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Revision Of The Ant Genus Crematogaster (Hymenoptera: Formicidae) In North America, Cynthia Ellen Morgan

Open Access Theses & Dissertations

The natural history of thirty-eight species of the genus Crematogaster in North America has been discussed and distribution of specimens mapped. A dichotomous key to the workers of these species has been developed. I hypothesized that the species richness is not as great as previously thought in North America. A taxonomic review of these species has been made with the following changes:

Crematogaster cerasi Fitch 1855 senior synonym of C. browni Buren1968.

C. coarctata Mayr 1870 senior synonym of C. californica Emery 1895, C. mormonum

Emery 1895 and C. colei Buren1968.

C. depilis Wheeler 1919 senior synonym of C. larreae …

Ngasp - The Nematode Genome Annotation Assessment Project, Avril Coghlan, Tristan J. Fiedler, Sheldon J. Mckay, Paul R. Flicek, Todd W. Harris, Darin Blasiar, Lincoln D. Stein

Biomedical Engineering and Sciences Faculty Publications

While the C. elegans genome is extensively annotated, relatively little information is available for other Caenorhabditis species. The nematode genome annotation assessment project (nGASP) was launched to objectively assess the accuracy of protein-coding gene prediction software in C. elegans, and to apply this knowledge to the annotation of the genomes of four additional Caenorhabditis species and other nematodes. Seventeen groups worldwide participated in nGASP, and submitted 47 prediction sets across 10 Mb of the C. elegans genome. Predictions were compared to reference gene sets consisting of confirmed or manually curated gene models from WormBase. Results: The most accurate gene-finders were …

Estimating Total Phosphorus And Total Suspended Solids Loads From High Frequency Data, Amber Spackman Jones

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Frequently measured turbidity was examined as a surrogate for total phosphorus (TP) and total suspended solids (TSS) loads at two locations in the Little Bear River, Utah, USA. Using regression techniques, equations were developed for TP and TSS as functions of turbidity. The equations accounted for censored data, and additional explanatory variables to represent hydrological conditions were considered for inclusion in the equations. By using the resulting surrogate relationships with high frequency turbidity measurements, high frequency estimates of TP and TSS concentrations were calculated. To examine the effect of sampling frequency, reference loads were determined from the concentration records for …

Evolution Of The Holozoan Ribosome Biogenesis Regulon, Seth J. Brown, Michael D. Cole, Albert J. Erives

Dartmouth Scholarship

The ribosome biogenesis (RiBi) genes encode a highly-conserved eukaryotic set of nucleolar proteins involved in rRNA transcription, assembly, processing, and export from the nucleus. While the mode of regulation of this suite of genes has been studied in the yeast, Saccharomyces cerevisiae, how this gene set is coordinately regulated in the larger and more complex metazoan genomes is not understood. Here we present genome-wide analyses indicating that a distinct mode of RiBi regulation co-evolved with the E(CG)-binding, Myc:Max bHLH heterodimer complex in a stem-holozoan, the ancestor of both Metazoa and Choanoflagellata, the protozoan group most closely related to animals. These …

Lis1 And Ndel1 Influence The Timing Of Nuclear Envelope Breakdown In Neural Stem Cells, Sachin Hebbar, Mariano T. Mesngon, Aimee M. Guillotte, Bhavim Desai, Ramses Ayala, Deanna S. Smith

Faculty Publications

Lis1 and Ndel1 are essential for animal development. They interact directly with one another and with cytoplasmic dynein. The developing brain is especially sensitive to reduced Lis1 or Ndel1 levels, as both proteins influence spindle orientation, neural cell fate decisions, and neuronal migration. We report here that Lis1 and Ndel1 reduction in a mitotic cell line impairs prophase nuclear envelope (NE) invagination (PNEI). This dyneindependent process facilitates NE breakdown (NEBD) and occurs before the establishment of the bipolar spindle. Ndel1 phosphorylation is important for this function, regulating binding to both Lis1 and dynein. Prophase cells in the ventricular zone (VZ) …

Biology B.A. Pcas 09/05/2008, Curriculum Committee

Curriculum Committee Reports

No abstract provided.

Biology: Multiple Course Revisions 08/29/2008, Curriculum Committee

Curriculum Committee Reports

No abstract provided.

Biology Minor Pcas 10/29/2008, Curriculum Committee

Curriculum Committee Reports

No abstract provided.

Searching The Biological Literature, John L. Kelland

Public Services Faculty Publications

Table of Contents. Chapter 1. Organization of the Scientific Literature and the Flow of Information in the Sciences. Chapter 2. Primary Sources. Chapter 3. Reference Sources. Chapter 4. Databases. Chapter 5. Basic Principles of Searching and Search Logic Chapter 6. Citation Indexes and Citation Searching . Chapter 7. The Citation Concept and its Impact on Society Chapter 8. Resources on the World Wide Web Chapter 9. U. S. Government Publications Chapter 10. Lessons Learned Index

Bionormativity And The Construction Of Parenthood, Katharine K. Baker

All Faculty Scholarship

This piece explores the relationship between legal and biological parenthood. It examines how neither history, nor evolutionary biology nor moral philosophy dictate a legal regime in which parenthood must be based on biological connection, but that attraction to a biological (or “bionormative”) regime remains strong. In explaining why, it suggests that much of what attracts people to bionormativity is not biology itself, but the way in which a biological regime constructs parenthood as a private, exclusive and binary enterprise. It is these ancillary qualities of bionormativity that people may care the most about. Today, a variety of forces put pressure …

The Reproductive Ecology Of Graptemys Geographica In The Central Canal, Kati Keppen Rush

Undergraduate Honors Thesis Collection

Life for all organisms involves carefully managing a limited amount of resources. In many cases these resources affect how organisms Jive on a daily basis. In addition to competition from other species, organisms face competition within their own species, populations, and niches. Those individuals that manage their resources effectively will increase their chances of survival, reproduction, and the continuation of their genes in the gene pool. Studies that focus on life history characteristics of organisms aim to understand the mechanisms used by organisms to increase the chances that their genes will remain in thegenepoolpasttheirown Iifetime.Thegoal ofthesemechanisms istoensuresurvival and maximize reproduction. …

Reproductive Biology Of The Coyote (Canis Latrans): Integration Of Behavior And Physiology, Debra Anne Carlson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wild Canis species possess a unique suite of reproductive traits including social monogamy, copulatory lock/tie, and biparental care. Females are seasonally monestrous and experience an obligatory pseudopregnancy after spontaneous ovulation. While these characteristics have been ascribed to coyotes, an integrated profile of behavior and physiology has not yet been described. In this study, temporal correlations between steroid hormone levels and socio-sexual mating behaviors were documented, as were changes in vaginal epithelium. Pseudopregnancy was compared to pregnancy by contrasting hormone (progesterone, estradiol, prolactin and relaxin) profiles of unmated females to patterns obtained in alternate years when they bred. Meanwhile, social interactions …

Ua1c7 Departmental Photos, Wku Archives

WKU Archives Collection Inventories

Images showing everyday activities of university departments.

Estimation Of Jollyville Plateau Salamander (Eurycea Tonkawae) Populations Using Surface Counts And Mark-Recapture, Lisa O'Donnell, Andy Gluesenkamp, Chris Herrington

KIP Data Sets and Technical Reports

"The Jollyville Plateau salamander (Eurycea tonkawae) is a perennibranchiate salamander found only in springs, spring runs, and subterranean streams in the Northern Edwards Aquifer northwest of Austin, Texas. This species is currently a candidate for listing under the Endangered Species Act due to threats from urban development throughout its limited range and corresponding negative trends in surface counts at several long-term monitoring sites. During 2007, the City of Austin initiated markrecapture surveys of E. tonkawae populations at three spring sites and has compared the results with surface count surveys, which have been conducted as part of a long-term monitoring program …

Differentially Expressed Genes In Aortic Cells From Atherosclerosis-Resistant And Atherosclerosis-Susceptible Pigeons, Janet Lynn Anderson

Doctoral Dissertations

Representational Difference Analysis (RDA) was used to identify genes that were differentially expressed between White Carneau (WC) and Show Racer (SR) pigeon aortic smooth muscle cells. The gene(s) responsible for atherosclerotic resistance in cultured SR smooth muscle cells (SMC) were hypothesized to be silent or down regulated in the WC. In the reciprocal experiments, it was hypothesized that the gene(s) contributing to the spontaneous atherosclerotic phenotype in cultured WC SMC would not be expressed in the SR.

Total RNA was extracted from primary cultured cells of each breed, converted to cDNA, and compared in four reciprocal RDA experiments. Seventy-four transcripts …

Understanding Polyamine Metabolism Through Transgenic Manipulation In Poplar Suspension Cultures, Sridev Mohapatra

Doctoral Dissertations

Polyamines are low molecular weight aliphatic amines that are obligatory requirements for cell survival and growth. The commonly occurring polyamines in plants are putrescine, spermidine, and spermine. Suspension cultures of poplar (Populus nigra x maximowiczii), transformed with a mouse ornithine decarboxylase gene (under the control of a 2X 35S CaMV promoter) were used to study the impact of up-regulation of putrescine biosynthesis (and consequent enhanced catabolism) on several aspects of cellular metabolism. The transgenic cells were compared with a control cell line that was transformed with the beta-glucuronidase (GUS) gene.

It was observed that enhanced putrescine metabolism resulted in: (i) …

Evaluation Of An Enhanced Magneto-Chemical Process For The Removal Of Pathogens In Wastewater, Christine N. Wright

Doctoral Dissertations

New wastewater treatment technologies are a necessity as a result of increasingly stringent discharge standards, particularly concerning viruses. The traditional approach to wastewater treatment consists of biological treatment, such as trickling filters, biofilms, and activated sludge. Although these biological processes have been an adequate means of wastewater treatment, they are intrinsically limited by their biological nature. A magneto-chemical process, known as CoMag(TM), has been developed to improve coagulation and solids separation and expand upon conventional wastewater treatment techniques.

The research objective of this project was to develop and evaluate a bench-scale model of the CoMag(TM) process for the removal of …

Systematics And Taxonomy Of Polyclad Flatworms With A Special Emphasis On The Morphology Of The Nervous System, Sigmer Y. Quiroga

Doctoral Dissertations

In a first survey of the Colombian polyclad fauna, a total of 25 species were collected from the rocky littoral of the Tayrona National Park, Santa Marta, Colombia and deposited at the Museo de Historia Natural Marina del INVEMAR. Six species represented first records for Caribbean region. Furthermore, a new combination Phrikoceros mopsus nov. comb, was proposed, and a possible new species of Pleioplana Faubel, 1983 was found. In addition, a new polyclad family Anocellidae was erected and four deep-sea species were described; two species from the North Pacific Ocean, Anocellidus profundus gen. nov. sp. nov. and Oligocladus voightae sp. …

Comparative Embryology And Muscle Development Of Polyclad Flatworms (Platyhelminthes: Rhabditophora), Diana Marcela Bolanos

Doctoral Dissertations

Polyclads belong to the phylum Platyhelminthes, lineage Rhabdotophora, are simple Bilateria, and represent an interesting and useful group for research in developmental biology. Although polyclads, together with catenulids and macrostomids, have been argued to be most closely related to the ancestral flatworm and hold the key to understand the relationship between development and evolution, knowledge of their embryonic development is still scarce and most of the work on spiralian development has focused on mollusks and annelids. In view of polyclad embryonic significance, a comparative study of their embryonic development including several species of direct and indirect developers was performed.

Developing …

Identification And Characterization Of Protein Phosphatase 2a Mutants In Arabidopsis Thaliana, Enhua Wang

Doctoral Dissertations

Reversible protein phosphorylation is a highly regulated process that affects almost all cellular activities. Phosphorylation involves two groups of enzymes: protein kinases and protein phosphatases. Considering the central role of these enzymes in cells, elucidating their function is extremely important. My research focuses on protein phosphatase 2A (PP2A) C subunits. PP2A is one of the most abundant types of serine/threonine phosphatases in all eukaryotic cells. Compared with animals, PP2A function is not well known in plants. Early studies using protein phosphatase inhibitors are not useful to demonstrate the function of individual PP2A subunits. A reverse genetic approach can be helpful …

Survival Strategies Of Eelgrass In Reduced Light, Caroline A. Ochieng

Doctoral Dissertations

Light reduction due to anthropogenic impacts is the most widespread cause of worldwide decline of eelgrass, an ecologically important marine angiosperm whose role in supporting overall coastal ecosystem productivity has been widely recognized. Understanding eelgrass plant and meadow responses to light reduction has therefore received significant research interest over the last 30 years, while managers have sought tools to identify critical thresholds for light availability and predict impacts of human-induced disturbances in order to prevent further eelgrass loss. In the present thesis, a review of some of the literature on light reduction and its effects on eelgrass (i) summarized the …

The Development Of A Modular Integrated Recirculating Aquaculture System Using Porphyra (Nori) For The Bioremediation Of Marine Finfish Effluent, Jennifer Pauline Day

Doctoral Dissertations

It is crucial for the development of the fish aquaculture industry to be managed in a way that provides a reliable, long term source of products without negatively impacting the environment. The simplest form of integrated multi-tropic aquaculture (IMTA) uses a fed component (e.g. finfish) and an extractive component (e.g. seaweed) to remove the inorganic metabolites from finfish aquaculture effluent. In IMTA systems metabolic wastes become nutrients for the other cultured organisms and are incorporated into potentially valuable biomass. A demonstration-scale Modular Integrated Recirculating Aquaculture Systems (MIRAS) was constructed in a greenhouse adjacent to Great Bay Aquaculture, LLC (GBA), Newington, …

Conserving Dolphins And Fishermen: Combining Science And Local Knowledge To Reduce Cetacean Bycatch, Erika A. Zollett

Doctoral Dissertations

Bycatch is the portion of catch that is neither targeted nor retained by fishermen. It threatens the survival of many marine mammal populations globally, and it occurs in nearly every fishing gear type. Despite its widespread occurrence, observations of bycatch are rare, and scientific data on marine mammal bycatch are limited. Difficulties result in developing models that accurately depict the interactions. This study focuses on developing methodology to combine disparate data sources, specifically data from fishery observer programs and interviews of fishermen, to better understand these interactions and to identify effective mitigation measures. As a case study, this research investigates …

Spawning Stock Identification Of Atlantic Cod (Gadus Morhua) In Us Waters Using Pan I And Microsatellite Genetic Markers, Timothy S. Breton

Master's Theses and Capstones

Most Atlantic cod (Gadus morhua) stocks within U.S. waters are currently in decline as a result of overexploitation and fishing pressure from commercial fisheries. A better understanding of the genetic structure of cod populations is essential to identify stocks for successful fisheries management. In this study, the genetic structure of cod from major temporally and geographically distinct spawning grounds in U.S. waters was investigated. Adult and juvenile cod were sampled from aggregations within the Gulf of Maine, Georges Bank, and southern New England waters in cooperation with commercial fishermen and state fishery biologists. Caudal fin clips were collected and analyzed …

A New Perspective: Atlantic Herring (Clupea Harengus) As A Case Study For Time Series Analysis And Historical Data, Emily Klein

Master's Theses and Capstones

This thesis endeavors to develop methods for the historical analysis of a specific species and location to begin understanding fishery patterns and change over time. The main goal was to develop statistical methods to address historical data and provide long-term information on fishery trends and potential relationships between the fishery and outside influences. The Atlantic herring (Clupea harengus) fishery was investigated for underlying patterns and the possible impact of outside variables and events from 1870 to 2007.

In the Gulf of Maine, Atlantic herring (Clupea harengus) provide critical forage for many economically valuable species, while supporting a major New England …

Optimal Science Lab Design: Impacts Of Various Components Of Lab Design On Students’ Attitudes Toward Lab, John Basey, Loren Sackett, Natalie Robinson

International Journal for the Scholarship of Teaching & Learning

Variations in science lab design can differentially impact student learning. Quantification of these differential impacts can be used in modeling – an approach we term “optimal lab design.” In this study we estimated relative influences of six characteristics of lab design on students’ attitudes toward science labs in three different first-year college biology lab courses (USA). We used two end-of-semester surveys. The first had students choose their favorite and least favorite lab and answer questions associated with the six characteristics and their choices. The second had students provide an overall rating of each lab and a rating based on their …

Eelgrass In The Great Bay Estuary, New Hampshire And Maine: Monitoring Eelgrass Decline And Educating Local Students, Nora Thompson Beem

Master's Theses and Capstones

Eelgrass monitoring efforts in the Great Bay Estuary (GBE), New Hampshire and Maine, by the New Hampshire Port Authority Mitigation Project (Chp. II) and Nutrient Pollution Indicator (NPI) testing (Chp. III) both confirmed a recent trend of eelgrass decline within the GBE. The decline has been most noticeable in the mid-estuary, where four major tributaries drain into the GBE. Eelgrass beds in proximity to Portsmouth's wastewater treatment facility have also experienced decline, highlighting the effects of such point sources on the eelgrass population.

In an effort to introduce eelgrass, its recent decline and its role in the ecology of the …

Rapid Identification Of New England Freshwater Copepods Using A Novel Genetic Barcode, Elisah B. Allan

Master's Theses and Capstones

Identification of freshwater calanoid and cyclopoid copepods is limited to adults of certain sexes because morphological keys are mostly based on mature reproductive structures, necessitating an alternate method. Genetic barcodes are an additional tool for distinguishing species using variation in short segments of DNA. I tested the utility of the 28S rDNA D3 expansion segment as a barcode for identifying five species of calanoids and five species of cyclopoids from multiple lakes from New England. Neighbor-joining trees grouped all conspecifics together with high bootstrap support, except for Leptodiaptomus minutus. Comparisons of intra- vs. interspecific variation revealed a barcode gap for …

Use Of Genetic Tagging To Estimate Abundance And Detect Spatial Patterns Of Black Bears In New Hampshire, Stephanie Coster

Master's Theses and Capstones

Abundance estimates for black bears (Ursus americanus) are an important tool for effective management. Recent advancements in DNA technology have enabled genetic tagging mark-recapture population estimates using DNA from hair samples. I conducted a population estimate using genetic tagging in 2 study sites presumed to have different bear densities in northern New Hampshire (Pittsburg and Milan). To test repeatability, I conducted the genetic tagging estimates in 2 consecutive years. I also compared these estimates to those derived from traditional methods used by the New Hampshire Fish and Game Department (NHFG) using hunter harvest and mortality data. I found that the …

Effects Of Vegetated Buffers On Salt Marsh Plant Composition And Groundwater Nitrogen Uptake, Joanne Singfield Glode

Master's Theses and Capstones

Vegetated shoreline buffers are a best management practice to reduce anthropogenic nitrogen influences on estuarine ecology. This study examined the effects of buffers on (1) groundwater chemistry; (2) the salt marsh border plant community (fertilized and control); and (3) Agropyron pungens response to fertilization. All buffer widths (5 to 15 meters) were somewhat effective at removing groundwater nutrients, with greater concentrations of TDN, NO3--N, NH4 +-N, and DOC found in groundwater wells upslope of the buffer. Although on-site manure storage resulted in 30-fold greater groundwater nitrate concentrations (mean 23 mg/L) at the widest buffer, no differences were found in nitrate …