Digital Commons Network^™

Metabolic Effect Of 3,3',5'-Triiodothyronine In Cultured Growth Hormone-Producing Rat Pituitary Tumor Cells. Evidence For A Unique Mechanism Of Thyroid Hormone Action., D L. St Germain

Dartmouth Scholarship

Physiologic levels of 3,3',5'-triiodothyronine (rT3) are generally believed to have minimal metabolic effects in the pituitary gland and other tissues. In the present studies, the regulatory role of rT3 and other thyroid hormones on iodothyronine 5'-deiodinase (I5'D) activity was studied in a growth hormone-producing rat pituitary tumor cell line (GH3 cells). I5'D activity was thiol-dependent and displayed nonlinear reaction kinetics suggesting the presence of two enzymatic processes, one having a low Michaelis constant (Km for thyroxine [T4] of 2 nM) and a second with a high Km value (0.9 microM). Growth of cells in hormone-depleted medium resulted in a two- …

Identification Of Sequences In The Herpes Simplex Virus Thymidine Kinase Gene Required For Efficient Processing And Polyadenylation., Charles N. Cole, Terryl P. Stacy

Dartmouth Scholarship

The herpes simplex virus (HSV) type 1 thymidine kinase gene (tk) was resected from its 3' end with BAL 31 exonuclease. Two sets of plasmids were isolated that lacked information distal to the two copies of the hexanucleotide 5'-AATAAA-3' located at the 3' end of the HSV tk gene. The presence of a simian virus 40 origin of DNA replication in each plasmid facilitated analysis of patterns of transcription in transfected Cos-1 monkey cells. Transcription analyses were performed with an S1 nuclease protection assay. Efficient processing and polyadenylation at the normal site still occurred when all sequences more than 44 …

Comparative Study Of Pituitary-Thyroid Hormone Economy In Fasting And Hypothyroid Rats., Donald L. St Germain, Valerie A. Galton

Dartmouth Scholarship

Starvation in laboratory rodents results in significant alterations in thyroid hormone economy characterized by decreased circulating levels of thyroxine (T4) and 3,5,3'-triiodothyronine (T3) and a decline in serum thyrotropin (TSH) concentration. To investigate this apparent paradox, we have compared in fasted and hypothyroid animals the intracellular parameters mediating thyroid hormone action in the anterior pituitary gland. In vitro saturation analysis combined with quantitation of nuclear T3 content by radioimmunoassay allowed for characterization of pituitary nuclear T3 receptors and estimation of the endogenous fractional receptor occupancy. In rats, thyroidectomized 4 wk earlier, the 10-fold increase in serum TSH levels and decline …

American Attitudes Toward And Knowledge Of Animals: An Update, Stephen R. Kellert

Attitudes Towards Animals Collection

The distribution of a typology of basic attitudes toward animals in the American population is explored through personal interviews with 3,107 randomly selected persons in the 48 contiguous states and Alaska. Data is presented on the prevalence of these attitudes in the overall American population and among major social demographic and animal activity groups. In addition, results are presented on Americans' knowledge of animals as well as their species preferences. Finally, information is presented on perceptions of critical wildlife issues including endangered species, predator control, hunting, trapping, marine mammals and wildlife habitat protection.

Attitudes Toward Animals: Age-Related Development Among Children, Stephen R. Kellert

Attitudes Towards Animals Collection

This paper reviews the results of a study of 267 children in the 2nd, 5th, 8th, and 11th grades. A battery of tests was used to examine children's knowledge and attitudes towards animals, and behavioral contacts with animals. A typology of basic attitudes towards animals and appropriate scales was employed. Children's knowledge and attitudes towards animals were also compared to those of adults 18 years of age and over. Major differences occurred among children distinguished by age, sex, ethnicity, and urban/rural residence. Additionally, significant knowledge and attitude variations occurred among diverse animal-related activity groups (e.g., among children who hunted, birdwatched, …

Competition Between Hydrocarbon And Barbiturate For Spectral Binding To Hepatic Cytochrome P ≃ 450. Inferences Concerning Spin State Of The Enzyme, W. L. Backes, M Means, W. J. Canady

Faculty & Staff Scholarship

No abstract provided.

Evidence For Hydrated Spermidine-Calf Thymus Dna Toruses Organized By Circumferential Dna Wrapping, Kenneth A. Marx, George C. Ruben

Dartmouth Scholarship

In spermidine-condensed calf thymus DNA preparations, torus–shaped condensates were shown by transmission electron microscopy to exist under the hydrated conditions of the freeze fracture experiment. Using extremely low Pt metal deposition levels (9 A Pt/C) high–contrast replicas of the spermidine–DNA toruses were obtained that showed circumferential wrapping of single DNA double helix–size surface fibres. Stereoscopic analysis of high magnification stereomicrographs established some details of the three-dimensional organization of two DNA double helix sections winding circumferentially on the inner surface of one such torus. These measurements demonstrate the usefulness of stereoscopic analysis of these high macromolecular organization magnification. Measurements on a …

Monoclonal Antibodies To Novel Myeloid Antigens Reveal Human Neutrophil Heterogeneity., Edward D. Ball, Robert F. Graziano, Li Shen, Michael W. Fanger

Dartmouth Scholarship

Three cytotoxic murine monoclonal antibodies that recognize myeloid-specific antigens have been produced by immunization with normal human neutrophils or myeloblasts from a patient with acute myelomonocytic leukemia. Two of these, PMN 6 and PMN 29, are specific for neutrophils; the third monoclonal antibody, AML-2-23, is reactive with the majority of normal monocytes as well as a subpopulation of mature neutrophils. Although neutrophils from all individuals tested expressed these antigens, cytofluorographic analysis revealed that the percentage of cells bearing the PMN 6 and AML-2-23 antigens varied among individuals. Significant additional heterogeneity in the density of each antigen among antigen-bearing cells was …

Hair As An Indicator Of Excessive Aluminum Exposure, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

To determine if excessive systemic exposure to aluminum would be reflected in increased aluminum concentration in hair, rabbits were given a series of aluminum lactate injections. Hair was collected before the aluminum lactate administration from the site of injections and twice after the injections from this site as well as from an area adjoining the injection site. Aluminum was determined by flameless atomic absorption analysis of acid-digested samples. The concentration of aluminum in the hair increased after the injections in samples taken at both times from both sites. Considerable variability in hair aluminum was found before excessive exposure, as has …

Genetically Controlled Variation Of “Acid” Beta-Galactosidase Detected In Rattus Norvegicus By Isoelectric Focusing, T C Douglas, K A Kimmel, P E Dawson

Faculty, Staff and Student Publications

Two genetically variant forms of rat "acid" beta-galactosidase were found to differ in isoelectric point and pH dependence, but not in thermostability or sensitivity to inhibition by p-mercuribenzoate (PMB). The results of two backcrosses and an intercross indicated that the isoelectric focusing phenotypes are controlled by two codominant alleles at a single autosomal locus, for which we propose the name Glb-1. No significant linkage between Glb-1 and albino (LG I), brown (LG II), or hooded (LG VI) was observed. Strain-specific differences in total levels of kidney beta-galactosidase were detected, but it is not yet known whether the variation is controlled …

The Changing Concept Of Animals As Property, Vincent P. Mccarthy

Attitudes Towards Animals Collection

Enforced and maintained by a legal superstructure that regulated every aspect of a black [slave's] social, political, economic, and religious life, his property status continued until the middle of the nineteenth century when Congress passed the 13th, 14th, and 15th Amendments to the Constitution, which overturned the Dred Scott decision and recognized that a black human being had legally protectible rights.

There are some signs in recent legal decisions that a similar evolution in the status of animals is taking place: judges are beginning to draw distinctions between animals and property.

But can we ever expect that the courts will …

The Roles Of Dna Polymerases Alpha, Beta, And Gamma In Dna Repair Synthesis Induced In Hamster And Human Cells By Different Dna Damaging Agents., M. R. Miller, D. N. Chinault

Faculty & Staff Scholarship

No abstract provided.

Utilization Of Aspen Populus-Tremuloides Trees As A Ruminant Feed Component, M. Singh, L.D. Kamstra

Aspen Bibliography

When traditional roughages are in short supply or high in price, other fibrous feed sources for ruminants should be considered if available at a competitive price. One such fibrous material shown to have potential as a ruminant feed is the aspen tree (Populus tremuloides Michx.) harvested in its entire form to include bark, leaves and trunk. The aspen tree is the most widespread tree species in North America and the least utilized. Estimates exceed 6 million acres of mature trees in an area which would include the Black Hills (58,000 acres), the Great Lakes region and the Rocky …

Cellular Action Of Vasopressin In Medullary Tubules Of Mice With Hereditary Nephrogenic Diabetes Insipidus, Brian A. Jackson, Richard M. Edwards, Heinz Valtin, Thomas P. Dousa

Dartmouth Scholarship

Our previous studies (1974. J. Clin. Invest.54: 753-762.) suggested that impaired metabolism of cyclic AMP (cAMP) may be involved in the renal unresponsiveness to vasopressin (VP) in mice with hereditary nephrogenic diabetes insipidus (NDI). To localize such a defect to specific segments of the nephron, we studied the activities of VP-sensitive adenylate cyclase, cAMP phosphodiesterase (cAMP-PDIE), as well as accumulation of cAMP in medullary collecting tubules (MCT) and in medullary thick ascending limbs of Henle's loop (MAL) microdissected from control mice with normal concentrating ability and from mice with hereditary NDI.

Adenylate cyclase activity stimulated by VP or by …

Ec17-11-80 Nebraska Outdoor Education Series: Plant A Tree, Joe Range, John D. Orr

University of Nebraska-Lincoln Extension: Historical Materials

Not all trees or shrubs can find a home in your yard or apartment. Help your child to discover a home for the tree or shrub he or she grows or receives. The greatest benefit of this Plant-A-Tree project is being close to a living plant. This project will: introduce five tree planters - one from each area of Nebraska, discuss the parts of tree seeds, give examples of how tree seeds travel, discuss preparation of soil, demonstrate how to grow a seedling tree from seed, demonstrate how to transplant a tree, and offer suggestions for activities and exhibits.

Definition Of The Concept Of "Humane Treatment" In Relation To Food And Laboratory Animals, Bernard E. Rollin

Animal Welfare Collection

The very title of this talk makes a suggestion which must be forestalled, namely the idea that laboratory and food animals enjoy some exceptional moral status by virtue of the fact that we use them. In fact, it is extremely difficult to find any morally relevant grounds for distinguishing between food and laboratory animals and other animals and, far more dramatically, between animals and humans. The same conditions which require that we apply moral categories to humans rationally require that we apply them to animals as well. While it is obviously pragmatically impossible in our current sociocultural setting to expect …

Humaneness Supersedes Curiosity, F. Barbara Orlans

Education Collection

Ethical considerations need to be addressed with respect to educational use of animals. Society extends greater latitude in what is permissible to do to an animal in the name of science to a professional research worker than to a high school student. A balance needs to be made of the significance of the expected experimental results, on the one hand, which the ethical costs, (in terms of pain or death to the animal), on the other. A reasonable boundary can be drawn, based on ethical as well as on practical considerations, to exclude invasive procedures on vertebrate animals in high …

Fault Of The Victim: The Limits Of Liability Under Civil Code Articles 2317, 2318, And 2321, Anne T. Lastilla

Louisiana Law Review

No abstract provided.

Three Linked Enzyme Loci In Fishes: Implications In The Evolution Of Vertebrate Chromosomes, D C Morizot, D A Wright, M J Siciliano

Faculty, Staff and Student Publications

A three-point linkage group comprised of loci coding for adenosine deaminase (ADA), glucose-6-phosphate dehydrogenase (G6PDH), and 6-phospho-gluconate dehydrogenase (6PGD) is described in fish of the genus Xiphophorus (Poeciliidae). The alleles at loci in this group were shown to assort independently from the alleles at three other loci--isocitrate dehydrogenase 1 and 2, and glyceraldehyde-3-phosphate dehydrogenase 1. Alleles at the latter three loci also assort independently from each other. Data were obtained by observing the segregation of electrophoretically variant alleles in reciprocal backcross hybrids derived from crosses between either X. helleri guentheri or X. h. strigatus and X. maculatus. The linkage component …

The George-Anne, Georgia Southern University

The George-Anne

• Campus Beauty Pageant To Highlight 'Dreams'
• Tuition Proposal Dropped In House
• One - Meal Plan Called Impractical
• Homecoming Set Jan. 28
• Interested Students To Visit Southern
• New Course Approved
• CUB Offers Weekly Movies
• People at Southern
• Sex Identity Roles Baffle Both Men And Women
• Short Course Examines Cruelty To Animals
• New Fraternity Forming On GSC Campus
• Sell-ebrating Seventy-six
• The Editorial 'We'
• Applauding No Concerts
• Being Lost In The Crowd: The Fragmented Individual
• GSC Students 'Do Time' In Prison For A Grade
• Comics Battle Teen Pregnancy
• Sports
• Eagle Bulletin

Humane Education Programs For Youth (Panel Discussion), Virgil S. Hollis, Sherwood Norman, Jean Mcclure Kelty

Education Collection

Part I - Dr. Virgil S. Hollis

Developing the number of school administrators who are increasingly becoming interested in humaneness and the humane society members who are interested in education, I think, means that all of us must keep close touch with each other although we know very little of each other's field. This exposure to you and your programs in meetings such as this will surely result in a united attack on a mutual problem We need your help. And you need our help. You need help from the group that I represent in education because the many programs …

H. R. 13168, Florence P. Dwyer

Florence Dwyer Congressional Bills 1967

To amend the Public Health Service Act to provide special assistance for the improvement of laboratory animal research facilities; to establish standards for the humane care, handling, and treatment of laboratory animals in departments, agencies, and instrumentalities of the United States and by recipients of grants, awards, and contracts from the United States; to encourage the study and improvement of the care, handling, and treatment and the development of methods for minimizing pain and discomfort of laboratory animals used in biomedical activities; and to otherwise assure humane care, handling, and treatment of laboratory animals; and for other purposes.

H. R. 8517, Florence P. Dwyer

Florence Dwyer Congressional Bills 1967

To protect the public health by amending the Federal Food, Drug, and Cosmetic Act to consolidate certain provisions assuring the safety and effectiveness of new animal drugs, and for other purposes.

Use Of The Legislative Process In Protecting Animals, Hugh Mcnamee

Laws and Legislation Collection

The statements that I shall make in this address are based upon experience gained by me as a lawyer, and as a member of the Ohio House of Representatives. That experience was applied to the successful pioneer attempt to enact the Ohio Rodeo Law of which I was the author and the pilot. Ohio is the first state in which a law has been passed to prohibit certain cruelty practices prevalent in rodeos and thus virtually eliminate them as a medium of public entertainment.

The origin of legislation is in some comprehension of a condition that needs correction by law. …

Some Effects Of Inorganic Fluoride On Plants, Animals, And Man, Delbert A. Greenwood

Faculty Honor Lectures

~~~ in the compounds of fluorine may be traced to one or more of the following factors: ( 1) the consumption of a drinking water containing one part per million of fluorine as a soluble flouride during the period of calcification and eruption of the teeth causes a reduction in the incidence of tooth decay; however, an intake of drinking water containing more than two parts per million of fluorine causes dental fluorosis of varying degrees in man and certain animals; (2) a continuous intake of excessive amounts of fluorine as mineral supplement, in contaminated forage, or the grazing of …

June 1943, William Preston Davies

W. P. Davies' Newspaper Column ('That Reminds Me')

No abstract provided.

The Alleged Contradiction Between Gen. 1:24-27 And 2:19, Alexander Heidel

Concordia Theological Monthly

The first chapter of Genesis, as every Bible student knows, has the animals made first and then man. But the second chapter is commonly held to reverse the order and to place the creation of man before that of the animals. This view is based upon the assumption that Gen. 2:18-25 constitutes a continuous piece of narrative and that the tense of the Hebrew verb with which v.19 opens therefore implies a sequence in the order of time, necessitating the following translation of v.19: "And so the Lord God formed out of the ground all the beast of the field …

March 1940, William Preston Davies

W. P. Davies' Newspaper Column ('That Reminds Me')

No abstract provided.

June 1937, William Preston Davies

W. P. Davies' Newspaper Column ('That Reminds Me')

No abstract provided.

April 1930, William Preston Davies

W. P. Davies' Newspaper Column ('That Reminds Me')

No abstract provided.