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Diagnosis And Prevention Of Metabolic Diseases In Drosophila Melanogaster, Claire Marie Coogan

Collection of Engaged Learning

No abstract provided.

Plant-Pollinator Interactions Over 120 Years: Loss Of Species, Co-Occurrence, And Function, Laura A. Burkle, John C. Marlin, Tiffany M. Knight

Biology Faculty Research

Using historic data sets, we quantified the degree to which global change over 120 years disrupted plant-pollinator interactions in a temperate forest understory community in Illinois, USA. We found degradation of interaction network structure and function and extirpation of 50% of bee species. Network changes can be attributed to shifts in forb and bee phenologies resulting in temporal mismatches, nonrandom species extinctions, and loss of spatial co-occurrences between extant species in modified landscapes. Quantity and quality of pollination services have declined through time. The historic network showed flexibility in response to disturbance; however, our data suggest that networks will be …

Liberal Knowledge, St. Norbert College

St. Norbert College Magazine 2013-2018

In a scientific society transformed by the internet, the liberal arts are essential in forming effective, responsible scientists. Michael Foley ’84, chemical biology platform director for the Broad Institute of MIT and Harvard University, explains why.

Persons As Plants: Ecopsychology And The Return To The Dream Of Nature, Monica Gagliano

Landscapes: the Journal of the International Centre for Landscape and Language

Abstract. In this article, I examine human-plant perceptions and interactions in terms of developing a new perspective on the perception and the actions of people towards plants. By combining my scientific understanding of the biological world and my own experiences working with plant shamans, storytellers and mystics from around the world, I engage with the idea that the hierarchical structure by which Western science defines the variety of life forms as ‘primitive’ or ‘more evolved’ is at the root of the current environmental crisis and I argue that the solution to it rests in a change of this very perspective.

Under Graduate Curriculum Integration: Bringing Students Together Through Research, Clay Runck, James Russell, Allison D’Costa

Interdisciplinary STEM Teaching & Learning Conference (2012-2019) (Archived)

At Georgia Gwinnett College we are integrating the undergraduate educational experience for our Bachelor of Science students in the Biology discipline. We are using the opportunities presented by the genetic information revolution to combine biology courses in a longitudinal and integrative research project aimed at cataloguing biological diversity over space and time on the developing Georgia Gwinnett College campus.

Integrating Art And Science In Undergraduate Education, Daniel Gurnon

Chemistry & Biochemistry Faculty Publications

The prevailing vision for undergraduate science education includes increased collaboration among teachers of science, technology, engineering and math (STEM) and an overhaul of introductory courses [1]–[4]. But by staying within the borders of STEM, are we overlooking connections between the arts and innovative science? Likewise, are we missing an important opportunity to inspire and inform nonscientists? Here we explore how weaving the visual arts into a science curriculum can both help develop scientific imagination and engage nonscientists. As an example, we describe a recent collaboration between artists and scientists to create a series of science-inspired sculptures.

Diamond V. Chakrabarty: Oil Eaters: Alive And Patentable, Dennis J. Walsh

Pepperdine Law Review

Congress is empowered, under article I, section 8 of the United States Constitution, to create patent laws that encourage the promotion of arts and sciences. In the congressional fulfillment of this task, the courts have been confused as to what products are worthy of patent protection under the patent statutes. One illustration of this confusion is the recent controversy of whether living organisms fit into the statutory patentable classification of section 101 of the 1952 Patent Act. The recent United States Supreme Court decision of Diamond v. Chakrabarty has ended this confusion by holding that living micro bacteria is patentable …

S-Adenosyl-L-Methionine Protects The Probiotic Yest, Saccharomyces Boulardii, From Acid-Induced Cell Death, Nicanor Austriaco, Vincent Cascio, Daniel Gittings, Kristen Merloni, David Laprade

Biology Faculty Publications

Background

Saccharomyces boulardii is a probiotic yeast routinely used to prevent and to treat gastrointestinal disorders, including the antibiotic-associated diarrhea caused by Clostridium difficile infections. However, only 1-3% of the yeast administered orally is recovered alive in the feces suggesting that this yeast is unable to survive the acidic environment of the gastrointestinal tract.

Results

We provide evidence that suggests that S. boulardii undergoes programmed cell death (PCD) in acidic environments, which is accompanied by the generation of reactive oxygen species and the appearance of caspase-like activity. To better understand the mechanism of cell death at the molecular level, we …

Multiple Breast Cancer Cell-Lines Derived From A Single Tumor Differ In Their Molecular Characteristics And Tumorigenic Potential, Goar Mosoyan, Chandandeep Nagi, Svetlana Marukian, Avelino Teixeira, Anait Simonian, Lois Resnick-Silverman, Analisa Difeo, Dean Johnston, Sandra R. Reynolds, Daniel F. Roses, Arevik Mosoian

Publications and Research

Background Breast cancer cell lines are widely used tools to investigate breast cancer biology and to develop new therapies. Breast cancer tissue contains molecularly heterogeneous cell populations. Thus, it is important to understand which cell lines best represent the primary tumor and have similarly diverse phenotype. Here, we describe the development of five breast cancer cell lines from a single patient’s breast cancer tissue. We characterize the molecular profiles, tumorigenicity and metastatic ability in vivo of all five cell lines and compare their responsiveness to 4-hydroxytamoxifen (4-OHT) treatment. Methods Five breast cancer cell lines were derived from a single patient’s …

Integration Of Nodal And Bmp Signals In The Heart Requires Foxh1 To Create Left–Right Differences In Cell Migration Rates That Direct Cardiac Asymmetry, Kari F. Lenhart, Nathalia G. Holtzman, Jessica R. Williams, Rebecca D. Burdine

Publications and Research

Failure to properly establish the left–right (L/R) axis is a major cause of congenital heart defects in humans, but how L/R patterning of the embryo leads to asymmetric cardiac morphogenesis is still unclear. We find that asymmetric Nodal signaling on the left and Bmp signaling act in parallel to establish zebrafish cardiac laterality by modulating cell migration velocities across the L/R axis. Moreover, we demonstrate that Nodal plays the crucial role in generating asymmetry in the heart and that Bmp signaling via Bmp4 is dispensable in the presence of asymmetric Nodal signaling. In addition, we identify a previously unappreciated role …

Dictyostelium Development Shows A Novel Pattern Of Evolutionary Conservation, Xiangjun Tian, Joan E. Strassmann, David C. Queller

Biology Faculty Research

von Baer's law states that early stages of animal development are the most conserved. More recent evidence supports a modified "hourglass" pattern in which an early but somewhat later stage is most conserved. Both patterns have been explained by the relative complexity of either temporal or spatial interactions; the greatest conservation and lowest evolvability occur at the time of the most complex interactions, because these cause larger effects that are harder for selection to alter. This general kind of explanation might apply universally across independent multicellular systems, as supported by the recent finding of the hourglass pattern in plants. We …

Functional Neural Correlates Of Attentional Deficits In Amnestic Mild Cognitive Impairment, Nicholas T. Van Dam, Mary Sano, Effie M. Mitsis, Hillel T. Grossman, Xiaosi Gu, Yunsoo Park, Patrick R. Hof, Jin Fan

Publications and Research

Although amnestic mild cognitive impairment (aMCI; often considered a prodromal phase of Alzheimer’s disease, AD) is most recognized by its implications for decline in memory function, research suggests that deficits in attention are present early in aMCI and may be predictive of progression to AD. The present study used functional magnetic resonance imaging to examine differences in the brain during the attention network test between 8 individuals with aMCI and 8 neurologically healthy, demographically matched controls. While there were no significant behavioral differences between groups for the alerting and orienting functions, patients with aMCI showed more activity in neural regions …

High Levels Of Adherence And Viral Suppression In A Nationally Representative Sample Of Hiv-Infected Adults On Antiretroviral Therapy For 6, 12 And 18 Months In Rwanda, Batya Elul, Paulin Basinga, Harriet Nuwagaba-Biribonwoha, Suzue Saito, Deborah Horowitz, Denis Nash, Jules Mugabo, Veronicah Mugisha, Etienne Rugigana, Richard Nkunda, Anita Asiimwe

Publications and Research

Background Generalizable data are needed on the magnitude and determinants of adherence and virological suppression among patients on antiretroviral therapy (ART) in Africa. Methods We conducted a cross-sectional survey with chart abstraction, patient interviews and site assessments in a nationally representative sample of adults on ART for 6, 12 and 18 months at 20 sites in Rwanda. Adherence was assessed using 3- and 30-day patient recall. A systematically selected sub-sample had viral load (VL) measurements. Multivariable logistic regression examined predictors of non-perfect (<100%) 30-day adherence and detectable VL (>40 copies/ml). Results Overall, 1,417 adults were interviewed and 837 had VL measures. Ninety-four percent and 78% …

The Telomere Capping Complex Cst Has An Unusual Stoichiometry, Makes Multipartite Interaction With G-Tails, And Unfolds Higher-Order G-Tail Structures, Neal F. Lue, Ruobo Zhou, Lidia Chico, Ninghui Mao, Olga Steinberg-Neifach, Taekjip Ha

Publications and Research

The telomere-ending binding protein complex CST (Cdc13-Stn1-Ten1) mediates critical functions in both telomere protection and replication. We devised a co-expression and affinity purification strategy for isolating large quantities of the complete Candida glabrata CST complex. The complex was found to exhibit a 2∶4∶2 or 2∶6∶2 stoichiometry as judged by the ratio of the subunits and the native size of the complex. Stn1, but not Ten1 alone, can directly and stably interact with Cdc13. In gel mobility shift assays, both Cdc13 and CST manifested high-affinity and sequence-specific binding to the cognate telomeric repeats. Single molecule FRET-based analysis indicates that Cdc13 and …

Regulation Of Antigenic Variation In Trypanosoma Brucei, Imaan A. Benmerzouga

ETD Archive

Trypanosoma brucei is a protozoan parasite that causes sleeping sickness in humans and nagana in cattle. When inside the mammalian host, T. brucei cells stay in extracellular spaces and regularly switch their surface antigen, Variant Surface Glycoprotein (VSG), to escape the host's immune responses. To ensure the effectiveness of VSG switching, T. brucei expresses a single type of VSG at any time exclusively from one of 20 identical VSG expression sites located next to the telomere. Monoallelic expression of VSG and VSG switching are important for T. brucei's pathogenesis. Our major goal is to understand the mechanisms of antigenic variation …

Interpreting Biological Similarity: Ongoing Challenges For Diverse Decision Makers, Sarah M. Cork

Michigan Telecommunications & Technology Law Review

Similarity is an elusive and complicated concept facing comparisons of biological molecules, as even minute changes to a molecule's structure can dramatically affect its function in the body. Yet the flood of biologic drugs on the market will increasingly force these similarity comparisons. These concerns are particularly relevant to two groups of drugs: families of biologic drugs that closely resemble each other in structure and function, here termed "similar-impact biologics," and the biosimilars, which are intended to closely approximate generic forms of biologic drugs. In bringing biologic drugs to the market, manufacturers are likely to face dual obstacles: FDA approval …

Difference In Water Status Between Oak Trees (Quercus Berberidifolia) With Brown And Green Leaves During Severe Drought, Mia Bryant, Hannah Dario, Cameron Kline

Featured Research

In this experiment, we examined cavitation in Quercus berberidifolia plants. We hypothesized that as the amount of water stress increases, then the pressure to cause embolism will decrease. We thought this because as a plant loses more water, the more air bubbles will form within the plant and therefore the pressure to cause this will decrease. We tested this hypothesis by comparing dry Quercus berberidifolia to more hydrated Quercus berberidifolia in Tapia Park, near Malibu, California. We measured and compared these plants by the use of the Scholander Pressure chamber and a parometer. In the end, we discovered that our …

Varying Carbon Dioxide Levels And Its Effects On Malsoma Laurina’S Photosynthetic Rate, John A. Lawlor, Dustyn T. Zierman-Felix, Michael P. Schlagel

Featured Research

The Keeling Curve has displayed an exponential increase in carbon dioxide within the earth’s atmosphere since the late 1950’s. Scientists have heatedly debated the effects that will occur as a result of this relatively new phenomenon (since the Industrial Revolution of America). We tested the effect of increased carbon dioxide levels on plant life, Malosma laurina, in particular. Our experiment involved the application of 400 PPM of carbon dioxide into different specimens of M. laurina and 800 PPM of carbon dioxide into the same specimens. We then recorded the rate of photosynthesis, conductance results, and levels of internal carbon dioxide. …

A Comparison Of Water Availability In Coastal Live Oak (Quercus Agrifolia) And California Bay (Umbellularia Californica) At Top And Bottom Of Hillsides At Malibu Creek State Park, California, Jake Szarzynski, Marley Biyendolo, Jeremy Goldenetz, Ann Alcazar

Featured Research

This investigation serves to show whether moisture content is different in plants at the top of hills versus bottom of hills, specifically comparing values of Quercus agrifolia and Umbellularia californica in Malibu Creek State Park. By acquiring six branches of each species on a hill at intervals of twenty feet, the moisture content can be obtained and analyzed by using the Scholander Hammel pressure chamber. This allows us to determine whether there is a correlation between moisture content and plant position on hills.

Impact Of Reclaimed Water On Photosynthetic Performance In Green Bark Ceanothus (Ceanothus Spinosus), Erika De Leener, Michael L. Gribble, Joshua D. Volkmar, Luo J. Zhao

Featured Research

The sprinkled water on the campus of Pepperdine University is primarily reclaimed water from sinks, toilets and showers on campus. The water is treated with micro bacteria and sand rocks to remove larger particles, smaller nitrogen containing compounds, excess nutrients and human pathogens. The Mediterranean weather in Southern California is characterized by dry summers, and watering plants by reclaimed water helps relieve the water stress in the community. However, the reclaimed water might distort the normal physiology of native plants on campus. We hypothesize that because nitrogen containing compounds cannot be efficiently removed by micro bacteria, higher nitrogen content in …

The Rho-Gef Akap-Brx Is A Co-Activator Of Progesterone Receptor B In Uterine Leiomyoma Cells, Soledad Jorge

Yale Medicine Thesis Digital Library

Background--Uterine leiomyomas, or fibroids, are prevalent tumors that are dependent on sex steroids for growth. Clinically, progesterone promotes fibroid growth and anti-progestins cause a durable reduction in fibroid size, yet the mechanisms underlying progesterone action are unclear. A-kinase anchoring protein 13 (AKAP-13, also known as AKAP-Brx) functions as a Rho-GEF and is over-expressed in fibroids. AKAP-Brx has been shown to activate estrogen and glucocorticoid receptors, and bind these receptors via a nuclear receptor-binding motif. However, it is not known whether AKAP-Brx also binds progesterone receptor or augments progesterone action.

Objective--The purpose of this study was to determine if AKAP-Brx affected …

Lifelines Winter 2013, Southern Adventist University

Lifelines - Biology Department Newsletter

The Winter 2013 issue of Lifelines features updates on research being conducted in the Biology Department, updates to the Biology Trail, student missions to Familia Feliz in Bolivia, and a pictorial directory of the 2013 biology and allied health graduates.

Singing Behavior And Geographic Variation In The Songs Of The Veery (Catharus Fuscescens) Across The Appalachian Mountains, Courtney L. Brennan

ETD Archive

The Veery (Catharus fuscescens) is a common Neotropical migrant thrush whose breeding distribution spans the Northern United States and southern Canada and continues south through the high elevations of the Appalachian Mountains. Despite its wide distribution, many aspects of Veery biology are largely unknown, including a clear characterization of song and knowledge of geographic song variation. I describe the song structure, repertoire size, and patterns of song delivery in the songs of the Veery and describe geographic song variation across four regions along the Appalachian Mountains. Visual analysis of song spectrogram images of territorial males revealed that Veeries' repertoire ranges …

Bioinformatic Analysis And In Vitro Expression Of Malaria Parasite Translocon And Ribonuclease Binding-Like Rhoptry Genes, Moses Z. Timta

ETD Archive

Malaria caused by the parasite Plasmodium, still remains a significant public health problem worldwide, due to lack of a vaccine and emerging drug and insecticide resistance, among malaria parasites and mosquito vectors, respectively. Rhoptry proteins of Plasmodium enable merozoite invasion of host erythrocytes. However, only a few of these proteins have been characterized. Thirty-six P. yoelii merozoite rhoptry proteins were identified as putative rhoptry proteins by proteome analysis. Some of these proteins have been characterized while others still remain an intense area of active research. Molecular characterization and understanding of these novel proteins may assist in vaccine development, design of …

The Evolution Of Ungulate Locomotion Utilizing Extant Taxa For Reconstrucing The Movement Of Extinct Forms, Beth Carroll

Theses & ETDs

Locomotor behavior is a complex neural and musculoskeletal response to an animal's environment and other external factors. Morphology, behavior, and gait choice are closely interrelated and often coevolve in response to environmental change. Morphological, specifically osteological, correlates of specific behaviors and movement patterns observed in extant taxa provides insight into that of extinct groups in which fossilized bones and trackways provide the only clues. Extant taxa can be useful models for reconstructing the movements and behaviors of extinct forms. The mammalian ungulate clades seen today are a representation of a rich and diverse evolutionary history filled with multiple examples of …

Post-Fire Recovery Of Juglans Californica In Sycamore Canyon: Water Potential And Site Characterization, Ariel Lan, Caitlin Thompson, Victoria Lekson

Featured Research

Our study focused on post-fire recovery in the California Black Walnut, Juglans californica. Our study was conducted Sycamore Canyon, an area of the Santa Monica Mountain Range recently damaged by the April 2013 fires. Part of our study focused on water potential and water stress among J. californica; we examined differences in the water potentials of mature, unburned trees and resprouts which have grown since the fires in April. We found that mature trees have a significantly higher water potential than sprouts, either epicormic or basal, which indicates that they are better equipped to handle water stress. We also found …

Post-Fire Recovery Of Juglans Californica In Sycamore Canyon: Vessel Length, Shading, Water Use Efficiency And Water Status, Victoria Lekson, Anushree Mahajan

Featured Research

In April 2013, Sycamore Canyon experienced a major wildfire. This study investigates post-fire recovery of two types of resprouts, basal and epicormic, and compares multiple parameters to mature unburned Juglans californica. In this study, we investigate the influence of time of day on the post-fire water potential of mature unburned plants versus burned Juglans californica epicormic and basal resprouts. One way ANOVA test reveals p

The Effect Of Freezing Conditions On Xylem Diameter Of Malosma Laurina And Umbellularia Californica In The Santa Monica Mountains, Nicole A.P.M.K.O.M. Nakamatsu, Theadora V. Ordog, Kaitlyn E. Sauer

Featured Research

Temperature variations in the Santa Monica Mountains are drastic at times, which may be affecting the local plants. To determine the repercussions of these variations in temperature, we measured the xylem diameters of freezing and non-freezing Malosma laurina and Umbellularia californica. U. californica was chosen because it has a large xylem diameter, yet is known to survive freezing conditions. We thought that this plant could provide the most significant results to prove or disprove our hypothesis. M. laurina was chosen because it is abundant in the Santa Monica Mountains and has different physical dimensions than U. californica. Smaller xylem diameters …

Comparison Of Th1 Cytokines And T Cell Markers Gene Expressions Between Virulent And An Attenuated Eiav Vaccine Strain, Talia R. Henkle

Lewis Honors College Capstone Collection

The equine infectious anemia virus (EIAV) is closely related to HIV and has been used as a model to identify protective mechanisms against lentivirus infection. In horses, EIA infection progresses for about a year before infected horses manage to control virus replication. This naturally-gained protection is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. As the resolution of initial viremia correlates with the appearance of virus specific cytotoxic T lymphocytes (CTL), we believe that cellular immune responses play a key role in controlling EIAV in the horse. In …

Intermittent Swim Stress Effects On Anxiety Behavior, Timothy A. Warner

Doctoral Dissertations

Millions of Americans are suffering from depression each year, leading to a significant number of individuals who seek treatment for their ailment. However, fewer than 50 percent of depressed individuals fully recover using current methods. The comorbidity between depression and anxiety could be a contributing factor in the lower rates of recovery. The demonstrated correlation between anxiety and depression has led to the term "anxious depression," which is associated with difficulty in coping, a poorer rate of recovery, and more severe symptoms of depression. The purpose of this dissertation was to expand on an existing animal model of depression (intermittent …