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Fragments Within Fragments: The Collective Negotiation Of Exquisite Corpse Drawings, Defry Agatha Ardianta, Yandi Andri Yatmo, Paramita Atmodiwirjo Jan 2025

Fragments Within Fragments: The Collective Negotiation Of Exquisite Corpse Drawings, Defry Agatha Ardianta, Yandi Andri Yatmo, Paramita Atmodiwirjo

Interiority

This paper argues for the critical role of collective drawings as a form of representation that highlights the open process. The open process of collective drawing involves actions performed by multiple actors and actions that are not limited to producing drawings together but also require the expression of ideas. Collective drawing encourages negotiations among fragments of the drawing, which opens up various possibilities of representations within the interiority discourse. Exquisite corpse drawings made by Surrealist artists occupy an influential position as drawings produced via an open process regulated by the internal mechanism, manifesting as a continuous act of drawing. This …

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Decrypting Interiority Of Albert Camus' The Stranger Through Absurdist Architectural Symbolism, Peter Akongfeh Agwu, Angela A. Ajimase, Edward Ugbada Adie, John Ogidi Acha Jan 2025

Decrypting Interiority Of Albert Camus' The Stranger Through Absurdist Architectural Symbolism, Peter Akongfeh Agwu, Angela A. Ajimase, Edward Ugbada Adie, John Ogidi Acha

Interiority

Since its publication in 1942, Camus' The Stranger (L'Étranger in French) has captivated readers and scholars alike, sparking a wealth of critical analysis and interpretation. However, the novel's minimalist descriptions of interior spaces have often been overlooked in academic discourses, making it a perennial subject of further scholarly inquiry. This study seeks to decrypt the interiority of this text by examining the absurdist architectural symbolism and dissecting the spatial elements embedded in its narrative. The aim is to unveil new layers of meaning in the protagonist's internal psyche and existential condition. Through close reading and thematic analysis of relevant passages, …

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Population Variability Across Geographical Ranges: Perspectives And Challenges, Cleber Ten Caten, Tad Dallas Jan 2025

Population Variability Across Geographical Ranges: Perspectives And Challenges, Cleber Ten Caten, Tad Dallas

Faculty Publications

Populations fluctuate over time and across geographical space, and understanding how different factors contribute to population variability is a central goal in population ecology. There is a particular interest in identifying trends of population variability within geographical ranges as population densities of species can fluctuate substantially across geographical space. A common assumption is that populations vary more near species geographical range edges because of unsuitable environments and higher vulnerability to environmental variability in these areas. However, empirical data rarely support this expectation, suggesting that population variability is not related to its position within species geographical ranges. We propose that performance …

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Spatiotemporal Variability And Functional Redundancy Obscure Effects Of Urbanization On Strawberry Pollinators, Kyla D. Schulte, Caleb J. Wilson, Anna Tawril, Mary A. Jamieson Jan 2025

Spatiotemporal Variability And Functional Redundancy Obscure Effects Of Urbanization On Strawberry Pollinators, Kyla D. Schulte, Caleb J. Wilson, Anna Tawril, Mary A. Jamieson

Entomology Faculty Publications

Pollination services across rural–urban gradients may vary as land cover and other environmental attributes differentially influence pollinators. Most studies examining crop pollination, however, have been conducted in rural environments, while research in urban areas has lagged behind despite growing interest in urban agriculture. In this study, we hypothesized that increased urbanization would alter pollinator community composition due to a reduction in natural areas within the surrounding landscape, potentially decreasing pollinator abundance. To evaluate this hypothesis, we conducted a three-year study using strawberries as a focal study crop. We characterized the abundance, diversity, and community composition of bees across 10 farms …

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Dynamics Of Nodes: Adaptation Of Interior Places In A Historic Waterfront Community For Sustainability, Thanapan Laiprakobsup, Narongpon Laiprakobsup Jan 2025

Dynamics Of Nodes: Adaptation Of Interior Places In A Historic Waterfront Community For Sustainability, Thanapan Laiprakobsup, Narongpon Laiprakobsup

Interiority

This study aims to investigate environmental interiority and urban conditions which promote adaptations of nodes as foci of place in the historic, suburban waterfront community. Nodes, existing as strategic places as public activity settings, on the other hand, have been barely examined in terms of the dynamic transition of interior places among urbanisation. The study argues that nodal adaptations taking up dynamic development play crucial functional roles in sustaining a waterfront community in connection with the implementation of place resources and accessible opportunities of new transportation networks. Nodal adaptations lead to the emergence of new functions and place identity in …

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Experience Of Healthcare Workers Who Used Ai Therapy Robot Paro During The Covid-19 Pandemic, Ricco Nancy Julia Jan 2025

Experience Of Healthcare Workers Who Used Ai Therapy Robot Paro During The Covid-19 Pandemic, Ricco Nancy Julia

Walden Dissertations and Doctoral Studies

Healthcare worker burnout and the need for systemic changes in the healthcare field have been researched and discussed for decades. Traditional ways to support these workers’ psychological wellness have not been effective, such that healthcare workers continue to be reluctant to seek help due to stigma and risk of losing their licenses. Researchers have stressed the need for drastic changes in the healthcare field at the administrative level to the resources healthcare workers can use, involving advanced technologies. This initial hermeneutic phenomenology exploratory study was to explore the experiences of healthcare workers who used PARO, a baby seal designed AI …

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2025-01-29 Little Shop Hops: Meowtropolitan, Milan Damjanac Jan 2025

2025-01-29 Little Shop Hops: Meowtropolitan, Milan Damjanac

The Spectator Online

No abstract provided.

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Experience Of Healthcare Workers Who Used Ai Therapy Robot Paro During The Covid-19 Pandemic, Ricco Nancy Julia Jan 2025

Experience Of Healthcare Workers Who Used Ai Therapy Robot Paro During The Covid-19 Pandemic, Ricco Nancy Julia

Walden Dissertations and Doctoral Studies

Healthcare worker burnout and the need for systemic changes in the healthcare field have been researched and discussed for decades. Traditional ways to support these workers’ psychological wellness have not been effective, such that healthcare workers continue to be reluctant to seek help due to stigma and risk of losing their licenses. Researchers have stressed the need for drastic changes in the healthcare field at the administrative level to the resources healthcare workers can use, involving advanced technologies. This initial hermeneutic phenomenology exploratory study was to explore the experiences of healthcare workers who used PARO, a baby seal designed AI …

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Understanding Vaccines: A 3d Animation And Print Materials For Clinical Trial Patients, Shannon Gregory Jan 2025

Understanding Vaccines: A 3d Animation And Print Materials For Clinical Trial Patients, Shannon Gregory

Theses

This project covers the importance of patient education, and the decisions that go into making patient facing materials. The topic of this piece is “Understanding Vaccines” and will explore several key aspects: what vaccines are, how they work, their important role in public health, and how to best communicate with patient audiences. Given the growing challenges in vaccine education, this piece will address common misconceptions and emphasize the importance of clear, accessible communication. The goal of this piece is to educate, but also to encourage individuals to make well-informed decisions regarding vaccination. The artwork created for this thesis was developed …

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Dog Skull Shape Challenges Assumptions Of Performance Specialization From Selective Breeding, Nicholas Hebdon, Alexa Ortega, Alexander Orlove, Nichole Wheeler, Mia Pham, Vivian Nguyen, Justin Gladman, Lindsay D. Waldrop Jan 2025

Dog Skull Shape Challenges Assumptions Of Performance Specialization From Selective Breeding, Nicholas Hebdon, Alexa Ortega, Alexander Orlove, Nichole Wheeler, Mia Pham, Vivian Nguyen, Justin Gladman, Lindsay D. Waldrop

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Through selective breeding, humans have driven exceptional morphological diversity in domestic dogs, creating more than 200 recognized breeds developed for specialized functional tasks such as herding, protection, and hunting. Here, we use three-dimensional reconstructions of dog skulls to ask whether these function-oriented kennel-club groups reflect differences in morphology that correspond to those functions. We analyzed 117 canid skulls, representing 40 domestic dog breeds and 18 wild subspecies, using geometric morphometric techniques and k-means clustering. Results show near-full overlap in kennel-club groups and with natural species, except for companion dogs having extreme snout shape. When categorized by task-specific historic function …

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Evaluation Of Age-Related Spermatozoa Morphological Section Lengths In Gloster Canary Spermatozoa Using Computer Aided Sperm Analysis (Casa) System, Arda Onur Özkök, Burcu Esi̇n, Cumali̇ Kaya Jan 2025

Evaluation Of Age-Related Spermatozoa Morphological Section Lengths In Gloster Canary Spermatozoa Using Computer Aided Sperm Analysis (Casa) System, Arda Onur Özkök, Burcu Esi̇n, Cumali̇ Kaya

The Thai Journal of Veterinary Medicine

The canary is a small passerine bird of the finch family. Canary spermatozoa are also morphologically similar to many other songbird species. In this study, the morphological characteristics of Gloster canary spermatozoa of different age ranges were examined, and the length values of the parts forming the spermatozoa were compared. The study used ten active Gloster canaries between the ages of 3 (Trial-1) and four years (Trial-2). Semen samples from 3-year-old canaries in the study were evaluated, and morphological segment lengths were recorded. In the study, to avoid individual differences and make homogeneous measurements, semen samples were retaken from the …

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Antimicrobial Peptides: Could It Be An Alternative To Antibiotics In Boar Semen Extender?, Krittika Keeratikunakorn, Panida Chanapiwat, Kampon Kaeoket Jan 2025

Antimicrobial Peptides: Could It Be An Alternative To Antibiotics In Boar Semen Extender?, Krittika Keeratikunakorn, Panida Chanapiwat, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

Since antimicrobial resistance is a major global issue, “One Health” approaches such as the use of antimicrobial peptides (AMPs) was developed as an alternative antimicrobial agent which might be used in pig farms to reduce or replace non-rationale antibiotics usage. Although AMPs showed several effects on antimicrobial agents, including against bacteria, fungi, protozoa, and viruses, as well as effects on host immunity, their most notable property is their ability to kill bacteria without causing damage to the host cell. Two major mechanisms of action of AMPs include membrane-active and intracellular-active. During the past decade, many AMPs have been tested for …

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Local Tissue Damage Treatment Of Snake Venom, Suchitra Khunsap Jan 2025

Local Tissue Damage Treatment Of Snake Venom, Suchitra Khunsap

The Thai Journal of Veterinary Medicine

Local tissue damage caused by snake envenomation was a medical problem that caused morbidity and mortality for more than a decade, with no defined treatment guidelines. Nowadays, antivenom is the primary treatment for snakebite, which substantially reduces systemic venom but not significantly reducing local symptoms. We compiled a list of local damage therapies, including antivenom medication, surgery and skin grafting, small molecule medicines, and alternative therapies. Although each was not approved as a standard treatment, it may be a treatment option for tissue damage caused by snake venom. However, antivenoms and most resistant compounds may not be the best strategy …

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The Survey Of The Prevalence Of Equine Gastric Ulcer Syndrome (Egus) In Non-Racing Thoroughbred Horses In Thailand, Teerapol Tum Chinkangsadarn, Sangtawan Tubkleaw, Ananya Rungjarusub, Jitsupa Trangkatarn, Kawinthida Thaworn, Parichart Tesena Jan 2025

The Survey Of The Prevalence Of Equine Gastric Ulcer Syndrome (Egus) In Non-Racing Thoroughbred Horses In Thailand, Teerapol Tum Chinkangsadarn, Sangtawan Tubkleaw, Ananya Rungjarusub, Jitsupa Trangkatarn, Kawinthida Thaworn, Parichart Tesena

The Thai Journal of Veterinary Medicine

This study aimed to determine the prevalence, types, and risk factors of Equine Gastric Ulcer Syndrome (EGUS) in non-racing Thoroughbred horses in Thailand. A total of 56 Thoroughbreds, aged 5 to 22 years (mean age: 12.43 ± 4.65 years), were included. Before sedation, a comprehensive history and physical examination were conducted. Gastroscopy was performed to identify gastric ulcers, using the Equine Gastric Ulcer Council 0–4 scoring system for non-glandular ulcers, while glandular ulcers were assessed as present or absent. Data on breed, age, sex, activity status, behavior, appetite, and medical rehistory were collected. The overall prevalence of gastric ulcers was …

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'The Tale Of Peter Rabbit'; 'Snowy Day'; & 'Bubba', Jenni Martinez, Erin Robinson, Jacob Crock Jan 2025

'The Tale Of Peter Rabbit'; 'Snowy Day'; & 'Bubba', Jenni Martinez, Erin Robinson, Jacob Crock

.RAW Journal of Art and Design

No abstract provided.

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Full Issue, Erin Robinson, Michaela Velleno Jan 2025

Full Issue, Erin Robinson, Michaela Velleno

.RAW Journal of Art and Design

No abstract provided.

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Metabolic Effects Of R-1,3-Butanediol (Ketone-Iq) During Aerobic And Anaerobic Exercise Bouts, Mathew C. Soto, Andrew J. Jakiel, Latt Mansor, Jenica D. Alvarez, Ashley M. Bruce, Blake Podsen, Sabrina R. Fordham, Parker N. Hyde Jan 2025

Metabolic Effects Of R-1,3-Butanediol (Ketone-Iq) During Aerobic And Anaerobic Exercise Bouts, Mathew C. Soto, Andrew J. Jakiel, Latt Mansor, Jenica D. Alvarez, Ashley M. Bruce, Blake Podsen, Sabrina R. Fordham, Parker N. Hyde

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 6: Issue 1, Article 1, 2025. BACKGROUND: Current evidence demonstrates the efficacy of low carbohydrate/high fat ketogenic diets as it related to exercise. Exogenous ketone supplements have made it possible to ingest ketone molecules, elevating blood b-hydroxybutyrate (BHB) despite consuming carbohydrates (CHO). The purpose of this investigation was to determine both metabolic and performance effects of R-1,3-butanediol (BDO). METHODS: A randomized repeated measures placebo-controlled design was used to compare BDO and placebo (PLA). Each phase of testing utilized two primary testing days: 1) a dose exposure day designed to determine blood metabolite changes …

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No. 33: Living Through The Covid-19 Pandemic As A Refugee In Secondary Cities In Canada: The Intersectionality Of Immobility, Gender And Food Insecurity, Zhenzhong Si, Zack Ahmed, Sujata Ramachandran, Neil Arya, Jonathan Crush Jan 2025

No. 33: Living Through The Covid-19 Pandemic As A Refugee In Secondary Cities In Canada: The Intersectionality Of Immobility, Gender And Food Insecurity, Zhenzhong Si, Zack Ahmed, Sujata Ramachandran, Neil Arya, Jonathan Crush

Migration and Food Security (MiFOOD) Network

The COVID-19 pandemic exposed and exacerbated the vulnerabilities of migrants and refugees in secondary cities in Canada, where the restrictive food environment and limited resources heightened challenges related to food security. This study investigates how the intersectionality of immobility, gender, and food insecurity shaped the lived experiences of recently resettled Syrian, Somali and Afghanistan refugees in the Waterloo Region, Canada, during the pandemic. The mixed methods research approach integrates survey and in-depth interview data to examine refugees’ motivations for migration, economic conditions, challenges in accessing culturally appropriate food, and the impact of gender roles. Findings reveal that structural barriers within …

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Resiliency’S Correlations To Autonomy, Competence, Relatedness, And Meaning In Life For The Trucking Industry During Covid-19, Rhonda Renee Wood Jan 2025

Resiliency’S Correlations To Autonomy, Competence, Relatedness, And Meaning In Life For The Trucking Industry During Covid-19, Rhonda Renee Wood

Walden Dissertations and Doctoral Studies

COVID-19 depleted multiple subsystems, took human lives, impaired mental health, and induced economic devastation. The trucking occupation was and continues to be impacted by adverse working conditions, and the pandemic’s limitations intensified the stress that truckers experience. COVID-19 resilience for trucking employees may stratify to other supply chains. Therefore, the quantitative cross-sectional study aimed to assess the research questions of how meaning in life, autonomy, competence, and relatedness correlated to each other and preceded resilience. The theoretical framework included Viktor Frankl’s meaning in life and Ryan and Deci’s self-determination theories. Connect Cloud Research was the platform for data retrieval from …

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Behavioral And Neuroactivational Correlates Of Psychosocial Stress-Induced Cocaine Seeking In The Rat, Nicole Hinds Jan 2025

Behavioral And Neuroactivational Correlates Of Psychosocial Stress-Induced Cocaine Seeking In The Rat, Nicole Hinds

Theses and Dissertations

Abstract Nicole Maria Hinds BEHAVIORAL AND NEUROACTIVATIONAL CORRELATES OF PSYCHOSOCIAL STRESS-INDUCED COCAINE SEEKING IN THE RAT 2024 Molecular Cell Biology & Neuroscience Doctor of Philosophy Cocaine Use Disorder is characterized by a high risk for relapse that can persist over extended periods of abstinence. Psychosocial stressors are well-established to promote drug craving and relapse in humans but have rarely been employed in preclinical relapse models. Consequently, the underlying neural circuitry by which these stressors drive cocaine seeking has not yet been thoroughly explored. This project aimed to identify brain regions recruited during psychosocial stress-induced cocaine seeking in rats, with a …

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Spam, Solidarity, And Roving Pickets: The Hormel P-9 Strike Of 1985-86 In Iowa, Scott Pate Jan 2025

Spam, Solidarity, And Roving Pickets: The Hormel P-9 Strike Of 1985-86 In Iowa, Scott Pate

History Theses, Dissertations, and Student Creative Activity

In the 1980s, the labor movement reached a crossroads with economic changes and corporate restructuring challenging the effectiveness of New Deal labor unions. Meatpacking proved one of the hardest-hit industries, with laborers accepting concessions that jeopardized their quality of life. The Hormel P-9 Strike of 1985-86 exemplifies the rank-and-file’s struggle against neoliberalism and status quo business unionism. Rachleff’s Hard-Pressed in the Heartland argues for the importance of the P-9 as a social movement, placing the conflict within the context of the American labor movement, while Stromquist and Warren indicate the importance of Iowa labor when evaluating labor issues in the …

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Animal Science News (January 28, 2025), Department Of Animal Science Jan 2025

Animal Science News (January 28, 2025), Department Of Animal Science

Animal Science Newsletters

No abstract provided.

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Exploring The Role Of Cyclin D1 In Regulating Thermogenic Program In Brown Adipose Tissue, Chengrui Qiu Jan 2025

Exploring The Role Of Cyclin D1 In Regulating Thermogenic Program In Brown Adipose Tissue, Chengrui Qiu

Biomedical Sciences ETDs

Abstract Adipose tissue plays a multifaceted role in regulating metabolic health and contributing to the pathophysiology of various diseases. Brown Adipose Tissue (BAT), a vital organ that senses different environmental cues such as cold stress and diet and then undergoes non-shivering thermogenesis in mammalian animals, enhancing energy expenditure to combat obesity. In our recent published study, we found that the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway exhibits doubleedge effects in regulating thermogenic function, with opposite role in BAT and white adipose tissue (WAT). To further investigate the molecular mechanisms underlying the dual role of mTORC1 in thermogenic …

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Health And Safety Policies For Non-Profit Secondary School Boarding Facilities In Sub-Saharan Central Africa, Jonathon W. Holloway Jan 2025

Health And Safety Policies For Non-Profit Secondary School Boarding Facilities In Sub-Saharan Central Africa, Jonathon W. Holloway

Walden Dissertations and Doctoral Studies

This quantitative professional administrative study was conducted to identify a gap in policies that do not fully address or provide fact-based connections to the physiological health and safety needs of secondary grade (S1 to S6) school students to grow and learn while housed in on-campus boarding facilities located in the central sub-Saharan region of Africa. The practice-focused question was: What are the most effective policies for the health and safety of secondary grade students within the boarding facility of a secondary school in the sub-Saharan central African region? Secondary analysis, utilizing ANOVA testing, was conducted on data sets developed by …

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Overcoming Cd226-Related Immune Evasion In Acute Myeloid Leukemia With Cd38 Car-Engineered Nk Cells, Luciana Melo Garcia, Achintyan Gangadharan, Pinaki Banerjee, Ye Li, Andy G X Zeng, Hind Rafei, Paul Lin, Bijender Kumar, Sunil Acharya, May Daher, Luis Muniz-Feliciano, Gary M Deyter, Gabriel Dominguez, Jeong Min Park, Francia Reyes Silva, Ana Karen Nunez Cortes, Rafet Basar, Nadima Uprety, Mayra Shanley, Mecit Kaplan, Enli Liu, Elizabeth J Shpall, Katayoun Rezvani Jan 2025

Overcoming Cd226-Related Immune Evasion In Acute Myeloid Leukemia With Cd38 Car-Engineered Nk Cells, Luciana Melo Garcia, Achintyan Gangadharan, Pinaki Banerjee, Ye Li, Andy G X Zeng, Hind Rafei, Paul Lin, Bijender Kumar, Sunil Acharya, May Daher, Luis Muniz-Feliciano, Gary M Deyter, Gabriel Dominguez, Jeong Min Park, Francia Reyes Silva, Ana Karen Nunez Cortes, Rafet Basar, Nadima Uprety, Mayra Shanley, Mecit Kaplan, Enli Liu, Elizabeth J Shpall, Katayoun Rezvani

Faculty, Staff and Student Publications

CD226 plays a vital role in natural killer (NK) cell cytotoxicity, interacting with its ligands CD112 and CD155 to initiate immune synapse formation, primarily through leukocyte function-associated-1 (LFA-1). Our study examined the role of CD226 in NK cell surveillance of acute myeloid leukemia (AML). NK cells in patients with AML had lower expression of CD226. CRISPR-Cas9 deletion of CD226 led to reduced LFA-1 recruitment, poor synapse formation, and decreased NK cell anti-leukemic activity. Engineering NK cells to express a chimeric antigen receptor targeting the AML antigen CD38 (CAR38) could overcome the need for CD226 to establish strong immune synapses. LFA-1 …

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Health And Safety Policies For Non-Profit Secondary School Boarding Facilities In Sub-Saharan Central Africa, Jonathon W. Holloway Jan 2025

Health And Safety Policies For Non-Profit Secondary School Boarding Facilities In Sub-Saharan Central Africa, Jonathon W. Holloway

Walden Dissertations and Doctoral Studies

This quantitative professional administrative study was conducted to identify a gap in policies that do not fully address or provide fact-based connections to the physiological health and safety needs of secondary grade (S1 to S6) school students to grow and learn while housed in on-campus boarding facilities located in the central sub-Saharan region of Africa. The practice-focused question was: What are the most effective policies for the health and safety of secondary grade students within the boarding facility of a secondary school in the sub-Saharan central African region? Secondary analysis, utilizing ANOVA testing, was conducted on data sets developed by …

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Resiliency’S Correlations To Autonomy, Competence, Relatedness, And Meaning In Life For The Trucking Industry During Covid-19, Rhonda Renee Wood Jan 2025

Resiliency’S Correlations To Autonomy, Competence, Relatedness, And Meaning In Life For The Trucking Industry During Covid-19, Rhonda Renee Wood

Walden Dissertations and Doctoral Studies

COVID-19 depleted multiple subsystems, took human lives, impaired mental health, and induced economic devastation. The trucking occupation was and continues to be impacted by adverse working conditions, and the pandemic’s limitations intensified the stress that truckers experience. COVID-19 resilience for trucking employees may stratify to other supply chains. Therefore, the quantitative cross-sectional study aimed to assess the research questions of how meaning in life, autonomy, competence, and relatedness correlated to each other and preceded resilience. The theoretical framework included Viktor Frankl’s meaning in life and Ryan and Deci’s self-determination theories. Connect Cloud Research was the platform for data retrieval from …

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Erk Activation Dynamics In Maturing Oocyte Controls Embryonic Nuclear Divisions In Caenorhabditis Elegans, Han Bit Baek, Debabrata Das, Shin-Yu Chen, Hongyuan Li, Swathi Arur Jan 2025

Erk Activation Dynamics In Maturing Oocyte Controls Embryonic Nuclear Divisions In Caenorhabditis Elegans, Han Bit Baek, Debabrata Das, Shin-Yu Chen, Hongyuan Li, Swathi Arur

Faculty, Staff and Student Publications

ERK activity oscillates between sustained activation during oocyte formation and transient inactivation during oocyte maturation, fertilization, and early embryogenesis. Consequences of ectopic ERK activity upon oocyte maturation and in early embryogenesis are unknown. We show, in Caenorhabditis elegans, that ectopic ERK activity upon oocyte maturation (metaphase I oocytes) results in embryos with abnormalities in nuclear divisions leading to embryonic death. We uncover that ERK directly phosphorylates Polo-like kinase I (PLK-1), on Serine 404, to inhibit nuclear envelope breakdown (NEBD) in early embryogenesis. The RAS/ERK/PLK-1 pathway poisons zygotic NEBD and inhibits the merging of parental genomes, underlining the importance of turning …

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Sex-Specific Astrocyte Regulation Of Spinal Motor Circuits By Nkx61, Navish A Bosquez Huerta, Zhung-Fu Lee, Eun-Ah Christine Song, Junsung Woo, Yi-Ting Cheng, Debosmita Sardar, Ozlem Sert, Ehson Maleki, Kwanha Yu, Ekin Su Akdemir, Kaitlyn Sanchez, Juyeon Jo, Matthew N Rasband, Hyun Kyoung Lee, Akdes Serin Harmanci, Benjamin Deneen Jan 2025

Sex-Specific Astrocyte Regulation Of Spinal Motor Circuits By Nkx61, Navish A Bosquez Huerta, Zhung-Fu Lee, Eun-Ah Christine Song, Junsung Woo, Yi-Ting Cheng, Debosmita Sardar, Ozlem Sert, Ehson Maleki, Kwanha Yu, Ekin Su Akdemir, Kaitlyn Sanchez, Juyeon Jo, Matthew N Rasband, Hyun Kyoung Lee, Akdes Serin Harmanci, Benjamin Deneen

Faculty, Staff and Students Publications

Astrocytes exhibit diverse cellular and molecular properties across the central nervous system (CNS). Recent studies identified region-specific transcription factors (TF) that oversee these diverse properties; how sex differences intersect with region-specific transcriptional programs to regulate astrocyte function is unknown. Here, we show that the TF Nkx6.1 is specifically expressed in ventral astrocytes of the spinal cord and that its deletion results in sex-specific effects on astrocyte morphology. Astrocytes from males exhibit enhanced morphological complexity, accompanied by increased motor function and cholinergic synapses. In contrast, female astrocytes exhibit reduced complexity and no changes in motor function. Mechanistically, we found that Nkx6.1 …

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The Adiponectin-Pparγ Axis In Hepatic Stellate Cells Regulates Liver Fibrosis, Shangang Zhao, Qingzhang Zhu, Wang-Hsin Lee, Jan-Bernd Funcke, Zhuzhen Zhang, May-Yun Wang, Qian Lin, Bianca Field, Xue-Nan Sun, Guannan Li, Mbolle Ekane, Toshiharu Onodera, Na Li, Yi Zhu, Christine M Kusminski, Terry D Hinds, Philipp E Scherer Jan 2025

The Adiponectin-Pparγ Axis In Hepatic Stellate Cells Regulates Liver Fibrosis, Shangang Zhao, Qingzhang Zhu, Wang-Hsin Lee, Jan-Bernd Funcke, Zhuzhen Zhang, May-Yun Wang, Qian Lin, Bianca Field, Xue-Nan Sun, Guannan Li, Mbolle Ekane, Toshiharu Onodera, Na Li, Yi Zhu, Christine M Kusminski, Terry D Hinds, Philipp E Scherer

Faculty, Staff and Students Publications

Hepatic stellate cells (HSCs) are key drivers of local fibrosis. Adiponectin, conventionally thought of as an adipokine, is also expressed in quiescent HSCs. However, the impact of its local expression on the progression of liver fibrosis remains unclear. We recently generated a transgenic mouse line (Lrat-rtTA) that expresses the doxycycline-responsive transcriptional activator rtTA under the control of the HSC-specific lecithin retinol acyltransferase (Lrat) promoter, which enables us to specifically and inducibly overexpress or eliminate genes in these cells. The inducible elimination of HSCs protects mice from methionine/choline-deficient (MCD) diet-induced liver fibrosis, confirming their causal involvement in fibrosis development. We generated …

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