Digital Commons Network^™

Biochemical Enrichment And Biophysical Characterization Of A Taste Receptor For L-Arginine From The Catfish, Ictalurus Puntatus., William Grosvenor, Yuri Kaulin, Andrew I Spielman, Douglas L Bayley, D Lynn Kalinoski, John H Teeter, Joseph G Brand

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: The channel catfish, Ictalurus punctatus, is invested with a high density of cutaneous taste receptors, particularly on the barbel appendages. Many of these receptors are sensitive to selected amino acids, one of these being a receptor for L-arginine (L-Arg). Previous neurophysiological and biophysical studies suggested that this taste receptor is coupled directly to a cation channel and behaves as a ligand-gated ion channel receptor (LGICR). Earlier studies demonstrated that two lectins, Ricinus communis agglutinin I (RCA-I) and Phaseolus vulgaris Erythroagglutinin (PHA-E), inhibited the binding of L-Arg to its presumed receptor sites, and that PHA-E inhibited the L-Arg-stimulated ion conductance …

Nf04-598 Rabies In Nebraska, Annette K. Bredthauer, David R. Smith

University of Nebraska-Lincoln Extension: Historical Materials

Rabies is a deadly virus infection that can attack the central nervous system of most warm blooded animals, including humans. Rabies is a very old disease. The first recorded description of the disease dates from the 23rd century BC in Babylon. It was rampant in Europe in the 19th century and was carried to the Americas by animals on the early ships.

This NebFact describes rabies in animals and explains how people can protect themselves, as well as their pets or livestock from rabies exposure.

Bridge River Site Of Lillooet, British Columbia| The Faunal Assemblage, Jackie L. Orcholl

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Phosphatidylinositol 3-Kinase Signaling Is Involved In Neurogenesis During Xenopus Embryonic Development, Ying Peng, Bing-Hua Jiang, Pai-Hao Yang, Zongxian Cao, Xianglin Shi, Marie C. M. Lin, Ming-Liang He, Hsiang-Fu Kung

Faculty & Staff Scholarship

No abstract provided.

The Eagleville Times, Volume 2, Issue 7, Debbie Ryan

Eagleville Times

No abstract provided.

The Eagleville Times, Volume 2, Issue 2, Debbie Ryan

Eagleville Times

No abstract provided.

A Continuing Whimsical Search For The True Meaning Of The Term “Product” In Products Liability Litigation, Charles E. Cantú

Faculty Articles

More than a decade has elapsed since an initial attempt was made to discern the true meaning of the term product in products liability litigation. At the time, a brief history of events leading up to the adoption of Section 402A of the Restatement (Second) of Torts was outlined, and it was emphasized that what had at first seemed so simple subsequently proved to be somewhat complex.

An examination of cases involving the sales/service transaction, as well as those involving real estate, blood, electricity, component parts, water, computer software, and ideas, sometimes held that what was involved was a product. …

G03-1514 Use Of Feed Contaminated With Fungal (Mold) Toxins (Mycotoxins), Michael P. Carlson, Steve M. Ensley

University of Nebraska-Lincoln Extension: Historical Materials

Mycotoxins are chemicals produced by fungi (molds) under certain conditions. They are not essential for fungal growth or reproduction, and they are toxic to animals or humans. They are naturally occurring chemicals found in grains, food and feed consumed by humans and animals and cannot be completely avoided. Accordingly, we try to control the amount of such chemicals ingested by humans and animals.

The purpose of this NebGuide is to provide guidelines on use of mycotoxin-contaminated feeds.

Manganese Distribution Across The Blood-Brain Barrier. I. Evidence For Carrier-Mediated Influx Of Managanese Citrate As Well As Manganese And Manganese Transferrin, Janelle S. Crossgrove, David D. Allen, Bonny L. Bukaveckas, Susan S. Rhineheimer, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Manganese (Mn) is an essential element and a neurotoxicant. Regulation of Mn movement across the blood–brain barrier (BBB) contributes to whether the brain Mn concentration is functional or toxic. In plasma, Mn associates with water, small molecular weight ligands and proteins. Mn speciation may influence the kinetics of its movement through the BBB. In the present work, the brain influx rates of ⁵⁴Mn²⁺, ⁵⁴Mn citrate and ⁵⁴Mn transferrin (⁵⁴Mn Tf) were determined using the in situ brain perfusion technique. The influx rates were compared to their predicted diffusion rates, which were determined from …

Optimisation Of Bile Production During Normothermic Preservation Of Porcine Livers., Charles J. Imber, Shawn D. St Peter, Inigo Lopez De Cenarruzabeitia, Hugh Lemonde, Mike Rees, Andrew Butler, Peter T. Clayton, Peter J. Friend

Manuscripts, Articles, Book Chapters and Other Papers

Machine perfusion of livers may provide a mechanism for extended preservation of marginal donor organs before transplantation, as well as a method for viability assessment. It has proved possible in a series of experimental porcine liver perfusions to maintain liver viability for up to 72 h. However, a reduction in bile production with associated histological evidence of cholestasis was seen after 10 h of perfusion, damaging the biliary canaliculi during the preservation period and leaving these organs in an unacceptable condition for transplantation. It was proposed that reduction in bile production was the result of a relentless depletion of available …

Hepatic Steatosis And Its Relationship To Transplantation., Charles J. Imber, Shawn D. St Peter, Ashok Handa, Peter J. Friend

Manuscripts, Articles, Book Chapters and Other Papers

Fatty infiltration of the liver is common in the brain-dead donor population and has a strong correlation with primary nonfunction after cold preservation, a condition that is catastrophic to liver transplant recipients. This literature review examines factors associated with the development, diagnosis, quantification, and clinical management of this difficult condition.

G02-1445 Understanding Vaccines, Dicky D. Griffin, Steve Ensley, David R. Smith, Grant Dewell

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide explains the basics of vaccine value, the differences between types of vaccines used in animals, and discusses vaccine selection and vaccination program development.

Vaccines are an important part of disease prevention and control. Like insurance, vaccines come at a cost, including the price of the vaccine, labor to administer the vaccine, localized tissue damage from vaccine injections, and increased metabolic demand of the animal causing potential performance loss during the time the animal is developing a proper immune response. The increased metabolic demand can cause the animal to look depressed and therefore may be confused with illness. This …

Inhibition Of The Splicing Of Glucose-6-Phosphate Dehydrogenase Precursor Mrna By Polyunsaturated Fatty Acids, Huimin Tao, Wioletta Szeszel-Fedorowicz, Batoul Amir-Ahmady, Matthew A. Gibson, Laura P. Stabile, Lisa M. Salati

Faculty & Staff Scholarship

No abstract provided.

Beta-Galactosidase As A Marker Of Ischemic Injury And A Mechanism For Viability Assessment In Porcine Liver Transplantation., Shawn D. St Peter, Charles J. Imber, Inigo Lopez De Cenarruzabeitia, James Mcguire, Tim James, Richard Taylor, Peter J. Friend

Manuscripts, Articles, Book Chapters and Other Papers

Glycohydrolases are a group of enzymes contained predominantly within lysosomes, which are released during Kupffer cell activation or death. One of these, beta-galactosidase, has been proposed as a marker of ischemia-reperfusion injury in the liver because Kupffer cell activation represents a primary event in the injurious reperfusion cascade. In this study, we compared B-galactosidase with more traditional indicators of liver injury and function in a porcine model of liver preservation. Porcine livers were allocated into two groups: group C (n = 5), preserved in University of Wisconsin solution by standard cold storage for 24 hours, and group W (n = …

Srebp-1 Integrates The Actions Of Thyroid Hormone, Insulin, Camp, And Medium-Chain Fatty Acids On Accalpha Transcription In Hepatocytes, Yanqiao Zhang, Liya Yin, F Bradley Hillgartner

Faculty & Staff Scholarship

In chick embryo hepatocytes, activation of acetyl-CoA carboxylase-α (ACCα) transcription by 3,5,3′-triiodothyronine (T3) is mediated by a cis-acting regulatory unit (−101 to −71 bp) that binds the nuclear T3 receptor (TR) and sterol regulatory element-binding protein-1 (SREBP-1). SREBP-1 directly interacts with TR on the ACCα gene to enhance T3-induced transcription. Here, we show that treating hepatocytes with T3 or insulin stimulates a 4-fold increase in the concentration of the mature, active form of SREBP-1. When T3 and insulin are added together, a 7-fold increase in the mature SREBP-1 concentration is observed. Time course studies indicate that the T3-induced increase in …

The Mannose-Sensitive Hemagglutinin Of Vibrio Cholerae Promotes Adherence To Zooplankton, Deborah A. Chiavelli, Jane W. Marsh, Ronald K. Taylor

Dartmouth Scholarship

The bacterium Vibrio cholerae, the etiological agent of cholera, is often found attached to plankton, a property that is thought to contribute to its environmental persistence in aquatic habitats. The V. cholerae O1 El Tor biotype and V. cholerae O139 strains produce a surface pilus termed the mannose-sensitive hemagglutinin (MSHA), whereas V. cholerae O1 classical biotype strains do not. Although V. cholerae O1 classical does not elaborate MSHA, the gene is present and expressed at a level comparable to that of the other strains. Since V. cholerae O1 El Tor and V. cholerae O139 have displaced V. cholerae O1 classical …

Regulation Of The Processing Of Glucose-6-Phosphate Dehydrogenase Mrna By Nutritional Status, Batoul Amir-Ahmady, Lisa M. Salati

Faculty & Staff Scholarship

No abstract provided.

The Homeodomain Proteins Pbx And Meis1 Are Accessory Factors That Enhance Thyroid Hormone Regulation Of The Malic Enzyme Gene In Hepatocytes, Yutong Wang, Liya Yin, F. Bradley Hillgartner

Faculty & Staff Scholarship

No abstract provided.

Thyroid Hormone Stimulates Acetyl-Coa Carboxylase-Α Transcription In Hepatocytes By Modulating The Composition Of Nuclear Receptor Complexes Bound To A Thyroid Hormone Response Element, Yanqiao Zhang, Liya Yin, F. Bradley Hillgartner

Faculty & Staff Scholarship

No abstract provided.

Effects Of Superovulated Heifer Diet Type And Quantity On Relative Mrna Abundances And Pyruvate Metabolism In Recovered Embryos., C Wrenzycki, P De Sousa, E W Overström, R T Duby, D Herrmann, A J Watson, H Niemann, D O'Callaghan, M P Boland

Obstetrics & Gynaecology Publications

This study investigated the effects of quantity and type of diet fed to superovulated donor heifers on molecular and metabolic indices of embryonic development. These effects included the relative abundances of mRNAs for the alpha 1 subunit of Na/K-ATPase and the antioxidant enzyme Cu/Zn-SOD, as well as pyruvate utilization in bovine morulae and blastocysts developed in vivo. Heifers were fed a daily ration of either grass silage and a citrus-beet pulp-based concentrate or grass silage and a barley-based concentrate for 116 days, both at 3 kg per day or ad libitum. In embryos derived from heifers fed the pulp-based diets, …

A Cultivated Taste For Yeast., C Brenner

Kimmel Cancer Center Faculty Papers

The availability of complete genomic sequences of Saccharomyces cerevisiae has catalyzed a cultural change in the practice of yeast biology, providing opportunities to develop high throughput techniques to define protein function, to define drug targets, and to discover and characterize drugs.

Introducing Animals To New Foods, Behave

All Current Publications

This publication explains different ways of successfully introducing animals to new foods.

Why Animals Die From Eating Poisonous Plants, Usu Extension

All Current Publications

If animals can learn which plants are toxic and which are safe, then why do they eat poisonous plants and die?

Global Cns Gene Transfer For A Childhood Neurogenetic Enzyme Deficiency: Canavan Disease., Paola Leone, Christopher G Janson, Scott J Mcphee, Matthew J During

Department of Neurosurgery Faculty Papers

The neurogenetic prototypic disease on which we chose to test our gene therapy strategy is Canavan disease (CD). CD is an autosomal recessive leukodystrophy associated with spongiform degeneration of the brain. At present the disease is uniformly fatal in affected probands. CD is characterized by mutations in the aspartoacylase (ASPA) gene, resulting in loss of enzyme activity. In this review, recent evidence is summarized on the etiology and possible treatments for CD. In particular, we discuss two gene delivery systems representing recent advances in both viral and liposome technology: a novel cationic liposome-polymer-DNA (LPD) complex, DCChol/DOPE-protamine, as well as recombinant …

Pregnant Rat Uterus Expresses High Levels Of The Type 3 Iodothyronine Deiodinase, Valerie Anne Galton, Elena Martinez, Arturo Hernandez, Emily A. St. Germain, Joanne M. Bates, Donald L. St. Germain

Dartmouth Scholarship

Although thyroid hormones are critically important for the coordination of morphogenic processes in the fetus and neonate, premature exposure of the embryo to levels of the hormones present in the adult is detrimental and can result in growth retardation, malformations, and even death. We report here that the pregnant rat uterus expresses extremely high levels of the type 3 iodothyronine deiodinase (D3), which inactivates thyroxine and 3,3′,5-triiodothyronine by 5-deiodination. Both D3 mRNA and activity were present at the implantation site as early as gestational day 9 (E9), when expression was localized using in situ hybridization to uterine mesometrial and antimesometrial …

Protein Production In The Milk Of Genetically Engineered Animals, Katherine M. Bates

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

There are numerous proteins that have potential uses in commercial and scientific applications that are not utilized to their full potential. this is partly because it is not economically feasible to isolate some of these proteins from their natural sources or to produce them using bacterial fermentation methods. The purpose of this research was to target recombinant protein expression to the mammary glands of genetically engineered or transgenic animals. Foreign protein expression has been achieved in the mammary glands of rabbits, sheep, cows, and swine. By using a strong mammary gland promoter and signal peptide fused to the protein, it …

Polyunsaturated Fatty Acids Inhibit The Expression Of The Glucose-6-Phosphate Dehydrogenase Gene In Primary Rat Hepatocytes By A Nuclear Posttranscriptional Mechanism, L P Stabile, S A Klautky, S M Minor, L M Salati

Faculty & Staff Scholarship

No abstract provided.

Endangered And Threatened Animals Of Utah, Terry Messmer, Marilet Zablan, Boyde Blackwell, Athena Menses, Frank Howe, Leo Lentsch, Richard Drake

All Archived Publications

No abstract provided.

Endangered And Threatened Animals Of Utah, Utah State University Extension

All Archived Publications

No abstract provided.

Ec98-148 Grassland Management With Prescribed Fire, John Ortmann, Daniel D. Beran, Robert A. Masters, James L. Stubbendieck

University of Nebraska-Lincoln Extension: Historical Materials

This circular provides an overview of the use of fire in grassland management. It describes the history and importance of fire in the grassland ecosystem, how plants respond to fire, and the uses and potential benefits of prescribed fire. It also summarizes fire planning, and legal and safety considerations. And finally, it provides guidance on some special uses of fire.