Digital Commons Network^™

Mechanisms Underlying Diversity In Bacterial Biofilms, Jacob David Holt

Dartmouth College Ph.D Dissertations

Bacteria commonly exist in natural environments as communities of multiple strains and species attached to surfaces and encased in an extracellular matrix of DNA, protein, and polysaccharides–termed biofilms. These biofilms contribute to diverse functions, from ocean carbon cycling via chitin degradation to pathogenic processes such as wound or implant colonization. The molecular mechanisms governing biofilm formation and composition have been well characterized in model pathogenic organisms, yet the extent to which aspects of biofilm formation contribute to the assembly and maintenance of diverse communities remains less well understood. Using microfluidics, confocal microscopy, and model biofilm-forming bacteria, this thesis details how …

Stridevision: Automated Detection Of Running Form Deviations From 2d Pose Estimation And Machine Learning, Paulina Eguibar Ortega

Honors Theses

Running gait analysis plays a critical role in injury prevention and performance optimization, however, existing approaches often rely on specialized laboratory equipment or wearable sensors with limited interpretability. Recent advances in computer vision, particularly 2D human pose estimation, enable markerless motion analysis from standard video. However, progress remains constrained by the lack of publicly available datasets designed for running form analysis.

In this work, we introduce a preliminary dataset and benchmark for stride-level running gait analysis. The dataset consists of 73 treadmill running videos from 15 participants with varying experience levels, annotated with over 4,600 stride-level labels across multiple biomechanical …

Windows Of Developmental Susceptibility To Tetrahydrocannabivarin (Thcv) Reveal Stage-Dependent Morphological And Biological Effects, Mirielle Clayton

Honors Theses

Tetrahydrocannabivarin (THCV), a minor cannabinoid found in Cannabis sativa, is gaining attention for its potential therapeutic applications, yet its effects on early development remain poorly understood. This study investigated how the timing of THCV exposure influences morphological, behavioral, and bioaccumulation outcomes in zebrafish (Danio rerio) embryos. Embryos were exposed to 2 μM THCV during discrete developmental windows (6–30, 30–54, 54–78, 78–102) and continuous 6–102 hours post-fertilization (hpf), designed to model early stages of human gestation. Behavioral analysis using a larval photomotor response assay revealed significant reductions in locomotor activity during dark phases, with the strongest hypoactivity observed …

Expanding Expanded Access: A Case Of Onc201 And The Ethical Limitations Of Compassionate Use, Ava C. Hutchings

Honors Theses

This study examines the ethical and structural limitations of the Expanded Access program in the United States, with particular focus on its application to terminal pediatric illness. Although the program is intended to provide patients with life-threatening conditions access to investigational therapies outside of clinical trials, its effectiveness in serving critically ill children remains uncertain. This program is analyzed through careful consideration of regulatory review and ethical theory. Key policies governing Expanded Access are examined alongside scholarly discussions of pediatric autonomy and parental authority.

A case study of ONC201, a now-approved investigational drug, is used to illustrate how Expanded Access …

Evolution Of Nematode Prey Chemotaxis Behavior Across Different Environments, Hailey R. Smith

Honors Theses

Climatic conditions in the near future are projected to be warmer, with more frequent and intense weather events. This will substantially alter the environments in which organisms must integrate specific information to find essential resources. Many organisms rely upon chemical signals to orient towards attractive resources through a mechanism called chemotaxis. How different environmental conditions, like temperature, impact this process will be important to understand as environments change. I conducted a laboratory evolution experiment with the model nematode Caenorhabditis elegans to see how success in chemotaxis assays changes over time when selected under different temperature regimes (normal laboratory temperature, increased …

Values-Based Clinical Ethics: A New Model Of Health And Ethics Consultation For Patients Suffering In Extremis, Adam Trainer

Electronic Theses and Dissertations

This dissertation argues that contemporary clinical ethics is inadequately equipped to support patients suffering in extremis because it relies on an insufficiently discriminating account of health and a constrained model of moral reasoning. Drawing on Bernard Lonergan, it reconceives health as an emergent, multi-layered, heuristic notion shaped by biological, psychological, social, and existential goods. On that basis, it critiques principlism’s global applicability thesis and the undergirding claim that its principles form part of the common morality, arguing that this justificatory structure is philosophically insufficient and practically limited for clinical ethics in cases marked by profound suffering, moral plurality, and normative …

Hydrogeochemistry Of Natural Acid Rock Drainage In The Tobacco Root Mountains Of Southwest Montana And Implications For Restoration, Josie Grigsby

Honors Theses

This study investigates the hydrogeochemistry of the East Fork of the South Boulder River (EFSBR) in the northern Tobacco Root Mountains, Madison County, Montana. The upper 5 km of the main stem of the EFSBR is acidic (pH < 5.5), and dissolved concentrations of several trace metals including Al, Cd, Cu, and Zn exceed chronic aquatic life standards by 2-3 orders of magnitude. Unsurprisingly, the EFSBR appears to be devoid of trout. Since no mining has taken place at the source areas of the acidic headwater streams, this phenomenon is referred to as Natural Acid Rock Drainage (NARD). High mountains (up to 10,500’) bounding the EFSBR valley harbor a number of potential small rock glaciers and other periglacial features that appear to influence the hydrogeology and chemistry of the watershed. The steady flow of cold water in the hot summer months, during the same time period that neighboring drainages are drying up, is hypothesized to be sourced from melting ground ice high in the headwater catchment. Additionally, rock glaciers can act as efficient chemical reactors where they exist in conjunction with acid-generating mineralized bedrock, affecting the severity of acidic drainage and associated metal concentrations. Neutralization of the acidic water by alkaline tributaries results in the precipitation of aluminum hydroxysulfate (basaluminite), which covers the streambed with a vivid white mineral coating and can act as a natural sink through adsorption of other toxic metals to its surface. Pristine catchments exhibiting NARD can provide water quality data and serve as reference sites for restoration projects at sites experiencing Acid Mine Drainage where historical data does not exist. As climate change continues to alter the natural environment, especially in polar and high elevation regions, severity and instances of NARD are predicted to continue to increase on a global scale. A deeper understanding of the processes driving NARD from studies focused on areas such as the EFSBR will provide guidance for protecting vulnerable aquatic ecosystems and managing water resources in mountain communities into the future, as well as insights into necessary changes to established remediation methods in watersheds experiencing acid mine drainage.

Identifying Novel Gene Dysregulation Associated With Opioid Overdose Death: A Meta-Analysis Of Differential Gene Expression In Human Prefrontal Cortex, Javan K Carter, Bryan C Quach, Caryn Willis, Melyssa S Minto, Dana B Hancock, Janitza Montalvo-Ortiz, Olivia Corradin, Ryan W Logan, Consuelo Walss-Bass, Brion S Maher, Eric Otto Johnson

Faculty, Staff and Student Publications

Only recently have human postmortem brain studies of differential gene expression (DGE) associated with opioid overdose death (OOD) been published; sample sizes from these studies have been modest (N = 40-153). To increase statistical power to identify OOD-associated genes, we leveraged human prefrontal cortex RNA-seq data from four independent OOD studies and conducted a transcriptome-wide DGE meta-analysis (N = 272). Using a unified gene expression data processing and analysis framework across studies, we meta-analyzed 20, 098 genes and found 335 significant differentially expressed genes (DEGs) by OOD status (false discovery rate <  0.05). Of these, 66 DEGs were among the list of 303 genes reported as OOD-associated in prior prefrontal cortex molecular studies (e.g., genes/gene families OPRK1, NPAS4, DUSP, EGR). The remaining 269 DEGs were not previously reported (e.g., NR4A2, SYT1, HCRTR2, BDNF). There was little evidence of genetic drivers for the observed differences in gene expression between opioid addiction cases and controls. Enrichment analyses for the DEGs across molecular pathway and biological process databases highlight an interconnected set of genes and pathways linked to orexin and tyrosine kinase receptors through MEK/ERK/MAPK signaling to affect neuronal plasticity.

Progressive Cardiac Phenotypes And Reduced Reversibility From Long-Term Cugexp Rna Expression In A Dm1 Mouse Model, Rong-Chi Hu, Mohammadreza Tabary, Xander Ht Wehrens, Thomas A Cooper

Faculty, Staff and Students Publications

Myotonic dystrophy type 1 (DM1) is caused by an expanded CTG repeat in the DMPK gene, resulting in mutant transcripts that form expanded CUG (CUGexp) RNA foci and sequester muscleblind-like (MBNL) RNA-binding proteins. DM1 is multisystemic, with progressive worsening of disease manifestations in affected tissues. Disease progression is attributed to somatic expansion of the CTG repeats with age, resulting in production of CUGexp RNA with enhanced intrinsic toxicity due to increased MBNL sequestration. To determine the degree to which cardiac disease progression can occur independently of repeat expansion, we used a transgenic DM1 mouse model with inducible heart-specific expression of …

Conscientiousness And Collegiate Athletes: An Item Response Theory Analysis, Tyler Hall

College Honors Program

The purpose of this study is to examine how the facets (Kitchenham, L. et al., 2019) and aspects (Roberts et al., 2005) of conscientiousness influence collegiate athletes and how this understanding can be applied to the development of athletes and coaches. Like everyone else, student-athletes face daily stressors that can hinder their performance both in the classroom and on the field. Personality traits play a significant role in an individual's ability to manage these stressors (Lopes Dos Santos, M., 2020). This study examined conscientiousness, one of the Big Five Personality Traits defined by McCrae and Costa (1986), which is characterized …

Oxytocin And Vasopressin Receptors Mediate Dopamine Release In The Nucleus Accumbens, Rebecca Chari Crenshaw

Electronic Theses and Dissertations Archive

Oxytocin (OXT) is a potential therapeutic target for substance use disorders due to its modulatory effects on mesolimbic dopamine function. While OXT activity in the nucleus accumbens (NAc) influences dopamine release, the receptor mechanisms underlying these effects remain unclear. Oxytocin receptors (OXTRs) and vasopressin 1A receptors (V1aRs) are both expressed in the NAc and may differentially regulate dopamine function. This study examined how selective activation and blockade of OXTRs and V1aRs within the NAc modulate phasic and tonic dopamine release using in vivo amperometry in male and female mice. Intra-NAc OXT decreased phasic, but not tonic, dopamine release. Similarly, activation …

Inflammatory Markers In The Emergency Department And Ptsd Symptoms In The Aurora Study: A Longitudinal Cohort Study, Kristen Nishimi, Stacey L House, Et Al.

2020-Current year OA Pubs

BACKGROUND: Systemic inflammation is hypothesized to contribute to post-traumatic stress disorder (PTSD) vulnerability. Few studies have examined inflammation shortly after trauma as a predictor of later PTSD symptoms. We examined whether inflammation from the emergency department (ED) post-trauma is associated with PTSD symptom severity over the following 6 months.

METHODS: Our sample included 742 AURORA participants, a longitudinal cohort of patients in 29 EDs across the United States after a traumatic stressor, followed up to 6 months. Plasma cytokines were assessed from a study blood draw in the ED: an inflammatory index (standardized sum of generally pro-inflammatory markers interleukin [IL]-6, …

Development Of Novel Plasma Proteomic Biomarkers For Cancer-Associated Thrombosis In An Advanced Cancer Cohort, Preeti, Mrinal Ranjan, Dang Pham, Juan L Bueno, Micheline M Resende, Michael E Scheurer, Christopher I Amos, Chao Cheng, Ang Li

Faculty, Staff and Students Publications

Background: Existing risk models for cancer-associated thrombosis (CAT) show suboptimal performance in selective high-risk populations with cancer. Affinity-based plasma proteomics offers a novel approach for detecting CAT risk.

Objectives: To identify plasma biomarkers for CAT using proximity extension assays in an advanced cancer cohort.

Methods: We performed a nested case-control study using the Olink Explore HT panel. The final cohort included 57 patients with CAT and 113 matched control patients from 5 selected cancer types who had samples collected between cancer diagnosis and chemotherapy initiation. Random survival forest model was used to assess nonlinear associations with CAT in 5416 normalized …

Nivolumab Plus Ipilimumab For Chemotherapy-Refractory Metastatic Castration-Resistant Prostate Cancer: Results From The Randomized Portion Of The Phase 2 Checkmate 650 Trial, Padmanee Sharma, Russell K Pachynski, Et Al.

2020-Current year OA Pubs

We report on the randomized portion of the phase 2, open-label CheckMate 650 trial (NCT02985957), in which docetaxel-experienced, biologically male patients with chemotherapy-refractory metastatic castration-resistant prostate cancer were randomized 2:2:1:2 to nivolumab 3 mg /kg plus ipilimumab 1 mg /kg (n = 73), nivolumab 1 mg /kg plus ipilimumab 3 mg /kg (n = 74), ipilimumab 3 mg /kg (n = 38), or cabazitaxel (n = 74). Primary endpoints were objective response rate and radiographic progression-free survival; key secondary endpoints included overall survival, prostate-specific antigen response rate, and safety. This portion of CheckMate 650 was not designed to statistically compare …

Dna Methylation Signatures Associated With Bipolar Disorder In Peripheral Blood Improve Prediction Models, Markos Tesfaye, Anne-Kristin Stavrum, Kira D Höffler, Kevin S O'Connell, Friederike S David, Melanie E Garrett, Sonia Hesam-Shariati, Bronwyn J Overs, Claudia Pisanu, Luana Spano, Oliver J Watkeys, Antoine Weihs, Rafaella Ardau, Allison E Ashley-Koch, Lavinia Athanasiu, Jean C Beckham, Kyle J Bourassa, Caterina Chillotti, Srdjan Djurovic, Ole K Drange, Josef Frank, Anouar Khayachi, Nathan A Kimbrel, Lourdes Martorell, Susanne Meinert, Ingrid Melle, Gunnar Morken, Pasquale Paribello, Marco Pinna, Gloria Roberts, Guy Rouleau, Peter R Schofield, Esteban Sepúlveda, Giovanni Severino, Vidar M Steen, Frederike Stein, Fabian Streit, Va Mid-Atlantic Mirecc Workgroup, Trine Vik Lagerberg, Martin Alda, Udo Dannlowski, Andreas J Forstner, Janice M Fullerton, Hans J Grabe, Melissa J Green, Tilo Kircher, Javier Labad, Mirko Manchia, Philip B Mitchell, Jair C Soares, Alessio Squassina, Alexander Teumer, Leonardo Tondo, Elisabet Vilella, Ole A Andreassen, Boris Chaumette, Gabriel R Fries, Stephanie Le Hellard

Faculty, Staff and Student Publications

Background: Bipolar disorder (BD) is a major mood disorder influenced by both genetic and environmental factors. While DNA methylation from peripheral tissues can reflect both genetic and environmental influences and reveal insights into disease biology, it remains understudied in BD. DNA methylation signatures may complement polygenic scores (PGS) and hold potential as biomarkers. Here, we conducted the largest epigenome-wide association study (EWAS) of BD to date and evaluated the predictive value of polymethylation scores (PMS) in classifying case-control status.

Methods: DNA methylation from peripheral blood of 1729 cases and 1747 controls, comprising twelve cohorts, was obtained. We performed meta-analyses for …

Tunable Tripcides Suppress Virulence Factor Secretion During Staphylococcus Aureus Infection And Kill Dormant Cells, Hasan Tükenmez, Taylor M Nye, Suzanne Hickerson, Chloe L P Obernuefemann, Jerome S Pinkner, Karen W Dodson, Michael G Caparon, Scott J Hultgren, Et Al.

2020-Current year OA Pubs

Antimicrobial resistance (AMR) in common bacterial pathogens, including methicillin-resistant

Legalitas Perkawinan Antar Pemeluk Beda Agama Di Indonesia Dikaitkan Dengan Undang-Undang No.1 Tahun 1974 Tentang Perkawinan, Hutari H.W.P.

Jurnal Hukum & Pembangunan

Abstract

Rancangan Undang-Undang Antipornografi Dan Pornoaksi Sebagai Integrasi Kebudayaan Sosial Yang Dipaksakan, Ridha Sjartina

Jurnal Hukum & Pembangunan

Abstract

The Genus Nebo Simon, 1878 (Scorpiones: Diplocentridae) In Oman, Graeme Lowe

Euscorpius

No abstract provided.

In The Garden's Grasp, Lacey Anderson

The Cicadian

This piece pairs a close-up photograph with reflective creative writing to explore a fleeting moment in a backyard garden ecosystem. Among vivid dahlias and winding vines, a grasshopper emerges, captured both in image and in story. Together, the photograph and prose invite readers to witness how tending, noticing, and lingering can transform an ordinary patio into a world alive with quiet, electric life.

Nature Writing As A Bridge To Student Scientific Interest, Christine Mcgrail, John Kudukey, Colette Smith, Fnu Dharma

The Cicadian

Nature writing is an important pathway for preservice teachers’ engagement with science that can help more students recognize themselves as knowers and doers of science and better connect them and their students to the world around them. Nature writing allows all students, including those from non-dominant cultural backgrounds, to include their experiences and backgrounds in their science education. Nature writing also affords all students an opportunity to better understand the natural world they are a part of. Nature writing is typically written in first person and characterized as scientific observation of the natural world through a personal reflective lens. In …

Hormonal Seed Primimg As A Sustainable Alternative To Pesticides, Oyekemi Oyetoro, Swayamjit Ray

Research and Creative Works Expo

This study investigates the use of hormonal seed priming with Methyl Jasmonate (MeJA) and Methyl Salicylic Acid (MeSA) as a sustainable alternative to synthetic pesticides. By inducing Systemic Acquired Resistance (SAR) and Induced Systemic Resistance (ISR), these treatments aim to prepare plant defenses for rapid response to future herbivory with reduced reliance on chemical applications. The research specifically explores the growth-defense trade off, evaluating how various hormonal concentrations impact both pest resistance and seedling vigor. The findings identify optimal priming protocols that balance robust protection against herbivores with the maintenance of primary vegetative growth

Are There Gender Disparities In Chinese Legal Education? – An Empirical Enquiry, Peter C.H. Chan, Haojin Duan

Penn State Journal of Law & International Affairs (2012 - Present)

No abstract provided.

Attention Please, This Island Earth, Diane Ackerman

Creative Nonfiction Magazine

No abstract provided.

Notes On My Dying, Ruthann Robson

Creative Nonfiction Magazine

No abstract provided.

Brine Solidification And Metals Sequestration Using Lignocellulosics, Frank Kvietok

World of Coal Ash Proceedings

Coal ash has historically been used as a convenient, on-site solidification material for brines generated from flue gas desulfurization at power plants. As alternate utilization scenarios are developed for coal ash, lignocellulosic (wood-based) sorbents can serve as a highly efficient substitute for the solidification process. Results will be shared for a variety of different brine types: thermally reduced, thermally reduced and neutralized, and reverse osmosis. In addition to solidification performance, which demonstrated a 3-4x mass of liquid vs mass of sorbent factor, studies were conducted to quantify the strength of binding to the wood-based sorbent for a broad range of …

Cyberattack-Resilient Artificial Intelligence In Navies—Solving The Skill-Needs Gap, Chris C. Demchak

Naval War College Review

With artificial intelligence—cyber’s offspring—come several risks and vulnerabilities that must be defended against. The key to defense, as always, is having the right personnel in place to build resilience into the mission. To distribute critical manning assets correctly, skill-needs rubrics are well suited to ensure that the proper personnel are on hand to defend systems and execute critical missions.

Newsletter

Lloyd Clark Papers

Page from The Alumni Update, the UTA Alumni Association Newsletter, from Summer 1999.

Lessons Learned: Commissioning An Fgd Wastewater Treatment System, Bella Triolet, Mckale Grant, Louis Manigault

World of Coal Ash Proceedings

Stantec recently supported commissioning a flue gas desulfurization (FGD) Wastewater Treatment System in accordance with the 2020 Environmental Protection Agency (EPA) Effluent Limitations Guidelines (ELG) Rule, which requires that coal fired power plants meet their more stringent requirements by December 31, 2025. With the addition of the 2024 EPA ELG Rule, coal fired power plants will now be expected to move to zero liquid discharge (ZLD) systems or permanently cease coal combustion by 2034. Due to this change, it is likely that coal fired power plants will be looking to design and install ZLD systems within the next decade. While …

Jacksonian Democracy: Triumph Or Tragedy?, Gabe Blumer

Undergraduate Theses and Senior Projects

This capstone paper analyzes Andrew Jackson's presidential impact on democracy and discusses its strengths and weaknesses as a whole. It goes over the impact on white men, black Americans, and Native Americans in particular.