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Analysis Of Adc2 And Samdc1 Expression And The Effects Of Transgenic Manipulation Of Polyamine Biosynthesis On Flower Development In Arabidopsis, Vasavi Rani Challa

Master's Theses and Capstones

Polyamines are low molecular weight, aliphatic cationic molecules playing a vital role in all cellular and physiological processes such as cell growth, differentiation, abiotic stress, and somatic embryogenesis. The present study is aimed at unraveling their role during floral development in Arabidopsis thaliana by studying the temporal and spatial expression of ADC2 and SAMDC1 genes by promoter-reporter fusion analysis, and by the transgenic expression of mouse ODC and carrot SAMDC under tissue specific promoters AP1 and AP3, respectively. The expression of SAMDC1 was found to be prominent during different stages of development and in different whorls of flowers whereas ADC2 …

The Development And Utilization Of Est (Expressed Sequence Tag) Resources In The Diploid Strawberry Model System, Robin Leigh Brese

Master's Theses and Capstones

Enhancement of genomic resources is needed for the cultivated octoploid strawberry Fragaria x ananassa (2n=8x=56). A diploid (2n=2x=14) relative, Fragaria vesca, has been used as a model system for strawberry genomics because of its small (~200 Mb) basic genome size, ancestry to the octoploid strawberries and availability of inbred lines, among other favorable features.

In this study, a cDNA library was constructed from developing flower buds of Fragaria vesca ssp. vesca cv. 'Yellow Wonder'. A cDNA library of 7680 clones was created using the CLONTECH SMART cDNA Library Construction kit. Sequencing and bioinformatic analyses of 3298 clones resulted in 2717 …

Microvascular Endothelial Cells Of The Bovine Corpus Luteum: A Comparative Study Of The Estrous Cycle And Pregnancy, Jessica A. Cherry

Master's Theses and Capstones

Endothelial cells derived from the corpus luteum (CLENDOs) exhibit a diverse array of characteristics that seemingly complement their wide-ranging properties in luteal function and fate. Reproductive status of the animal (i.e., non-pregnant vs. pregnant) may contribute to the phenotypic/physiologic diversity of microvascular endothelial cells, but information in this regard is limited. Here, structural and functional attributes of CLENDOs derived from the estrous cycle (Days 9-12) were compared with those of pregnancy (Day 60). Initially, lectin binding properties were examined in the cells. Using fluorescent-tagged lectins for Bandeiraea simplicifolia I or concanavalin A, flow cytometric analysis indicated that lectin-binding on CLENDOs …

Effects Of Stz Induced Hyperglycemia And Hyperlipemia On Ppargamma And Abca1 In A Hamster Model Of Atherogenesis, Craig Lamarca

Master's Theses and Capstones

Diabetes mellitus accelerates atherogenesis, but the exact mechanisms involved are unknown. This study examined the effects of hyperglycemia on the inflammatory transcription factor peroxisome proliferator activated receptor gamma (PPARgamma), known to be involved in atherogenesis, and the ATP binding cassette A1 (ABCA1) protein that is a downstream product of PPARgamma. ABCA1 is a membrane bound transporter that is involved in reverse cholesterol transport via high density lipoprotein cholesterol (HDL-C). Hamsters were divided into two groups; hyperlipemic animals fed a high fat/high cholesterol control diet (C) and hyperlipemic/hyperglycemic animals that received the high fat diet and were administered streptozotocin. Half of …

Studies Of Flower Color In Anagallis Monelli L, Andrea Quintana Pinilla

Master's Theses and Capstones

One of the main goals for plant breeders in the floriculture industry is to develop plants with new attributes. Characteristics such as flower size, growth habit, resistance to pests and diseases, flower size, and particularly flower color are among the most important traits to breed and improve. One of the best ways to make a plant popular in the market is to offer a wide variety of flower colors. A. monelli is an ornamental plant used for hanging baskets and as a bedding plant, and until recently, cultivars available were only blue- or orange-flowered. Recent breeding research on this species …

Temporal Expression Of Protein Mediators During Pgf2alpha-Induced Luteolysis In Sheep, Bryon F. Ricketts

Master's Theses and Capstones

To study dynamic in vivo biochemical changes within the corpus luteum (CL) during luteolysis, a physiological model mimicking the onset of natural luteolysis was established, in which sheep received sequential systemic pulses of PGF2alpha (20ug/min/lhr) at mid-luteal phase of the estrous cycle. We previously demonstrated that after one pulse of PGF2alpha, the protein levels of extracellular matrix (ECM) regulators, tissue inhibitors of metalloproteinases (TIMPs) -1 and -2 decreased dramatically within one hour of PGF2alpha infusion, while matrix metalloproteinase (MMP)-2 activity increased 4 hrs post infusion. In summary, the early and dramatic decrease in TIMP-1 and TIMP-2 proteins, accompanied by an …

Patterns And Processes Of Soil Carbon Dynamics In A Northeastern United States Forest, Sarah K. Silverberg

Master's Theses and Capstones

Forest soils represent a substantial component of the terrestrial carbon cycle and are an important research area for a number of carbon cycle science initiatives. Whereas patterns of aboveground productivity have been relatively well measured and are increasingly included in regional-scale model analyses, belowground estimates are still highly uncertain and progress has been hampered by methodological difficulties. The lack of data poses a problem because belowground measurements are needed to create complete carbon budgets for terrestrial ecosystems at local, regional and global scales. Ecosystem carbon balances will help identify how and where carbon is being stored, as well as how …

Abiotic Retention Of Nitrogen And Dissolved Organic Matter By Forest Mineral Soils, Naoko Watanabe

Master's Theses and Capstones

The effect of NO3- deposition on NH4 +, DON, and DOC retention as well as abiotic NO3 - retention via the hypothesized "ferrous wheel" mechanism was examined in oxic and anoxic tropical and oxic temperate soils using sorption isotherm experiments. Adsorption of NH4+, DON, and DOC by mineral soils was examined in forest floor extracts with DOC levels of 0-50 mg/L. Experimental treatments included no added NO3-, 4mg/L of added NO3-, or 4 mg/L of NO3 - and 2 mg/L of Fe3+. Ferric iron was added since it would likely increase DOC retention in the context of podzolization, and it …

A Test Of The Nutrient-Productivity Model In The Gulf Of Maine Using The Intertidal Mussel Mytilus Edulis, Meredith A. Bailey

Master's Theses and Capstones

Historical concepts of top-down control (predator-prey interactions) on rocky intertidal community structure have transitioned to studies on bottom-up effects (nutrient supply and larval transport) as significant factors affecting rocky intertidal community structure. Studies performed on rocky intertidal locations along the Gulf of Maine (GOM) at multiple sites and seasons in 2004 and 2006 examined the ecology of Mytilus edulis populations by measuring size frequency distributions, diet quality (stable isotope composition) and physiological performance of individuals using condition indices and RNA/DNA ratios. Data were correlated to satellite imagery for sea surface temperature and chlorophyll a concentration and individuals were genetically tested …

Calcium-Dependent Protein Kinases In Physcomitrella Patens, Kathryn Chervincky

Master's Theses and Capstones

Calcium-dependent protein kinases (CDPKs) are a group of plant serine-threonine kinases that are stimulated in response to increases in intracellular calcium concentrations. Physcomitrella patens is a moss and a member of the bryophyte family. Physcomitrella has gained popularity as a model research organism due to the simple morphology of the plant as well as many other desirable characteristics. My research utilized Physcomitrella to accomplish four goals. The first goal was to identify and obtain sequence for as many CDPKs as possible in Physcomitrella. The second goal was to confirm the calcium-activated protein kinase activity of the identified CDPKs. The third …

Correlating Sea Otter Density And Behavior To Habitat Attributes In Prince William Sound, Alaska: A Model For Prediction, Heather A. Coletti

Master's Theses and Capstones

As a benthic foraging marine mammal, sea otters ( Enhydra lutris) present a unique opportunity for conducting a quantitative assessment of behavior based on habitat use as well as developing a habitat based density model using GIS because of the sea otter's well defined habitat requirements. Several studies have documented sea otter behavior but none have calculated the probability of occurrence of a particular behavior based on habitat attributes. Previous predictive models of sea otter density have been constructed, however these models have excluded offshore habitat. Seven aerial surveys, that included offshore habitats, were conducted between 1995 and 2005 in …

Environmental Effects On Early Life Stages Of American Shad (Alosa Sapidissima) And Rainbow Smelt (Osmerus Mordax ), Kristen Fuda

Master's Theses and Capstones

Recruitment of American shad (Alosa sapidissima) and rainbow smelt (Osmerus mordax) has steadily declined over the last few decades, possibly due to the construction of physical impediments to migration and increases in anthropogenic pollution. In order to elucidate environmental parameters influencing early life stages of anadromous fish, both laboratory and field studies were conducted. The effects of abiotic factors, including dissolved oxygen (DO), pH, salinity, nitrate, and phosphate, on hatch and survival of larval and juvenile American shad and rainbow smelt were examined in laboratory studies. Field studies on shad emigration were conducted in the Exeter River, and studies on …

An Experimental Approach To Understanding The Impact Of Vernal Pool Buffer Size On Wood Frogs (Rana Sylvatica), Nicole Alexis Freidenfelds

Master's Theses and Capstones

Strategies to conserve vernal pool-dependent amphibians emphasize the maintenance of adequate areas (buffers) of terrestrial habitat surrounding the pool. I conducted a large-scale field study that specifically examined the effects of buffer width manipulation on adult and metamorph wood frogs at eleven vernal pools in a managed forest in central Maine. Buffer width treatments were 30 m or 100 m forest buffer surrounded by a 100 m clearcut, or no cut (reference). I encircled pools with drift fences and monitored wood frogs from April--November, 2004 and 2005 using pitfall traps and radio-telemetry. Buffer width had no effect on the number …

Characteristics And Dynamics Of A Moose Population In Northern New Hampshire, Anthony Richard Musante

Master's Theses and Capstones

Abundance indices suggest that the moose (Alces alces) population in northern New Hampshire has stabilized despite favorable habitat and conservative harvest. Natural mortality of unknown cause is presumed a primary reason although little is known about moose reproduction and survival in New Hampshire. This study (2002-2005) was designed to investigate the dynamics that impact this population.

Analysis of harvest reproductive data (1988-2004) indicated that average field-dressed weight of adult cows increased although corpora lutea count declined from ∼1.4- 1.2/cow in the study area and statewide. Yearling ovulation rate (∼42%) and average weight (<211 kg) declined about 25% and 4%, respectively. Parturition of radio-marked cows ranged from 8 May-13 July (median=19 May) with 78% of births from 13-27 May. Calving rate of yearlings and adults (>2 yr) averaged 30 and 85%, respectively, …

Identifying Exclusion Genes Of Bacteriophage Hk239, Ali Wright

Mahurin Honors College Capstone Experience/Thesis Projects

Temperate bacteriophage can adopt either a lytic or a lysogenic lifestyle. In the lytic lifestyle, the bacteriophage infects a cell, uses the host's cellular machinery to replicate, and lyses the cell to release the phage progeny. In the lysogenic lifestyle the bacteriophage genome is integrated into the bacterial genome to generate a prophage. Most of the genes of the prophage are repressed. However, those genes that are expressed have a wide variety of functions including exclusion, the prevention of phage infection. Lysogens of bacteriophage HK239 have the ability to exclude a wide variety of phage, including (lambda), T4rII, Plvir, P2, …

Interactive 3d Multimedia Learning Tools In Biology / Chin Siaw Khim, Siaw Khim Chin

Student Works (2000-2009)

An interactive 3D Multimedia Learning tool in Biology is an application system to assist users in the brand new learning style for biology. Combination of the virtual and multimedia in the system can bring out the clear explanation of processes and delivery the information. Besides that, the system enables users to interactive with the system to help them in the learning biology. The web-based application also encourage sharing knowledge through distance learning between the user. An interactive 3D Multimedia Learning tools in Biology mainly focus on Biology Cell topic with SPM syllabus. The target user for this system is mainly …

Establishment Of Polarity During Organization Of The Acentrosomal Plant Cortical Microtubule Array, Ram Dixit, Eric Chang, Richard Cyr

Biology Faculty Research

The plant cortical microtubule array is a unique acentrosomal array that is essential for plant morphogenesis. To understand how this array is organized, we exploited the microtubule (+)-end tracking activity of two Arabidopsis EB1 proteins in combination with FRAP (fluorescence recovery after photobleaching) experiments of GFP-tubulin to examine the relationship between cortical microtubule array organization and polarity. Significantly, our observations show that the majority of cortical microtubules in ordered arrays, within a particular cell, face the same direction in both Arabidopsis plants and cultured tobacco cells. We determined that this polar microtubule coalignment is at least partially due to a …

Toxin-Producing Microcystis Aeruginosa: A Trade-Off In The Vertical Distribution Of Three Daphnia Species As Predicted By The Ideal Free Distribution With Costs Model, Sonya Claudine Carlson

Master's Theses and Capstones

Daphnia hyalina, D. galeata, and D. ambigua were exposed to the cyanobacterium Microcystis aeruginosa in 30 cm temperature-stratified columns. Changes in egg ratio, lipid index, length, and growth were recorded over 3 day experiments. Spatial distributions were recorded during 6-hour constant light experiments and 3 day fluctuating light experiments. D. hyalina and D. galeata stayed in the upper half of the column in temperatures of 18--22°C when exposed to M. aeruginosa. D. ambigua remained between 10--19°C regardless of food type. For across all experiments, depth and temperature ranges were narrowest in treatments with M. aeruginosa. D. ambigua incurred costs to …

Effects Of Forest Clear Cutting On Spotted Salamander ( Ambystoma Maculatum) Migration, Jessica Susannah Veysey

Master's Theses and Capstones

Upland buffer zones are a proposed management tool for vernal-pool-breeding amphibians. Substantial validation of buffers, via experimental upland habitat disturbance, is lacking. Specifically, no studies have examined immediate effects of clear cutting on spotted salamander (Ambystoma maculatum ) migration. I used clear cutting to experimentally manipulate upland buffer widths at 11 vernal pools. I then radiotracked 40 adult spotted salamanders at these pools, and modeled their migration with mixed-effects regression. Mean maximum distance from the pool was 106.0 +/- 15.4 m (range = 1.6 to 427.6 m). At clear cut-treatment pools, mean percent of time in the cut was 27.2 …

Mhc (B) Complex Recombinant Immune Response, Nicole Gay Wilkinson

Master's Theses and Capstones

Recombination between chicken major histocompatibility complex (MHC) class I B-F and class IV B-G genes has enabled more precise identification of genes controlling immune responses. Congenic lines, each containing a unique MHC recombinant, were developed with 99.9% genetic uniformity to inbred Line UCD 003 (B17B17). A new recombinant, R13 (BF17-BG23), originated in the tenth backcross generation for R1 (BF24-BG23). Two experiments tested the R13 immune response to bovine red blood .cells (BRBC) and Rous sarcoma virus (RSV).

A single R13B17 sire mated to five R131317 dams produced progeny segregating for R13R13, R13B17, and B17B17 genotypes. These progeny were injected with …

Salmonella Enterica Serovar Typhimurium Promotes Apoptosis In Activated Neutrophils, Christopher C. Wrocklage

Master's Theses and Capstones

Apoptosis is an extremely important and highly regulated function in neutrophils. As the most abundant, but shortest-lived white blood cells, they must initiate this process in the absence of pathogens and selectively release their potent cytotoxic components when pathogens are present. Many pathogens are able to modulate this process in neutrophils, either for the host's benefit or for their own. Previous work has shown that transmigration across a T84 intestinal epithelial crypt cell monolayer increases the ability of neutrophils to bind and kill Salmonella enterica serovar Typhimurium (S. Typhimurium).

The goal of this work was to see how S. Typhimurium …

Roles Of Wrky Proteins In Mediating The Crosstalk Of Hormone Signaling Pathways: An Approach Integrating Bioinformatics And Experimental Biology, Zhen Xie

UNLV Retrospective Theses & Dissertations

The goal of my research is to understand the molecular mechanism by which hormones control seed germination. Gibberellins (GA) promote, while abscisic acid (ABA) and salicylic acid (SA) inhibit seed germination. Key molecules in these signaling pathways include receptors, secondary messengers, protein kinases and phosphatases, and transcription factors. My study focuses on how WRKY transcription factors modulate the expression of an alpha-amylase gene (Amy32b), which is up-regulated by GA, but down-regulated by ABA and SA in the aleurone cells of germinating seeds; Chapter 2 started with the annotation and phylogenetic analyses of the WRKY gene superfamily, followed by functional studies …

Transposable Elements: What Have You Done For Me Lately? A Genomics Based Investigation Into The Potential Functional Roles Of Transposable Elements Using The Model Organism Caenorhabditis Elegans, Sarah Prescott Kenick

Doctoral Dissertations

The genomes of all organisms contain discrete DNA sequences present as disperse repetitive elements called transposons. Transposons have the unique ability to move to new chromosomal locations. Problems of uncontrolled movement of transposons can result in mutations, rearrangement, and even broken chromosomes. Often termed "selfish parasites" that invade a host genome, there is a longstanding question of whether they have a functional role. As a first step in an effort to investigate this question, I identified and annotated 276 full length and partial elements in the C.elegans genome. I determined the genomic location of each and looked for patterns resulting …

Activation Of Toll-Like Receptor 2 Through Tlr2-Lrr Binding Synthetic Peptides, Christopher Benton

Master's Theses and Capstones

Bacterial sepsis and systemic inflammatory immune responses continue to be major causes of illness and death despite extensive research into the development of antimicrobial agents. There is a need for novel therapeutic reagents designed to modulate these responses. Toll-like receptor 2 plays a key role in the development of innate and adaptive immune responses to microbial products that interact with the receptor's extracellular leucine-rich repeat ligand-binding domain. In a preliminary study, five peptides were synthesized that bound to the leucine-rich repeat region of Toll-like receptor 2 (TLR2). We tested the hypothesis that the novel TLR 2 leucine rich repeat binding …

Behavioral Genetics And Crime, In Context, Owen D. Jones

Vanderbilt Law School Faculty Publications

This Article provides an introduction to some of the key issues at the intersection of behavioral genetics and crime.

It provides, among other things, an overview of the emerging points of consensus, scientifically, on what behavioral genetics can and cannot tell us about criminal behavior. It also discusses a variety of important implications (as well as complexities) of attempting to use insights of behavioral genetics in legal contexts.

Contribution To The Taxonomical And Biological Characteristics Of Sciurus Anomalus In Turkey (Mammalia: Rodentia), İrfan Albayrak, Ati̇lla Arslan

Turkish Journal of Zoology

This study is based on 62 Sciurus anomalus specimens obtained from Konya, Karaman, Kütahya and Çorum provinces between 1997 and 2002. Some ecological and biological features of the species, including habitat, nest-sites, fur colour, baculum and phallus were investigated. In this species, the chromosome number (2n) is 40. For the taxonomical evaluation of Sciurus anomalus, morphometric data of the specimens were used. The specimens were discussed at the subspecies level, comparing to the relevant literature, and it was concluded that our specimens represent the nominate form.

An Analysis Of Tennessee Gateway Exams And The Variables Related To Student Results., Judy W. Webb

Electronic Theses and Dissertations

The purpose of this study was to examine the variables that are associated with the percentage of students who pass Tennessee's Gateway exams in high school. Associations were examined between the Gateway exams and variables such as: socioeconomic status, ethnicity, dropout rate, graduation rate, attendance, average daily membership, per-pupil expenditure, teachers' salary, and elementary-school reading scores.

Pearson correlations between school characteristics and pass rates of three Gateway exams were calculated. Schools were divided into quartiles based upon socioeconomic status and ethnicity in order to examine pass rates of the Gateway exams among different quartiles. Independent samples t tests were performed …

Fabrication Of A Microfluidic Platform For Impedance Analysis Of Cultured Endothelial Cell Monolayers., Stuart J. Williams 1981-

Electronic Theses and Dissertations

Assessing the functionality of the endothelium can provide insight into the initiation and formation of arterial diseases. One of the most important functions of the endothelial layer is its permeability. The integrity of the cell monolayer and its ability to transport molecules can be assessed in vitro by investigating the electrical impedance. In this study, a microfluidic platform was created using an electrode-patterned glass substrate and microfluidic poly(dimethyl siloxane) (PDMS) substrate. The electrode glass base of the structure was fabricated with platinum square electrodes of various sizes ranging from 10x10 ìm2 to 160x160 ìm2 and a larger, common counter electrode. …

Photochemical Transformations Of Riverine Dissolved Organic Matter: Effects On Estuarine Bacterial Metabolism And Nutrient Demand, Erik M. Smith, Ronald Benner

Faculty Publications

By experimentally simulating the exposure of terrigenous dissolved organic matter (DOM) to sunlight during the mixing of river water and seawater in an estuary (St. Helena Sound, South Carolina), this study examined how photochemical alterations may affect both the magnitude and fate of DOM utilization by estuarine bacteria. In a series of experiments performed with water samples collected from 3 blackwater rivers draining into an estuary of the South Atlantic Bight (USA), exposure to light resulted in significant decreases in dissolved organic carbon (DOC) concentration and DOM absorption at 350 nm relative to dark controls. When these water samples were …

The Obligation To Endure Again, Sandra Steingraber

Taproot: A Journal of Outdoor Education

The author of this letter was a young biologist named Rachel Carson. Carson could not have known, when she penned it, how profound an impact her short letter would have. The Associated Press picked it up for syndication. The Reader's Digest reprinted it. And so her missive found its way onto front porches and breakfast tables, into living rooms and libraries and doctors' office waiting rooms across the nation. At the height of the Cold War, in the midst of an anti-Communist inquisition that had convulsed the federal government, Carson's assertion that "the real wealth of the Nat ion lies …