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Biology

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Biol 337-002 Collective Intelligence In Biological Systems, Simon Garnier Jan 2024

Biol 337-002 Collective Intelligence In Biological Systems, Simon Garnier

Biology Syllabi

No abstract provided.


Biol 340-102, 104: Mammalian Physiology, Eric Fortune Jan 2024

Biol 340-102, 104: Mammalian Physiology, Eric Fortune

Biology Syllabi

No abstract provided.


Biol 315-002: Principles Of Neurobiology, Kristen Severi Jan 2024

Biol 315-002: Principles Of Neurobiology, Kristen Severi

Biology Syllabi

No abstract provided.


Biol 606-102: Applied Bioprocessing & Immunology Based Therapies, John Yarotsky Jan 2024

Biol 606-102: Applied Bioprocessing & Immunology Based Therapies, John Yarotsky

Biology Syllabi

No abstract provided.


Biol 475-H02, Maria Stanko Jan 2024

Biol 475-H02, Maria Stanko

Biology Syllabi

No abstract provided.


Biol 646-002: Endocrinology, John Yarotsky Jan 2024

Biol 646-002: Endocrinology, John Yarotsky

Biology Syllabi

No abstract provided.


Biol 150-004: Living In A Variable Universe, Eric Fortune Jan 2024

Biol 150-004: Living In A Variable Universe, Eric Fortune

Biology Syllabi

No abstract provided.


Biol 462-002: Comparative Biomechanics, Brooke Flammang Jan 2024

Biol 462-002: Comparative Biomechanics, Brooke Flammang

Biology Syllabi

No abstract provided.


Biol 628-002: Cell Biology Of Disease, Darshan Desai Jan 2024

Biol 628-002: Cell Biology Of Disease, Darshan Desai

Biology Syllabi

No abstract provided.


Biol 375-002: Conservation Biology, Xiaonan Tai Jan 2024

Biol 375-002: Conservation Biology, Xiaonan Tai

Biology Syllabi

No abstract provided.


Biol 447-Hm2 Systems Neurobiology, Farzan Nadim Jan 2024

Biol 447-Hm2 Systems Neurobiology, Farzan Nadim

Biology Syllabi

No abstract provided.


Biol 205-002, Foundations Of Biology: Ecology And Evolution, Caroline Devan Jan 2024

Biol 205-002, Foundations Of Biology: Ecology And Evolution, Caroline Devan

Biology Syllabi

No abstract provided.


Biol 201-002: Foundations Of Biology: Cell & Molecular Biology, Mary Konsolaki Jan 2024

Biol 201-002: Foundations Of Biology: Cell & Molecular Biology, Mary Konsolaki

Biology Syllabi

No abstract provided.


Differential Photosynthetic Yield And Accumulated Degree Day Estimation For Parrotfeather (Myriophyllum Aquaticum) To Determine Invasive Potential In The Midwest, United States, Alyssa Anderson Jan 2024

Differential Photosynthetic Yield And Accumulated Degree Day Estimation For Parrotfeather (Myriophyllum Aquaticum) To Determine Invasive Potential In The Midwest, United States, Alyssa Anderson

All Graduate Theses, Dissertations, and Other Capstone Projects

Thank you to the Aquatic Plant Management Society (APMS) and affiliated chapters for funding this project. I would like to thank Mississippi State University for sending my source parrotfeather for propagation throughout the course of this project. Thank you to Jenna Eklund, Allison Squires, Thomas Zellmer, Justin Kjorness, and others in the Weed Science lab for their assistance. Finally, I would like to extend my thanks and gratitude to my committee members: Ryan Wersal, PhD, Christopher Ruhland, PhD, and Gray Turnage, PhD for their everlasting support and guidance throughout this project.


How Does A Global Climate Cycle Affect The Reproductive Output Of A Neotropical Migratory Bird?, Abby K. Bressette, Emma Cummings, Daniel Albrecht-Mallinger, Lesley Bulluck Jan 2024

How Does A Global Climate Cycle Affect The Reproductive Output Of A Neotropical Migratory Bird?, Abby K. Bressette, Emma Cummings, Daniel Albrecht-Mallinger, Lesley Bulluck

Undergraduate Research Posters

El-Niño Southern Oscillation (ENSO) causes regional changes in climate and has been found to have varying effects on the survival, fecundity, and recruitment of migratory songbirds. This study seeks to better understand the effect of ENSO on the fecundity of Virginia-breeding Prothonotary warbler (Protonotaria citrea; PROW), a neotropical migratory songbird. PROW breed in the Eastern United States and spend the non-breeding season primarily in Panama and Colombia. Ongoing research in Virginia has found a positive relationship between spring precipitation and predicted number of PROW fledgelings during La Niña, but the opposite during El Niño. To better understand this relationship, we …


Wildar Northeast Florida, Kailan Sindelar Jan 2024

Wildar Northeast Florida, Kailan Sindelar

UNF Faculty Research and Scholarship

Learn about some of the unique aspects of life in the wetlands and rivers of Northeast Florida and how we study them. In this app players see the memories of a student at the University of North Florida play out in front of them in AR. This app was created with funding the Digital Humanities Institute at the University of North Florida and informed by subject matter experts at the university.


Localization And Quantification Of Atpase, Ca2+ Transporting, Plasma Membrane 2 (Atp2b2) In The Developing Euthyroid And Hypothyroid Cochlea, Emily Gregersen Jan 2024

Localization And Quantification Of Atpase, Ca2+ Transporting, Plasma Membrane 2 (Atp2b2) In The Developing Euthyroid And Hypothyroid Cochlea, Emily Gregersen

All Graduate Theses, Dissertations, and Other Capstone Projects

Thyroid hormone (TH) is essential for auditory system development. Proper auditory function requires remodeling and transformation of cochlear structures during a critical developmental period. In rodent models, low TH during this timeframe delays remodeling and impacts ion channel function required for hearing to occur. TH receptors are ligand-dependent transcription factors that regulate expression of genes. Considering this, auditory deficits observed in hypothyroidism are thought to be largely the result of altered expression of specific TH receptor target genes. TH is reported to impact calcium signaling in several tissues, but whether regulators of calcium signaling are altered following developmental hypothyroidism is …


What A Waste: Nitrogen Runoff And Rates In The Maumee River (Ohio, Usa), Ryanne Antonette Cimatu Jan 2024

What A Waste: Nitrogen Runoff And Rates In The Maumee River (Ohio, Usa), Ryanne Antonette Cimatu

Browse all Theses and Dissertations

Excess anthropogenic nitrogen (N), primarily from agricultural field fertilization, causes nutrient runoff that stimulates harmful algal blooms (HABs) in western Lake Erie. As a critical tributary to Lake Erie, nutrient loading from the Maumee River drives the intensity of the annual summer HABs in the western basin. Knowledge gaps around rates of N transformations in the Maumee River currently hinder the calibration of in-river parameters in Soil and Water Assessment Tool (SWAT) models for the Maumee watershed. To address these gaps, this research quantified rates of ammonium uptake, ammonium remineralization, nitrification, and bacterial respiration alongside physicochemical parameters of the river. …


Research Into The Effects Of Invasive Earthworm Species, Elizabeth Gillis Jan 2024

Research Into The Effects Of Invasive Earthworm Species, Elizabeth Gillis

Williams Honors College, Honors Research Projects

Amynthas gracilis, or Alabama jumper worms, are a species of earthworms invasive to North America that hail from Asia. These worms are known to have an effect on the levels of macro-nutrients in the soil, as well as make alterations to the structure of the soil. These changes to soil composition and structure alter the soil’s ability to retain moisture and have been shown to negatively impact European species of earthworms such as Dutch Reds (Eisenia hortensis), Red Wigglers (Eisenia fetida,) and Canadian Nightcrawlers (Lumbricus terrestris). These effects were observed and quantified by …


Potential Role Of Ttt Complex In Regulating Dna Replication Checkpoint In The Fission Yeast Schizosaccharomyces Pombe, Sankhadip Bhadra Jan 2024

Potential Role Of Ttt Complex In Regulating Dna Replication Checkpoint In The Fission Yeast Schizosaccharomyces Pombe, Sankhadip Bhadra

Browse all Theses and Dissertations

DNA replication can be perturbed by various agents that slow or stall the replication forks, causing replication stress. If undetected, stressed forks may collapse, causing mutagenic DNA damage or cell death. In response to replication stress and DNA damage, the eukaryotic cell activates the DNA replication checkpoint (DRC) and DNA damage checkpoint (DDC) pathways to promote DNA synthesis, repair, and cell survival. The two cell cycle checkpoint pathways are controlled by the protein sensor kinases Rad3 (hATR/scMec1) and Tel1 (hATM/scTel1) in fission yeast, although Tel1 plays a minimal role in checkpoint functions. Rad3 and Tel1 belong to a family of …


Investigating Calcium Levels During Excitation-Contraction Coupling In Model Huntington's Disease Skeletal Muscle, Pooneh Hajmirza Mohammadi Kamalabadi Jan 2024

Investigating Calcium Levels During Excitation-Contraction Coupling In Model Huntington's Disease Skeletal Muscle, Pooneh Hajmirza Mohammadi Kamalabadi

Browse all Theses and Dissertations

One of the less studied aspects of Huntington’s disease (HD) is its impact on skeletal muscle function. Recent studies have suggested that HD patients exhibit altered muscle function and reduced muscle strength, which may be related to defects in Ca2+ handling during excitation-contraction coupling (ECC). However, the underlying mechanisms of these alterations remain poorly understood. This study aims to determine if the myoplasmic Ca2+ during ECC is reduced in late-stage R6/2 (mouse model of HD) muscle fibers compared to age-matched controls during twitch or tetanic stimulation. Such a decrease would help explain weakness or fatigue in the model HD muscle. …


Investigating The Effects Of Human Astroviruses On Aquaporins And Tight Junction Proteins In Vitro, Makalia T. Robinson Jan 2024

Investigating The Effects Of Human Astroviruses On Aquaporins And Tight Junction Proteins In Vitro, Makalia T. Robinson

Browse all Theses and Dissertations

Human Astroviruses (HAstV) are positive sense non-enveloped single stranded RNA viruses. HAstV is divided into classical (serotype 1-8) and non-classical (HAstVMLB 1-3 and HAstV-VA1-5) astroviruses. Classical HAstV is known to cause diarrhea in children, elderly, and immunocompromised patients. Additionally, the etiology of nonclassical HAstV remains unknown. However, recent studies have implicated non-classical HAstV closely associated with immunocompromised patients diagnosed neurological diseases like acute encephalitis. The mechanisms by which HAstV cause diarrhea is currently unknown. Aquaporins (AQPs) are integral membrane proteins that regulate the movement of water and small uncharged molecules across the cell membrane in response to osmotic gradients. Tight …


Nsp3 Protein-Portion Interaction Of Sars-Cov-2 Using Two Yeast-Hybrid System, Ghadeer Jassem Zakareya Jan 2024

Nsp3 Protein-Portion Interaction Of Sars-Cov-2 Using Two Yeast-Hybrid System, Ghadeer Jassem Zakareya

Selected Full-Text Master Theses 2021-

The NSP3 protein of SARS-CoV-2 plays a crucial role in the virus-host interaction during infection. This thesis explores the protein-protein interactions between NSP3 and host factors, shedding light on their significance in viral replication and pathogenesis. NSP3 is known to interact with various host proteins involved in key cellular processes, including innate immune response modulation, RNA processing and protein degradation pathways. Understanding these interactions is essential for developing targeted therapeutic strategies against COVID-19. Application of The Matchmaker GAL4Two-Hybrid Systems, to isolate the NSP3 protein and study the interactions, elucidating the NSP3 host protein interactions and attempting to identify potential key …


Softening And Cross-Susceptibility: Exposure To Heat And Desiccation Reduces Future Stress Tolerance In An Insect, Lauren Harter Jan 2024

Softening And Cross-Susceptibility: Exposure To Heat And Desiccation Reduces Future Stress Tolerance In An Insect, Lauren Harter

University of the Pacific Theses and Dissertations

The frequency and duration of environmental stressors, such as heat waves and drought, will continue to grow due to ongoing climate change, thereby increasing the likelihood that organisms will experience stressors consecutively. Exposure to one stressor can improve or impair future tolerance to the same stressor (i.e., hardening or softening, respectively), or enhance or reduce future tolerance to a different stressor (i.e., cross-protection or cross-susceptibility, respectively). Understanding whether stress improves or impairs animals’ abilities to withstand future stressors is critical for determining the physiological sensitivity of animals to ongoing climate change. Here, I evaluated the responses to consecutive heat and …


Carry-Over Effects Of Climate On Prothonotary Warbler (Protonotaria Citrea) Feather Quality, Alyssa N. Spasic, Dan Albrecht-Mallinger, Lesley Bulluck Jan 2024

Carry-Over Effects Of Climate On Prothonotary Warbler (Protonotaria Citrea) Feather Quality, Alyssa N. Spasic, Dan Albrecht-Mallinger, Lesley Bulluck

Undergraduate Research Posters

Avian species have inspired numerous studies on ornamentation, exploring its role in intra- and intersexual selection as a marker for individual quality. Male birds have been the focus of most of these studies while females, which can also display ornamentation, have been studied less. Coloration achieved through molt, particularly carotenoid-based pigments, requires energy derived from food resources. As climate affects insect abundance, there may be a link between climate conditions during molt and feather quality the following season. We assessed the relationship between temperature and precipitation during molt and female Prothonotary Warbler (Protonotaria citrea) breast and crown feather reflectance metrics …


A Comparison Of Three Dna Extraction Methods On 45-Year-Old Pupae Cases, Ananya Udyaver, Casey Flint, Jeffery Tomberlin, Baneshwar Singh Jan 2024

A Comparison Of Three Dna Extraction Methods On 45-Year-Old Pupae Cases, Ananya Udyaver, Casey Flint, Jeffery Tomberlin, Baneshwar Singh

Undergraduate Research Posters

Blow flies are the primary colonizers of human cadavers. In many death investigations, insect evidence can help in prediction of minimum post-mortem interval (PMImin) but to do that, the first step is to identify collected insect evidence. For immature stages, morphological keys are either limited or incomplete and hence DNA based identification is used. For DNA based ID, DNA extraction is the first and the most important step, especially when collected evidence is highly degraded. The main aim of this study was to compare three DNA extraction methods (QIAgen Blood and Tissue Kit, organic, and QIAgen DNA Investigator Kit) to …


Quantitative Shotgun Proteomic Analysis Of Bacteria After Overexpression Of Recombinant Spider Miniature Spidrion, Masp1, Kathryn P. Randene Jan 2024

Quantitative Shotgun Proteomic Analysis Of Bacteria After Overexpression Of Recombinant Spider Miniature Spidrion, Masp1, Kathryn P. Randene

University of the Pacific Theses and Dissertations

Spider silk has extraordinary mechanical properties, displaying high tensile strength, elasticity, and toughness. Given the high performance of natural fibers, one of the long-term goals of the silk community is to manufacture large-scale synthetic spider silk. This process requires vast quantities of recombinant proteins for wet-spinning applications. Attempts to synthesize large amounts of native size recombinant spidroins in diverse cell types have been unsuccessful. In these studies, we design and express recombinant miniature black widow (Latrodectus hesperus) MaSp1 spidroins in bacteria that incorporate the NTD and CTD, along with varying numbers of codon-optimized internal block repeats. Following spidroin overexpression, we …


Effects Of Glyphosate-Based Herbicide Exposure On Life History, Stress Tolerance, And Microbiome—From Plasticity To Evolution, Jacob R. Whitlock Jan 2024

Effects Of Glyphosate-Based Herbicide Exposure On Life History, Stress Tolerance, And Microbiome—From Plasticity To Evolution, Jacob R. Whitlock

University of the Pacific Theses and Dissertations

Glyphosate (GLY) is the most used pesticide worldwide, and GLY-based herbicides (GBHs) can affect animals’ microbiomes and, in turn, their physiology and morphology. Environmental stressors can be mitigated by flexibility in life-history strategies, which can also dictate animal physiology and morphology. However, we lack an understanding of how GBH influences life-history strategies and stress tolerance, in addition to its effects across generations. Thus, I exposed the variable field cricket, Gryllus lineaticeps, to GBH within- and across-generations to examine the role of GBH in developmental plasticity and evolution. Specifically, I measured its effects on life-history traits (e.g., developmental duration and a …


Effects Of Fetal Bovine Serum On The Proteome And Secretome Of Pichia Pastoris, Kenneth L. Nguyen Jan 2024

Effects Of Fetal Bovine Serum On The Proteome And Secretome Of Pichia Pastoris, Kenneth L. Nguyen

University of the Pacific Theses and Dissertations

Komagataella pastoris, formerly known as Pichia pastoris, and hereafter referred to as Pichia, is a methylotrophic yeast widely employed as a recombinant protein factory for biotechnical and industrial purposes. P. pastoris boasts the ability to thrive at high cell densities, executes numerous post-translational modifications, and exhibits minimal secretion of endogenous proteins, thus greatly facilitating the expression and purification of recombinant proteins. Despite these advantages, Pichia still presents certain challenges as an expression system. Occasionally, recombinant proteins are retained within the cell and subject to degradation. Furthermore, Pichia falls short in matching the production capabilities of more widely used systems like …


The Role Of The Unfolded Protein Response In Fatty Liver Disease, Anusha L. Murshed Jan 2024

The Role Of The Unfolded Protein Response In Fatty Liver Disease, Anusha L. Murshed

University of the Pacific Theses and Dissertations

The unfolded protein response (UPR) is composed of three highly conserved pathways (ATF6, IRE1, PERK). Cellular stressors induce protein misfolding and aggregation in the endoplasmic reticulum (ER). This signaling pathway maintains protein homeostasis when there is stress in the ER. When the UPR is activated, the eukaryotic initiation factor 2 alpha (eIF2α) becomes phosphorylated, which inhibits global mRNA translation. If ER stress remains chronically unmitigated, the UPR induces apoptosis. GADD34 and CReP shift in expression when the UPR is activated and work as phosphatases and dephosphorylate eIF2α in a feedback loop, allowing protein synthesis to resume. Several human diseases, including …