Digital Commons Network^™

Black Women Burdening: The Process Of "Unburdening" Realism In Dennis Mcintyre's Split Second., Brandi L. Threatt

Electronic Theses and Dissertations

Black women who dramatize reality can experience a transference of burdens if realistic plays reflect their lived experiences. Burdens affect truthful character development and impact the mental, emotional, physical, social and spiritual well-being of Black actresses. This thesis will use the Fall 2021 University of Louisville production of Dennis McIntyre’s Split Second as a case study. Gender and Race studies along with an auto-ethnographic research approach will be used to examine how my portrayal of Alea in Split Second contributed to Black Woman Burdening, a phrase I created to examine how Black fatigue can negatively and specifically affect Black women …

The Impact Of Volume Of Tissue Activation On Cortical-Striatal Networks And Verbal Fluency Declines In Post-Deep Brain Stimulation Parkinson's Disease Patients., Alexander Luke Alley

Electronic Theses and Dissertations

This study investigated the cortical-striatal networks of verbal fluency declines in 6-month, post-operative, deep brain stimulation Parkinson’s Disease patients. Nine Parkinson’s disease participants with implanted STN or GPi DBS systems were recruited for this study. Verbal fluency data was obtained from each patient preoperatively and 6- months post implantation. The stimulation-based volume of tissue activated area (VTA) of each target site (STN or GPi) was analyzed using Lead-DBS and Lead-Group. The white matter tracks intersecting each patient’s VTA, terminating in the pre-SMA, SMA, caudate nucleus, and anterior cingulate were investigated and correlated with verbal fluency declines. We found statistically significant …

Meat The Future., Xin Chen

Electronic Theses and Dissertations

This research focuses on the subtle relationship between human life, the environment, and other animals that we live among every day. Under the mainstream social background of capitalism and a commodity economy, animals are the victims of constant consumption and exploitation. However, meat is a basic substance for human nourishment, especially in certain cultures. As a result, meat becomes an important part of the commodity economy in capitalist societies, wherein the supply must be filled to accommodate society’s demand. This research formed an exhibition using glass and ceramic artwork using a playfully satirical tone to offer a crucial alternative: how …

Automatic Testing Of Organic Strain Gauge Tactile Sensors., Brian P. Goulet

Electronic Theses and Dissertations

Human-Robot Interaction is a developing field of science, that is posed to augment everything we do in life. Skin sensors that can detect touch, temperature, distance, and other physical interaction parameters at the human-robot interface are very important to enhancing the collaboration between humans and machines. As such, these sensors must be efficiently tested and characterized to give accurate feedback from the sensor to the robot. The objective of this work is to create a diversified software testing suite that removes as much human intervention as possible. The tests and methodology discussed here provide multiple realistic scenarios that the sensors …

Putting Colorism On Trial Intraracial Colorism And Its Impact On Crime Trajectory Among African Americans., Shaderica Ta'shawn Sibley

Electronic Theses and Dissertations

This study examines the impact of experiences of colorism on the crime trajectory of African Americans. It is qualitative in nature and utilizes semi-structured interviews with incarcerated African American people. As previous research has shown the painful impact of colorism on its victims, this study ultimately seeks to unearth if that pain translates into crime commission and trajectory. The primary purpose of this research is to contribute to the existing body of research that seeks to support “colorism healing” in the African American community. If this indicates that colorism contributes to crime trajectory, such an outcome would have practical implications: …

Acoustofluidic Delivery Of Gene Editing Compounds For Improved Immunotherapy Processing., Riyakumari K. Patel

Electronic Theses and Dissertations

Cell-based immunotherapies are a new generation of “living-drug” treatments for cancer and other diseases. Chimeric antigen receptor (CAR) T-cell cancer therapy has shown promising results in lymphoma and B-cell malignancies. Currently, there are six FDA-approved CAR-T drugs on the market, and all of them use viral transfection for reprogramming. While viral transfection is effective, there are safety concerns due to inconsistent transfection that limit the use of CAR-T therapy. Current non-viral transfection techniques generally have lower transfection efficiency than viral transfection. Additionally, these techniques can be toxic, time-consuming, non-transportable, and expensive. To address these limitations, a novel 3D printed acoustofluidic …

Using Stock And Flow Modeling To Address Knowledge Gaps In Marine Plastic Pollution Data, Daniel P. Martin

OES Theses and Dissertations

As plastic becomes a ubiquitous part of society, its growth outpaces waste disposal infrastructure and enters the environment as physical and chemical pollution. Plastic can also erode during the use cycle and reach the environment without any chance of being arrested by collection efforts. Plastic is a hazard to many parts of the earth’s life support system but there are many knowledge gaps regarding the processes by which plastic moves through the use cycle and environment. In particular, the ocean is generally regarded as a sink for plastic out of which it is difficult to escape, but plastic can sink …

Mycobacteriosis Of Striped Bass (Morone Saxatilis) In Virginia Tributaries Of The Chesapeake Bay, Joshua S. Mcgilly

Biological Sciences Theses & Dissertations

Mycobacteriosis was first documented in Chesapeake Bay striped bass (Morone saxatilis) in 1997 after fish exhibited emaciation and skin lesions. Since it was first identified, studies of mycobacteriosis in the mainstem of the Chesapeake Bay (2003-2007) and the Rappahannock River (2005-2012) have shown high disease prevalence and disease-associated mortality. Until this study, no current prevalence data existed from the Rappahannock River, and no published prevalence data existed for the James River, leaving a gap in our understanding of this disease in major Chesapeake Bay tributaries. We began gathering mycobacteriosis prevalence data from an existing survey collecting striped bass …

Di•As•Po•Ra: Displaced, Not Erased., Betty Álvarez

Electronic Theses and Dissertations

This thesis is based on my artistic endeavors and research founded in my culture, family, the history of the United States government’s intervention in El Salvador, and the current political climate Latin Americans face today in the United States. I work within multiple mediums to resurrect pieces of my culture that are being forgotten or left behind due to assimilation. I accomplish this through revisiting and reinterpreting traditions. I aim to bring awareness of the Latin experience in a racially divided United States through artistic expression and my own personal experiences. My works are made to feel whimsical and playful, …

Dental Morphology In A Juvenile Mammal From The Late Cretaceous Of South America: Tooth Eruption And Development., Kayla E Newton

Electronic Theses and Dissertations

Meridiolestids are a diverse and distinct clade of Cretaceous–Miocene South American dryolestoid mammals ranging in size from small shrew-sized insectivores to large dog- sized omnivores. Cronopio dentiacutus from the Cenomanian La Buitrera locality, Rio Negro Province, Argentina (Candeleros Fm.) is the oldest and best known of them, represented by skull material and associated jaws. A juvenile skull as attributed to Cronopio dentiacutus, based on tooth size and morphology, was collected from the type locality. The specimen includes the skull and both articulated lower jaws. The fossil is poorly ossified and partially flattened; CT scanning reveals most of the dentition …

Alleviation Of Atopic Dermatitis Symptoms By Implementation Of Standard And Alternative Treatments, Nika Sayvongsa

The Eleanor Mann School of Nursing Undergraduate Honors Theses

Atopic Dermatitis (AD) is a chronic dermatological disease caused by a combination of hereditary and environmental factors. This is a common condition that is primarily diagnosed among children, but can persist throughout adulthood. Twenty peer-reviewed articles are analyzed in this expanded literature review. Various methods were implemented throughout these studies, including randomized, double-blind, placebo-controlled, and single-blind studies; all of the studies shared the objective of evaluating AD alleviation with the use of either standard treatment modalities or alternative/ complementary therapies.

Teaching Empathy: A Precursor To Accepting Diversity, Karen Viers

Undergraduate Honors Theses

Robert Kennedy once said, “The purpose of life is to contribute in some way to making things better.” This statement inspired the author to question how prosocial behavior and the acceptance of diversity can be encouraged and is the driving force behind the following undergraduate thesis. Research tells us that Theory of Mind (ToM) and Affective Perspective Taking (APT) are essential components in a stepwise progression to learning empathy and can be taught to children ages five to seven. These elements are essential to developing an empathetic foundation that leads to healthier relationships, reduces bullying, and encourages the acceptance of …

Simulating Polistes Dominulus Nest-Building Heuristics With Deterministic And Markovian Properties, Benjamin Pottinger

Undergraduate Honors Theses

European Paper Wasps (Polistes dominula) are social insects that build round, symmetrical nests. Current models indicate that these wasps develop colonies by following simple heuristics based on nest stimuli. Computer simulations can model wasp behavior to imitate natural nest building. This research investigated various building heuristics through a novel Markov-based simulation. The simulation used a hexagonal grid to build cells based on the building rule supplied to the agent. Nest data was compared with natural data and through visual inspection. Larger nests were found to be less compact for the rules simulated.

Covid-19 In Tennessee: Lessons Learned And A Tool For The Future, Allen Archer, Randy Wykoff, Megan Quinn

Undergraduate Honors Theses

Background: In late 2019, the SARS-CoV-2 virus was identified in Wuhan, China. Within a matter of weeks, the virus spread to the United States and many other countries around the world. By March 5, 2020, the first case of COVID-19 (the disease caused by the SARS-CoV-2 virus) was identified in the state of Tennessee, and the first known COVID-19 associated death in the state followed on March 20, 2020. In 2020 alone, there were 514,922 confirmed cases and 6,760 COVID-19 attributed deaths in the state of Tennessee. It is important to understand, however, that confirmed COVID-19 deaths do not accurately …

Resisting Face Surveillance With Copyright Law, Amanda Levendowski

Georgetown Law Faculty Publications and Other Works

Face surveillance is animated by deep-rooted demographic and deployment biases that endanger marginalized communities and threaten the privacy of all. But current approaches have not prevented its adoption by law enforcement. Some companies have offered voluntary moratoria on selling the technology, leaving many others to fill in the gaps. Legislators have enacted regulatory oversight at the state and city levels, but a federal ban remains elusive. Both approaches require vast shifts in practical and political will, each with drawbacks. While we wait, face surveillance persists. This Article suggests a new possibility: face surveillance is fueled by unauthorized copies and reproductions …

Targeting Neuronal Nitric Oxide Synthase (Nnos) For Melanoma Treatment, Shirley Tong

Pharmaceutical Sciences (PhD) Dissertations

Human cutaneous melanoma is the most aggressive form of skin cancer and the incidence rates have continued to increase over the years. Neuronal nitric oxide synthase (nNOS) produces nitric oxide (NO) has been found to be overexpressed in human melanoma and the expression of nNOS is induced by interferon-gamma (IFN-γ). In our studies, nNOS has been implicated in IFN-γ-stimulated melanoma progression and the inhibition of nNOS using novel inhibitors effectively inhibited IFN-γ-stimulated tumor growth in a xenograft mouse model. Programmed death-ligand 1 (PD-L1) is overexpressed in melanoma and plays an important role in suppressing the immune system ^12-14. Our …

Content And Activity Of Cytochrome P450 3a In Rat Brain Microsomes And Mitochondria, Nouf Alshammari

Pharmaceutical Sciences (MS) Theses

The potential contribution of CYP3A in brain microsomes and mitochondria is crucial to designing appropriate therapeutic strategies and preventing toxicities. Until recently, there has been limited data reported in the literature with respect to CYP3A activity and its expression in the brain tissue. The reason for this limitation is most likely the relatively low level of CYP3A in the brain tissue. To this end, the primary purpose of this study is to develop a CYP3A functional assay in rat brain mitochondria and microsomes to determine the Michaelis-Menten kinetics of the formation of CYP3A-mediated hydroxylated metabolites of midazolam by UPLC-MS/MS. We …

Investigating Hybrid Cyclic/Linear And Linear Peptides As Vehicles For Nucleic Acid Delivery, Abdulelah Alhazza

Pharmaceutical Sciences (MS) Theses

Small interfering RNA (siRNA) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated Protein 9 (Cas9) are advanced biotechnological approaches that have been able to temporarily downregulate gene expression through post-transcription RNA interference or permanently knockdown a protein via creating a mismatch in DNA, respectively. Nucleic acids are anionic and hydrophilic molecule, which cannot enter the cell through passive diffusion and/or interaction with the cell membrane. Cell-penetrating peptides (CPPs) offer a unique carrier for nucleic acid delivery, where positively charged components of CPP bind to the negatively charged nucleic acid and the hydrophobic components enhance the interaction with cell membrane, and …

Islamic Bioethics: National Regulations And Guidelines Of Human Stem Cell Research In The Muslim World, Azza Mahmoud

International Studies (MA) Theses

The utilization of human stem cells emerged recently in the Muslim world as one of the essential valuable areas of medicine for their vital role in developing regenerative medicine and treating chronic and incurable diseases. Existing studies indicate that most human stem cell researchers rely on varying schools of thought in Islamic law or on an individual base to define legitimate practices. From a policy perspective, the different Islamic religious decrees do not constitute a unified legal framework to promote essential international collaborations. The existing literature exhibits a limitation in comprehensive studies on human stem cell research (HSCR) in the …

Studying The Role Of Cerebrovascular Changes In Different Compartments In Human Brains In Hypertension Prediction, Heba Kandil, Ahmed Soliman, Nada Elsaid, Ahmed Saied, Norah Saleh Alghamdi, Ali Mahmoud, Fatma Taher, Ayman El-Baz

All Works

Hypertension is a major cause of mortality of millions of people worldwide. Cerebral vascular changes are clinically observed to precede the onset of hypertension. The early detection and quantification of these cerebral changes would help greatly in the early prediction of the disease. Hence, preparing appropriate medical plans to avoid the disease and mitigate any adverse events. This study aims to investigate whether studying the cerebral changes in specific regions of human brains (specifically, the anterior, and the posterior compartments) separately, would increase the accuracy of hypertension prediction compared to studying the vascular changes occurring over the entire brain’s vasculature. …

An Investigation Towards The Synthesis Of A Novel Conformationally Restricted Ethylenediamine Scaffold, Fanny Mai

Theses, Dissertations and Culminating Projects

This study reports the synthesis of a novel conformationally restricted ethylenediamine scaffold that can be explored for drug discovery. There was significant progress in synthesizing the target scaffolds, but future studies are needed to finish this synthesis. This potential ethylenediamine compound may offer new scaffolds for exploration in drug design and orexin receptor antagonists with improved selectivity for OX1R and OX2R.

Gender Roles In Natural Resource Use In Madagascar, Sarahana Shrestha

Theses, Dissertations and Culminating Projects

Understanding the role that gender plays in natural resource use is crucial for effective conservation efforts. While Madagascar is a priority for international conservation due to its high levels of endemism and unsustainable natural resource use, there is little information on how gender roles affect forest product use. This study uses over seven years of data on gender roles within the extraction, use, and sale of natural forest and marine resources near Madagascar’s largest national park to inform future conservation strategies. We found that gender significantly affects how one uses natural resources in Madagascar. Men were primarily responsible for the …

Effect Of Soil Conditions On Invasive Purple Loosestrife Compared To Native Species, Madeline Ruth Baum

Theses, Dissertations and Culminating Projects

Purple loosestrife (Lythrum salicaria) is an invasive plant that has degraded many wetland habitats since its initial introduction to the United States in the early 19th century. To aid in managing purple loosestrife, it is important to study how purple loosestrife grows under variable environmental conditions compared to common native species, which can help managers understand which habitats are most at-risk of invasion. High or low salinity and pH are often barriers to plant growth, and the stress caused by these environmental conditions can cause plants to deplete their starch reserves. If native species are more negatively affected by stressful …

“Don’T Call Me A Crouton” : A Case Study On The Use Of Humor In Organizational Relationships On Twitter And Facebook, Hannah Grinberg

Theses, Dissertations and Culminating Projects

An organization using humor on social media can potentially engage in dialogue through participatory boundary-work that creates an in-group between themselves and their online user publics. The ability for an organization to use humor to form that in-group, where dialogic communication is achievable, is based on the organization’s ability to produce an instance of shared mirth with their online publics. The shared experience of mirth not only determines if the organization reaches shared meaning with their receiver publics, but also indicates who the humor is intended for, and who will be left out of the in-group created. To determine the …

A Geochemical And Mineralogical Evaluation Of The Mona Lisa Turquoise Mine, Polk County, Arkansas, Alexander Arai Goodsuhm

Graduate Theses and Dissertations

The presence of turquoise in Arkansas has long been disputed by geologists and gem collectors. Despite the prevalence of other phosphate minerals in Arkansas, such as planerite, wavellite, and variscite, the absence of an obvious copper (Cu) source required for turquoise formation was considered too great of an obstacle for turquoise mineralization. In this study, geologic, mineralogical, and geochemical techniques provide overwhelming evidence that the novaculite-hosted Mona Lisa Mine in Arkansas does produce turquoise. X-ray diffraction and Raman spectroscopy have confirmed the structural nature of the material and identified other phosphate minerals, such as crandallite, often found in assemblage with …

To The Window, To The Wall: Improving Environmental Monitoring Protocols, Sarah Jones

Graduate Theses and Dissertations

Detecting microorganisms on environmental surfaces via an environmental monitoring (EM) program is part of a preventive food safety culture. Environmental monitoring should 1) verify that food safety plans are reducing cross-contamination risk from surfaces to food, 2) pinpoint microbial niches, and 3) prevent the transmission of pathogens. Environmental monitoring programs utilize EM tools, such as sponges, to sample food contact and non-food contact surfaces. However, EM tool selection is determined by the individual food firm. This dissertation evaluated and characterized factors influencing EM program effectiveness in the food industry. Specifically, this dissertation focuses on the release of microorganisms from EM …

Examination Of The Colorful Semantic Approach Via Telepractice For Children Who Are Deaf Or Hard Of Hearing, Samadhi Pusuba Devayalage

Graduate Theses and Dissertations

Introduction: Hearing loss, deafness or hard of hearing are considered to be the inabilityof perceiving sounds beyond 20 dB. Due to a direct impact of a hearing loss, a developing brain undergoes difficulties in acquiring age-appropriate syntax and speech sounds. As a result, children with hearing loss present language, speech, and literacy disabilities. The current study discusses the efficacy of the colorful semantics approach in order to see its impact on sentence structure development. Methodology: A single subject withdrawal experimental study conducted following ABAB model. Two participants (6 years and 10 years) were recruited to the study following an inclusion …

Archivos Del Fracaso: La Escritura De La Memoria Histórica En Cuatro Novelas Documentales Latinoamericanas Del Siglo Xxi, Vanessa Guerrero

Graduate Theses and Dissertations

This doctoral dissertation aims to study four Latin American documentary novels of the 21st century. This type of novel is especially characterized by its deep and direct connection with historical archives, its high degree of auto fictionality and the self-awareness elaboration of an extreme ambiguity between fictionality and factuality. Likewise, this research proposes a reading of this type of novels through what has been called the poetics of failure, which configures not only the defeat of the characters within the plot, but also offers an ethical and aesthetic position in order to represent historical memory in a different way.

In …

Introduction Of Human Acidic Fibroblast Growth Factor (Fgf1) Variant With Increased Stability And Bioactivity, Azadeh Tavousi Tabatabaei

Graduate Theses and Dissertations

Human acidic Fibroblast Growth Factor 1 (FGF-1) involves in a broad spectrum of biological processes, including cell growth, proliferation, differentiation, migration, angiogenesis, wound healing, and embryonic development. hFGF1 non-selectively binds to cell surface hFGF receptor isoforms to elicit these cell-signaling processes. Since hFGF1 plays a significant role in tissue repair activity, that is a prime candidate for novel wound healing therapeutics. However, hFGF1 has been found to unfold near physiological temperature due to a strong electrostatic repulsion created by a dense cluster of positively charged amino acids near the c-terminus. The problem not only leads to proteolytic degradation of the …

Optimizing Virus Prefiltration For Biopharmaceutical Manufacturing, Solomon Isu

Graduate Theses and Dissertations

Virus filters are single-use devices that use a size-based separation process. In virus filters, contaminating virus particles are retained while the therapeutic molecules pass through the membrane pores. Virus filters are an essential component of the overall virus clearance strategy. Sections 1 and 2 of this dissertation provide an introduction and extensive review of monoclonal antibody (mAb) process development, where virus filtration is pivotal. In section 3, prefiltration studies were performed with an industrially relevant IgG1 type mAb using adsorptive and size-exclusion-based prefilters with different mechanisms of action. This mAb has an isoelectric point range of 7.1 to 8.0 and …