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Lsu Research Fall 2012, Louisiana State University And Agricultural & Mechanical College

Research Magazine

LSU Research is published annually by the Office of Research & Economic Development, Louisiana State University.

Characterization Of The Stem Cell Niche In Drosophila Testes, Vittorio Mena Jr.

Seton Hall University Dissertations and Theses (ETDs)

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Lesula: A New Species Of Cercopithecus Monkey Endemic To The Democratic Republic Of Congo And Implications For Conservation Of Congo’S Central Basin, John A. Hart, Kate M. Detwiler, Christopher C. Gilbert, Andrew S. Burrell, James L. Fuller, Maurice Emetshu, Terese B. Hart, Ashley Vosper, Eric J. Sargis, Anthony J. Tosi

Publications and Research

In June 2007, a previously undescribed monkey known locally as “lesula” was found in the forests of the middle Lomami Basin in central Democratic Republic of Congo (DRC). We describe this new species as Cercopithecus lomamiensis sp. nov., and provide data on its distribution, morphology, genetics, ecology and behavior. C. lomamiensis is restricted to the lowland rain forests of central DRC between the middle Lomami and the upper Tshuapa Rivers. Morphological and molecular data confirm that C. lomamiensis is distinct from its nearest congener, C. hamlyni, from which it is separated geographically by both the Congo (Lualaba) and the Lomami …

Scimath Course Proposals 09-10-2012, Curriculum Committee

Curriculum Committee Reports

No abstract provided.

Drifters, Sally Leaf

A Midwestern Review: A Magazine of the Arts and Issues at DePauw University

No abstract provided.

Early Invasion Of Brain Parenchyma By African Trypanosomes, Ute Frevert, Alexandru Movila, Olga V. Nikolskaia, Jayne Raper, Zachary B. Mackey, Maha Abdulla, James Mckerrow, Dennis J. Grab

Publications and Research

Human African trypanosomiasis or sleeping sickness is a vector-borne parasitic disease that has a major impact on human health and welfare in sub-Saharan countries. Based mostly on data from animal models, it is currently thought that trypanosome entry into the brain occurs by initial infection of the choroid plexus and the circumventricular organs followed days to weeks later by entry into the brain parenchyma. However, Trypanosoma brucei bloodstream forms rapidly cross human brain microvascular endothelial cells in vitro and appear to be able to enter the murine brain without inflicting cerebral injury. Using a murine model and intravital brain imaging, …

Endoplasmic Reticulum Involvement In Yeast Cell Death, Nicanor Austriaco

Biology Faculty Publications

Yeast cells undergo programed cell death (PCD) with characteristic markers associated with apoptosis in mammalian cells including chromatin breakage, nuclear fragmentation, reactive oxygen species generation, and metacaspase activation. Though significant research has focused on mitochondrial involvement in this phenomenon, more recent work with both Saccharomyces cerevisiae and Schizosaccharomyces pombe has also implicated the endoplasmic reticulum (ER) in yeast PCD. This minireview provides an overview of ER stress-associated cell death (ER-SAD) in yeast. It begins with a description of ER structure and function in yeast before moving to a discussion of ER-SAD in both mammalian and yeast cells. Three examples of …

Condensin Ii Promotes The Formation Of Chromosome Territories By Inducing Axial Compaction Of Polyploid Interphase Chromosomes, Christopher R. R. Bauer, Tom A. Hartl, Giovanni Bosco

Dartmouth Scholarship

The eukaryotic nucleus is both spatially and functionally partitioned. This organization contributes to the maintenance, expression, and transmission of genetic information. Though our ability to probe the physical structure of the genome within the nucleus has improved substantially in recent years, relatively little is known about the factors that regulate its organization or the mechanisms through which specific organizational states are achieved. Here, we show that Drosophila melanogaster Condensin II induces axial compaction of interphase chromosomes, globally disrupts interchromosomal interactions, and promotes the dispersal of peri-centric heterochromatin. These Condensin II activities compartmentalize the nucleus into discrete chromosome territories and indicate …

Cannabis-Dependence Risk Relates To Synergism Between Neuroticism And Proenkephalin Snps Associated With Amygdala Gene Expression: Case-Control Study, Didier Jutras-Aswad, Michelle M. Jacobs, Georgia Yiannoulos, Panos Roussos, Panos Bitsios, Yoko Nomura, Xun Liu, Yasmin L. Hurd

Publications and Research

Background Many young people experiment with cannabis, yet only a subgroup progress to dependence suggesting individual differences that could relate to factors such as genetics and behavioral traits. Dopamine receptor D2 (DRD2) and proenkephalin (PENK) genes have been implicated in animal studies with cannabis exposure. Whether polymorphisms of these genes are associated with cannabis dependence and related behavioral traits is unknown. Methodology/Principal Findings Healthy young adults (18–27 years) with cannabis dependence and without a dependence diagnosis were studied (N = 50/group) in relation to a priori-determined single nucleotide polymorphisms (SNPs) of the DRD2 and PENK genes. Negative affect, Impulsive Risk …

Multi-Locus Phylogeographic And Population Genetic Analysis Of Anolis Carolinensis: Historical Demography Of A Genomic Model Species, Marc Tollis, Gavriel Ausubel, Dhruba Ghimire, Stéphane Boissinot

Publications and Research

The green anole (Anolis carolinensis) has been widely used as an animal model in physiology and neurobiology but has recently emerged as an important genomic model. The recent sequencing of its genome has shed new light on the evolution of vertebrate genomes and on the process that govern species diversification. Surprisingly, the patterns of genetic diversity within natural populations of this widespread and abundant North American lizard remain relatively unknown. In the present study, we use 10 novel nuclear DNA sequence loci (N = 62 to 152) and one mitochondrial locus (N = 226) to delimit green anole populations and …

Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan Greenhagen

Plant and Soil Sciences Faculty Patents

The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.

Minor Fibrillar Collagens, Variable Regions Alternative Splicing, Intrinsic Disorder, And Tyrosine Sulfation, Ming Fang, Reed B. Jacob, Owen Mcdougal, Julia Oxford

Biomedical Research Institute Publications and Presentations

Minor fibrillar collagen types V and XI, are those less abundant than the fibrillar collagens types I, II and III. The alpha chains share a high degree of similarity with respect to protein sequence in all domains except the variable region. Genomic variation and, in some cases, extensive alternative splicing contribute to the unique sequence characteristics of the variable region. While unique expression patterns in tissues exist, the functions and biological relevance of the variable regions have not been elucidated. In this review, we summarize the existing knowledge about expression patterns and biological functions of the collagen types V and …

Isoform-Specific Roles Of Extracellular Signal-Regulated Kinases In Pain, Benedict Alter

All Theses and Dissertations (ETDs)

ABSTRACT OF THE DISSERTATION Isoform-specific roles of Extracellular Signal-Regulated Kinases in pain by Benedict Joseph Alter Doctor of Philosophy in Biology and Biomedical Sciences Neurosciences Washington University in St. Louis, 2012 Professor Robert Gereau, Chairperson The extracellular signal-regulated kinase: ERK) isoforms, ERK1 and ERK2, are believed to be key signaling molecules in nociception and nociceptive sensitization. Studies utilizing inhibitors targeting the shared ERK1/2 upstream activator, mitogen-activated protein kinase kinase: MEK), and transgenic mice expressing a dominant negative form of MEK have established the importance of ERK1/2 signaling. However, these techniques do not discriminate between ERK1 and ERK2. To dissect the …

Inoculation Of Bats With European Geomyces Destructans Supports The Novel Pathogen Hypothesis For The Origin Of White-Nose Syndrome, Lisa L. Warnecke, James M. Turner, Trent K. Bollinger

KIP Articles

No abstract provided.

What Is Life And How Do We Know It? Theological Possibilities In Michael Polanyi's Epistemology, Vincent M. Smiles

Theology Faculty Publications

No abstract provided.

The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz

Honors Theses and Capstones

Six weeks of observation on eight groups of wild mantled howler monkeys revealed that the presence of a dependent infant alters the frequency of social interactions between a female with an infant and other adult monkeys. Males preferred to spend time with females without infants but still spent more time with females with infants than with males. Females without infants spent significantly less time with females with infants than with males or other females without infants and females with infants decreased their frequency of social interaction overall without preference for males, females or other females with infants.

Biology And Pathogenesis Of Acanthamoeba., Ruqaiyyah Siddiqui, Naveed Ahmed Khan

Department of Biological & Biomedical Sciences

Acanthamoeba is a free-living protist pathogen, capable of causing a blinding keratitis and fatal granulomatous encephalitis. The factors that contribute to Acanthamoeba infections include parasite biology, genetic diversity, environmental spread and host susceptibility, and are highlighted together with potential therapeutic and preventative measures. The use of Acanthamoeba in the study of cellular differentiation mechanisms, motility and phagocytosis, bacterial pathogenesis and evolutionary processes makes it an attractive model organism. There is a significant emphasis on Acanthamoeba as a Trojan horse of other microbes including viral, bacterial, protists and yeast pathogens.

Status Of Reintroduced American Marten In The Manistee National Forest Within Michigan’S Northern Lower Peninsula, Julie M. Watkins

McNair Scholars Journal

American martens (Martes americana) were extirpated from the Lower Peninsula of Michigan as a result of overharvest for fur and habitat loss in the early 1900s. More sustainable logging practices, forest regeneration, and improved understanding of wildlife habitat requirements, subsequently led to suitable marten habitat restoration within the Lower Peninsula. In the mid- 1980s in an attempt to re-establish a viable population in the Northern Lower Peninsula, 36 martens were reintroduced into the Manistee National Forest (MNF). In the 2011 summer field season we conducted a pilot study investigating the genetic structure of populations in Ward Hills and Caberfae in …

The Effects Of Water Stress On Datura Wrightii, Tony Festa, Kristin Lapointe, Sara Tandon

Featured Research

The Santa Monica Mountains are home to a unique Mediterranean type ecosystem. Due to the effects of global warming and human disruption, the native species are beginning to decrease in population. It is imperative to observe and study the native species in order to preserve the local plant and animal life. This project focuses on the native flower Datura wrightii, specifically the causes of the opening and closing of its flowers. For Datura wrightii to be pollinated, it must be open. The results of this project will help to educate the public on how to sustain an environment in which …

This Paper Is Only Skin Deep: The Socio-Cultural And Biological Reality Of Human Variation, Paige Jamieson

A with Honors Projects

This project is an anthropological study of human variation and "race".

The Impact Of Seasonal Movements By Ovigerous American Lobsters (Homarus Americanus) On Egg Development And Larval Release, Jason Seth Goldstein

Doctoral Dissertations

The American lobster (Homarus americanus) supports one of the most economically successful fisheries in the Gulf of Maine. The continued success of this fishery is attributed in part to vigilant broodstock conservation through the preservation of ovigerous (egg-bearing) females. Previous studies of ovigerous lobster movements indicate that some, if not most, display seasonal inshore-to-offshore movement patterns. While it has been assumed that these movements serve to expose eggs to thermal regimes that are optimal for development, this theory has never been rigorously tested. In Chapter 1, I present results from ultrasonic tracking studies designed to determine if lobsters in coastal …

The Role Of Red-Backed Salamanders In Ecosystems, Daniel J. Hocking

Doctoral Dissertations

Ecosystems provide a vast array of services that benefit human societies, which can be divided into provisioning, regulating, cultural, and supporting services. Amphibians provide provisioning services in the form of food and use in medical advances. As one of the major vertebrate groups, amphibians also play an important part in cultures throughout the world. Finally, amphibians can be extremely abundant and play important roles in ecosystem supporting services, including altering both physical structure and ecosystem functions. In aquatic systems, tadpoles can alter sedimentation, water clarity, and filamentous algae growth. Additionally, amphibians affect ecosystem functions, including nutrient cycling, decomposition, and primary …

Tracking Environmental Trends In The Great Bay Estuarine System: An Examination Of Water Quality And Nuisance Macroalgal Blooms, Jeremy C. Nettleton

Doctoral Dissertations

Monitoring macroalgae populations is an effective means of detecting long term water quality changes in estuarine systems. To investigate the environmental status of New Hampshire's Great Bay National Estuarine Research Reserve, this study assessed the abundance/distribution of macrophytes, particularly Gracilaria and Ulva species, relative to eutrophication patterns; compared historical (1970s-1990s) and current algal biomass/cover at several sites; and compared Ulva and Gracilaria tissue N/P content to ambient and historical levels. Nitrogen and phosphorus testing revealed that the estuarine system has become eutrophic, and Ulva and Gracilaria biomass/cover have increased significantly. The percent cover of Ulva species, at seasonal maxima, was …

Ecological Effects Of Ditching And Ditch-Plugging In New England Salt Marshes, Robert E. Vincent

Doctoral Dissertations

Anthropogenic activities in New England salt marshes have altered hydrologic flows in various ways, but unintended consequences from some of these habitat modifications have received little attention. Specifically, ditches have existed on salt marshes for decades, but the effects of these hydrologic alterations are only poorly understood. Ditch-plugging is a more recent methodology used for salt marsh habitat enhancement and mosquito control, but the long-term effects from this management practice are also unclear. I used natural tidal creeks and pools as controls to examine the effects resulting from ditching and plugging, respectively, on hydrology, soil characteristics, marsh surface elevation, plant …

An Investigation Of Non-Spatial Cognitive Abilities In An Asocial Corvid, The Clark's Nutcracker (Nucifraga Columbiana), Jan K. Tornick

Doctoral Dissertations

A great deal of research suggests that the cognitive abilities of birds in the family Corvidae (crows and jays) are comparable in many aspects to that of apes. Scientists have posited competing hypotheses to explain how complex cognitive abilities arise in a species or group of animals. One such hypothesis, the social-intelligence hypothesis, states that the demands of living in a large, dynamic group drive an animal's need for complex cognitive skills. The ecological-intelligence hypothesis, predicts that generalist foragers develop more highly flexible behaviors and a wider cognitive repertoire than specialist foragers. To date, cognitive research on corvids has focused …

The Use Of Multibeam And Split-Beam Echo Sounders For Assessing Biomass And Distribution Of Spring-Spawning Atlantic Cod In The Gulf Of Maine, Christopher William Damon Gurshin

Doctoral Dissertations

This research focused on advancing the application of split-beam and multibeam echo sounding to remotely locate and describe spatial distribution, and to provide a relative measure of abundance of the spring-spawning Atlantic cod (Gadus morhua) in the western Gulf of Maine. Specifically, the main objectives of this research were 1) to test the feasibility of a multibeam echo sounder to detect changes in volume backscatter proportional to incrementally decreasing quantities of cod held in a submerged cage, and to compare results to a split-beam echo sounder; 2) to describe the spatio-temporal distribution and estimate biomass of spring-spawning cod in the …

The Historical Fisheries In The Mediterranean Sea: A Reconstruction Of Trawl Gear, Effort And Trends In Demersal Fish Stocks, Giacomo Chato Osio

Doctoral Dissertations

An extensive search of historical data sources and publications has been carried out in different countries of the Mediterranean. This lead to the construction of the largest compilation of historical fisheries information existing in the Mediterranean region. The goal first here was to quantify historical trawling effort. This shows that Mediterranean demersal communities underwent a much longer and more systematic exploitation than previously thought, very likely the longest known exploitation by means of trawls in Europe and North America. Analysis of the data available for the Catalonian, Italian and French areas showed a clearly emerging pattern: fishing capacity increased in …

The Ecological Role Of Feeding Disturbances Of The Atlantic Horseshoe Crab, Limulus Polyphemus, Wan-Jean Lee

Doctoral Dissertations

This study examined the influence of localized disturbances on the heterogeneity of ecological communities at multiple temporal and spatial scales. Foraging disturbances by the epibenthic predator, Atlantic Horseshoe Crab Limulus polyphemus, on the intertidal mudflats of Great Bay estuary, New Hampshire, USA were used as the study system.

This study overcame methodological hurdles in the study of small localized disturbance over extensive areas of soft-sediments. Using a novel, low-cost technique to monitor Limulus foraging disturbances, Great Bay's tidal flats were found to be critical feeding habitats from late spring till fall. Foraging Limulus disturbed the benthos of Great Bay at …

Eating Quality And Variability In Carotenoid Content And Profiles In Winter Squash And Sweet Potato, Jennifer Bonina Noseworthy

Doctoral Dissertations

A comprehensive study at the New Hampshire Agricultural Experiment Station (NHAES) of 16 winter squash cultigens (Cucurbita spp.), including inbred lines and open pollinated and F1 hybrid cultivars from the University of New Hampshire breeding program, was undertaken to determine dry weight (%DW), soluble solids contents (%SSC) and carotenoid contents of mesocarp tissue, major attributes for determining acceptable eating quality and nutritional content. Data were recorded at harvest, either 40 or 60 days after pollination (DAP), and after 30 and 60 days of storage at 14 °C. Carotenoid contents were determined spectrophotometrically and using high performance liquid chromatography (HPLC).

The …

Irruptive Migration Of Chestnut-Backed Chickadees To Southwestern Idaho, Jay D. Carlisle

Intermountain Bird Observatory Publications and Presentations

I document irruptive movements of the Chestnut-backed Chickadee to Lucky Peak in southwestern Idaho, over 80 km from its regular range. Chestnut-backed Chickadees were captured and/or observed at Lucky Peak in 2000, 2004, and 2008. To evaluate the context for this phenomenon, I also examined data on capture of all chickadees and other irruptive species at Lucky Peak and numbers of irruptive species recorded on Idaho Christmas Bird Counts (CBC) from 1997 to 2011. Though CBCs in the winter of 2004–05 (following the largest movement of Chestnut-backed Chickadees at Lucky Peak in fall 2004) found high numbers of many irruptive …