Digital Commons Network^™

Morpholino Gene Knockdown In Adult Fundulus Heteroclitus: Role Of Sgk1 In Seawater Acclimation, Emily G. Notch, Joseph R. Shaw, Bonita A. Coutermarsh, Marisa Dzioba, Bruce A. Stanton

Dartmouth Scholarship

The Atlantic killifish (Fundulus heteroclitus) is an environmental sentinel organism used extensively for studies on environmental toxicants and salt (NaCl) homeostasis. Previous research in our laboratory has shown that rapid acclimation of killifish to seawater is mediated by trafficking of CFTR chloride channels from intracellular vesicles to the plasma membrane in the opercular membrane within the first hour in seawater, which enhances chloride secretion into seawater, thereby contributing to salt homeostasis. Acute transition to seawater is also marked by an increase in both mRNA and protein levels of serum glucocorticoid kinase 1 (SGK1) within 15 minutes of transfer. …

Population Status, Habitat Dependence, And Reproductive Ecology Of Bahama Orioles: A Critically Endangered Synanthropic Species, Melissa R. Price, Valerie A. Lee, William K. Hayes

Faculty Works

Recent elevation of critically endangered Bahama Orioles (Icterus northropi) to species status prompted us to evaluate their population status, habitat use, and breeding ecology. From surveys, we estimated that at least 141 to 254 individuals remain globally, with 90 to 162, 24 to 44, and 27 to 48 individuals remaining on North Andros Island, Mangrove Cay, and South Andros Island, The Bahamas, respectively. Orioles were observed nesting exclusively in anthropogenic habitat (residential and agricultural land), but home ranges also included nearby pine forest and coppice (dry broadleaf forest). Most nests (40 of 46, or 87%) were in nonnative coconut palm …

Changes In Perceived Self-Efficacy And Attitude Toward Science And Teaching Science In Elementary School, Betsy Ann Sullivan

Dissertations

This study was developed in an effort to ascertain if a proposed biological laboratory curriculum as developed and modeled by the instructor would affect the attitudes and perceived self-efficacy towards science, science teaching and ability to learn science of pre-service elementary teachers. Self-regulated learning (SRL) strategies were incorporated as the variation. Attitudinal topics investigated were the perceived ability to learn science and to teach science.

Students in one biology for non-science majors' biology laboratory class at the University of Southern Mississippi participated in this case study. The group participated in the modified laboratory section which utilized SRL activities, including reflections …

Effects Of Transforming Growth Factor Beta1 On Hantavirus Cardiopulmonary Syndrome, Stephanie Flynn James

Dissertations

No abstract provided.

Impaired Structural Connectivity Of Socio-Emotional Circuits In Autism Spectrum Disorders: A Diffusion Tensor Imaging Study, Stephanie H. Ameis, Jin Fan, Conrad Rockel, Aristotle N. Voineskos, Nancy J. Lobaugh, Latha Soorya, A. Ting Wang, Eric Hollander, Evdokia Anagnostou

Publications and Research

Background Abnormal white matter development may disrupt integration within neural circuits, causing particular impairments in higher-order behaviours. In autism spectrum disorders (ASDs), white matter alterations may contribute to characteristic deficits in complex socio-emotional and communication domains. Here, we used diffusion tensor imaging (DTI) and tract based spatial statistics (TBSS) to evaluate white matter microstructure in ASD. Methods/Principal Findings DTI scans were acquired for 19 children and adolescents with ASD (∼8–18 years; mean 12.4±3.1) and 16 age and IQ matched controls (∼8–18 years; mean 12.3±3.6) on a 3T MRI system. DTI values for fractional anisotropy, mean diffusivity, radial diffusivity and axial …

Cervical Remodeling/Ripening At Term And Preterm Delivery: The Same Mechanism Initiated By Different Mediators And Different Effector Cells, Juan M. Gonzalez, Zhong Dong, Roberto Romero, Guillermina Girardi

Publications and Research

Background Premature cervical remodeling/ripening is believed to contribute to preterm delivery (PTD), the leading cause of perinatal morbidity and mortality. Despite considerable research, the causes of term and PTD remain unclear, and there is no effective treatment for PTD. We previously demonstrated that complement activation plays a causative role in cervical remodeling that leads to PTD in mice. Methodology/Principal Findings Here we found that complement activation is not required for the physiological process that leads to term delivery in mice. Neither increased C3 cervical deposition nor increased C3a and C5a serum levels were observed at term. In addition, macrophages infiltration …

Phosphodiesterase-4 Inhibition Alters Gene Expression And Improves Isoniazid – Mediated Clearance Of Mycobacterium Tuberculosis In Rabbit Lungs, Selvakumar Subbian, Liana Tsenova, Paul O'Brien, Guibin Yang, Mi-Sun Koo, Blas Peixoto, Dorothy Fallows, Veronique Dartois, George Muller, Gilla Kaplan

Publications and Research

Tuberculosis (TB) treatment is hampered by the long duration of antibiotic therapy required to achieve cure. This indolent response has been partly attributed to the ability of subpopulations of less metabolically active Mycobacterium tuberculosis (Mtb) to withstand killing by current anti-TB drugs. We have used immune modulation with a phosphodiesterase-4 (PDE4) inhibitor, CC-3052, that reduces tumor necrosis factor alpha (TNF-α) production by increasing intracellular cAMP in macrophages, to examine the crosstalk between host and pathogen in rabbits with pulmonary TB during treatment with isoniazid (INH). Based on DNA microarray, changes in host gene expression during CC-3052 treatment of Mtb infected …

An Examination Of Laboratory Activities For Elements Of Inquiry In Arkansas Secondary Biology Courses, Michele E. Snyder

Theses and Dissertations

In the last 20 years, several panels recommended improving science teaching by incorporating inquiry approaches into K-12 classrooms. These panels assert that inquiry improves student learning in large part by giving students ownership. However, many teachers still present labs that simply call for students to follow written directions. This cookbook approach is the antithesis of inquiry. To take a "snap shot" of biology teaching, I surveyed Arkansas biology teachers to 1) determine teachers' qualifications, 2) assess the labs that they considered their "best" via a rubric that assesses inquiry activities, and 3) discover the reasons teachers valued certain labs. The …

Ecology, Daisy Wang, Yipsel Ramos

Understanding by Design: Complete Collection

Goals/TEKS:

(10) The student knows that biological systems are composed of multiple levels. The student is expected to:

(C) analyze the levels of organization in biological systems and relate the levels to each other and to the whole system.

(11) The student knows that biological systems work to achieve and maintain balance. The student is expected to:

(B) investigate and analyze how organisms, populations, and communities respond to external factors;

(C) summarize the role of microorganisms in both maintaining and disrupting the health of both organisms and ecosystems

*(D) describe how events and processes that occur during ecological succession can …

Transcriptional Analysis Of Schistosoma Mansoni Treated With Sub-Lethal Doses Of The Anthelmintic Drug Praziquantel, Jarrett Hines-Kay

Biology ETDs

Schistosoma mansoni is one of the most common etiological agents responsible for the disease schistosomiasis. More than 200 million people suffer from this disease making it the most severe tropical disease after malaria in terms of morbidity. Praziquantel (PZQ) is the treatment of choice for schistosomiasis and has been used almost exclusively to treat the disease since the 1970s. However, while the drug is lethal for sexually mature schistosomes, it is ineffective against juveniles. Thus, while morbidity can be eased, a cure is difficult to achieve. As a result there is an urgent need to develop a new generation of …

Mathematical Modeling And Dynamical Analysis Of The Operation Of The Hypothalamus - Pituitary - Thyroid (Hpt) Axis In Autoimmune (Hashimoto's) Thyroiditis, Balamurugan Pandiyan

Dissertations (1934 -)

This thesis is a mathematical modeling study of the operation of the negative feedback control through the hypothalamus-pituitary- thyroid (HPT) axis in autoimmune (Hashimoto's) thyroiditis. Negative feedback control through the HPT axis is a mechanism in which the high levels of thyroid hormone; free thyroxine (FT4) in the blood inhibits the secretion of the pituitary hormone, thyroid stimulating hormone (TSH) into the blood. Similarly, the low levels of free thyroxine (FT4) sensed by the pituitary gland and then it secretes thyroid stimulating hormone (TSH) into the blood. Autoimmune (Hashimoto's) thyroiditis is a disease in which the immune system turns against …

Yeast Bax Inhibitor, Bxi1p, Is An Er-Localized Protein That Links The Unfolded Protein Response And Programmed Cell Death In Saccharomyces Cerevisiae, Nicanor Austriaco, James Cebulski, Joshua Malouin, Nathan Pinches, Vincent Cascio

Biology Faculty Publications

Bax inhibitor-1 (BI-1) is an anti-apoptotic gene whose expression is upregulated in a wide range of human cancers. Studies in both mammalian and plant cells suggest that the BI-1 protein resides in the endoplasmic reticulum and is involved in the unfolded protein response (UPR) that is triggered by ER stress. It is thought to act via a mechanism involving altered calcium dynamics. In this paper, we provide evidence that the Saccharomyces cerevisiae protein encoded by the open reading frame, YNL305C, is a bona fide homolog for BI-1. First, we confirm that yeast cells from two different strain backgrounds lacking YNL305C, …

The Fate Of Alpine Species In The Face Of Climate Change: A Biogeographic Perspective, Madaline Cochrane

Macalester Reviews in Biogeography

Will global climate change affect the spatial distribution and abundance of biodiversity along mountains equally? The elevational gradients inherent as one moves up a mountain slope help define the many biological patterns that emerge as key indicators for species richness in an era of rising global temperatures. When global temperatures arise, the microclimates found along different elevational ranges on mountains also fluctuate. As abiotic characteristics such as daily air temperature, daily precipitation, and annual snowmelt change overtime, so will the biotic communities on a mountain. Some species may be able to adapt to their new environments, while other may have …

Eugenics And Modern Biology: Critiques Of Eugenics, 1910-1945, Garland E. Allen

Biology Faculty Research

Eugenics in most western countries in the first four decades of the twentieth century was based on the idea that genes control most human phenotypic traits, everything from physical features such as polydactyly and eye color to physiological conditions such as the A-B-O blood groups to mental and personality traits such as “feeblemindedness”, alcoholism and pauperism. It assessing the development of the eugenics movement – its rise and decline between 1900 and 1950 – it is important to recognize that its naïve assumptions and often flawed methodologies were openly criticized at the time by scientists and non-scientists alike. This paper …

Mind Perception: Real But Not Artificial Faces Sustain Neural Activity Beyond The N170/Vpp, Thalia Wheatley, Anna Weinberg, Christine Looser, Tim Moran, Greg Hajcak

Dartmouth Scholarship

Faces are visual objects that hold special significance as the icons of other minds. Previous researchers using event-related potentials (ERPs) have found that faces are uniquely associated with an increased N170/vertex positive potential (VPP) and a more sustained frontal positivity. Here, we examined the processing of faces as objects vs. faces as cues to minds by contrasting images of faces possessing minds (human faces), faces lacking minds (doll faces), and non-face objects (i.e., clocks). Although both doll and human faces were associated with an increased N170/VPP from 175–200 ms following stimulus onset, only human faces were associated with a sustained …

Bmp-12 Treatment Of Adult Mesenchymal Stem Cells In Vitro Augments Tendon-Like Tissue Formation And Defect Repair In Vivo, Jonathan Y. Lee, Zuping Zhou, Peter J. Taub, Melissa Ramcharan, Yonghui Li, Takintope Akinbiyi, Edward R. Maharam, Daniel J. Leong, Damien M. Laudier, Takuya Ruike, Phillip J. Torina, Mone Zaidi, Robert Majeska, Mitchell B. Schaffler, Evan L. Flatow, Hui B. Sun

Publications and Research

We characterized the differentiation of rat bone marrow-derived mesenchymal stem cells (BM-MSCs) into tenocyte-like cells in response to bone morphogenetic protein-12 (BMP-12). BM-MSCs were prepared from Sprague-Dawley rats and cultured as monolayers. Recombinant BMP-12 treatment (10 ng/ml) of BM-MSCs for 12 hours in vitro markedly increased expression of the tenocyte lineage markers scleraxis (Scx) and tenomodulin (Tnmd) over 14 days. Treatment with BMP-12 for a further 12-hour period had no additional effect. Colony formation assays revealed that ∼80% of treated cells and their progeny were Scx- and Tnmd-positive. BM-MSCs seeded in collagen scaffolds and similarly treated with a single dose …

A Synthesis Of Plant Invasion Effects On Biodiversity Across Spatial Scales, Kristin I. Powell, Jonathan M. Chase, Tiffany M. Knight

Biology Faculty Research

PREMISE OF THE STUDY:

Invasive plant species are typically thought to pose a large threat to native biodiversity, and local-scale studies typically confirm this view. However, plant invaders rarely cause regional extirpations or global extinctions, causing some to suggest that invasive species' influence on native biodiversity may not be so dire. We aim to synthesize the seemingly conflicting literature in plant invasion biology by evaluating the effects of invasive plant species across spatial scales.

METHODS:

We first conducted a meta-analysis on the effects of invasive plants on the species richness of invaded communities across a range of spatial extents. We …

Lifelines Winter 2011, Southern Adventist University

Lifelines - Biology Department Newsletter

The Winter 2011 issue of Lifelines includes articles on research done by the Ecology class, an alumni feature, the TriBeta National Biological Honor Society, and a pictorial directory of the 2011 biology and allied health graduates.

Quantitatively Measuring In Situ Flows Using A Self-Contained Underwater Velocimetry Apparatus (Scuva), Kakani Katija, Sean Colin, John H. Costello, John O. Dabiri

Arts & Sciences Faculty Publications

The ability to directly measure velocity fields in a fluid environment is necessary to provide empirical data for studies in fields as diverse as oceanography, ecology, biology, and fluid mechanics. Field measurements introduce practical challenges such as environmental conditions, animal availability, and the need for field-compatible measurement techniques. To avoid these challenges, scientists typically use controlled laboratory environments to study animal-fluid interactions. However, it is reasonable to question whether one can extrapolate natural behavior (i.e., that which occurs in the field) from laboratory measurements. Therefore, in situ quantitative flow measurements are needed to accurately describe animal swimming in their natural …

Full Issue: Volume 4, Number 2

The Science Journal of the Lander College of Arts and Sciences

No abstract provided.

Exploring The Open-Close Mechanism Of Dimorphotheca Aurantiaca: Day Versus Night And Cold Versus Hot Temperatures, Tommy Chung, Kathleen Aguilar, Sunny Kim

Featured Research

In the spring of every year on Pepperdine University’s campus, patches of hills are covered with the South African native flower Dimorphotheca aurantiaca or more commonly known as the African Daisy. An observation of the opening and closing mechanism of the flower triggered an important question: does the flower change its shape based on temperature or the presence of light? At night, the African Daisy closes and then opens up again in the daytime. Because temperature has such strong effects on different species of ectodermic animals and temperature-sensitive plants, we hypothesized that the change in temperature initiates the flower’s morphological …

Passing Pains: Revenge, Retaliation, And Redirected Aggression In A New Light, Lixing Sun

Biology Faculty Scholarship

A review of David P. Barash and Judith Eve Lipton, Payback: Why We Retaliate, Redirect Aggression, and Take Revenge, Oxford University Press: New York, 2011, 209 pp., US$24.95, ISBN 019539514X (hardcover).

Existing Regulatory Circuitries Govern Backbone And Acquired Host Association Factors In The Human Pathogen Vibrio Parahaemolyticus, Jennifer C. Mahoney

Doctoral Dissertations

Vibrio parahaemolyticus is a poorly characterized human gastrointestinal pathogen whose virulence mechanisms are not well understood. Though closely related to Vibrio cholerae, V. parahaemolyticus infections are inflammatory and utilize virulence traits that are unique from the Cholera toxins yet remain poorly characterized. Vibrio spp. in general share an extensive core genome dedicated to environmental survival and unique, often horizontally acquired, gene content that is reserved for species specific lifestyles. This diversity has resulted in a genus of highly specialized bacteria partaking in dramatically different lifestyles ranging from symbiosis to pathogenesis. We propose that a comparative genomic and transcriptomic analysis of …

Unraveling The Mystery Of Leaf Reddening In Seagrasses, Alyssa B. Novak

Doctoral Dissertations

Seagrass meadows around the world are declining due to natural and anthropogenic stressors, including global climate change. Recently, more attention has been given to identifying responses that offer resistance to stressors so that researchers can better manage seagrasses for resilience to environmental change. Leaf reddening, the expression of red coloration in leaves, is a well-documented response in terrestrial plants that has been shown to increase resilience to stress, but has been poorly understood in seagrasses. To increase our understanding of the prevalence, causes, and function of leaf in seagrasses, surveys were conducted in the world's six seagrass bioregions and a …

Expression And Characterization Of The Bacterial Mechanosensitive Channel Mscs In Xenopus Laevis Oocytes., Elizabeth S. Haswell, Grigory Maksaev

Biology Faculty Research

We have successfully expressed and characterized mechanosensitive channel of small conductance (MscS) from Escherichia coli in oocytes of the African clawed frog, Xenopus laevis. MscS expressed in oocytes has the same single-channel conductance and voltage dependence as the channel in its native environment. Two hallmarks of MscS activity, the presence of conducting substates at high potentials and reversible adaptation to a sustained stimulus, are also exhibited by oocyte-expressed MscS. In addition to its ease of use, the oocyte system allows the user to work with relatively large patches, which could be an advantage for the visualization of membrane deformation. …

Discovering Conserved Cis-Regulatory Elements That Regulate Expression In Caenorhabditis Elegans, Nnamdi Ihuegbu

All Theses and Dissertations (ETDs)

The aim of this dissertation is two-fold:: 1) To catalog all cis-regulatory elements within the intergenic and intronic regions surrounding every gene in C.elegans: i.e. the regulome) and: 2) to determine which cis-regulatory elements are associated with expression under specific conditions. We initially use PhyloNet to predict conserved motifs with instances in about half of the protein-coding genes. This initial first step was valuable as it recovered some known elements and cis-regulatory modules. Yet the results had a lot of redundant motifs and sites, and the approach was not efficiently scalable to the entire regulome of C. elegans or other …

The Association Between Traumatic Brain Injury And Alzheimer's Disease: Mouse Models And Potential Mechanisms, Hien Tran

All Theses and Dissertations (ETDs)

Alzheimer's disease: AD) is a neurodegenerative disorder characterized pathologically by progressive neuronal loss, extracellular plaques containing the amyloid-β: Aβ) peptides, and neurofibrillary tangles: NFTs) composed of hyperphosphorylated tau proteins. Aβ is thought to act upstream of tau, affecting its phosphorylation and therefore aggregation state. One of the major risk factors for AD is traumatic brain injury: TBI). Acute intra-axonal Aβ and diffuse extracellular plaques occur in approximately 30% of human subjects following severe TBI. Intra-axonal accumulations of total and phospho-tau and less frequently NFTs have also been found in these patients. Due to the lack of an appropriate small animal …

Myosin Vi Regulates Actin Structure Specialization Through Conserved Cargo-Binding Domain Sites, Mamiko Isaji, Marta Lenartowska, Tatsuhiko Noguchi, Deborah J. Frank, Kathryn G. Miller

Biology Faculty Research

Actin structures are often stable, remaining unchanged in organization for the lifetime of a differentiated cell. Little is known about stable actin structure formation, organization, or maintenance. During Drosophila spermatid individualization, long-lived actin cones mediate cellular remodeling. Myosin VI is necessary for building the dense meshwork at the cones' fronts. We test several ideas for myosin VI's mechanism of action using domain deletions or site-specific mutations of myosin VI. The head (motor) and globular tail (cargo-binding) domains were both needed for localization at the cone front and dense meshwork formation. Several conserved partner-binding sites in the globular tail previously identified …

Will The Use Of Less Fecund Cultivars Reduce The Invasiveness Of Perennial Plants?, Tiffany M. Knight, Kayri Havens, Pati Vitt

Biology Faculty Research

Many invasive species were originally introduced for horticultural purposes, and several continue to be profitable for the green (nursery, horticulture, and landscape) industry. Recently, some plant suppliers have marketed less fecund cultivars of several invasive species, including glossy buckthorn (Frangula alnus), burning bush (Euonymus alatus), and Japanese barberry (Berberis thunbergii), as “safe” alternatives to invasive relatives. We use published matrix population models to simulate the effect of reducing fecundity on the population growth rates of invasive species. We show that large changes in fecundity result in relatively small changes to the population growth rates of long-lived species, which suggests that …

Biology Discipline Assessment Report 2010/2011, Biology Discipline

Assessment of Student Learning Reports

No abstract provided.