Digital Commons Network^™

Infant-Derived Human Nasal Organoids Exhibit Relatively Increased Susceptibility, Epithelial Responses, And Cytotoxicity During Rsv Infection, Gina M Aloisio, Divya Nagaraj, Ashley M Murray, Emily M Schultz, Trevor Mcbride, Letisha Aideyan, Erin G Nicholson, David Henke, Laura Ferlic-Stark, Anubama Rajan, Amal Kambal, Hannah L Johnson, Elina Mosa, Fabio Stossi, Sarah E Blutt, Pedro A Piedra, Vasanthi Avadhanula

Faculty, Staff and Students Publications

Background: Respiratory syncytial virus (RSV) causes significant morbidity and mortality, especially in young children. Why RSV infection in children is more severe compared to healthy adults is not fully understood.

Methods: We used ex-vivo human nasal organoid platforms from infants and adults to investigate the underlying mechanism of this disease disparity at the initial site of RSV replication, the nasal epithelium.

Results: Infant-derived human nasal organoid-air liquid interface (HNO-ALIs) lines were more susceptible to early RSV replication. Moreover, infant-derived HNO-ALIs elicited a statistically significant greater overall cytokine response, enhanced mucous production, and greater cellular damage compared to their adult counterparts. …

A Transgenic Mouse Model Of Down Syndrome Acute Lymphoblastic Leukemia Identifies Targetable Vulnerabilities, Jacob J Junco, Max Rochette, Michelle Alozie, Raushan Rashid, Maci Terrell, Barry Zorman, Pavel Sumazin, Lauren Rowland, Gino Dettorre, Reid T Powell, Clifford C Stephan, Peter J Davies, Margarita Martinez-Moczygemba, Jun J Yang, Karen R Rabin

Faculty, Staff and Students Publications

No abstract provided.

Testosterone Acts Through The Membrane Protein Gprc6a To Cause Cardiac Edema In Zebrafish Embryos, Vahid Zadmajid, Shayan Shahriar, Daniel A Gorelick

Faculty, Staff and Students Publications

Androgens are classically thought to act through intracellular androgen receptors (AR/NR3C4), but they can also trigger non-genomic effects via membrane proteins. Although several membrane androgen receptors have been characterized in vitro, their functions in vivo remain unclear. Using a chemical-genetic screen in zebrafish, we found that GPRC6A, a G-protein-coupled receptor, mediates non-genomic androgen actions during embryonic development. Exposure to androgens (androstanedione, DHT and testosterone) caused cardiac edema or tail curvature in wild-type embryos, as well as in ar mutants, suggesting AR-independent pathways. We then mutated putative membrane androgen receptors [gprc6a, hcar1-4 and zip9 (slc39a9)] and found that only gprc6a mutants …

The Bacterial Cell Wall Components Lipopolysaccharide And Peptidoglycan Initiate Divergent Local Tissue And Systemic Inflammatory Response Profiles In The Chicken Model, Kristen A. Byrne, Gisela F. Erf

Poultry Science Faculty Publications and Presentations

The innate immune system plays an important role in the early defense against pathogens. To better understand the chicken’s innate inflammatory responses, we concurrently examined the cellular responses at the injection site and in the blood following intradermal (i.d.) injections of lipopolysaccharide (LPS) or peptidoglycan (PGN) into the pulp of growing feathers (GFs). Both LPS from Gram-negative bacteria and PGN from Gram-positive bacteria are potent stimulators of innate inflammatory responses. The time-course study conducted revealed similarly elevated levels of heterophils and macrophages in injected GF-pulps during the first 24 h for both LPS and PGN. However, PGN also stimulated rapid …

2022 Carroll County Advisory Council Minutes And Agendas, Mikayla Cerney

UNH Cooperative Extension

No abstract provided.

2024 Carroll County Advisory Council Minutes And Agendas, Mikayla Cerney

UNH Cooperative Extension

No abstract provided.

Pathogenicity And Virulence Of Human Respiratory Syncytial Virus: Multifunctional Nonstructural Proteins Ns1 And Ns2, Trudy N Merritt, Jingjing Pei, Daisy W Leung

2020-Current year OA Pubs

Human respiratory syncytial virus (hRSV) is a major cause of lower respiratory tract infections in young children, the elderly, and immunocompromised. Despite its discovery over 60 years ago and the global impact on human health, limited effective prophylactic or therapeutic options have been available for hRSV infections. This has largely been attributed to the legacy of vaccine failure in the 1960s using a formalin-inactivated RSV , which led to enhancement of disease post exposure to hRSV infection and hampered subsequent development of vaccine candidates. Recent FDA approval of vaccines for older adults and pregnant individuals are major advancements but leaves …

The Sodium-Proton Exchangers Snhe And Nhe1 Control Plasma Membrane Hyperpolarization In Mouse Sperm, Analia G Novero, Celia M Santi, Et Al.

2020-Current year OA Pubs

Sperm capacitation is a complex process that takes place in the female reproductive tract and empowers mammalian sperm with the competence to fertilize an egg. It consists of an intricate cascade of events that can be mimicked in vitro through incubation in a medium containing essential components, such as bicarbonate, albumin, Ca

Development And Validation Of A Prechiasmatic Mouse Model Of Subarachnoid Hemorrhage To Measure Long-Term Cognitive Deficits, Deepti Diwan, Jogender Mehla, James W Nelson, James D Quirk, Sheng-Kwei Song, Sarah Cao, Benjamin Meron, Aminah Mostofa, Gregory J Zipfel

2020-Current year OA Pubs

Controllable and reproducible animal models of aneurysmal subarachnoid hemorrhage (SAH) are crucial for the systematic study of the pathophysiology and treatment of this debilitating condition. However, current animal models have not been successful in replicating the pathology and disabilities seen in SAH patients, especially the long-term neurocognitive deficits that affect the survivor's quality of life. Therefore, there is an unmet need to develop experimental models that reliably replicate the long-term clinical ramifications of SAH - especially in mice where genetic manipulations are straightforward and readily available. To address this need, a standardized mouse SAH model is developed that reproducibly produced …

2024 European Heart Rhythm Association/Heart Rhythm Society/Asia Pacific Heart Rhythm Society/Latin American Heart Rhythm Society Expert Consensus Statement On Catheter And Surgical Ablation Of Atrial Fibrillation, Stylianos Tzeis, Ralph J Damiano, Et Al.

2020-Current year OA Pubs

In the last three decades, ablation of atrial fibrillation (AF) has become an evidence-based safe and efficacious treatment for managing the most common cardiac arrhythmia. In 2007, the first joint expert consensus document was issued, guiding healthcare professionals involved in catheter or surgical AF ablation. Mounting research evidence and technological advances have resulted in a rapidly changing landscape in the field of catheter and surgical AF ablation, thus stressing the need for regularly updated versions of this partnership which were issued in 2012 and 2017. Seven years after the last consensus, an updated document was considered necessary to define a …

Tyk2 Regulates Tau Levels, Phosphorylation And Aggregation In A Tauopathy Mouse Model, Jiyoen Kim, Bakhos Tadros, Yan Hong Liang, Youngdoo Kim, Cristian Lasagna-Reeves, Jun Young Sonn, Dah-Eun Chloe Chung, Bradley Hyman, David M Holtzman, Huda Yahya Zoghbi

2020-Current year OA Pubs

Alzheimer's disease is one of at least 26 diseases characterized by tau-positive accumulation in neurons, glia or both. However, it is still unclear what modifications cause soluble tau to transform into insoluble aggregates. We previously performed genetic screens that identified tyrosine kinase 2 (TYK2) as a candidate regulator of tau levels. Here we verified this finding and found that TYK2 phosphorylates tau at tyrosine 29 (Tyr29) leading to its stabilization and promoting its aggregation in human cells. We discovered that TYK2-mediated Tyr29 phosphorylation interferes with autophagic clearance of tau. We also show that TYK2-mediated phosphorylation of Tyr29 facilitates pathological tau …

Disputing Personal Data In The Eu-China Comprehensive Agreement On Investment, Mark Mclaughlin

Research Collection Yong Pung How School Of Law

This chapter analyses the emerging data realms in China and the European Union against the backdrop of the Comprehensive Agreement on Investment (CAI). It examines the link between data protection and investment flows and undertakes a comparative analysis of the PIPL and GDPR. Moreover, this chapter will also analyse the Digital Silk Road in promoting China’s model of data sovereignty. While early trade and investment agreements did not consider data relevant for the regulation of cross-border commerce, modern treaties are increasingly cognisant of rules for data protection. As such, the data-related provisions of the CAI will be placed in the …

A Proxy For Piety: A Closer Look At Religious Cost In The Substantial Burden Inquiry, Philip Andrew B. Wines

Fordham Law Review Online

This Comment discusses whether the Establishment Clause and “religious question” doctrine prohibit courts from considering the subjective religious harm suffered by free exercise claimants when determining if laws impose a “substantial burden” on the claimant, as defined by the Religious Freedom Restoration Act (RFRA) and the Religious Land Use and Institutionalized Persons Act (RLUIPA). It explores a dilemma that courts are presently facing. They must choose either to wade into constitutionally perilous theological debates to decide cases on their merits, or to defer to free exercise claimants on their own assertions of substantial burden and risk swallowing up the law …

Canola In Prairie Canada: Its Development As A Regional Crop And Its Challenge To Wheat, Thomas A. Rumney

The Geographical Bulletin

Canola (Brassica napus), a variety of rapeseed used for edible vegetable oils, animal feed, and industrial oil products, is a relatively new addition to the repertoire of crops grown by farmers in the Canadian Prairies. Becoming common only after approximately 1960. Canadian canola production has been concentrated in Manitoba, Saskatchewan, and Alberta, and has become a serious rival to wheat in terms of acreage and production levels. This paper examines the growth and development of this regional agricultural phenomenon from the poi nt of view of its location and distributional characteristics.

Undermining Our Best Nature: A Defense Of Dysart Woods, Scott Ruescher

Appalachia

No abstract provided.

At Hikers Believe In Magic, Roger Sheffer

Appalachia

No abstract provided.

The Real Cold Mountain In Pisgah National Forest, Danny Bernstein

Appalachia

No abstract provided.

Accidents

Appalachia

No abstract provided.

Tundra Frogs: Previously Unrecorded Amphibian Life In The Northeast's Alpine Zone, Michael Jones

Appalachia

No abstract provided.

Black Bears: Beyond The Myth, Sandy Stott

Appalachia

No abstract provided.

"The Best Job In State Government": Buzz Caverly Reflects On His Career As Director Of Maine's Baxter State Park, Christine Woodside

Appalachia

No abstract provided.

Little Lyford Pond Camps: From Frontier Logging To Backcountry Bliss At A Traditional Maine Sporting Camp, Sarah Jane Shangraw

Appalachia

No abstract provided.

Appalachia Summer/Fall 2005: Complete Issue

Appalachia

No abstract provided.

Liberty To The Captive: The Two Definitions Of Equality In The Abolitionist Movement, Justin Searls

Bound Away: The Liberty Journal of History

The matter of the debate on equality’s definition between black Christian ministers and white Christian ministers is an understudied topic of discourse that merits attention in the historical profession. In recent years, there has been discussion surrounding the general racial divide within abolitionism. However, the religious perspective on this matter has received little to no attention. How did black ministers and white ministers address the question of emancipation and enfranchisement for African Americans in the abolitionist movement from the 1830s through the Civil War and beyond? There is a significant disparity in the discussion of equal rights for African Americans …

Books Of Note

Appalachia

No abstract provided.

Laying Waste To The Land To Produce Electricity: Rupert River Projec Could Be An Important Tipping Point, Paul Rudershausen

Appalachia

No abstract provided.

A Light In Winter Woods, Douglas Haynes

Appalachia

No abstract provided.

Avalanche Training On Alaska's Slopes: Hard Lessons From Arctic Snow, Alys Culhane

Appalachia

No abstract provided.

Mushing In New England: From Commerce To Competition To Cooperation, Sarah Jane Shangraw

Appalachia

No abstract provided.

In Grizzly Country: The Wonders And Hazards Of Hiking Alaska's Brooks Range, Mark Goodreau

Appalachia

No abstract provided.