Products-Negative Impact Upon Health, Ya-Chen Yang, Zhi-Hong Wang, Mei-Chin Yin

BioMedicine

No abstract provided.

The Molecular Mechanism Of Contrast-Induced Nephropathy (Cin) And Its Link To In Vitro Studies On Iodinated Contrast Media (Cm), Jai-Sing Yang, Yan-Ru Peng, Shih-Chang Tsai, Yeu-Sheng Tyan, Chi-Cheng Lu, Hong-Yi Chiu, Yu-Jen Chiu, Sheng-Chu Kuo, Yuh-Feng Tsai, Ping-Chin Lin, Fuu-Jen Tsai

BioMedicine

No abstract provided.

Only Lasers Can Be Used For Low Level Laser Therapy, Sergey Vladimirovich Moskvin

BioMedicine

No abstract provided.

The Anti-Inflammatory And Anti-Glycative Effects Of Rosmarinic Acid In The Livers Of Type 1 Diabetic Mice, Yu-Ju Wen, Mei-Chin Yin

BioMedicine

No abstract provided.

Mesh Materials And Hernia Repair, Santhini Elango, Sakthivel Perumalsamy, Krishnakumar Ramachandran, Ketankumar Vadodaria

BioMedicine

No abstract provided.

Transglutaminase 2 In Human Diseases, Zsuzsa Szondy, Ilma Korponay-Szabó, Robert Király, Zsolt Sarang, Gregory J. Tsay

BioMedicine

No abstract provided.

Torenia ConcolorLindley Var.FormosanaYamazaki Extracts Improve Inflammatory Response And Lipid AccumulationViaPpars Activation, Yu-Chia Liang, Jun-Cheng Hu, Pei-Ying Li, Guan-Jhong Huang, Yueh-Hsiung Kuo, Che-Yi Chao

BioMedicine

No abstract provided.

Genetic Characteristic Of Class 1 Integrons In Proteus Mirabilis Isolates From Urine Samples, Chih-Ming Chen, Chih-Ho Lai, Hwa-Jene Wu, Lii-Tzu Wu

BioMedicine

No abstract provided.

Potential Phytoestrogen Alternatives Exert Cardio-Protective MechanismsViaEstrogen Receptors, Marthandam Asokan Shibu, Wei-Wen Kuo, Chia-Hua Kuo, Cecilia-Hsuan Day, Chia-Yao Shen, Li-Chin Chung, Chao-Hung Lai, Lung-Fa Pan, V. Vijaya Padma, Chih-Yang Huang

BioMedicine

No abstract provided.

Alleviative Effects From Boswellic Acid On Acetaminophen-Induced Hepatic Injury, Lung-Che Chen, Li-Hong Hu, Mei-Chin Yin

BioMedicine

No abstract provided.

Effects Of Swimming Exercise On Nerve Regeneration In A Rat Sciatic Nerve Transection Model, Chien-Fu Liao, Tse-Yen Yang, Yung-Hsiang Chen, Chun-Hsu Yao, Tzong-Der Way, Yueh-Sheng Chen

BioMedicine

No abstract provided.

Kuei-Lu-Er-Xian-Jiao Extract Enhances Bmp-2 Production In Osteoblasts, Min-Huan Wu, Ting-Hsuan Lee, Hsiang-Ping Lee, Te-Mao Li, I-Tee Lee, Po-Chuen Shieh, Chih-Hsin Tang

BioMedicine

No abstract provided.