Digital Commons Network^™

The Alice Atom Compendium, Nick Mendillo

Open Access Master's Theses

Using an original work of fiction, a novel I had written titled The Alice Atom, I have created multiple levels of media that encompass several aspects of said novel’s central theme – the desire to find meaning in not only products we create but the act of ‘following one’s dream.’ To achieve a personal goal is to follow, chase, or accomplish their ‘dreams,’ but I intended to explore the question of what that pursuit can tangibly look like. The action of the novel involves the protagonist literally searching for a woman he has imbued with deep philosophical meaning. I, …

Do Environmentally Relevant Concentrations Of Microplastics Pose A Threat To The Eastern Oyster, Crassostrea Virginica?, Hannah Haskell

Open Access Master's Theses

It is well known that microplastics are ubiquitous within the marine environment and that organisms are interacting with them. However, information on the potential impacts this pollutant may have on an organism are less well understood, especially in response to environmentally relevant microplastic concentrations The aim of this study was to determine if environmentally relevant microplastic exposure has any physiological impact on the eastern oyster, Crassostrea virginica. We exposed C. virginica to daily doses of polyester microfibers based on concentrations reported from the field (0, 2, & 95 fibers L-1) as well as a higher concentration similar to those …

Translation And Validation Of Myeloid Derived Suppressor Cell Proteomic Targets In Liver And Lung Tumors, Justin Trickett

Open Access Master's Theses

Advancements in technology have made possible the development of highly personalized and efficacious medicines. Various forms of immunotherapy have demonstrated clinical utility in the treatment of blood-based cancers (e.g., leukemia, lymphoma, etc.). Cells in the tumor microenvironment (TME) of solid tumors can suppress the immune response of T cells, therefore hindering the efficacy of T-cell immunotherapies. Consequently, a growing number of studies are investigating methods of modulating the TME for improving immunotherapeutic outcomes in this context. Myeloid-derived suppressor cells (MDSCs) are one such cell responsible for diminishing the activity of immune cells by secreting immunosuppressive cytokines. Recent studies show that …

Bioconcentration Of Pfas From Afff Environmental Exposure: Evidence Of Precursor Bioaccumulation In Fathead Minnows, Nicholas Hill

Open Access Master's Theses

Exposure to per- and polyfluoroalkyl substances (PFAS) has been associated with toxicities in wildlife and negative health effects in humans. Decades of fire training activity at Joint Base, Cape Cod, Massachusetts incorporated the use of aqueous film forming foam (AFFF) which resulted in long-term PFAS contamination of sediments, groundwater, and hydrologically connected surface waters. To explore the bioaccumulative potential of PFAS residing in complex environmental mixtures, a mesocosm was established to evaluate bioconcentration of PFAS from AFFF-contaminated groundwater by flow-through design. Fathead minnows (n = 24) were exposed to PFAS in groundwater over a 21-day period and tissue specific PFAS …

Parallelized Implementation Of A Genetic Algorithm On A Field Programmable Gate Array (Fpga) To Provide Heuristic Solutions For The Capacitated Vehicle Routing Problem (Cvrp), Maximilian Jakob Heer

Open Access Master's Theses

This thesis reports on the implementation of a Genetic Algorithm for finding heuristic solutions to the Capacitated Vehicle Routing Problem (CVRP) on High-Performance Field Programmable Gate Arrays (FPGAs). The heuristic approach to the class of NP-hard problems related to the Travelling-Salesman-Problem (TSP) has a long tradition in computer science, with this thesis being directly based on an implementation of a specialized Genetic Algorithm for a Graphic Processing Unit (GPU). In my work, I describe how the algorithmic structure can be directly transformed into an adapted computing architecture with special focus on the highest possible degree of parallelism. For that, the …

Expert Elicitation Regarding Effectiveness Of Recent North Atlantic Right Whale Protection Agreement, Jessica Marie Hiltz

Open Access Master's Theses

With the rise of offshore wind energy in the United States, there has been increasing concern about how offshore wind turbine installation and operation is affecting benthic and pelagic marine life (Bailey et al. 2010). Increased, localized ocean noise resulting from project-related activities, such as vessel traffic, surveying, and turbine installation, has the potential to disturb or injure nearby marine life. Increased vessel traffic heightens the probability of a vessel strike occurring, and noise produced during the turbine installation phase, in which pile-driving is used to hammer and secure turbines to the seafloor, is significantly loud and widespread. This noise …

Modeling Rhode Island Food Production Potential Under Different Production Scenarios, Nina Oberg

Open Access Master's Theses

Food sovereignty emphasizes the rights of people to have control over their food system. Relocalizing food production and limiting dependence on global industrial supply chains is necessary to increase food sovereignty in regions dominated by conventional food systems. In the US, the New England region aims to relocalize production by producing 50 percent of food consumed in the region by the year 2060. Rhode Island can contribute to this goal by expanding production and strengthening local food systems, thus serving as a model for New England and other urbanizing regions globally. For this study, a site suitability analysis was performed …

Home Grown: An Experimental Analysis Of Home-Methods Of Mold Removal On Textile Substrates, Paige Bailey

Open Access Master's Theses

When seeking information on how to clean away mold from clothing and textiles, searching the internet can lead to an overwhelming amount of confusing and vague information. A lack of reputable references and consistent measurements across methodologies makes attempting to clean textiles, valuable or not, potentially damaging to the textile itself. As such, the objective of this experiment was to select a handful of these cleaning methods and test them with the expectation that the results will contribute to reliable information on what methods could possibly work for certain textiles. It is the hope that this information will be made …

Treprostinil Improves Hepatic Cytochrome P450 Metabolism During Renal Ischemia Reperfusion Injury, Daniel Kelly

Open Access Master's Theses

Background: Renal ischemia-reperfusion injury (IRI) causes acute kidney injury, as well as liver injury resulting in altered hepatic metabolism. Decreased hepatic cytochrome P450 levels can impact drug clearance and increase the risk for adverse drug reactions. Currently, there is no treatment available to improve liver cyp450 metabolism during renal IRI. Treprostinil (Remodulin®) is a FDA approved prostacyclin analog that our lab has shown to have hepatoprotective effects during renal IRI. This study investigates the efficacy of treprostinil on improving hepatic cyp450 metabolism during renal IRI in vivo.

Methods: Male Sprague Dawley rats were subjected to 45 minutes of …

Using Centered Kernel Alignment For Understanding And Pruning Neural Networks, Alex Lachance

Open Access Master's Theses

Understanding neural networks has been challenging since their inception. Neural networks are non-linear and learn representations without the user defining them, making the internal workings obscure. Methods for understanding networks have been researched for many years. This study uses centered kernel alignment (CKA) to measure the similarity between the representations of data between layers in a network. The method is invariant to invertible orthogonal transformation, invariant to isotropic scaling, and exhibits behavior demonstrating its superiority over other methods.

The similarity results enable understanding of how architecture choices impact learning and network representations. Depth and width changes of MLPMixer, a neural …

The Structure, Constraints, And Suggestions Of The Indian Medical Textile Product Industry With A Focus On Fabric Manufacturers, Poonam Parab

Open Access Master's Theses

Medical textiles are specialty fabrics and products doctors, nurses, and other healthcare workers use. These include bandages, surgical gowns, hospital sheets, artificial hearts, and hernia meshes. India has been a global textile manufacturing hub and has recently shifted its focus to medical textile/ textile products. However, there is no clear understanding of the basic structure of the Indian medical textile product industry related types of companies involved and their interactions with each other. The information about the industry needs to include categorization and depth, and there is no value chain-related information. A well-integrated value chain improves efficiency and effectiveness, which …

Peruvian Mammals Collected By The Andrews University. Department Of Biology : Expeditions & Patterns Of Diversity, Kieran M. Taylor

Master's Theses

The Department of Biology at Andrews University conducted three expeditions to Peru in 1964, 1965, and 1968. During the expeditions, thousands of specimens were collected of several taxa, including mammals. As far as we know, no complete summary of the expeditions and the mammals collected has been created. Knowledge of the expeditions and evaluation of the mammals collected compared to current literature could have conservation implications. My purpose was to recreate the story of the expeditions and use this contextual information to catalog and identify the number of mammal species collected and evaluate the mammal diversity collected according to current …

Unleashing Pets From Dead-Hand Control, Kaity Emerson, Kevin Bennardo

Faculty Publications

Many pet owners feel strongly about their animals. Some feel so strongly that they desire their pets to accompany them to the grave. This Article addresses the validity of pet euthanasia provisions in decedents’ wills.

Pet owners generally have the legal power to humanely euthanize their pets. In addition, the primary focus of the law of wills is to effectuate the wishes of the decedent. These two facts seem to counsel in favor of carrying out a testamentary instruction to humanely euthanize a companion animal. Yet courts generally decline to enforce pet euthanasia provisions whenever an objection is raised by …

The Last Breakfast With Aunt Jemima And Its Impact On Trademark Theory, Deborah R. Gerhardt

Faculty Publications

The generally-accepted law and economics theory of trademarks fails to explain why a brand owner would ever walk away from a trademark that generates financially lucrative returns. In 2020, that is exactly what happened again and again as brand owners pledged to abandon racially explicit marks in the weeks following George Floyd’s murder. As citizens became more attuned to the experiences of those depicted in racial marks, the owners of Aunt Jemima, Uncle Ben’s, the Cleveland Indians, the Redskins, the Dixie Chicks, Lady Antebellum and others announced these brands’ days were numbered. By evoking racist stereotypes, they became a moral …

Superior Status: Relational Obstacles In Law To Racial Justice And Lgbtq Equality, Osamudia James

Faculty Publications

Animus and discrimination are the two legal lenses through which in-equality is typically assessed and understood. Insufficient attention, however, is paid to the role of status in animating inequality, even in landmark cases thought to be equality-promoting. More than an animating force between intractable po-litical conflicts, status also informs the development of equality law in the United States. When courts, advocates, and policymakers affirm, ignore, miss, or con-cede to status hierarchies instead of dismantling them, those groups that perceive a decrease in their status relative to others will only use “equality-promoting” doctrine to rebalance status hierarchy in their favor. Public …

Automated Legal Guidance At Federal Agencies, Joshua D. Blank, Leigh Z. Osofsky

Faculty Publications

When individuals have questions about Federal benefits, services, and legal rules, they are increasingly seeking help from government chatbots, virtual assistants, and other automated tools. Current forms of automated legal guidance platforms include the U.S. Citizenship and Immigration Services’s “Emma,” the U.S. Department of Education’s “Aidan,” and the Internal Revenue Service’s “Interactive Tax Assistant.” Most scholars who have studied artificial intelligence and Federal government agencies have not focused on the government’s use of technology to offer guidance to the public. The absence of scholarly attention to automation as a means of communicating government guidance is an important gap in the …

First Comes Love. Then Comes Marriage. Then Comes A Baby In A Baby Carriage: An Application Of Protective Surrogacy Laws To The Tarheel State, Justin Lo

Seattle University Law Review

Assisted Reproductive Technology (ART) and determining parentage have a common feature: each is governed by state law or the lack of such laws. This lack of statutory regulations presents significant legal challenges to gay men who wish to start a family. Because same-sex male couples seeking to become fathers through ART and surrogacy are the most likely demographic to be impacted when determining parentage, laws that influence the direction of surrogacy will undeniably facilitate whether both males will be deemed a father. To provide same-sex male couples with a pathway to parenthood, North Carolina should (1) develop robust, protective surrogacy …

High Temporal Resolution Measurements Of Movement Reveal Novel Early-Life Physiological Decline In C. Elegans, Drew Benjamin Sinha, Zachary Scott Pincus

Open Access Publications

Age-related physiological changes are most notable and best-studied late in life, while the nature of aging in early- or middle-aged individuals has not been explored as thoroughly. In C. elegans, many studies of movement vs. age generally focus on three distinct phases: sustained, youthful movement; onset of rapidly progressing impairment; and gross immobility. We investigated whether this first period of early-life adult movement is a sustained "healthy" level of high function followed by a discrete "movement catastrophe"-or whether there are early-life changes in movement that precede future physiological declines. To determine how movement varies during early adult life, we followed …

Advances And Gaps In Sars-Cov-2 Infection Models, César Muñoz-Fontela, Michael S. Diamond, Et Al

Open Access Publications

The global response to Coronavirus Disease 2019 (COVID-19) is now facing new challenges such as vaccine inequity and the emergence of SARS-CoV-2 variants of concern (VOCs). Preclinical models of disease, in particular animal models, are essential to investigate VOC pathogenesis, vaccine correlates of protection and postexposure therapies. Here, we provide an update from the World Health Organization (WHO) COVID-19 modeling expert group (WHO-COM) assembled by WHO, regarding advances in preclinical models. In particular, we discuss how animal model research is playing a key role to evaluate VOC virulence, transmission and immune escape, and how animal models are being refined to …

Disruption Of Thymic Central Tolerance By Infection With Murine Roseolovirus Induces Autoimmune Gastritis, Tarin M. Bigley, Liping Yang, Liang-I Kang, Jose B. Saenz, Francisco Victorino, Wayne M. Yokoyama

Open Access Publications

Infections with herpesviruses, including human roseoloviruses, have been proposed to cause autoimmune disease, but defining a causal relationship and mechanism has been difficult due to the ubiquitous nature of infection and development of autoimmunity long after acute infection. Murine roseolovirus (MRV) is highly related to human roseoloviruses. Herein we show that neonatal MRV infection induced autoimmune gastritis (AIG) in adult mice in the absence of ongoing infection. MRV-induced AIG was dependent on replication during the neonatal period and was CD4+ T cell and IL-17 dependent. Moreover, neonatal MRV infection was associated with development of a wide array of autoantibodies in …

Sonobiopsy For Minimally Invasive, Spatiotemporally-Controlled, And Sensitive Detection Of Glioblastoma-Derived Circulating Tumor Dna, Christopher Pacia, Jinyun Yuan, Yimei Yue, Lu Xu, Arash Nazeri, Rupen Desai, H. Michael Gach, Xiaowei Wang, Michael Talcott, Aadel Chaudhuri, Gavin Dunn, Eric Leuthardt, Hong Chen

Open Access Publications

No abstract provided.

Sarm1 Activation Produces Cadpr To Increase Intra-Axonal Ca++ And Promote Axon Degeneration In Pipn, Yihang Li, Maria F. Pazyra-Murphy, Daina Avizonis, Mariana De Sá Tavares Russo, Sophia Tang, Chiung-Ya Chen, Yi-Ping Hsueh, Johann S. Bergholz, Tao Jiang, Jean J. Zhao, Jian Zhu, Kwang Woo Ko, Jeffrey Milbrandt, Aaron Diantonio, Rosalind A. Segal

Open Access Publications

Cancer patients frequently develop chemotherapy-induced peripheral neuropathy (CIPN), a painful and long-lasting disorder with profound somatosensory deficits. There are no effective therapies to prevent or treat this disorder. Pathologically, CIPN is characterized by a "dying-back" axonopathy that begins at intra-epidermal nerve terminals of sensory neurons and progresses in a retrograde fashion. Calcium dysregulation constitutes a critical event in CIPN, but it is not known how chemotherapies such as paclitaxel alter intra-axonal calcium and cause degeneration. Here, we demonstrate that paclitaxel triggers Sarm1-dependent cADPR production in distal axons, promoting intra-axonal calcium flux from both intracellular and extracellular calcium stores. Genetic or …

The 2021 Faseb Science Research Conference On Nad Metabolism And Signaling, Vera Gorbunova, Shin-Ichiro Imai, Et Al

Open Access Publications

No abstract provided.

Epigenetic Regulation Of Human T‐Cell Leukemia Virus Gene Expression, Lee Ratner

Open Access Publications

No abstract provided.

Dopamine D4 Receptor Is A Regulator Of Morphine-Induced Plasticity In The Rat Dorsal Striatum, Alicia Rivera, Diana Suarez-Boomgard, Cristina Miguelez, Alejandra Valderrama-Carvajal, Jerome Baufreton, Kirill Shumilov, Anne Taupignon, Belen Gago, M.Angeles Real

Open Access Publications

No abstract provided.

Transition From Cmyc To L-Myc During Dendritic Cell Development Coordinated By Rising Levels Of Irf8, David A. Anderson, Feiya Ou, Sunkyung Kim, Theresa L. Murphy, Kenneth M. Murphy

Open Access Publications

During dendritic cell (DC) development, Myc expression in progenitors is replaced by Mycl in mature DCs, but when and how this transition occurs is unknown. We evaluated DC development using reporters for MYC, MYCL, and cell cycle proteins Geminin and CDT1 in wild-type and various mutant mice. For classical type 1 dendritic cells (cDC1s) and plasmacytoid DCs (pDCs), the transition occurred upon their initial specification from common dendritic cell progenitors (CDPs) or common lymphoid progenitors (CLPs), respectively. This transition required high levels of IRF8 and interaction with PU.1, suggesting the use of EICEs within Mycl enhancers. In pDCs, maximal MYCL …

M6a Modifications Regulate Intestinal Immunity And Rotavirus Infection, Anmin Wang, Gaopeng Hou, Siyuan Ding, Et Al

Open Access Publications

N6-methyladenosine (m6A) is an abundant mRNA modification and affects many biological processes. However, how m6A levels are regulated during physiological or pathological processes such as virus infections, and the in vivo function of m6A in the intestinal immune defense against virus infections are largely unknown. Here, we uncover a novel antiviral function of m6A modification during rotavirus (RV) infection in small bowel intestinal epithelial cells (IECs). We found that rotavirus infection induced global m6A modifications on mRNA transcripts by down-regulating the m6a eraser ALKBH5. Mice lacking the m6A writer enzymes METTL3 in IECs (

A Test Of The Pioneer Factor Hypothesis Using Ectopic Liver Gene Activation, Jeffrey L. Hansen, Kaiser J. Loell, Barak A. Cohen

Open Access Publications

The pioneer factor hypothesis (PFH) states that pioneer factors (PFs) are a subclass of transcription factors (TFs) that bind to and open inaccessible sites and then recruit non-pioneer factors (non-PFs) that activate batteries of silent genes. The PFH predicts that ectopic gene activation requires the sequential activity of qualitatively different TFs. We tested the PFH by expressing the endodermal PF FOXA1 and non-PF HNF4A in K562 lymphoblast cells. While co-expression of FOXA1 and HNF4A activated a burst of endoderm-specific gene expression, we found no evidence for a functional distinction between these two TFs. When expressed independently, both TFs bound and …

The Mutationathon Highlights The Importance Of Reaching Standardization In Estimates Of Pedigree-Based Germline Mutation Rates, Lucie A. Bergeron, Tychele Turner, Et Al

Open Access Publications

In the past decade, several studies have estimated the human per-generation germline mutation rate using large pedigrees. More recently, estimates for various nonhuman species have been published. However, methodological differences among studies in detecting germline mutations and estimating mutation rates make direct comparisons difficult. Here, we describe the many different steps involved in estimating pedigree-based mutation rates, including sampling, sequencing, mapping, variant calling, filtering, and appropriately accounting for false-positive and false-negative rates. For each step, we review the different methods and parameter choices that have been used in the recent literature. Additionally, we present the results from a 'Mutationathon,' a …

Intrinsic Mechanisms In The Gating Of Resurgent Na+ Currents, Joseph L. Ransdell, Jonathan D. Moreno, Druv Bhagavan, Jonathan R. Silva, Jeanne M. Nerbonne

Open Access Publications

The resurgent component of the voltage-gated sodium current (I