Digital Commons Network^™

Inhibition Of Growth Of Toxoplasma Gondii In Cultured Fibroblasts By Human Recombinant Gamma Interferon., E. R. Pfefferkorn, Paul M. Guyre

Dartmouth Scholarship

The growth of Toxoplasma gondii in cultured human fibroblasts was inhibited by recombinant human gamma interferon at concentrations of 8 to 16 U/ml. The interferon was titrated by observing a total inhibition of parasite plaque formation 7 days after infection. Inhibition of the growth of T. gondii in the early days after infection was measured by marked reductions in the incorporation of radioactive uracil, a precursor that can only be used by the parasites. This assay showed that when cells were pretreated with gamma interferon for 1 day and then infected, inhibition of T. gondii growth could be readily detected …

Isolation Of A Collagenase Cdna Clone And Measurement Of Changing Collagenase Mrna Levels During Induction In Rabbit Synovial Fibroblasts., Robert H. Gross, Lynn A. Sheldon, Colin F. Fletcher, Constance E. Brinckerhoff

Dartmouth Scholarship

To facilitate our studies on the mechanisms controlling collagenase production at a molecular level in rabbit synovial fibroblasts, we have constructed a cDNA library using mRNAs isolated from cells induced with crystals of monosodium urate monohydrate. We have screened this library with cDNA probes made from induced and control mRNA populations. From among 30 clones that hybridized preferentially to the induced-cell probe, 4 contained collagenase sequences. The largest, a clone of 650 base pairs, was identified by its ability to hybrid select a mRNA that could be translated in a cell-free system into a product that was precipitable with monospecific …

Inhibition Of Gluconeogenesis And Glycogenolysis By 2,5-Anhydro-D-Mannitol, R L Hanson, R S Ho, J J Wiseberg, R Simpson, E S Younathan, J B Blair

Faculty & Staff Scholarship

No abstract provided.

Competition Between Hydrocarbon And Barbiturate For Spectral Binding To Hepatic Cytochrome P ≃ 450. Inferences Concerning Spin State Of The Enzyme, W. L. Backes, M Means, W. J. Canady

Faculty & Staff Scholarship

No abstract provided.

Iron And The Liver: Acute Effects Of Iron-Loading On Hepatic Heme Synthesis Of Rats. Role Of Decreased Activity Of 5-Aminolevulinate Dehydrase., H L. Bonkowsky, J F. Healey, P R. Sinclair, J F. Sinclair, S I. Shedlofsky, G H. Elder

Dartmouth Scholarship

Acute iron loading of rats, by intraperitoneal administration of iron-dextran (500 mg Fe/kg body wt 18-20 h before killing) decreased by 30% the rate of conversion of 5-amino-[14C]levulinate ([14C]ALA) into heme as measured with a recently described procedure for liver homogenates (1981. Biochem. J. 198: 595-604). The decrease in conversion of labeled ALA into heme caused by iron loading was shown to be due to a 70-80% decrease in activity of ALA dehydrase. The decrease in activity of ALA dehydrase caused by iron loading was not associated with a decrease in hepatic concentrations of GSH, nor could it be reversed …

Evidence For Hydrated Spermidine-Calf Thymus Dna Toruses Organized By Circumferential Dna Wrapping, Kenneth A. Marx, George C. Ruben

Dartmouth Scholarship

In spermidine-condensed calf thymus DNA preparations, torus–shaped condensates were shown by transmission electron microscopy to exist under the hydrated conditions of the freeze fracture experiment. Using extremely low Pt metal deposition levels (9 A Pt/C) high–contrast replicas of the spermidine–DNA toruses were obtained that showed circumferential wrapping of single DNA double helix–size surface fibres. Stereoscopic analysis of high magnification stereomicrographs established some details of the three-dimensional organization of two DNA double helix sections winding circumferentially on the inner surface of one such torus. These measurements demonstrate the usefulness of stereoscopic analysis of these high macromolecular organization magnification. Measurements on a …

Lions & Lionesses, Tigers & Tigresses, Bears & Other Animals: Sellers' Liability For Dangerous Animals, Bruce A. Levin, Michael Spak

Notre Dame Law Review

No abstract provided.

Attitudes Toward Animals In Greco-Roman Antiquity, Liliane Bodson

Attitudes Towards Animals Collection

Both wild and domesticated animals had a direct and wide-ranging role in the life of the ancient Greeks and Romans. The bond between humans and animals which first originated in the economic needs went far beyond strictly practical matters. It did influence and enrich the Classical culture in its major aspects from literature and arts to philosophy and ethics. It also induced people to analyze the main implications of their relationship with "subhuman" creatures. The present paper aims to survey the range of the attitudes they developed about animals. It also examines to what extent they were concerned with the …

A Guide For Keeping Small Animals In The Elementary School Classroom, Bernad Francis Barhorst

Graduate Theses and Dissertations

No abstract provided.

Mechanisms Of K+ Transport In Isolated Turtle Urinary Bladder. Induction Of Active K+ Secretion In A K+-Absorbing Epithelium., R F Husted, P R Steinmetz

SoM Articles

No abstract provided.

Monoclonal Antibodies To Novel Myeloid Antigens Reveal Human Neutrophil Heterogeneity., Edward D. Ball, Robert F. Graziano, Li Shen, Michael W. Fanger

Dartmouth Scholarship

Three cytotoxic murine monoclonal antibodies that recognize myeloid-specific antigens have been produced by immunization with normal human neutrophils or myeloblasts from a patient with acute myelomonocytic leukemia. Two of these, PMN 6 and PMN 29, are specific for neutrophils; the third monoclonal antibody, AML-2-23, is reactive with the majority of normal monocytes as well as a subpopulation of mature neutrophils. Although neutrophils from all individuals tested expressed these antigens, cytofluorographic analysis revealed that the percentage of cells bearing the PMN 6 and AML-2-23 antigens varied among individuals. Significant additional heterogeneity in the density of each antigen among antigen-bearing cells was …

Hair As An Indicator Of Excessive Aluminum Exposure, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

To determine if excessive systemic exposure to aluminum would be reflected in increased aluminum concentration in hair, rabbits were given a series of aluminum lactate injections. Hair was collected before the aluminum lactate administration from the site of injections and twice after the injections from this site as well as from an area adjoining the injection site. Aluminum was determined by flameless atomic absorption analysis of acid-digested samples. The concentration of aluminum in the hair increased after the injections in samples taken at both times from both sites. Considerable variability in hair aluminum was found before excessive exposure, as has …

Proteins Antigenically Related To The Human Erythrocyte Glucose Transporter In Normal And Rous Sarcoma Virus-Transformed Chicken Embryo Fibroblasts., Donald W. Salter, Stephen A. Baldwin, Gustav E. Lienhard, Michael J. Weber

Dartmouth Scholarship

Antibody raised against the purified human erythrocyte glucose transporter specifically precipitated four proteins from normal and Rous sarcoma virus-transformed chicken embryo cells: a major protein of Mr 41,000 and minor proteins of Mr 68,000, 73,000, and 82,000. The Mr 41,000 and 82,000 proteins were found only in a membrane fraction, not in the soluble fraction, and displayed a heterogeneous mobility on NaDodSO4/polyacrylamide gel electrophoresis, suggesting glycosylation. The Mr 41,000 and 82,000 proteins were increased in amount after malignant transformation in direct proportion to the increase in hexose transport rate, and the increase was dependent on the expression of the src …

Genetically Controlled Variation Of “Acid” Beta-Galactosidase Detected In Rattus Norvegicus By Isoelectric Focusing, T C Douglas, K A Kimmel, P E Dawson

Faculty, Staff and Student Publications

Two genetically variant forms of rat "acid" beta-galactosidase were found to differ in isoelectric point and pH dependence, but not in thermostability or sensitivity to inhibition by p-mercuribenzoate (PMB). The results of two backcrosses and an intercross indicated that the isoelectric focusing phenotypes are controlled by two codominant alleles at a single autosomal locus, for which we propose the name Glb-1. No significant linkage between Glb-1 and albino (LG I), brown (LG II), or hooded (LG VI) was observed. Strain-specific differences in total levels of kidney beta-galactosidase were detected, but it is not yet known whether the variation is controlled …

Synthesis Of Low Molecular Weight Heat Shock Peptides Stimulated By Ecdysterone In A Cultured Drosophila Cell Line., Robert C. Ireland, Edward M. Berger

Dartmouth Scholarship

Treatment of Schneider's line 3 Drosophila cells with the steroid hormone ecdysterone rapidly stimulated the synthesis and accumulation of the polypeptide previously designated p7 [Berger, E. M., Ireland, R. C. & Wyss, C. (1980) Somatic Cell Genet. 6, 119-129]. In this report, p7 is identified as the 23,000-dalton heat shock polypeptide (hsp23). In addition to hsp23, the synthesis of the low molecular weight heat shock polypeptides hsp22, hsp26, and hsp27 was also stimulated by ecdysterone, although to different extents. Hybridization of a nick-translated genomic clone containing the hsp23 gene to a total RNA blot showed that ecdysterone stimulation of hsp23 …

The Changing Concept Of Animals As Property, Vincent P. Mccarthy

Attitudes Towards Animals Collection

Enforced and maintained by a legal superstructure that regulated every aspect of a black [slave's] social, political, economic, and religious life, his property status continued until the middle of the nineteenth century when Congress passed the 13th, 14th, and 15th Amendments to the Constitution, which overturned the Dred Scott decision and recognized that a black human being had legally protectible rights.

There are some signs in recent legal decisions that a similar evolution in the status of animals is taking place: judges are beginning to draw distinctions between animals and property.

But can we ever expect that the courts will …

The Economics Of Farm Animal Welfare, A. J. F. Webster

Agribusiness Collection

The number of ways that one can be nice or nasty to animals are legion. This article will consider only one very specific aspect of farm animal welfare, namely, those systems of intensive animal production in which the system itself, irrespective of the quality of the stockmanship within the system, appears to restrict the normal behavior of farm animals to an unacceptable degree. The systems that were considered by the House of Commons Select Committee on Agriculure (1981) include egg production from hens in battery cages, production of veal from calves deprived of solid food and isolated in wooden crates, …

The Roles Of Dna Polymerases Alpha, Beta, And Gamma In Dna Repair Synthesis Induced In Hamster And Human Cells By Different Dna Damaging Agents., M. R. Miller, D. N. Chinault

Faculty & Staff Scholarship

No abstract provided.

Detergent Extraction Of A Presumptive Gating Component From The Voltage-Dependent Sodium Channel, W J. Culp, D T. Mckenzie

Dartmouth Scholarship

A physiologically characterized radiolabeled neurotoxin complex obtained from venom of the scorpion Leiurus quinquestriatus has been used to identify detergent-solubilized presumptive sodium channel components in sucrose gradients. This toxin-binding component is found in extracts prepared from three sources of excitable membrane but appears to be absent from similar extracts prepared from nonexcitable membrane or from Torpedo californica membrane. Procedures that destroy the physiological activity of the Leiurus neurotoxin lead to a corresponding loss of toxin binding to the putative sodium channel component. The major component recognized by the Leiurus toxin sediments at 6.5 S. Scatchard analysis of quantitative binding experiments …

The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross

Dartmouth Scholarship

We have developed a procedure for selectively enriching a mRNA population for inducible sequences. Other than the induced mRNA species, the population of mRNA in control cells is approximately the same as the mRNA population in induced cells. Cytoplasmic mRNA from control cells is bound to oligo (dT)-cellulose and used as a template for reverse transcriptase, the oligo (dT) serving as a primer. After removing the template mRNAs, the cDNA-cellulose column is used to hybridize a population of mRNAs from induced cells. The non-hybridized poly A ⁺ RNAs are greatly enriched in the inducible sequences. We have used this technique …

The Limits Of Legislation In Achieving Social Change, Theodore S. Meth

Laws and Legislation Collection

This paper is about law, not laboratory animals or philosophical ethics. It proceeds from the premise that law is an appropriate, perhaps inevitable, instrument for dealing with ethical issues related to the use of research animals.

History Of Animal Experimentation Control In The U.K., Judith E. Hampson

Laws and Legislation Collection

The legislative control of the use of animals in experiments in the UK lies in the Cruelty to Animals Act of 1876. Animal Welfare groups and individuals in Britain have pressed for reform of this law almost since its inception 105 years ago, and the British government has recently agreed to bring this legislation up to date. Any new or amended legislation could have far-reaching implications, both for laboratory animal welfare and upon the scientific community and is therefore of considerable importance both in this country and overseas. No proper appreciation of the problem would be possible without reference to …

Arrangement Of Subunits In Microtubules With 14 Profilaments, George M. Langford

Biology - All Scholarship

The structure of 14-protofilament microtubules reassembled from dogfish shark brain tubulin was analyzed by high resolution electron microscopy and optical diffraction. The simultaneous imaging of the protofilaments from near and far sides of these tubules produces a moiré pattern with a period of approximately 96 nm. Optical diffraction patterns show that the 5-nm spots that arise from the protofilaments for the two sides of the tubule are not coincident but lie off the equator by a distance of 1/192 nm-1. These data provide evidence that in reassembled microtubules containing 14 protofilaments, the protofilaments are tilted 1.5 degrees with respect to …

Rat Dendritic Cells Function As Accessory Cells And Control The Production Of A Soluble Factor Required For Mitogenic Responses Of T Lymphocytes., Wolfgang E. Klinkert, Jon H. Labadie, James P. O'Brien, Carl F. Beyer, William E. Bowers

Dartmouth Scholarship

Transformation of T lymphocytes, induced by treatment with periodate or with neuraminidase plus galactose oxidase, requires the participation of accessory cells. Procedures were developed for the fractionation of rat lymph node cells, by which most of the lymphocytes can be recovered as a major population of cells that do not respond to mitogenic stimulation unless accessory cells from a separated minor population are added. Further purification led to a 1000-fold overall increase in accessory activity per cell, with a 50-70% yield. The purest preparations were virtually free of macrophages and contained more than 90% typical dendritic cells. Maximum responses occurred …

Cellular Action Of Vasopressin In Medullary Tubules Of Mice With Hereditary Nephrogenic Diabetes Insipidus, Brian A. Jackson, Richard M. Edwards, Heinz Valtin, Thomas P. Dousa

Dartmouth Scholarship

Our previous studies (1974. J. Clin. Invest.54: 753-762.) suggested that impaired metabolism of cyclic AMP (cAMP) may be involved in the renal unresponsiveness to vasopressin (VP) in mice with hereditary nephrogenic diabetes insipidus (NDI). To localize such a defect to specific segments of the nephron, we studied the activities of VP-sensitive adenylate cyclase, cAMP phosphodiesterase (cAMP-PDIE), as well as accumulation of cAMP in medullary collecting tubules (MCT) and in medullary thick ascending limbs of Henle's loop (MAL) microdissected from control mice with normal concentrating ability and from mice with hereditary NDI.

Adenylate cyclase activity stimulated by VP or by …

Ec17-11-80 Nebraska Outdoor Education Series: Plant A Tree, Joe Range, John D. Orr

University of Nebraska-Lincoln Extension: Historical Materials

Not all trees or shrubs can find a home in your yard or apartment. Help your child to discover a home for the tree or shrub he or she grows or receives. The greatest benefit of this Plant-A-Tree project is being close to a living plant. This project will: introduce five tree planters - one from each area of Nebraska, discuss the parts of tree seeds, give examples of how tree seeds travel, discuss preparation of soil, demonstrate how to grow a seedling tree from seed, demonstrate how to transplant a tree, and offer suggestions for activities and exhibits.

Definition Of The Concept Of "Humane Treatment" In Relation To Food And Laboratory Animals, Bernard E. Rollin

Animal Welfare Collection

The very title of this talk makes a suggestion which must be forestalled, namely the idea that laboratory and food animals enjoy some exceptional moral status by virtue of the fact that we use them. In fact, it is extremely difficult to find any morally relevant grounds for distinguishing between food and laboratory animals and other animals and, far more dramatically, between animals and humans. The same conditions which require that we apply moral categories to humans rationally require that we apply them to animals as well. While it is obviously pragmatically impossible in our current sociocultural setting to expect …

Reverence For Life: An Ethic For High School Biology Curricula, George K. Russell

Education Collection

Ethical and pedagogical arguments are presented against the use of animals by high school students in experiments causing pain/suffering/death of the animal. No justification is seen for such experimentation when perfectly valid alternatives, using noninvasive techniques, exist or could be developed. An important concern is the emotional and psychological growth of young people. An overall objective of high school biology curricula must be to assist students in making viable connections with living biological processes and the natural world.

The Vertebrate Animal In High School Biology, Alan M. Beck

Education Collection

Live vertebrates afford opportunities to capture student interest and develop important educational experiences. Humane care and handling of the animals can be one of the most significant aspects of the lesson.

The study of classroom animals could include a wide range of observational and experimental protocols that do not compromise humane or conservational standards while providing background on the basics of science that encourage and prepare the student for continued education. Basic attention to detail and careful supervision will insure humane care of the animals and minimize the possibility of injury to students from bites and infection or discomfort from …

Animals In British Schools: Legal And Practical Problems, Jennifer Remfry

Education Collection

Well-managed, healthy animals can be useful and beneficial aids to the emotional and intellectual development of young people at the primary and secondary levels of education. In Britain, vertebrate animals are not used in schools for experiments which might cause pain, distress or disease. The laws protecting animals are comprehensive but at present it is the Health and Safety at Work Act (1974) which is having the most impact on the keeping of animals in British schools. The practical skills most needed by teachers are in the handling, sexing and humane killing of animals. Training of teachers should include instruction …