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Characterization Of A Conserved Transient Receptor Potential Channel Supporting Spermatogenesis In Planarian Flatworms, Haley Nicole Curry

Browse all Theses and Dissertations

The molecular processes underlying the control of external stimuli on development of the reproductive system remain to be understood. The Transient Receptor Potential superfamily of proteins (TRPs) consists of cation channels that respond to external stimuli and are abundant in the somatosensory as well as reproductive systems. Mammalian TRP-Melastatin 3 (TRPM3) channels are activated by heat and the neurosteroid pregnenolone. Here we characterize an ortholog of TRPM3 in the planarian flatworm Schmidtea mediterranea (Smed-TRPM3). Smed-TRPM3 was hypothesized to play a role in germline development due to enriched expression in the reproductive system of sexual planarians. In situ hybridization analysis revealed …

Variation In Available Habitat Impacts The Spatiotemporal Interactions Of A Great Lakes Carnivore Community, Mackenzie E. Rich

Browse all Theses and Dissertations

Interactions between carnivores play a crucial role in regulating lower trophic levels. In order to examine interspecific interactions among Great Lakes carnivores, I used occupancy models to examine habitat selection patterns and quantify interactions between species. I determined temporal patterns of activity for each species and quantified interspecific activity overlap using kernel density estimates. To generate these models and analyze the patterns displayed, I placed 25 cameras across 25 km2 of diverse northern Wisconsin forest. I extracted habitat data from the National Landcover Database using GIS to inform my occurrence models for each detected carnivore species. The only significant influence …

Amphibian Vocalization: Implications Of A Novel Laryngeal Muscle In The Calling Mechanisms Of The TúNgara Frog Engystomops Pustulosus, Amy D. Lagorio

University of the Pacific Theses and Dissertations

The current functional model of the anuran larynx includes four pairs of laryngeal muscles. Their contractions do not account, however, for the behavioral control of call complexity observed in male túngara frogs (Engystomops pustulosus), which optionally add a secondary note with distinct harmonic structure to their advertisement call. Examination of the túngara frog's laryngeal morphology through dissection, microtomography, and resin histology has revealed that the m. dilatator laryngis is divided into two separate bundles (superficial and deep). The superficial bundle closely matches the typical description of the m. dilatator laryngis and is well positioned to open the glottis. …

Prevalence Of Dirofilaria Immitis In Southern California’S Invasive Aedes Species And Native Culiseta Incidens, Zaina Chaban

University of the Pacific Theses and Dissertations

Dirofilaria immitis is a mosquito-borne parasite that causes dog heartworm disease, effecting over 100,000 dogs in the United States each year (CAPC - Parasite Prevalence Maps., 2021). Increased disease reports are commonly attributed to an increase in the local mosquito vector population. Since 2013, dog heartworm disease cases have increased by approximately 21% in the United States as reported by the American Heartworm Society. During the same time, invasive mosquitoes belonging to the genus Aedes have been found in Southern California and spread to other areas. The purpose of our research was to determine the prevalence of Dirofilaria …

Biology Of Binge Eating Related Disorders And Proposal For Integration Into Treatment, Karlyne Morawe

Graduate School of Professional Psychology: Doctoral Papers and Masters Projects

The concerningly low recovery and high cross over rates of eating disorders with binge eating behaviors suggests there might be a missing element in current treatment approaches commonly used to address eating disorders. Research supports the existence of significant biological correlation between disorders that are characterized by binge eating behaviors. This paper examines some of the biological processes in which these disorders show the most significant correlations. These include the organism’s response to caloric restriction, the brain’s response to feeding, the biological drive for reproduction, sensory association to feeding, and habituated responding to the feeding process. Additionally, we will explore …

Ribbon Α-Conotoxin Ktm Exhibits Potent Inhibition Of Nicotinic Acetylcholine Receptors, Leanna A. Marquart, Matthew W. Turner, Lisa R. Warner, Matthew D. King, James R. Groome, Owen M. Mcdougal

Chemistry and Biochemistry Faculty Publications and Presentations

KTM is a 16 amino acid peptide with the sequence WCCSYPGCYWSSSKWC. Here, we present the nuclear magnetic resonance (NMR) structure and bioactivity of this rationally designed α-conotoxin (α-CTx) that demonstrates potent inhibition of rat α3β2-nicotinic acetylcholine receptors (rα3β2-nAChRs). Two bioassays were used to test the efficacy of KTM. First, a qualitative PC12 cell-based assay confirmed that KTM acts as a nAChR antagonist. Second, bioactivity evaluation by two-electrode voltage clamp electrophysiology was used to measure the inhibition of rα3β2-nAChRs by KTM (IC₅₀ = 0.19 ± 0.02 nM), and inhibition of the same nAChR isoform by α-CTx MII (IC₅₀ = …

A Mixed-Methods Study Of Entomology Incorporation In U.S. Secondary Science Instruction, Erin M. Ingram

Department of Entomology: Dissertations, Theses, and Student Research

To encourage understanding and appreciation of insects, entomology education advocates have supported and encouraged K-12 teachers to integrate insects and insect-related content into formal science instruction. However, research examining how and why science teachers incorporate entomology into secondary science courses is limited.

A sequential explanatory mixed-methods research study was conducted to address this gap. The study was conducted in two phases. During the first phase, quantitative survey research was conducted with a representative sample of 254 U.S. secondary life science teachers. During the subsequent qualitative phase, follow-up interviews were conducted with a purposeful sample of 18 survey participants and an …

Mammal Species Inventory Using Various Trapping Methods In Zone 4 Of Billy Barquedier National Park, Belize During Rainy Season, Mersady Redding

Animal Science Undergraduate Honors Theses

Belize is a small country, but it is extremely ecologically diverse. Based on the few studies conducted in Belize, the abundance of mammals is low but diversity is high. Particular findings note the number and identity of species differed between four sites in the Maya Mountains of Belize, indicating that a data set from a single site is not representative of the Neotropical region. Insufficient data is available to estimate current species richness of many areas in Belize, including Billy Barquedier National Park (BBNP). The objective of this study was to explore trapping and documentation methods of terrestrial mammals in …

Isolation, Identification, And Antibiotics Susceptibility Determination Of Proteus Species Obtained From Various Clinical Specimens In Erbil City, Treska Dhia Kamil, Sanaria Fawzi Jarjes

Polytechnic Journal

Fifty-two Proteus isolates, (47) Proteus mirabilis, (4) Proteus vulgaris, and (1) Proteus hauseri are being isolated from (200) clinical specimens taken from patients admitted to different hospitals in Erbil city/Kurdistan region of Iraq. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. All isolates were identified depending on cultural, morphological, biochemical characteristics, and confirmatory VITEK 2 system. Furthermore, VITEK 2 (antibiotic susceptibility testing) panel was used to determine the antibiotic susceptibility of Proteus isolates, and the results showed that all isolates were entirely resistant to tetracycline and tigecycline (100%), but sensitive to meropenem. …

Artful Nature And The Legacy Of Maria Sibylla Merian, Emily N. Roush, Shannon R. Zeltmann, Felicia M. Else, Kay Etheridge, Shannon Egan

Schmucker Art Catalogs

The exhibition Artful Nature and the Legacy of Maria Sibylla Merian celebrates the skills and influences of a remarkable woman from seventeenth-century Europe. Curated by Emily Roush ’21 and Shannon Zeltmann ’21 with the guidance of Professors Kay Etheridge (Biology) and Felicia Else (Art History), Emily and Shannon selected the prints, organized them into categories, and carried out research on them, much of which was relatively obscure and would have been challenging even for graduate students.

Maria Sibylla Merian lived and worked in a time of vibrant intersections of art and science in Europe. Her images of insects and plants …

Human Biology Oer, Bio 1100, Course Outline, Tatiana Voza

Open Educational Resources

This course will include selected biological concepts, including the chemical basis of life, cell structure and division, a broad survey of the major systems of the human body with a special emphasis on human health disease, human evolution and ecology. This course should provide students who do not plan to continue in the sciences or pre-health programs with a working knowledge of life science that will be useful in making informed decisions on health and the environment

9th Annual Postdoctoral Science Symposium, University Of Texas Md Anderson Cancer Center Postdoctoral Association

Annual Postdoctoral Science Symposium Abstracts

The mission of the Annual Postdoctoral Science Symposium (APSS) is to provide a platform for talented postdoctoral fellows throughout the Texas Medical Center to present their work to a wider audience. The MD Anderson Postdoctoral Association convened its inaugural Annual Postdoctoral Science Symposium (APSS) on August 4, 2011.

The APSS provides a professional venue for postdoctoral scientists to develop, clarify, and refine their research as a result of formal reviews and critiques of faculty and other postdoctoral scientists. Additionally, attendees discuss current research on a broad range of subjects while promoting academic interactions and enrichment and developing new collaborations.

Newsroom, Georgia Southern University

Newsroom (2006-2023)

• Georgia Southern launches new online IT degree
• Georgia Southern graduate student conducts research as snake specialist in Honduras
• Georgia Southern school psychology students work with special needs children in Ecuador, experience is life-changing
• Georgia Southern's annual economic impact continues to grow, topping more than $1 billion

Trophic Upgrading And Mobilization Of Wax Esters In Microzooplankton, Keyana Roohani, Brad A. Haubrich, Kai-Lou Yue, Nigel D'Souza, Amanda Mantalbano, Tatiana Rynearson, Susanne Menden-Deuer, Christopher Reid

Science and Technology Department Faculty Journal Articles

Heterotrophic protists play pivotal roles in aquatic ecosystems by transferring matter and energy, including lipids, from primary producers to higher trophic predators. Using Oxyrrhis marina as a model organism, changes to the non-saponifiable protist lipids were investigated under satiation and starvation conditions. During active feeding on the alga Cryptomonas sp., the O. marina hexane soluble non-saponifiable fraction lipid profile reflected its food source with the observed presence of long chain mono-unsaturated fatty alcohols up to C25:1. Evidence of trophic upgrading in O. marina was observed with long chain mono-unsaturated fatty alcohol accumulation of up to C35:1. To the best of …

The Evolutionary Diversity And Biological Function Of Phenazine Metabolite Biosynthesis In Burkholderia Spp, Samuel Hendry

Master's Theses

Burkholderia encompass a group of ubiquitous Gram-negative bacteria that include numerous saprophytes, as well as several species that cause infections in animals, immunocompromised patients, and plants. Some species of Burkholderia produce colored redox-active secondary metabolites called phenazines (Phz). In the model opportunistic pathogen Pseudomonas aeruginosa, phenazines strongly contribute to the competitiveness, formation of biofilms, and virulence in multiple models of infection. Similar depth of knowledge on the diversity, biosynthesis, and biological functions of phenazines in Burkholderiais missing. This project aimed to bridge this gap in knowledge by focusing on phenazine pathways of B. lata and closely related …

Summer 2019: Students Solve 33-Million-Year Mini Mystery, St. Norbert College

St. Norbert College Magazine 2019-2023

Two science majors working on fossils of extinct mice have discovered a new way to tell two species apart.

Long-Billed Curlew Nest Site Selection And Success In The Intermountain West, Stephanie E. Coates, Benjamin W. Wright, Jay D. Carlisle

Intermountain Bird Observatory Publications and Presentations

Grassland birds have experienced steeper population declines between 1966 and 2015 than any other bird group on the North American continent, and migratory grassland birds may face threats in all stages of their annual cycle. The grassland‐associated long‐billed curlew (Numenius americanus) is experiencing population declines in regional and local portions of their North American breeding range. The nesting period is an important portion of the annual cycle when curlews may face demographic rate limitations from a suite of threats including predators and anthropogenic disturbance. We compared nest sites to random sites within breeding territories to examine nest site …

Electrostatically Localized Proton Bioenergetics: Better Understanding Membrane Potential, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

In Mitchell's chemiosmotic theory, membrane potential Δψ was given as the electric potential difference across the membrane. However, its physical origin for membrane potential Δψ was not well explained. Using the Lee proton electrostatic localization model with a newly formulated equation for protonic motive force (pmf) that takes electrostatically localized protons into account, membrane potential has now been better understood as the voltage difference contributed by the localized surface charge density ([H-⁺_L] + ⁿΣ _i=1 [M⁽ⁱ⁺⁾_L]) at the liquid-membrane interface as in an electrostatically localized protons/cations-membrane-anions capacitor. That is, the origin of membrane …

A “Rule-Of-Five” Framework For Models And Modeling To Unify Mathematicians And Biologists And Improve Student Learning, C. Diaz Eaton, H. C. Highlander, K. D. Dahlquist, G. Ledder, M.D. Lamar, R.C. Schugart

Department of Mathematics: Faculty Publications

Despite widespread calls for the incorporation of mathematical modeling into the undergraduate biology curriculum, there is lack of a common understanding around the definition of modeling, which inhibits progress. In this paper, we extend the “Rule-of-Four,” initially used in calculus reform efforts, to a “Rule-of-Five” framework for models and modeling that is inclusive of varying disciplinary definitions of each. This unifying framework allows us to both build on strengths that each discipline and its students bring, but also identify gaps in modeling activities practiced by each discipline. We also discuss benefits to student learning and interdisciplinary collaboration.

5th Grade Adaptation, Michael Carroll

Understanding by Design: Complete Collection

A 10 day lesson plan for teaching students in the 5th grade about Biology; specifically on adaptation, inherited traits, and learned behaviors.

Open Textbook Project [Poster], Sue Ann Gardner

University of Nebraska-Lincoln Libraries: Presentations

Details of a porject undertaken to collaboratively write and produce an open access parasitology textbook for undergraduate and graduate students. The book will be published by Zea Books at the University of Nebraska-Lincoln in 2020 and be available in English and Spanish both online and print-on-demand through lulu.com.

Co-Executive Editors: Scott L. Gardner and Sue Ann Gardner, University of Nebraska-Lincoln. Publisher: Paul Royster/Zea Books, University of Nebraska-Lincoln. Spanish Translator: Yoanna Esquivel Greenwood, University of Nebraska-Lincoln. Project Coordinator: Sue Ann Gardner.

Project website: Concepts in Animal Parasitology.

A Rapid Viability And Drug‑Susceptibility Assay Utilizing Mycobacteriophage As An Indicator Of Drug Susceptibilities Of Anti‑Tb Drugs Against Mycobacterium Smegmatis Mc2 155, Gillian Catherine Crowley, Jim O'Mahony, Aidan Coffey, Riona G. Sayers, Paul D. Cotter

Department of Biological Sciences Publications

Background: A rapid in-house TM4 mycobacteriophage-based assay, to identify multidrug resistance against various anti-tuberculosis drugs, using the fast-growing Mycobacterium smegmatis mc² 155 in a microtiter plate format was evaluated, based on phage viability assays. Methods: A variety of parameters were optimized before the study including the minimum incubation time for the drugs, phage and M. smegmatis mc² 155 to be in contact. An increase in phage numbers over 2 h was indicative that M. smegmatis mc² 155 is resistant to the drugs under investigation, however when phage numbers remained static, M. smegmatis mc² 155 found to …

Sensory Perception, Adrian Rodriguez-Contreras

Open Educational Resources

Different types of sensory systems with their functional modalities will be presented. The biological bases for how these functions are generated and modified will then be described. As vision is the principal means of perception, we will focus in this course most on visual processing. Scientific data will be integrated into the lectures, such that students develop critical skills in analyzing data and proposing hypotheses.

Dna Methods To Understand Genetic Variation Of The Mola Mola Populations In And Near Cape Cod, Jessica Barros

Honors Program Theses and Projects

The ocean sunfish Mola mola is a frequent summer visitor to the waters off of Southeastern Massachusetts and Cape Cod. In late fall, water temperatures drop and some animals strand on area beaches. Stranded animals provide an easily available source of DNA to study the population genetics of this species with the specific goal to learn about genetic variation within and between individuals in this population. This study will continue to identify and characterize microsatellite repeat regions that will be used to study genetic variation. Four new microsatellite regions have been identified and amplified using polymerase chain reaction conditions that …

Ptsd Precipitating From Sexual Abuse And Combat War Exposure And Co-Morbid Disorders Of Chronic Pain, Substance Abuse And Immune Systems, Jessica Anzalone

Honors Program Theses and Projects

Posttraumatic stress disorder (PTSD) is a trauma and stress related disorder that some people develop after exposure to a traumatic life altering event. The person must witness the traumatic event, have the traumatic event occur to a loved one, or experience a traumatic event first hand. According to the publication of the American Psychiatric Association, the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5), the diagnostic criteria for the identity of PTSD must include behavioral symptoms that accompany PTSD in four diagnostic clusters; re-experiencing, avoidance, negative cognitions and mood, and arousal (American Psychological Association, 2018). Two …

Autophagy In Dna-Pkcs Knockout Glioblastoma Cancer Stem Cells, Samantha C. Diulus

Honors Capstones

Glioblastoma multiforme (GBM) is a deadly form of brain cancer, with an expected survival of only a few months after diagnosis without therapy. The current standard of care extends life expectancy by a couple of months due to almost universal recurrence of the tumor. Recurrence of GBM is highly dependent on a radiation-resistant subpopulation of GBM, the cancer stem cells (CSCs). CSCs possess an enhanced ability to repair therapy-induced DNA damage, especially DNA double strand breaks (DSBs). To study the role of DNA damage repair in GBM CSCs, our investigations used a knockout (KO) of an enzyme, DNA PKcs, which …

A Review Of The Global Commercial Cephalopod Fishery, With A Focus On Apparent Expansion, Changing Environments, And Management, Corey Clark

HCNSO Student Capstones

Cephalopods are both important predators and prey in many marine environments and important fishery resources in many countries. The global fishery has expanded almost continuously from landings of 580,000 metric tonnes in 1950 to over 4 m.t. in 2007. Cephalopods are ecological opportunists with highly plastic biological characteristics and varied population dynamics. Nearly all commercially harvested species are short-lived and can reproduce quickly, enabling them to evolve more rapidly under high selection pressure relative to many fish competitors and predators. As a result, they may have the biological means to be successful under conditions of long-term global climate change. This …

Bringing Science To Life In The Fifth-Grade Classroom Through Hands-On Learning, Kelly Gately

Honors Program Theses and Projects

Science helps provide insight into the unknown and provides an outlet for exploration of living things. Children are innately curious about how thing work, and they are eager to ask questions. Science is the perfect outlook for students to fully engage in their inquisitiveness, but yet, many classrooms are skipping over science lessons or choosing to heavily focus on other subjects, such as reading and writing. Science is often taught as an elective, integrated with other subjects or it is only focused on for a short period of time. Unfortunately, when this subject does receive attention, lessons are heavily text-book …

The Role Of Oxytocin On Social Behavior Associated With The Formation Of A Social Pair-Bond In The Socially Monogamous Convict Cichlid (Amatitlania Nigrofasciata), Christopher Garcia

Graduate Theses

The mechanisms for monogamy have evolved several times throughout history across various taxa in accordance with selective pressures. In vertebrates, monogamy is facilitated by the formation and the maintenance of social pair-bonds between mates. Social pair-bonds are a form of selective attachment that require complex neurobiological pathways in order to develop and continue. These neurobiological pathways are often regulated by neuroendocrine mechanisms, such as the release of the two neuroendocrine nonapeptides, oxytocin and arginine vasopressin, in specific parts of the brain or body. These neuroendocrine peptides play a big role in social and sexual behaviors. In prairie voles (Microtus ochrogaster) …

Pyy3-36 Efficacy Is Independent Of Photoperiod But Dependent On Time Of Day, Marissa Maroni

Honors Program Theses and Projects

Approximately 70% of Americans are overweight or obese and studies have shown that poverty-dense American counties are the most prone to obesity (Levine, 2011). Along with diet, the endogenous circadian rhythms have been found to be an important piece of human metabolic health. Circadian disruption in humans, such as jet-lag and shift work, have been linked to metabolic problems such as Type 2 Diabetes Mellitus and Metabolic Syndrome. Since circadian disruption affects many demographics including shift workers and routine business travelers it is imperative to understand the biological mechanism behind the relationship between circadian disruption and weight gain. One potential …