Digital Commons Network^™

Citric Acid/Β-Alanine Carbon Dots As A Novel Tool For Delivery Of Plasmid Dna Into E. Coli Cells, Anju Pandey, Asmita Devkota, Anil Sigdel, Zeinab Yadegari, C. Korsi Dumenyo, Ali Taheri

Agricultural and Environmental Sciences Faculty Research

Successful delivery of plasmid DNA into the microbial cells is fundamental in recombinant DNA technology. Natural bacterial transformation is limited to only certain species due in part to the repulsive forces between negatively charged DNA and bacterial membranes. Most common method of DNA delivery into bacteria is artificial transformation through heat shock and electroporation. These methods require sophisticated instruments and tedious steps in preparation of competent cells. Transformation by conjugation is also not applicable to all plasmids. Nanoparticles have been used successfully in therapeutics for drug delivery into animal cells. They are starting to gain popularity in plant sciences as …

Single-Cell Rna Sequencing (Scrna-Seq) Exploratory Analyses To Investigate Aging-Dependent Changes And Regional Heterogeneity In Glial Cells, Valerie N. Doze

CSB and SJU Distinguished Thesis

My thesis project research consisted of single-cell RNA-sequencing and bioinformatics using R programming and Neuroscience Multi-omic Data Archive (NeMO) Analytics to examine the impact of aging, brain region, and other variables on the genotypes and phenotypes of oligodendrocytes (OLGs) and oligodendrocyte precursor cells (OPCs). The overarching goal was to increase understanding of glial cell fate and function. After determining bioinformatical findings, follow-up wet-bench experiments were performed to validate results. RNA in situ hybridization and immunohistochemistry were performed to confirm differential gene expression across cell types and brain regions. These experiments also revealed any changes in oligodendrocyte (OLG) development and maturation …

Richard Abbott V. Luke Mette

2021 Decisions

USDC for the District of Delaware

Sighted: Skinwalkers, Lexie Russon

Final Projects

Stories about people who have encountered Navajo Skinwalkers.

Comparison Of Methods To Estimate Ruminal Degradation And Intestinal Digestibility Of Protein In Hydrolyzed Feather Meal With Or Without Blood, Kassidy Buse, D. L. Morris, H. L. Diaz, O. R. Drehmel, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Hydrolyzed feather meal (HFM) is a feedstuff high in rumen undegraded protein (RUP) that can be used as an effective source of metabolizable protein for dairy cattle. Because the production process may vary, the rumen degradability and intestinal digestibility of HFM may also vary. Additionally, some processes may incorporate additional blood into the final product to result in feather meal with poultry blood. To determine the rumen degradability and intestinal digestibility of these products, several laboratory assays can be used; the common assays are the mobile bag (MOB), modified three-step (MTS), and Ross (ROS) assays. Although all 3 assays determine …

Online Meme Community Of Fire Emblem: Three Houses, Clelland Ukiah Reynolds

Final Projects

About an online community known as Community of Fire Emblem: Three Houses. This paper examines how folklore is used to create community.

The Validating Formula: Convergence Of Folklore With Internet Culture, Regan Poulsen

Final Projects

This paper analyzes how folklore is increasingly transmitted online rather than face to face

Sophisticated Scares: An Analysis Of Scary Stories, Abigail Tree

Final Projects

This paper argues that scary stories follow a sophisticated oral narrative pattern, which reveal the concerns of contemporary society

Hungry Ghosts, Ming Li Yew

Final Projects

About supernatural events that have occurred during Hungry Ghost Festivals

Bet You Won't Guess My Middle Name: Middle Name Customs In Lds Culture, Kayla Rane

Final Projects

About the Latter-day Saint tradition of withholding a daughter's middle name. Includes interviews about women who legally adopted their maiden name as a middle name upon marriage.

Dachy's Deaf, Hadassa Jean-Louis

Diverse Families Bookshelf Lesson Plans and Activities

The student will be able to retell a text using story elements (characters, setting, beginning, middle and end) to enhance comprehension. ● The student will be able to identify ways people can make positive contributions in their community through kindness.

Approved Programs 12-14-21, Angela Brock

Programs Committee (PC)

No abstract provided.

Programs Agenda 12-14-21, Angela Brock

Programs Committee (PC)

No abstract provided.

Public Opinion And Knowledge Of Marine Biodiversity In A Coastal Community - Horry County, South Carolina, Arianna R. Trapp

Honors Theses

There is very little research that explores the public attitude toward marine biodiversity loss as an environmental issue. This study aims to provide insight into the disparities in marine conservation education in order to better provide meaningful education opportunities for coastal communities. The data for this research was collected using a series of semi-structured interviews that were conducted as focus groups of participants from Horry County, South Carolina. Residents from North Myrtle Beach, Myrtle Beach, Loris, and Bucksport were recruited to three focus groups based on their geographical residency. Horry County retirees and students attending Coastal Carolina University were also …

December 2021 Meeting Minutes, Angela Brock

UCC Agendas/Minutes

No abstract provided.

Consultation/Liaison Psychiatry During Covid-19, Robert Frierson, Steven B Lippmann

The University of Louisville Journal of Respiratory Infections

Consultation/liaison psychiatrists care for people with co-existing medical and psychologic difficulties. The coronavirus pandemic is stressful for patients and their psychiatric caregivers. Patients have lost support systems and harbor fears about exposing family to the virus. COVID-19 sometimes exacerbates previous psychiatric conditions, while diminishing intimacy with physicians. Everyone is oversaturated with COVID-19-related news focusing on rising concerns about the illness and about jobs, school, and housing insecurities.

The psychiatrist maintains a hospital presence despite fear of contracting the disease. Challenges include addressing staff morale, evidencing empathy despite telecommunication devices, and treating anxiety, depression, sleep phobias, post intubation flashbacks, COVID-19-induced cognitive …

A Link Between Methylglyoxal And Heart Failure During Hiv-1 Infection, Prasanta K. Dasg, Fadhel A. Alomar, Jesse L. Cox, Joellyn Mcmillan, Bryan T. Hackfort, Edward Makarov, Brenda Morsey, Howard S. Fox, Howard E. Gendelman, Santhi Gorantla, Keshore R. Bidasee

Department of Biochemistry: Faculty Publications

Early-onset heart failure (HF) continues to be a major cause of morbidity and mortality in people living with human immunodeficiency virus type one (HIV-1) infection (PLWH), yet the molecular causes for this remain poorly understood. Herein NOD.Cg- Prkdc^scidIl2rg^tm1Wjl/SzJ humanized mice (Hu-mice), plasma from PLWH, and autopsied cardiac tissues from deceased HIV seropositive individuals were used to assess if there is a link between the glycolysis byproduct methylglyoxal (MG) and HF in the setting of HIV-1 infection. At five weeks post HIV infection, Hu-mice developed grade III-IV diastolic dysfunction (DD) with an associated two-fold increase in plasma …

Uji Aktivitas Protease Empat Isolat Trichoderma Spp. Yang Berasal Dari Tanah Perakaran, Fitria Dewi Sulistiyono, Loekas Soesanto, Nuniek Ina Ratnaningtyas

Chimica et Natura Acta

Perkembangan industri protease telah pesat dan menempati posisi penting dalam bidang industri. Protease dapat diperoleh dari semua mahluk hidup, namun saat ini pemakai enzim banyak yang beralih pada enzim mikrobioal. Keunggulan enzim microbial adalah produksi tinggi, kemudahan dan efisiensi dalam pengaturan pertumbuhan, limbah pertanian dapat dimanfaatkan sebagai sumber substrat. Salah satu mikroba adalah Trichoderma spp. Trichoderma sp dapat berasal dari tanah perakaran tanaman (rhizosfer). Jenis tanah perakaran diantarnya adalah jahe, nenas, pisang dan bawang merah. Keempat tanaman ini merupakan tanaman yang banyak dibudidayakan oleh masyarakat. Tujuan penelitian ini adalah menentukan jumLah protease yang terdapat pada Trichoderma spp. Metode penelitian dilakukan …

2021-12-14 Message From The President, Morehead State University. Office Of The President.

Staff Congress Records

A message sent by the President of Morehead State University to the campus community regarding upcoming dates of importance, announcements and the campus closing from December 14, 2021.

The G7 Summit 2021: Time For Our World Leaders To Step Up To The Challenge Of Anti-Microbial Resistance, Lt Joshi

Peninsula Dental School

In June 2021, I wrote a strongly worded opinion piece published in the New Statesman about the G7 Summit and antimicrobial resistance (AMR) [1]. The piece highlighted that AMR should be addressed in the G7 Summit agenda and that our world leaders must recognize that research and investment into feasible solutions are urgently needed. This personal view adds to that original article. The G7 Summit took place in Carbis Bay, Cornwall, UK on the 11–13 June 2021 and the UK took Presidency of the G7 group of nations [2]. The G7 comprises seven member countries, namely the UK, the USA, …

Inhibition Of Nadph Oxidase Blocks Netosis And Reduces Thrombosis In Heparin-Induced Thrombocytopenia, Halina H L Leung, Jose Perdomo, Zohra Ahmadi, Feng Yan, Steven E. Mckenzie, Beng H Chong

Department of Medicine Faculty Papers

Heparin-induced thrombocytopenia (HIT) is associated with severe and potentially lethal thrombotic complications. NETosis was recently shown to be an important driver of thrombosis in HIT. We investigated the role of reactive oxygen species (ROS) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) and their contributions to thrombus development in HIT. We showed that neutrophil activation by HIT immune complexes induced ROS-dependent NETosis. Analysis of thrombi formed in a microfluidics system showed ROS production in both platelets and neutrophils, and abundant neutrophil extracellular traps (NETs) and ROS distributed throughout the clot. Neutrophil-targeted ROS inhibition was sufficient to block HIT-induced NETosis …

Two Moons, Chuhan Peng

Theses

People may think that they can better understand the world through convenient technology in this Internet age. The fact is that even though we can search almost everything on Google, we still live and are confined to a small world. What's worse, big smart data will provide you with "privately customized" information services based on your specific situation. People gradually become accustomed to a voice and are more inclined to think that the world is what they see. This has also led to the fact that people of different social classes and cultural environments may be very different, and they …

Suno ("Listen"), Kajal Kohli

Theses

Suno (“Listen” in Hindi) is a 3D Animated Short that revolves around our main character Sid’s day in his life where he decides to find someone who would listen to him about what he’s going through without passing judgement of their own. Sid is an 18-year-old boy living in a typical Indian household with his parents and grandmother. Sid has always been a brilliant student academically and has had accolades in sports rendering him in what one may call an astounding over-all achiever. Even so, he has started to feel burned out and depressed. He has gone into his own …

Lipid A-Ara4n As An Alternate Pathway For (Colistin) Resistance In Klebsiella Pneumonia Isolates In Pakistan, Kiran I. Masood, Seema Umar, Zahra Hasan, Joveria Farooqi, Safina Abdul Razzak, Nazish Jabeen, Jason Rao Jason Rao, Sadia Shakoor, Rumina Hasan

Department of Pathology and Laboratory Medicine

Objectives: This study aimed to explore mechanism of colistin resistance amongst Klebsiella pneumoniae isolates through plasmid mediated mcr-1 gene in Pakistan. Carbapenem and Colistin resistant K. pneumoniae isolates (n = 34) stored at - 80 °C as part of the Aga Khan University Clinical Laboratory strain bank were randomly selected and subjected to mcr-1 gene PCR. To investigate mechanisms of resistance, other than plasmid mediated mcr-1 gene, whole genome sequencing was performed on 8 clinical isolates, including 6 with colistin resistance (MIC > 4 μg/ml) and 2 with intermediate resistance to colistin (MIC > 2 μg/ml).
Results: RT-PCR conducted revealed absence of …

A Comparison Of The Risk Of Fracture In Rheumatoid Arthritis Patients With And Without Receiving Chinese Herbal Medicine, Hou-Hsun Liao, Hanoch Livneh, Yu-Jung Chung, Ching-Hsing Lin, Ning-Sheng Lai, Hung-Rong Yen, Tzung-Yi Tsai

Counselor Education Faculty Publications and Presentations

Objective: Patients with rheumatoid arthritis (RA) often suffer from bone complications due to persistent joint inflammation, especially incident fracture. Nowadays, Chinese herbal medicines (CHMs) have provided safe and effective therapy for treating skeletal conditions, but it is unclear whether CHMs can prevent fracture onset among RA individuals. This study aimed to determine the association between the use of CHMs and the risk of fracture among them.

Methods: This retrospective, population-based study retrieved administrative health data from the Taiwan National Health Insurance (NHI) database to identify patients with newly diagnosed RA between 2000 and 2009. Of the 6178 incident …

Defining The Transcriptional Control Of Pediatric Aml Highlights Rara As A Superenhancer-Regulated Druggable Dependency, Monika W Perez, Oscar Sias-Garcia, Alfred Daramola, Helen Wei, Maci Terrell, Raushan Rashid, Woojun D Park, Kevin Duong, Terzah M Horton, Feng Li, Nikitha Cherayil, Jost Vrabic Koren, Vincent U Gant, Jacob J Junco, Choladda V Curry, Alexandra M Stevens, Charles Y Lin, Joanna S Yi

Faculty, Staff and Students Publications

Somatic mutations are rare in pediatric acute myeloid leukemia (pAML), indicating that alternate strategies are needed to identify targetable dependencies. We performed the first enhancer mapping of pAML in 22 patient samples. Generally, pAML samples were distinct from adult AML samples, and MLL (KMT2A)-rearranged samples were also distinct from non-KMT2A-rearranged samples. Focusing specifically on superenhancers (SEs), we identified SEs associated with many known leukemia regulators. The retinoic acid receptor alpha (RARA) gene was differentially regulated in our cohort, and a RARA-associated SE was detected in 64% of the study cohort across all cytogenetic and molecular subtypes tested. RARA SE+ pAML …

Mechanization: Planning And Selection Of Equipment, C. A. Rotz

IGC Proceedings (1977-2023)

The planning and selection of equipment for harvest and handling of forage crops can greatly impact the performance and profitability of a farm. The type and size of equipment used affects the harvested yield and nutritive value of the forage crop as well as production costs. Through interactions with other parts of the farm, these effects can impact market value of the forage, animal intake and performance, delays in other farm operations, other production costs, and ultimately farm profit. Standard procedures and relationships have been developed for determining the most appropriate equipment for a given production system. Because of the …

Conserved Forage (Silage And Hay): Progress And Priorities, R. E. Muck, K. J. Shinners

IGC Proceedings (1977-2023)

Forage conservation permits a better supply of quality feed when forage production is low. While haymaking and ensiling have been practiced for generations, research is still needed to 1) understand the processes affecting quality during harvesting and storage and 2) develop practical means to minimize losses and enhance quality.

Several trends in harvesting forages for silage are notable. Kernel processing of maize, once confined to Europe, has become popular in North America. Self-propelled forage harvesters have a larger share of the market due to more contract harvesting and larger farms. Larger harvesters, rakes and mergers help improve productivity and forage …

Wagon Tracks Volume 36, Issue 1 (November 2021)

Wagon Tracks

Contents

2 On the Cover: East Meets West, by Cally Krallman

4 Insights from your President

5 Joanne’s Jottings

2, 6-9, 13, 19 Trail News

10-12 Remembering Faye Gaines

14 Faces at the Symposium By Dotti Russell

15-20 A Million Ways to Die in the West: Three Pandemics by John F. Steinle

21-28 Santa Fe Trail Association Hall of Fame Inductees

29-35 Chapter Reports

33 Membership Form

36 Calendar

Two Ghost Poems, Casey Fuller

Floodwall Magazine

No abstract provided.