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Glanzmann Thrombasthenia. Cooperation Between Sequence Variants In Cis During Splice Site Selection., Ying Jin, Harry C. Dietz, Robert A. Montgomery, William R. Bell, Iain Mcintosh, Barry Coller, Paul F. Bray

Cardeza Foundation for Hematologic Research

Glanzmann thrombasthenia (GT), an autosomal recessive bleeding disorder, results from abnormalities in the platelet fibrinogen receptor, GP(IIb)-IIIa (integrin alpha(IIb)beta3). A patient with GT was identified as homozygous for a G-->A mutation 6 bp upstream of the GP(IIIa) exon 9 splice donor site. Patient platelet GP(IIIa) transcripts lacked exon 9 despite normal DNA sequence in all of the cis-acting sequences known to regulate splice site selection. In vitro analysis of transcripts generated from mini-gene constructs demonstrated that exon skipping occurred only when the G-->A mutation was cis to a polymorphism 116 bp upstream, providing precedence that two sequence variations …

Measuring Hospital Use Without Claims: A Comparison Of Patient And Provider Reports., R E. Clark, S K. Ricketts, G J. Mchugo

Dartmouth Scholarship

We compared the validity of hospital admission and length of stay reports from patients, outpatient providers, and hospitals, and we examined possible sources of error. Data were collected from people enrolled in a randomized trial of treatment for severe mental illness and substance use disorders, from community mental health centers (CMHCs), and from hospitals. Reports for each of the 74 study participants covered two-year time periods beginning and ending at various times between 1989 and 1993. We compared reports from the various sources and constructed a hybrid with data from all three sources. Using parametric and non-parametric statistics, we compared …

Solving Problems By Analogy: The Benefits And Detriments Of Hints And Depressed Moods, Paula T. Hertel, Alicia J. Knoedler

Psychology Faculty Research

In Experiment 1, mildly depressed (dysphoric) and nondysphoric subjects tried to solve logic, problems that were analogous to subsequent target problems; then they attempted target solutions with or wit hour hints in the form of the analogues' themes. Target solutions were impaired by the hints in the nondysphoric group alone. Experiment 2A was a no-training control to verify that transfer did indeed occur. In Experiment 2B, all subjects received hints in the transfer phase; the training phase was either problem oriented (as in Experiment 1) or memory oriented. Again, nondysphoric subjects solved fewerproblems following problem-oriented training than did both dysphoric …

The Long Journey: From Institutionalization To Adult Day Care, Kristin L Kampschroeder

UNLV Retrospective Theses & Dissertations

This thesis will present a field study of an adult day care center in an attempt to establish a new methodology which will address the different types of clients in the recent phenomenon of adult day care settings. Previous research and field study has only focused on adults within institutional settings or on the institutions themselves. Adult day care, on the other hand, has arisen as an alternate form of care which allows for greater flexibility and concern for clients' needs. This thesis, then, in differentiating adult day care from total institutions and in delineating the types of clients, will …

Impact Of Spinal Cord Injury On The Life Roles Of Women., M Claire Quigley

Department of Occupational Therapy Faculty Papers

This qualitative study was conducted to explore and describe the role experience of five women whose lives were disrupted by a traumatic spinal cord injury and who later returned to their communities after completing intensive rehabilitation programs. In-depth interviews and participant observations were used to examine the experiences of these women. The findings exemplify how the women's use of adaptation and negotiation and the development of a new role as self-advocate facilitated the reestablishment of their life roles. As the women's occupational roles were redefined, the processes of adaptation and negotiation were evident in three aspects of their lives: daily …

Mentorship Experiences In A Group Of Occupational Therapy Leaders., Ruth Levine Schemm, Theodore Bross

Department of Occupational Therapy Faculty Papers

OBJECTIVES: The development of occupational therapy leaders is a long-held goal of many members of the American Occupational Therapy Association and of state associations. The initiation of mentor programs is a common means to accomplish this goal. These programs take time and effort, and, although occupational therapists have described how mentorship programs work in the literature, there are few articles that describe the actual mentorship experiences of occupational therapy leaders.

METHOD: To study the experiences of elected occupational therapy leaders, a 30-item questionnaire was distributed at the annual meeting of the Committee of State Association Presidents and completed by 53 …

Epidemiology Of Gambling And Depression In A Random Adult Sample, James A. Thorson, F. C. Powell, Michael L. Hilt

Communication Faculty Publications

Presents a study of the relationship between gambling and depression in a sample of adults in the United States. Absence of relationship.

Comparison Of Cytotoxic Properties Of Neonatal And Adult Neutrophils And Monocytes And Enhancement By Cytokines., E . R. Stiehm, R. L. Roberts, B. J. Ank, S. Plaeger-Marshall, N. Salman, L. Shen, M. W. Fanger

Dartmouth Scholarship

We studied cytotoxic capabilities of newborn polymorphonuclear leukocytes (PMNs) and monocytes and their enhancement by cytokines and antibodies. Umbilical cord PMNs were assessed for their ability to kill various target cells spontaneously, after activation with phorbol myristate acetate, in the presence of antiserum (antibody-dependent cellular cytotoxicity), and in the presence of dually specific antibody (heteroantibody-mediated cytotoxicity). Target cells included the K562 cell line (natural killer cell target), chicken erythrocytes (CRBCs), and herpes simplex virus-infected CEM cell lines. Newborn PMNs were equivalent to adult PMNs in their cytotoxic capacity in several cytotoxicity assays. Neither adult nor newborn PMNs lyse tumor cell …

Elevated Expression Of Type Vii Collagen In The Skin Of Patients With Systemic Sclerosis. Regulation By Transforming Growth Factor-Beta., Lidia Rudnicka, John Varga, Angela M. Christiano, Renato V. Iozzo, Sergio A. Jimenez, Jouni Uitto

Department of Dermatology and Cutaneous Biology Faculty Papers

A hallmark of systemic sclerosis (SSc) is the development of tissue fibrosis. Excessive production of several connective tissue components normally present in the dermis, including type I, III, V, and VI collagens as well as fibronectin and proteoglycans, is a consistent finding in the skin of SSc patients. Type VII collagen is a major constituent of anchoring fibrils, present in the skin at the dermal-epidermal basement membrane zone. TGF-beta has been shown to upregulate the expression of the type VII collagen gene. In this study, we assessed the expression of type VII collagen and TGF-beta in the skin of patients …

Nf94-201 The Child And Adult Care Food Program, Darlene Martin

University of Nebraska-Lincoln Extension: Historical Materials

This NebFact discusses the Child and Adult Care Food Program as administered by the United States Department of Agriculture.

Sequencing Of Cdna From 50 Unrelated Patients Reveals That Mutations In The Triple-Helical Domain Of Type Iii Procollagen Are An Infrequent Cause Of Aortic Aneurysms., Gerard Tromp, Yuli Wu, Darwin J. Prockop, Swarna L. Madhatheri, Caren Kleinert, James J. Earley, Jiapiao Zhuang, Orjan Norrgård, R. Clement Darling, William M. Abbott, C. William Cole, Pekka Jaakkola, Markku Ryynanen, William H. Pearce, James S.T. Yao, Kari Majamaa, Stanton N. Smullens, Zoran Gatalica, Robert E. Ferrell, Sergio A. Jimenez, Charles E. Jackson, Virginia V. Michels, Michael Kaye, Helena Kuivaniemi

Department of Biochemistry and Molecular Biology Faculty Papers

Detailed DNA sequencing of the triple-helical domain of type III procollagen was carried out on cDNA prepared from 54 patients with aortic aneurysms. The 43 male and 11 female patients originated from 50 different families and five different nationalities. 43 patients had at least one additional blood relative who had aneurysms. Five overlapping asymmetric PCR products, covering all the coding sequences of the triple-helical domain of type III procollagen, were sequenced with 28 specific sequencing primers. Analysis of the sequencing gels revealed only two nucleotide changes that altered the structure of the protein. One was a substitution of threonine for …

Regulation Of Human Lung Fibroblast Alpha 1(I) Procollagen Gene Expression By Tumor Necrosis Factor Alpha, Interleukin-1 Beta, And Prostaglandin E2., Arturo Diaz, Elena Munoz, Rosemary Johnston, Joseph H. Korn, Sergio A. Jimenez

Department of Medicine Faculty Papers

We investigated the participation of prostaglandin (PG) E2 in the regulation of the alpha 1(I) procollagen gene expression by tumor necrosis factor alpha (TNF alpha), and interleukin-1 beta (IL-1 beta) in normal adult human lung fibroblasts. TNF alpha (100 units/ml) and IL-1 beta (100 units/ml) stimulated the production of PGE2 and caused a dose-dependent inhibition of up to 54 and 66%, respectively, of the production of type I procollagen. Preincubation of cultures with indomethacin partially reversed the inhibition of procollagen production induced by the cytokines. Cytokine-stimulated endogenous fibroblast PG accounted for 35 and 68% of the inhibition induced by TNF …

The Relationship Between Future Time Perspective And Breast Self-Examination In Young Adult Women, Nancy Marie Ronan

UNLV Retrospective Theses & Dissertations

The purpose of this study was to explore the relationship between future time perspective (FTP) and frequency and quality of BSE performance. FTP relates to the way an individual views her own personal future, which may influence many aspects of her life, including behavior. Recent studies show FTP to positively relate to other health behaviors; The sample consisted of 153 young adult women from the Student Health Center of a large University who took an informational health class which included BSE instruction. A demographic questionnaire, Champion's Quality of BSE survey, and Heimberg's FTP Inventory were used to obtain data. A …

G92-1117 The Sandwich Generation: A Cluttered Nest, Herbert G. Lingren, Jayne Decker

University of Nebraska-Lincoln Extension: Historical Materials

The needs of three generations and solutions to resolve their stresses are covered here.

Lisa and Bruce both have careers. They have a teen-age son, a college student daughter, a recently divorced daughter with a one-year-old child, and an elderly mother who needs an increasing amount of caregiving, all living in the same household. They are classic members of the sandwich generation.

In the 1990s, people live longer, they postpone marriage, more children are living at home to go to college, and an increasing number of adult children are returning home after a divorce or loss of job. Consequently, larger …

The Validity Of Perceived Physical Fitness In Adult Men And Women, Patricia Anne Mortati Mccollum

UNLV Retrospective Theses & Dissertations

This study investigated the perception of personal physical fitness in adult men and women by comparing a Perceived Physical Fitness Index (PPFI) with a composite index of measured fitness. Subjects were 106 volunteer adults 36-55 years of age. They completed the PPFI (a 7-point Likert-type scale) and were then assessed for body composition, cardiorespiratory endurance, muscular strength and endurance, and flexibility. A composite fitness score was compared with their PPFI score. Perceived fitness was significantly different than actual fitness (F (1,100) = 31.3, {dollar}p <{dollar} 0.001) and there was a significant gender difference (F (1,100) = 13.56, {dollar}p <{dollar} 0.001). However, a significant interaction (F (1,100) = 21.88, {dollar}p <{dollar} 0.0001) indicated that the difference between perceived and actual fitness was not consistent for males and females. Further analysis revealed that females were able to estimate their fitness level more accurately than males. The results indicate that perceived fitness is a valid indicator of actual fitness for women but not for men.

Comparison Of Laryngographic Waveforms Of An Adult And Child, Elaine Hicken

Undergraduate Honors Capstone Projects

The ability to accurately identify and differentially diagnose voice disorders is an important concern for the speech-language pathologist. This need has resulted in the development of methods which focus on identifying "objective, reliable, valid procedures for assessing voice disorders" (Costello, 1985). Several methods that allow direct observation of the laryngeal mechanism are invasive and time consuming. A method that is noninvasive and that provides valuable information about the vocal fold movement pattern is needed for clinical use.

Depressive Deficits In Memory: Focusing Attention Improves Subsequent Recall, Paula T. Hertel, S. S. Rude

Psychology Faculty Research

58 Ss (aged up to 55 yrs) diagnosed as depressed, recovered from depression, or without a history of depression performed an unintentional learning task, followed by tests of free and forced recall. In the learning task, Ss decided whether a series of nouns sensibly completed corresponding sentence frames that varied in decision difficulty. For half of the Ss, the focus of attention was unconstrained by the demands of this task. The others, however, were required to repeat the targeted noun at the end of the trial as a means of focusing their attention on the task. Depressed Ss in the …

Home Adaptations For Persons With Chronic Disabilities: An Educational Model., Ruth E. Levine, Laura N. Giltin

Department of Occupational Therapy Faculty Papers

This paper offers a method by which to train students in the provision of culturally relevant, in-home environmental adaptations for persons with chronic disabilities. On the basis of a theoretical framework, the student therapist learns about the client's life-style and offers adaptations that evolve from a collaborative problem-solving process. The training process has been developed and refined over a 3-year period. A case study illustrates the student's application of theory and practice and the outcome for the client of this service provision approach.

Osteogenesis Imperfecta. The Position Of Substitution For Glycine By Cysteine In The Triple Helical Domain Of The Pro Alpha 1(I) Chains Of Type I Collagen Determines The Clinical Phenotype., Barbra J. Starman, David Eyre, Harry Charbonneau, Maria Harrylock, Mary Ann Weis, Lester Weiss, John M. Graham Jr., Peter H. Byers

Dartmouth Scholarship

Skin fibroblasts grown from three individuals with osteogenesis imperfecta (OI) each synthesized a population of normal type I collagen molecules and additional molecules that had one or two alpha 1(I) chains that contained a cysteine residue within the triple-helical domain, a region from which cysteine normally is excluded. The patients had very different phenotypes. One patient with OI type I had a population of alpha 1(I) chains in which glycine at position 94 of the triple helix was substituted by cysteine; a patient with OI type III had a population of alpha 1(I) chains in which glycine at position 526 …

Maternal-Neonatal Serum Vitamin A Concentrations., Girish S. Shirali, D G. Oelberg, K P. Mehta

Manuscripts, Articles, Book Chapters and Other Papers

Prevention of neonatal vitamin A deficiency is related to the adequacy of maternal vitamin A stores. In this study we investigated maternal and cord serum vitamin A and retinol-binding protein (RBP) values in an Indian population including, for the first time, clinically vitamin A-deficient mothers. Twenty-eight maternal-neonatal pairs were selected from maternal cohorts of high socioeconomic status without clinical evidence of vitamin A deficiency (group I) and low socioeconomic status with conjunctival xerosis and Bitot's spots (group II). Maternal education, caloric and vitamin A intakes, weight, height, hemoglobin, and birth weight were significantly lower in group II. Serum vitamin A …

Oral Lichenoid Reactions During Antimalarial Prophylaxis With Sulphadoxine-Pyrimethamine Combination, R.B. Zain

Pre-2000

The prevalence of clinically observed oral lichenoid reaction in 186 Malay army personnel using Fansidar for 9 weeks was found to be 4.8. The prevalence was found to be 0.5 in 186 army personnel who had stopped using Fansidar for 2 months and 0 in 143 army personnel (control group) who had not used Fansidar for at least 4 months. The lesion showed a higher prevalence for the gingiva. There was no correlation between cigarette smoking and the occurrence of these lesions in each group

The Social Impact Of Oral And Facial Pain In An Industrial Population, N. Jaafar, I.A. Razak, R.B. Zain

Pre-2000

The aim of this study is to determine the social impact of oral and facial pain in a sample involving an industrial population. Out of a total of 355 subjects interviewed, nearly one-half claimed to have oral and facial pain in the previous one month prior to the survey. The most common type of pain was that related to hot or cold fluids or sweet things followed by toothache. On the average, the pain lasted for 4.2 days (SD = 4.9) per person in the past one-month. About one in five persons with pain reported that it was severe enough …

Adult Age Differences In Knowledge Of Retrieval Processes, L. J. Anooshian, S. L. Mammarella, Paula T. Hertel

Psychology Faculty Research

We assessed knowledge of retrieval processes in young (25-35 years) and old adults (70-85 years). Both feeling-of-knowing judgments and retrieval monitoring were examined with a set of questions about recent news events. For answers that participants initially failed to recall, they rated their feeling-of-knowing as well as made predictions regarding the likelihood of recalling the answer with the aid of a specified type of retrieval cue (retrieval monitoring). Accuracy was evaluated in the context of later recall or recognition performance. We found age group differences in the accuracy of retrieval monitoring, free recall, and recall aided by phonological cues. Using …

The Reliability Of Alcohol Abusers’ Self-Reports Of Drinking And Life Events That Occurred In The Distant Past, Linda C. Sobell, Mark B. Sobell, Diane M. Riley, Reinhard Schuller, D. Sigfrido Pavan, Anthony Cancilla, Felix Klajner, Gloria I. Leo

Faculty Articles

This study investigated the test-retest reliability of 69 alcohol abusers' current reports about their past (approximately 8 years prior to interview) drinking behavior and life events. Drinking behavior was assessed by the Lifetime Drinking History (LDH) questionnaire and life events were assessed using the Recent Life Changes Questionnaire (RLCQ). Reliability coefficients for LDH variables were generally moderate to high (r = .52 to .81). Using empirical criteria, the diagnostic power of the two LDH interviews to classify correctly subjects as either having had or not having had a drinking problem was quite high. The reliability coefficient for the RLCQ was …

The Dental Health Of Factory Workers In Pasir Gudang, Johor (Malaysia), Z. Abdul Majid, R.B. Zain

Pre-2000

A study on the oral health status of 198 factory workers (in Antara Steel, Pasir Gudang, Johor) was carried out in November 1986. The subjects were predominantly male (94%) and Malay (91%) with an average age of 27.4 years. The prevalence of caries was 87.4% with a mean DMFT of 7.1 ? 5.4. There were 3 subjects with full upper, lower dentures and 16 subjects with partial dentures. Two subjects examined needed dentures but were not wearing them. Thirty five (17.7%) of those examined needed urgent extractions, and 8 needed emergency treatment for fillings. Enamel opacities were found in 141 …

Community-Based Occupational Therapy With A Head-Injured Adult., Elizabeth Depoy

Department of Occupational Therapy Faculty Papers

In the early 1970s, the National Head Injury Foundation identified 422,000 adults with permanent brain damage caused by traumatic head injury. It is estimated that 400,000 new cases of varying severity are treated in hospitals each year, the majority of whom are previously employed young adult men. Although many persons with traumatic brain injuries are able to return to productivity, approximately 35% of the adults who have been rated as mildly head injured on the Glascow Coma Scale (Teasdale & Jennet, 1974) never return to work and have difficulty reentering society after restorative efforts are discontinued (Rosenthal, Griffith, Bond, & …

Haemophilus Influenzae In Adults: An Analysis Of Incidence, Serotype, Biotype, Beta-Lactamase Production And Clinical Spectrum, Mildred Ramsey Norris

MSN Research Projects

A selective medium, chocolate agar with vancomycin, bacitracin, and clindamycin, was used to enhance the recovery of Haemophilus influenzae from adult patients. Cultures of the 634 respiratory specimens yielded 130 (20.5 percent) H. influenzae isolates, representing 93 patients. The serotype, biotype, and Beta-lactamase production of all isolates were determined. Of 103 distinct isolates, nine percent were serotypable and included serogroups a ,b ,d ,and e. Although biotypes I, II, III, V, and VI were represented, 81.5 percent of the isolates were in the II/III group. Twenty-six percent of the isolates were Beta-lactamase producers and, therefore, ampicillin resistant. The clinical characteristics …

Heterogeneity Of Clonogenic Cells In Acute Myeloblastic Leukemia., Kert D. Sabbath, Edward D. Ball, Peter Larcom, Roger B. Davis, James D. Griffin

Dartmouth Scholarship

The expression of differentiation-associated surface antigens by the clonogenic leukemic cells from 20 patients with acute myeloblastic leukemia (AML) was studied with a panel of seven cytotoxic monoclonal antibodies (anti-Ia, -MY9, -PM-81, -AML-2-23, -Mol, -Mo2, and -MY3). The surface antigen phenotypes of the clonogenic cells were compared with the phenotypes of the whole leukemic cell population, and with the phenotypes of normal hematopoietic progenitor cells. In each case the clonogenic leukemic cells were found within a distinct subpopulation that was less "differentiated" than the total cell population. Clonogenic leukemic cells from different patients could be divided into three phenotype groups. …

Genetics And Epidemiology Of Gallbladder Disease In New World Native Peoples, K M Weiss, R E Ferrell, C L Hanis, P N Styne

Faculty, Staff and Student Publications

Native peoples of the New World, including Amerindians and admixed Latin Americans such as Mexican-Americans, are highly susceptible to diseases of the gallbladder. These include cholesterol cholelithiasis (gallstones) and its complications, as well as cancer of the gallbladder. Although there is clearly some necessary dietary or other environmental risk factor involved, the pattern of disease prevalence is geographically associated with the distribution of genes of aboriginal Amerindian origin, and levels of risk generally correspond to the degree of Amerindian admixture. This pattern differs from that generally associated with Westernization, which suggests a gene-environment interaction, and that within an admixed population …

Criterion Intervals For Pretreatment Drinking Measures In Treatment Evaluation, A. Mitch Cooper, Mark B. Sobell, Stephen A. Maisto, Linda C. Sobell

Faculty Articles

Drinking during a 30-day pretreatment period was found not to be representative of longer pretreatment intervals, especially in a population of seriously impaired inpatient alcoholics