Digital Commons Network^™

Engineering Mutation Clones In Mammalian Cells With Crispr/Cas9, Zijun Huo, Jian Tu, Rachel Shoemaker, Dung-Fang Lee, Ruiying Zhao

Faculty, Staff and Student Publications

CRISPR, Clustered Regularly Interspaced Short Palindromic Repeat, as a powerful genome engineering system, has been widely accepted and employed in gene editing of a vast range of cell types. Compared to zinc finger nucleases (ZFNs) or transcription activator-like effector nucleases (TALENs), CRISPR shows a less complicated process and higher efficiency. With the development of different CRISPR systems, it can be used not only to knock out a gene but also to make precise modifications, activate or repress target genes with epigenetic modifications, and even for genome wide screening. Here we will describe the procedure of generating a stable cell line …

Host Modulation Therapy In Periodontitis: From Established Therapies To Emerging Technologies, Aonjittra Phanrungsuwan, Jiaqi Huang, Neeraja Dharmaraj, Alejandra Cobos Perez, Omid Veiseh, Simon Young, Chun-Teh Lee

Faculty, Staff and Student Publications

Periodontitis is a biofilm-induced chronic inflammatory disease, characterized by gingival inflammation and alveolar bone loss. According to a national survey, approximately half of the U.S. adults are affected by periodontal disease. To effectively prevent and treat periodontitis, it is essential to address its underlying causes. The primary etiological factors include polymicrobial synergy and dysbiosis of the oral microbiota, and a dysregulated immune response. The standard therapeutic approach, mechanical removal of biofilm through debridement, sometimes demonstrates limited efficacy, particularly in cases of severe periodontitis, which may require adjunctive or additional therapy. Emerging evidence indicates that periodontal tissue destruction is initiated by …

Grading Melanocytic Dysplasia: Updated Histopathologic Criteria, Christopher R Shea, Victor G Prieto, Catherine M Shachaf, Scott R Florell

Faculty, Staff and Student Publications

Dr. Martin C. Mihm, Jr.'s innovative work on the dysplastic nevus achieved a milestone in his chapter in the World Health Organisation Classification of Skin Tumours (WHO-C). WHO-C presents a dichotomous classification (high-grade versus low-grade dysplastic nevi) and a quantitative metric to assess melanocytic nuclear enlargement. The Duke classification is a related approach that provides mostly quantitative histopathologic criteria for dysplastic nevi and gives due weight to architectural features as well as cytology. This paper proposes and illustrates updated criteria for scoring and grading melanocytic dysplasia, incorporating some of the definitions and categories of WHO-C, while refining the quantitative and …

Nsd2 Inhibitors Rewire Chromatin To Treat Lung And Pancreatic Cancers, Jinho Jeong, Simone Hausmann, Hanyang Dong, Kacper Szczepski, Natasha M Flores, Andy Garcia Gonzalez, Liyang Shi, Xiaoyin Lu, Joanna Lempiäinen, Moritz Jakab, Liyong Zeng, Tourkian Chasan, Eric Bareke, Rui Dong, Emma Carlson, Reinnier Padilla, Dylan Husmann, Julia Thompson, Gerry A Shipman, Emily Zahn, Courtney A Barnes, Laiba F Khan, Liz Marie Albertorio-Sáez, Eva Brill, Vishnu Udayakumar Sunita Kumary, Matthew R Marunde, Danielle N Maryanski, Cheryl C Szany, Bryan J Venters, Carolina Lin Windham, Michal Eligiusz Nowakowski, Iwona Czaban, Mariusz Jaremko, Michael-Christopher Keogh, Kang Le, Michael J Soth, Benjamin A Garcia, Łukasz Jaremko, Jacek Majewski, Pawel K Mazur, Or Gozani

Faculty, Staff and Student Publications

NSD2 catalyses the epigenetic modification H3K36me2 (refs. 1,2) and is a candidate convergent downstream effector of oncogenic signalling in diverse malignancies3–5. However, it remains unclear whether the enzymatic activity of NSD2 is therapeutically targetable. Here we characterize a series of clinical-grade small-molecule catalytic NSD2 inhibitors (NSD2i) and show that the pharmacological targeting of NSD2 constitutes an epigenetic dependency with broad therapeutic efficacy in KRAS-driven preclinical cancer models. NSD2i inhibits NSD2 with single-digit nanomolar half-maximal inhibitory concentration potency and high selectivity over related methyltransferases. Structural analyses reveal that the specificity of NSD2i for NSD2 …

Evolving Molecular Subtypes Of Gastric Cancer: From Past Classifications To Present Consensus And Future Directions For Precision Therapy, Ju-Seog Lee

Faculty, Staff and Student Publications

Gastric cancer is a biologically heterogeneous disease. The advent of high-throughput multi-omic technologies has revolutionized our understanding of gastric cancer by deconstructing this heterogeneous entity into distinct and more homogeneous molecular subtypes. Early classifications based on gene expression, methylation, and histology have laid the groundwork for multi-omic frameworks proposed by The Cancer Genome Atlas and Asian Cancer Research Group, which established the foundation of modern molecular taxonomy. Subsequent integrative efforts, particularly the Consensus Genomic Subtypes (Super 6) model, have unified this collected information into clinically relevant subtypes that bridge prognostic stratification with treatment strategies. Established biomarkers such as human epidermal …

Prickle4 Drives Microenvironmental Remodeling And Resistance To Parp Inhibition In Idh-Mutant Glioma, Ju Yang, Hua Yang, Yifan Yuan, Chenyang Zhang, Ziwei Fu, Yanyan Chen, Yinghong Xiong, Shuyu Chen, Kexin Ling, Ying Liu, Jason T Huse, Bo Chen, Timothy A Chan, Zengxin Qi, Zhao Zhang, Xiuping Liu, Yuxiang Wang

Faculty, Staff and Student Publications

Mutations in isocitrate dehydrogenase (IDH) genes sensitize gliomas to PARP inhibition (PARPi) by inducing epigenetic reprogramming of DNA damage repair circuits. However, tumors treated with PARPi eventually relapse despite initial responsiveness. In this study, it is demonstrated that the anti-angiogenic agent lenvatinib synergizes effectively with PARPi, resulting in substantial tumor regression and significantly extended survival. Genomic analysis of tumors reveals that PARPi induces widespread transcriptomic changes that are predominantly pro-inflammatory, thereby promoting tumor angiogenesis. Prickle4, a planar cell polarity protein, is identified as a critical mediator of PARPi-induced neovascularization. Targeting Prickle4 effectively overcomes PARPi resistance in these tumors. Collectively, these …

Clinical And Molecular Characteristics Of Patients With Young-Onset And Average-Onset Pancreatic Adenocarcinoma, Adriana C Gamboa, Kever A Lewis, Laura R Prakash, Zhouxuan Li, Wei Qiao, Dan Zhao, Mark W Hurd, Mahmoud Yousef, Naruhiko Ikoma, Michael P Kim, Jeffrey E Lee, Jessica E Maxwell, Ching-Wei D Tzeng, Matthew H G Katz, Rebecca A Snyder

Faculty, Staff and Student Publications

Purpose: The incidence of young-onset pancreatic cancer (YO-PC) has risen over the past two decades, yet its molecular characteristics and long-term outcomes remain poorly defined.

Methods: We retrospectively evaluated patients with PC treated at a tertiary referral center from 2016 to 2022 who had available molecular data. Patients were classified as YO-PC (≤50 years) or average-onset PC (AO-PC, >50 years). A subset analysis examined outcomes in those who underwent curative-intent pancreatectomy. Primary end points included overall survival (OS) and recurrence-free survival (RFS).

Results: Among 511 patients, 10.9% had YO-PC (n = 56; median age 44 years). Patients with YO-PC more …

Endogenous Processes Underlying Clock-Like Mutational Signatures, Teresa Druck, Rami I Aqeilan, C Marcelo Aldaz, Nicola Zanesi, Kay Huebner

Faculty, Staff and Student Publications

Wellcome Trust scientists have shown that “mutational signatures” in specific nucleotide contexts accumulate in genomes of mammalian tissues, providing clues to underlying causes of specific signatures. Analysis of cancer genomes has identified more than 50 single‐base substitution (SBS) signatures, with SBS1, SBS5, and SBS40 linked to aging and present in normal tissues. SBS1 results from cytosine demethylation, whereas SBS5 and SBS40 arise from unknown endogenous mechanisms. We hypothesized that loss of fragile‐site genes drives these two signatures. FHIT, located at FRA3B, is frequently deleted in cancers, and Fhit‐deficient mouse tissues exhibit a mutation profile resembling human SBS5. Data mining of …

A Constraint-Based Approach To Developing Melodic Improvisational Skills In Intermediate Jazz Piano Students, Alton Xian Rong Wong

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines the pedagogical value of a constraint-based approach in helping students develop improvisational fluency and coherence by facilitating audiation. Although there are pedagogical texts on jazz improvisation that incorporate exercises shaped by constraint-based thinking, there is neither a systematic framework for their application with students of varying levels and musical backgrounds, nor a clear explanation of their pedagogical purpose. This research investigates how constraint-based pedagogy, through the lens of rhythmic, harmonic, and melodic parameters, can be applied independently or collectively to support the development of improvisational fluency in intermediate level jazz piano improvisers. It argues that musical constraints …

Investigation Of Succinylated Zwitterionic Polyethylenimine/Dna Complexes: Performance In Serum, Internalization, And Intracellular Fate, Levi Lampe

Theses and Dissertations--Chemical and Materials Engineering

Gene therapy has promised to revolutionize medicine since the earliest clinical trials, but a lack of delivery methods that are both safe and efficient has hampered progress. Gene therapy with viral vectors is effective at delivering nucleic acids to cells, but is associated with dangerous immunogenic responses and cancer-causing insertions into the genome. Viral vectors are also among the most prohibitively costly therapeutics in medicine. Polymeric vectors avoid these dangers and are made from inexpensive, off-the- shelf materials, but have been hindered by poor efficiency compared to viral vectors.

A benchmark polymer for in vitro nucleic acid delivery has been …

The Impact Of War On The Value-Legal Aspects Of State-Confessional Relations In Ukraine, Tetiana Havryliuk, Oleg Buchma, Yulia Bohdan, Anna Hud

Occasional Papers on Religion in Eastern Europe

This article analyzes the latest developments in state-confessional relations in Ukraine that have formed as a result of transformations in the current legislation on religion and religious activity. These transformations were caused by the war of the Russian state against Ukraine, which actively uses religion for propaganda purposes. Spiritual values have become an important element of this propaganda. As demonstrated in the article, they contain a motivating component and therefore play a crucial role in transforming reality. The manipulation of values allows for situations where a tragic war is declared “sacred” and even blessed. The article shows that the amendments …

Religious Radio Broadcasting In Ukraine In The Context Of Digital Mediatisation And Social Transformation, Oleh Sokolovskyi, Olena Yurchuk

Occasional Papers on Religion in Eastern Europe

The study carries out a religious studies analysis of contemporary forms of spiritual communication implemented through audio and digital media platforms. The work highlights the particular features of organizing religious broadcasting under conditions of transformation of the information space, when traditional models of transmitting doctrine become integrated into new technological formats. Mechanisms of the formation of religious identity, ways of maintaining spiritual continuity, and strategies of interaction between religious institutions and their audiences are examined. An analysis is conducted of the structures that ensure access to spiritual content, including forms of catechetical communication, mentoring practices, and models of pastoral support …

The Cross And The Flag: Papal Diplomacy And John Paul Ii’S Struggle Against The Tyranny Of The Possible: A Review, John Grondelski

Occasional Papers on Religion in Eastern Europe

A review of Lebui, John Tanyi Nquah. The Cross and the Flag: Papal Diplomacy and John Paul II’s Struggle Against the Tyranny of the Possible. South Bend, Indiana: St. Augustine’s Press, 2024. Pp. xxvi + 322. PB, $30. ISBN 978-1-58731-143-7.

Eurasianism As The Ideological Foundation Of Putin's Russia: Protestant Dimensions., Pavlo Pavlenko

Occasional Papers on Religion in Eastern Europe

This article explores the ideological foundations of contemporary Putinist Russia, identifying Eurasianism as a primary socio-philosophical and geopolitical doctrine driving the Kremlin's imperial ambitions. The author traces the evolution of Eurasianism from its origins in "White émigré" circles to its current role as a conceptual bedrock for Russian fascism. A comparative analysis is provided between Eurasianism and the "Russian World" (Russkiy Mir) doctrine, highlighting their distinct approaches to ethnocentrism and continental unity. A significant portion of the study is dedicated to the "Eurasianization" of Protestant denominations within the Russian Federation, illustrating how these communities have been instrumentalized by the state …

The Reconfiguration Of Institutional Trust In The Church In Ukraine During The Full-Scale Russian–Ukrainian War, Nataliia Ishchuk

Occasional Papers on Religion in Eastern Europe

The article analyzes the transformation of institutional trust in the Church in Ukraine under the conditions of the full-scale Russian–Ukrainian war. The phenomenon of trust is interpreted as an interdisciplinary concept that makes it possible to combine theological, anthropological, and social approaches to its comprehension. Trust in God and trust in the Church belong to different levels of religious experience: the former has an existential character, while the latter functions as a form of institutional legitimacy. Based on sociological survey data from the Kyiv International Institute of Sociology (KIIS) and the Razumkov Centre, it is demonstrated that throughout all the …

The Transformation Of Traditional And Islamic Values Of Kazakhstani Youth In The Context Of Globalization, Nurgul Kabylbayeva, Kalimash Begalinova, Mohd Roslan Bin Mohd Nor

Occasional Papers on Religion in Eastern Europe

This article provides a comprehensive analysis of the impact of globalization processes on the transformation of traditional and Islamic values among Kazakhstani youth. In the context of contemporary globalization, the expansion of the information space, intensified cultural interaction, and the spread of Western lifestyles significantly influence young people’s worldview and value orientations. The study examines how traditional values inherent in Kazakhstani society and the spiritual and cultural foundations of Islam are being transformed in the consciousness of youth. Particular attention is paid to the emergence of new value models under the influence of globalization and their impact on national identity …

The Response Of Diaspora And Religious Institutions On The Refugee Crisis: Case Study Of The Republic Of Croatia, Galyna Sagan Dr., Tetiana Kostiuk Dr.

Occasional Papers on Religion in Eastern Europe

This article is dedicated to the multifaceted support that Ukrainian diaspora and Catholic organizations in the Republic of Croatia have been providing to Ukrainian refugees since the full-scale Russian invasion on February 24, 2022. The study details the systemic response of such key religious institutions as the Diocese of Križevci, Caritas and the Jesuit Refugee Service (JRS). Particular attention is paid to integration initiatives, like educational programs developed by the Catholic University of Croatia (HKS), and the provision of spiritual and pastoral care, which remains the unique prerogative of the Church. The study emphasizes coordination between ecclesiastical and state …

Bolshevik Anti-Religious Policy As An Instrument Of Ideological Influence On The Youth Of National Minorities In The Volhynia-Zhytomyr Region (1925-1936), Galyna Starodubets, Oleksandra Stasiuk

Occasional Papers on Religion in Eastern Europe

The relevance of the research problem lies in the need to expand scholarly knowledge about the role and place of national minorities (Poles, Germans, and Jews) in the processes of Sovietization of the Ukrainian countryside and in the establishment of the totalitarian regime in general during the first decades of Soviet rule in Ukraine. Drawing on extensive source material, the article examines the policy of the Bolshevik authorities toward the youth of the Polish, German, and Jewish national minorities in the Volyn–Zhytomyr region against the background of the Sovietization of the countryside in the mid-1920s to the early 1930s. It …

Religious Education In Bulgaria: Historical Development, Values, And Public Role In Contemporary Society, Ioannis Kaminis

Occasional Papers on Religion in Eastern Europe

This paper examines the historical development, contemporary legal framework, and public debates related to religious education in Bulgaria. The analysis traces the role of Christian (Orthodox) education from the Middle Ages to the present day, paying special attention to the dramatic changes during the communist period and the transformations after 1989. At the heart of the study is the question of the place of religious education in a context of democracy, pluralism, and constitutionally guaranteed freedom of conscience. The article examines the current regulatory framework, the religious landscape in the country, and the various models for teaching religion in Bulgarian …

Theological Foundations And Regional Adaptation Of Jihadist Ideology In Russia: Case Of The North Caucasus, Tilekkabyl Amangeldi, Gulzhan Rasheva

Occasional Papers on Religion in Eastern Europe

The growth of jihadist movements in the post-Soviet space has generated significant scholarly interest in the ideological and theological mechanisms that sustain militant mobilization. Russia, particularly the North Caucasus, represents an important regional context where global jihadist doctrines intersect with local political conflicts and historical narratives of resistance. This article examines the theological foundations of Russian jihadist movements in order to understand how militant actors reinterpret Islamic doctrines to legitimize violence and construct ideological authority. The study aims to analyze the key theological concepts that shape jihadist discourse in Russia and to explore how global Salafi-jihadist ideology has been adapted …

The Autonomy Of Bulgarian Theological Scholarship: Institutional Control And Discursive Transformation Under The Communist Regime (1944–1960s), Diana Petrova Tyurkedzhieva

Occasional Papers on Religion in Eastern Europe

The article examines the autonomy of theological scholarship in Bulgaria under the communist regime as part of the broader problem of academic freedom under authoritarian rule, focusing on 1944 to the 1960s. While existing research has primarily addressed the relationship between the Orthodox Church and the State through institutional restriction and political repression, this study analyses how theological scholarship was transformed institutionally and discursively. It combines historical and institutional analysis with an examination of speeches, public statements, and academic publications in ecclesiastical and theological periodicals. The article argues that the separation of the Faculty of Theology from Sofia University in …

Italian Missionary Activities In Georgia: Historical Legacy And Modern Inter-Religious Dialogue, Zaza Tsotniashvili

Occasional Papers on Religion in Eastern Europe

This article examines the historical development, cultural contributions, and contemporary significance of Italian missionary activities in Georgia spanning seven centuries, from medieval Catholic presence through the traumatic Soviet period to the post-independence revival. Drawing upon Georgian, Italian, and Vatican archival sources, including the manuscripts of Arcangelo Lamberti, Cristoforo de Castelli, and Michel Tamarati, the study provides comprehensive analysis of Italian Catholic missionaries' multifaceted engagement with Georgian society. The research demonstrates that Italian missionaries, particularly members of the Theatine, Capuchin, and Augustinian orders, played roles extending far beyond religious evangelization, functioning as diplomatic intermediaries, medical practitioners, linguistic documentarians, ethnographic observers, and …

Imperial Islam And Colonial Control: The Orenburg Muslim Spiritual Assembly As A Mediator Of Russian Rule In The Kazakh Frontier, Zharas Akhan

Occasional Papers on Religion in Eastern Europe

Examination of the strategic role of the Orenburg Muslim Spiritual Assembly (OMSA) as a pivotal instrument of imperial governance in the Kazakh Steppe during the late eighteenth and nineteenth centuries. The study investigates how the Russian Tsarist administration instrumentalized Islamic institutions to exercise political control and facilitate the integration of nomadic Kazakh society into the imperial hierarchy. Drawing on extensive archival evidence, the author analyzes the institutional evolution of the Muftiate, its involvement in diplomatic and intelligence operations, and the intermediary agency of Tatar mullahs who bridged the gap between the Kazakh aristocracy and the Russian bureaucracy. The paper identifies …

Use Of Courts By The Totalitarian Regime Against Protestant Communities In Ukraine, Oksana Vysoven, Natalia Varadi, Yurii Fihurnyi, Ganna Kriukova

Occasional Papers on Religion in Eastern Europe

This article analyzes the use of the courts by the totalitarian regime against Protestant communities in Ukraine. It has been established that the show trials held in the 1960s–1980s against the leaders and active members of the Council of Churches of Evangelical Christian Baptists, which opposed the totalitarian regime, and against ordinary believers in Protestant communities were conducted according to the plan of atheistic campaigns by officials of the communist regime and were organized by the repressive special services of the State Security Committee and the Ministry of Internal Affairs. It has been established that the trials led to the …

Pentecostals In The Ukrainian Ssr During Collectivization And The Holodomor Of 1930–1933: Socio-Adaptive Survival Practices, Oleksandr Korotaiev

Occasional Papers on Religion in Eastern Europe

The article examines the social adaptation and survival practices of Pentecostal communities in the Ukrainian SSR under the conditions of forced collectivization, dekulakization, and the Holodomor of 1930–1933. Drawing on criminal-investigative case files of the GPU, top-secret Soviet security service documents, party and state decrees, and testimonies of contemporaries, the study reconstructs individual, family, and communal strategies employed by believers in response to systematic state violence. Particular attention is devoted to Pentecostal communities in the Odesa okrug, especially the village of Holma in the Balta district, where Soviet repressive policies manifested themselves through a combination of economic pressure, religious persecution, …

Marriage Governance In Kyrgyzstan: Convergence And Divergence Between Secular Law, Islamic Norms, And Customary Practices, Aisuluu Adylbekkyzy, Zailabidin Azhimamatov, Aiitmamat Kariev

Occasional Papers on Religion in Eastern Europe

Marriage governance in Kyrgyzstan is shaped by the coexistence of secular law, Islamic norms, and customary law), creating a plural legal environment in which multiple sources of authority regulate family relations. This study examines how these normative systems interact in the governance of marriage, identifies areas of convergence and divergence, and explores their implications for legal legitimacy and family regulation. Employing a qualitative socio-legal methodology grounded in legal pluralism theory, the research draws on documentary and comparative analysis of legal texts, Islamic sources, official religious guidance, and relevant scholarly literature. The findings demonstrate that marriage governance in Kyrgyzstan operates through …

Oral Religious Mediation And Transmission Of Islamic Discourse In The Kazakh Steppe: Case Of Syrdarya Poets, Nurzhan Darkulov

Occasional Papers on Religion in Eastern Europe

This article investigates the role of Syr Darya poets as mediators of Islamic ethical and religious discourse in the nineteenth- and early twentieth-century Kazakh steppe. The analysis demonstrates that these poets occupied an intermediary position between broader Islamic traditions and local communities, translating ethical teachings, theological ideas, spiritual reflection, and social criticism into culturally accessible forms of oral expression. Their influence extended beyond literary activity, functioning as a socially recognized mode of religious mediation within a decentralized religious environment. The study argues that oral poetic performance constituted an important mechanism through which Islamic knowledge, moral norms, and religious values were …

Computational Insights Into Orthotropic Fracture: Crack-Tip Fields In Strain-Limiting Materials Under Non-Uniform Loads, Saugata Ghosh, Dambaru Bhatta, S. M. Mallikarjunaiah

School of Mathematical & Statistical Sciences Faculty Publications

A finite element framework is presented for analyzing crack-tip phenomena in transversely isotropic, strain-limiting elastic materials. Mechanical response is characterized by an algebraically nonlinear constitutive model, relating stress to linearized strain. Non-physical strain singularities at the crack apex are mitigated, ensuring bounded strain magnitudes. This methodology significantly advances boundary value problem (BVP) formulation, especially for first-order approximate theories. For a transversely isotropic elastic solid with a crack, the governing equilibrium equation, derived from linear momentum balance and the nonlinear constitutive model, is reduced to a second-order, vector-valued, quasi-linear elliptic BVP. This BVP is solved using a robust numerical scheme combining …

Demographic Characteristics In Patients With Heart Failure And Iron Deficiency, Ahmed Alaa Eldin Abd Elazeem, Abdul Razek Abdul Lateef Maaty, Hanan Ahmed Azzam, Ahmed Mohamed Reda

Mansoura Medical Journal

Background: Iron deficiency (ID) is a major and prevalent comorbidity among heart failure (HF) patients. It is associated with poor exercise tolerance, recurrent hospitalization and higher mortality. Studying patients’ demographics helps to determine vulnerable groups to ID, risk factors and associated comorbidities to be considered during screening and management.

Aim of the Work: To study the demographic characteristics of heart failure patients with iron deficiency.

Patients and Methods: A cross sectional observational study was conducted at a tertiary care university hospital at Delta region of Egypt between year 2024 and 2026. Among 117 HF patients, 100 HF patients …

Front Matter

SMU Law Review

No abstract provided.