Digital Commons Network^™

Interspecies Tissue Transplants In Planarians, Maddie Thorstad

Student Presentations

Planarian flatworms have many stem cells called neoblasts which makes them adept at regenerating tissue after injury. This project aims to determine if neoblasts from two different species of planarians—Schmidtea mediterranea and Dugesia japonica—can integrate into the regenerated tissue of the other species. This is done by transplantation of a donor tissue plug from one species into a host planarian of the opposite species. After the host planarian is injured, neoblasts travel to the site of injury and begin regeneration of tissue. We have worked to optimize the transplantation procedure to visually obtain a 75% success rate. Simultaneously, we have …

Chemical Ablation Of Stem Cells, Renata Foerstel

Student Presentations

Planarians are an amazing model system for studying regeneration due to their large population of stem cells. A critical technique in studying stem cell dynamics is to eliminate stem cells through exposure to gamma or X-ray radiation. These techniques require expensive equipment. The goal of this project was to develop a cheap alternative to kill stem cells by using the cell cycle inhibitor hydroxyurea.

Functional Analysis Of Protonephridia Genes Identified By Drop-Seq, Andrew Gaetano

Student Presentations

Schmidtea mediterranea, the freshwater planarian, possesses protonephridia, which are an organized network of branched tubules that function in waste filtration and water regulation. On a cellular and molecular level, these structures show considerable homology to the human kidney making planarians a useful model system for exploring human excretory biology and disease (Scimone, 2011 and Vu, 2015). Additionally, the planarian genome contains many flatworm-specific protonephridia genes essential for survival, making them potential drug targets for parasitic worm infections of humans. We set out to validate the expression of protonephridia genes identified by Drop-seq, a single-cell RNA sequencing technique that identified the …

Analysis Of The Zebra Finch Gut Microbiota, Cole Hanke

Student Presentations

The purpose of this study is to analyze the gut microbiota of the zebra finch. The fecal samples of both male and female birds were collected from two separate populations. The bacteria were then incubated and isolated in order to amplify and sequence their 16s rRNA. The analysis of the 16s rRNA in a bacterial database showed the presence of a variety of species from the bacillus, staphylococcus, and citrobacter genera.

Genetic Characterization And Dna Barcoding Of The Zoonotic Parasite Gnathostoma, Ryan Pogodzinski

Student Presentations

Zoonotic diseases are transmitted to humans from wildlife and often pose serious health risks. One such disease, gnathostomiasis, is caused by parasitic nematodes in the genus Gnathostoma, commonly transmitted by consumption of infected undercooked freshwater fish. Lately, movement of animals over international borders has increased, leading to emerging zoonotic diseases (2018; CDC – One Health Basics). Human gnathostomiasis is just one example.

This project expands on previous research (Cole et al.) which identified Gnathostoma in invasive eels and imported eels from food markets, using morphological features and DNA sequences of the ribosomal RNA gene array’s ITS-2 (intergenic transcribed spacer-2) region. …

Mr. Vane’S Confessions: The Unexpected Play Of Augustine In Lilith’S Bibliographic Vision, Michael Wayne Wilhelm

North Wind: A Journal of George MacDonald Studies

No abstract provided.

Brigid Of Kildare: The Saint Who Got A Facelift, Aimee Hunt

Student Research

On the outskirts of Papal authority, early medieval Ireland created its own Christian identity separate from other European nations closer to Rome. Saint Brigid of Kildare, one of the patron saints of Ireland, played important yet problematic roles in that identity. After her death, the church began to alter her history. Being a female bishop, performing the first recorded abortion, and having both men and women within her monastery, Brigid had trodden on the male-dominated system in a way that few women had. Deemed unacceptable but having already been sainted, the Catholic church gave Brigid a holy facelift.

Repressive Interactions Between Transcription Factors Separate Different Embryonic Ectodermal Domains., Steven L. Klein, Andre L P Tavares, Meredith Peterson, Charles H Sullivan, Sally A. Moody

Anatomy and Regenerative Biology Faculty Publications

The embryonic ectoderm is composed of four domains: neural plate, neural crest, pre-placodal region (PPR) and epidermis. Their formation is initiated during early gastrulation by dorsal-ventral and anterior-posterior gradients of signaling factors that first divide the embryonic ectoderm into neural and non-neural domains. Next, the neural crest and PPR domains arise, either

Animated Short Film, Vinny Argentina

Summer Community of Scholars (RCEU and HCR) Project Proposals

No abstract provided.

Developing A Self-Navigating Robot To Map Disaster Regions, Nagavenkat Adurthi

Summer Community of Scholars (RCEU and HCR) Project Proposals

No abstract provided.

Informational Materials: Dual Prevention Pill, Population Council

Sexual and Reproductive Health, Rights, and Choices

Informational materials being used in a dual prevention pill study in South Africa.

Exploring The Effect Of Online Course Design On Preservice Teachers’ Knowledge Transfer And Retention Through Learning Analytics, Yasemin Gülbahar, Mohamed Ibrahim, Rebecca Callaway

Faculty Publications - Curriculum & Instruction

There is a vast amount of data collected on e-learning platforms that can provide insight and guidance to both learners and educators. However, this data is rarely used for evaluation and understanding the learning process. Hence, to fill this gap in the literature this study explored the effect of online course design on students’ transfer and retention of knowledge through learning analytics. The aim was to reveal study behaviours of participants over a short time while exploring their academic performance. Using a mixed method approach, this research is conducted in two different countries in a limited time. The results showed …

Trends And Outcomes Of A School-Based Campaign To Reduce Sugar-Sweetened Beverage Consumption Among Racially And Ethnically Diverse Students In Middle School, Roya Amirniroumand

Theses and Dissertations

Reducing sugar-sweetened beverage (SSB) consumption has been identified as a chronic disease prevention strategy in children and adolescents (Muth et al., 2019; USDA & HSS, 2015) and promoting healthier alternative beverage options has become an important focus for public health policy and educational efforts. Community-driven school-based health initiatives targeting SSB consumption have shown promising outcomes however, few studies have explored these strategies for middle school students and less is known about the potential benefits of these efforts with racial/ethnic minority youth.

The present study examined whether racial/ethnic differences in SSB consumption existed in a sample of 419 students at one …

Long-Term Stability Of Baseline Impact Scores In High School Football Players Across Age Groups, Christopher Thomas Burley

Theses and Dissertations

In recent decades, the increased awareness of the prevalence of concussions has resulted in significant advancements in concussion assessment and treatment, including annual or bi-annual pre-season computerized baseline assessments. However, limited research has focused on the differences in stability of baseline neurocognitive performance among different age groups in adolescence. The main purpose of the present study was to explore the long-term stability of ImPACT baseline assessments across one-year intervals in multiple age groups within a sample of high school football players. Subjects who completed two baseline assessments between the ages of 14 and 17 who had completed two baselines one …

A Greenhouse Gas Emissions Inventory For Nebraska: Livestock And Coal Loom Large, Eric R. Holley, Adam Liska

Adam Liska Papers

Mitigation of climate change requires the systematic identification and cataloging of emissions sources at city, state, and national levels. In this study, an inventory of annual greenhouse gas emissions from the state of Nebraska was created based on industry data, and emissions inventories were completed each year from 1990 to 2016. Nebraska’s net emissions were found to increase from 56.2 million metric tons of carbon dioxide equivalents (MMtCO₂e) in 1990 to 87.4 MMtCO₂e in 2016. Agriculture was found to be the sector with the most emissions (36 MMtCO₂e) followed by electricity generation (21 MMtCO …

Invasive Plants Watch List: 2022, Allison Zach

Nebraska Invasive Species Program

The purpose of the weed watch list is to collect data on the distribution of invasive plants found in various Nebraska counties. Counties were divided up into ‘ecoregions’ based on the Nebraska Game & Parks Commission’s Legacy Plan (map of regions below). The plants in the watch list have been identified based on their invasiveness in surrounding states and their increasing range in Nebraska. Data collected on watch list plant species distribution has been used to support the listing or delisting of noxious weeds. Plant species in the weed watch list are categorized based on early detection and rapid response …

Maximizing The Discovery Of Data Sets In The Yale University Library Catalog.”, Rowena Griem, Yukari Sugiyama, Tachtorn Meier

Library Staff Publications

Yale University Library’s Dataset Cataloging Task Force was formed in spring 2019 to address issues raised by the inclusion of data set holdings in the institutional catalog. The paper describes the group’s assessment of existing cataloging practices and the current integrated library system environment; the process of developing cataloging guidelines in the absence of authoritative resources to optimize the discoverability and accessibility of data sets in the online library catalog; and proposed next steps to discuss, establish, and document national guidelines for cataloging data sets so that these increasingly important resources are treated in a consistent manner in institutional, consortial, …

Artists’ Studio Archives Managing Your Studio Practice & Building A Creative Legacy Revised Edition (2022): A Comprehensive Guide For Artists & Their Assistants, Heather Gendron, Neal Ambrose-Smith, Joane E. Beaudoin, Eumie Imm-Stroukoff

Library Staff Publications

This guide is based on real-life scenarios and best practices in managing an art studio archive. It will aid artists in every career stage, from emerging artists to late-career artists; the yet-to- be-discovered and the well-established. Increasing productivity in the studio with good documentation helps artists better prepare to sell work and secure grant funding, as well as manage better the legal and financial aspects of an artistic practice.

Module 09: Introduction To Fusion 360 Part 1, Leendert Craig

ENTC 2170: Computer Aided Design and Drafting

No abstract provided.

Module 09: Introduction To Fusion 360 Part 1, Leendert Craig

ENGR 1110: Engineering Graphics

No abstract provided.

Comparison Of Phosphorus Removal And Recovery Methods And Proposed Design Of High Rate Algal Pond For Swine Wastewater, Taylor Fauth

Civil and Environmental Engineering Graduate Students Plan B Capstone Projects

High phosphorus concentrations in wastewater effluent can have harmful effects to aquatic environments and water quality. An excess of nutrients such as phosphorus in water bodies will cause eutrophication, which depletes the available oxygen within the water and is detrimental to aquatic life. Additionally, phosphorus leaching from wastewater discharge has negative impacts to groundwater quality. The depletion of phosphorus as a natural resource is also of concern as phosphorus is an essential product for agricultural and industrial production. Phosphorus is a non-renewable resource, and natural sources are becoming more difficult and expensive to access. Therefore, the removal and recycle of …

Idaho Rural Industry Clusters Needs Assessment 2021, Mcallister Hall, Benjamin Larsen, Aisha Kayed, Vanessa Fry

Idaho Policy Institute Reports

No abstract provided.

Seventh Annual Idaho Public Policy Survey, Matthew May, Lantz Mcginnis-Brown, Vanessa Fry

Idaho Policy Institute Reports

The Seventh Annual Idaho Public Policy Survey was conducted November 13-21, 2021, and surveyed 1,000 adults who currently live in Idaho. The sample is representative of the state’s population, both geographically and demographically, with a simple random sampling margin of error of +/- 3.1%. The survey covered a wide variety of topics, including growth, housing, transportation, state budgeting, taxes, education, the environment, and COVID-19. GS Strategy Group conducted the survey by cell phone (43%), landline phone (17%), online (30%), and text message (10%).

Transcriptomic Profiling Of Mice Brain Under Bex3 Regulation, Zhimin Xu, Noor Bahadar, Yingxin Zhang, Shuang Tan, Zhongze Wang, Bingyi Ren, Shitao Liu, Huanyan Dai, Yaowu Zheng, Bing Han

Turkish Journal of Biology

BEX family genes are expressed in various tissues and play significant roles in neuronal development. A mouse model of Bex3 gene knock-out was generated in this study, using the CRISPR-Cas9 system. Transcriptomic analysis of the brain was performed to identify genes and pathways under Bex3 regulation. Essential biological functions under the control of Bex3 related to brain development were identified. Ninety-five genes were differentially expressed under Bex3-/- regulation, with 53 down and 42 up. Among down-regulated genes, LOC102633156 is a member of zf-C2H2, Xlr3a is an X-linked lymphocyte regulated gene, LOC101056144 is a hippocampal related gene, 2210418O10Rik and Fam205a3 are …

Noncoding Rnas In Apoptosis: Identification And Function, Özge Tüncel, Merve Kara, Bi̇lge Yaylak, İpek Erdoğan, Bünyami̇n Akgül

Turkish Journal of Biology

Apoptosis is a vital cellular process that is critical for the maintenance of homeostasis in health and disease. The derailment of apoptotic mechanisms has severe consequences such as abnormal development, cancer, and neurodegenerative diseases. Thus, there exist complex regulatory mechanisms in eukaryotes to preserve the balance between cell growth and cell death. Initially, protein-coding genes were prioritized in the search for such regulatory macromolecules involved in the regulation of apoptosis. However, recent genome annotations and transcriptomics studies have uncovered a plethora of regulatory noncoding RNAs that have the ability to modulate not only apoptosis but also many other biochemical processes …

Sirna-Mediated Stathmin1 Silencing Inhibits Proliferation Of Prostate Carcinoma Cell Line, Asude Aksoy, Safi̇ye Selcen Vi̇cdanli, Ebru Önalan, Ahmet Tektemur, Gökhan Artaş, Asuman Orhan Varoğlu, Mustafa Koç

Turkish Journal of Biology

Stathmin1 (STMN1) has been proposed as a possible prognostic marker and a potential therapeutic target for some cancers. We aimed to analyze the changes in autophagy, invasion, apoptosis-related genes in prostate cancer (PCa) cell line (PC-3), after small interfering RNA (siRNA)-mediated STMN1 silencing, and also the relationships of STMN1 expression, clinicopathological parameters, and survival (OS) in PCa cases. The STMN1 expressions were analyzed, immunohistochemically, in formalin-fixed paraffin-embedded 75 PCa and 15 benign prostatic hypertrophy (BPH) tissues. The correlation between the levels of expression STMN1, clinicopathological features, and OS was determined in PCa cases. The siRNA-mediated STMN1 incubated PC-3 cells were …

Transcriptome Sequencing Reveals Circrna Expression Profile In Parkinson's Disease-Like Mice After Aerobic Exercise, Tianlun Fan, Xiating Li, Chuan Fu, Lichun Sun

Turkish Journal of Biology

Parkinson's disease (PD) is a common complex neurodegenerative disease, and aerobic exercise (EX) has potential to improve motor dysfunction. This study aimed to explore whether EX acts on PD in mice mode. Mice were administered 1-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine (MPTP) and subjected to a 4-week physical exercise regimen (EX-PD group) and underwent RNA-Seq. Here, MPTP caused PD, which was characterized by neuron shrinkage and behavioral deficits, whereas EX improved PD by rescuing neuronal survival and motor function in mice. Moreover, circRNA expression profiles identified a total of 142 differentially expressed circRNAs (DEcircRNAs) between PD and EX-PD group. These DEcircRNAs were mainly involved …

The Life History Of Euborellia Annulipes (Lucas) (Dermaptera: Anisolabididae) Fed Onlarvae And Pupae Of Plutella Xylostella (L.) (Lepidoptera: Plutellidae), Gilmar Da Silva Nunes, Thais Aparecida Vitoriano Dantas, Wennia Rafaelly Souza Figueiredo, Roberio De Oliveira, Jacinto De Luna Batista, Alessandra Marieli Vacari, Sergio Antonio De Bortoli

Turkish Journal of Zoology

Euborellia annulipes (Lucas) (Dermaptera: Anisolabididae) is reported as a potential predator of Plutella xylostella (L.) (Lepidoptera: Plutellidae). In this study, we aimed to investigate whether fourth instar larvae, 1 or 3-day-old pupae of P. xylostella are suitable food for the development and reproduction of E. annulipes. We evaluated the characteristics of the predation, development of each life stage, and reproduction of the predator, by comparing them with an artificial diet. The nymphs consumed larvae and pupae equally in the first instars, but, from the third to fifth, they consumed more larvae over pupae. Both males and females consumed more larvae …

High Temperatures Adversely Affect The Hoverfly Episyrphus Balteatus(Diptera: Syrphidae) Fitness And Aphid Prey Consumption, Gregoire Noel, Jessica Caetano, Solene Blanchard, Antoine Boullis, Frederic Francis

Turkish Journal of Zoology

Numerous studies have shown that an increase of environmental temperature could directly disturb insect larval development. Natural enemies of insect pests, particularly predators of aphid populations, are not spared. Following temperature predictions of Belgian climate change scenario, the goal of this study is to measure life-history traits, such as the mass, body length and aphid consumption (Acyrthosiphon pisum (Harris, 1776)) (Hemiptera: Aphididae) of Episyrphus balteatus (De Geer, 1776) (Diptera: Syrphidae) larvae under three constant temperature treatments (i.e. 20, 23, and 26 °C). We detected no significant differences between 20 °C and 23 °C on the hoverflies larval development, their aphidophagous …

Effect Of Urban Habitats On Colony Size Of Ants (Hymenoptera, Formicidae)In Memory Of Professor A. A. Zakharov (Russian Academy Of Sciences, Moscow), Stanislav Stukalyuk, Ascar Akhmedov, Alexey Gilev, Alexander Reshetov, Yuri Radchenko, Nataly Kosiuk

Turkish Journal of Zoology

Urbanized ecosystems are suitable for the habitat of only a few species of ants, due to conditions caused by human activities. Invasive species of ants have adapted to urbanized ecosystems most successfully. The study of the ant colonies sizes started in Crimea in 2013-2014. In 2019-2021 it was carried out in Ukraine (the Carpathians, Kyiv city, and Kyiv region), in Russia (Rostov-on-Don city and region, and the Urals), and in Uzbekistan (Tashkent city, and tugai forests). The study covers natural (forest, meadow, steppe), suburban (alleys and tree planting) and urban habitats (tree planting along streets and roads, botanical gardens). Our …