Digital Commons Network^™

Inducible Pou Class 2 Homeobox 3 Expression In Human Intestinal Organoids As A Model Of Human Tuft Cells, Carolyn Bomidi, Luqiong Wang, Hoa Nguyen-Phuc, Mary K Estes, Sarah E Blutt

Faculty, Staff and Students Publications

No abstract provided.

Sewers To Solutions: A Guide To Wastewater Pathogen Monitoring, Justin R Clark, Anthony W Maresso

Faculty, Staff and Students Publications

Wastewater-based epidemiology (WBE) is the analysis of wastewater to detect pathogen levels or activity for public health awareness or action. Pioneered in the 1940s, WBE underwent a resurgence during the COVID-19 pandemic, providing important information about number of cases, outbreaks, and seasonal impact. With advancements in detection technologies and growing interest in environmental surveillance, WBE is poised to become a standard practice in public health monitoring. Here, we provide an overview of the current state of the art of pathogen WBE, including methods of molecular detection, analysis of wastewater data, real-world applications and programs, public health interventions, and benefits and …

Editorial: Advances In Phage Applications: Deciphering Phage Biological And Ecological Mechanisms Through Metagenomics, Tasha M Santiago-Rodriguez, Gary A Toranzos

Faculty, Staff and Students Publications

No abstract provided.

Identification Of Cand1 As A Dna-Dependent Protein Kinase-Regulated Coactivator Of Androgen Receptor And The Arv7 Splice Variant, Ross A Hamilton, Basil Paul, Ping Yi, Kimal Rajapakshe, Anil K Panigrahi, Sandra L Grimm, Cristian Coarfa, Anna Malovannaya, Nancy L Weigel, David M Lonard, Charles E Foulds

Faculty, Staff and Students Publications

ARv7, the most prevalent androgen receptor (AR) variant in castration-resistant prostate cancer, lacks the ligand binding domain (LBD), rendering it resistant to LBD-targeted therapies. Identifying new therapeutic targets requires defining the coregulators and associated regulatory enzymes that govern AR and ARv7 transcriptional activity. Here, we have developed a cell-free DNA pulldown assay employing androgen response elements (AREs) to isolate and characterize the AR- and ARv7-associated coregulator complexes formed on DNA. Mass spectrometry analyses of ARE DNA pulldowns revealed previously unrecognized AR and ARv7 associating coregulators, such as cullin-associated NEDD8-dissociated protein 1 (CAND1), in addition to previously known coregulators. ARv7 showed …

Genomic And Structural Analysis Of Major Antigenic Proteins’ Variability In The Human Relapsing Fever Spirochete Borrelia Persica, Dor Shwartz, Yaarit Nachum-Biala, Reinhard K Straubinger, Ofir Segev, Asaf Levy, Job E Lopez, Gad Baneth

Faculty, Staff and Students Publications

Antigenic variation enables immune evasion of pathogens, yet the mechanisms for the rapid change in their antigens without loss of structural and functional features are not fully known. Tick-borne relapsing fever (TBRF) spirochetes of the genus Borrelia can be used as a model because their variable major proteins (Vmps) drive antigenic variation followed by recurrent waves of bacteremia. In this study we generated the genome of Borrelia persica LMU-C01 and evaluated Vmp structure across relapsing fever Borrelia species. Borrelia persica was found to have a genome of 1.7Mb with one linear chromosome, 12 linear and one circular plasmids. Telomeric hairpin …

Hybrid Sars-Cov-2 Immunity Among Frontline Workers In A High-Tourism Setting: A Community-Based Serosurvey In Ambergris Caye, Belize, June 2022, Oluwadara Okeremi, Ella Hawes, Anh N Ly, Russell Manzanero, Sarah M Gunter, Shannon E Ronca, Allison Lino, Allyson Hidalgo, Meghan Si, Allison Stewart-Ruano, Adriana Maliga, Eric J Nilles, Gerhaldine Morazan, Kristy O Murray

Faculty, Staff and Students Publications

Tourism is central to Belize's economy, yet the influx of international travelers may contribute to infectious disease introduction and transmission among local workers, including SARS-CoV-2. In June 2022, we conducted a cross-sectional seroepidemiologic study among tourism and government workers in the tropical island tourism center of Ambergris Caye, Belize. The goal of this study was to determine SARS-CoV-2 antibody prevalence for both vaccination (anti-spike protein) and natural infection (anti-nucleocapsid protein), vaccine uptake, history of COVID-like symptoms, and risks for infection two years into the pandemic. Workers from 30 hotels and government agencies were enrolled. Participants completed questionnaires to capture demographics, …

Autologous Multiantigen-Targeted T Cell Therapy For Pancreatic Cancer: A Phase 1/2 Trial, Benjamin L Musher, Spyridoula Vasileiou, Brandon G Smaglo, Catherine S Robertson, Mengfen Wu, Tao Wang, Ayumi Watanabe, Manik Kuvalekar, Yovana Velazquez, Shamika Ketkar, Tamadar Al Doheyan, Penelope G Papayanni, Aakash Shah, Natalia Lapteva, Bambi J Grilley, George Van Buren, Premal D Lulla, Helen E Heslop, Cliona M Rooney, Malcolm K Brenner, Ann M Leen

Faculty, Staff and Students Publications

T cell therapy has proven challenging for pancreatic ductal adenocarcinoma (PDAC), partly due to heterogeneous expression of tumor-associated antigens (TAAs). To address tumor heterogeneity and mitigate immune evasion, an ex vivo expanded, polyclonal, T helper 1 cell-polarized T cell product targeting five TAAs—PRAME, SSX2, MAGEA4, Survivin and NY-ESO-1—was developed. These antigens were chosen based on their tumor specificity, oncogenicity, immunogenicity and level of expression. In a phase 1/2 trial, this autologous nonengineered T cell product was administered (1 × 107 cells m−2 per infusion) monthly to patients with advanced PDAC responding (arm A, n = 13) or refractory (arm B, …

Discovery Of Genetic Susceptibility Variants In Pediatric And Adult Ependymoma, Joshua D Strauss, Priya B Shetty, Spiridon Tsavachidis, Jinyoung Byun, Stephen C Mack, Xiao Xiangjun, Terri S Armstrong, Mark R Gilbert, (On Behalf Of Nci-Connect);, Lisa Mirabello, Smita Bhatia, Wendy M Leisenring, Lindsay M Morton, Gregory T Armstrong, Jon Foss-Skiftesvik, Christian Munch Hagen, Jonas Bybjerg-Grauholm, (On Behalf Of Gicc), Manel Ghozal, Audrey Bonaventure, Jacqueline Clavel, Melissa L Bondy, Christopher I Amos, Thanh T Hoang, Michael E Scheurer

Faculty, Staff and Students Publications

Background: Ependymoma is a malignancy of the neuroepithelium-derived ependyma that lines the spinal cord and ventricles of the brain, occurring most frequently in young children and older adults. Genetic susceptibility to ependymoma has proven difficult to assess due to disease rarity.

Methods: We performed genome-wide association studies (GWAS) of 478 ependymoma patients and 4,841 disease-free controls of European ancestry. Ependymoma patients consisted of 117 children (< 18 years old) with whole-genome sequencing (WGS), 142 children with genotyping, and 219 adults (≥18 years old) with genotyping. Genotyped samples were imputed using the 1,000 Genomes Project as the reference panel and underwent quality control filtering. The GWAS was performed separately by age group and technology (genotyped or WGS). GWAS variants were considered significant at P < 5 × 10-8.

Results: Among pediatric subjects with WGS data, we identified a significant intronic variant in EDIL3 (rs149378, P = 1.9 × 10-8) and a nearly significant intronic variant in LHX4 (rs79008224, P = 7.2 …

Small-World Simulations: A New Method To Test The Effect Of Legal Rules, Johannes Kruse

Coase-Sandor Institute for Law & Economics Research Paper Series

Small-world simulation exposes an artificial society to a legal rule and observes what its members do. The agents are driven by language models: their conduct is not stipulated in equations but reasoned in their own words. The architecture can be turned on any legal regime; personalized speed limits are the demonstration, chosen because rational-choice and behavioral predictions there conflict. Validated against documented driving patterns, the simulation generates behavior no one wrote into it. Because language models are trained on what people say rather than do, the method estimates orderings, not levels: a benchmark, not a substitute for evidence.

A Qualitative Study Of Undergraduate Underrepresented Minoritized Students’ Online Learning Experiences, Susanne Morris

Theses and Dissertations--Curriculum and Instruction

This dissertation reports a qualitative secondary analysis of 37 semi-structured interviews with 40 undergraduate underrepresented minoritized (URM) students. Guided by social constructionism and intersectionality, the study explores how students make sense of online learning as a sociomaterial environment, attending to perceptions of constraints, affordances, and missing or misaligned signifiers. Interviews were analyzed using Braun and Clarke’s Reflexive Thematic Analysis to examine how institutional norms and course design shape what students experience as doable, safe, and meaningful. The study offers insight relevant to research and practice in equity and online learning.

Adolescent Relationships With Digital Actors And Their Influence In A Classroom Setting: An Interpretive Case Study, Kerry A. Hancock

Theses and Dissertations--Curriculum and Instruction

In this post-COVID-19 society students are strongly influenced by their use of digital text and electronic devices. The assumption of the study was that if students spend time with devices and digital text that it might be possible for them to develop relationships with the non-human actors that exist as part of the learning environments in schools. This  interpretive case study rooted, in Posthumanist ideology, used Latour’s Actor Network Theory to approach data collection.

A comparative approach was adopted for this interpretive case study research and data were collected in three stages: initial classroom observation and analysis to determine the …

Industry To Classroom: The Role Of Academic Program Consultants In Building Self-Efficacy Among Occupation-Based Career And Technical Education Teachers, Erica L. Settelen

Theses and Dissertations--Educational Leadership Studies

Occupation-based career and technical education (CTE) teachers bring industry expertise to secondary classrooms but often begin teaching without traditional pedagogical preparation or familiarity with public school systems. In Kentucky’s New Teacher Institute (NTI), academic program consultants (APCs) provide program-area support to strengthen induction. Using the framework of self-efficacy and guided by Ivankova’s mixed methods action research (MMAR) framework, this study examined how occupation-based CTE teachers perceived APC engagement during induction and how APC-created asynchronous supports were experienced.

In the Reconnaissance Phase, a sequential Quantitative → Qualitative design included an initial survey followed by interviews. Survey results indicated midrange familiarity with …

Responding To The Needs Of First-Generation Students: An Action Research Study Of Professional Development Opportunities For Faculty And Staff, Kyle G. Phillips

Theses and Dissertations--Educational Leadership Studies

First-generation college students have historically been considered marginalized in institutions of higher education, with diminished success rates and disproportionately higher incidents of imposter syndrome. This Mixed Methods Action Research study assessed the extent to which faculty and staff are able to recognize and implement research-based best practices to improve the support system surrounding this student demographic. In particular, it assessed current levels of preparation to support first-generation students and sought to identify and implement an appropriate professional development program as part of the study's intervention. The efficacy of the intervention was then assessed, with the result delineated in this dissertation. …

From Trauma To Triumph: The Relationship Between Psychological Resilience And Academic Resilience In Graduate School Students With Adverse Childhood Experiences, Jade Gingue

Theses and Dissertations--Educational Leadership Studies

This dissertation investigated the relationship between adverse childhood experiences (ACEs), psychological resilience, and academic resilience among graduate students. Grounded in resilience theory and interpreted through a trauma-informed, strengths-based lens, the study examined whether psychological resilience functions as a buffering moderator or as a direct protective factor among graduate students.

Using a quantitative design, data were collected via Qualtrics from graduate students (n = 391). This study used three previously validated instruments, including the Adverse Childhood Experiences Questionnaire (ACE-10), the Connor-Davidson Resilience Scale (CD-RISC-25), and the Academic Resilience Scale (ARS-6), along with demographic measures. Hierarchical multiple regression analyses were conducted …

Recruitment Messaging, Authenticity, And Representation: A Mixed Methods Action Research Study In A Public School System, Chavis A. Sims

Theses and Dissertations--Educational Leadership Studies

This study employed a Mixed Methods Action Research (MMAR) design to examine recruitment communication within a public school system facing persistent underrepresentation of minority educators.  Recruitment prototypes were developed in collaboration with district leadership and evaluated in a formative pilot involving 15 educators who provided Likert-scale ratings and written feedback on clarity, cultural relevance, persuasiveness, and engagement.  Quantitative findings indicated that messaging emphasizing professional purpose and pathways into teaching was perceived as clear and engaging.  In contrast, cultural relevance consistently received the lowest ratings across prototypes.  Qualitative responses revealed that reliance on non-local and AI-generated imagery weakened perceived authenticity and …

The Development Of Self-Efficacy Among New Residence Life Professionals, Kenyatta S. Jeter

Theses and Dissertations--Educational Leadership Studies

New professionals in student affairs, particularly those working in housing and residence life, face complex job responsibilities that demand a high level of confidence and preparation. The purpose of this study was to increase resident directors’ sense of self-efficacy as they transition into their new role. This study employs a mixed methods action research (MMAR) design to explore the development of an effective onboarding and training framework to support housing and residence life professionals in successfully transitioning new resident directors into their roles.

An intervention plan was developed in collaboration with key stakeholders to increase resident directors’ sense of self-efficacy …

Problem-Based And Practice-Driven: Designing For Engagement In An Online Educational Leadership Program, Owens G. Saylor

Theses and Dissertations--Educational Leadership Studies

Virtual professional learning has emerged as a flexible and scalable solution for developing educational leaders, particularly those navigating demanding roles and geographic barriers. However, these benefits often come at the cost of diminished engagement, interaction, and practical application to real-world leadership challenges. Without intentional design, virtual sessions risk becoming passive and disconnected, undermining their professional development value. This study responds to these challenges by exploring how a problem-based learning (PBL) approach can improve engagement in the virtual component of the Kentucky Leadership Rising (KLR) Aspiring Superintendent Workshop Series.

This mixed methods action research (MMAR) study examined participants’ perceptions of engagement …

Enhancing International School Implementation Capacity: Supporting Contextualized Learning At An International Baccalaureate School In China, Christopher J. Hayden Iv

Theses and Dissertations--Educational Leadership Studies

International schools allocate substantial resources to implement progressive curricular innovations; however, the advantages offered by these developments are contingent on their effective execution within classroom settings. This mixed methods action research (MMAR) study examined the implementation of the International Baccalaureate (IB) Global Context (GC) Strategy in the Middle Years Programme (MYP) at an international school in China and asked how the school might strengthen its capacity to enact this and similar innovations. Following a sequential MMAR design, a reconnaissance phase combined a whole-staff survey (n=87) with qualitative interviews to diagnose the individual, organizational, and contextual factors shaping implementation. Analyses identified …

Perceptions Of Sport Psychology Efforts And Access In Women's Wheelchair Basketball, Lindsay C. Cohen

Theses and Dissertations--Kinesiology and Health Promotion

As the field of sport psychology grows, services and training programs must evolve to properly serve the evolution of athletes seeking care. This could mean discovering new measures, testing different types of interventions, or developing programs that can better accommodate specific populations that are looking for sport psychology services. One such population is para athletes, and although they have expressed interest in learning more about the field of sport psychology and how to implement mental skills efficiently, athletes have reported a lack of access to sport psychology services and sport psychology consultants (SPCs) (Dieffenbach et al., 2009). The purpose of …

Unveiling The Shadows: An Exploratory Study On Ghostwriting, Gray Markets, And The Ethics Of Contract Cheating, Micah Crawford

Theses and Dissertations--Educational Policy Studies and Evaluation

Contract cheating has become an increasingly complex challenge for higher education, particularly as digital platforms, global academic labor markets, and generative artificial intelligence reshape the production and detection of academic work. While much of the existing literature examines contract cheating from the perspectives of students, faculty, and institutions, less attention has been given to the individuals who produce ghostwritten academic work. This dissertation addresses that gap by exploring how shadow scholars understand their roles, ethical boundaries, and participation within the academic integrity ecosystem.

Using an exploratory qualitative design, this study draws on twelve semi-structured narrative interviews with six freelance academic …

Biomechanical Effects Of Exercise-Based Interventions In Chronic Nonspecific Low Back Pain: A Systematic Review And Meta-Analysis, Lara De Vecchi Machado

Theses and Dissertations--Kinesiology and Health Promotion

Chronic nonspecific low back pain (CNLBP) is the leading cause of years lived with disability worldwide and represents a complex, multifactorial, and heterogeneous condition. It is characterized by substantial interindividual variability in muscle activation patterns, lumbo-pelvic coordination, and movement strategies. Although exercise is widely recommended as a first-line intervention and its clinical benefits are well established, the biomechanical mechanisms underlying functional improvement remain fragmented in the literature and are rarely synthesized quantitatively.

This dissertation conducted a systematic review with meta-analysis to investigate the biomechanical effects of exercise-based interventions in individuals with CNLBP. A total of 39 studies were included and …

Test-Retest Reliability And Validity Of The Gas Exchange Threshold, Erik Ryan Snell Mr.

Theses and Dissertations--Kinesiology and Health Promotion

Theoretically, the gas exchange threshold (GET) demarcates the moderate and heavy intensity domains, whereby exercise performed at and below the GET results in a change in oxygen consumption ([ΔVO₂] ≤ 200 mL), and exercise above the GET results in a slow component (ΔVO₂ > 200 mL). This study examined the reliability and validity of the GET. 14 subjects performed two maximal graded exercise tests (GXTs) to determine the GET and its reliability. The minimal difference (MD = 21W) was applied to determine the GET above (GET_+MD) and below (GET_-MD) power outputs for 30 min …

Are Multiple-Choice And Open-Ended Assessments Consistent In Predicting Science Achievement? Multivariate Multilevel Evidence From Chinese Students And Schools, Quan Wu

Theses and Dissertations--Education Sciences

A multivariate multilevel model was developed and applied to secondary data from a large-scale science assessment of Grade 8 students in China to examine the consistency between multiple-choice and open-ended formats of assessment in predicting science achievement at the student and school levels. The sample included 44,534 students from 159 schools across two regions. Multivariate multilevel results indicated a moderately positive consistency between the two formats at the student level and a strongly positive consistency at the school level; these consistencies were stable across regions and did not depend on student and school characteristics. The findings have important practical implications …

Effects Of Yoga And Mindfulness On Emotional Regulation, Behavior, And Teacher Burnout, Sara E. Smith

Theses and Dissertations--Education Sciences

This dissertation comprises three studies that examine the effects of physical activity, yoga, and mindfulness interventions on students' emotional regulation, behavior, and academic outcomes in rural Kentucky schools. These studies as a whole contribute to understanding how movement-based and mindfulness practices can enhance social-emotional and academic well-being in schools.

The first study describes the effects of classroom-based physical activity on student achievement and social-emotional well-being. Forty-five elementary students participated in a 6-week intervention with two 7-minute movement breaks per day. Results indicated statistically significant improvements in Reading (t(44) = 2.55, p < .001, d = .38), Math (t(44) = 4.63, p < .001, d = .69), and social-emotional competence (t(44) = 3.67, p < .001, d = .55). These findings demonstrate that brief, structured physical activity can meaningfully enhance academic and emotional outcomes when implemented by classroom teachers.

The second study shows the effects of a five-week …

Leadership Perceptions In Mid-Major Ncaa Athletics: Exploring Women's Teams Head Coaches' Experiences With Athletic Department Senior Staff, Sydnie A. Steele

Theses and Dissertations--Education Sciences

Leadership practices within NCAA Division I athletic departments can shape the professional climate, well-being, and stability of the coaching staff. Yet, limited research has explored how senior administrators influence the experiences of coaches who lead women’s teams at mid-major institutions. This phenomenological inquiry study explored how head coaches interpret their interactions with senior athletic department staff and how these relational dynamics inform perceptions of support.

Twelve Division I head coaches participated in semi-structured Zoom interviews about their experiences at former institutions. The interview protocol integrated the LMX-7 framework with open-ended questions to capture the significance of the head coaches’ lived …

Exploring High School Students’ Learning Interests And Information Sources On Viruses And Covid-19, Sahar Alameh, James W. Keck, Elizabeth Loh, Lin Xiang, Scott M. Berry, Anna G. Hoover, Abigail Knapp, Savannah Tucker

Science, Technology, Engineering, and Mathematics (STEM) Education Faculty Publications

This study investigates high school students’ health-information seeking behaviors related to COVID-19 and viruses in a rural area of the south-central United States, and how these behaviors relate to students’ knowledge, perceptions, and learning interests. Survey data were collected from 83 students in Spring 2023, during the post-pandemic period, as part of a STEM and public health unit. Drawing on frameworks from Health Information-Seeking Behavior and student interest, the study examines students’ COVID-19 information sources, how these sources relate to their knowledge about viruses and their perceptions of COVID-19, and the specific topics about which they were most interested in …

Exploring The Relationships Between Social Influence, Vaping Behaviors, And Susceptibility Across Grade Levels In Kentucky, Raphael Adesiyan, Odunayo O. Omotilewa, Saber Feizy, Melinda J. Ickes

Kinesiology and Health Promotion Faculty Publications

Purpose: This study explored the relationships between social influence, vaping behavior, and susceptibility across different grade levels.   Design: The study design was cross-sectional. Data were collected from middle and high school students who voluntarily participated between September 2022 and April 2023.   Sample: A convenience sample of students in 6^th to 12^th grades in Kentucky (N = 4677).   Measures: Demographic information, including age, grade level, school location, and race/ethnicity, was collected. The outcome variables included social influence, susceptibility, and vaping behavior.   Analysis: Logistic regression analysis was used to explore the relationships between social influence, vaping behavior, and susceptibility.   Results: …

From Mandate To Meaning: Designing Implementation Supports For Organizational Readiness In Behavioral Health Service Planning, Lauren K. Mergen

Theses and Dissertations--Education Sciences

Behavioral health agencies often struggle to translate the Transformational Collaborative Outcomes Management (TCOM) framework into practice when implementation is externally mandated. This study examined how educational leaders can support meaningful use of TCOM tools under a Midwestern state’s October 2024 requirement that behavioral health providers use the state Child and Adolescent Needs and Strengths assessment in service planning.

Using a Mixed Methods Action Research design, the study progressed through six phases: Diagnosis, Reconnaissance, Planning, Acting, Evaluation, and Monitoring. The Diagnosis Phase identified a knowledge-to-practice gap and weaknesses in implementation support infrastructure. Practitioner surveys administered immediately after training and six months …

Bridging The Intention–Behavior Gap In Mental Health Help-Seeking, Wenqi Weng

Theses and Dissertations--Educational, School, and Counseling Psychology

Objective: To examine whether modifiable factors shape the extent to which help-seeking intention is translated into formal mental health service initiation among distressed adults. Method: Participants were U.S. adults (N = 777; M age = 47.21 years, SD = 16.70; 73.23% women; 77.74% White) who reported at least moderate psychological distress and were not currently receiving formal mental health treatment. At baseline, participants completed measures of help-seeking intention, focal moderators (action planning, psychological distress, and mental health steward self-identity), coping planning, cues to action, and emotion regulation as exploratory candidate moderators. At three-month follow-up, participants reported whether …

Academic Departments As Microfoundations Of Institutional Governance, Karley A. Riffe, Meghan J. Pifer

Educational Policy Studies and Evaluation Faculty Publications

Considerations of governance in US higher education do not typically include the academic department. This results in a knowledge gap about the role of these central organizing units of academic work in the governance of colleges and universities. Through the concept of microfoundations, we explore concrete ways in which departmental governance matters, how it may be challenging, and strategies for fostering effective governance within academic departments.