Digital Commons Network^™

Bacterial Purification Of Hat Enzymes From Cryptococcus Neoformans: Esa1 And Gcn5 Purification, Manasi Joshi, Andrew Wisley

Liberty University Research Week

Gene expression in eukaryotes is tightly regulated by post-translational modifications. Histone acetylation is an epigenetic modification associated with increased transcription. The putative histone acetyltransferase (HAT) in the fungus Cryptococcus neoformans, Something About Silencing 3 (SAS3), has been a novel research target due to its potential role in virulence that causes nearly 112,000 to 625,000 annual deaths worldwide through cryptococcal meningitis. Although SAS3 homologs acetylate core histone 3 in mammalian organisms, recombinant SAS3 has not yet demonstrated HAT activity. Previously, an established human HAT p300 was used as a positive control to validate enzymatic conditions for HAT assays. However, using known …

Offspring Cognitive And Neurobiological Outcomes Influenced By Maternal Dietary Patterns, Emma Whatley, Abigail Frommack, Karina Long, Sarah Nelson

Liberty University Research Week

The impact of maternal diet on offspring neurodevelopment is a relatively new field, and its areas of focus are vast. The field is predominantly studied and understood through the impacts of an unhealthy diet, the maternal Western diet (WD), but there is very little research on healthy diets like the maternal Mediterranean diet (MD). The general healthiness of the diet and some limited research support that this diet could have beneficial impacts on offspring. This research aims to explore the impacts of a maternal MD on adult offspring. We hypothesize that the maternal MD will enhancing cognitive performance in learning, …

Impacts Of Maternal Diet On Offspring Affective Behavior And Brain Morphology, Abigail Frommack, Emma Whatley, Karina Long, Sarah Nelson

Liberty University Research Week

The field of maternal dietary impacts on offspring neurodevelopment has been established over the past 30 years, and since its establishment it has grown rapidly. The research shows that maternal diet significantly impacts offspring neurodevelopment, with those effects lasting until adulthood. Most research in this field focuses on the detrimental impacts of a Western Diet (WD), but little research exists on the beneficial impacts of a healthy diet. This research aims to close that gap by assessing a healthy diet and its impact on adult offspring. We hypothesize that a maternal Mediterranean diet (MD) will have positive impacts on the …

Single Step Cyanoacrylate Fumigation With Common Dyes, Abigail Proffitt, Jared Filegar

Liberty University Research Week

Cyanoacrylate fumigation is a widely used fingerprint development method to visualize latent fingerprints left on nonporous surfaces. This methodology relies on heating cyanoacrylate, otherwise known as superglue, to create vapors that polymerize onto fingerprints. These vapors react via biological components such as amino acids and other residues left from finger oils. This technique is very effective; however, once the polymer has completed its reaction the fingerprint is colorless resulting in the need for a secondary staining technique. The additional procedure increases processing time and introduces potentials for smearing or human error. This research aims to investigate a way to consolidate …

Characterization Of The Aspergillus Flavus Rdia Gene With A Rdi1∆ Mutant Of C. Neoformans, Sam Raub

Liberty University Research Week

The genus Aspergillus includes several species that are opportunistic pathogens, with members ranked in the World Health Organization’s fungal critical priority pathogen list. Aspergillus flavus, a member of the genus, synthesizes aflatoxin (AF), a polyketide mycotoxin and the most potent naturally occurring carcinogen known. aflR is a pathway-specific transcription factor that plays a central role in the regulatory network for AF biosynthesis. Transcriptomic analysis under AF-conducive and non-conducive conditions identified a cluster of genes with similar expression profiles to aflR. Among these is a putative Rho-GDP dissociation inhibitor that regulates AF production and virulence in A. flavus. Deletion of this …

Analysis Of The Effects Of Phytocannabinoids Cannabichromene (Cbc), Cannabidiol (Cbd), And Cannabigerol (Cbg) On The Enzyme Faah, Rachel Duncan

Liberty University Research Week

No abstract provided.

Histological Quality Of Fresh And Pre-Fixed Tissue, Rebekah Satalino, Andrew Hodges, Bright Danso, Gretchka Mair, Kingsley Ampong

Liberty University Research Week

The quality of a tissue sample used for histological analysis robustly influences the accuracy of scientific results and diagnostic interpretation. For fresh tissue samples, the duration between termination and fixation is a major determinant for tissue quality going forward as fixation halts tissue degradation. The condition of the fixed tissue before subsequent processing can have a pronounced effect on staining characteristics and cellular component integrity as examined under the microscope. However, while fresh tissue is frequently considered best, many institutions may only have access to pre-fixed tissue for training or research purposes. The aim of this research is to compare …

Canndor Herbarium: A Comprehensive Herbarium Resource For Cannabis Identity, Diversity, And Botanical Research, Nick Batora, Laura Klein, Kerin Law, Eleanor Kuntz

Oxford ICSB

Decades of cannabis prohibition have created major gaps in our understanding of cannabis biology, especially with regard to the genomic, phenotypic, and germplasm resources that are standard in other agriculturally and medicinally important crops. Herbarium collections, which serve as archival specimens that preserve plant material for long term study, are no exception. Although herbarium specimens are meant to document plant diversity, most collections contain only a narrow sampling of cannabis and do not reflect the extensive variation present in commerce or this species at large. To address this need, the Canndor Herbarium was established in 2016 as the first dedicated …

Mechanistic Insights Of Enterolactone Within 3t3-L1 Adipocyte Cells, Merritt Smith

Liberty University Research Week

Type 2 diabetes mellitus (T2D) is one of the leading causes of morbidity and mortality in the United States, with approximately 25% of healthcare expenditures directed toward its management and associated metabolic complications. In response to the urgent need for novel therapeutic strategies, recent research has focused on bioactive compounds derived from dietary sources. In light of that, we identified the plant lignan matairesinol (MA) and its gut-derived metabolite, enterolactone (ENL), as agents capable of enhancing glucose uptake in 3T3-L1 differentiated adipocytes. These results suggest that enterolactone may represent a promising dietary supplement for improving glycemic control in patients with …

Evaluating Sexual Dimorphism In An Urban Neonate Regina Septemvittata Population, Arleigh Wagoner, Nathan Wilkins

Liberty University Research Week

Regina septemvittata, commonly known as Queensnakes, are broadly distributed across eastern and central North America. In adult Queensnakes, females average a greater snout-vent length (SVL) than males. Since Queensnakes exhibit size dimorphism and have short life spans and low survival rates, it was hypothesized that neonate Queensnakes begin to display a distinct sex-related difference in SVL within their first few months of life. Our study investigated growth rates of neonate Queensnakes by conducting a capture-mark-recapture (CMR) study over a three-month period (August through October; birth to first brumation) in an urban stream in Lynchburg, VA. Captured snakes were measured (SVL), …

An Evaluation Of White-Spotted Bamboo Shark (Chiloscyllium Plagiosum) Development, Madison Ashby, Alexa Ashby, Baylis Huckabay, Cyrus Harris, Emma Bron, Lydia Harris, Maia Dieken, Oliver Thomas, Will Chelberg

Liberty University Research Week

An inhabitant of the north Indo-Pacific Ocean, the whitespotted bamboo shark (Chiloscyllium plagiosum) is a demersal, ambush predator of fish and invertebrates. Despite the known abundance of C. plagiosum, there is limited diagnostic information on their growth beyond embryonic development and hatching. A population of this species under managed care provides a unique opportunity to chart growth from the respective stages of life such as egg hatchling, sub-adult, and adult life stages. Through this research, we set out to chart the growth parameters on three hatchling bamboo sharks from a weekly data collection. These parameters include weight (g), sex, pre-caudal …

Evaluation Of Cadaver Tissue Antigenicity As A Practical Teaching Tool For Immunohistochemistry, Shanice Rerrie, Jumana Rizk, Levon Shukrian, Maria Mendoza Lopez

Liberty University Research Week

Immunohistochemistry (IHC) is a core technique in biomedical research and is increasingly important for teaching at the undergraduate and graduate levels. However, teaching laboratories typically rely on animal-derived tissues because the access to fresh human specimens is restricted due to ethical concerns. The purpose of this study was to evaluate whether cadaveric tissue can retain sufficient antigenicity for immunohistochemical analysis and thereby serve as an effective and ethical teaching resource. Liver lobe and renal cortex samples (approximately 1 cm³) were collected from an 88-year-old female donor. Target proteins were selected for their established roles in vitamin D metabolism and their …

Formation Of Rosette-Like Hierarchical Structures On Cylindrical Titanium Surfaces., Cole Urquhart, Ally Doud, Michael Korn, Hector Medina

Liberty University Research Week

The purpose of this experiment was to characterize titanium-based rosettes and other micro-sized crystal formations on multiple different non-planar titanium surfaces. Rosettes are crystal structures in the titanium sulfate family. They are 20-150µm in diameter and vastly increase the surface area of the places where they are grown, which can lead to various possible applications in future research. The titanium type that was being experimented on was titanium gauze (50µm diameter). To grow titanium-based structures, two steps are involved: etching and crystal formation. Firstly, the titanium material was placed in a centrifuge tube filled with 1mL of 18M (96%) conc. …

Investigating The Natural Communities And Flora Of Candler’S Mountain, Lydia Harris, Cyrus Harris, Tyler Knick

Liberty University Research Week

Candler’s Mountain is home to a variety of natural communities and flora. With more than 1200 vascular plant species documented in Campbell County, the identification and growing historical record of local flora will support short and long-term recognition of the importance of biodiverse natural communities in central Virginia. This project seeks to (1) document flora diversity in this region, (2) further establish an herbarium teaching and research collection of representative species, and (3) advance our understanding of the flora in relation to natural communities. Identified ecological groups and community types are based upon the Virginia Department of Conservation and Recreation …

Sem-Edx Analysis Of Animal Hairs For The Presence Of Metals, Sydney Stevens, David Green, George Monroe

Liberty University Research Week

No abstract provided.

Comparison Of Hypothalamic And Hippocampal Neurogenesis Across Sexes And Ages Of Mice, Chloe Jensen, Madison Reynolds, Rebecca Kinyon

Liberty University Research Week

Background: The hippocampus is primarily known for its function in learning and memory; thus, it makes sense that this is a well-established highly neurogenic zone. Recent studies have demonstrated that the hypothalamus also has neurogenic properties where there were once believed to be none. Because of these recent findings, our study aims to provide baseline data pertaining to rates of neurogenesis in the hypothalamus in comparison to the hippocampus along with distinguishing differences in these rates between males and females. Methods: Using four mice, three young and one aged female, we cryosectioned the brain to image the hippocampal and hypothalamic …

Spectroscopic Characterization Of Pfas Ligands Binding To Novel Plant Peroxidases, Vladimir Bogatov

Liberty University Research Week

No abstract provided.

Elephant's Toothpaste: A Statistical Investigation Of The Yeast-Catalyzed Decomposition Of Hydrogen Peroxide, Annaliese Defelice

Liberty University Research Week

Catalase is the main enzyme in yeast that catalyzes the popular “elephant’s toothpaste” reaction. Elephant’s toothpaste reactions often proceed using an iodide or yeast catalyst, but iodine is favored for ease of quantitative analysis. There remains little statistical data on the yeast-catalyzed hydrogen decomposition reaction. The relationship between reagent amounts and reaction rate was measured by manipulating reagent quantity while maintaining other variables. The results were graphed and fitted for linearity, indicating the relative relationship between each reagent and the overall reaction rate. There were strong positive correlations between yeast and hydrogen peroxide concentrations and reaction rate. Augmenting the volume …

Allosteric Modulation Of Faah By Tocopherols, Beatrice Pyles

Liberty University Research Week

No abstract provided.

Enzyme Kinetics Of Faah, Susanna Stell, Emma Hooks

Liberty University Research Week

Anandamide (AEA) is an endogenous ethanolamide that functions as a signaling molecule within the endocannabinoid system, a regulatory network processes including pain perception, mood regulation, appetite, and neural signaling. AEA belongs to a broader class of fatty acid ethanolamides naturally present in the body, which also includes palmitoylethanolamide (PEA) and oleoylethanolamide (OEA). The activity of AEA is tightly regulated through enzymatic degradation by fatty acid amide hydrolase (FAAH), which hydrolyzes AEA into arachidonic acid and ethanolamine, thereby terminating its signaling activity. The purpose of this study is to investigate the rate at which AEA is hydrolyzed by the FAAH enzyme …

Pilot Study: Black Tea Extract And Theaflavin Polyphenols Effects On L6 Myotubes, Elizabeth Allen

Liberty University Research Week

This research investigates bioactive polyphenol group theaflavins found in black tea and how they affect Akt signaling in skeletal muscle. The black tea polyphenols are a collection of innate compounds in Camillia sinensis, among them being the theaflavin group which is comprised of four compounds: theaflavin (TF1), theaflavin 3-gallate (TF2A), theaflavin 3’-gallate (TF2B), and theaflavin 3,3’ digallate (TF3) (He, 2017). The theaflavins group has been described as bioactive in a variety of different cellular pathways such as apoptosis, cellular division, gene expression and nutrient regulation (Li et al., 2021)(He, 2017)(Lin et al., 2007).

Previous work by Nagano et al., demonstrated …

Impacts Of Interspecific Competition On Stress And Body Condition In A Montane Salamander (Plethodon Hubrichti), Jason Porter, Allison Carmen

Liberty University Research Week

No abstract provided.

Quantifying Biogenic Amines In Anaerobic Porcine Liver Decomposition For Improved Pmi Estimation Using Gc/Ms, Shanice Rerrie, Faith Ekoh

Liberty University Research Week

Postmortem interval (PMI) estimation remains a central challenge in forensic science, particularly during the early stages of decomposition. Quantifying biochemical changes during tissue breakdown has emerged as a promising approach to improve accuracy in PMI determination. This study expands on earlier research that quantified the biogenic amines cadaverine (CAD), putrescine (PUT), and methylamine (MEA), which result from bacterial amino acid decarboxylation during decomposition. It specifically examines how these compounds form under anaerobic conditions over a prolonged 30-day period. Porcine liver, serving as a human tissue analog, was decomposed in sealed anaerobic chambers. Tissue samples were collected at regular intervals, homogenized, …

Music Enhances Theta Brainwave Activity Regardless Of Tuning Frequency, Robert Somerville, Davis Raz, Ersilia Mirabelli, Jettson Yarborough, Luc-Shalom Kouame

Liberty University Research Week

No abstract provided.

“Slaves To Sin:” Harnessing Neuroplasticity To Defeat Habitual Behaviors, Charity Parrott

Liberty University Research Week

How does neuroplasticity affect the life of a Christian? This thesis will seek to address the concepts of habit formation, neuroplasticity, and top-down control through a neurobiological perspective, and how they relate to the Biblical concept of habitual sin. Discoveries in neuroplasticity have revealed the brain to be a malleable organ, capable of rewiring neural connections and, in the hippocampus region, neurogenesis, or the creation of new neurons (Fuchs & Flügge, 2014). This thesis will compile research in neuroplasticity, explore the biological reasons behind the difficulty in escaping patterns of sin, and connect Biblical-based strategies for sin prevention with neurobiology. …

Examining Carroll’S Alice In Wonderland And Through The Looking Glass, And What Alice Found There: A Christian Calling To Spiritual Childhood, Kristen Hess

Liberty University Research Week

"Examining Carroll’s Alice in Wonderland and Through the Looking Glass, and What Alice Found There: A Christian Calling to Spiritual Childhood" dives into the positive and negative childlike habits that Alice, a representative of children and childhood, embodies and how Christians should identify and emulate her positive childlike behaviors in order to best fulfill their roles as spiritual children of God. Because Christians are children of God according to Romans 8:16, it is vital for Christians, like children, to understand the world around them, learn about themselves and others, and enjoy physical and mental play and recreation. Lewis Carroll found …

Paws And Predictions: Interpreting Intraspecific Cat Social Interactions Through Facial Signals, Levi Neal, Lauren Scott, Gosia Zobel, Brittany Florkiewicz

People and Animals: The International Journal of Research and Practice

There has been limited research on humans’ ability to perceive and predict social interactions among cats using facial signals. This area is crucial to explore since conflicts between cats often cause significant stress for their guardians. By evaluating individuals’ skills in understanding cat interactions, researchers can develop tools to help guardians predict affiliative and nonaffiliative behaviors, leading to effective intervention strategies for multicat households. We distributed a survey to individuals subscribed to the Companion Animals New Zealand newsletter to assess cat guardians’ effectiveness in perceiving and predicting various social interactions among cats. We hypothesized that guardians with one or more …

Real World Fertility: From Participatory Research To Clinical Trial, Belinda Tan, Azure Grant, Ayla Barmmer

Oxford ICSB

Nutritional supplements are widely popular for female reproductive health, but their ingredients are typically studied in isolation in the laboratory. Finished products make claims based on these isolated studies, creating a gap in which impact of multi-nutrient formulas in the real world are not investigated, largely due to high research costs. Decentralized clinical research methods are modernizing clinical trials, leading to affordable, efficient evidence generation in large representative populations. Utilizing a decentralized research platform, we tested the hypothesis that specific supplement protocols would be associated with improved female health and fertility in a real world cohort. We present a novel …

2026 Acssc Program, Acssc Planning Committee

Annual Celebration for Student Scholarship and Creativity

No abstract provided.

Frankincense (Boswellia Spp.) Essential Oils: Chemistry-Driven Authentication, Chemotype Discovery, And Sustainability Insights Across My Published Studies, Prabodh Satyal

Oxford ICSB

Frankincense, which comes from the oleo-gum resins of Boswellia species, is one of the most important botanicals in global trade. However, its essential oils often face issues like variations in chemical makeup, mislabeling, and adulteration. This presentation summarizes findings from ten peer-reviewed studies conducted between 2016 and 2023. These studies create a chemistry-based framework for authenticating frankincense and ensuring sustainable sourcing.  Research on various Boswellia species, including B. sacra, B. carteri, B. papyrifera, B. frereana, B. occulta, B. ogadensis, and B. rivae, shows strong patterns in volatile profiles at both the species and tree levels. This supports distinguishing between species …