Digital Commons Network^™

Development Of Sacb-Based Counterselection For Efficient Allelic Exchange In Fusobacterium Nucleatum, Peng Zhou, Bibek G C, Bo Hu, Chenggang Wu

Faculty, Staff and Student Publications

Fusobacterium nucleatum, prevalent in the oral cavity, is significantly linked to overall human health. Our molecular comprehension of its role in oral biofilm formation and its interactions with the host under various pathological circumstances has seen considerable advancements in recent years, primarily due to the development of various genetic tools for DNA manipulation in this bacterium. Of these, counterselection-based unmarked in-frame mutation methods have proved notably effective. Under suitable growth conditions, cells carrying a counterselectable gene die, enabling efficient selection of rare, defined allelic exchange mutants. The sacB gene from Bacillus subtilis, encoding levansucrase, is a widely used …

Composing With Theory: Considerations For Teaching Novice Qualitative Researchers, Janie Copple, Kathryn Roulston

The Qualitative Report

Newcomers to qualitative research are frequently overwhelmed by the range of theories, methodologies, and concepts that qualitative researchers use. This article provides ideas for instructors working with novice qualitative researchers who are beginning to engage with theoretical frameworks used in qualitative research traditions. These practical strategies can assist students navigating challenges commonly encountered in the design and conduct of a first qualitative study. Strategies include developing understandings of disciplinary traditions within which a study will be situated, identifying a phenomenon of interest, defining disciplinary and theoretical terminology, reading deeply, articulating a theoretical perspective, and writing up findings. Through integrating these …

Modulation Of Stemness And Differentiation Regulators By Valproic Acid In Medulloblastoma Neurospheres, Natália Hogetop Freire, Alice Laschuk Herlinger, Julia Vanini, Matheus Dalmolin, Marcelo A C Fernandes, Carolina Nör, Vijay Ramaswamy, Caroline Brunetto De Farias, André Tesainer Brunetto, Algemir Lunardi Brunetto, Lauro José Gregianin, Mariane Da Cunha Jaeger, Michael D Taylor, Rafael Roesler

Faculty, Staff and Students Publications

Changes in epigenetic processes such as histone acetylation are proposed as key events influencing cancer cell function and the initiation and progression of pediatric brain tumors. Valproic acid (VPA) is an antiepileptic drug that acts partially by inhibiting histone deacetylases (HDACs) and could be repurposed as an epigenetic anticancer therapy. Here, we show that VPA reduced medulloblastoma (MB) cell viability and led to cell cycle arrest. These effects were accompanied by enhanced H3K9 histone acetylation (H3K9ac) and decreased expression of the MYC oncogene. VPA impaired the expansion of MB neurospheres enriched in stemness markers and reduced MYC while increasing TP53 …

Microtubules Sequester Acetylated Yap In The Cytoplasm And Inhibit Heart Regeneration, Shijie Liu, Vaibhav Deshmukh, Fansen Meng, Yidan Wang, Yuka Morikawa, Jeffrey D Steimle, Rich Gang Li, Jun Wang, James F Martin

Faculty, Staff and Students Publications

Background: The Hippo pathway effector YAP (Yes-associated protein) plays an essential role in cardiomyocyte proliferation and heart regeneration. In response to physiological changes, YAP moves in and out of the nucleus. The pathophysiological mechanisms regulating YAP subcellular localization after myocardial infarction remain poorly defined.

Methods: We identified YAP acetylation at site K265 by in vitro acetylation followed by mass spectrometry analysis. We used adeno-associated virus to express YAP-containing mutations that either abolished acetylation (YAP-K265R) or mimicked acetylation (YAP-K265Q) and studied how acetylation regulates YAP subcellular localization in mouse hearts. We generated a cell line with YAP-K265R mutation and investigated the …

Advancing De Novo Lipogenesis: Genetic And Metabolic Insights, Sean M Hartig, Mark A Herman

Faculty, Staff and Students Publications

De novo lipogenesis (DNL) is the process whereby cells synthesize fatty acids from acetyl-CoA, contributing to steatosis in fatty liver disease. Two new studies, using genetic mouse models, metabolomics, and pharmacology, identified alternative pathways in DNL and unexpected physiological effects when targeting key enzymes in this pathway.

Yap Overcomes Mechanical Barriers To Induce Mitotic Rounding And Adult Cardiomyocyte Division, Yuka Morikawa, Jong H Kim, Rich Gang Li, Lin Liu, Shijie Liu, Vaibhav Deshmukh, Matthew C Hill, James F Martin

Faculty, Staff and Students Publications

Background: Many specialized cells in adult organs acquire a state of cell cycle arrest and quiescence through unknown mechanisms. Our limited understanding of mammalian cell cycle arrest is derived primarily from cell culture models. Adult mammalian cardiomyocytes, a classic example of cell cycle arrested cells, exit the cell cycle postnatally and remain in an arrested state for the life of the organism. Cardiomyocytes can be induced to re-enter the cell cycle by YAP5SA, an active form of the Hippo signaling pathway effector YAP.

Methods: We performed clonal analyses to determine the cell cycle kinetics of YAP5SA cardiomyocytes. We also performed …

Migration, Trauma, And Tweeback: Perspectives On Russian Mennonite Identity In Elina Penner’S Novel Nachtbeeren, Berit Jany

Journal of Amish and Plain Anabaptist Studies

After the dissolution of the Soviet Union in the 1990s, a group of about 2.5 million Russia Germans immigrated to Germany, benefiting from the German law that recognizes citizenship to ethnic Germans who arrived in the territory as late ethnic Germans resettlers. Including their children, this subset of Germany’s population now numbers about 3.5 million, and about 350.000 Russia Germans have a Mennonite background. Of the 500.000 Plautdietsch-speaking people worldwide, about 200,000 of them live in Germany. In her debut novel, Nachtbeeren, the author Elina Penner illuminates the everyday life of German Mennonites from the Soviet Union and the successor …

Tcas Benchmark, Vol. 32, No. 1, 2018, Chris Meis, Charlene Erwin

North Texas Archeological Society Records

Volume thirty-two and issue one of TCAS Benchmark.

Tcas Benchmark, Vol. 32, No. 1, 2018, Chris Meis, Charlene Erwin

North Texas Archeological Society Records

Volume thirty-two and issue one of TCAS Benchmark.

Tcas Benchmark, Vol. 30, No. 4, 2016, Chris Meis, Catrina Whitley, Mike Shannon

North Texas Archeological Society Records

Volume thirty and issue four of TCAS Benchmark.

Tcas Benchmark, Vol. 29, No. 9, 2015, Chris Meis, Charlene Erwin, Catrina Whitley, Mike Shannon

North Texas Archeological Society Records

Volume twenty-nine and issue nine of TCAS Benchmark. Missing last pages.

Tcas Benchmark, Vol. 29, No. 6, 2015, Chris Meis, Charlene Erwin, Catrina Whitley, Mike Shannon

North Texas Archeological Society Records

Volume twenty-nine and issue six of TCAS Benchmark.

Tcas Benchmark, Vol. 29, No. 2, 2015, Chris Meis, Charlene Erwin, Catrina Whitley, Mike Shannon

North Texas Archeological Society Records

Volume twenty-nine and issue two of TCAS Benchmark.

Tcas Benchmark, Vol. 28, No. 10, 2014, Color Copy, Katrina Nuncio, Charlene Erwin, Jay Hornsby

North Texas Archeological Society Records

Color copy of volume twenty-eight and issue ten of TCAS Benchmark.

Tcas Benchmark, Vol. 28, No. 10, 2014, Katrina Nuncio, Charlene Erwin, Jay Hornsby

North Texas Archeological Society Records

Volume twenty-eight and issue ten of TCAS Benchmark.

Tcas Benchmark, Vol. 28, No. 1, 2014, Katrina Nuncio, Charlene Erwin, Mike Shannon

North Texas Archeological Society Records

Volume twenty-eight and issue one of TCAS Benchmark.

Tcas Benchmark, Vol. 4, No. 11, 2005, Glynn Osburn, Paula Vastine, Jim Blanton

North Texas Archeological Society Records

Volume four and issue eleven of TCAS Benchmark.

Tcas Benchmark, Vol. 4, No. 9, 2005, Glynn Osburn, Paula Vastine

North Texas Archeological Society Records

Volume four and issue nine of TCAS Benchmark.

Tcas Benchmark, Vol. 4, No. 8, 2005, Glynn Osburn, Paula Vastine, Nathan Hays, Deborah Bloys Hardin, Martha Spalding

North Texas Archeological Society Records

Volume four and issue eight of TCAS Benchmark.

Tcas Benchmark, Vol. 4, No. 7, 2005, Glynn Osburn, Paula Vastine, Jesse Todd, Tom Jennings

North Texas Archeological Society Records

Volume four and issue seven of TCAS Benchmark.

Tcas Benchmark, Vol. 4, No. 6, 2005, Glynn Osburn, Paula Vastine

North Texas Archeological Society Records

Volume four and issue six of TCAS Benchmark. Page three is missing.

Tcas Benchmark, Vol. 4, No. 4, 2005, Glynn Osburn, Paula Vastine, Alan Skinner

North Texas Archeological Society Records

Volume four and issue four of TCAS Benchmark.

Wheels Up Jess, Sabrina Hartwell

Night Flight: Online Literary Journal

After a series of unsuccessful dates, Jess feels frustrated with her search for love. With her closest friends' encouragement, she decides to broaden her horizons in her dating options, leading to meeting a captivating woman. Their connection is instant, filled with warmth and adventure. However, as their bond deepens, Jess struggles with feelings of doubt and distance challenges. From a blossoming connection to limitations, Jess ventures on a transformative journey of love, vulnerability, and the beauty of embracing the unexpected. This heartfelt journey leads Jess to move across the country, an unexpected proposal and a new chapter in Jess's life, …

Evaluation Of Green Tea Yoghurt Enriched With Lacticaseibacillus Paracasei E1 Microcapsules On Macrophage M1 Profile In High Fat-Fructose Diet Mice, Esha Ardiansyah, Nur Alfi Maghfirotus Sa’Adah, Rahmi Izati, Belinda Nabiila Al Faizah, Dawama Nur Fadlilah, Septhyanti Aprilia Kavitarna, Mochammad Fitri Atho’Illah, Siti Nur Arifah, Yoga Dwi Jatmiko, Muhaimin Rifa’I

Karbala International Journal of Modern Science

Obesity is caused by an energy imbalance that increases chronic low-grade inflammation, including macrophage cell infiltration. Adipose tissue macrophages are polarized into pro-inflammatory macrophage type 1 (M1), secrete large amounts of pro-inflammatory cytokines, and activate transcription factors. Yoghurt with probiotics is popular at all ages for its health benefits and must be protected by microencapsulation. Green tea (Camellia sinensis L.) fortification provides yoghurt nutrients while improving its functional qualities and bioactivity. This study aimed to evaluate the effect of microencapsulation of Lacticaseibacillus paracasei E1 in green tea yoghurt (GTY) on the profile of M1 macrophages in mice fed a …

Lanthorn, Vol. 59, No. 09, January 6, 2025, Grand Valley State University

Volume 59, August 5, 2024 – April 14, 2025

Lanthorn is Grand Valley State's student newspaper, published from 1968 to the present.

Faculty Senate Agenda January 6, 2025, Utah State University

Faculty Senate & Faculty Senate Executive Committee

• 3:00 Call to Order
• Approval of Minutes
• 3:05 University Business
• 3:20 New Business
• Policy 1001 | Executive Summary | Procedures for Policy
• 3:50 Faculty Senate Business
• State Statute 53B
• 4:00 Information
• EPC Report
• Policy 301 - Employee Position Types
• Policy 368 - Phased Retirement Program
• 4:15 Reports
• Council on Teacher Education Annual Report
• Scholarship Annual Report
• 4:25 Old Business
• Adjourn: 4:30 pm

West Tennessee River Basin Authority (Wtrba) 2024 Annual Report

WTRBA Annual Report

No abstract provided.

Investigating The Thermodynamic Properties Of Fat10 And Fat10ylated Proteins, Aravind R

Theses and Dissertations

Degradation of proteins by the proteasome is crucial in regulating protein levels in the cell. Post-translational modifications, such as ubiquitylation and Fat10ylation, trigger proteasomal degradation of the substrate proteins. While ubiquitylation orchestrates multiple cellular processes, Fat10ylation is primarily involved in the inflammatory response. Unlike ubiquitin, recycled upon substrate degradation, Fat10 is degraded along with its substrate. Although the thermodynamic properties of the substrate are critical for effective proteasomal degradation, they remain poorly understood for the Fat10-proteasome pathway.

Here, we demonstrate that Fat10 exhibits markedly lower thermodynamic stability and faster unfolding kinetics compared to ubiquitin. This is due to the absence …

Role Of Ring-Box E3 Ligases In Runx2 And Sp7 Protein Turnover During Osteoblast Differentiation, Abeera Sikandar

All ETDs from UAB

The bone matrix synthesis by osteoblast during skeletogenesis is regulated by several transcription factors and signaling pathways. Runx2 and Sp7 are master regulators of osteoblast differentiation and bone formation. Deletion of both genes in mice results in the complete failure of ossification. In humans, Sp7 and Runx2 genes mutations cause osteogenesis imperfecta and cleidocranial dysplasia, respectively. The progression of osteoblast differentiation requires a coordinated and temporal expression of Runx2 and Sp7 genes. Posttranslational modifications regulate the cellular levels and function of Runx2 and Sp7 proteins. Ubiquitination controls protein turnover through targeted degradation. The multi-subunit SCF-complex is the largest class of …

Crisprofft: Comprehensive Database Of Crispr/Cas Off-Targets, Grant Wang, Xiaona Liu, Aoqi Wang, Jianguo Wen, Pora Kim, Qianqian Song, Xiaona Liu, Xiaobo Zhou

Faculty, Staff and Student Publications

The CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) programmable nuclease system continues to evolve, with in vivo therapeutic gene editing increasingly applied in clinical settings. However, off-target effects remain a significant challenge, hindering its broader clinical application. To enhance the development of gene-editing therapies and the accuracy of prediction algorithms, we developed CRISPRoffT (https://ccsm.uth.edu/CRISPRoffT/). Users can access a comprehensive repository of off-target regions predicted and validated by a diverse range of technologies across various cell lines, Cas enzyme variants, engineered sgRNAs (single guide RNAs) and CRISPR editing systems. CRISPRoffT integrates results of off-target analysis from 74 studies, encompassing …