Digital Commons Network^™

Engineered Immunomodulatory Extracellular Vesicles From Epithelial Cells With The Capacity For Stimulation Of Innate And Adaptive Immunity In Cancer And Autoimmunity, Xin Luo, Fernanda G Kugeratski, Dara P Dowlatshahi, Hikaru Sugimoto, Kent A Arian, Yibo Fan, Li Huang, Danielle Wills, Sergio Lilla, Kelly Hodge, Sara R Zanivan, Valerie S Lebleu, Kathleen M Mcandrews, Raghu Kalluri

Faculty, Staff and Student Publications

Extracellular vesicles (EVs) are generated by all cells. Systemic administration of allogenic EVs derived from epithelial and mesenchymal cells have been shown to be safe, despite carrying an array of functional molecules, including thousands of proteins. To address whether epithelial cells derived EVs can be modified to acquire the capacity to induce immune response, we engineered 293T EVs to harbor the immunomodulatory molecules CD80, OX40L and PD-L1. We demonstrated abundant levels of these proteins on the engineered cells and EVs. Functionally, the engineered EVs efficiently elicited positive and negative co-stimulation of human and murine T cells. In the setting of …

Complete Genome Sequence Of A Penicillin-Resistant Fusobacterium Necrophorum Subsp Funduliforme Isolate From A Tonsillitis Patient, Bibek G C, Anders Jensen, Chenggang Wu

Faculty, Staff and Student Publications

We report the complete genome sequence of a penicillin-resistant Fusobacterium necrophorum subsp. funduliforme isolate, AJ79, from a tonsillitis patient. The AJ79 genome consists of a chromosome (2,440,359 bp) and plasmid (9,887 bp), providing insights into the genetic basis of penicillin resistance in F. necrophorum and its implications for treating tonsillitis.

Restore The Shore: A Guide To Lakeshore Revegetation In The Great Basin, Jes V. Braun, Rae Robinson, Annie L. Henry, Karin M. Kettenring

All Current Publications

Lakeshore vegetation supports many valuable aspects of lake ecosystems: foraging areas for birds, rearing grounds for fish, nutrient cycling, carbon storage, and aesthetic, cultural, and spiritual values. Yet, the vegetation of many lakeshores has been lost or heavily impacted by humans. What can be done to restore lakeshore vegetation to ensure that lakes remain healthy for future generations? In this guide, we summarize best practices for lakeshore revegetation (i.e., the process of actively reintroducing vegetation, usually native species, to a restoration site through seeding and planting) in the Great Basin.

Phosphorylation Of Cryab Induces A Condensatopathy To Worsen Post-Myocardial Infarction Left Ventricular Remodeling, Moydul Islam, David R. Rawnsley, Xiucui Ma, Walter Navid, Chen Zhao, Xumin Guan, Layla Foroughi, John T. Murphy, Honora Navid, Carla J. Weinheimer, Attila Kovacs, Jessica Nigro, Aaradhya Diwan, Ryan P. Chang, Minu Kumari, Martin E. Young, Babak Razani, Kenneth B. Margulies, Mahmoud Abdellatif, Simon Sedej, Ali Javaheri, Douglas F. Covey, Kartik Mani, Abhinav Diwan

2020-Current year OA Pubs

Protein aggregates are emerging therapeutic targets in rare monogenic causes of cardiomyopathy and amyloid heart disease, but their role in more prevalent heart-failure syndromes remains mechanistically unexamined. We observed mislocalization of desmin and sarcomeric proteins to aggregates in human myocardium with ischemic cardiomyopathy and in mouse hearts with post-myocardial infarction ventricular remodeling, mimicking findings of autosomal-dominant cardiomyopathy induced by the R120G mutation in the cognate chaperone protein CRYAB. In both syndromes, we demonstrate increased partitioning of CRYAB phosphorylated on serine 59 to NP40-insoluble aggregate-rich biochemical fraction. While CRYAB undergoes phase separation to form condensates, the phosphomimetic mutation of serine 59 …

Updated Sc 4-H Presents Packet, Extension

UNH Cooperative Extension

No abstract provided.

Intensive Lifestyle Intervention, Cardiac Biomarkers, And Cardiovascular Outcomes In Diabetes: Look Ahead Cardiac Biomarker Ancillary Study, Kershaw V Patel, Zainali Chunawala, Subodh Verma, Matthew W Segar, Katelyn R Garcia, Chiadi E Ndumele, Thomas J Wang, James L Januzzi, Antoni Bayes-Genis, Javed Butler, Carolyn S P Lam, Christie M Ballantyne, James A De Lemos, Alain G Bertoni, Mark Espeland, Ambarish Pandey

Faculty, Staff and Students Publications

Background: N-terminal pro-B-type natriuretic peptide (NT-proBNP) and high-sensitivity cardiac troponin T (hs-cTnT) are associated with cardiovascular outcomes and are recommended for measurement in type 2 diabetes (T2D). However, the effects of an intensive lifestyle intervention (ILI) targeting weight loss on cardiac biomarkers and the prognostic association of changes in these biomarkers with risk of adverse cardiovascular outcomes in T2D are not well-established.

Objectives: This study sought to evaluate the effects of an ILI on cardiac biomarkers and the association of changes in cardiac biomarkers with risk of cardiovascular outcomes in T2D.

Methods: Participants of the Look AHEAD (Action for Health …

Mbnl Overexpression Rescues Cardiac Phenotypes In A Myotonic Dystrophy Type 1 Heart Mouse Model, Rong-Chi Hu, Yi Zhang, Larissa Nitschke, Sara J Johnson, Ayrea E Hurley, William R Lagor, Zheng Xia, Thomas A Cooper

Faculty, Staff and Students Publications

Myotonic dystrophy type 1 (DM1) is an autosomal dominant disease caused by a CTG repeat expansion in the dystrophia myotonica protein kinase (DMPK) gene. The expanded CUG repeat RNA (CUGexp RNA) transcribed from the mutant allele sequesters the muscleblind-like (MBNL) family of RNA-binding proteins, causing their loss of function and disrupting regulated pre-mRNA processing. We used a DM1 heart mouse model that inducibly expresses CUGexp RNA to test the contribution of MBNL loss to DM1 cardiac abnormalities and explored MBNL restoration as a potential therapy. AAV9-mediated overexpression of MBNL1 and/or MBNL2 significantly rescued DM1 cardiac phenotypes including conduction delays, contractile …

The Tie2 Antagonist Rebastinib Reduces Ovarian Cancer Growth In A Syngeneic Murine Model, Vijayalaxmi G Gupta, Katherine F Roby, Harsh B Pathak, Andrew K Godwin, Sumedha Gunewardena, Dineo Khabele

2020-Current year OA Pubs

BACKGROUND: The receptor tyrosine kinase TIE2 and its ligands, angiopoietins (ANGPTs), promote angiogenesis. In addition to expression on vascular endothelial cells, TIE2 is expressed on M2-like pro-tumorigenic macrophages. Thus, the TIE2 inhibitor rebastinib was developed as a potential therapy to address multiple cancers. The objective of this study was to determine the effects of rebastinib alone and combined with chemotherapy in a syngeneic murine model of ovarian cancer.

METHODS: Female C57Bl6J mice were intraperitoneally injected with syngeneic ID8 ovarian cancer cells. Once tumors were established, mice were untreated (control) or treated with rebastinib, carboplatin plus paclitaxel (chemotherapy), or rebastinib plus …

Formulation, Characterization, And In Vitro–In Vivo Evaluation Of Self Microemulsifying Drug Delivery System Of Ebastine By Spray Drying Technology Using Solid Carriers, Vijay Rajaram Mahajan, Ganesh Dinkarrao Basarkar

The Thai Journal of Pharmaceutical Sciences

Aim: The present work was aimed at formulating novel liquid self microemulsifying oil formulation and its solid form by spray drying method for improving the solubility of Ebastine, an antiallergic drug and BCS class II drug, evaluating its in vitro and in vivo potential. Results: The self emulsifying oil formulation was comprised of Rice Bran Oil, Tween 40, Capryol 90. Pseudoternary phase diagrams were used to evaluate the micro emulsification existence area, and the release rate of Ebastine was investigated using an in vitro dissolution test. Accelerated stability testing of the formulation was also assessed at 400C/75% RH for 3 …

Antidepressant-Like Effect Of Minocycline In Mice Forced Swimming Test: Minor Involvement Of The Noradrenergic System, Azadeh Mesripour, Mohsen Kavianpour, Valiollah Hajhashemi

The Thai Journal of Pharmaceutical Sciences

Introduction: Minocycline is a tetracycline antibiotic drug that is receiving attention as an alternative for the treatment of depression. Several mechanisms have been proposed for this effect. The current study evaluated the involvement of the noradrenergic system in the antidepressant-like effect of minocycline. Materials and Methods: The total immobility time was evaluated as an index of depression in mice forced swimming test (FST). Minocycline (40 mg/kg) was injected for 3 days. Imipramine (5 mg/kg, a tricyclic antidepressant), prazosin (1 mg/kg, an α1-adrenoceptor antagonist), yohimbine (1 mg/kg, an α2-adrenoceptor antagonist), and propranolol (2 mg/kg, a β-adrenoceptor antagonist) were injected 30 min …

Use Of Synthetic Water-Soluble Kcc2 To Modulate Chloride Homeostasis In Cultured Dopamine-Neuron-Like Cells, Mason Terry, Andrew Payne, Dario Mizrachi

Annual Research Symposium

No abstract provided.

Religious Moral Suasion And Material Support For The Environmental Justice Movement, Sarah Elizabeth Nahar

International Journal on Responsibility

The Environmental Justice (EJ) movement in the United States is comprised of diverse groups of people with a variety of environmental grievances and interests coming together to obtain equal distribution of pollution burdens across communities, reduce environmental hazards, and ensure fair enforcement of laws and policies meant to safeguard the environment for all. The 17 Principles of Environmental Justice developed in 1991 at the First National People of Color Environmental Leadership Summit remains a touchstone document today. In addition to Indigenous and Black leadership, religious communities also made the Summit possible. Religious adherents and institutions have always influenced the movement …

Complete Issue - Mend (Spring 2025), Mend Journal

Mend

No abstract provided.

Running, Sergey Medvedev

Mend

No abstract provided.

The Fall, Dymitri Hubert Haraszewski

Mend

No abstract provided.

Falling, Michael Shane Hale

Mend

No abstract provided.

Pavlov's Dogs, Gary K. Farlow

Mend

No abstract provided.

Section Ii: Listen Up!, Marion Rodriguez, Patrick W. Berry

Mend

No abstract provided.

Time And Prison: Are They Mutually Exclusive?, Marvin Wade

Mend

No abstract provided.

Characterization Of 1,8-Cineole (Eucalyptol) From Myrtle And Its Potential Antibacterial And Antioxidant Activities*, Humera Khan

Karbala International Journal of Modern Science

1,8-Cineole is a naturally occurring chemical molecule predominantly found in fragrant plants, particularly Myrtle. Its aroma is distinctive and has been the subject of numerous investigations due to its various biological actions. This study examines the characterization of 1,8-Cineole derived from Myrtle and investigates its antibacterial and antioxidant properties. This study seeks to compare 1,8-Cineole with antibiotics like Amoxicillin and Tetracycline, and moreover, to investigate its antioxidant capabilities against diverse bacterial strains (both Gram-positive and Gram-negative). 1,8-Cineole exhibits the most effective antibacterial properties, demonstrating an inhibition zone of 12.00 mm against Staphylococcus aureus and 10.00 mm against Pseudomonas aeruginosa. …

Impact Of Resistance Training On Bone During 40% Caloric Restriction In Growing Female Rats, Ken D. Sumida, Daniel L. Smithers, Aaron Gerston, Kim A. Lagerborg, S. Victoria Jaque, Fred Caporaso

Food Science Faculty Articles and Research

There is a growing trend in the use of severe caloric restrictive diets among normal weight young females that can jeopardize bone health. Using an animal model, the purpose of this study was to determine whether resistance training (RT) could maintain bone health during a 6-week severe caloric restrictive (CR) diet in growing female rats. Twenty-four female rats (~ 8 weeks old) were randomly divided into the following groups: sedentary rats fed a normal diet (N = 8), sedentary rats fed a 40% CR diet (D = 8), and an RT group fed a 40% CR diet (DT …

Curricular Changes Proposed For 2025-2027 Morris Catalog, Curriculum Committee

Curriculum Committee Reports

No abstract provided.

The Joy Of Embodied Learning, Leslie Bayers

Academic Affairs Faculty and Staff Books and Book Chapters

The science of embodied cognition has established that thinking involves dynamic exchanges between the body and the brain and webs of interaction between humans and their environments. While learning draws on these connected ecosystems, persistent notions of the brain as separate from and hierarchically managing the body—an enduring legacy of Cartesian dualism—perpetuate static teaching and learning mindsets. Disregard for the body’s role in learning fuels disengagement, isolation, and inequity. This chapter weaves key findings from embodied learning research together with scenes from the author’s own academic journey to illustrate how leveraging brain–body connections can foster educational well-being. The author shares …

The Rider (2025-02-10), Rebeca Salinas

The Rider Archives

Vol. 10, No. 18

Student Senate Meeting Minutes February 10, 2025, Student Senate-Winona State University

Student Senate Meeting Minutes

This document is the official Winona State University Student Senate Minutes for February 10, 2025.

Locational Data And The Public Interest, William A. Herbert, Micahel Goodchild, Richard Appelbaum, Jeremy Crampton, Gary Langham, Krzysztof Janowicz, Mei-Po Kwan, Katina Michael, Lisa Schamess

Publications and Research

This article presents a paper developed by the AAG Organizing Committee on Locational Information and the Public Interest through a summit held in Santa Barbara, California in June 2022. The summit resulted in goals and ideas for addressing the issues that arise from the present environment for geodata, whereby public, private, and third-sector entities can tap into publicly available locational information with relatively little regulation on its access or use. The Committee articulates four goals: (1) develop a research agenda extending across disciplines, (2) outline educational resources and strategies to guide ethical practice, (3) devise a pathway to increase public …

Characteristics And Outcomes Of Adults Hospitalized With Respiratory Syncytial Virus, Brian S. Shaw, Cara Slaton, Ian P. Murphy, Michael Mcalister, Daniel Egan, Kwabena Ayesu

The University of Louisville Journal of Respiratory Infections

Background: Respiratory syncytial virus (RSV) is a negative sense single-stranded RNA virus (ssRNA) that primarily causes respiratory infections in both children and older adults. We examined clinical characteristics and outcomes of hospitalized adult patients who tested positive for RSV.

Methods: This was a multicenter, retrospective, descriptive study that examined adult patients admitted with a positive RSV test over two years across eight hospitals in Orlando, Florida.

Results: A total of 120 patients were included, of which 88 (73%) were 60 years old and older. Overall, patients with RSV infection were caucasian (62%) with a median age of 70 years and …

A Critical Appraisal Of Poverty In The Context Of Nature-Based Communities: Selected Cases From The Philippines, Carla Edith Gonzales Jimena

Dissertations and Theses

The Philippines is considered one of the wealthiest countries in both Southeast Asia and the world when measured by biodiversity, mineral resources and natural resources. It is also one of the most highly mineralized countries on earth. However, despite these natural endowments, when measured by established economic and human development indices the Philippines remains poor. Using critical perspectives derived from Marxist tradition, William Chambliss' theory of law and its dialectical nature, Immanuel Wallerstein's World Systems Theory, and Fred Block's structural mechanism of the state, this study aimed to critically appraise the persistence of poverty in nature-based communities in the Philippines. …

A Deep Learning-Based System For Automatic Detection Of Emesis With High Accuracy In Suncus Murinus, Zengbing Lu, Yimeng Qiao, Xiaofei Huang, Dexuan Cui, Julia Y H Liu, Man Piu Ngan, Luping Liu, Zhixin Huang, Zi-Tong Li, Lingqing Yang, Aleena Khalid, Yingyi Deng, Sze Wa Chan, Longlong Tu, John A Rudd

Children’s Nutrition Research Center Staff Publications

Quantifying emesis in Suncus murinus (S. murinus) has traditionally relied on direct observation or reviewing recorded behaviour, which are laborious, time-consuming processes that are susceptible to operator error. With rapid advancements in deep learning, automated animal behaviour quantification tools with high accuracy have emerged. In this study, we pioneere the use of both three-dimensional convolutional neural networks and self-attention mechanisms to develop the Automatic Emesis Detection (AED) tool for the quantification of emesis in S. murinus, achieving an overall accuracy of 98.92%. Specifically, we use motion-induced emesis videos as training datasets, with validation results demonstrating an accuracy of 99.42% for …

Editorial: Review Of Hyperbaric Therapy & Hyperbaric Oxygen Therapy In The Treatment Of Neurological Disorders According To Dose Of Pressure And Hyperoxia, Paul G. Harch, George Mychaskiw, John H. Zhang, Dominic P. D'Agostino, Keith Van Meter, Enrico M. Camporesi

School of Medicine Faculty Publications

No abstract provided.