Digital Commons Network^™

Fluorescence-Based Quantification Of Hepatic Ldl Uptake And Donor Hepatocyte Engraftment Using Atto 700–Labeled Ldl, Victor Agustin Cortes Flores

All Theses

Familial hypercholesterolemia (FH) is an inherited disorder characterized by elevated low-density lipoprotein cholesterol (LDL-C) that increases the risk of premature atherosclerotic cardiovascular disease. In severe FH, including low-density lipoprotein receptor (LDLR), hepatic LDL clearance is profoundly impaired and many lipid lowering treatment strategies have limited effectiveness. Current advances in ex vivo CRISPR–Cas9 hepatocyte gene editing in vivo and ex vivo approaches for treatment of FH are under development.

However, a central barrier to translation is the ability to quantitatively measure the percentage of healthy gen edited hepatocytes in the liver while simultaneously assessing functional hepatic LDL handling. This thesis establishes …

Forest Overstory Mortality & Recruitment Patterns Eight Years Following Wildfire In The Southern Appalachians, Ruth Cumberland

All Theses

In this study, we investigated patterns of forest overstory mortality and recruitment eight years after wildfire in a large wildfire site the Southern Appalachians, a topic that has not been thoroughly investigated to date. With evidence that pre-fire drought promotes post-fire mortality through impaired physiological function and increased fuel consumption, unknown patterns following wildfire are important to study under shifting climate and land-use conditions. In Chapter 1, three areas of interest were investigated: (a) functional group mortality response to wildfire effects, (b) temporal differences in early (1-3 years) and late (5-8 years) periods of delayed forest overstory mortality patterns following …

Polydactyl Precursors: Insights Into Early Tetrapod Locomotion From Polydactyl American Alligators, Trevor P. Brewington

All Theses

Several of the earliest tetrapod lineages exhibited polydactyl limbs, with seven or eight digits per limb rather than the typical five or fewer seen in extant lineages. How these digit configurations might have been used during terrestrial locomotion is unclear, given the lack of living models in which their performance can be studied and limited evidence from fossil trackways. However, polydactyly occasionally appears in natural populations of American alligators (Alligator mississippiensis), animals with body plans that broadly resemble those of early tetrapods. To test how polydactyl limbs similar to those of early tetrapods might have functioned on land, …

Modeling Repeated Evolution Of Polygenic Traits, Marshall N. Hoskins

All Theses

Understanding the genetic composition of adaptive evolution is a longstanding and primary goal of evolutionary genetics. Despite many advances in our knowledge of trait adaptation, it remains unclear how demographic changes and repeated selection might alter the expected genetic architecture of adaptation. We used forward-in-time genetic simulations to test and observe the effects of opposing, repeated selection regimes on a polygenic trait in populations with and without changes in demography to better understand the genetic alleles that underlie polygenic adaptation. To further illuminate how natural populations might evolve, we investigated the impact of mutation effect size, the role of dominant/recessive …

The Invisible Toll: Black College Women And The Intersection Of Academic Stress, Rest Deficiency And Exhaustion At Clemson University, Jomya Ford

All Theses

Leisure has been an under-explored aspect of Black women’s lives in higher education, particularly within predominantly white institutions (PWIs), where limited culturally relevant opportunities and racialized isolation shape daily experiences. Constraints around rest, rooted in racism and gendered expectations, continue to influence how Black college women navigate academic pressure, deficiency narratives, and exhaustion. Guided by intersectionality, this qualitative study draws on semi‑structured interviews with Black undergraduate and graduate women at a PWI to explore how cultural upbringing, institutional climates, and internalized expectations shape their definitions and practices of rest. Findings show that while participants recognize rest as necessary, they rarely …

The Prevalence Of Amenorrhea In Relation To Red-S Syndrome Among Various Sports In Collegiate Female Athletes, Ruby S. Holland, Marilou D. Shreve

The Eleanor Mann School of Nursing Undergraduate Honors Theses

Background: RED-S is a common risk factor for amenorrhea in female athletes. Athletes that compete in high-intensity sports or without adequate caloric intake, are at risk for low energy expenditure which may disrupt hormonal balance. As development of RED-S is a well-established risk factor for amenorrhea, early detection and correction is crucial to prevent long term health consequences.

Purpose: The purpose of this research is to explore if there is a correlation between RED-S score and amenorrhea in female athletes.

Methods: An online survey was distributed to approximately 220 female athletes at the University of Arkansas using Qualtrics. The 69-question …

Effects Of Beaver Dams On Components Of Riverscape Health And Biodiversity In The Great Basin, Cashe C. Rasmussen

All Graduate Theses and Dissertations, Fall 2023 to Present

Networks of rivers and their floodplains (i.e., low-lying areas adjacent to rivers and streams that could plausibly flood) have been degraded, reducing the availability of freshwater and habitat for people and wildlife. Therefore, land managers are pursuing cost-effective restoration strategies, including partnering with beavers (Castor canadensis), to promote the health of these ecosystems and associated benefits. However, our understanding of beaver-mediated restoration benefits remains incomplete. Here, we compared locations along streams with and without beaver dams to measure the benefits of beavers in the Great Basin of the Western United States. We measured the effects of beaver dams …

Grazed Pasture Effects On Greenhouse Gas Emissions In The Ozark Highlands, Tyler Buchanan

Graduate Theses and Dissertations

In the United States (US), grazinglands serve as the main foundation for the livestock industry, and, in Arkansas specifically, pastures are essential for rotational grazing and dairy operations. Climate change is increasingly becoming a concern in the agriculture industry, largely caused by anthropogenic activities increasing greenhouse gas (GHG) concentrations in the atmosphere, at least partly due to the nutrient recycling that occurs from animal manure additions in grazinglands. Therefore, quantifying GHG emissions from pastures is essential to better understanding the impacts of grazing on the environment. The objective of this study was to quantify and evaluate the potential effects of …

Dissecting The Complex Genomic Landscape Underlying Oxidative Stress Resistance Using Natural Variation In Yeast, Sonali Lakshika Anne Lenaduwe

Graduate Theses and Dissertations

Stress is experienced across the tree of life. Survival in fluctuating environments depends on a robust genetic architecture. This dissertation examines the genetic and metabolic determinants underlying one such adaptive response: cross-protection. Leveraging natural variation in the stress responses of budding yeast, Saccharomyces cerevisiae, I examine the genetic variation contributing to ethanol-induced cross-protection against oxidative stress. Acquired stress resistance, in which mild pre-exposure improves survival to subsequent stresses, is prevalent across diverse organisms and can involve both same-stress and cross-protection. Using high-throughput bulk segregant analysis (BSA-seq) and advanced intercross mapping populations derived from natural yeast isolates, I identify quantitative trait …

An Assessment Of The Basihyal And Its Functional And Ontogenetic Relationships To The Masticatory Apparatus In Primates, Parker Mitchell Taylor

Graduate Theses and Dissertations

The hyoid is central to many important functions such as feeding, vocalization, and breathing. However, primate hyoid morphology has been widely understudied compared to other aspects of craniofacial morphology. This dissertation aimed to bridge gaps in the current understanding of primate hyoid morphological variation and the hyoid’s relationship with mandibular morphology. Using microCT, diceCT, and three-dimensional geometric morphometric methods, this work details broad taxonomic variation of basihyal shape, resting hyoid position, and suprahyoid muscle architecture in primates, and assesses such variation for the effects of phylogeny, allometry, and covariation between these elements and mandibular shape. These analyses were also conducted …

Mechanisms Of Bacterial Glucokinase Inactivation Through Site-Specific Lysine Modification And Chemical Inhibition, Joseph Robert Will Andrews

Graduate Theses and Dissertations

Glycolysis and related metabolic pathways are controlled by various post-translational modifications (PTMs) that affect enzyme activity and metabolic flow. In E. coli, glucokinase (GLK) sits at the crossroads of glycolysis and the pentose phosphate pathway and carries out the first committed step of glycolysis: ATP-dependent phosphorylation of glucose. While it's known that glucose binding causes GLK to close its domains, how phosphate binding, lysine modification, and small-molecule inhibitors regulate GLK's shape and function is still not fully understood. Here, I combine structural biochemistry and enzymology to figure out how phosphate binding, specific mutations, and chemical inhibitors affect E. coli GLK. …

2025 Fact Sheet, Clemson University

Fact Sheets

No abstract provided.

Effects Of Modifications To The Contextual Fear Conditioning Paradigm On Context Discrimination Deficits In Mice, Aditi Malpure

Honors Scholar Theses

Post-Traumatic Stress Disorder (PTSD) is a psychiatric disorder that can develop following exposure to a traumatic event and is often characterized by impaired contextual discrimination, in which fear responses generalize to safe environments that resemble the original traumatic context. Previous studies have shown that elevated stress hormone levels during traumatic learning can impair memory specificity and promote generalized fear responses. The dentate gyrus (DG) of the hippocampus and adult-born granule cells (abGCs) are thought to play important roles in contextual discrimination and memory specificity. This study used contextual fear conditioning (CFC) paradigm in mice to investigate how enhanced stress and …

Herbivory Of Phragmites Australis Along A Salinity Gradient In Connecticut Wetlands, Sydney H. Kolz

Honors Scholar Theses

Invasive Phragmites australis (common reed) is widely distributed in New England wetlands, where it often outcompetes native plants and decreases biodiversity throughout the food web. Controlling its invasion is a priority, especially in salt marsh habitat, but current management techniques are inefficient. Leveraging natural processes may improve control strategies, but in order to do so, we must first better understand how abiotic and biotic stressors interact and affect Phragmites. This study focuses on herbivory and salinity, two factors limiting Phragmites’ fitness. I measured soil salinity and quantified the damage of three herbivore guilds (i.e. gall-formers and stem-borers, leaf-chewers, and …

Increasing Student-Athletes’ Awareness On Mindset, Kayla A. Young

Capstone Projects and Master's Theses

Many college student-athletes lack awareness of  the benefits associated with maintaining a growth mindset. Individuals who are unaware may have lower resilience, poor motivation, and poor habits. College students-athletes who develop and maintain a growth mindset are likely to engage in good decision making and sustain their drive towards success, academically and athletically. In order to address this issue, I have created a one-day workshop for female college track and field student-athletes at California State University Monterey Bay in Seaside, California.

Raising Regulated Moms: Reducing Reactive Parenting Responses, Victoria J. Perez

Capstone Projects and Master's Theses

Many parents and caregivers lack access to information and support that accurately relates effective parenting styles, without this knowledge caregivers and parents may unintentionally rely on reactive responses that end up escalating conflict with their child rather than resolving it. When parents respond to their child's big emotions without effective tools to first regulate themselves, they risk contributing to higher stress levels, emotional withdrawal, and behavioral dysregulation in their children. Research consistently demonstrates that when parents are given the tools to successfully incorporate self-regulation skills and evidence-based approaches to guide children through difficult emotions, both the child and the parent …

Navigating New Environments: Acculturative Stress And Substance Use Among International Vs Domestic Students From Latino/A Backgrounds, Madelyn R. Null

Psychological Science Undergraduate Honors Theses

College students can experience a range of cultural and social pressures that could relate to their alcohol and cannabis use. Latino/a international students may face unique challenges as they adjust to campus environments in the United States (U.S.), yet little research has compared their substance use patterns to those of Latino/a domestic students. Therefore, the present study aims to examine whether: (a) Latino/a international and domestic students differ in their alcohol use, (b)they differ in their cannabis use, and (c) acculturative stress moderates the relationship between international student status and these substance use behaviors. Latino/a undergraduates (n=440; Mage=20.55; female=71%; international=21%) …

The Role Of Floodplain Connectivity In Shaping The Abundance, Size-Class Distribution, And Schooling Behavior Of The Associated Fish Assemblage, Adam H. Quade

LSU New Orleans Theses and Dissertations

The life histories of many Louisiana fishes are closely tied to the timing, magnitude, and duration of the Mississippi River flood pulse, which regulates the ecological connectivity and productivity of adjacent floodplain habitats. Anthropogenic modifications such as levee construction and water-control structures have disrupted these natural hydrologic linkages, altering habitat quality and community structure across the Lower Mississippi River Basin. This dissertation used high-resolution imaging sonar to investigate how fish assemblages, species, and collectives respond to changes in floodplain connectivity within the Richard K. Yancey Wildlife Management Area prior to hydrologic restoration. In the first chapter, I applied imaging sonar …

Structural Evidence For Innervation Of A Single Epitheliomuscular Cell By Multiple Sensory Neurons In The Sea Anemone Nematostella Vectensis, Aparajita Das

Graduate Theses and Dissertations

Abstract Here, I report new ultrastructural evidence for multineuronal innervation (many-one) of a single epithelia-muscular cell in sea-anemone cnidarian Nematostella vectensis. Using Serial Block-Face Scanning Electron Microscopy (SBF-SEM) and ImageJ, I found that EMCs are located at the base of ectoderm, with an elongated cell body that includes basal processes, a bilobed nucleus, and myofibrils aligned parallel to the basal surface along with their structural quantification. Notably, I found multiple mechanosensory neurons form synapses with a single EMC. These findings enhance our understanding of cnidarian neuromuscular organization and reveal that coordination between sensory and contractile systems in N. vectensis is …

Gun Violence Prevention In The Christian Church: A Deductive Content Analysis Of Faith-Based Firearm Education Resources, Sarah Winn

Graduate Theses and Dissertations

This thesis analyzed whether Christian faith-based curricula and toolkits regarding firearm safety and violence prevention align with established best practices in public health, social work, and education research. Previous research has explored the prevalence and impact of gun violence, best practices regarding gun violence and safety interventions, the effectiveness of churches as public health initiative partners, and the relationship between Christianity and views on firearms. In this study, qualitative deductive content analysis indicated that faith-based gun violence prevention toolkits are strongly oriented toward advocacy, community mobilization, and public health framing. At the same time, gaps remain in their integration of …

Creatures Of Habit? Impact Of Chick Feeder Tray Location On Feed Consumption At 0-7 Days Of Age, Emily Kate Green

Graduate Theses and Dissertations

Early chick nutrition is critical for establishing target growth performance characteristics in broiler chickens. This study evaluated factors within commercial broiler houses that potentially influence feed consumption during the first 7 days. Three trials were conducted in a 12.2 x 122 m commercial broiler house to assess the effects of house sectioning, spatial barriers, and feedline location. In Trial 1, a one-way experimental design was used in which the house was divided into four equal sections using migration fencing every 30.5 m. House sections served as a fixed factor. Feed was provided using chick trays placed beneath the feed line …

Caregiver Self-Reflection: Does It Matter? A Study Of The Relationship Between Caregiver Self-Reflection And Participation In Home Programs In Outpatient Pediatric Physical Therapy Settings, Andrea Myane

Graduate Theses and Dissertations

Despite evidence that caregiver coaching can improve patient outcomes and caregiver participation, little information exists on the effects of its individual components. Additionally, previous research has highlighted the importance of home programs in improving pediatric physical therapy outcomes. The aim of this study is to explore the role that caregiver self-reflection, a component of caregiver coaching, plays in facilitating outcomes in the pediatric physical therapy outpatient setting. Caregivers will be asked to complete a series of self-reflection questions based on Gibbs’ Reflective Cycle, while also recording the number of minutes they spend facilitating a prescribed home program. All study participants …

Conservation Agriculture Effects On Greenhouse Gas Emissions From Cotton, Soybean, And Corn In Southeast Arkansas, Jonathan Benjamin Brye

Graduate Theses and Dissertations

The slow degradation of natural resources has resulted in the focus of agriculture shifting towards conservation practices, such as reduced/no-tillage, cover crops (CC), and organic amendments (i.e., biochar), that conserve and improve natural resource quality. The objectives of this thesis were to i) quantify the impacts of biochar rate (i.e., 0, 2000, and 4000 kg ha-1) and ii) the combination of CC and tillage practice on carbon dioxide (CO¬2), methane (CH4), and nitrous oxide (N2O) fluxes, growing-season-long emissions, global warming potential (GWP), soil properties, and plant response from a minimally tilled cotton (Gossypium hirsutum)-corn (Zea mays) rotation and a soybean …

Searching Harder For The Darter: Evaluating Endangered Yellowcheek Darter (Nothonotus Moorei) Status And Drought Responses, Kearstin Findley

Graduate Theses and Dissertations

Freshwater ecosystems are one of the most imperiled habitats across the world with biodiversity declining rapidly. There are many stressors contributing to freshwater species declines including habitat loss and degradation, land-use changes, anthropogenic water demands, changes in precipitation and weather extremes, and pollution. Current environmental projections predict that these stressors will continue to impact freshwater ecosystems to greater extents moving forward. Stream drying from drought conditions increasing in intensity and frequency is especially concerning in northern Arkansas where seasonal drought is typical in the summer and fall months. With increased drying conditions, the elimination of shallow-water riffle habitats in streams …

Automated Circuit Design Algorithm And Implementation For Asynchronous Reset (Ares) Physically Unclonable Functions, Andrew Michael Felder

Graduate Theses and Dissertations

Technology is increasingly interwoven in all aspects of society as it solves new problems, creates new possibilities, and enables new conveniences. With its unceasing evolution, environments handling protected information such as military, finance, and medicine demand ever-evolving threat mitigation. Combating attackers’ abilities to spoof devices, uncover cryptographic keys, and bypass security features is a never-ending task which drives technological advancement to improve existing capabilities and develop entirely new methodologies. In this dissertation work, the hybrid Asynchronous RESet Physically Unclonable Function (ARES PUF) is evaluated at the circuit to determine its merits as a PUF. As part of this evaluation, results …

Deciphering Chemomechanical Couplings In Proteins Using Molecular Dynamics And Enhanced Sampling Techniques, Matthew Brownd

Graduate Theses and Dissertations

Proteins function through a complex interplay between chemical interactions and mechanical motions across multiple spatial and temporal scales. Understanding how ligand binding, conformational dynamics, and structural flexibility collectively regulate protein function remains one of the central challenges in molecular biophysics. This dissertation presents a computational investigation into chemomechanical coupling in three distinct classes of biomolecular systems using all-atom molecular dynamics (MD) simulations and enhanced sampling methods, with particular emphasis on free-energy calculations and long-timescale conformational analysis. The first part of this work focuses on ligand binding in hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which regulate rhythmic electrical activity in the heart …

Characterization Of Snrk1 Signaling In Rice Growth, Reproduction, And Stress Responses Using Phenotypic And Omics Analysis, Maria Clara Faria-Bates

Graduate Theses and Dissertations

SnRK1 is an evolutionarily conserved protein kinase belonging to the SNF1/AMPK family that is composed of a catalytic subunit (α) and two regulatory subunits (β and βγ), and it has a major role in adjusting growth in response to the energy status. SnRK1 phosphorylates and alters the activities of enzymes involved in metabolism and regulates gene expression by altering the activity of chromatin-remodeling enzymes or transcription factors. Rice contains three functional paralogs of SnRK1α: OsSnRK1αA (Os05g0530500), OsSnRK1αB (Os8g0484600), and OsSnRK1αC (Os03g0289100). Although SnRK1 is known to play a central role in plant growth and stress responses, the functional specialization of …

Navigating Mixedness As A Multiracial Teacher, Casey S. Kraichoke

Graduate Theses and Dissertations

This qualitative hermeneutic phenomenological study utilized Critical Multiracial Theory (MultiCrit) to construct a counternarrative that amplifies the voices of Multiracial teachers, focusing on the development of personal and teacher identity. Specifically, the study critically examines the trajectory of Multiethnic-Racial (ME-R) identity transformation following experiences teaching English as a Foreign Language (EFL) in participants’ heritage Asian countries. Data were collected through one-time semi-structured virtual interviews with fifteen purposefully selected Asian-White Multiracial EFL teachers who had completed a one-year Fulbright English Teaching Assistantship (ETA) within the past decade. Each participant also provided a written artifact to support the analysis. Participants reflected on …

Developmental Mathematics As A Potential Barrier To Degree Completion In Community Colleges, Cynthia Bernice Fletcher

Graduate Theses and Dissertations

Developmental mathematics is often seen as a barrier to student progression in community colleges, especially for students pursuing Associate of Science degrees requiring math coursework. This quantitative, ex post facto, non-experimental study examined how developmental mathematics factors predicted Associate of Science degree completion at a two-year community college in the West South-Central United States. Specifically, it assessed how academic performance in developmental mathematics, placement method, math pathway, and number of developmental math courses related to Associate of Science degree completion, as well as differences across student subgroups. Archival institutional data were used for first-time-in-college students across four cohorts: 2018, 2019, …

An Efficient Implementation Of Gibbs-Duhem Integration For Solid-Liquid Phase Coexistence In Binary Lennard-Jones System With Relevance To Aav Capsid Separation, Muhi Muntaka

Graduate Theses and Dissertations

Gene therapy using adeno-associated viruses (AAVs) holds promise for treating genetic diseases, but high manufacturing costs limit its widespread use. A major challenge is separating functional (full) AAV capsids from non-functional (empty) ones, which are produced in similar amounts and have nearly identical structures. Conventional purification methods, such as density gradient ultracentrifugation and chromatography, face drawbacks like low yield, poor scalability, and high cost, often requiring multi-step processes. Recently, selective crystallization has emerged as a promising alternative, offering improved efficiency, scalability, and product quality. However, optimizing this method requires a deep understanding of thermodynamic phase behavior in multicomponent systems. In …