Digital Commons Network^™

Studying The Mechanism For Ricin-Induced Growth Suppression, Daniel Judge

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study focuses on ribosome-inactivating proteins (RIPs), a class of potent toxins that includes ricin, α-sarcin, and shiga toxin, which irreversibly damage large subunit rRNA. Although the catalytic activity of ricin toxin A chain (RTA) is well characterized, the precise mechanisms linking ribosome damage to altered cellular morphology remain poorly understood. In this study, we engineered a tetracycline-regulatable expression system to express RTA in Saccharomyces cerevisiae. We characterized the effects of RTA expression on yeast growth, viability, and reproductive potential. We demonstrated that our expression system produced rapid and robust RTA expression and ribosome depurination within less than one …

Population Dynamics And Future Viability Of The Eastern Massasauga Rattlesnake (Sistrurus Catenatus) In Southwest Michigan, Matthew John Klein

Graduate Theses, Dissertations, and Problem Reports (ETD)

Managing at-risk species or populations requires understanding the demographic and genetic drivers of long-term population trends. The eastern massasauga rattlesnake (Sistrurus catenatus) is a federally threatened species facing multiple interacting threats across its range. Habitat loss from wetland drainage and agricultural land conversion has caused range contractions and geographic isolation of populations. Because remaining populations are often small and widely separated, most are now genetically isolated, threatening their long-term viability. Reversing these declines requires understanding population dynamics, quantifying extinction risk, identifying drivers of long-term viability, and assessing the effects of inbreeding and the potential for genetic rescue to …

Survival, Cause-Specific Mortality, Resource Selection, Movement Rates, And Population Dynamics Of White-Tailed Deer (Odocoileus Virginianus) In West Virginia, Usa, Brett P. Skelly

Graduate Theses, Dissertations, and Problem Reports (ETD)

White-tailed deer (Odocoileus virginianus) are one of the most culturally and economically important big game species in the United States. At the turn of the 20th century white-tailed deer were almost extirpated due to market hunting and unsustainable forestry practices that destroyed habitat. The creation of game laws that eliminated market hunting and created seasons and bag limits for deer hunting along with restocking efforts by sportsmen’s groups and state wildlife agencies aided in deer population recovery. These efforts have led to the abundant deer populations that are currently enjoyed by hunters and wildlife viewers. Deer populations require …

An Assessment Of Disease Risks, Population Genetics, And Spatial Ecology Of Appalachian Cottontails (Sylvilagus Obscurus) And Snowshoe Hares (Lepus Americanus) In West Virginia, Usa, Madison Leigh Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Snowshoe hares (Lepus americanus) occupy a large range across the United States and Canada. While snowshoe hare populations are generally considered stable in the interior of their range, populations along range edges are experiencing declines, possibly driven by climate-related factors such as seasonal coat color mismatch. West Virginia represents one of the southernmost extents of the species’ range, yet little is known about the population genetic status or population sizes of snowshoe hares in the state. Appalachian cottontails (Sylvilagus obscurus) are another lagomorph species found in West Virginia. This species experienced a historical decline and currently …

Stakeholder Perceptions Of West Virginia’S Artificial Food Dye Ban In K–12 School Meals: Initial Year Of 2025-2026, Leah Michelle Rogers

Graduate Theses, Dissertations, and Problem Reports (ETD)

OBJECTIVES: Artificial food dyes (AFD+) are an increasing policy concern in the U.S., particularly in children’s foods. West Virginia’s House Bill 2354, the first statewide AFD+ ban implemented in K-12 schools, restricts seven AFD+ in state-funded meals. Little is known about how AFD+ bans are perceived or implemented. This study examined how the ban functioned within school food systems and the barriers and facilitators experienced by school stakeholders within the initial year of implementation during the 2025-2026 school year.

METHODS: A cross-sectional, observational mixed-methods study was conducted among implementers, enforcers, and receivers of the policy. A survey tool …

Surrogate-Based Approaches For Evaluating Post-Harvest Food Safety Interventions In Fresh Produce, Md Shafiul Islam Rion

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pathogenic bacteria are still a threat to human beings worldwide as their presence is often unknown even if they are already present in fresh produces. This in turn resulted in several outbreaks and recalls across the United States. This dissertation investigated the survival of pathogenic bacteria and their potential non-pathogenic surrogates after antimicrobial interventions in different modes of applications on the surface of cantaloupes, butternut squash, and fresh cilantro. In the first study, peroxyacetic acid (PAA) was applied in three separate doses on nalidixic acid resistant Salmonella Typhimurium, Listeria monocytogenes, Enterococcus faecium, and Listeria innocua inoculated cantaloupe surfaces …

An Assessment Of The Reinforcing And Aversive Properties Of Transition-Related Stimuli, Lillian Louise Skiba-Thayer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Transitions from relatively favorable (rich) to relatively unfavorable (lean) activities have been shown to disrupt behavior. Transition-related disruptions in behavior have been observed across laboratories, species, and procedural arrangements. The most commonly reported disruption in basic research is a pause in the reinforced behavior in the signaled transition from a relatively rich to a relatively lean condition of reinforcement (Perone & Courtney, 1992). The present study assessed the function of the stimuli associated with transitions as either reinforcing, punishing, or neutral across two experiments using eight pigeons as subjects. Experiment 1 tested the function of two stimuli, one correlated with …

A Survey Of Disability-Inclusive K-12 Public School Music Programs In The United States Where Best Buddies Friendship Programs Have Been Established, Geena Louise Jeffries Mattox

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines the presence and structural characteristics of disability-inclusive band, orchestra, and choir programs serving students with intellectual and developmental disabilities (IDDs) in K-12 public schools across the United States that implement the Best Buddies School Friendship Program. Intellectual and Developmental Disabilities is defined as group of chronic, lifelong conditions that originate before age 22, affecting physical, learning, language, or behavior areas. Despite federal legislation, peer reviewed scholarship specifically addressing access to performance-based music ensembles for students with IDDs remain comparatively limited. Employing a survey distributed nationwide within a defined population of schools implementing Best Buddies Programs, administered via …

Unsuccessful? Universal Human Rights And The United Nations: Russian Imperialism In Ukraine And The Post-Udhr International System, Sofiia Novytska

Graduate Theses, Dissertations, and Problem Reports (ETD)

Human universality has always stood as a controversial subject and remains politically contentious today. Following the humanitarian crisis of World War II, the international community was determined to protect the integrity and dignity of people worldwide by developing the United Nations’ Universal Declaration of Human Rights. However, universal human rights were not invented by the Declaration and were already conceptually established in global ancient thought, further evolved into Early Modern scholarship, and later paved the way for modern humanitarian frameworks. The UDHR was not universally well-received, and was criticized for its revolutionary legal character, postcolonial contestation, and practical ambiguity. The …

Stakeholder Co-Design Of Sustainable Urban Pest Management Strategies, Elizabeth C. Lowe, Nathan J. Butterworth, Alexander Austin, Cameron Webb, Tanya Latty

Research outputs 2022 to 2026

The high levels of broad-spectrum insecticides used to manage invertebrates in many cities around the world has significant environmental and health impacts. Integrated pest management (IPM) is a framework for sustainable pest control practice, but uptake of IPM remains low in most cities. We used participatory action research with pest management industry stakeholders (practitioners, industry representatives, researchers and government employees) to identify key issues in urban pest management in Australia, set priorities for change and discuss collaborative solutions. Via an online survey and a face-to-face workshop, the participants identified the key themes of education and training, public awareness, environmental impacts …

Inked Mobilities: Tattoos As Embodied Visual Narratives Of Youth Migration Between Italy And Australia, Giulia Marchetti

Research outputs 2022 to 2026

This article examines tattoos as embodied visual narratives within a broader study on youth transnational mobility (2019-2023). Focusing on recent Italian migration to and from Australia, 13 tattoos were documented among the 36 participants, acquired shortly before departure, during the stay in Australia, or upon return to Italy. Methodologically, the article proposes tattoo-based interviews as a participatory visual method that mobilises permanently inscribed images as sites of visual elicitation, enabling forms of reflection and storytelling. Tattoos thus operate as relational spaces of dialogue, co-interpretation, and reflexive meaning-making between researcher and participants. Epistemologically, tattoos are approached as visual narratives through which …

Let’S Make The Change: Addressing The Knowledge Gaps And Building Confidence In Teaching Aboriginal Perspectives For Pre-Service Teachers, Sarah Booth, Libby Jackson-Barrett, Mary Anne Macdonald, Renae Isaacs-Guthridge, Sian Bennett

Research outputs 2022 to 2026

Teaching Aboriginal perspectives and Aboriginal students by non-Indigenous teachers can feel daunting; however, this need not be the case. This study focuses on two frameworks that can be used to motivate pre-service teachers to feel more confident in teaching Aboriginal perspectives even when starting with trepidation in this area. To explain further, this study explored the impact an Aboriginal Contexts in Education unit had on pre-service teachers’ readiness to teach Aboriginal students and Aboriginal curriculum. Through two frameworks pre-service teachers were exposed to a range of Aboriginal perspectives through Aboriginal educators, authors, and resources, and spoke of their appreciation of …

Understanding The Journey To Higher Education: Decision-Making Insights From Aboriginal And Torres Strait Islander Students, Melissa Fong-Emmerson, Claire Lambert, Braden Hill, Maria M. Ryan

Research outputs 2022 to 2026

This study investigates the decision-making process of Indigenous Australian students in pursuing higher education, addressing a gap in understanding their educational choices. It identifies the key factors influencing university enrolment decisions and how these shape students’ educational pathways. Using a narrative-inquiry approach, the study explored the lived experiences of Indigenous students from two Western Australian universities. Through written autobiographies, yarning sessions and one-on-one interviews, participants shared rich, nuanced insights into their journeys to higher education. Findings reveal the pivotal role of ‘sparks’ in normalising higher education as a viable option. Students relied minimally on formal information search, depending heavily on …

Migration Literacy And Commercial Intermediaries: The Debt Financed Labour Migrations Of Chinese Working-Class Tradesmen In Boomtime Australia, Catriona Turnbull

Research outputs 2022 to 2026

This paper explores how constrained informational capital makes migrants dependent upon commercial intermediaries to facilitate their migration. It proposes migration literacy as a critical dimension of mobility capital and as a way to articulate the dependencies engendered through low information capital in relation to the regulatory regimes of the immigration state. These ideas are illustrated with reference to a cohort of working-class labour migrants to Australia whose experiences are shaped by their low migration literacy. Trade skilled migrants from China paid high fees to chains of brokers to secure sponsored employment in Australian workshops. Their low migration literacy, the result …

Periodontitis During Pregnancy: The Effect On The Gut Microbiome And Intestinal Inflammation, Richard Bright, G. Macowan, Keyuan Tian, Tracy Fitzsimmons, Rebecca L. Wilson, Claire T. Roberts, Claus T. Christophersen, Peter M. Bartold, Stephen P. Kidd, Peter S. Zilm

Research outputs 2022 to 2026

Background: Periodontitis has been epidemiologically associated with adverse pregnancy outcomes, but causality remains difficult to establish in humans due to confounding factors. This study uses a controlled murine model to examine the effects of experimentally induced periodontitis on the composition of the gut microbiota and gastrointestinal inflammation during pregnancy. Methods: Periodontitis was induced in pregnant BALB/c mice via oral inoculation with Porphyromonas gingivalis and Fusobacterium nucleatum before conception (n = 20 per group). Pregnancy outcomes, gut histology, systemic inflammatory markers, and microbiome composition, assessed by 16S rRNA sequencing, were evaluated at gestational Day 18. Results: Periodontitis was confirmed by significant …

Insulin Resistance As A Mediator Of Physical Activity's Effects On Beta-Amyloid Accumulation And Tau Phosphorylation: A Scoping Review, Michael G. Slee, Joanne Scotney, Stephanie R. Rainey-Smith, Kirk I. Erickson, Hamid R. Sohrabi, Giuseppe Verdile, Belinda M. Brown

Research outputs 2022 to 2026

Background Type 2 diabetes is associated with increased Alzheimer’s disease risk and brain beta amyloid (Aβ) burden, suggesting an underlying mechanistic relationship between Alzheimer’s disease and type 2 diabetes. Animal studies show exercise reduces levels of brain Aβ and tau, and while human studies are somewhat limited, some studies have reported physical activity is associated with lower brain Aβ and tau levels. Exercise has well established links to reductions in insulin resistance; thus, as physical activity can impact both insulin resistance and Alzheimer’s disease pathology and/or biomarkers, it is reasonable to hypothesise that a mediating relationship may exist. The objective …

A Community Affair: An Exploration Of The Queer Community, Nightlife, Performance Art And Their Adjacent Qualities, Tatiana M. Castro

College of Graduate Studies: Theses & Dissertations

This thesis explores the importance and roll of community in queer artwork, such as drag and burlesque and retroactively also all the work that I create. This paper aims to express this importance by exploring my personal relationship with building community. By interviewing performers selected through a customized-for-savannah voting process, I allowed the community to directly influence my thesis, as well as directly referencing photos of the hands of community members as a continued motif used throughout my work to represent community. This culminated in a two night thesis performance and installation  exhibition that explored performance and how its environment …

Sexual Dimorphism In The Immune Defense Mechanisms Against A Systemic Bacterial Infection In The Cat Flea (Ctenocephalides Felis), Ashley Lynch

College of Graduate Studies: Theses & Dissertations

Fleas defend against infection through the activation of both humoral and cellular innate immune responses. Previous research in female cat fleas demonstrated increased immune activity following a systemic bacterial infection. However, comparable data for males and sex-based comparisons are limited. In this study, we investigated differences in innate cellular immune responses between male and female cat fleas following systemic infection with Gram-negative Escherichia coli. Bacterial load was quantified using colony-forming units 24 h post-infection. Immune function was assessed via phagocytic index and capacity. Survival and total circulating hemocyte counts were evaluated across infected, sterile-injected, and untreated groups. Male fleas …

Differences In Dry Matter Intake In Grazing And Drylot Beef Cattle, Tylor John Yost

Graduate Theses, Dissertations, and Problem Reports (ETD)

Accurate measurement of individual dry matter intake (DMI) in grazing cattle remains a major challenge for evaluating feed efficiency traits in beef production systems. This study evaluated relationships among intake and efficiency traits measured in both pasture and drylot environments and assessed the potential for predicting pasture DMI using water intake and machine learning approaches. A total of 163 beef cattle across four experimental groups were evaluated in one of two grazing seasons (2024 or 2025). Drylot feed and water intake were measured using automated intake monitoring equipment, whereas pasture intake was determined using a long short-term memory …

Cardiovascular–Kidney–Metabolic Syndrome Through The Lens Of Gut‑Derived Uremic Toxins, Chudan Xu, Matthew Snelson, Francine Z. Marques

Research outputs 2022 to 2026

Cardiovascular–Kidney–Metabolic (CKM) syndrome represents a complex, interconnected cluster of cardiovascular disease, chronic kidney disease, and metabolic disorders such as obesity and type 2 diabetes. These conditions share overlapping metabolic, inflammatory, and vascular pathways, with the gut microbiome increasingly recognised as a key contributor and common underlying risk factor. Uremic toxins, traditionally considered waste products of host and microbial metabolism, are now recognised as active mediators of tissue damage across the CKM spectrum, particularly in the context of impaired renal function. Their production and accumulation are amplified by disrupted intestinal barrier integrity, chronic inflammation, and reduced renal clearance, collectively driving systemic …

Forecasting The Emotional Cost Of Online Dehumanization, Reyhaneh Bagherian Shamir

All Master's Theses

The use of social media platforms to share, view, and communicate information is now ubiquitous (Crockett, 2017). Given the prevalence of problematic content found on social media (e.g., prejudice, misinformation), this research examines how accurately people predict their responses to such content. Kawakami et al. (2009) found that individuals overestimate their emotional distress and behavior in response to discriminatory actions (i.e., racism) in-person predicting they would be more upset, and more likely to reject a racist confederate than individuals who experienced the incident actually were. In the current research, we built upon the work of Kawakami et al. (2009) to …

Modernism’S Wild Child: The Interplay Of Modernist And Children’S Literature, Rachel Riffel

All Master's Theses

Within literary criticism, American and British modernism are often characterized by the disillusioned writers and creators who established an elitist literary community during the early 20^th century. This period is also distinguished by the Harlem Renaissance highlighting Black art, culture, and writing. Gertrude Stein, T. S. Eliot, Langston Hughes and Arna Bontemps are well-known for contributing core texts to modernism and the Harlem Renaissance that expressed existential themes and developed experimental techniques. What many readers don’t know, however, is that these authors and many other contemporaries wrote stories and poetry for children. In this thesis, I explore the children’s …

Examining The Effects Of Virtual Nature Exposure And Video Immersion On State Hope With Nature Relatedness As A Covariate, Zachary Arneson

All Master's Theses

Previous work in the field of environmental psychology has shown that virtual nature exposure can produce many psychological benefits for the viewer. Within this literature, however, there has been no examination of the effects of virtual nature exposure on levels of state hope, a goal-directed positive psychological construct made up of two types of thought: agency and pathways. Additionally, there has been no direct examination of the effect of immersion level of the virtual nature exposure on psychological measures. To fill this gap in the literature, the current study examined the effects of virtual nature exposure and video immersion on …

Pre-Eruptive Timescales Via Olivine Diffusion Chronometry For Lava And Tephra Of The Schreibers Meadow Cinder Cone Eruption, Mount Baker (Kulshan), Wa, Sabrina Ansari

All Master's Theses

Mount Baker is a very-high threat Cascade volcano and is located near densely populated areas in northern Washington, putting many at risk if an eruption were to occur. Schreibers Meadow cinder cone is located on the southern flank of Mount Baker and produced the Sulphur Creek (SC) tephra and lava 9.8 ka. Previous work on the SC eruption used petrology and geochemistry to reconstruct the pre-eruptive magmatic architecture beneath Mount Baker Volcanic Field, including olivine-hosted melt inclusions to determine crystallization pressures, as well as plagioclase- and clinopyroxene-derived diffusion timescales to determine magma storage timescales of several months and recharge-to-eruption timescales …

Gis Is A Tool, Not The Story: The Journey Towards An Ethnographically Informed Gis Analysis Of Frenchman Coulee, Eastern Washington, Joseph T. Gambino

All Master's Theses

Geographic Information Systems (GIS) have seen increased use in ethnographic and archaeological studies over the last several decades. Though revolutionary in their ability to predict and document sites of archaeological or cultural significance, they remain limited in their capacity to communicate the full extent of potential values certain geographies offer to both cultural specialists and their communities. These limitations are amplified when attempting to represent how the landscape is experienced within GIS’s statistical framework. In this manuscript I present different approaches to GIS modeling that attempt to identify some of the advantages Frenchman Coulee could have offered to the Indigenous …

Can Environmentally Friendly Hotels Lead Customers To Green Behaviors? Evidence From The Stimulus–Organism–Response Model And Social Cognitive Theory, Saeid Nosrati, Mohtaram Rabbani, Razieh Sharifipur Shirazi, Ilkay Yorganci, Niusha Talebzadeh, Negin Bassirat

Research outputs 2022 to 2026

Purpose Using the stimulus–organism–response model and social cognitive theory, this study aims to examine how environmentally friendly hotels influence customers’ green patronage intention and green word-of-mouth. The study explored the mediating effects of hedonic value and utilitarian value and the moderating role of environmental concern. Design/methodology/approach Data were collected from 200 customers of environmentally friendly hotels in Iran and analyzed using structural equation modeling (SEM) with Mplus (version 8.3) software. Findings The results indicate that environmentally friendly hotels positively influence hedonic and utilitarian values, as well as green patronage intention and green word-of-mouth. Additionally, hedonic and utilitarian values partially mediate …

Spatio-Temporal Responses Of A Migratory Shorebird Community Reflect Complex Trade-Offs Among Overabundance Of Predators And Disturbance, K. M. Aarif, Aymen Nefla, Zuzana Musilova, Petr Musil, Sora M. Estrella

Research outputs 2022 to 2026

Animal selection of foraging grounds is a trade-off among available feeding resources, intra and interspecific competition, disturbance and predation risks. The present study was carried out at Kadalundi-Vallikkunnu Community Reserve on the West coast of Kerala (India) in the Central Asian Flyway. The Reserve hosts different coastal habitats, including mudflats, mangroves and sand beaches, and although 31 species of shorebirds are commonly present, it is heavily impacted by human activities. The findings highlight the necessity of robust management of anthropogenic waste and reveal the detrimental impacts of human activities on species communities of conservation concern. We investigated how the densities …

Noongar Culture, Arts Learning, And Wellbeing Literacy In Early Childhood: A Case Study Of The Song Room’S Deadly Arts Early Years Program In Western Australia Government Primary Schools, Jason Goopy, Narelle Lemon, Megan Mcpherson, Libby Jackson-Barrett, Jacqueline West

Research outputs 2022 to 2026

This report presents findings from a case study examining how The Song Room's Deadly Arts Early Years program supports wellbeing literacy in early childhood education through engagement with Noongar culture, arts, and storytelling at Western Australia government primary schools. Conducted on Whadjuk Noongar Boodjar at Huntingdale Primary School and Orelia Primary School, the research was co-designed with Indigenous and non-Indigenous teaching artists, school communities, and The Song Room.

The study adopted wellbeing literacy — the capability to understand and intentionally use language about and for wellbeing across contexts — as its theoretical framework. Creative arts-based methods, including draw and tell …

Association Between Protein Intake From Animal And Plant Sources And The Presence Of Frailty In Community-Dwelling Australian Women, Trent Bozanich, Jack Dalla Via, Simone Radavelli-Bagatini, Cassandra Smith, Elsa Dent, Anthony Villani, Robin M. Daly, Michael Tieland, Jonathan M. Hodgson, Kun Zhu, Richard L. Prince, Marc Sim

Research outputs 2022 to 2026

We investigated the cross-sectional association between total, animal and plant protein intake with the presence of a multidimensional measure of frailty in older women. Participants included 1380 community-dwelling Australian women, mean (SD) age 75.1 (2.7) years. A validated food frequency questionnaire was used to determine total, plant, and animal protein intake. Frailty was assessed using a frailty index across numerous health domains (scored 0–1, ≥0.25 indicating frailty). Cross-sectional associations were analysed using multivariable-adjusted logistic regression models. In total, 275 (19.9%) women were identified as frail. A non-linear inverse association was observed between higher intakes of total protein and the odds …

Diet, Gut Microbiota, And Intestinal Permeability: Emerging Mechanisms In Hypertension Pathogenesis, Esha Vashisht, Helen Nguyen, Francine Z. Marques, Matthew Snelson

Research outputs 2022 to 2026

Hypertension is a leading contributor to global morbidity and mortality, arising from the interplay of genetic and environmental risk factors together with the dysregulation of multiple physiological systems involved in blood pressure control. Recent advances have established the gut as a central regulator, implicating the intestinal microbiota and barrier integrity in the modulation of blood pressure. Alterations in the gut microbial consortium, along with changes in levels of metabolites produced by the microbiota, have been associated with blood pressure regulation in animal models and human studies. Key microbial metabolites, including short-chain fatty acids, trimethylamine-N-oxide, and bile acids influence endothelial function, …