Digital Commons Network^™

Gonadal Absence Of Pig-1: Exploration Of Pig-1 In The Gonad Of Caenorhabditis Elegans, Winston S. Bennah

Undergraduate Theses

Caenorhabditis elegans are model organisms frequently studied to better understand biological processes. They have short life spans and produce a large number of progeny. Additionally, many of their molecular development processes can be found in human, providing insight into cell death and cell fate. In this project, our research aims to create a triple mutant strain to test the importance of the pig-1 gene in the gonad of C. elegans. This strain carries the following alleles: ckb-3::degron; him-8; gfp::pig-1. The pig-1 gene has been previously studied in neuroblast cells in C. elegans and is required for …

Culturally Responsive Social-Emotional Training And Supplemental Resources For Early Childhood Teachers, Danielle Johnson

Masters Projects

Black students are overrepresented in discipline and behavior referrals in the early childhood setting. Given the increasing need for young black children to learn social emotional skills, how can teachers effectively support them with inadequate curriculum? There is significant research that shows the possible reasons why there is an over representation of black students being labeled as a behavior problem in early childhood educational settings. These reasons can create a negative cycle for black students causing much harm to their education and their views of school. Teachers can attempt to resolve this issue by implementing a culturally responsive Social-Emotional Learning …

Recovery Of Black Carbon Concentrations In Burned Forests In Wyoming, Usa, Monica Vanesa Zapata Villegas

Dissertations and Theses

Burned forests that remain standing following fire release light-absorbing particles (LAPs), including black carbon (BC), into winter snowpacks, reducing snow surface albedo and accelerating snowmelt timing for decades postfire. Although the role of LAPs in seasonal snow has been extensively studied, the spatiotemporal variability of LAPs' contributions to snowmelt relative to time-since-fire and burn severity remains unknown. This study aimed to quantify the spatiotemporal variability of forest fire effects on LAPs in snow and associated snow albedo in the Triple Divide region of western Wyoming, headwaters of the Colorado, Columbia, and Missouri rivers. Using geochemical analysis and radiative transfer modeling, …

Professional Practices(Art 399), Ciro Astarita

Professional Practices (ART 399)

The blending of cultures and the fusion of art mediums are both integral components of contemporary art culture. High art, low art, sculpture, fashion, graphic design, animation, traditional drawing and digital illustration are all coming together in today’s world in interesting and unique ways. This parallels my life growing up as an Italian American, one side of my family being from Italy and the other half from America. Growing up, I was inspired by my love of comics and cartoons as well as my frequent trips to Italy and visiting my nonno and nonna. Italy is considered ‘old’ and America …

A Potent Pan-Sarbecovirus Neutralizing Antibody Resilient To Epitope Diversification, Laura E Rosen, Zhuoming Liu, Sean P J Whelan, Et Al.

2020-Current year OA Pubs

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evolution has resulted in viral escape from clinically authorized monoclonal antibodies (mAbs), creating a need for mAbs that are resilient to epitope diversification. Broadly neutralizing coronavirus mAbs that are sufficiently potent for clinical development and retain activity despite viral evolution remain elusive. We identified a human mAb, designated VIR-7229, which targets the viral receptor-binding motif (RBM) with unprecedented cross-reactivity to all sarbecovirus clades, including non-ACE2-utilizing bat sarbecoviruses, while potently neutralizing SARS-CoV-2 variants since 2019, including the recent EG.5, BA.2.86, and JN.1. VIR-7229 tolerates extraordinary epitope variability, partly attributed to its high binding affinity, …

Automate To Create: Simplifying Workflows In Small Film Productions, Adam Bonny

Undergraduate Honors Theses

This thesis aims to provide guidance for film students and small in- dependent productions on streamlining their film productions, with a particular focus on simplifying the postproduction workflow. While there’s a significant written component to this thesis, it remains classified as a ‘creative project’ and thus may not look like traditional scholarly research of media arts in some as- pects, mainly by focusing on the technical considerations of filmmaking rather than critical study and media theory. It is split into two parts. First, it will detail the research and the decision-making behind the creation of a Notion template aimed at …

The Cowl - V. 88 N. 10 - December 12, 2024

The Cowl

The Cowl - student newspaper of Providence College. Vol. 88 No. 10 - December 12, 2024. 20 pages.

The 2024 Nobel Prize In Physiology Or Medicine: Microrna Takes Center Stage, George A. Calin, Florent Hubé, Michael R. Ladomery, Nicholas Delihas, Manuela Ferracin, Laura Poliseno, Luca Agnelli, Suresh K. Alahari, Ai-Ming Yu, Xiao-Bo Zhong

School of Medicine Faculty Publications

The Non-coding Journal Editorial Board Members would like to congratulate Victor Ambros and Gary Ruvkun, who were jointly awarded the 2024 Nobel Prize in Physiology or Medicine for their groundbreaking discovery of microRNAs and the role of microRNAs in post-transcriptional gene regulation, uncovering a previously unknown layer of gene control in eukaryotes [...].

Predictive Modeling And Sustainability Assessment Of Hydrothermal Liquefaction Of Seaweeds: A Techno-Economic And Life Cycle Analysis Approach, Micheal O. Asama

Mechanical Engineering Theses

This study presents comprehensive predictive models and an economic and environmental assessment of HTL for converting macroalgae into biofuels and valuable co-products. Predictive models were developed based on experimental kinetic data to simulate batch HTL of brown seaweed, capturing the yields of biocrude, gas, biochar, and water-soluble compounds as functions of key process variables, including temperature, time, pressure, and water-to-biomass ratio. Analysis of Variance (ANOVA) confirmed that temperature and residence time significantly affect biocrude yield, with an optimal yield of 23% achieved at 283°C, 200 bar, 54 minutes, and a 10:1 water-to-biomass ratio. The model’s predictive accuracy was validated with …

Safety, Immunogenicity, And Efficacy Of A Recombinant Vesicular Stomatitis Virus Vectored Vaccine Against Severe Fever With Thrombocytopenia Syndrome Virus And Heartland Bandavirus, Philip Hicks, Tomaz B. Manzoni, Jonna B. Westover, Raegan J. Petch, Brianne Roper, Brian B. Gowen, Paul Bates

Animal, Dairy, and Veterinary Science Faculty Publications

Background: Severe fever with thrombocytopenia syndrome virus (STSV) is a recently emerged tickborne virus in east Asia with over 18,000 confirmed cases. With a high case fatality ratio, SFTSV has been designated a high priority pathogen by the WHO and the NIAID. Despite this, there are currently no approved therapies or vaccines to treat or prevent SFTS. Vesicular stomatitis virus (VSV) represents an FDA-approved vaccine platform that has been considered for numerous viruses due to its low sero-prevalence in humans, ease in genetic manipulation, and promiscuity in incorporating foreign glycoproteins into its virions. Methods: In this study, we developed a …

Systemic Antibiotic Selection In Skin And Soft Tissue Infections, Shafira Anindya Purnawan, Windy Keumala Budianti, Sandra Widaty

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Antibiotics are used in daily practice. To properly select antibiotics, it is important to pay attention to the definitive diagnosis, class of antibiotic, dose, interval, route of administration, and exact duration of use.

Discussion: The World Health Organization (WHO) categorizes antibiotics into three groups: Access, Watch, and Reserve—known as the AWaRe classification—to help guide appropriate usage and combat antibiotic resistance. The Gyssens flowchart serves as a valuable tool for clinicians to qualitatively evaluate antibiotic utilization. In skin and soft tissue infections (SSTIs), treatment strategies are tailored according to the characteristics of the infection, including purulence, necrosis, and overall severity. …

Kenyon Collegian - December 12, 2024

The Kenyon Collegian

No abstract provided.

Review – Insect Farming For Food And Feed In The Global South: Focus On Black Soldier Fly Production, K. B. Barragán-Fonseca, D. Gómez, C. H. Lalander, D. Dzepe, S. Y. Chia

All Peer-Reviewed Publications

Clear differences exist between the Global South and the Global North with respect to economic development. The majority of small and medium-sized insect production farms are located in Africa, Asia, and Latin America, which face challenges to food production and organic waste management in general. These regions have a long history of insect use, as well as environmental advantages, making production of insects in general - and the black soldier fly in particular - a promising option for sustainable food production and organic waste management. This study aimed to identify the current state of black soldier fly (Hermetia illucens L.) …

A Survey Of Several Python Libraries Foe Computational Geometry, Meghana Kolluru

Masters Projects

This project delves into computational geometry, a crucial area of computer science, by

implementing algorithms to solve geometric problems using Python libraries like Shapely, SciPy,

and Triangle. It begins with foundational tasks such as creating geometric objects (points, lines,

polygons) and performing operations like calculating distances, intersections, and unions. These

basics lay the groundwork for tackling more advanced applications.

Key implementations include the Convex Hull, which computes the smallest convex

polygon enclosing a set of points, aiding in applications like collision detection and GIS. The

project also explores Voronoi Diagrams, which partition a plane into regions based on proximity,

and …

The 2024 Nobel Prize In Physiology Or Medicine: Microrna Takes Center Stage, George A Calin, Florent Hubé, Michael R Ladomery, Nicholas Delihas, Manuela Ferracin, Laura Poliseno, Luca Agnelli, Suresh K Alahari, Ai-Ming Yu, Xiao-Bo Zhong

Faculty, Staff and Student Publications

The Non-coding Journal Editorial Board Members would like to congratulate Victor Ambros and Gary Ruvkun, who were jointly awarded the 2024 Nobel Prize in Physiology or Medicine for their groundbreaking discovery of microRNAs and the role of microRNAs in post-transcriptional gene regulation, uncovering a previously unknown layer of gene control in eukaryotes [...].

Emerging Aquatic Plant Threats To Oregon Waterways, Jacob Rose, Gabriel Campbell

Center for Lakes and Reservoirs Publications and Presentations

This brief presentation provides an overview some of two invasive aquatic plants that pose a threat to the Columbia River, where it borders Oregon and Washington. Flowering rush (Butomus umbellatus) and fragrant waterlily (Nymphaea odorata) threaten aquatic habitats up and down the Columbia River. Staff from the Center for Lakes & Reservoirs promoted a quick, picture-based key for flowering rush and lookalikes. This presentation was given orally at the Oregon Department of Agriculture's Interagency Noxious Weed Symposium to an audience of natural resource practitioners.

Elucidating The Role Of Astrocytes In Norepinephrine Neuromodulation, Katheryn Bry Lefton

Arts & Sciences Graduate Student Theses and Dissertations

Norepinephrine, a wakefulness-associated neuromodulator, profoundly reshapes neural systems, from synaptic circuits to behavior. Through decades of research, a substantial catalogue on the effects of noradrenergic signaling on behavior and networks has been generated. How norepinephrine remodels synaptic circuitry is widely thought to underlie the larger changes in neural networks and behavior, highlighting the need for a more thorough understanding of the circuit effects of norepinephrine neuromodulation. However, the mechanistic details of the norepinephrine on synapses are still unclear. In recent decades, a large body of work has shown that norepinephrine acts not just on neurons, but on glial cells including …

Sulfhydryl-Containing Compounds And Iron Homeostasis: Mechanisms Of Labile Iron Pool Dysregulation And Consequences For Cellular Health, Steven Lowell Mann Christiansen

Theses and Dissertations

While iron's unique coordination chemistry and redox activity have resulted in its broad incorporation into biological systems, dysfunction in key iron-regulating mechanisms can transform these biochemical advantages into factors that contribute to several pathological conditions. This dissertation explores the complex interactions between iron and sulfhydryl-containing compounds (SCCs), with particular focus on glutathione's (GSH) role in stabilizing iron reactivity juxtaposed with homocysteine's (Hcy) ability to disrupt iron homeostasis and promote disease progression. Through rigorous biochemical analysis and experimentation, we demonstrate that Hcy disrupts iron regulation through several mechanisms: (1) Hcy competitively displaces protective iron-binding molecules in the Labile Iron Pool (LIP), …

The Tomboys Of Cherry Lane, Morgan Flynn

Trade & Scholarly Monographs

You can pack a lot of friendship, feelings, and field hockey into 45 years. Playing on a field hockey team teaches you a lot about yourself and others—including some things you didn’t want to know. Friends who turn out to be as poisonous as toadstools may be the same ones who help you and become a part of who you are. They might even be the friends who will be with you for the rest of your life.

The Rise, Fall, And Resurgence Of Phage Therapy For Urinary Tract Infection, Jacob J Zulk, Kathryn A Patras, Anthony W Maresso

Faculty, Staff and Students Publications

In the face of rising antimicrobial resistance, bacteriophage therapy, also known as phage therapy, is seeing a resurgence as a potential treatment for bacterial infections including urinary tract infection (UTI). Primarily caused by uropathogenic Escherichia coli, the 400 million UTI cases annually are major global healthcare burdens and a primary cause of antibiotic prescriptions in the outpatient setting. Phage therapy has several potential advantages over antibiotics including the ability to disrupt bacterial biofilms and synergize with antimicrobial treatments with minimal side effects or impacts on the microbiota. Phage therapy for UTI treatment has shown generally favorable results in recent …

Ire1Α Silences Dsrna To Prevent Taxane-Induced Pyroptosis In Triple-Negative Breast Cancer, Longyong Xu, Fanglue Peng, Qin Luo, Yao Ding, Fei Yuan, Liting Zheng, Wei He, Sophie S Zhang, Xin Fu, Jin Liu, Ayse Sena Mutlu, Shuyue Wang, Ralf Bernd Nehring, Xingyu Li, Qianzi Tang, Catherine Li, Xiangdong Lv, Lacey E Dobrolecki, Weijie Zhang, Dong Han, Na Zhao, Eric Jaehnig, Jingyi Wang, Weiche Wu, Davis A Graham, Yumei Li, Rui Chen, Weiyi Peng, Yiwen Chen, Andre Catic, Zhibin Zhang, Bing Zhang, Anthony M Mustoe, Albert C Koong, George Miles, Michael T Lewis, Meng C Wang, Susan M Rosenberg, Bert W O'Malley, Thomas F Westbrook, Han Xu, Xiang H-F Zhang, C Kent Osborne, Jin Billy Li, Matthew J Ellis, Mothaffar F Rimawi, Jeffrey M Rosen, Xi Chen

Faculty, Staff and Students Publications

Chemotherapy is often combined with immune checkpoint inhibitor (ICI) to enhance immunotherapy responses. Despite the approval of chemo-immunotherapy in multiple human cancers, many immunologically cold tumors remain unresponsive. The mechanisms determining the immunogenicity of chemotherapy are elusive. Here, we identify the ER stress sensor IRE1α as a critical checkpoint that restricts the immunostimulatory effects of taxane-chemotherapy and prevents the innate immune recognition of immunologically cold triple-negative breast cancer (TNBC). IRE1α RNase silences taxane-induced dsRNA through RIDD (Regulated IRE1-Dependent Decay) to prevent NLRP3 inflammasome–dependent pyroptosis. Inhibition of IRE1α in Trp53−/− TNBC allows taxane to induce extensive dsRNAs that are sensed …

Out-Of-Focus Signal Rejection For In Vivo Po2 Measurements Using Two-Photon Phosphorescence Lifetime Microscopy, Qi Pian, Baoqiang Li, Ikbal Şencan-Eğilmez, Xiaojun Cheng, Jay Dubb, Xinyue Huang, Buyin Fu, Srinivasa Rao Allu, Mohammad Abbas Yaseen, Anna Devor, Sergei A Vinogradov, Sava Sakadžić

2020-Current year OA Pubs

Two-photon phosphorescence lifetime microscopy has been a key tool for studying cerebral oxygenation in mice. However, the accuracy of the partial pressure of oxygen (pO

Small Molecule With Big Impact: Metarrestin Targets The Perinucleolar Compartment In Cancer Metastasis, Vivek Kashyap, Bhuvnesh Sharma, Divya Pandey, Ajay K. Singh, Godwin Peasah-Darkwah, Bhupesh Singh, Kuldeep K. Roy, Murali Yallapu, Subhash Chauhan

School of Medicine Publications

Metarrestin (ML246) is a first-in-class pyrrole-pyrimidine-derived small molecule that selectively targets the perinucleolar compartment (PNC). PNC is a distinct subnuclear structure predominantly found in solid tumor cells. The occurrence of PNC demonstrates a positive correlation with malignancy, serving as an indicator of tumor aggressiveness, progression, and metastasis. Various promising preclinical results have led to the clinical translation of metarrestin into a first-in-human trial. This review aims to summarize (i) the current understanding of the structure and function of PNC and its role in cancer progression and metastasis, (ii) key findings from studies examining the effect of metarrestin on various cancers …

A Ravelin Outwork Uncovered In The Ottoman Fortifications Of The Old City Of ‘Akko, Joppe Gosker

'Atiqot

A small salvage excavation in ‘Akko exposed the remains of a previously unknown ravelin outwork in the counterscarp of the Ottoman-period fortifications—a previously unknown military feature—which cut through earlier Crusader and Hellenistic layers. Ravelins are known components of city fortifications in Europe from the late sixteenth and seventeenth centuries, continuing in use in the nineteenth century. In ‘Akko, it seems that the construction of the ravelin was not completed.

The Late Bronze Age Iii (Iron Age Ia) Gal’On Fortress In The Shephelah, Saar Ganor, Itamar Weissbein

'Atiqot

A previously unknown square building was uncovered at Gal’on in the Judean Shephelah, located between Tel Zayit and Tel Burna. The building was identified as a fortress, similar in plan and dimensions to several other excavated buildings designated “Governors’ Residencies”, and to a few buildings that were defined as “fortresses”—all associated with Egyptian imperial rule in Canaan in Dynasties XIX and XX. The construction of the isolated building at Gal’on may have been related to the reorganization of Canaan under Egyptian rule, possibly in the reign of Ramses III. The building may have been occupied by an Egyptian military force, …

The Elusive Fortifications Of Yafo (Jaffa), Yoav Arbel

'Atiqot

The port city of Yafo was fortified during much of its four millennia of existence. However, today, even its most recent Ottoman walls are dismantled, and only fragmentary parts of its earlier fortifications have been traced despite decades of research and salvage excavations on the mound and its environs. These limited discoveries cannot provide a comprehensive delineation of any pre-Ottoman defensive lines. Nevertheless, new discoveries, the mapping of scattered sites, comparative observations and consultation with historical sources can shed some light on Yafo’s otherwise obscure fortifications through its many phases, as well as on various related issues. Through these we …

A Methodological Framework For The Archaeology Of Conflicts In The Southern Levant, And The Battle Of Hattin (July 3–4, 1187), Rafael Y. Lewis

'Atiqot

This article presents a research framework for the Archaeology of Conflicts/Battlefield Archaeology. This work was motivated by the methodological challenge posed by the very attempt to study material evidence of events of short duration in the landscape. Such events occurred outside the boundaries of conventional archaeological sites, in the landscape, which is generally subject to more frequent changes. The theoretical model is presented through an examination of the Battle of Hattin, a decisive event that took place on July 3–4, 1187, within the days of the Latin Kingdom of Jerusalem (1099–1291). This theoretical construct can be applied to the study …

Were There Jewish Gladiators? A Re-Evaluation Of The Available Archaeological And Textual Evidence, Haggai Olshanetsky

'Atiqot

The participation of Jews in the arena, especially as gladiators, has received relatively little attention. The few articles written on this matter suggest that Jews were both spectators and participants. The present paper relates to all previously suggested indications for the existence of Jewish gladiators and also introduces new ones. Although the possibility that Jewish gladiators were active in the first–fourth centuries CE cannot be ruled out entirely, the evidence remains inconclusive, suggesting that their number was very limited at best. It is also suggested that if Jews had participated in the games, they possibly preferred to fight beasts rather …

Up In Arms: Slingstone Assemblages From The Late Prehistoric Sites Of ‘En Ẓippori And ‘En Esur, Gil Haklay, Hendrik Bron, Dina Shalem, Ianir Milevski, Nimrod Getzov

'Atiqot

This paper presents the slingstone assemblages recovered from two large-scale excavations at the Late Pottery Neolithic/Early Chalcolithic (c. 5800–4500 cal BCE) sites of ‘En Ẓippori and ‘En Esur. Based on contextual, morphometric and other archaeological data, we suggest that during this time, the slings and slingstones were used as weapons. The high number of slingstones found within the settlements, and the traits of systematic production they reflect (formalization, standardization and time-consuming manufacturing), point to an organized production of weapons, interpreted as an escalation in the preparations for war, alluding to the possible existence of local power centers in the region.

Interview With Jim Fields, Coo (Pitt Ohio), Elizabeth Gingerich

The Journal of Values-Based Leadership

No abstract provided.