Digital Commons Network^™

Polydnaviral Ankyrin Proteins Aid Parasitic Wasp Survival By Coordinate And Selective Inhibition Of Hematopoietic And Immune Nf-Kappa B Signaling In Insect Hosts, Gwenaelle Gueguen, Marta E. Kalamarz, Johnny Ramroop, Jeffrey Uribe, Shubha Govind

Publications and Research

Polydnaviruses are mutualists of their parasitoid wasps and express genes in immune cells of their Lepidopteran hosts. Polydnaviral genomes carry multiple copies of viral ankyrins or vankyrins. Vankyrin proteins are homologous to IκB proteins, but lack sequences for regulated degradation. We tested if Ichnoviral Vankyrins differentially impede Toll-NF-κB-dependent hematopoietic and immune signaling in a heterologous in vivo Drosophila, system. We first show that hematopoiesis and the cellular encapsulation response against parasitoid wasps are tightly-linked via NF-κB signaling. The niche, which neighbors the larval hematopoietic progenitors, responds to parasite infection. Drosophila NF-κB proteins are expressed in the niche, and non cell-autonomously …

Mscs-Like Mechanosensitive Channels In Plants And Microbes, Margaret E. Wilson, Grigory Maksaev, Elizabeth S. Haswell

Biology Faculty Research

The challenge of osmotic stress is something all living organisms must face as a result of environmental dynamics. Over the past three decades, innovative research and cooperation across disciplines have irrefutably established that cells utilize mechanically gated ion channels to release osmolytes and prevent cell lysis during hypoosmotic stress. Early electrophysiological analysis of the inner membrane of Escherichia coli identified the presence of three distinct mechanosensitive activities. The subsequent discoveries of the genes responsible for two of these activities, the mechanosensitive channels of large (MscL) and small (MscS) conductance, led to the identification of two diverse families of mechanosensitive channels. …

Β-Catenin-Independent Activation Of Tcf1/Lef1 In Human Hematopoietic Tumor Cells Through Interaction With Atf2 Transcription Factors, Luca Grumolato, Guizhong Liu, Tomomi Haremaki, Sathish Kumar Mungamuri, Phyllus Mong, Gal Akiri, Pablo Lopez-Bergami, Adriana Arita, Youssef Anouar, Marek Mlodzik, Ze'ev A. Ronai, Joshua Brody, Daniel C. Weinstein, Stuart A. Aaronson

Publications and Research

The role of Wnt signaling in embryonic development and stem cell maintenance is well established and aberrations leading to the constitutive up-regulation of this pathway are frequent in several types of human cancers. Upon ligand-mediated activation, Wnt receptors promote the stabilization of β-catenin, which translocates to the nucleus and binds to the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcription factors to regulate the expression of Wnt target genes. When not bound to β-catenin, the TCF/LEF proteins are believed to act as transcriptional repressors. Using a specific lentiviral reporter, we identified hematopoietic tumor cells displaying constitutive TCF/LEF transcriptional activation in …

Factor H Cleavage By The Treponema Denticola Protease Dentilisin: Understanding The Pathogenesis Of Periodontal Disease, Sahil Aggarwal

Undergraduate Research Posters

Periodontal disease is an infectious condition that results in the inflammation and gradual degradation of gum tissue and alveolar bone. Caused by plaque deposits on teeth that harden into tartar to inflame gum tissue, the condition is highly pervasive, with 15 percent of US adults over 30 years of age exhibiting destructive periodontal disease. The abundance of Treponema denticola, a spirochete, in the oral flora is strongly correlated with disease severity. T. denticola binds to Factor H (FH), a negative regulator of the complement system, through its Factor H binding protein (FhbB) to evade complement-mediated killing. The protease dentilisin, produced …

Jamie Jensen Research Interests, Jamie Jensen

BYU Research Development Office Research Networking Conference

Focus of Study: The development and transferability of scientific reasoning skills; appropriate assessment techniques; effective strategies for constructivist teaching in the STEM classroom; and, strategies to enhance STEM retention.

Characterization Of Genes Involved In Sumoylation During Embryogenesis In Rainbow Trout (Oncorhynchus Mykiss), Xiaowei Zang

Graduate Theses, Dissertations, and Problem Reports (ETD)

SUMOylation is the post-translational modification of proteins by the addition of the small ubiquitin-like modifier (SUMO), which plays an important role in various cellular processes. It has been reported that SUMO and its related proteins are important in diverse reproductive functions such as ovulation, gametogenesis, and embryogenesis. Modification of target proteins by SUMO is an ATP-dependent enzymatic cascade involving three key enzymes: E1 activating enzyme (the heterodimer SAE1-SAE2), E2 conjugating enzyme (UBC9) and several E3 ligating enzymes (PIAS, RanBP2/Nup358 and Pc2). The objectives of this study were to characterize the genes involved in SUMOylation and determine their expression profiles during …

A Force Of Nature: Molecular Mechanisms Of Mechanoperception In Plants, Gabriele B. Monshausen, Elizabeth S. Haswell

Biology Faculty Research

The ability to sense and respond to a wide variety of mechanical stimuli-gravity, touch, osmotic pressure, or the resistance of the cell wall-is a critical feature of every plant cell, whether or not it is specialized for mechanotransduction. Mechanoperceptive events are an essential part of plant life, required for normal growth and development at the cell, tissue, and whole-plant level and for the proper response to an array of biotic and abiotic stresses. One current challenge for plant mechanobiologists is to link these physiological responses to specific mechanoreceptors and signal transduction pathways. Here, we describe recent progress in the identification …

Strategies To Improve Oral Health Systems In Rural Vietnam, Dylan Vu

Undergraduate Research Posters

Oral health care systems seek to prevent and treat problems related to the mouth as well as restore teeth and the tissues that support the teeth. Oral health is important because it is closely related to general health and affects quality of life, food consumption, and physical appearance (Uetani et al., 2006). Poor, rural areas of the world especially do not have the oral health care system to keep up with the worsening oral health statuses of their people due to a lack of resources and/or infrastructure (Kandelman et al., 2012). Such is the case of Vietnam, where according to …

A Passion For Haiti, Erin Garrett

University of Mississippi News

Medical mission turns into lifelong commitment for pharmacy and pre-med students

Tethered Domains And Flexible Regions In Trnase Zl, The Long Form Of Trnase Z, Christopher Wilson, Daryl Ramai, Dmitri Serjanov, Neema Lama, Louis Levinger, Emmanuel J. Chang

Publications and Research

tRNase Z, a member of the metallo-β-lactamase family, endonucleolytically removes the pre-tRNA 3′ trailer in a step central to tRNA maturation. The short form (tRNase ZS) is the only one found in bacteria and archaebacteria and is also present in some eukaryotes. The homologous long form (tRNase ZL), exclusively found in eukaryotes, consists of related amino- and carboxy-domains, suggesting that tRNase ZL arose from a tandem duplication of tRNase ZS followed by interdependent divergence of the domains. X-ray crystallographic structures of tRNase ZS reveal a flexible arm (FA) extruded from the body of tRNase Z remote from the active site …

Interview With Jane West - Oh 274, Martha Jane Cato West

Winthrop University Oral History Program

In her July 15, 2013 interview with Martha Manning, Jane Cato West (Class of 1968) details her time at Winthrop as a Biology major in the 1960s. Briefly, West discusses student life and Winthrop traditions, but she provides insight into her life student teaching at Florence High School. Additionally, West includes her experience in required courses like English and Math, as well as experiments conducted in her Biology courses. West concludes the interview with her overall perceptions of Winthrop.

James’S Rule And Causes And Consequences Of A Latitudinal Cline In The Demography Of John’S Snapper (Lutjanus Johnii) In Coastal Waters Of Australia, Mike Cappo, Ross J. Marriott, Stephen J. Newman

Fisheries Research Articles

Demographic parameters were derived from sectioned otoliths of John’s Snapper (Lutjanus johnii) from 4 regions across 9° of latitude and 23° of longitude in northern Australia. Latitudinal variation in size and growth rates of this species greatly exceeded longitudinal variation. Populations of John’s Snapper farthest from the equator had the largest body sizes, in line with James’s rule, and the fastest growth rates, contrary to the temperature size rule for ectotherms. A maximum age of 28.6 years, nearly 3 times previous estimates, was recorded and the largest individual was 990 mm in fork length. Females grew to a larger mean …

The Rate And Effects Of Spontaneous Mutation On Fitness Traits In The Social Amoeba, Dictyostelium Discoideum, David W. Hall, Sara Fox, Jennie J. Kuzdzal-Fick, Joan E. Strassmann, David C. Queller

Biology Faculty Research

We performed a mutation accumulation (MA) experiment in the social amoeba Dictyostelium discoideum to estimate the rate and distribution of effects of spontaneous mutations affecting eight putative fitness traits. We found that the per-generation mutation rate for most fitness components is 0.0019 mutations per haploid genome per generation or larger. This rate is an order of magnitude higher than estimates for fitness components in the unicellular eukaryote Saccharomyces cerevisiae, even though the base-pair substitution rate is two orders of magnitude lower. The high rate of fitness-altering mutations observed in this species may be partially explained by a large mutational target …

An Integrative Framework For Bayesian Variable Selection With Informative Priors For Identifying Genes And Pathways, Bin Peng, Dianwen Zhu, Bradley P. Ander, Xiaoshuai Zhang, Fuzhong Xue, Frank R. Sharp, Xiaowei Yang

Publications and Research

The discovery of genetic or genomic markers plays a central role in the development of personalized medicine. A notable challenge exists when dealing with the high dimensionality of the data sets, as thousands of genes or millions of genetic variants are collected on a relatively small number of subjects. Traditional gene-wise selection methods using univariate analyses face difficulty to incorporate correlational, structural, or functional structures amongst the molecular measures. For microarray gene expression data, we first summarize solutions in dealing with ‘large p, small n’ problems, and then propose an integrative Bayesian variable selection (iBVS) framework for simultaneously identifying causal …

Not Your Everyday Story Of Cuttlefish, St. Norbert College

St. Norbert College Magazine 2013-2018

On the fast track to superstardom in saltwater aquaria through his work with the fragile cuttlefish, Bret Grasse ’08 has found his methods so successful that he has become the world’s go-to expert in culturing generations of cuttlefish.

Evaluating Itpr-Dependence Of Apoptotic Signaling From The Endoplasmic Reticulum, Agustin Vargas, Jay L. Brewster

Featured Research

Stress within the endoplasmic reticulum (ER) can be induced by misfolded proteins accumulating in the lumen of this organelle. Signaling of ER stress to other parts of the cell results in altered gene expression, physiological adaptation, and with sustained stress, apoptosis (cell suicide). ER stress is often studied with highly toxic compounds that create severe ER stress rapidly, and a condition that is likely not physiologically relevant within an organism. In this study, we examine the apoptotic signaling induced by moderate ER stress, and in particular the inositol 1,4,5-trisphosphate receptor (ITPR). The ITPR regulates Ca2+ release from the ER lumen, …

Lysophosphatidic Acid Stimulates Lymphangiogenesis In Human Lymphatic Endothelial Cells, John Macbeth, Donna Nofziger-Plank

Featured Research

Lymphangiogenesis is the process by which new lymphatic vessels sprout and grow from existing vessels whether under developmental, immunological, or cancerous conditions. Proper lymphatic vessel formation is important in working alongside normal angiogenesis in order to help regulate the body’s tissue fluid as well as aid in immunosurveillance. Various factors regulate lymphangiogenesis such as members of the vascular endothelial growth factor family (VEGF). Another factor that has recently been identified to play a role in lymphangiogenesis is the bio-active phospholipid lysophosphatidic acid (LPA) however the molecular mechanism by which LPA regulates lymphangiogenesis has not been well characterized. In this study, …

Leaf Mechanical Strength Corresponds To Water Relations In Twelve Species Of California Ferns, Breahna Gillespie

Featured Research

Mentor: Stephen D. Davis

In angiosperms and gymnosperms, mechanically strong leaves are positively correlated with dehydration-tolerance. In general, leaves that are stronger mechanically tend to be evergreen while those that are not are usually mechanically weak and deciduous in response to water stress. Avoiding water stress, especially in a chaparral Mediterranean-type climate, which receives less than 500 mm of water per year, requires energy-intensive adaptation. Ferns residing in the chaparral are presumed to adopt a similar strategy: either they maintain or abscise their pinnae in drought. It was reasoned that ferns with lower water potential and able to survive in …

An Ethnobotanical Approach To Finding Antimicrobial Compounds In Wooly Blue Curls (Trichostema Lanatum) Using A Kirby-Bauer Disc Diffusion Assay, Matthew C. Fleming, P. Matthew Joyner

Featured Research

Plants can be an important source of creativity and production of new drugs. In this study, extracts of wooly blue curls (Trichostema lanatum) were made using DMSO and tested for antimicrobial activity on a panel of bacteria commonly found in separate ecological niches. Wooly blue curls (WBC) was chosen due to its being recorded as a strong disinfectant by the Chumash people. It was found that WBC does exhibit antimicrobial activity against gram positive bacteria and not against gram negative bacteria. However, gram negative bacteria with reduced drug efflux function became susceptible to the WBC extract.

Carbon Black And Titanium Dioxide Nanoparticles Differentially Activate Oxidative Stress And Apoptosis In A549 Human Alveolar Epithelial Cells, Sarah Alvarado, Brianna Manes, Jay L. Brewster

Featured Research

Recent studies have demonstrated that variation between particulate matter compositions have universally adverse effects on cells and living tissues. Carbon black and titanium dioxide are two such particulates that we are continuously exposed to, yet there is limited research to examine the potential deleterious effects on living tissue. The objective of this study is to characterize the effect of carbon black (CB) and titanium dioxide (TiO2) particulates on A549 human alveolar epithelial lung cells. CB and TiO2 powders were dispersed throughout a solution of water and bovine serum albumin by high-powered sonication. The effects of these particulates on A549 cells …

Immunomodulatory Activity Of Sambucus Mexicana And Trichostema Lanatum On Lps Stimulated Raw 264.7 Macrophage Cells, Victoria Hester, P. Matthew Joyner

Featured Research

Chumash medicinal plants Sambucus mexicana (Mexican elderberry) and Trichostema lanatum (woolly blue curls) were tested for immunomodulatory activity. Anti-inflammatory effects were determined by treating LPS induced RAW 264.7 macrophage cells with plant extracts and measuring the levels of cytokines: tumor necrosis factor alpha (TNF-alpha) and interleukin 10 (IL-10). We hypothesized that both plants would exert immunomodulatory activity by reducing the pro-inflammatory production of TNF-alpha or by promoting M2 polarization with a concurrent increase in IL-10 production. At concentration 0.01 mg/mL woolly blue curls and Mexican elderberry demonstrated anti-inflammatory activity by reducing the concentration of TNF-alpha in vitro, while levels of …

The Survivin And Ciap1 Anti-Apoptotic Proteins Are Differentially Downregulated In Response To Endoplasmic Reticulum Stress, Vicki Mercado, Jay L. Brewster

Featured Research

The endoplasmic reticulum (ER) is an organelle tasked with synthesis and transport of 50% of new cellular proteins. Dysfunction within this organelle creates signals for repair, adaptation, and in severe cases, cellular apoptosis. Multiple human diseases have been associated with ER dysfunction, and the activation of apoptosis in important populations of cells. Inhibitor of Apoptosis (IAP) proteins are cytosolic proteins that play an anti-apoptotic role in the cytosol. The relationship between endoplasmic reticulum (ER) stress and the expression/stability of IAPs is not well characterized. The objective of this study was to characterize the affect of ER stress on the expression/stability …

Variation In Mechanical Strength Of Ferns In The Santa Monica And Santa Cruz Mountains, Helen I. Holmlund

Featured Research

Mentor: Stephen D. Davis

In recent years, experts in plant physiology have begun to explore the functional traits of ferns, especially in regards to their tissue-water relations. However, to our knowledge, no scientist had yet examined the relationship between fern biomechanics and physiology. We examined the mechanical properties of fern stipes (stems) and attempted to relate those properties to ecological and physiological traits. Based on our knowledge of fern anatomy, we hypothesized that stipe mechanical strength would not correlate with cavitation resistance as it does in seed-bearing plants. This assertion that mechanical strength will not relate to cavitation resistance begs …

The Regulatory Effect Of Semaphorin 7a On Proliferation And Migration In Human Umbilical Vein Endothelial Cells, Steven R. Flemming, Donna Nofziger-Plank

Featured Research

Semaphorin 7A (SEMA 7A), a factor originally identified as regulating axon growth, has recently been implicated as a pro-angiogenic factor. The molecular mechanisms for this ability to stimulate angiogenesis have not been identified. This study examines if SEMA 7A can have a direct effect on vascular endothelial cells or whether it indirectly induces angiogenesis through stimulation and recruitment of macrophages as has been suggested. Using a human umbilical vein endothelial cells (HUVECs), the ability of SEMA 7A to affect proliferation and migration was examined. HUVECs were exposed to SEMA 7A directly or to conditioned media collected from macrophages exposed to …

Effect Of Shear Stress On Ecnos Expression And Dilation In Soleus Feed Arteries, Blanca Perez, Jay L. Brewster, Jeffrey Jasperse

Featured Research

Shear stress causes artery dilation and increased expression of endothelial cell nitric oxide synthase (ecNOS) in coronary and placental arteries. We sought to determine the importance of shear stress in maintaining normal dilation and normal levels of ecNOS in rat soleus feed arteries (SFA). SFA were isolated from male Sprague-Dawley rats and cannulated for in vitro microscopy (Fig. 6). SFA were exposed to no shear stress, low shear stress, or high shear stress conditions for 4 hours. After 4 hours, endothelium-dependent dilation (acetylcholine: ACh) and endothelium-independent dilation (sodium nitroprusside: SNP) were tested. Arteries were then uncannulated, mRNA was isolated, and …

The Effect Of Shear Stress, Potassium, And Adenosine On Α-1 Adrenergic Vasoconstriction Of Rat Soleus Feed Arteries, Tanner J. Heckle, Jeffrey Jasperse

Featured Research

During exercise, blood flow increases to the working skeletal muscle primarily because of dilation of the arteries and arterioles feeding the muscle. Sympathetic nerve activity also increases during exercise, augmenting the release of the neurotransmitter norepinephrine (NE) at the arterial wall and into the blood. NE acts to constrict blood vessels; however, arteries and arterioles within contracting skeletal muscle dilate despite the increased NE present. This has led to the concept of functional sympatholysis (4), the idea that a chemical released from contracting skeletal muscle interferes with NE signaling. NE acts by binding to adrenergic (alpha and beta) receptors, and …

Effects Of Instructional Model On Student Attitude In An Introductory Biology Laboratory, Stephen M. Rybczynski, Elisabeth E. Schussler

International Journal for the Scholarship of Teaching & Learning

This study assessed student attitude towards reformed laboratories featuring a factorial design of inquiry (IN) and explicit / reflective (ER) pedagogy to foster nature of science understanding. Students in thirty-one lab sections responded to pre and post semester assessments of their confidence, perception of usefulness, and effectance motivation toward the laboratories. Relative change in attitude (RCA) was not significantly different (p>0.05) among the treatments or their interaction for confidence, usefulness, or effectance motivation. Student self-reports (n = 137) of factors that affected their attitude suggested that grades and TAs played a larger role in determining student attitude than the …

Role Of Nucleation In Cortical Microtubule Array Organization: Variations On A Theme, Erica A. Fishel, Ram Dixit

Biology Faculty Research

The interphase cortical microtubules (CMTs) of plant cells form strikingly ordered arrays in the absence of a dedicated microtubule-organizing center. Considerable research effort has focused on activities such as bundling and severing that occur after CMT nucleation and are thought to be important for generating and maintaining ordered arrays. In this review, we focus on how nucleation affects CMT array organization. The bulk of CMTs are initiated from γ-tubulin-containing nucleation complexes localized to the lateral walls of pre-existing CMTs. These CMTs grow either at an acute angle or parallel to the pre-existing CMT. Although the impact of microtubule-dependent nucleation is …

New Pieces Of The Trichinella Puzzle, Edoardo Pozio, Dante S. Zarlenga

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Contrary to our understanding of just a few decades ago, the genus Trichinella now consists of a complex assemblage of no less than nine different species and three additional genotypes whose taxonomic status remains in flux. New data and methodologies have allowed advancements in detection and differentia- tion at the population level which in turn have demonstrably advanced epidemiological, immunological and genetic investigations. In like manner, molecular and genetic studies have permitted us to hypothe- sise biohistorical events leading to the worldwide dissemination of this genus, and to begin crystalising the evolution of Trichinella on a macro scale. The identification …

Systematic Examination Of Infant Size And Growth Metrics As Risk Factors For Overweight In Young Adulthood, Andrew O. Odegaard, Audrey C. Choh, Ramzi W. Nahhas, Bradford Towne, Stefan A. Czerwinski, Ellen W. Demerath

Population and Public Health Sciences Faculty Publications

Objective:To systematically examine infant size and growth, according to the 2006 WHO infant growth standards, as risk factors for overweight status in young adulthood in a historical cohort. Specifically, to assess: Whether accounting for length (weight-for-length) provides a different picture of risk than weight-for-age, intervals of rapid growth in both weight-for-age and weight-for-length metrics, and what particular target ages for infant size and intervals of rapid growth associate most strongly with overweight as a young adult.Patients/Methods:Data analysis of 422 appropriate for gestational age white singleton infants enrolled in the Fels Longitudinal Study. Odds ratios (OR) for overweight and obesity in …